AlfalfaGEDB | MsG0280006971.01

M. sativa cultivar ZhongmuNo.1 / MsG0280006971.01

KEGG: -
GO: GO:0003677 GO:0006355
PFAM: PF02701

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006971.01.T02	XP_039685435.1	69.725	109	32	1	1	108	10	118	1.44E-33	126

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006971.01.T02	O82155	47.664	107	52	1	2	104	24	130	7.53E-24	93.2

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006971.01.T02	G8A2U0	69.725	109	32	1	1	108	10	118	6.89e-34	126

TFs/TRs:

Gene ID	Type	Classification
MsG0280006971.01.T01	TF	C2C2-Dof
MsG0280006971.01.T02	TF	C2C2-Dof

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006971.01.T01	MTR_1g115590	69.697	33	10	0	1	33	10	42	5.55e-12	57.4
MsG0280006971.01.T01	MTR_7g082600	66.667	33	11	0	1	33	1	33	5.09e-11	55.1
MsG0280006971.01.T02	MTR_8g015840	69.725	109	32	1	1	108	10	118	1.75e-37	126
MsG0280006971.01.T02	MTR_8g079060	50.633	79	39	0	6	84	24	102	2.30e-20	82.4
MsG0280006971.01.T02	MTR_2g096740	47.423	97	45	2	6	97	57	152	7.76e-19	79.3
MsG0280006971.01.T02	MTR_2g096740	47.423	97	45	2	6	97	72	167	1.01e-18	79.3
MsG0280006971.01.T02	MTR_1g115590	56.881	109	17	2	1	109	10	88	4.96e-17	71.6
MsG0280006971.01.T02	MTR_4g063780	41.379	87	50	1	6	92	70	155	2.68e-16	72.8
MsG0280006971.01.T02	MTR_7g024670	52.308	65	31	0	12	76	78	142	3.88e-16	72.8
MsG0280006971.01.T02	MTR_7g082600	56.881	109	17	3	1	109	1	79	3.96e-16	69.3
MsG0280006971.01.T02	MTR_8g479350	56.364	55	24	0	8	62	65	119	6.22e-16	72.0
MsG0280006971.01.T02	MTR_7g059400	44.660	103	54	3	4	104	68	169	6.44e-16	72.0
MsG0280006971.01.T02	MTR_5g041400	46.237	93	42	2	11	95	119	211	1.85e-15	70.9
MsG0280006971.01.T02	MTR_4g088580	46.250	80	41	1	12	89	56	135	2.43e-15	70.5
MsG0280006971.01.T02	MTR_4g022370	45.263	95	46	3	4	93	74	167	5.22e-15	69.3
MsG0280006971.01.T02	MTR_4g461080	54.098	61	28	0	2	62	20	80	7.21e-15	68.6
MsG0280006971.01.T02	MTR_6g012450	44.928	69	38	0	11	79	136	204	7.87e-15	69.3
MsG0280006971.01.T02	MTR_5g041380	45.161	93	43	2	11	95	127	219	8.32e-15	68.9
MsG0280006971.01.T02	MTR_8g027295	39.000	100	43	3	8	106	21	103	1.70e-14	67.4
MsG0280006971.01.T02	MTR_4g109980	56.604	53	23	0	9	61	38	90	2.44e-14	67.4
MsG0280006971.01.T02	MTR_2g030030	54.128	109	20	2	1	109	10	88	7.78e-14	63.2
MsG0280006971.01.T02	MTR_2g059540	72.973	37	10	0	11	47	31	67	8.44e-14	64.7
MsG0280006971.01.T02	MTR_5g031440	50.000	56	28	0	6	61	38	93	9.40e-14	65.9
MsG0280006971.01.T02	MTR_5g041530	47.619	63	33	0	13	75	142	204	1.04e-13	65.9
MsG0280006971.01.T02	MTR_2g014170	89.655	29	3	0	11	39	45	73	1.12e-13	65.5
MsG0280006971.01.T02	MTR_8g068210	47.826	69	32	1	11	79	32	96	1.54e-13	65.1
MsG0280006971.01.T02	MTR_3g091820	50.000	64	30	2	6	68	37	99	2.42e-13	64.7
MsG0280006971.01.T02	MTR_3g435480	44.928	69	38	0	11	79	136	204	3.30e-13	64.3
MsG0280006971.01.T02	MTR_1g077600	46.875	64	34	0	2	65	34	97	5.69e-13	63.2
MsG0280006971.01.T02	MTR_1g077600	46.875	64	34	0	2	65	34	97	6.08e-13	63.2
MsG0280006971.01.T02	MTR_4g082060	50.000	52	26	0	11	62	122	173	6.97e-13	63.5
MsG0280006971.01.T02	MTR_7g010950	47.059	68	35	1	13	80	137	203	9.39e-13	63.2
MsG0280006971.01.T02	MTR_5g041420	48.333	60	30	1	3	62	131	189	1.25e-12	62.8
MsG0280006971.01.T02	MTR_7g086780	38.356	73	45	0	11	83	99	171	1.79e-12	62.4
MsG0280006971.01.T02	MTR_4g089095	77.419	31	7	0	9	39	48	78	1.88e-12	62.0
MsG0280006971.01.T02	MTR_4g089095	77.419	31	7	0	9	39	14	44	2.05e-12	61.6
MsG0280006971.01.T02	MTR_3g077750	50.000	56	28	0	6	61	56	111	2.11e-12	62.0
MsG0280006971.01.T02	MTR_2g093220	52.632	57	26	1	6	62	36	91	2.43e-12	61.6
MsG0280006971.01.T02	MTR_3g090430	56.000	50	22	0	13	62	53	102	2.75e-12	61.6
MsG0280006971.01.T02	MTR_2g016030	47.170	53	28	0	11	63	47	99	3.29e-12	59.7
MsG0280006971.01.T02	MTR_6g027460	36.486	74	47	0	11	84	96	169	3.98e-12	61.2
MsG0280006971.01.T02	MTR_2g013370	74.194	31	8	0	9	39	44	74	4.23e-12	60.8
MsG0280006971.01.T02	MTR_2g014060	52.941	51	24	0	12	62	26	76	4.38e-12	61.2
MsG0280006971.01.T02	MTR_8g044220	48.077	52	27	0	11	62	118	169	6.28e-12	60.8
MsG0280006971.01.T02	MTR_1g056810	50.847	59	27	1	13	71	14	70	9.35e-12	60.1
MsG0280006971.01.T02	MTR_6g027450	39.744	78	46	1	11	88	89	165	1.14e-11	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006971.01.T02	AT1G51700	47.664	107	52	1	2	104	24	130	7.68e-25	93.2
MsG0280006971.01.T02	AT3G50410	54.795	73	27	1	1	67	14	86	4.30e-20	82.0
MsG0280006971.01.T02	AT5G66940	50.704	71	35	0	7	77	28	98	2.13e-18	77.0
MsG0280006971.01.T02	AT1G07640	49.231	65	33	0	6	70	16	80	2.06e-16	72.8
MsG0280006971.01.T02	AT1G07640	48.485	66	34	0	6	71	72	137	3.44e-16	72.4
MsG0280006971.01.T02	AT1G07640	48.485	66	34	0	6	71	80	145	3.60e-16	72.4
MsG0280006971.01.T02	AT5G60850	49.367	79	36	1	9	87	49	123	3.83e-16	72.4
MsG0280006971.01.T02	AT3G21270	59.649	57	22	1	11	66	29	85	4.05e-16	70.9
MsG0280006971.01.T02	AT5G60200	43.023	86	47	1	6	89	50	135	1.76e-15	70.1
MsG0280006971.01.T02	AT5G65590	47.945	73	31	1	7	79	37	102	2.94e-15	70.1
MsG0280006971.01.T02	AT3G45610	49.315	73	34	2	6	76	35	106	3.20e-15	68.9
MsG0280006971.01.T02	AT2G28810	49.123	57	29	0	6	62	89	145	7.59e-15	68.9
MsG0280006971.01.T02	AT2G28810	49.123	57	29	0	6	62	72	128	7.64e-15	68.9
MsG0280006971.01.T02	AT3G52440	44.304	79	40	1	13	87	27	105	6.83e-14	65.5
MsG0280006971.01.T02	AT3G52440	44.304	79	40	1	13	87	46	124	8.64e-14	65.5
MsG0280006971.01.T02	AT2G37590	49.091	55	28	0	8	62	86	140	1.03e-13	65.9
MsG0280006971.01.T02	AT3G61850	44.444	72	33	1	8	79	59	123	1.66e-13	64.7
MsG0280006971.01.T02	AT3G61850	44.444	72	33	1	8	79	59	123	1.66e-13	64.7
MsG0280006971.01.T02	AT1G21340	47.826	69	33	1	7	72	31	99	1.78e-13	64.7
MsG0280006971.01.T02	AT3G61850	44.444	72	33	1	8	79	59	123	1.91e-13	64.7
MsG0280006971.01.T02	AT3G61850	47.297	74	28	2	8	79	71	135	1.96e-13	64.7
MsG0280006971.01.T02	AT4G38000	43.038	79	45	0	8	86	38	116	1.97e-13	64.3
MsG0280006971.01.T02	AT3G61850	44.444	72	33	1	8	79	71	135	2.17e-13	64.7
MsG0280006971.01.T02	AT5G39660	53.846	52	24	0	11	62	138	189	2.92e-13	64.7
MsG0280006971.01.T02	AT5G39660	53.846	52	24	0	11	62	138	189	2.92e-13	64.7
MsG0280006971.01.T02	AT5G39660	53.846	52	24	0	11	62	138	189	2.92e-13	64.7
MsG0280006971.01.T02	AT1G28310	89.655	29	3	0	11	39	27	55	3.02e-13	64.3
MsG0280006971.01.T02	AT2G28510	53.226	62	28	1	9	70	46	106	3.21e-13	63.9
MsG0280006971.01.T02	AT3G55370	44.118	68	37	1	8	75	73	139	3.38e-13	64.3
MsG0280006971.01.T02	AT1G28310	89.655	29	3	0	11	39	51	79	3.43e-13	64.3
MsG0280006971.01.T02	AT3G55370	44.118	68	37	1	8	75	73	139	3.62e-13	64.3
MsG0280006971.01.T02	AT3G47500	47.826	69	35	1	11	79	110	177	3.70e-13	64.3
MsG0280006971.01.T02	AT3G55370	44.118	68	37	1	8	75	118	184	3.88e-13	64.3
MsG0280006971.01.T02	AT1G26790	51.923	52	25	0	11	62	111	162	7.47e-13	63.5
MsG0280006971.01.T02	AT1G69570	50.000	52	26	0	11	62	132	183	1.03e-12	63.2
MsG0280006971.01.T02	AT2G46590	75.000	32	8	0	8	39	77	108	1.17e-12	62.8
MsG0280006971.01.T02	AT2G46590	75.000	32	8	0	8	39	65	96	1.22e-12	62.8
MsG0280006971.01.T02	AT4G24060	48.276	58	29	1	6	62	47	104	1.28e-12	62.8
MsG0280006971.01.T02	AT1G29160	48.077	52	27	0	11	62	62	113	2.57e-12	60.1
MsG0280006971.01.T02	AT5G02460	48.333	60	30	1	4	62	87	146	3.48e-12	61.6
MsG0280006971.01.T02	AT5G62430	51.923	52	25	0	11	62	54	105	5.41e-12	60.8
MsG0280006971.01.T02	AT1G47655	48.077	52	27	0	9	60	27	78	5.96e-12	59.7
MsG0280006971.01.T02	AT5G62940	50.000	56	28	0	6	61	68	123	6.08e-12	60.8
MsG0280006971.01.T02	AT2G34140	46.154	52	28	0	11	62	58	109	7.17e-12	58.9
MsG0280006971.01.T02	AT1G64620	71.429	35	9	1	6	39	43	77	1.75e-11	59.7
MsG0280006971.01.T02	AT4G00940	48.276	58	30	0	8	65	65	122	2.76e-11	58.9
MsG0280006971.01.T02	AT4G00940	48.276	58	30	0	8	65	65	122	2.76e-11	58.9
MsG0280006971.01.T02	AT4G00940	48.276	58	30	0	8	65	65	122	2.76e-11	58.9

Find 20 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCTGGGTCGGGTCTGTTTC+TGG	0.149721	2:+8800405	None:intergenic
GGACAAAAGGTGGTGCTTTA+AGG	0.182533	2:-8800559	MsG0280006971.01.T01:CDS
TGTTCTTGTTCTGGAAAATT+TGG	0.207790	2:+8800687	None:intergenic
TGACCCGATGTGAGAAATTT+TGG	0.261678	2:-8800376	MsG0280006971.01.T01:CDS
TTCTGGAAAATTTGGTTTCA+TGG	0.271012	2:+8800695	None:intergenic
CATTTGAGTTGTTCTTGTTC+TGG	0.291270	2:+8800678	None:intergenic
ACATCGGGTCAACATGAATC+TGG	0.347289	2:+8800388	None:intergenic
TGCAAAAGATATTGGACAAA+AGG	0.422430	2:-8800572	MsG0280006971.01.T01:CDS
TCTGGAAAATTTGGTTTCAT+GGG	0.442267	2:+8800696	None:intergenic
TCACCAAAATTTCTCACATC+GGG	0.472907	2:+8800373	None:intergenic
GAAGAAGTGGATGATGTTGA+TGG	0.514146	2:+8800453	None:intergenic
AAGGAACACACCAATGGTGT+TGG	0.522382	2:-8800540	MsG0280006971.01.T01:CDS
GGTCAACATGAATCTGGGTC+GGG	0.540184	2:+8800394	None:intergenic
CATCGGGTCAACATGAATCT+GGG	0.544628	2:+8800389	None:intergenic
GGGTCAACATGAATCTGGGT+CGG	0.568508	2:+8800393	None:intergenic
TTCACCAAAATTTCTCACAT+CGG	0.587351	2:+8800372	None:intergenic
GTTGATGGAGAAGATGAAGA+TGG	0.610885	2:+8800468	None:intergenic
TGCTTTAAGGAACACACCAA+TGG	0.617059	2:-8800546	MsG0280006971.01.T01:CDS
AAAAGATATTGGACAAAAGG+TGG	0.617217	2:-8800569	MsG0280006971.01.T01:CDS
GTGTTGCTTGATTCACAACG+TGG	0.731087	2:+8800654	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CAAAAAATTGCAAAAGATAT+TGG	-	Chr2:8800486-8800505	MsG0280006971.01.T01:CDS	20.0%
!!!	AATTTTTTGCAAAAATGACG+TGG	+	Chr2:8800475-8800494	None:intergenic	25.0%
	AAAAGATATTGGACAAAAGG+TGG	-	Chr2:8800497-8800516	MsG0280006971.01.T01:CDS	30.0%
	TGCAAAAGATATTGGACAAA+AGG	-	Chr2:8800494-8800513	MsG0280006971.01.T01:CDS	30.0%
!!	TGTTCTTGTTCTGGAAAATT+TGG	+	Chr2:8800382-8800401	None:intergenic	30.0%
	TCACCAAAATTTCTCACATC+GGG	+	Chr2:8800696-8800715	None:intergenic	35.0%
!	AACTTTTGTTGCTGAAGAAG+TGG	+	Chr2:8800629-8800648	None:intergenic	35.0%
!	TTTTCTTGTTCCAACACCAT+TGG	+	Chr2:8800539-8800558	None:intergenic	35.0%
!!	CATTTGAGTTGTTCTTGTTC+TGG	+	Chr2:8800391-8800410	None:intergenic	35.0%
	GAAGAAGTGGATGATGTTGA+TGG	+	Chr2:8800616-8800635	None:intergenic	40.0%
!	GTTGATGGAGAAGATGAAGA+TGG	+	Chr2:8800601-8800620	None:intergenic	40.0%
!	TGACCCGATGTGAGAAATTT+TGG	-	Chr2:8800690-8800709	MsG0280006971.01.T01:intron	40.0%
!!	TGCTTTAAGGAACACACCAA+TGG	-	Chr2:8800520-8800539	MsG0280006971.01.T01:CDS	40.0%
	AAGGAACACACCAATGGTGT+TGG	-	Chr2:8800526-8800545	MsG0280006971.01.T01:CDS	45.0%
	ACATCGGGTCAACATGAATC+TGG	+	Chr2:8800681-8800700	None:intergenic	45.0%
	CATCGGGTCAACATGAATCT+GGG	+	Chr2:8800680-8800699	None:intergenic	45.0%
	GGACAAAAGGTGGTGCTTTA+AGG	-	Chr2:8800507-8800526	MsG0280006971.01.T01:CDS	45.0%
!!	GTGTTGCTTGATTCACAACG+TGG	+	Chr2:8800415-8800434	None:intergenic	45.0%
	GGGTCAACATGAATCTGGGT+CGG	+	Chr2:8800676-8800695	None:intergenic	50.0%
	GGTCAACATGAATCTGGGTC+GGG	+	Chr2:8800675-8800694	None:intergenic	50.0%
	ATCTGGGTCGGGTCTGTTTC+TGG	+	Chr2:8800664-8800683	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	8800373	8800715	8800373	ID=MsG0280006971.01;Name=MsG0280006971.01
Chr2	mRNA	8800373	8800715	8800373	ID=MsG0280006971.01.T01;Parent=MsG0280006971.01;Name=MsG0280006971.01.T01;_AED=0.19;_eAED=0.26;_QI=0\|0\|0\|1\|0\|0\|2\|0\|83
Chr2	exon	8800373	8800599	8800373	ID=MsG0280006971.01.T01:exon:2548;Parent=MsG0280006971.01.T01
Chr2	exon	8800691	8800715	8800691	ID=MsG0280006971.01.T01:exon:2547;Parent=MsG0280006971.01.T01
Chr2	CDS	8800691	8800715	8800691	ID=MsG0280006971.01.T01:cds;Parent=MsG0280006971.01.T01
Chr2	CDS	8800373	8800599	8800373	ID=MsG0280006971.01.T01:cds;Parent=MsG0280006971.01.T01
Chr2	mRNA	8800386	8800715	8800386	ID=MsG0280006971.01.T02;Parent=MsG0280006971.01;Name=MsG0280006971.01.T02;_AED=0.10;_eAED=0.10;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|109
Chr2	exon	8800386	8800715	8800386	ID=MsG0280006971.01.T02:exon:2549;Parent=MsG0280006971.01.T02
Chr2	CDS	8800386	8800715	8800386	ID=MsG0280006971.01.T02:cds;Parent=MsG0280006971.01.T02

Gene Sequence

>MsG0280006971.01.T01

ATGAAACCAAATTTTCCAGAACAAGAAAATTGCAAAAGATATTGGACAAAAGGTGGTGCTTTAAGGAACACACCAATGGTGTTGGAACAAGAAAAGTTACAAAACGTTCATCAAATTCTAAACGTTCAACAACACCATCTTCATCTTCTCCATCAACATCATCCACTTCTTCAGCAACAAAAGTTTCAGTTTCAGTTCCAGAAACAGACCCGACCCAGATTCATGTTGACCCGATGTGAGAAATTTTGGTGA

>MsG0280006971.01.T02

ATGAAACCAAATTTTCCAGAACAAGAACAACTCAAATGTCCACGTTGTGAATCAAGCAACACAAAATTATGCTACTACAACAACTACAATCTCTCACAACCACGTCATTTTTGCAAAAAATTGCAAAAGATATTGGACAAAAGGTGGTGCTTTAAGGAACACACCAATGGTGTTGGAACAAGAAAAGTTACAAAACGTTCATCAAATTCTAAACGTTCAACAACACCATCTTCATCTTCTCCATCAACATCATCCACTTCTTCAGCAACAAAAGTTTCAGTTTCAGTTCCAGAAACAGACCCGACCCAGATTCATGTTGACCCGATGTGA

Protein sequence

>MsG0280006971.01.T01

MKPNFPEQENCKRYWTKGGALRNTPMVLEQEKLQNVHQILNVQQHHLHLLHQHHPLLQQQKFQFQFQKQTRPRFMLTRCEKFW*

>MsG0280006971.01.T02

MKPNFPEQEQLKCPRCESSNTKLCYYNNYNLSQPRHFCKKLQKILDKRWCFKEHTNGVGTRKVTKRSSNSKRSTTPSSSSPSTSSTSSATKVSVSVPETDPTQIHVDPM*