AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006735.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006735.01.T01 MTR_2g013900 95.714 140 5 1 31 170 330 468 6.69e-96 285
MsG0280006735.01.T01 MTR_4g088750 82.143 140 24 1 31 170 369 507 4.25e-80 246
MsG0280006735.01.T01 MTR_4g088745 75.352 142 32 2 31 170 213 353 2.79e-73 224
MsG0280006735.01.T01 MTR_4g088755 75.352 142 32 2 31 170 208 348 3.03e-73 224
MsG0280006735.01.T01 MTR_3g034100 49.462 93 46 1 31 123 249 340 1.12e-29 111
MsG0280006735.01.T01 MTR_3g078000 49.541 109 54 1 31 139 224 331 1.63e-29 111
MsG0280006735.01.T01 MTR_4g132860 49.515 103 51 1 31 133 247 348 2.53e-29 110
MsG0280006735.01.T01 MTR_4g105770 48.485 99 50 1 31 129 225 322 2.86e-29 110
MsG0280006735.01.T01 MTR_8g100085 49.462 93 46 1 31 123 300 391 3.89e-29 110
MsG0280006735.01.T01 MTR_1g026160 47.423 97 50 1 31 127 307 402 8.93e-26 102
MsG0280006735.01.T01 MTR_1g113960 40.650 123 66 2 31 153 463 578 8.05e-25 100
MsG0280006735.01.T01 MTR_5g023150 47.253 91 47 1 31 121 360 449 4.23e-24 97.8
MsG0280006735.01.T01 MTR_5g023150 47.253 91 47 1 31 121 457 546 5.63e-24 97.8
MsG0280006735.01.T01 MTR_1g113960 48.913 92 46 1 31 122 463 553 8.45e-24 97.4
MsG0280006735.01.T01 MTR_7g072710 44.706 85 47 0 38 122 188 272 1.74e-22 92.0
MsG0280006735.01.T01 MTR_7g072710 44.706 85 47 0 38 122 225 309 2.03e-22 92.4
MsG0280006735.01.T01 MTR_3g114900 47.191 89 46 1 31 119 432 519 1.28e-21 90.9
MsG0280006735.01.T01 MTR_1g103270 35.714 140 79 4 10 140 236 373 4.23e-21 89.0
MsG0280006735.01.T01 MTR_6g007603 39.091 110 65 2 31 140 259 366 5.88e-21 88.2
MsG0280006735.01.T01 MTR_3g078110 35.714 126 72 3 31 156 247 363 2.79e-20 86.3
MsG0280006735.01.T01 MTR_4g106980 35.714 126 72 3 31 156 276 392 6.01e-20 85.9
MsG0280006735.01.T01 MTR_2g461180 38.614 101 61 1 31 130 1301 1401 1.18e-19 85.5
MsG0280006735.01.T01 MTR_3g465590 43.836 73 41 0 47 119 508 580 3.22e-19 84.3
MsG0280006735.01.T01 MTR_6g033130 44.186 86 48 0 45 130 1156 1241 4.64e-19 84.0
MsG0280006735.01.T01 MTR_1g099110 43.529 85 46 2 31 115 428 510 6.27e-19 83.2
MsG0280006735.01.T01 MTR_1g099110 43.529 85 46 2 31 115 445 527 6.54e-19 83.2
MsG0280006735.01.T01 MTR_4g128650 40.206 97 57 1 31 126 985 1081 2.12e-18 82.0
MsG0280006735.01.T01 MTR_5g006560 40.000 95 56 1 31 125 308 401 3.14e-18 81.3
MsG0280006735.01.T01 MTR_5g006560 40.000 95 56 1 31 125 274 367 3.29e-18 80.9
MsG0280006735.01.T01 MTR_4g116520 39.394 99 59 1 31 128 1266 1364 9.35e-18 80.1
MsG0280006735.01.T01 MTR_5g072500 38.667 75 46 0 47 121 511 585 5.47e-17 77.8
MsG0280006735.01.T01 MTR_8g442400 46.667 75 40 0 45 119 1575 1649 1.04e-16 77.0
MsG0280006735.01.T01 MTR_6g004440 34.444 90 58 1 38 127 168 256 1.45e-16 75.9
MsG0280006735.01.T01 MTR_1g050690 44.565 92 50 1 31 122 584 674 3.67e-16 75.5
MsG0280006735.01.T01 MTR_3g100090 43.038 79 45 0 44 122 832 910 5.45e-16 75.1
MsG0280006735.01.T01 MTR_1g090020 35.354 99 63 1 31 128 1074 1172 6.99e-16 74.7
MsG0280006735.01.T01 MTR_5g066490 41.250 80 47 0 43 122 824 903 4.42e-15 72.4
MsG0280006735.01.T01 MTR_3g076970 35.366 82 52 1 38 119 206 286 6.43e-15 71.2
MsG0280006735.01.T01 MTR_5g009600 38.384 99 57 3 42 138 445 541 7.47e-15 71.6
MsG0280006735.01.T01 MTR_1g016300 37.755 98 60 1 31 128 828 924 8.85e-15 71.6
MsG0280006735.01.T01 MTR_4g122160 43.902 82 45 1 31 112 625 705 1.01e-14 71.2
MsG0280006735.01.T01 MTR_4g082325 36.364 99 62 1 31 129 314 411 1.65e-14 70.5
MsG0280006735.01.T01 MTR_1g061190 40.217 92 54 1 31 122 722 812 2.46e-14 70.1
MsG0280006735.01.T01 MTR_3g104900 40.860 93 49 2 36 122 349 441 2.56e-13 67.0
MsG0280006735.01.T01 MTR_2g034020 35.052 97 62 1 31 127 631 726 2.82e-13 67.0
MsG0280006735.01.T01 MTR_8g027990 40.000 80 45 2 42 119 427 505 2.88e-13 67.0
MsG0280006735.01.T01 MTR_8g107000 34.375 96 62 1 31 126 894 988 4.52e-13 66.6
MsG0280006735.01.T01 MTR_1g081790 37.778 90 55 1 31 120 854 942 5.20e-13 66.2
MsG0280006735.01.T01 MTR_5g010730 33.333 87 57 1 31 117 588 673 8.96e-13 65.9
MsG0280006735.01.T01 MTR_5g010730 33.333 87 57 1 31 117 643 728 9.80e-13 65.5
MsG0280006735.01.T01 MTR_1g050520 35.514 107 51 3 31 120 208 313 1.34e-12 65.1
MsG0280006735.01.T01 MTR_6g079540 33.333 96 63 1 31 126 825 919 2.77e-12 64.3
MsG0280006735.01.T01 MTR_1g026160 53.846 52 23 1 31 82 452 502 3.29e-12 63.9
MsG0280006735.01.T01 MTR_1g026160 56.000 50 21 1 31 80 307 355 3.65e-12 63.5
MsG0280006735.01.T01 MTR_1g026160 53.846 52 23 1 31 82 305 355 3.65e-12 63.5
MsG0280006735.01.T01 MTR_1g026160 56.000 50 21 1 31 80 452 500 3.72e-12 63.9
MsG0280006735.01.T01 MTR_1g026160 53.846 52 23 1 31 82 307 357 3.74e-12 63.5
MsG0280006735.01.T01 MTR_1g026160 56.000 50 21 1 31 80 305 353 3.77e-12 63.5
MsG0280006735.01.T01 MTR_6g079540 33.333 96 63 1 31 126 577 671 4.61e-12 63.5
MsG0280006735.01.T01 MTR_3g073250 39.080 87 48 3 24 109 429 511 2.48e-11 61.6
MsG0280006735.01.T01 MTR_8g088590 31.522 92 60 1 42 130 356 447 7.83e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006735.01.T01 AT5G58950 69.531 128 38 1 31 158 376 502 2.08e-60 196
MsG0280006735.01.T01 AT3G46930 56.098 123 52 2 31 152 332 453 3.01e-41 144
MsG0280006735.01.T01 AT3G46930 56.098 123 52 2 31 152 367 488 4.57e-41 144
MsG0280006735.01.T01 AT1G62400 45.902 122 63 3 31 152 253 371 1.95e-30 114
MsG0280006735.01.T01 AT2G24360 45.833 96 51 1 31 126 297 391 6.93e-29 110
MsG0280006735.01.T01 AT4G31170 45.833 96 51 1 31 126 298 392 7.62e-28 107
MsG0280006735.01.T01 AT4G31170 45.833 96 51 1 31 126 298 392 7.62e-28 107
MsG0280006735.01.T01 AT4G31170 45.833 96 51 1 31 126 298 392 7.62e-28 107
MsG0280006735.01.T01 AT4G31170 45.833 96 51 1 31 126 298 392 7.62e-28 107
MsG0280006735.01.T01 AT4G31170 45.833 96 51 1 31 126 298 392 7.62e-28 107
MsG0280006735.01.T01 AT4G31170 45.833 96 51 1 31 126 298 392 7.62e-28 107
MsG0280006735.01.T01 AT4G31170 45.833 96 51 1 31 126 298 392 7.62e-28 107
MsG0280006735.01.T01 AT4G38470 44.898 98 53 1 31 128 452 548 3.12e-24 98.6
MsG0280006735.01.T01 AT4G38470 48.352 91 46 1 31 121 452 541 5.16e-24 97.8
MsG0280006735.01.T01 AT2G17700 42.857 112 59 3 12 122 431 538 7.32e-24 97.4
MsG0280006735.01.T01 AT5G01850 43.529 85 48 0 38 122 198 282 3.90e-23 94.0
MsG0280006735.01.T01 AT3G63260 38.095 126 69 3 31 156 275 391 4.29e-23 94.4
MsG0280006735.01.T01 AT5G01850 43.529 85 48 0 38 122 222 306 5.94e-23 93.6
MsG0280006735.01.T01 AT4G35780 37.398 123 68 2 31 153 454 567 2.91e-22 92.8
MsG0280006735.01.T01 AT5G50000 37.302 126 70 3 31 156 269 385 1.21e-21 90.5
MsG0280006735.01.T01 AT3G01490 35.714 126 72 3 31 156 295 411 1.78e-20 87.4
MsG0280006735.01.T01 AT4G14780 42.105 95 54 1 31 125 248 341 2.94e-20 86.3
MsG0280006735.01.T01 AT3G22750 37.273 110 67 2 31 140 262 369 3.02e-20 86.7
MsG0280006735.01.T01 AT3G24715 50.704 71 35 0 45 115 1025 1095 6.61e-19 83.6
MsG0280006735.01.T01 AT3G24715 50.704 71 35 0 45 115 1025 1095 6.61e-19 83.6
MsG0280006735.01.T01 AT3G24715 50.704 71 35 0 45 115 1025 1095 6.61e-19 83.6
MsG0280006735.01.T01 AT5G57610 39.175 97 58 1 31 126 955 1051 6.63e-19 83.6
MsG0280006735.01.T01 AT5G58520 46.575 73 39 0 47 119 526 598 1.50e-18 82.4
MsG0280006735.01.T01 AT2G35050 42.708 96 54 1 31 125 1147 1242 1.52e-18 82.4
MsG0280006735.01.T01 AT2G35050 42.708 96 54 1 31 125 1147 1242 1.52e-18 82.4
MsG0280006735.01.T01 AT1G04700 43.902 82 46 0 45 126 955 1036 2.12e-18 82.0
MsG0280006735.01.T01 AT1G04700 43.902 82 46 0 45 126 955 1036 2.12e-18 82.0
MsG0280006735.01.T01 AT1G16270 49.296 71 36 0 45 115 1052 1122 2.75e-17 79.0
MsG0280006735.01.T01 AT1G16270 49.296 71 36 0 45 115 1052 1122 2.75e-17 79.0
MsG0280006735.01.T01 AT1G16270 49.296 71 36 0 45 115 1052 1122 2.75e-17 79.0
MsG0280006735.01.T01 AT1G79570 44.444 81 45 0 45 125 1153 1233 4.70e-17 78.2
MsG0280006735.01.T01 AT1G79570 44.444 81 45 0 45 125 1153 1233 4.70e-17 78.2
MsG0280006735.01.T01 AT3G27560 34.444 90 58 1 38 127 206 294 5.78e-17 77.0
MsG0280006735.01.T01 AT5G50180 36.667 90 56 1 38 127 200 288 7.54e-17 76.6
MsG0280006735.01.T01 AT3G46920 42.683 82 47 0 45 126 1061 1142 9.51e-17 77.4
MsG0280006735.01.T01 AT1G14000 41.000 100 56 3 31 129 333 430 9.61e-17 77.0
MsG0280006735.01.T01 AT3G46920 42.683 82 47 0 45 126 995 1076 1.10e-16 77.0
MsG0280006735.01.T01 AT5G40540 34.444 90 58 1 38 127 109 197 1.78e-16 74.7
MsG0280006735.01.T01 AT5G40540 34.444 90 58 1 38 127 130 218 2.21e-16 74.7
MsG0280006735.01.T01 AT5G40540 36.585 82 51 1 38 119 206 286 5.51e-16 74.3
MsG0280006735.01.T01 AT5G07140 36.634 101 61 1 22 119 477 577 7.45e-16 74.7
MsG0280006735.01.T01 AT5G49470 38.710 93 56 1 31 123 369 460 9.94e-16 74.3
MsG0280006735.01.T01 AT1G08720 40.000 90 53 1 31 120 834 922 1.11e-15 74.3
MsG0280006735.01.T01 AT5G49470 38.710 93 56 1 31 123 656 747 1.12e-15 73.9
MsG0280006735.01.T01 AT4G24480 41.772 79 46 0 44 122 853 931 1.18e-15 73.9
MsG0280006735.01.T01 AT5G49470 38.710 93 56 1 31 123 630 721 1.23e-15 73.9
MsG0280006735.01.T01 AT5G49470 38.710 93 56 1 31 123 717 808 1.24e-15 73.9
MsG0280006735.01.T01 AT5G49470 38.710 93 56 1 31 123 529 620 1.24e-15 73.9
MsG0280006735.01.T01 AT4G24480 41.772 79 46 0 44 122 848 926 1.28e-15 73.9
MsG0280006735.01.T01 AT4G24480 41.772 79 46 0 44 122 848 926 1.30e-15 73.9
MsG0280006735.01.T01 AT5G66710 37.500 96 58 2 38 133 251 344 1.45e-15 73.6
MsG0280006735.01.T01 AT1G67890 36.559 93 58 1 31 123 651 742 5.06e-15 72.4
MsG0280006735.01.T01 AT1G18160 36.735 98 61 1 31 128 880 976 1.41e-14 70.9
MsG0280006735.01.T01 AT5G03730 43.820 89 49 1 31 119 718 805 2.00e-14 70.5
MsG0280006735.01.T01 AT5G03730 43.820 89 49 1 31 119 718 805 2.00e-14 70.5
MsG0280006735.01.T01 AT5G11850 34.043 94 61 1 31 124 774 866 6.46e-14 68.9
MsG0280006735.01.T01 AT1G01450 35.644 101 54 3 42 140 222 313 2.44e-13 67.4
MsG0280006735.01.T01 AT4G18950 38.710 93 52 3 31 120 324 414 2.87e-13 67.0
MsG0280006735.01.T01 AT4G18950 38.710 93 52 3 31 120 324 414 3.07e-13 67.0
MsG0280006735.01.T01 AT3G06640 36.559 93 58 1 31 123 611 702 3.27e-13 67.0
MsG0280006735.01.T01 AT3G06640 36.559 93 58 1 31 123 611 702 3.76e-13 67.0
MsG0280006735.01.T01 AT3G06640 36.559 93 58 1 31 123 611 702 3.76e-13 67.0
MsG0280006735.01.T01 AT3G06630 36.559 93 58 1 31 123 599 690 3.89e-13 66.6
MsG0280006735.01.T01 AT4G23050 34.884 86 55 1 31 116 633 717 1.59e-12 65.1
MsG0280006735.01.T01 AT4G23050 34.884 86 55 1 31 116 632 716 1.61e-12 65.1
MsG0280006735.01.T01 AT5G41730 35.897 78 47 2 44 119 430 506 2.30e-12 64.7
MsG0280006735.01.T01 AT5G41730 35.897 78 47 2 44 119 430 506 2.30e-12 64.7
MsG0280006735.01.T01 AT2G31800 31.250 128 83 3 7 130 339 465 2.45e-12 64.3
MsG0280006735.01.T01 AT3G50730 30.000 100 69 1 33 132 211 309 2.60e-12 64.3
MsG0280006735.01.T01 AT1G73660 36.559 93 58 1 31 123 913 1004 3.76e-12 63.9
MsG0280006735.01.T01 AT3G06620 35.484 93 59 1 31 123 659 750 4.22e-12 63.9
MsG0280006735.01.T01 AT1G64300 32.692 104 65 3 39 138 428 530 4.37e-12 63.9
MsG0280006735.01.T01 AT1G64300 32.692 104 65 3 39 138 428 530 4.37e-12 63.9
MsG0280006735.01.T01 AT2G40860 33.645 107 53 4 31 120 109 214 6.40e-12 63.2
MsG0280006735.01.T01 AT2G40860 33.645 107 53 4 31 120 207 312 9.44e-12 62.8
MsG0280006735.01.T01 AT2G40860 33.645 107 53 4 31 120 207 312 9.48e-12 62.8
MsG0280006735.01.T01 AT2G40860 33.645 107 53 4 31 120 207 312 9.65e-12 62.8
MsG0280006735.01.T01 AT4G38470 56.000 50 21 1 31 80 452 500 1.14e-11 62.4
MsG0280006735.01.T01 AT1G67890 40.278 72 42 1 31 102 523 593 2.22e-11 61.6
MsG0280006735.01.T01 AT1G67890 40.278 72 42 1 31 102 651 721 3.18e-11 61.2
MsG0280006735.01.T01 AT1G67890 40.278 72 42 1 31 102 651 721 3.18e-11 61.2
MsG0280006735.01.T01 AT1G08720 41.667 72 41 1 31 102 834 904 3.38e-11 61.2
MsG0280006735.01.T01 AT3G06630 41.667 72 41 1 31 102 599 669 3.58e-11 61.2
MsG0280006735.01.T01 AT3G46930 49.275 69 29 3 31 98 271 334 9.13e-11 59.7
MsG0280006735.01.T01 AT4G08500 36.585 82 49 2 31 110 496 576 9.40e-11 59.7

Find 50 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAAAGATTCATGTGAATTT+TGG 0.165056 2:-5561931 None:intergenic
CGCCTTAAACTTGCAGATTT+CGG 0.183419 2:+5560330 MsG0280006735.01.T01:CDS
CGGATAAGAGGCCTGATTTC+TGG 0.184743 2:+5561749 MsG0280006735.01.T01:CDS
TGCCGAAATCTGCAAGTTTA+AGG 0.242836 2:-5560332 None:intergenic
TTGTATTTCTTCAGAAATTA+AGG 0.251151 2:+5561659 MsG0280006735.01.T01:intron
CTGGAAAATTGTCAAGGTAT+TGG 0.314241 2:+5561768 MsG0280006735.01.T01:CDS
AAGGTTGACGTGTACAGTTT+TGG 0.329020 2:+5560439 MsG0280006735.01.T01:CDS
GGAGGCAGTATGTGACTTAT+TGG 0.364581 2:+5560365 MsG0280006735.01.T01:CDS
GAGGGTTCTGCACCAGAGTC+AGG 0.378959 2:-5561825 None:intergenic
GGTCCCGTGCATCAAAATAA+TGG 0.381630 2:+5561895 MsG0280006735.01.T01:CDS
GTGATCAAACGGAAATCATA+TGG 0.396624 2:+5560412 MsG0280006735.01.T01:CDS
CTCCATTGGATTCAAAAGCT+TGG 0.408751 2:+5561874 MsG0280006735.01.T01:CDS
CAGAAATCAGGCCTCTTATC+CGG 0.412044 2:-5561748 None:intergenic
GCAATGTTGGTCGTTGCAAC+CGG 0.423751 2:+5561729 MsG0280006735.01.T01:CDS
GGGCAGTTTGAAGGAATAAC+TGG 0.431713 2:-5561682 None:intergenic
CAGAGTCAGGGTTCCATCGC+GGG 0.444555 2:-5561812 None:intergenic
AAAAGAAAGGGCTTCTCCAT+TGG 0.445363 2:+5561860 MsG0280006735.01.T01:CDS
TCAGGGTTCCATCGCGGGCA+AGG 0.456880 2:-5561807 None:intergenic
ATCACAGGTGGGCAGTTTGA+AGG 0.459295 2:-5561691 None:intergenic
CACCTACCGTTGTGATCAAA+CGG 0.464742 2:+5560401 MsG0280006735.01.T01:CDS
CGTTGCCCAGATCAAAAGAA+AGG 0.474538 2:+5561847 MsG0280006735.01.T01:CDS
GGTCGTTGCAACCGGATAAG+AGG 0.484450 2:+5561737 MsG0280006735.01.T01:CDS
ATCCTCGTAGGGAATTGTTC+CGG 0.490997 2:-5560485 None:intergenic
ACAGCAAAAGCAGCCTGGAT+TGG 0.492134 2:-5560514 None:intergenic
TCACAACAGCAAAAGCAGCC+TGG 0.502978 2:-5560519 None:intergenic
GAGCTTTGATCGAGCAATGT+TGG 0.503827 2:+5561716 MsG0280006735.01.T01:CDS
TGATCACAACGGTAGGTGCC+AGG 0.508148 2:-5560396 None:intergenic
CCGCGATGGAACCCTGACTC+TGG 0.513228 2:+5561813 MsG0280006735.01.T01:CDS
CGAGGATATGAATCCAATCC+AGG 0.514188 2:+5560501 MsG0280006735.01.T01:CDS
ATCTTATGGGAAATGTTGAC+CGG 0.517700 2:+5560466 MsG0280006735.01.T01:CDS
GAATCTTCCCTTGCCCGCGA+TGG 0.523953 2:+5561799 MsG0280006735.01.T01:CDS
TCGATCAAAGCTCGCATCAC+AGG 0.525605 2:-5561706 None:intergenic
ACGGAAATCATATGGGAGAA+AGG 0.527210 2:+5560420 MsG0280006735.01.T01:CDS
CAGGGTTCCATCGCGGGCAA+GGG 0.533734 2:-5561806 None:intergenic
GACTTATTGGCAGATGATCC+TGG 0.537558 2:+5560378 MsG0280006735.01.T01:CDS
AGATTTCGGCATTGCTTGTG+AGG 0.544952 2:+5560344 MsG0280006735.01.T01:CDS
ATCAAAGCTCGCATCACAGG+TGG 0.553009 2:-5561703 None:intergenic
TGATCAAACGGAAATCATAT+GGG 0.559440 2:+5560413 MsG0280006735.01.T01:CDS
GTTGCCCAGATCAAAAGAAA+GGG 0.560628 2:+5561848 MsG0280006735.01.T01:CDS
GATTGGATTCATATCCTCGT+AGG 0.594679 2:-5560497 None:intergenic
ATTGGATTCATATCCTCGTA+GGG 0.595435 2:-5560496 None:intergenic
TGATTTCTGGAAAATTGTCA+AGG 0.607658 2:+5561762 MsG0280006735.01.T01:CDS
TTTCGGCATTGCTTGTGAGG+AGG 0.618574 2:+5560347 MsG0280006735.01.T01:CDS
AGGGTTCTGCACCAGAGTCA+GGG 0.620195 2:-5561824 None:intergenic
TTCCGTTTGATCACAACGGT+AGG 0.627205 2:-5560403 None:intergenic
GGCGAAAGTCTTCATCAATG+AGG 0.630178 2:-5560311 None:intergenic
TCAAAGCTCGCATCACAGGT+GGG 0.654128 2:-5561702 None:intergenic
CCAGAGTCAGGGTTCCATCG+CGG 0.654386 2:-5561813 None:intergenic
TGATTTCCGTTTGATCACAA+CGG 0.675241 2:-5560407 None:intergenic
GACCGGAACAATTCCCTACG+AGG 0.704958 2:+5560483 MsG0280006735.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGTTGAATATTAGAAAATA+AGG - Chr2:5561113-5561132 None:intergenic 15.0%
!! TTAATAATACTATTTCATGT+TGG - Chr2:5561516-5561535 None:intergenic 15.0%
!!! AAACGACATTTTTAATTTAT+TGG - Chr2:5561015-5561034 None:intergenic 15.0%
!!! TTTTAAAAAATCTAATGTAC+TGG - Chr2:5561228-5561247 None:intergenic 15.0%
!! AACATTATCAATAACAGTAA+CGG - Chr2:5561059-5561078 None:intergenic 20.0%
!! ATTATCGAGAAATATATACA+TGG - Chr2:5561154-5561173 None:intergenic 20.0%
!! TTGTATTTCTTCAGAAATTA+AGG + Chr2:5561659-5561678 MsG0280006735.01.T01:intron 20.0%
!!! AAATTTTGGAGTGAAAAAAA+CGG - Chr2:5560677-5560696 None:intergenic 20.0%
!!! AGTATTATTAATGATTTGCT+TGG + Chr2:5561525-5561544 MsG0280006735.01.T01:intron 20.0%
!!! GTTTTTCGAACAAAAAAATT+GGG + Chr2:5560928-5560947 MsG0280006735.01.T01:intron 20.0%
!!! TGTTTTTCGAACAAAAAAAT+TGG + Chr2:5560927-5560946 MsG0280006735.01.T01:intron 20.0%
! AAGAATAATTCATTAAACGC+CGG - Chr2:5561356-5561375 None:intergenic 25.0%
! ACTACTATAATGAAGTAACT+AGG - Chr2:5561310-5561329 None:intergenic 25.0%
!! ACTGAAACTTTAGATTGTTT+TGG + Chr2:5561476-5561495 MsG0280006735.01.T01:intron 25.0%
!! CTTGGATTTTCATTCTATAT+GGG + Chr2:5560833-5560852 MsG0280006735.01.T01:intron 25.0%
!! TAATTGTATCTTTTCCATGT+TGG - Chr2:5560763-5560782 None:intergenic 25.0%
!! TATTTCGTATCTTTTACGAA+TGG + Chr2:5560737-5560756 MsG0280006735.01.T01:intron 25.0%
!! TCTTGGATTTTCATTCTATA+TGG + Chr2:5560832-5560851 MsG0280006735.01.T01:intron 25.0%
!!! TGAATTTTGGTTTAAGCTTA+GGG - Chr2:5561921-5561940 None:intergenic 25.0%
!!! TTCTTTTCTTTTGAAACTCA+AGG + Chr2:5560713-5560732 MsG0280006735.01.T01:intron 25.0%
!!! TTGTTTTGGATCTTTTTGAA+TGG + Chr2:5561490-5561509 MsG0280006735.01.T01:intron 25.0%
AATTTATTGGAACGCAAGTA+CGG - Chr2:5561002-5561021 None:intergenic 30.0%
AGAATAATTCATTAAACGCC+GGG - Chr2:5561355-5561374 None:intergenic 30.0%
ATATATACATGGCAGGAAAT+AGG - Chr2:5561143-5561162 None:intergenic 30.0%
ATTAGTACTAGGTACATATG+TGG - Chr2:5561266-5561285 None:intergenic 30.0%
CATTATAGTAGTCATTGTAC+AGG + Chr2:5561318-5561337 MsG0280006735.01.T01:intron 30.0%
TAGATGATTTGAACACACAA+AGG - Chr2:5560557-5560576 None:intergenic 30.0%
TATATACATGGCAGGAAATA+GGG - Chr2:5561142-5561161 None:intergenic 30.0%
TATGTACCTAGTACTAATAC+CGG + Chr2:5561268-5561287 MsG0280006735.01.T01:intron 30.0%
TGATCAAACGGAAATCATAT+GGG + Chr2:5560413-5560432 MsG0280006735.01.T01:CDS 30.0%
TGTAAATCTATTCCAAGCAT+TGG - Chr2:5561403-5561422 None:intergenic 30.0%
TTAGTACTAGGTACATATGT+GGG - Chr2:5561265-5561284 None:intergenic 30.0%
TTTCAAAAGAAAAGAAACCG+TGG - Chr2:5560709-5560728 None:intergenic 30.0%
TTTGTGTGTTCAAATCATCT+AGG + Chr2:5560556-5560575 MsG0280006735.01.T01:intron 30.0%
! CTTTGAATTTTTCCAATGCT+TGG + Chr2:5561388-5561407 MsG0280006735.01.T01:intron 30.0%
! GACATGTACGACATTAATTT+TGG - Chr2:5560863-5560882 None:intergenic 30.0%
! GTTCTGATATTTCTGTTATG+CGG + Chr2:5561634-5561653 MsG0280006735.01.T01:intron 30.0%
! TGATTTCTGGAAAATTGTCA+AGG + Chr2:5561762-5561781 MsG0280006735.01.T01:CDS 30.0%
! TTGACAATTTTCCAGAAATC+AGG - Chr2:5561763-5561782 None:intergenic 30.0%
!!! GTGAATTTTGGTTTAAGCTT+AGG - Chr2:5561922-5561941 None:intergenic 30.0%
AAAAAATTGGGTACCCGATT+TGG + Chr2:5560940-5560959 MsG0280006735.01.T01:intron 35.0%
AAAAACGGCTATAATGCACA+AGG - Chr2:5560662-5560681 None:intergenic 35.0%
AATCATTGATGCAGTATGCA+TGG - Chr2:5560811-5560830 None:intergenic 35.0%
AATTAATGTCGTACATGTCC+TGG + Chr2:5560864-5560883 MsG0280006735.01.T01:intron 35.0%
ATCTTATGGGAAATGTTGAC+CGG + Chr2:5560466-5560485 MsG0280006735.01.T01:CDS 35.0%
ATTGGATTCATATCCTCGTA+GGG - Chr2:5560499-5560518 None:intergenic 35.0%
CAGAAATATCAGAACTACAG+AGG - Chr2:5561629-5561648 None:intergenic 35.0%
CTCATAGTGAAAATCGTTGA+TGG - Chr2:5561578-5561597 None:intergenic 35.0%
CTGGAAAATTGTCAAGGTAT+TGG + Chr2:5561768-5561787 MsG0280006735.01.T01:CDS 35.0%
GAAGAACCCATTAACGAAAA+AGG - Chr2:5561450-5561469 None:intergenic 35.0%
GTAACTAGGTTGCATGAATA+TGG - Chr2:5561296-5561315 None:intergenic 35.0%
GTGATCAAACGGAAATCATA+TGG + Chr2:5560412-5560431 MsG0280006735.01.T01:CDS 35.0%
TACTGCATCAATGATTCTCT+TGG + Chr2:5560815-5560834 MsG0280006735.01.T01:intron 35.0%
TAGTACTAGGTACATATGTG+GGG - Chr2:5561264-5561283 None:intergenic 35.0%
TCGAGAAATATATACATGGC+AGG - Chr2:5561150-5561169 None:intergenic 35.0%
TGATTTCCGTTTGATCACAA+CGG - Chr2:5560410-5560429 None:intergenic 35.0%
! CAAAATTTGCTTCTCTTCCA+CGG + Chr2:5560689-5560708 MsG0280006735.01.T01:intron 35.0%
! CATCAACGATTTTCACTATG+AGG + Chr2:5561576-5561595 MsG0280006735.01.T01:intron 35.0%
!! AAAGCCTCCTTTTTCGTTAA+TGG + Chr2:5561440-5561459 MsG0280006735.01.T01:intron 35.0%
!! AAGCCTCCTTTTTCGTTAAT+GGG + Chr2:5561441-5561460 MsG0280006735.01.T01:intron 35.0%
!!! ACAGTTTTGGGCTTATCTTA+TGG + Chr2:5560452-5560471 MsG0280006735.01.T01:CDS 35.0%
!!! CAGTTTTGGGCTTATCTTAT+GGG + Chr2:5560453-5560472 MsG0280006735.01.T01:CDS 35.0%
!!! TGCTTTTGCTGTTGTGAATA+AGG + Chr2:5560525-5560544 MsG0280006735.01.T01:CDS 35.0%
AAAAGAAAGGGCTTCTCCAT+TGG + Chr2:5561860-5561879 MsG0280006735.01.T01:CDS 40.0%
ACGGAAATCATATGGGAGAA+AGG + Chr2:5560420-5560439 MsG0280006735.01.T01:CDS 40.0%
AGGTTGCATGAATATGGATC+CGG - Chr2:5561290-5561309 None:intergenic 40.0%
ATGGATCCGGTATTAGTACT+AGG - Chr2:5561277-5561296 None:intergenic 40.0%
ATTAAACGCCGGGAAAATAC+AGG - Chr2:5561345-5561364 None:intergenic 40.0%
CAGAACTACAGAGGTAAACT+CGG - Chr2:5561620-5561639 None:intergenic 40.0%
CGCCTTAAACTTGCAGATTT+CGG + Chr2:5560330-5560349 MsG0280006735.01.T01:CDS 40.0%
CTCCATTGGATTCAAAAGCT+TGG + Chr2:5561874-5561893 MsG0280006735.01.T01:CDS 40.0%
GAACCCATTAACGAAAAAGG+AGG - Chr2:5561447-5561466 None:intergenic 40.0%
GATTGGATTCATATCCTCGT+AGG - Chr2:5560500-5560519 None:intergenic 40.0%
GTTGCCCAGATCAAAAGAAA+GGG + Chr2:5561848-5561867 MsG0280006735.01.T01:CDS 40.0%
TGCCGAAATCTGCAAGTTTA+AGG - Chr2:5560335-5560354 None:intergenic 40.0%
! AAGGTTGACGTGTACAGTTT+TGG + Chr2:5560439-5560458 MsG0280006735.01.T01:CDS 40.0%
! AGAAGCCCTTTCTTTTGATC+TGG - Chr2:5561856-5561875 None:intergenic 40.0%
! AGGTTGACGTGTACAGTTTT+GGG + Chr2:5560440-5560459 MsG0280006735.01.T01:CDS 40.0%
! CGTGGAAGAGAAGCAAATTT+TGG - Chr2:5560691-5560710 None:intergenic 40.0%
! GAAGCCCTTTCTTTTGATCT+GGG - Chr2:5561855-5561874 None:intergenic 40.0%
! GACCAAGCTTTTGAATCCAA+TGG - Chr2:5561879-5561898 None:intergenic 40.0%
! TCTTTTACGAATGGCCAACA+TGG + Chr2:5560746-5560765 MsG0280006735.01.T01:intron 40.0%
!! AGGCTTTGAAGTGGAAAAAG+AGG - Chr2:5561427-5561446 None:intergenic 40.0%
!!! AGGGCCATTATTTTGATGCA+CGG - Chr2:5561902-5561921 None:intergenic 40.0%
AGATTTCGGCATTGCTTGTG+AGG + Chr2:5560344-5560363 MsG0280006735.01.T01:CDS 45.0%
AGTTAAGTACGCGCTTAACC+AGG - Chr2:5560885-5560904 None:intergenic 45.0%
ATCCTCGTAGGGAATTGTTC+CGG - Chr2:5560488-5560507 None:intergenic 45.0%
CACCTACCGTTGTGATCAAA+CGG + Chr2:5560401-5560420 MsG0280006735.01.T01:CDS 45.0%
CAGAAATCAGGCCTCTTATC+CGG - Chr2:5561751-5561770 None:intergenic 45.0%
CGAGGATATGAATCCAATCC+AGG + Chr2:5560501-5560520 MsG0280006735.01.T01:CDS 45.0%
CGTTGCCCAGATCAAAAGAA+AGG + Chr2:5561847-5561866 MsG0280006735.01.T01:CDS 45.0%
GACTTATTGGCAGATGATCC+TGG + Chr2:5560378-5560397 MsG0280006735.01.T01:CDS 45.0%
GGAGGCAGTATGTGACTTAT+TGG + Chr2:5560365-5560384 MsG0280006735.01.T01:CDS 45.0%
GGCGAAAGTCTTCATCAATG+AGG - Chr2:5560314-5560333 None:intergenic 45.0%
GGGCAGTTTGAAGGAATAAC+TGG - Chr2:5561685-5561704 None:intergenic 45.0%
GGTCCCGTGCATCAAAATAA+TGG + Chr2:5561895-5561914 MsG0280006735.01.T01:CDS 45.0%
TTCCGTTTGATCACAACGGT+AGG - Chr2:5560406-5560425 None:intergenic 45.0%
! GAGCTTTGATCGAGCAATGT+TGG + Chr2:5561716-5561735 MsG0280006735.01.T01:CDS 45.0%
! GTACAGGACCTGTATTTTCC+CGG + Chr2:5561334-5561353 MsG0280006735.01.T01:intron 45.0%
! TCTTTTGATCTGGGCAACGA+GGG - Chr2:5561846-5561865 None:intergenic 45.0%
! TTCTTTTGATCTGGGCAACG+AGG - Chr2:5561847-5561866 None:intergenic 45.0%
!! CGAAAAAGGAGGCTTTGAAG+TGG - Chr2:5561436-5561455 None:intergenic 45.0%
!!! GGGCCATTATTTTGATGCAC+GGG - Chr2:5561901-5561920 None:intergenic 45.0%
ACAGCAAAAGCAGCCTGGAT+TGG - Chr2:5560517-5560536 None:intergenic 50.0%
ATCAAAGCTCGCATCACAGG+TGG - Chr2:5561706-5561725 None:intergenic 50.0%
ATCACAGGTGGGCAGTTTGA+AGG - Chr2:5561694-5561713 None:intergenic 50.0%
CGGATAAGAGGCCTGATTTC+TGG + Chr2:5561749-5561768 MsG0280006735.01.T01:CDS 50.0%
GCAATGTTGGTCGTTGCAAC+CGG + Chr2:5561729-5561748 MsG0280006735.01.T01:CDS 50.0%
TACCCCACGTGTGTCCAAAT+CGG - Chr2:5560957-5560976 None:intergenic 50.0%
TCAAAGCTCGCATCACAGGT+GGG - Chr2:5561705-5561724 None:intergenic 50.0%
TCACAACAGCAAAAGCAGCC+TGG - Chr2:5560522-5560541 None:intergenic 50.0%
TCGATCAAAGCTCGCATCAC+AGG - Chr2:5561709-5561728 None:intergenic 50.0%
TTTCGGCATTGCTTGTGAGG+AGG + Chr2:5560347-5560366 MsG0280006735.01.T01:CDS 50.0%
!! GAACGCAAGTACGGACTAGT+AGG - Chr2:5560993-5561012 None:intergenic 50.0%
!! TACCCGATTTGGACACACGT+GGG + Chr2:5560951-5560970 MsG0280006735.01.T01:intron 50.0%
ACCCCACGTGTGTCCAAATC+GGG - Chr2:5560956-5560975 None:intergenic 55.0%
AGGGTTCTGCACCAGAGTCA+GGG - Chr2:5561827-5561846 None:intergenic 55.0%
GACCGGAACAATTCCCTACG+AGG + Chr2:5560483-5560502 MsG0280006735.01.T01:CDS 55.0%
GGTCGTTGCAACCGGATAAG+AGG + Chr2:5561737-5561756 MsG0280006735.01.T01:CDS 55.0%
TGATCACAACGGTAGGTGCC+AGG - Chr2:5560399-5560418 None:intergenic 55.0%
!! ACCCGATTTGGACACACGTG+GGG + Chr2:5560952-5560971 MsG0280006735.01.T01:intron 55.0%
!! GTACCCGATTTGGACACACG+TGG + Chr2:5560950-5560969 MsG0280006735.01.T01:intron 55.0%
CAGAGTCAGGGTTCCATCGC+GGG - Chr2:5561815-5561834 None:intergenic 60.0%
CCAGAGTCAGGGTTCCATCG+CGG - Chr2:5561816-5561835 None:intergenic 60.0%
GAATCTTCCCTTGCCCGCGA+TGG + Chr2:5561799-5561818 MsG0280006735.01.T01:CDS 60.0%
GAGGGTTCTGCACCAGAGTC+AGG - Chr2:5561828-5561847 None:intergenic 60.0%
CAGGGTTCCATCGCGGGCAA+GGG - Chr2:5561809-5561828 None:intergenic 65.0%
CCGCGATGGAACCCTGACTC+TGG + Chr2:5561813-5561832 MsG0280006735.01.T01:CDS 65.0%
TCAGGGTTCCATCGCGGGCA+AGG - Chr2:5561810-5561829 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 5560307 5561945 5560307 ID=MsG0280006735.01;Name=MsG0280006735.01
Chr2 mRNA 5560307 5561945 5560307 ID=MsG0280006735.01.T01;Parent=MsG0280006735.01;Name=MsG0280006735.01.T01;_AED=0.49;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|170
Chr2 exon 5560307 5560546 5560307 ID=MsG0280006735.01.T01:exon:2250;Parent=MsG0280006735.01.T01
Chr2 exon 5561673 5561945 5561673 ID=MsG0280006735.01.T01:exon:2251;Parent=MsG0280006735.01.T01
Chr2 CDS 5560307 5560546 5560307 ID=MsG0280006735.01.T01:cds;Parent=MsG0280006735.01.T01
Chr2 CDS 5561673 5561945 5561673 ID=MsG0280006735.01.T01:cds;Parent=MsG0280006735.01.T01
Gene Sequence

>MsG0280006735.01.T01

ATGTCCTCATTGATGAAGACTTTCGCCTTAAACTTGCAGATTTCGGCATTGCTTGTGAGGAGGCAGTATGTGACTTATTGGCAGATGATCCTGGCACCTACCGTTGTGATCAAACGGAAATCATATGGGAGAAAGGTTGACGTGTACAGTTTTGGGCTTATCTTATGGGAAATGTTGACCGGAACAATTCCCTACGAGGATATGAATCCAATCCAGGCTGCTTTTGCTGTTGTGAATAAGAAATTAAGGCCAGTTATTCCTTCAAACTGCCCACCTGTGATGCGAGCTTTGATCGAGCAATGTTGGTCGTTGCAACCGGATAAGAGGCCTGATTTCTGGAAAATTGTCAAGGTATTGGAGCAATTTGAATCTTCCCTTGCCCGCGATGGAACCCTGACTCTGGTGCAGAACCCTCGTTGCCCAGATCAAAAGAAAGGGCTTCTCCATTGGATTCAAAAGCTTGGTCCCGTGCATCAAAATAATGGCCCTAAGCTTAAACCAAAATTCACATGA
Protein sequence

>MsG0280006735.01.T01

MSSLMKTFALNLQISALLVRRQYVTYWQMILAPTVVIKRKSYGRKVDVYSFGLILWEMLTGTIPYEDMNPIQAAFAVVNKKLRPVIPSNCPPVMRALIEQCWSLQPDKRPDFWKIVKVLEQFESSLARDGTLTLVQNPRCPDQKKGLLHWIQKLGPVHQNNGPKLKPKFT*