AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005770.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005770.01.T01 MTR_1g106420 82.734 139 8 2 34 156 56 194 9.31e-71 211
MsG0180005770.01.T01 MTR_5g015880 54.667 75 31 2 29 103 52 123 2.35e-20 84.0
MsG0180005770.01.T01 MTR_4g095600 63.333 60 20 1 35 94 58 115 5.01e-19 80.5
MsG0180005770.01.T01 MTR_7g007010 61.905 42 16 0 40 81 65 106 2.90e-14 68.9
MsG0180005770.01.T01 MTR_6g018270 59.524 42 17 0 40 81 65 106 1.25e-13 66.6
MsG0180005770.01.T01 MTR_6g453130 56.522 46 20 0 36 81 63 108 6.22e-12 61.6
MsG0180005770.01.T01 MTR_1g110840 40.299 67 35 1 36 102 63 124 8.19e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005770.01.T01 AT3G49940 62.687 67 21 2 31 95 52 116 6.39e-21 85.9
MsG0180005770.01.T01 AT5G67420 58.462 65 23 2 31 93 52 114 4.81e-19 80.9
MsG0180005770.01.T01 AT5G67420 58.462 65 23 2 31 93 52 114 4.81e-19 80.9
MsG0180005770.01.T01 AT4G37540 47.115 104 39 5 40 137 63 156 1.27e-18 79.7
MsG0180005770.01.T01 AT1G67100 58.140 43 18 0 40 82 65 107 1.17e-13 66.2
MsG0180005770.01.T01 AT1G68510 36.735 98 58 2 40 137 65 158 1.64e-13 65.9
MsG0180005770.01.T01 AT3G02550 57.143 42 18 0 40 81 65 106 4.12e-13 65.1

Find 42 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGAAATTACAGTCAGAATT+TGG 0.204968 1:-95979710 None:intergenic
AGCCCAACTGCTCCATTTAC+TGG 0.215944 1:-95981172 None:intergenic
AGAACTATAAATCCAGTAAA+TGG 0.325401 1:+95981160 MsG0180005770.01.T01:CDS
ATGGAGCAGTTGGGCTGTTA+TGG 0.333353 1:+95981179 MsG0180005770.01.T01:CDS
AACTAGTGCCCCGGGGCAAC+TGG 0.403064 1:+95979646 MsG0180005770.01.T01:intron
AATGAGTCATTTAAGCATGT+TGG 0.441033 1:+95977566 MsG0180005770.01.T01:CDS
CACTTATGTATGAAGCTGTT+GGG 0.452221 1:+95981137 MsG0180005770.01.T01:CDS
GACACATGAGTCTGAGGAAT+CGG 0.458754 1:+95981357 MsG0180005770.01.T01:CDS
TCTTGTTAACCAGTTGCCCC+GGG 0.460661 1:-95979655 None:intergenic
TCCACACCTAACAACTGATC+AGG 0.464754 1:-95981277 None:intergenic
AATCCAGTAAATGGAGCAGT+TGG 0.467406 1:+95981169 MsG0180005770.01.T01:CDS
GAATCGGAGTCGTCCACGTT+GGG 0.467550 1:+95981373 MsG0180005770.01.T01:CDS
GTCTTGTTAACCAGTTGCCC+CGG 0.470082 1:-95979656 None:intergenic
CAATTGCGATATTTATCCAT+AGG 0.471432 1:-95979679 None:intergenic
TGTTTCCTTAACTAGTGCCC+CGG 0.472785 1:+95979637 MsG0180005770.01.T01:intron
GCACTCCCTGATCAGTTGTT+AGG 0.477941 1:+95981271 MsG0180005770.01.T01:CDS
GTTTCCTTAACTAGTGCCCC+GGG 0.494127 1:+95979638 MsG0180005770.01.T01:intron
CCCTGATCAGTTGTTAGGTG+TGG 0.497837 1:+95981276 MsG0180005770.01.T01:CDS
AATTGCGATATTTATCCATA+GGG 0.524561 1:-95979678 None:intergenic
ATCCAGTAAATGGAGCAGTT+GGG 0.536591 1:+95981170 MsG0180005770.01.T01:CDS
TCACTTATGTATGAAGCTGT+TGG 0.539852 1:+95981136 MsG0180005770.01.T01:CDS
GAACAAGTGCTGAGAGGTAA+CGG 0.548928 1:+95981235 MsG0180005770.01.T01:CDS
ATGCTAATTCTCAAACTCAA+AGG 0.552063 1:+95981431 MsG0180005770.01.T01:CDS
GAGATCATAAGAATAGTGTA+AGG 0.555999 1:-95981462 None:intergenic
ATAAGAATAGTGTAAGGAGT+TGG 0.568732 1:-95981456 None:intergenic
CAGCTTCATACATAAGTGAC+TGG 0.569517 1:-95981132 None:intergenic
AACTGGTTAACAAGACCCTA+TGG 0.580630 1:+95979663 MsG0180005770.01.T01:CDS
GGCTGTTATGGACTGGAAAG+TGG 0.603769 1:+95981191 MsG0180005770.01.T01:CDS
GCAGTTGGGCTGTTATGGAC+TGG 0.615948 1:+95981184 MsG0180005770.01.T01:CDS
CCACACCTAACAACTGATCA+GGG 0.634962 1:-95981276 None:intergenic
TGCGGAGACACATGAGTCTG+AGG 0.638167 1:+95981351 MsG0180005770.01.T01:CDS
GGAATCGGAGTCGTCCACGT+TGG 0.646426 1:+95981372 MsG0180005770.01.T01:CDS
AAGTGGCAATTCTGTCAACT+TGG 0.652925 1:+95981208 MsG0180005770.01.T01:CDS
TTCTGTTCTACTTCCCAACG+TGG 0.659625 1:-95981386 None:intergenic
ACAGTCAGAATTTGGATACG+TGG 0.663957 1:-95979702 None:intergenic
ACTGGCGTAAGGAGGATGCG+CGG 0.670752 1:+95977539 None:intergenic
CTTGTTAACCAGTTGCCCCG+GGG 0.676943 1:-95979654 None:intergenic
TAACAACTGATCAGGGAGTG+CGG 0.677605 1:-95981269 None:intergenic
CCAAGCAGAAAAGCAACGTG+CGG 0.683012 1:+95981333 MsG0180005770.01.T01:CDS
TTTCCTTAACTAGTGCCCCG+GGG 0.712632 1:+95979639 MsG0180005770.01.T01:intron
GGTGTTGAACAAGTGCTGAG+AGG 0.756300 1:+95981229 MsG0180005770.01.T01:CDS
CAAGTGCTGAGAGGTAACGG+CGG 0.757236 1:+95981238 MsG0180005770.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTAATTTTTAATAAAT+TGG + Chr1:95979886-95979905 MsG0180005770.01.T01:intron 0.0%
!! TAGTAATATTAATTAAATCA+AGG + Chr1:95978613-95978632 MsG0180005770.01.T01:intron 10.0%
!! AATAAAAATAAAGCTATAGA+TGG + Chr1:95977923-95977942 MsG0180005770.01.T01:intron 15.0%
!! AATATATATGGTTAATGATT+TGG + Chr1:95977986-95978005 MsG0180005770.01.T01:intron 15.0%
!! AATCATTAACCATATATATT+TGG - Chr1:95977986-95978005 None:intergenic 15.0%
!! ACTTAATTAAGCATAATATA+TGG - Chr1:95980622-95980641 None:intergenic 15.0%
!! ATTAACTTATATGTAGTTAA+AGG - Chr1:95979352-95979371 None:intergenic 15.0%
!! ATTATTAATACTTACTACTA+AGG - Chr1:95978700-95978719 None:intergenic 15.0%
!! GTAGTAAGTATTAATAATAA+TGG + Chr1:95978702-95978721 MsG0180005770.01.T01:intron 15.0%
!! TAAAATGATTTAGTATATCA+AGG + Chr1:95978127-95978146 MsG0180005770.01.T01:intron 15.0%
!! TATGGTATTAGTATATATAA+TGG - Chr1:95980604-95980623 None:intergenic 15.0%
!!! AAAATCTTGATTTTAGATTT+TGG - Chr1:95978839-95978858 None:intergenic 15.0%
!!! AACTTAATTAAAAGTATTAC+TGG + Chr1:95979251-95979270 MsG0180005770.01.T01:intron 15.0%
!!! ACTAATTTGTATGTATATAT+AGG + Chr1:95981029-95981048 MsG0180005770.01.T01:intron 15.0%
!!! ACTTAATTAAAAGTATTACT+GGG + Chr1:95979252-95979271 MsG0180005770.01.T01:intron 15.0%
!!! ACTTTTAATTAAGTTTGATT+AGG - Chr1:95979246-95979265 None:intergenic 15.0%
!! AAAATTAATACTATACCACT+TGG + Chr1:95979318-95979337 MsG0180005770.01.T01:intron 20.0%
!! AAACACAAATAAATAGTGTT+TGG - Chr1:95978179-95978198 None:intergenic 20.0%
!! ATGGTTAAGTTACAAATATT+GGG - Chr1:95980488-95980507 None:intergenic 20.0%
!! ATTCAATTACTCTATATTAC+AGG + Chr1:95981103-95981122 MsG0180005770.01.T01:intron 20.0%
!! GTATATTTAAATTGGAAATC+TGG - Chr1:95979290-95979309 None:intergenic 20.0%
!! TAAAAATGTAACACTGTATA+TGG - Chr1:95980706-95980725 None:intergenic 20.0%
!! TATAATAAAATGAGTTGCAA+CGG + Chr1:95980765-95980784 MsG0180005770.01.T01:intron 20.0%
!! TATATATAATGGTGAAAAGT+AGG - Chr1:95980593-95980612 None:intergenic 20.0%
!! TATATGGTCTTTATATCTTA+AGG - Chr1:95980690-95980709 None:intergenic 20.0%
!!! AACATTAATTTTGTTGGAAT+TGG + Chr1:95979438-95979457 MsG0180005770.01.T01:intron 20.0%
!!! AATTTTAAAGCATGTTTATG+TGG - Chr1:95978501-95978520 None:intergenic 20.0%
!!! ACAAATTAGTTTGATGTATT+TGG - Chr1:95981019-95981038 None:intergenic 20.0%
!!! CTGCATATACTTTAATTTAA+CGG + Chr1:95978202-95978221 MsG0180005770.01.T01:intron 20.0%
!!! CTTAATTAAAAGTATTACTG+GGG + Chr1:95979253-95979272 MsG0180005770.01.T01:intron 20.0%
!!! GTACATTATTTCTGATAAAA+TGG + Chr1:95979091-95979110 MsG0180005770.01.T01:intron 20.0%
!!! TTTTACTGTATTCACTAATT+TGG - Chr1:95980326-95980345 None:intergenic 20.0%
!!! TTTTTATGACAGAAAAGATT+AGG + Chr1:95980242-95980261 MsG0180005770.01.T01:intron 20.0%
! AAATTAATGTTTGTGGAGAA+TGG - Chr1:95979429-95979448 None:intergenic 25.0%
! AATACAGTAAAATTTGATGG+TGG + Chr1:95980334-95980353 MsG0180005770.01.T01:intron 25.0%
! AATTAATGTTTGTGGAGAAT+GGG - Chr1:95979428-95979447 None:intergenic 25.0%
! AATTGCGATATTTATCCATA+GGG - Chr1:95979681-95979700 None:intergenic 25.0%
! ACTGTTTCAAAAAGAAAATC+AGG - Chr1:95979073-95979092 None:intergenic 25.0%
! AGAACTATAAATCCAGTAAA+TGG + Chr1:95981160-95981179 MsG0180005770.01.T01:CDS 25.0%
! ATGCCATTCAAAATATAAAG+TGG + Chr1:95980167-95980186 MsG0180005770.01.T01:intron 25.0%
! CAATATTTGTAACTTAACCA+TGG + Chr1:95980487-95980506 MsG0180005770.01.T01:intron 25.0%
! CATGGTTAAGTTACAAATAT+TGG - Chr1:95980489-95980508 None:intergenic 25.0%
! GTGAATACAGTAAAATTTGA+TGG + Chr1:95980331-95980350 MsG0180005770.01.T01:intron 25.0%
! TAAGTTACAAATATTGGGTA+GGG - Chr1:95980483-95980502 None:intergenic 25.0%
! TAGAAATTACAGTCAGAATT+TGG - Chr1:95979713-95979732 None:intergenic 25.0%
! TAGTGTATATATTCAAACCA+TGG - Chr1:95980507-95980526 None:intergenic 25.0%
! TATATGACATACATGATCTT+TGG - Chr1:95980950-95980969 None:intergenic 25.0%
! TCCAACAAAATTAATGTTTG+TGG - Chr1:95979436-95979455 None:intergenic 25.0%
! TGCCATTCAAAATATAAAGT+GGG + Chr1:95980168-95980187 MsG0180005770.01.T01:intron 25.0%
! TTAAGTTACAAATATTGGGT+AGG - Chr1:95980484-95980503 None:intergenic 25.0%
! TTAGTTAATCATGTGAAGAA+AGG - Chr1:95980647-95980666 None:intergenic 25.0%
!! AACATTTTCCTTATCCATAA+TGG - Chr1:95979497-95979516 None:intergenic 25.0%
!! AGATTTTGCGTTTATTACAA+AGG + Chr1:95978852-95978871 MsG0180005770.01.T01:intron 25.0%
!! ATACAGTAAAATTTGATGGT+GGG + Chr1:95980335-95980354 MsG0180005770.01.T01:intron 25.0%
!! GATTTTGCGTTTATTACAAA+GGG + Chr1:95978853-95978872 MsG0180005770.01.T01:intron 25.0%
!! TCCACAAACATTAATTTTGT+TGG + Chr1:95979432-95979451 MsG0180005770.01.T01:intron 25.0%
!!! AAATTAGTTTGTGAAATGCA+AGG - Chr1:95979209-95979228 None:intergenic 25.0%
!!! TAGGAGTATTTTTTTTCTAG+AGG - Chr1:95980096-95980115 None:intergenic 25.0%
!!! TTTTTCTAGAGGAGATTTAT+AGG - Chr1:95980085-95980104 None:intergenic 25.0%
AAAGAGCAATAGTTATAGCA+TGG - Chr1:95977853-95977872 None:intergenic 30.0%
AAGTATTACTGGGGTTATAA+TGG + Chr1:95979262-95979281 MsG0180005770.01.T01:intron 30.0%
AATGAGTCATTTAAGCATGT+TGG + Chr1:95977566-95977585 MsG0180005770.01.T01:CDS 30.0%
AATTGACGAGAATAAGAGTA+AGG + Chr1:95979926-95979945 MsG0180005770.01.T01:intron 30.0%
AGTAAATAATCTCACTCTCA+CGG - Chr1:95978901-95978920 None:intergenic 30.0%
AGTATTACTGGGGTTATAAT+GGG + Chr1:95979263-95979282 MsG0180005770.01.T01:intron 30.0%
ATAAGAATAGTGTAAGGAGT+TGG - Chr1:95981459-95981478 None:intergenic 30.0%
ATAGCTAGCTAGTAATAGAT+AGG - Chr1:95980011-95980030 None:intergenic 30.0%
ATGCACAAGTTTATCCTAAA+TGG + Chr1:95979399-95979418 MsG0180005770.01.T01:intron 30.0%
ATGCTAATTCTCAAACTCAA+AGG + Chr1:95981431-95981450 MsG0180005770.01.T01:CDS 30.0%
CAAGTGTGTCCAAATATATA+TGG + Chr1:95977974-95977993 MsG0180005770.01.T01:intron 30.0%
CAATTGCGATATTTATCCAT+AGG - Chr1:95979682-95979701 None:intergenic 30.0%
GCCATTCAAAATATAAAGTG+GGG + Chr1:95980169-95980188 MsG0180005770.01.T01:intron 30.0%
GCTAGAAACAGTAAACAAAT+GGG - Chr1:95978443-95978462 None:intergenic 30.0%
TAGTTACAACTGAAGTTAAC+AGG - Chr1:95978770-95978789 None:intergenic 30.0%
TGAAATGCAAGGACAATTAT+TGG - Chr1:95979198-95979217 None:intergenic 30.0%
TGCTAGAAACAGTAAACAAA+TGG - Chr1:95978444-95978463 None:intergenic 30.0%
TGCTAGCTGCATATATTTAT+AGG - Chr1:95979738-95979757 None:intergenic 30.0%
TTTACAACTCATCTTGAAGT+AGG + Chr1:95979850-95979869 MsG0180005770.01.T01:intron 30.0%
! AATTTTTCCACACTTGACTT+GGG - Chr1:95980383-95980402 None:intergenic 30.0%
! ACTACTTTGTTTCTTTCACA+AGG + Chr1:95978786-95978805 MsG0180005770.01.T01:intron 30.0%
! ATTGATAATTACTGCCAATC+CGG + Chr1:95978533-95978552 MsG0180005770.01.T01:intron 30.0%
! CATGTTTATGTGGATTTTAG+TGG - Chr1:95978491-95978510 None:intergenic 30.0%
! TACTTTAATTTAACGGTCAC+TGG + Chr1:95978209-95978228 MsG0180005770.01.T01:intron 30.0%
! TAGAACTAGAAGTCTCATTT+TGG - Chr1:95978306-95978325 None:intergenic 30.0%
! TATTTTTCGCAATTGTAGAG+AGG + Chr1:95978262-95978281 MsG0180005770.01.T01:intron 30.0%
!! AAAAGTTGGTATGTGGTTAA+TGG - Chr1:95978562-95978581 None:intergenic 30.0%
!! AAAGTTGGTATGTGGTTAAT+GGG - Chr1:95978561-95978580 None:intergenic 30.0%
!! ACCCCACTTTATATTTTGAA+TGG - Chr1:95980173-95980192 None:intergenic 30.0%
!!! AGTTGTTTTGTTTTTCCCTT+TGG - Chr1:95978351-95978370 None:intergenic 30.0%
!!! ATGGCTTGTTTTTTAGCTTA+GGG - Chr1:95977727-95977746 None:intergenic 30.0%
!!! GCTTTGATGTGACAATTTTT+TGG - Chr1:95977767-95977786 None:intergenic 30.0%
!!! TGATGTGACAATTTTTTGGT+GGG - Chr1:95977763-95977782 None:intergenic 30.0%
!!! TTGATGTGACAATTTTTTGG+TGG - Chr1:95977764-95977783 None:intergenic 30.0%
AAACAAACAGCATGAACCAA+AGG + Chr1:95978332-95978351 MsG0180005770.01.T01:intron 35.0%
AAACACAATTGAACAGTGCT+TGG + Chr1:95978057-95978076 MsG0180005770.01.T01:intron 35.0%
AACAAACAGCATGAACCAAA+GGG + Chr1:95978333-95978352 MsG0180005770.01.T01:intron 35.0%
AGGTGAAAGAAAAGACATGA+GGG - Chr1:95980916-95980935 None:intergenic 35.0%
ATAATCCACCCTAGAAAAGT+TGG - Chr1:95978576-95978595 None:intergenic 35.0%
ATGTGCAGAGACGTATATAT+AGG + Chr1:95980029-95980048 MsG0180005770.01.T01:intron 35.0%
CACTTATGTATGAAGCTGTT+GGG + Chr1:95981137-95981156 MsG0180005770.01.T01:CDS 35.0%
CCTATAAATCTTGCAACAAC+AGG + Chr1:95980112-95980131 MsG0180005770.01.T01:intron 35.0%
CCTGTTGTTGCAAGATTTAT+AGG - Chr1:95980115-95980134 None:intergenic 35.0%
CTATGATGACAACTTTACAG+TGG - Chr1:95979819-95979838 None:intergenic 35.0%
GAAGACAGATTAATGAGGTT+GGG - Chr1:95979117-95979136 None:intergenic 35.0%
GAATAAGAGTAAGGTTGGAA+TGG + Chr1:95979935-95979954 MsG0180005770.01.T01:intron 35.0%
GACATACATGATCTTTGGTT+GGG - Chr1:95980945-95980964 None:intergenic 35.0%
GGAAAAATTCCCAACATTTG+CGG + Chr1:95980394-95980413 MsG0180005770.01.T01:intron 35.0%
GGATCAAATAGTAATTGCTG+AGG + Chr1:95978723-95978742 MsG0180005770.01.T01:intron 35.0%
GTTAGGAAGACAGATTAATG+AGG - Chr1:95979122-95979141 None:intergenic 35.0%
GTTTATTAAACTTTCTGCCG+CGG - Chr1:95978678-95978697 None:intergenic 35.0%
TAAGGATATATATGCAGTGC+AGG - Chr1:95980672-95980691 None:intergenic 35.0%
TATGCATTGTTGGAGAGTTT+GGG - Chr1:95977698-95977717 None:intergenic 35.0%
TCAATATGCCAAAGTTCAAG+TGG - Chr1:95979791-95979810 None:intergenic 35.0%
TCACTTATGTATGAAGCTGT+TGG + Chr1:95981136-95981155 MsG0180005770.01.T01:CDS 35.0%
TGACATACATGATCTTTGGT+TGG - Chr1:95980946-95980965 None:intergenic 35.0%
TGTTCATATGAACGAAGATG+TGG + Chr1:95979470-95979489 MsG0180005770.01.T01:intron 35.0%
TTATGCATTGTTGGAGAGTT+TGG - Chr1:95977699-95977718 None:intergenic 35.0%
! AACCACATACCAACTTTTCT+AGG + Chr1:95978564-95978583 MsG0180005770.01.T01:intron 35.0%
! ACCACATACCAACTTTTCTA+GGG + Chr1:95978565-95978584 MsG0180005770.01.T01:intron 35.0%
! ATGGTGGGAGAAAATTTGAT+GGG + Chr1:95980350-95980369 MsG0180005770.01.T01:intron 35.0%
! GAATTTTTCCACACTTGACT+TGG - Chr1:95980384-95980403 None:intergenic 35.0%
!! ATTTTTCCACACTTGACTTG+GGG - Chr1:95980382-95980401 None:intergenic 35.0%
!!! CATGGCTTGTTTTTTAGCTT+AGG - Chr1:95977728-95977747 None:intergenic 35.0%
!!! CTTGTTTTTTAGCTTAGGGT+AGG - Chr1:95977723-95977742 None:intergenic 35.0%
AAAATGTTAACTAGTGCCCC+CGG + Chr1:95979509-95979528 MsG0180005770.01.T01:intron 40.0%
AAATCAGGATTCCCAAGAAG+TGG - Chr1:95980446-95980465 None:intergenic 40.0%
AACGAAGATGTGGTCCATTA+TGG + Chr1:95979480-95979499 MsG0180005770.01.T01:intron 40.0%
AACTGGTTAACAAGACCCTA+TGG + Chr1:95979663-95979682 MsG0180005770.01.T01:CDS 40.0%
AAGTGGCAATTCTGTCAACT+TGG + Chr1:95981208-95981227 MsG0180005770.01.T01:CDS 40.0%
AATCAGGATTCCCAAGAAGT+GGG - Chr1:95980445-95980464 None:intergenic 40.0%
AATCCAGTAAATGGAGCAGT+TGG + Chr1:95981169-95981188 MsG0180005770.01.T01:CDS 40.0%
ACAGTCAGAATTTGGATACG+TGG - Chr1:95979705-95979724 None:intergenic 40.0%
AGCAAACAAGCATTCGTTGT+TGG - Chr1:95980218-95980237 None:intergenic 40.0%
AGGGTAGGATTATGCATTGT+TGG - Chr1:95977708-95977727 None:intergenic 40.0%
AGGTGTTTGATCAGCTTCAT+TGG + Chr1:95978806-95978825 MsG0180005770.01.T01:intron 40.0%
ATACATGATCTTTGGTTGGG+AGG - Chr1:95980942-95980961 None:intergenic 40.0%
ATAGAACAGTGACAAGATGC+AGG + Chr1:95978387-95978406 MsG0180005770.01.T01:intron 40.0%
ATCCAGTAAATGGAGCAGTT+GGG + Chr1:95981170-95981189 MsG0180005770.01.T01:CDS 40.0%
ATGCATTGTTGGAGAGTTTG+GGG - Chr1:95977697-95977716 None:intergenic 40.0%
CAAAGCACAAAACAGTGTTG+CGG + Chr1:95977781-95977800 MsG0180005770.01.T01:intron 40.0%
CAGCTTCATACATAAGTGAC+TGG - Chr1:95981135-95981154 None:intergenic 40.0%
CAGGTGAAAGAAAAGACATG+AGG - Chr1:95980917-95980936 None:intergenic 40.0%
GACGAGAATAAGAGTAAGGT+TGG + Chr1:95979930-95979949 MsG0180005770.01.T01:intron 40.0%
GATGTGGTCCATTATGGATA+AGG + Chr1:95979486-95979505 MsG0180005770.01.T01:intron 40.0%
GCATCTTGTCACTGTTCTAT+TGG - Chr1:95978387-95978406 None:intergenic 40.0%
GGAAGACAGATTAATGAGGT+TGG - Chr1:95979118-95979137 None:intergenic 40.0%
GTCTTTGTCACCAAGTTCTT+CGG + Chr1:95980882-95980901 MsG0180005770.01.T01:intron 40.0%
TGAAAGAAAAGACATGAGGG+TGG - Chr1:95980913-95980932 None:intergenic 40.0%
TGCATCCCTTTCTCAAAACT+CGG - Chr1:95980794-95980813 None:intergenic 40.0%
TGGAGAATGGGATTCCATTT+AGG - Chr1:95979416-95979435 None:intergenic 40.0%
TGGTGGGAGAAAATTTGATG+GGG + Chr1:95980351-95980370 MsG0180005770.01.T01:intron 40.0%
TGTCTTCCTAACGCATTGAA+GGG + Chr1:95979130-95979149 MsG0180005770.01.T01:intron 40.0%
TTAAAGGTGACTCTACCAAG+TGG - Chr1:95979336-95979355 None:intergenic 40.0%
! TGTTGGATTTGAAGTGTGAG+TGG + Chr1:95977583-95977602 MsG0180005770.01.T01:intron 40.0%
!! ACATACCAACTTTTCTAGGG+TGG + Chr1:95978568-95978587 MsG0180005770.01.T01:intron 40.0%
!! ACCCTAGAAAAGTTGGTATG+TGG - Chr1:95978569-95978588 None:intergenic 40.0%
!! GATGGTGGGAGAAAATTTGA+TGG + Chr1:95980349-95980368 MsG0180005770.01.T01:intron 40.0%
AAATGTTAACTAGTGCCCCC+GGG + Chr1:95979510-95979529 MsG0180005770.01.T01:intron 45.0%
AGGTTACTACTCCTAGTCCT+AGG + Chr1:95978633-95978652 MsG0180005770.01.T01:intron 45.0%
ATCTCCCTCTCTTGTGATCT+TGG + Chr1:95977640-95977659 MsG0180005770.01.T01:intron 45.0%
ATGCTCCAAGATCACAAGAG+AGG - Chr1:95977648-95977667 None:intergenic 45.0%
ATTGGGTAGGGTCATGTAGT+GGG - Chr1:95980471-95980490 None:intergenic 45.0%
CCACACCTAACAACTGATCA+GGG - Chr1:95981279-95981298 None:intergenic 45.0%
CTGTCTTCCTAACGCATTGA+AGG + Chr1:95979129-95979148 MsG0180005770.01.T01:intron 45.0%
GACACATGAGTCTGAGGAAT+CGG + Chr1:95981357-95981376 MsG0180005770.01.T01:CDS 45.0%
GATACAAAACCCACAAGCAC+AGG + Chr1:95980848-95980867 MsG0180005770.01.T01:intron 45.0%
GGTGGGAGAAAATTTGATGG+GGG + Chr1:95980352-95980371 MsG0180005770.01.T01:intron 45.0%
GGTTACTACTCCTAGTCCTA+GGG + Chr1:95978634-95978653 MsG0180005770.01.T01:intron 45.0%
TAACAACTGATCAGGGAGTG+CGG - Chr1:95981272-95981291 None:intergenic 45.0%
TAGTTTCCTTAACCAGTGCC+CGG - Chr1:95979531-95979550 None:intergenic 45.0%
TATTGGGTAGGGTCATGTAG+TGG - Chr1:95980472-95980491 None:intergenic 45.0%
TCAGGTTCTCCGCAAATGTT+GGG - Chr1:95980406-95980425 None:intergenic 45.0%
TCCACACCTAACAACTGATC+AGG - Chr1:95981280-95981299 None:intergenic 45.0%
TCGTGGAATCGCACAATTCT+AGG - Chr1:95977809-95977828 None:intergenic 45.0%
TGCTACGAGATTGTCTAACG+TGG + Chr1:95980826-95980845 MsG0180005770.01.T01:intron 45.0%
TGCTCCAAGATCACAAGAGA+GGG - Chr1:95977647-95977666 None:intergenic 45.0%
TGTTTCCTTAACTAGTGCCC+CGG + Chr1:95979637-95979656 MsG0180005770.01.T01:intron 45.0%
TTCTGTTCTACTTCCCAACG+TGG - Chr1:95981389-95981408 None:intergenic 45.0%
TTTGAGAAAGGGATGCAGTG+AGG + Chr1:95980797-95980816 MsG0180005770.01.T01:intron 45.0%
! GAACTTGGTGACAAAGACAG+TGG - Chr1:95980880-95980899 None:intergenic 45.0%
! GGTGAAAAGTAGGAGTGTGT+GGG - Chr1:95980583-95980602 None:intergenic 45.0%
! TGGTGAAAAGTAGGAGTGTG+TGG - Chr1:95980584-95980603 None:intergenic 45.0%
!! GAACAAGTGCTGAGAGGTAA+CGG + Chr1:95981235-95981254 MsG0180005770.01.T01:CDS 45.0%
!!! GGTTGCCGAGTTTTGAGAAA+GGG + Chr1:95980786-95980805 MsG0180005770.01.T01:intron 45.0%
!!! TTTTTTTTGTATATTTAAAT+TGG - Chr1:95979298-95979317 None:intergenic 5.0%
AGCCCAACTGCTCCATTTAC+TGG - Chr1:95981175-95981194 None:intergenic 50.0%
AGTTTCCTTAACCAGTGCCC+GGG - Chr1:95979530-95979549 None:intergenic 50.0%
ATGGAGCAGTTGGGCTGTTA+TGG + Chr1:95981179-95981198 MsG0180005770.01.T01:CDS 50.0%
CAAAGTTCAAGTGGAGCGTG+AGG - Chr1:95979782-95979801 None:intergenic 50.0%
CCAAGCAGAAAAGCAACGTG+CGG + Chr1:95981333-95981352 MsG0180005770.01.T01:CDS 50.0%
CCCTGATCAGTTGTTAGGTG+TGG + Chr1:95981276-95981295 MsG0180005770.01.T01:CDS 50.0%
CTCACGCTCCACTTGAACTT+TGG + Chr1:95979780-95979799 MsG0180005770.01.T01:intron 50.0%
CTCAGGTTCTCCGCAAATGT+TGG - Chr1:95980407-95980426 None:intergenic 50.0%
CTGTGTCCCTTCAATGCGTT+AGG - Chr1:95979139-95979158 None:intergenic 50.0%
GAAAAGACATGAGGGTGGCA+CGG - Chr1:95980908-95980927 None:intergenic 50.0%
GATCTTTGGTTGGGAGGAAC+AGG - Chr1:95980936-95980955 None:intergenic 50.0%
GCACTCCCTGATCAGTTGTT+AGG + Chr1:95981271-95981290 MsG0180005770.01.T01:CDS 50.0%
GTTTCCTTAACTAGTGCCCC+GGG + Chr1:95979638-95979657 MsG0180005770.01.T01:intron 50.0%
TCTTGTTAACCAGTTGCCCC+GGG - Chr1:95979658-95979677 None:intergenic 50.0%
TGGTAGTCAAGATCGCGAGT+TGG - Chr1:95977833-95977852 None:intergenic 50.0%
TTGGGTAGGGTCATGTAGTG+GGG - Chr1:95980470-95980489 None:intergenic 50.0%
TTTCCTTAACTAGTGCCCCG+GGG + Chr1:95979639-95979658 MsG0180005770.01.T01:intron 50.0%
! GGTATGTGGTTAATGGGTGC+CGG - Chr1:95978555-95978574 None:intergenic 50.0%
! GTCATGTAGTGGGGCAAATC+AGG - Chr1:95980461-95980480 None:intergenic 50.0%
! GTCTTGTTAACCAGTTGCCC+CGG - Chr1:95979659-95979678 None:intergenic 50.0%
! GTTGGAGAGTTTGGGGTACA+CGG - Chr1:95977690-95977709 None:intergenic 50.0%
!! GGCTGTTATGGACTGGAAAG+TGG + Chr1:95981191-95981210 MsG0180005770.01.T01:CDS 50.0%
!! GGTGTTGAACAAGTGCTGAG+AGG + Chr1:95981229-95981248 MsG0180005770.01.T01:CDS 50.0%
!!! CGGTTGCCGAGTTTTGAGAA+AGG + Chr1:95980785-95980804 MsG0180005770.01.T01:intron 50.0%
CACGTAACGTAACTGTGCCG+CGG + Chr1:95978658-95978677 MsG0180005770.01.T01:intron 55.0%
CAGTTACGTTACGTGGCCCT+AGG - Chr1:95978653-95978672 None:intergenic 55.0%
CTTGTTAACCAGTTGCCCCG+GGG - Chr1:95979657-95979676 None:intergenic 55.0%
GAATCGGAGTCGTCCACGTT+GGG + Chr1:95981373-95981392 MsG0180005770.01.T01:CDS 55.0%
GCAGTTGGGCTGTTATGGAC+TGG + Chr1:95981184-95981203 MsG0180005770.01.T01:CDS 55.0%
GGAGATCCCCAAGTCAAGTG+TGG + Chr1:95980373-95980392 MsG0180005770.01.T01:intron 55.0%
GGTGGGATTGTGTGTGTGCA+TGG - Chr1:95977746-95977765 None:intergenic 55.0%
GTTTCCTTAACCAGTGCCCG+GGG - Chr1:95979529-95979548 None:intergenic 55.0%
TCCCAAGAAGTGGGTGCTCA+GGG - Chr1:95980436-95980455 None:intergenic 55.0%
TGCGGAGACACATGAGTCTG+AGG + Chr1:95981351-95981370 MsG0180005770.01.T01:CDS 55.0%
TTCCCAAGAAGTGGGTGCTC+AGG - Chr1:95980437-95980456 None:intergenic 55.0%
TTTCCTTAACCAGTGCCCGG+GGG - Chr1:95979528-95979547 None:intergenic 55.0%
! CAAGATCGCGAGTTGGATCG+TGG - Chr1:95977826-95977845 None:intergenic 55.0%
! TGGCGTTAGCCTGTGCTTGT+GGG - Chr1:95980860-95980879 None:intergenic 55.0%
!! CAAGTGCTGAGAGGTAACGG+CGG + Chr1:95981238-95981257 MsG0180005770.01.T01:CDS 55.0%
!! GTGGTTAATGGGTGCCGGAT+TGG - Chr1:95978550-95978569 None:intergenic 55.0%
!!! CCGCACGTTGCTTTTCTGCT+TGG - Chr1:95981336-95981355 None:intergenic 55.0%
AGCCCTGAGCACCCACTTCT+TGG + Chr1:95980431-95980450 MsG0180005770.01.T01:intron 60.0%
CGCGGCACAGTTACGTTACG+TGG - Chr1:95978660-95978679 None:intergenic 60.0%
CGTTACGTGGCCCTAGGACT+AGG - Chr1:95978647-95978666 None:intergenic 60.0%
GCCCTGAGCACCCACTTCTT+GGG + Chr1:95980432-95980451 MsG0180005770.01.T01:intron 60.0%
GGAATCGGAGTCGTCCACGT+TGG + Chr1:95981372-95981391 MsG0180005770.01.T01:CDS 60.0%
! GTGGCGTTAGCCTGTGCTTG+TGG - Chr1:95980861-95980880 None:intergenic 60.0%
! TTGCCCCGGGGCACTAGTTA+AGG - Chr1:95979645-95979664 None:intergenic 60.0%
AACTAGTGCCCCGGGGCAAC+TGG + Chr1:95979646-95979665 MsG0180005770.01.T01:intron 65.0%
GGTGCTCAGGGCTGCAACTC+AGG - Chr1:95980424-95980443 None:intergenic 65.0%
GGTGGCACGGCCGAAGAACT+TGG - Chr1:95980895-95980914 None:intergenic 65.0%
TAACTAGTGCCCCCGGGCAC+TGG + Chr1:95979516-95979535 MsG0180005770.01.T01:intron 65.0%
!! GTGCCCCCGGGCACTGGTTA+AGG + Chr1:95979522-95979541 MsG0180005770.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 95977554 95981480 95977554 ID=MsG0180005770.01;Name=MsG0180005770.01
Chr1 mRNA 95977554 95981480 95977554 ID=MsG0180005770.01.T01;Parent=MsG0180005770.01;Name=MsG0180005770.01.T01;_AED=0.50;_eAED=0.62;_QI=0|0|0|1|1|1|3|0|156
Chr1 exon 95977554 95977597 95977554 ID=MsG0180005770.01.T01:exon:16125;Parent=MsG0180005770.01.T01
Chr1 exon 95979652 95979722 95979652 ID=MsG0180005770.01.T01:exon:16126;Parent=MsG0180005770.01.T01
Chr1 exon 95981125 95981480 95981125 ID=MsG0180005770.01.T01:exon:16127;Parent=MsG0180005770.01.T01
Chr1 CDS 95977554 95977597 95977554 ID=MsG0180005770.01.T01:cds;Parent=MsG0180005770.01.T01
Chr1 CDS 95979652 95979722 95979652 ID=MsG0180005770.01.T01:cds;Parent=MsG0180005770.01.T01
Chr1 CDS 95981125 95981480 95981125 ID=MsG0180005770.01.T01:cds;Parent=MsG0180005770.01.T01
Gene Sequence

>MsG0180005770.01.T01

ATGCGCGGCGCAAATGAGTCATTTAAGCATGTTGGATTTGAAGTTGCCCCGGGGCAACTGGTTAACAAGACCCTATGGATAAATATCGCAATTGCCACGTATCCAAATTCTGACTGTTTGTTCCAGTCACTTATGTATGAAGCTGTTGGGAGAACTATAAATCCAGTAAATGGAGCAGTTGGGCTGTTATGGACTGGAAAGTGGCAATTCTGTCAACTTGGTGTTGAACAAGTGCTGAGAGGTAACGGCGGCGCATTGACCGCACTCCCTGATCAGTTGTTAGGTGTGGATCATGAGAGTAATTCAATTCATCATCAATATCACCAAGCAGAAAAGCAACGTGCGGAGACACATGAGTCTGAGGAATCGGAGTCGTCCACGTTGGGAAGTAGAACAGAAACAGAAGATAGTTCTTATGTTTATGCTAATTCTCAAACTCAAAGGAACCAACTCCTTACACTATTCTTATGA
Protein sequence

>MsG0180005770.01.T01

MRGANESFKHVGFEVAPGQLVNKTLWINIAIATYPNSDCLFQSLMYEAVGRTINPVNGAVGLLWTGKWQFCQLGVEQVLRGNGGALTALPDQLLGVDHESNSIHHQYHQAEKQRAETHESEESESSTLGSRTETEDSSYVYANSQTQRNQLLTLFL*