AlfalfaGEDB | MsG0180005526.01

M. sativa cultivar ZhongmuNo.1 / MsG0180005526.01

KEGG: -
GO: GO:0046983
PFAM: PF00010

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005526.01.T01	XP_003592388.2	96.667	60	2	0	1	60	77	136	5.60E-32	120

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005526.01.T01	Q9FLI1	73.333	60	16	0	1	60	4	63	3.03E-26	96.7

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005526.01.T01	G7ID49	96.667	60	2	0	1	60	77	136	2.67e-32	120

TFs/TRs:

Gene ID	Type	Classification
MsG0180005526.01.T01	TF	bHLH

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005526.01.T01	MTR_1g102370	96.667	60	2	0	1	60	77	136	6.78e-36	120
MsG0180005526.01.T01	MTR_0007s0380	75.000	60	15	0	1	60	73	132	2.51e-26	95.9
MsG0180005526.01.T01	MTR_0007s0330	57.627	59	25	0	2	60	9	67	5.49e-19	75.5
MsG0180005526.01.T01	MTR_0007s0330	57.627	59	25	0	2	60	21	79	1.33e-18	75.1
MsG0180005526.01.T01	MTR_1g102380	55.000	60	27	0	1	60	21	80	5.11e-17	70.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005526.01.T01	AT5G51780	73.333	60	16	0	1	60	4	63	3.09e-27	96.7
MsG0180005526.01.T01	AT5G51780	73.333	60	16	0	1	60	27	86	4.74e-27	97.1
MsG0180005526.01.T01	AT4G25400	65.000	60	21	0	1	60	27	86	2.11e-23	87.4
MsG0180005526.01.T01	AT4G25400	65.000	60	21	0	1	60	27	86	2.54e-23	87.0
MsG0180005526.01.T01	AT4G25410	58.621	58	24	0	3	60	47	104	3.07e-18	74.7
MsG0180005526.01.T01	AT5G51790	55.000	60	27	0	1	60	29	88	2.84e-17	71.6
MsG0180005526.01.T01	AT5G51790	55.000	60	27	0	1	60	29	88	3.15e-17	71.6
MsG0180005526.01.T01	AT5G51790	55.000	60	27	0	1	60	94	153	7.37e-17	71.6
MsG0180005526.01.T01	AT5G51790	55.000	60	27	0	1	60	94	153	7.79e-17	71.6
MsG0180005526.01.T01	AT1G62975	53.333	60	28	0	1	60	76	135	5.29e-16	69.3
MsG0180005526.01.T01	AT1G62975	53.333	60	28	0	1	60	76	135	5.81e-16	69.3
MsG0180005526.01.T01	AT1G12540	55.172	58	26	0	3	60	79	136	2.50e-14	64.7

Find 15 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGAGGGAGAAGGGATCTA+AGG	0.225427	1:-92884001	None:intergenic
ATAAGAAGAAAATGGTTCAT+AGG	0.350975	1:+92883931	None:intergenic
AAATCACTTGAAGAAGAATA+TGG	0.357120	1:+92884171	MsG0180005526.01.T01:CDS
AAGAGGGAGAAGGGATCTAA+GGG	0.467872	1:-92884000	None:intergenic
TAAGAAGAAAATGGTTCATA+GGG	0.475156	1:+92883932	None:intergenic
TGGAAGAACTAAGTTACAAA+GGG	0.507887	1:+92884191	MsG0180005526.01.T01:CDS
TTTATCTGATCAAATGAATG+AGG	0.525114	1:+92884135	MsG0180005526.01.T01:CDS
GAAGCAAAGGAGACAAGAAA+TGG	0.533586	1:+92883962	MsG0180005526.01.T01:CDS
TCTCCCTCTTCACTTCATCA+AGG	0.533778	1:+92884013	MsG0180005526.01.T01:CDS
TCACCTTGATGAAGTGAAGA+GGG	0.538188	1:-92884016	None:intergenic
GATGAAGTGAAGAGGGAGAA+GGG	0.546212	1:-92884009	None:intergenic
ATGGAAGAACTAAGTTACAA+AGG	0.563462	1:+92884190	MsG0180005526.01.T01:CDS
ATAGGGAAATTGAGAAGCAA+AGG	0.575199	1:+92883949	MsG0180005526.01.T01:CDS
TGATGAAGTGAAGAGGGAGA+AGG	0.577558	1:-92884010	None:intergenic
TTCACCTTGATGAAGTGAAG+AGG	0.603402	1:-92884017	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTAATCATTAAAGTTTTCT+AGG	+	Chr1:92884055-92884074	MsG0180005526.01.T01:intron	15.0%
!!	TATCATCTGTTATAACTTTA+GGG	+	Chr1:92884079-92884098	MsG0180005526.01.T01:intron	20.0%
!!	TTATCATCTGTTATAACTTT+AGG	+	Chr1:92884078-92884097	MsG0180005526.01.T01:intron	20.0%
!!!	TATTTATTGTTTCTCTAACA+GGG	+	Chr1:92884103-92884122	MsG0180005526.01.T01:intron	20.0%
!!!	TTATTTATTGTTTCTCTAAC+AGG	+	Chr1:92884102-92884121	MsG0180005526.01.T01:intron	20.0%
!	AAATCACTTGAAGAAGAATA+TGG	+	Chr1:92884171-92884190	MsG0180005526.01.T01:CDS	25.0%
!	TTTATCTGATCAAATGAATG+AGG	+	Chr1:92884135-92884154	MsG0180005526.01.T01:CDS	25.0%
	ATGGAAGAACTAAGTTACAA+AGG	+	Chr1:92884190-92884209	MsG0180005526.01.T01:CDS	30.0%
	TGGAAGAACTAAGTTACAAA+GGG	+	Chr1:92884191-92884210	MsG0180005526.01.T01:CDS	30.0%
	ATAGGGAAATTGAGAAGCAA+AGG	+	Chr1:92883949-92883968	MsG0180005526.01.T01:CDS	35.0%
	GAAGCAAAGGAGACAAGAAA+TGG	+	Chr1:92883962-92883981	MsG0180005526.01.T01:CDS	40.0%
	TCACCTTGATGAAGTGAAGA+GGG	-	Chr1:92884019-92884038	None:intergenic	40.0%
	TTCACCTTGATGAAGTGAAG+AGG	-	Chr1:92884020-92884039	None:intergenic	40.0%
	AAGAGGGAGAAGGGATCTAA+GGG	-	Chr1:92884003-92884022	None:intergenic	45.0%
	GATGAAGTGAAGAGGGAGAA+GGG	-	Chr1:92884012-92884031	None:intergenic	45.0%
	TCTCCCTCTTCACTTCATCA+AGG	+	Chr1:92884013-92884032	MsG0180005526.01.T01:CDS	45.0%
	TGATGAAGTGAAGAGGGAGA+AGG	-	Chr1:92884013-92884032	None:intergenic	45.0%
	GAAGAGGGAGAAGGGATCTA+AGG	-	Chr1:92884004-92884023	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	92883942	92884222	92883942	ID=MsG0180005526.01;Name=MsG0180005526.01
Chr1	mRNA	92883942	92884222	92883942	ID=MsG0180005526.01.T01;Parent=MsG0180005526.01;Name=MsG0180005526.01.T01;_AED=0.46;_eAED=0.46;_QI=0\|0\|0\|1\|0\|0\|2\|0\|63
Chr1	exon	92883942	92884034	92883942	ID=MsG0180005526.01.T01:exon:49857;Parent=MsG0180005526.01.T01
Chr1	exon	92884124	92884222	92884124	ID=MsG0180005526.01.T01:exon:49858;Parent=MsG0180005526.01.T01
Chr1	CDS	92883942	92884034	92883942	ID=MsG0180005526.01.T01:cds;Parent=MsG0180005526.01.T01
Chr1	CDS	92884124	92884222	92884124	ID=MsG0180005526.01.T01:cds;Parent=MsG0180005526.01.T01

Gene Sequence	>MsG0180005526.01.T01 ATGGTTCATAGGGAAATTGAGAAGCAAAGGAGACAAGAAATGGCTACACTTCATGCTTCCCTTAGATCCCTTCTCCCTCTTCACTTCATCAAGGGAAAACGTTCTTTATCTGATCAAATGAATGAGGCAGTGAATTACATAAATCACTTGAAGAAGAATATGGAAGAACTAAGTTACAAAGGGATGAACTGA
Protein sequence	>MsG0180005526.01.T01 MVHREIEKQRRQEMATLHASLRSLLPLHFIKGKRSLSDQMNEAVNYINHLKKNMEELSYKGMN*

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