AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007063.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007063.01.T01 MTR_2g019810 86.154 195 18 2 1 194 1 187 8.01e-107 311
MsG0280007063.01.T01 MTR_2g019840 73.134 201 49 2 1 196 1 201 3.59e-97 287
MsG0280007063.01.T01 MTR_2g436460 48.810 168 86 0 30 197 39 206 1.94e-47 159
MsG0280007063.01.T01 MTR_2g436440 45.745 188 92 3 12 194 22 204 1.15e-43 150
MsG0280007063.01.T01 MTR_4g119550 47.853 163 84 1 33 194 46 208 3.12e-43 149
MsG0280007063.01.T01 MTR_2g437040 42.391 184 95 3 13 194 24 198 1.22e-39 139
MsG0280007063.01.T01 MTR_2g437020 43.781 201 96 4 1 193 5 196 1.23e-39 139
MsG0280007063.01.T01 MTR_2g437060 42.857 189 97 3 7 193 33 212 1.73e-39 139
MsG0280007063.01.T01 MTR_2g437080 43.407 182 92 3 14 193 25 197 2.51e-39 138
MsG0280007063.01.T01 MTR_2g437030 43.243 185 103 1 12 194 100 284 1.74e-38 138
MsG0280007063.01.T01 MTR_2g436400 43.293 164 91 1 32 193 41 204 3.54e-38 135
MsG0280007063.01.T01 MTR_2g436370 45.098 153 82 1 35 185 43 195 4.20e-38 130
MsG0280007063.01.T01 MTR_2g437260 40.678 177 104 1 18 193 15 191 2.24e-37 134
MsG0280007063.01.T01 MTR_4g120380 45.122 164 88 2 31 193 37 199 6.08e-37 132
MsG0280007063.01.T01 MTR_4g120380 45.122 164 88 2 31 193 37 199 1.15e-36 132
MsG0280007063.01.T01 MTR_2g437130 41.489 188 107 1 7 194 15 199 1.14e-35 129
MsG0280007063.01.T01 MTR_4g007490 40.331 181 103 2 22 198 21 200 2.81e-30 114
MsG0280007063.01.T01 MTR_3g450500 38.983 177 101 2 29 198 26 202 4.78e-29 111
MsG0280007063.01.T01 MTR_2g437160 39.053 169 99 3 30 194 55 223 3.26e-28 109
MsG0280007063.01.T01 MTR_2g437960 37.126 167 100 3 38 199 53 219 3.49e-28 109
MsG0280007063.01.T01 MTR_2g437960 37.126 167 100 3 38 199 53 219 3.66e-28 109
MsG0280007063.01.T01 MTR_4g119580 37.791 172 107 0 22 193 28 199 7.24e-28 108
MsG0280007063.01.T01 MTR_2g076320 39.080 174 97 4 28 194 40 211 2.42e-27 107
MsG0280007063.01.T01 MTR_2g437120 37.500 168 100 3 32 194 45 212 3.01e-27 107
MsG0280007063.01.T01 MTR_2g437200 34.101 217 103 3 17 194 11 226 3.88e-27 106
MsG0280007063.01.T01 MTR_2g437240 44.882 127 65 1 34 160 28 149 2.92e-25 101
MsG0280007063.01.T01 MTR_2g437180 36.257 171 104 3 34 199 45 215 7.02e-25 100
MsG0280007063.01.T01 MTR_2g437990 41.727 139 79 2 28 164 40 178 1.18e-24 100
MsG0280007063.01.T01 MTR_2g438020 50.000 122 54 2 40 160 73 188 1.95e-24 100
MsG0280007063.01.T01 MTR_2g438010 36.782 174 101 5 28 194 60 231 3.57e-24 98.6
MsG0280007063.01.T01 MTR_2g437170 38.554 166 94 4 13 170 27 192 4.04e-24 98.6
MsG0280007063.01.T01 MTR_2g437150 39.456 147 84 3 29 170 46 192 4.16e-24 98.6
MsG0280007063.01.T01 MTR_2g437100 39.130 138 79 3 38 170 60 197 4.27e-22 92.8
MsG0280007063.01.T01 MTR_2g437940 38.028 142 75 5 34 164 45 184 5.21e-16 75.5
MsG0280007063.01.T01 MTR_8g080990 30.814 172 115 3 31 199 35 205 3.25e-15 73.2
MsG0280007063.01.T01 MTR_8g081000 30.357 168 113 3 33 197 50 216 2.01e-14 71.2
MsG0280007063.01.T01 MTR_8g081000 30.357 168 113 3 33 197 50 216 2.09e-14 71.2
MsG0280007063.01.T01 MTR_8g081000 30.357 168 113 3 33 197 50 216 2.23e-14 70.9
MsG0280007063.01.T01 MTR_4g049400 36.667 120 72 2 20 135 30 149 1.17e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007063.01.T01 AT5G07900 35.673 171 107 2 31 198 61 231 1.66e-30 115
MsG0280007063.01.T01 AT1G21150 39.264 163 96 1 38 197 55 217 5.35e-29 111
MsG0280007063.01.T01 AT1G21150 39.264 163 96 1 38 197 119 281 1.58e-28 111
MsG0280007063.01.T01 AT1G21150 37.654 162 98 1 39 197 20 181 4.19e-27 105
MsG0280007063.01.T01 AT1G62085 35.172 145 94 0 16 160 32 176 2.48e-24 99.8
MsG0280007063.01.T01 AT1G62085 35.172 145 94 0 16 160 32 176 2.48e-24 99.8
MsG0280007063.01.T01 AT1G62120 35.115 131 85 0 30 160 47 177 8.51e-24 97.8
MsG0280007063.01.T01 AT1G61960 35.032 157 99 2 36 190 49 204 2.48e-22 94.0
MsG0280007063.01.T01 AT1G61980 33.544 158 101 3 34 189 47 202 8.08e-22 92.4
MsG0280007063.01.T01 AT1G61980 33.544 158 101 3 34 189 47 202 8.08e-22 92.4
MsG0280007063.01.T01 AT1G61980 33.544 158 101 3 34 189 47 202 8.08e-22 92.4
MsG0280007063.01.T01 AT3G46950 32.335 167 106 3 30 195 43 203 1.59e-21 91.7
MsG0280007063.01.T01 AT1G61970 33.333 156 100 2 36 189 49 202 1.21e-20 89.0
MsG0280007063.01.T01 AT1G61970 33.333 156 100 2 36 189 49 202 1.21e-20 89.0
MsG0280007063.01.T01 AT1G61970 33.333 156 100 2 36 189 49 202 1.21e-20 89.0
MsG0280007063.01.T01 AT1G61970 33.333 156 100 2 36 189 49 202 1.21e-20 89.0
MsG0280007063.01.T01 AT1G61970 33.333 156 100 2 36 189 49 202 1.21e-20 89.0
MsG0280007063.01.T01 AT1G61970 33.333 156 100 2 36 189 49 202 1.21e-20 89.0
MsG0280007063.01.T01 AT1G61970 33.333 156 100 2 36 189 49 202 1.21e-20 89.0
MsG0280007063.01.T01 AT5G23930 35.385 130 83 1 30 159 46 174 2.92e-20 88.2
MsG0280007063.01.T01 AT1G62110 35.115 131 85 0 30 160 43 173 3.57e-20 87.8
MsG0280007063.01.T01 AT1G62150 31.250 128 88 0 33 160 52 179 7.59e-19 84.0
MsG0280007063.01.T01 AT1G61990 34.400 125 79 1 36 160 49 170 7.70e-19 84.0
MsG0280007063.01.T01 AT1G61990 34.400 125 79 1 36 160 49 170 7.70e-19 84.0
MsG0280007063.01.T01 AT1G62010 29.496 139 96 1 59 197 58 194 2.49e-14 70.9
MsG0280007063.01.T01 AT1G56380 31.452 124 85 0 33 156 10 133 1.28e-13 68.9
MsG0280007063.01.T01 AT1G56380 31.452 124 85 0 33 156 21 144 1.39e-13 68.9

Find 26 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAGAAGGAAATTGAATTT+AGG 0.170730 2:-10097052 None:intergenic
TGACTGTAAAAGAGTGATTT+TGG 0.173839 2:-10096832 None:intergenic
AGACAACATAAGAGGATTTC+TGG 0.180313 2:-10097113 None:intergenic
ATTCAATTTCCTTCTTTCTA+AGG 0.183402 2:+10097057 MsG0280007063.01.T01:CDS
AAAGAAGGAAATTGAATTTA+GGG 0.185509 2:-10097051 None:intergenic
ATTCAATTCCTGCTTCAAAA+TGG 0.189508 2:+10097265 MsG0280007063.01.T01:CDS
GAGAGTTTAGAAGCAGTTTC+TGG 0.245687 2:-10096884 None:intergenic
GAGATGGGAATTTAGAATAA+AGG 0.311834 2:-10097237 None:intergenic
TTCAATTCCTGCTTCAAAAT+GGG 0.338390 2:+10097266 MsG0280007063.01.T01:CDS
AATAAGGCGATGACTGAATC+AGG 0.360376 2:-10096938 None:intergenic
TTTGATGAGAGATGATAATT+GGG 0.380318 2:-10096984 None:intergenic
GAAATTGAATTTAGGGAGAA+TGG 0.403378 2:-10097044 None:intergenic
TTCAATTTCCTTCTTTCTAA+GGG 0.407285 2:+10097058 MsG0280007063.01.T01:CDS
GAAACCATAGGATTTGAATA+AGG 0.422117 2:-10096954 None:intergenic
AAGCTTTGAGACAACATAAG+AGG 0.427786 2:-10097121 None:intergenic
AACTACCTCATTCAAAATTG+TGG 0.437653 2:+10096854 MsG0280007063.01.T01:CDS
TCTTATGTTGTCTCAAAGCT+TGG 0.442901 2:+10097123 MsG0280007063.01.T01:CDS
ATCGCCTTATTCAAATCCTA+TGG 0.446132 2:+10096950 MsG0280007063.01.T01:CDS
AAGAAGATGAAGAGTTATTG+AGG 0.449182 2:-10096783 None:intergenic
TTGAAGCACCCTTAGAAAGA+AGG 0.450723 2:-10097066 None:intergenic
ACTACCTCATTCAAAATTGT+GGG 0.457092 2:+10096855 MsG0280007063.01.T01:CDS
TCAATTCCTGCTTCAAAATG+GGG 0.519084 2:+10097267 MsG0280007063.01.T01:CDS
ATCTCTCATCAAAACTCGCA+CGG 0.575191 2:+10096994 MsG0280007063.01.T01:CDS
TTTGATAAAATGATAACAAG+GGG 0.581767 2:-10097158 None:intergenic
TTGGGAATTAGAGAAACCAT+AGG 0.600081 2:-10096966 None:intergenic
ACAAGAGCAATGTTTGAGAG+CGG 0.670001 2:-10097212 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGATAAAATGATAACAA+GGG - Chr2:10097162-10097181 None:intergenic 15.0%
!! AAAGAAGGAAATTGAATTTA+GGG - Chr2:10097054-10097073 None:intergenic 20.0%
!! TTTGATAAAATGATAACAAG+GGG - Chr2:10097161-10097180 None:intergenic 20.0%
!!! AAAAAAACAATTTTGGATTC+AGG - Chr2:10097295-10097314 None:intergenic 20.0%
!!! CGAAACAAAAAAAACAATTT+TGG - Chr2:10097302-10097321 None:intergenic 20.0%
!!! CTTTTGATAAAATGATAACA+AGG - Chr2:10097163-10097182 None:intergenic 20.0%
!!! TGTTTTTTTTGTTTCGAAAT+TGG + Chr2:10097306-10097325 MsG0280007063.01.T01:CDS 20.0%
! ATTCAATTTCCTTCTTTCTA+AGG + Chr2:10097057-10097076 MsG0280007063.01.T01:CDS 25.0%
! GAAAGAAGGAAATTGAATTT+AGG - Chr2:10097055-10097074 None:intergenic 25.0%
! TTCAATTTCCTTCTTTCTAA+GGG + Chr2:10097058-10097077 MsG0280007063.01.T01:CDS 25.0%
! TTTGATGAGAGATGATAATT+GGG - Chr2:10096987-10097006 None:intergenic 25.0%
!! TTTTGATGAGAGATGATAAT+TGG - Chr2:10096988-10097007 None:intergenic 25.0%
!!! AGGAATTGAATATTTTGAGA+TGG - Chr2:10097256-10097275 None:intergenic 25.0%
!!! GGAATTGAATATTTTGAGAT+GGG - Chr2:10097255-10097274 None:intergenic 25.0%
AACTACCTCATTCAAAATTG+TGG + Chr2:10096854-10096873 MsG0280007063.01.T01:CDS 30.0%
AAGAAGATGAAGAGTTATTG+AGG - Chr2:10096786-10096805 None:intergenic 30.0%
ACTACCTCATTCAAAATTGT+GGG + Chr2:10096855-10096874 MsG0280007063.01.T01:CDS 30.0%
ATTCAATTCCTGCTTCAAAA+TGG + Chr2:10097265-10097284 MsG0280007063.01.T01:CDS 30.0%
GAAACCATAGGATTTGAATA+AGG - Chr2:10096957-10096976 None:intergenic 30.0%
GAAATTGAATTTAGGGAGAA+TGG - Chr2:10097047-10097066 None:intergenic 30.0%
TTCAATTCCTGCTTCAAAAT+GGG + Chr2:10097266-10097285 MsG0280007063.01.T01:CDS 30.0%
! AAAACCCACAATTTTGAATG+AGG - Chr2:10096862-10096881 None:intergenic 30.0%
! GACTGTAAAAGAGTGATTTT+GGG - Chr2:10096834-10096853 None:intergenic 30.0%
! GAGATGGGAATTTAGAATAA+AGG - Chr2:10097240-10097259 None:intergenic 30.0%
! TGACTGTAAAAGAGTGATTT+TGG - Chr2:10096835-10096854 None:intergenic 30.0%
!!! AGAGTTATTGAGGTTTTGAA+TGG - Chr2:10096776-10096795 None:intergenic 30.0%
!!! GAGTTATTGAGGTTTTGAAT+GGG - Chr2:10096775-10096794 None:intergenic 30.0%
AGACAACATAAGAGGATTTC+TGG - Chr2:10097116-10097135 None:intergenic 35.0%
TCAATTCCTGCTTCAAAATG+GGG + Chr2:10097267-10097286 MsG0280007063.01.T01:CDS 35.0%
TCTTATGTTGTCTCAAAGCT+TGG + Chr2:10097123-10097142 MsG0280007063.01.T01:CDS 35.0%
TTGGGAATTAGAGAAACCAT+AGG - Chr2:10096969-10096988 None:intergenic 35.0%
! ATCGCCTTATTCAAATCCTA+TGG + Chr2:10096950-10096969 MsG0280007063.01.T01:CDS 35.0%
!! AAGCTTTGAGACAACATAAG+AGG - Chr2:10097124-10097143 None:intergenic 35.0%
!!! TATTGAGGTTTTGAATGGGA+GGG - Chr2:10096771-10096790 None:intergenic 35.0%
!!! TTAGGGAGAATGGTTTTGTT+GGG - Chr2:10097037-10097056 None:intergenic 35.0%
!!! TTATTGAGGTTTTGAATGGG+AGG - Chr2:10096772-10096791 None:intergenic 35.0%
!!! TTTAGGGAGAATGGTTTTGT+TGG - Chr2:10097038-10097057 None:intergenic 35.0%
AATAAGGCGATGACTGAATC+AGG - Chr2:10096941-10096960 None:intergenic 40.0%
ACAAGAGCAATGTTTGAGAG+CGG - Chr2:10097215-10097234 None:intergenic 40.0%
ATCTCTCATCAAAACTCGCA+CGG + Chr2:10096994-10097013 MsG0280007063.01.T01:CDS 40.0%
GAGAGTTTAGAAGCAGTTTC+TGG - Chr2:10096887-10096906 None:intergenic 40.0%
! TTGAAGCACCCTTAGAAAGA+AGG - Chr2:10097069-10097088 None:intergenic 40.0%
!!! ATTGAGGTTTTGAATGGGAG+GGG - Chr2:10096770-10096789 None:intergenic 40.0%
! TTTTGAATGGGAGGGGGTTA+GGG - Chr2:10096763-10096782 None:intergenic 45.0%
!!! TTGAGGTTTTGAATGGGAGG+GGG - Chr2:10096769-10096788 None:intergenic 45.0%
!! GTTTTGAATGGGAGGGGGTT+AGG - Chr2:10096764-10096783 None:intergenic 50.0%
!!! CAGGAACCCCATTTTGAAGC+AGG - Chr2:10097276-10097295 None:intergenic 50.0%
AGGGGGTTAGGGCAGAGTAA+TGG - Chr2:10096752-10096771 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 10096728 10097333 10096728 ID=MsG0280007063.01;Name=MsG0280007063.01
Chr2 mRNA 10096728 10097333 10096728 ID=MsG0280007063.01.T01;Parent=MsG0280007063.01;Name=MsG0280007063.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|0|1|0|201
Chr2 exon 10096728 10097333 10096728 ID=MsG0280007063.01.T01:exon:2374;Parent=MsG0280007063.01.T01
Chr2 CDS 10096728 10097333 10096728 ID=MsG0280007063.01.T01:cds;Parent=MsG0280007063.01.T01
Gene Sequence

>MsG0280007063.01.T01

ATGCTTTGTTACTTTCGTAGACCATTACTCTGCCCTAACCCCCTCCCATTCAAAACCTCAATAACTCTTCATCTTCTTCAACTCTCTTCTTCTTCTTCTTCTTCCCAAAATCACTCTTTTACAGTCAACTACCTCATTCAAAATTGTGGGTTTTCACCAGAAACTGCTTCTAAACTCTCTATACGAGTTCATCTCAACAACTCACAAAAACCTGATTCAGTCATCGCCTTATTCAAATCCTATGGTTTCTCTAATTCCCAATTATCATCTCTCATCAAAACTCGCACGGATATTCTCTCTTATGATCCCAACAAAACCATTCTCCCTAAATTCAATTTCCTTCTTTCTAAGGGTGCTTCAAATTCAGATCTTGTTCACATCATCACCAGAAATCCTCTTATGTTGTCTCAAAGCTTGGAAAATACAATAACCCCTTGTTATCATTTTATCAAAAGATTCTTTCTTTCTGATCAATCTACTATTGCCGCTCTCAAACATTGCTCTTGTTTCCTTTATTCTAAATTCCCATCTCAAAATATTCAATTCCTGCTTCAAAATGGGGTTCCTGAATCCAAAATTGTTTTTTTTGTTTCGAAATTGGTATAA
Protein sequence

>MsG0280007063.01.T01

MLCYFRRPLLCPNPLPFKTSITLHLLQLSSSSSSSQNHSFTVNYLIQNCGFSPETASKLSIRVHLNNSQKPDSVIALFKSYGFSNSQLSSLIKTRTDILSYDPNKTILPKFNFLLSKGASNSDLVHIITRNPLMLSQSLENTITPCYHFIKRFFLSDQSTIAALKHCSCFLYSKFPSQNIQFLLQNGVPESKIVFFVSKLV*