AlfalfaGEDB | MsG0180006113.01

M. sativa cultivar ZhongmuNo.1 / MsG0180006113.01

KEGG: -
GO: GO:0000982 GO:0000987 GO:0003677 GO:0045944 GO:0046983
PFAM: PF00319

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	bit score
MsG0180006113.01.T01	KEH44429.1	81.638	354	16	3	1	312	1	347	554
MsG0180006113.01.T02	KEH44429.1	89.266	354	21	2	1	344	1	347	627
MsG0180006113.01.T03	KEH44429.1	89.266	354	19	2	1	342	1	347	629
MsG0180006113.01.T04	KEH44429.1	92.373	354	20	1	1	354	1	347	660

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180006113.01.T01	Q9FIM0	30.38	158	105	3	6	160	6	161	2.76E-17	84
MsG0180006113.01.T02	Q9FIM0	30.38	158	105	3	6	160	6	161	4.35E-17	83.6
MsG0180006113.01.T03	Q9FIM0	30.38	158	105	3	6	160	6	161	4.24E-17	83.6
MsG0180006113.01.T04	Q9FIM0	30.38	158	105	3	6	160	6	161	4.52E-17	84

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	bit score
MsG0180006113.01.T01	A0A072VR19	81.638	354	16	3	1	312	1	347	554
MsG0180006113.01.T02	A0A072VR19	89.266	354	21	2	1	344	1	347	627
MsG0180006113.01.T03	A0A072VR19	89.266	354	19	2	1	342	1	347	629
MsG0180006113.01.T04	A0A072VR19	92.373	354	20	1	1	354	1	347	660

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TFs/TRs:

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Gene ID	Type	Classification
MsG0180006113.01.T01	TF	MADS-M-type
MsG0180006113.01.T03	TF	MADS-M-type

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180006113.01.T01	MTR_1g114730	81.638	354	16	3	1	312	1	347	0.0	554
MsG0180006113.01.T01	MTR_6g018920	69.364	346	72	3	1	312	1	346	1.14e-173	484
MsG0180006113.01.T01	MTR_6g005440	62.222	315	116	2	1	312	1	315	1.02e-138	395
MsG0180006113.01.T01	MTR_6g005450	59.599	349	102	6	1	312	1	347	4.22e-134	384
MsG0180006113.01.T01	MTR_4g107170	40.741	162	93	1	1	162	1	159	4.55e-37	133
MsG0180006113.01.T01	MTR_2g049610	43.590	156	82	2	1	156	1	150	1.62e-34	126
MsG0180006113.01.T01	MTR_4g045977	35.602	191	118	3	1	190	1	187	5.94e-32	123
MsG0180006113.01.T01	MTR_2g049530	38.125	160	94	2	3	161	5	160	3.74e-29	110
MsG0180006113.01.T01	MTR_2g049630	37.888	161	95	2	2	161	4	160	3.78e-27	109
MsG0180006113.01.T01	MTR_1g115290	32.800	125	83	1	44	168	8	131	4.84e-20	85.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180006113.01.T01	AT5G55690	39.310	145	80	2	1	144	1	138	1.10e-25	103
MsG0180006113.01.T01	AT5G55690	39.310	145	80	2	1	144	1	138	1.10e-25	103
MsG0180006113.01.T01	AT5G58890	30.380	158	105	3	6	160	6	161	2.81e-18	84.0
MsG0180006113.01.T01	AT3G18650	34.815	135	74	5	16	144	31	157	3.74e-15	75.9
MsG0180006113.01.T01	AT1G22590	29.677	155	98	3	1	150	1	149	5.99e-13	66.2
MsG0180006113.01.T01	AT1G65330	33.333	111	67	4	3	112	2	106	6.25e-13	68.2
MsG0180006113.01.T01	AT1G65300	33.636	110	64	4	5	112	4	106	6.55e-13	68.2
MsG0180006113.01.T01	AT3G05860	37.662	77	42	2	1	77	1	71	8.98e-13	66.6
MsG0180006113.01.T01	AT3G05860	37.662	77	42	2	1	77	1	71	1.26e-12	67.0
MsG0180006113.01.T01	AT5G23260	25.243	206	123	6	1	192	1	189	1.43e-12	66.6

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Find 78 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCCTCGACCTACAAAAT+TGG	0.237707	1:+100283802	None:intergenic
AACATCAACATCACAAAGAA+TGG	0.436469	1:+100283844	None:intergenic
AAGGAAGGATGGAGCTGAAA+AGG	0.426747	1:-100283087	MsG0180006113.01.T04:CDS
AATAGCTCATGAATCACACA+AGG	0.705760	1:-100283465	MsG0180006113.01.T04:CDS
AATCCTCGACCTACAAAATT+GGG	0.455489	1:+100283803	None:intergenic
AATCTCTCAAATCTACAACA+TGG	0.661149	1:-100282978	MsG0180006113.01.T04:CDS
AATGAATCTTTCAATTCTCT+TGG	0.359377	1:-100283587	MsG0180006113.01.T04:CDS
AATGCTAATGATACCTACAA+TGG	0.561788	1:-100283155	MsG0180006113.01.T04:CDS
ACAAGAATATTGATGTTAAT+GGG	0.303821	1:-100283199	MsG0180006113.01.T04:CDS
ACATTTCAAAAGAGAAAGGA+AGG	0.560555	1:-100283893	MsG0180006113.01.T04:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACATCAACATCACAAAGAA+TGG	+	Chr1:100283017-100283036	None:intergenic	30.0%
AATCTCTCAAATCTACAACA+TGG	-	Chr1:100283880-100283899	MsG0180006113.01.T04:CDS	30.0%
AATGCTAATGATACCTACAA+TGG	-	Chr1:100283703-100283722	MsG0180006113.01.T04:CDS	30.0%
ACATTTCAAAAGAGAAAGGA+AGG	-	Chr1:100282965-100282984	MsG0180006113.01.T04:CDS	30.0%
ATCATTAGCATTAACCTGAT+TGG	+	Chr1:100283695-100283714	None:intergenic	30.0%
ATGAGATTGAAGTAGTAACT+TGG	+	Chr1:100283457-100283476	None:intergenic	30.0%
ATGCTAATGATACCTACAAT+GGG	-	Chr1:100283704-100283723	MsG0180006113.01.T04:CDS	30.0%
ATTAGTGATAAAAACCCCTT+AGG	-	Chr1:100283523-100283542	MsG0180006113.01.T04:CDS	30.0%
CAAAATCAATATGGAAAGGA+AGG	-	Chr1:100283756-100283775	MsG0180006113.01.T04:CDS	30.0%
CAACATGGATTTCAACTTAA+TGG	-	Chr1:100283895-100283914	MsG0180006113.01.T04:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	100282908	100283972	100282908	ID=MsG0180006113.01;Name=MsG0180006113.01
Chr1	mRNA	100282908	100283972	100282908	ID=MsG0180006113.01.T01;Parent=MsG0180006113.01;Name=MsG0180006113.01.T01;_AED=0.06;_eAED=0.06;_QI=0\|0\|0\|1\|0\|0\|3\|0\|312
Chr1	exon	100282908	100283121	100282908	ID=MsG0180006113.01.T01:exon:38182;Parent=MsG0180006113.01.T01
Chr1	exon	100283218	100283465	100283218	ID=MsG0180006113.01.T01:exon:38181;Parent=MsG0180006113.01.T01
Chr1	exon	100283496	100283972	100283496	ID=MsG0180006113.01.T01:exon:38180;Parent=MsG0180006113.01.T01
Chr1	CDS	100283496	100283972	100283496	ID=MsG0180006113.01.T01:cds;Parent=MsG0180006113.01.T01
Chr1	CDS	100283218	100283465	100283218	ID=MsG0180006113.01.T01:cds;Parent=MsG0180006113.01.T01
Chr1	CDS	100282908	100283121	100282908	ID=MsG0180006113.01.T01:cds;Parent=MsG0180006113.01.T01
Chr1	mRNA	100282908	100283972	100282908	ID=MsG0180006113.01.T02;Parent=MsG0180006113.01;Name=MsG0180006113.01.T02;_AED=0.02;_eAED=0.02;_QI=0\|0\|0\|1\|0\|0\|2\|0\|344
Chr1	exon	100282908	100283122	100282908	ID=MsG0180006113.01.T02:exon:38184;Parent=MsG0180006113.01.T02
Chr1	exon	100283153	100283972	100283153	ID=MsG0180006113.01.T02:exon:38183;Parent=MsG0180006113.01.T02
Chr1	CDS	100283153	100283972	100283153	ID=MsG0180006113.01.T02:cds;Parent=MsG0180006113.01.T02
Chr1	CDS	100282908	100283122	100282908	ID=MsG0180006113.01.T02:cds;Parent=MsG0180006113.01.T02
Chr1	mRNA	100282908	100283972	100282908	ID=MsG0180006113.01.T03;Parent=MsG0180006113.01;Name=MsG0180006113.01.T03;_AED=0.02;_eAED=0.02;_QI=0\|0\|0\|1\|0\|0\|2\|0\|342
Chr1	exon	100282908	100283144	100282908	ID=MsG0180006113.01.T03:exon:38186;Parent=MsG0180006113.01.T03
Chr1	exon	100283181	100283972	100283181	ID=MsG0180006113.01.T03:exon:38185;Parent=MsG0180006113.01.T03
Chr1	CDS	100283181	100283972	100283181	ID=MsG0180006113.01.T03:cds;Parent=MsG0180006113.01.T03
Chr1	CDS	100282908	100283144	100282908	ID=MsG0180006113.01.T03:cds;Parent=MsG0180006113.01.T03
Chr1	mRNA	100282908	100283972	100282908	ID=MsG0180006113.01.T04;Parent=MsG0180006113.01;Name=MsG0180006113.01.T04;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|354
Chr1	exon	100282908	100283972	100282908	ID=MsG0180006113.01.T04:exon:38187;Parent=MsG0180006113.01.T04
Chr1	CDS	100282908	100283972	100282908	ID=MsG0180006113.01.T04:cds;Parent=MsG0180006113.01.T04

Gene Sequence

>MsG0180006113.01.T04

ATGGGTCGTGGAAGAATATCTATGGAACTCATCCAAAAGGAGAAATCACGGAAGTCAACATTTCAAAAGAGAAAGGAAGGATTAATGAAAAAAGTGAATGAATTTTCCATTCTTTGTGATGTTGATGTTTGTGTAGTTCTCTATGCTCCCAATTTTGTAGGTCGAGGATTTGCTGAACCTGAAACATGGCCTAAGGACAAAAGAGAGGTGCATAGAATCCTTCAAAAGTACTATAACACAACAAATGATAGGCGTCCTAAGATCTATGATGTTCAAGAGTATTTTAAGGAAAGGATGAGAAAACTTGAATTTGAGATTTCCAAAGTGCGTAAAGAGAAACTAAAGATGATGTATCCAACTTGGAATGAATCTTTCAATTCTCTTGGAGCGGAACAACTGATATTATTTGCTAGCAAACTGGAAGCTAAGCTTGATGCTTGTAATCAAAAGAAGCATATGCTAAAAGAAGATCATAAGGGAAAAACAATAGCTCATGAATCACACAAGGTAGATACACTTATCAGTACTCCTTCCTTGACCGCAAATCCAAGTTACTACTTCAATCTCATGCAAAATTCCATGTCTCAAGCACAAACATATCCTCCAATGATGAATATTAGTGATAAAAACCCCTTAGGGTTTTGGCCACTTCAACCGGGACAAAGCTCTCAACCTTCTTCTTTGATTTCCAATATACAAAGCTCGCACCATGTGGAGTCTGAGGAGGGTAGATTTGTGCAGTCTTACCCCTACAAGAATATTGATGTTAATGGGGCTAATTGGGCCAATCAGGTTAATGCTAATGATACCTACAATGGGGGTTATCAGGACCGATGCTTCGGGCTTCCCAAAATCAATATGGAAAGGAAGGATGGAGCTGAAAAGGATGAAAACTCGTCACCATACTATTACAATGGAAATGCAATGGCAATGCAATCAAATCCTATTGCCTTGCACACTCTCCCTTTTCAGAATCTCTCAAATCTACAACATGGATTTCAACTTAATGGATTCAATGCTAAGGATATTCTGCAAGCTCATATGTTCATGAATGGAAGGAAGTAG

>MsG0180006113.01.T02

ATGGGTCGTGGAAGAATATCTATGGAACTCATCCAAAAGGAGAAATCACGGAAGTCAACATTTCAAAAGAGAAAGGAAGGATTAATGAAAAAAGTGAATGAATTTTCCATTCTTTGTGATGTTGATGTTTGTGTAGTTCTCTATGCTCCCAATTTTGTAGGTCGAGGATTTGCTGAACCTGAAACATGGCCTAAGGACAAAAGAGAGGTGCATAGAATCCTTCAAAAGTACTATAACACAACAAATGATAGGCGTCCTAAGATCTATGATGTTCAAGAGTATTTTAAGGAAAGGATGAGAAAACTTGAATTTGAGATTTCCAAAGTGCGTAAAGAGAAACTAAAGATGATGTATCCAACTTGGAATGAATCTTTCAATTCTCTTGGAGCGGAACAACTGATATTATTTGCTAGCAAACTGGAAGCTAAGCTTGATGCTTGTAATCAAAAGAAGCATATGCTAAAAGAAGATCATAAGGGAAAAACAATAGCTCATGAATCACACAAGGTAGATACACTTATCAGTACTCCTTCCTTGACCGCAAATCCAAGTTACTACTTCAATCTCATGCAAAATTCCATGTCTCAAGCACAAACATATCCTCCAATGATGAATATTAGTGATAAAAACCCCTTAGGGTTTTGGCCACTTCAACCGGGACAAAGCTCTCAACCTTCTTCTTTGATTTCCAATATACAAAGCTCGCACCATGTGGAGTCTGAGGAGGGTAGATTTGTGCAGTCTTACCCCTACAAGAATATTGATGTTAATGGGGCTAATTGGGCCAATCAGGTTAATGCTAATGATACCTACAATGGGGAAATCAATATGGAAAGGAAGGATGGAGCTGAAAAGGATGAAAACTCGTCACCATACTATTACAATGGAAATGCAATGGCAATGCAATCAAATCCTATTGCCTTGCACACTCTCCCTTTTCAGAATCTCTCAAATCTACAACATGGATTTCAACTTAATGGATTCAATGCTAAGGATATTCTGCAAGCTCATATGTTCATGAATGGAAGGAAGTAG

>MsG0180006113.01.T01

ATGGGTCGTGGAAGAATATCTATGGAACTCATCCAAAAGGAGAAATCACGGAAGTCAACATTTCAAAAGAGAAAGGAAGGATTAATGAAAAAAGTGAATGAATTTTCCATTCTTTGTGATGTTGATGTTTGTGTAGTTCTCTATGCTCCCAATTTTGTAGGTCGAGGATTTGCTGAACCTGAAACATGGCCTAAGGACAAAAGAGAGGTGCATAGAATCCTTCAAAAGTACTATAACACAACAAATGATAGGCGTCCTAAGATCTATGATGTTCAAGAGTATTTTAAGGAAAGGATGAGAAAACTTGAATTTGAGATTTCCAAAGTGCGTAAAGAGAAACTAAAGATGATGTATCCAACTTGGAATGAATCTTTCAATTCTCTTGGAGCGGAACAACTGATATTATTTGCTAGCAAACTGGAAGCTAAGCTTGATGCTTGTAATCAAAAGAAGCATATGCTAAAAGAAGATCATAAGGTAGATACACTTATCAGTACTCCTTCCTTGACCGCAAATCCAAGTTACTACTTCAATCTCATGCAAAATTCCATGTCTCAAGCACAAACATATCCTCCAATGATGAATATTAGTGATAAAAACCCCTTAGGGTTTTGGCCACTTCAACCGGGACAAAGCTCTCAACCTTCTTCTTTGATTTCCAATATACAAAGCTCGCACCATGTGGAGTCTGAGGAGGGTAGATTTGTGCAGTCTTACCCCTACAAAATCAATATGGAAAGGAAGGATGGAGCTGAAAAGGATGAAAACTCGTCACCATACTATTACAATGGAAATGCAATGGCAATGCAATCAAATCCTATTGCCTTGCACACTCTCCCTTTTCAGAATCTCTCAAATCTACAACATGGATTTCAACTTAATGGATTCAATGCTAAGGATATTCTGCAAGCTCATATGTTCATGAATGGAAGGAAGTAG

>MsG0180006113.01.T03

ATGGGTCGTGGAAGAATATCTATGGAACTCATCCAAAAGGAGAAATCACGGAAGTCAACATTTCAAAAGAGAAAGGAAGGATTAATGAAAAAAGTGAATGAATTTTCCATTCTTTGTGATGTTGATGTTTGTGTAGTTCTCTATGCTCCCAATTTTGTAGGTCGAGGATTTGCTGAACCTGAAACATGGCCTAAGGACAAAAGAGAGGTGCATAGAATCCTTCAAAAGTACTATAACACAACAAATGATAGGCGTCCTAAGATCTATGATGTTCAAGAGTATTTTAAGGAAAGGATGAGAAAACTTGAATTTGAGATTTCCAAAGTGCGTAAAGAGAAACTAAAGATGATGTATCCAACTTGGAATGAATCTTTCAATTCTCTTGGAGCGGAACAACTGATATTATTTGCTAGCAAACTGGAAGCTAAGCTTGATGCTTGTAATCAAAAGAAGCATATGCTAAAAGAAGATCATAAGGGAAAAACAATAGCTCATGAATCACACAAGGTAGATACACTTATCAGTACTCCTTCCTTGACCGCAAATCCAAGTTACTACTTCAATCTCATGCAAAATTCCATGTCTCAAGCACAAACATATCCTCCAATGATGAATATTAGTGATAAAAACCCCTTAGGGTTTTGGCCACTTCAACCGGGACAAAGCTCTCAACCTTCTTCTTTGATTTCCAATATACAAAGCTCGCACCATGTGGAGTCTGAGGAGGGTAGATTTGTGCAGTCTTACCCCTACAAGAATATTGATGTTAATGGGGCTAATTGGGCCAATCAGGACCGATGCTTCGGGCTTCCCAAAATCAATATGGAAAGGAAGGATGGAGCTGAAAAGGATGAAAACTCGTCACCATACTATTACAATGGAAATGCAATGGCAATGCAATCAAATCCTATTGCCTTGCACACTCTCCCTTTTCAGAATCTCTCAAATCTACAACATGGATTTCAACTTAATGGATTCAATGCTAAGGATATTCTGCAAGCTCATATGTTCATGAATGGAAGGAAGTAG

Protein sequence

>MsG0180006113.01.T04

MGRGRISMELIQKEKSRKSTFQKRKEGLMKKVNEFSILCDVDVCVVLYAPNFVGRGFAEPETWPKDKREVHRILQKYYNTTNDRRPKIYDVQEYFKERMRKLEFEISKVRKEKLKMMYPTWNESFNSLGAEQLILFASKLEAKLDACNQKKHMLKEDHKGKTIAHESHKVDTLISTPSLTANPSYYFNLMQNSMSQAQTYPPMMNISDKNPLGFWPLQPGQSSQPSSLISNIQSSHHVESEEGRFVQSYPYKNIDVNGANWANQVNANDTYNGGYQDRCFGLPKINMERKDGAEKDENSSPYYYNGNAMAMQSNPIALHTLPFQNLSNLQHGFQLNGFNAKDILQAHMFMNGRK*

>MsG0180006113.01.T02

MGRGRISMELIQKEKSRKSTFQKRKEGLMKKVNEFSILCDVDVCVVLYAPNFVGRGFAEPETWPKDKREVHRILQKYYNTTNDRRPKIYDVQEYFKERMRKLEFEISKVRKEKLKMMYPTWNESFNSLGAEQLILFASKLEAKLDACNQKKHMLKEDHKGKTIAHESHKVDTLISTPSLTANPSYYFNLMQNSMSQAQTYPPMMNISDKNPLGFWPLQPGQSSQPSSLISNIQSSHHVESEEGRFVQSYPYKNIDVNGANWANQVNANDTYNGEINMERKDGAEKDENSSPYYYNGNAMAMQSNPIALHTLPFQNLSNLQHGFQLNGFNAKDILQAHMFMNGRK*

>MsG0180006113.01.T01

MGRGRISMELIQKEKSRKSTFQKRKEGLMKKVNEFSILCDVDVCVVLYAPNFVGRGFAEPETWPKDKREVHRILQKYYNTTNDRRPKIYDVQEYFKERMRKLEFEISKVRKEKLKMMYPTWNESFNSLGAEQLILFASKLEAKLDACNQKKHMLKEDHKVDTLISTPSLTANPSYYFNLMQNSMSQAQTYPPMMNISDKNPLGFWPLQPGQSSQPSSLISNIQSSHHVESEEGRFVQSYPYKINMERKDGAEKDENSSPYYYNGNAMAMQSNPIALHTLPFQNLSNLQHGFQLNGFNAKDILQAHMFMNGRK*

>MsG0180006113.01.T03

MGRGRISMELIQKEKSRKSTFQKRKEGLMKKVNEFSILCDVDVCVVLYAPNFVGRGFAEPETWPKDKREVHRILQKYYNTTNDRRPKIYDVQEYFKERMRKLEFEISKVRKEKLKMMYPTWNESFNSLGAEQLILFASKLEAKLDACNQKKHMLKEDHKGKTIAHESHKVDTLISTPSLTANPSYYFNLMQNSMSQAQTYPPMMNISDKNPLGFWPLQPGQSSQPSSLISNIQSSHHVESEEGRFVQSYPYKNIDVNGANWANQDRCFGLPKINMERKDGAEKDENSSPYYYNGNAMAMQSNPIALHTLPFQNLSNLQHGFQLNGFNAKDILQAHMFMNGRK*