AlfalfaGEDB | MsG0280006603.01

M. sativa cultivar ZhongmuNo.1 / MsG0280006603.01

KEGG: K09422
GO: GO:0003677
PFAM: PF00249

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006603.01.T01	QSD99628.1	86.649	367	37	9	1	362	1	360	0	584

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006603.01.T01	Q9LDR8	53.887	373	137	12	1	362	1	349	4.50E-109	325

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006603.01.T01	A0A896WEJ8	86.649	367	37	9	1	362	1	360	0.0	584

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TFs/TRs:

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Gene ID	Type	Classification
MsG0280006603.01.T01	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006603.01.T01	MTR_2g011660	78.226	372	44	10	1	362	1	345	1.02e-169	477
MsG0280006603.01.T01	MTR_4g091490	55.959	386	118	14	1	362	1	358	2.06e-119	350
MsG0280006603.01.T01	MTR_2g089620	50.538	372	128	14	1	362	1	326	6.62e-95	286
MsG0280006603.01.T01	MTR_7g011170	76.119	134	31	1	1	134	1	133	7.28e-76	237
MsG0280006603.01.T01	MTR_3g074520	75.373	134	32	1	1	134	1	133	1.97e-75	237
MsG0280006603.01.T01	MTR_5g029840	72.848	151	37	2	1	148	1	150	6.25e-75	235
MsG0280006603.01.T01	MTR_6g012690	58.763	194	75	2	1	194	1	189	1.05e-74	235
MsG0280006603.01.T01	MTR_4g478180	69.388	147	44	1	1	147	2	147	7.22e-74	233
MsG0280006603.01.T01	MTR_4g082290	77.273	132	29	1	1	132	1	131	1.29e-73	234
MsG0280006603.01.T01	MTR_4g082230	74.242	132	33	1	1	132	1	131	1.34e-69	224

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006603.01.T01	AT4G21440	53.887	373	137	12	1	362	1	349	4.58e-110	325
MsG0280006603.01.T01	AT4G05100	68.619	239	49	5	1	215	1	237	7.06e-100	298
MsG0280006603.01.T01	AT4G28110	88.462	130	14	1	1	130	1	129	1.18e-84	258
MsG0280006603.01.T01	AT5G16770	64.641	181	63	1	1	181	1	180	4.23e-80	248
MsG0280006603.01.T01	AT5G16770	64.641	181	63	1	1	181	1	180	4.23e-80	248
MsG0280006603.01.T01	AT5G16770	64.641	181	63	1	1	181	1	180	1.08e-79	248
MsG0280006603.01.T01	AT5G16770	64.641	181	63	1	1	181	1	180	1.08e-79	248
MsG0280006603.01.T01	AT5G54230	63.000	200	64	4	13	206	12	207	2.36e-79	246
MsG0280006603.01.T01	AT3G02940	77.941	136	29	1	1	136	1	135	8.22e-77	239
MsG0280006603.01.T01	AT3G02940	77.941	136	29	1	1	136	1	135	2.04e-76	238

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Find 72 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTTATGAGAGTTGTAAT+AGG	0.336516	2:-3960389	MsG0280006603.01.T01:CDS
AAATATATACCTGCATTCTT+TGG	0.219901	2:+3961527	None:intergenic
AACAACTTCCAAAATCTCCT+TGG	0.494886	2:-3960945	MsG0280006603.01.T01:CDS
AACATGGTTATGGCAATTGG+AGG	0.580738	2:-3961556	MsG0280006603.01.T01:CDS
AACGCAAGGTGTAGTGAATG+TGG	0.702936	2:+3960719	None:intergenic
AAGAATAATGGTCTCAAGAA+AGG	0.479998	2:-3961626	MsG0280006603.01.T01:CDS
AATATATACCTGCATTCTTT+GGG	0.380312	2:+3961528	None:intergenic
AATTGGACAAGATGTGAAAG+TGG	0.614871	2:+3960789	None:intergenic
ACAATTGCATCTCGATTGCC+AGG	0.496013	2:-3961131	MsG0280006603.01.T01:CDS
ACTATTGGAACACACACATT+AGG	0.422347	2:-3961085	MsG0280006603.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGAATAATGGTCTCAAGAA+AGG	-	Chr2:3960362-3960381	MsG0280006603.01.T01:CDS	30.0%
ATGAACATAGAATAGAAGAG+AGG	+	Chr2:3960991-3961010	None:intergenic	30.0%
ATTCTATGTTCATCTCTCTA+TGG	-	Chr2:3960998-3961017	MsG0280006603.01.T01:CDS	30.0%
ATTGATTGGTACGAAAATGA+TGG	-	Chr2:3961385-3961404	MsG0280006603.01.T01:CDS	30.0%
CATAGCAAAAAAAGGAATAG+AGG	+	Chr2:3960731-3960750	None:intergenic	30.0%
GTACAAGCATTATTAGCATT+AGG	+	Chr2:3961247-3961266	None:intergenic	30.0%
TAAATAGGTTTGCAAAGATG+TGG	-	Chr2:3960586-3960605	MsG0280006603.01.T01:CDS	30.0%
TACATTCAGAAACATGGTTA+TGG	-	Chr2:3960422-3960441	MsG0280006603.01.T01:CDS	30.0%
TTCTGTATTTGAGGATTACA+AGG	+	Chr2:3961172-3961191	None:intergenic	30.0%
AACAACTTCCAAAATCTCCT+TGG	-	Chr2:3961043-3961062	MsG0280006603.01.T01:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	3960338	3961672	3960338	ID=MsG0280006603.01;Name=MsG0280006603.01
Chr2	mRNA	3960338	3961672	3960338	ID=MsG0280006603.01.T01;Parent=MsG0280006603.01;Name=MsG0280006603.01.T01;_AED=0.16;_eAED=0.16;_QI=0\|0\|0\|1\|1\|1\|3\|0\|362
Chr2	exon	3961537	3961672	3961537	ID=MsG0280006603.01.T01:exon:1846;Parent=MsG0280006603.01.T01
Chr2	exon	3961288	3961417	3961288	ID=MsG0280006603.01.T01:exon:1845;Parent=MsG0280006603.01.T01
Chr2	exon	3960338	3961160	3960338	ID=MsG0280006603.01.T01:exon:1844;Parent=MsG0280006603.01.T01
Chr2	CDS	3961537	3961672	3961537	ID=MsG0280006603.01.T01:cds;Parent=MsG0280006603.01.T01
Chr2	CDS	3961288	3961417	3961288	ID=MsG0280006603.01.T01:cds;Parent=MsG0280006603.01.T01
Chr2	CDS	3960338	3961160	3960338	ID=MsG0280006603.01.T01:cds;Parent=MsG0280006603.01.T01

Gene Sequence

>MsG0280006603.01.T01

ATGGGAAGATCACCTTGTTGTGAGAAGAATAATGGTCTCAAGAAAGGACCATGGACAACTGAGGAGGATCAGAAACTCATTGATTACATTCAGAAACATGGTTATGGCAATTGGAGGACACTCCCAAAGAATGCAGGTTTGCAAAGATGTGGCAAAAGTTGTCGTCTCCGCTGGACAAACTATCTCCGACCAGATATAAAACGTGGTCGGTTTTCATTTGAAGAGGAAGAGACAATTATTCAGCTACATAGCATTCTTGGCAACAAGTGGTCTACAATTGCATCTCGATTGCCAGGAAGGACGGACAATGAAATAAAAAACTATTGGAACACACACATTAGGAAAAGGCTTTTGAGAATGGGAATTGATCCAGTGACACATAGTCCTAGACTTGACTTATTGGACCTCTCTTCTATTCTATGTTCATCTCTCTATGGTAATTCATCACCTCAAATGATGAACAACTTCCAAAATCTCCTTGGCATGCAACAACCTTTGGTGAATCCTGAGCTTCTAAAGTTAGCTTCCTCACTCTTCAGTACCTCATCACACCAACAACATCAACAAGATTATAATAATAATCAACCTTGTAATCCTCAAATACAGAACCAAGTTCCACTTTCACATCTTGTCCAATTTCAAGACCAAGTTCAACAAGTACCTAATGCTAATAATGCTTGTACAACCACATTCACTACACCTTGCGTTTCTTCTTCATTGCCAAATCAATCACATTTGTTTGAACCCAATGTGAATACATGTCCATCAAATTTCACCGACCTTAGTAATTACTATCAAAATATTGATTGGTACGAAAATGATGGATTTTCTACCTTAACAGATGATTATGTCCCTCAACTATCAAGCTATAATAATCATCATTATGGTTCTGATAATCAAAATCTTATGTATCAAACTTCAAATCAACTTCTATCAACACCATCATCTTCTAGTCCCACACCGTTGAATTCAAACTCCACGTGTATCATTGGGAGCAACACAGAAGATGAAAGAGAAAGTTATGAGAGTTGTAATAGGTTGAATTTTGAAATCCCTGCACATATTTTAGAAGCCAATGATTTTATGTAA

Protein sequence

>MsG0280006603.01.T01

MGRSPCCEKNNGLKKGPWTTEEDQKLIDYIQKHGYGNWRTLPKNAGLQRCGKSCRLRWTNYLRPDIKRGRFSFEEEETIIQLHSILGNKWSTIASRLPGRTDNEIKNYWNTHIRKRLLRMGIDPVTHSPRLDLLDLSSILCSSLYGNSSPQMMNNFQNLLGMQQPLVNPELLKLASSLFSTSSHQQHQQDYNNNQPCNPQIQNQVPLSHLVQFQDQVQQVPNANNACTTTFTTPCVSSSLPNQSHLFEPNVNTCPSNFTDLSNYYQNIDWYENDGFSTLTDDYVPQLSSYNNHHYGSDNQNLMYQTSNQLLSTPSSSSPTPLNSNSTCIIGSNTEDERESYESCNRLNFEIPAHILEANDFM*