AlfalfaGEDB | MsG0280007416.01

M. sativa cultivar ZhongmuNo.1 / MsG0280007416.01

KEGG: -
GO: GO:0003677 GO:0005634 GO:0006355 GO:0009725
PFAM: PF02362 PF06507

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007416.01.T01	XP_013462902.1	88.932	515	54	2	1	512	1	515	0	947

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007416.01.T01	Q84WU6	37.684	544	263	16	9	489	1	531	1.67E-100	317

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007416.01.T01	A0A072V559	88.932	515	54	2	1	512	1	515	0.0	947

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TFs/TRs:

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Gene ID	Type	Classification
MsG0280007416.01.T01	TF	B3-ARF

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007416.01.T01	MTR_2g024430	88.932	515	54	2	1	512	1	515	0.0	947
MsG0280007416.01.T01	MTR_5g040880	59.533	514	190	8	13	511	4	514	0.0	565
MsG0280007416.01.T01	MTR_5g082140	54.167	504	179	7	9	506	1	458	5.46e-178	508
MsG0280007416.01.T01	MTR_5g061220	50.554	542	210	12	7	511	6	526	1.87e-166	481
MsG0280007416.01.T01	MTR_5g040740	53.150	508	130	9	9	511	1	405	1.05e-165	475
MsG0280007416.01.T01	MTR_1g058210	45.686	510	195	14	9	504	1	442	1.15e-132	393
MsG0280007416.01.T01	MTR_2g006270	45.759	507	204	11	13	510	6	450	2.47e-129	384
MsG0280007416.01.T01	MTR_3g073420	43.113	559	251	12	10	511	9	557	3.57e-126	381
MsG0280007416.01.T01	MTR_2g006380	41.980	505	217	15	14	510	14	450	5.89e-112	340
MsG0280007416.01.T01	MTR_5g061890	49.598	373	153	14	9	362	1	357	1.78e-100	308

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007416.01.T01	AT1G77850	37.684	544	263	16	9	489	1	531	1.70e-101	317
MsG0280007416.01.T01	AT1G77850	37.684	544	263	16	9	489	1	531	2.80e-101	317
MsG0280007416.01.T01	AT4G30080	40.376	426	173	14	17	379	16	423	1.27e-83	273
MsG0280007416.01.T01	AT2G28350	38.942	416	177	12	13	363	3	406	2.95e-80	264
MsG0280007416.01.T01	AT2G28350	38.942	416	177	12	13	363	3	406	3.26e-80	263
MsG0280007416.01.T01	AT2G46530	36.240	367	191	16	20	358	21	372	9.83e-55	194
MsG0280007416.01.T01	AT2G46530	36.240	367	191	16	20	358	42	393	1.70e-54	194
MsG0280007416.01.T01	AT3G61830	36.176	340	187	13	20	339	25	354	1.05e-53	191
MsG0280007416.01.T01	AT5G37020	34.091	352	195	10	20	345	22	362	1.13e-51	188
MsG0280007416.01.T01	AT5G37020	34.091	352	195	10	20	345	22	362	1.37e-51	188

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Find 135 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGCTTAGAGTTGCTCA+GGG	0.650442	2:+15106970	MsG0280007416.01.T01:CDS
AAATTAGTTCAACCTCCCAA+GGG	0.635245	2:-15106909	None:intergenic
AACATTAGCACATCTATATT+TGG	0.209645	2:+15107231	MsG0280007416.01.T01:CDS
AACCATCAGTGATGGGAGTT+AGG	0.442469	2:-15105204	None:intergenic
AACTAAAGAATCGGTTATGG+CGG	0.592037	2:+15105621	MsG0280007416.01.T01:CDS
AAGATTTGGCGGTGCTGCGC+CGG	0.479939	2:+15104983	MsG0280007416.01.T01:CDS
AAGGACCGTGACCATCAAGA+AGG	0.601243	2:-15105108	None:intergenic
AAGGGAGAAGATGGTTGTAT+GGG	0.567406	2:+15107411	MsG0280007416.01.T01:CDS
AAGGGATAGGGTTTCAGTGT+TGG	0.458344	2:-15105089	None:intergenic
AATATAGATGTGCTAATGTT+TGG	0.410256	2:-15107228	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACCATTATTAGCATCAGATA+GGG	-	Chr2:15105308-15105327	None:intergenic	30.0%
ATATTATAGGTTAACTGGGA+TGG	+	Chr2:15106856-15106875	MsG0280007416.01.T01:intron	30.0%
ATCCAACAATGTTTGATTCA+AGG	-	Chr2:15107067-15107086	None:intergenic	30.0%
ATCCTTGAATCAAACATTGT+TGG	+	Chr2:15107062-15107081	MsG0280007416.01.T01:CDS	30.0%
ATGAAGATTAACTGGAATTG+TGG	+	Chr2:15105748-15105767	MsG0280007416.01.T01:CDS	30.0%
ATGATGATTCTTCAAAGAGA+TGG	+	Chr2:15105797-15105816	MsG0280007416.01.T01:CDS	30.0%
GATAACTAAAGAATCGGTTA+TGG	+	Chr2:15105618-15105637	MsG0280007416.01.T01:CDS	30.0%
GTACTATTGAAGATGCTTAA+AGG	-	Chr2:15106405-15106424	None:intergenic	30.0%
TGTGGTATGACTCATAAAAA+AGG	-	Chr2:15106448-15106467	None:intergenic	30.0%
TTCAAAATGCTATGGTTAAG+AGG	-	Chr2:15107267-15107286	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	15104926	15107446	15104926	ID=MsG0280007416.01;Name=MsG0280007416.01
Chr2	mRNA	15104926	15107446	15104926	ID=MsG0280007416.01.T01;Parent=MsG0280007416.01;Name=MsG0280007416.01.T01;_AED=0.41;_eAED=0.41;_QI=0\|0\|0\|1\|1\|1\|2\|0\|512
Chr2	exon	15104926	15105882	15104926	ID=MsG0280007416.01.T01:exon:935;Parent=MsG0280007416.01.T01
Chr2	exon	15106865	15107446	15106865	ID=MsG0280007416.01.T01:exon:936;Parent=MsG0280007416.01.T01
Chr2	CDS	15104926	15105882	15104926	ID=MsG0280007416.01.T01:cds;Parent=MsG0280007416.01.T01
Chr2	CDS	15106865	15107446	15106865	ID=MsG0280007416.01.T01:cds;Parent=MsG0280007416.01.T01

Gene Sequence

>MsG0280007416.01.T01

ATGGTAGAACCAAATATTTCCGCCATGTCTCCACCACAACCCAGCCGTGTAGAACCAAAGATTTGGCGGTGCTGCGCCGGACACTCTGTCAAAATTCCTAAACTTCATTCAAAAGTTTACTATTTTCCGCAAGGTCACTTAGAACATGCTTGCCCTAATCCCCCCAACACTGAAACCCTATCCCTTCTTGATGGTCACGGTCCTTTCAATCCTTGCATTGTATCGGCCGTTGATTACCTCGCCGATCCTCATACTGATGAAGTCTTTGTCAAACTTCTCCTAACTCCCATCACTGATGGTTGTGTTCACGAACCTCCTACTGAGGTTCGTGAAGAGGAACCTACAGGTGATGAAATTGTTTCCTCTGGAAAAACTCTAACCCTATCTGATGCTAATAATGGTGGTGCATTCTCCGTACCTCGTTCTTGCGCGACATCAATCTTCCCACCGCTTGACCTCGAAACTAAACAGCCATCTCAAGCACTGTCTGTTACCGACGTTCATGGCAAAGTCTGGGAGTTTCGCCATGTCTACCGTGGAACTCCCTTGAGACACCTGATCACCACTAATTGGAGTAAGTTCGTGGACGAAAAGAAACTCGTCTGCGGCGATTCTGTAATTTTTATGAAAAAGAAATCATCTGGAGATATGTTTGTTGGAATCCGTCGGCATACTAAGTTTGGTGCTGCTAAGATAACTAAAGAATCGGTTATGGCGGCAGCGGAGTTGGCAGATAAGAATATCGCTTTTGAAGTTGAGTATTATCCTACGATTGACGGGTTTTGTGATTTTGTGGTGGATGCCAAGGTTGTTGAGGATGCAATGAAGATTAACTGGAATTGTGGAATGAGAATTAAATTTTCTTTGAAGAATGATGATTCTTCAAAGAGATGGTCAGTTTTTAAGGGTACAATATCTGCTCTCTCTGCTCCTAATCGTCCATGGCGCATGCTTGAGGTTAACTGGGATGGACCTGAAGTCTCACATAATCCAAAGCAAAGCCCTTGGGAGGTTGAACTAATTTCTTATATACCTGCACTGAATCTACAATTGCCCTCAGCAAAAAGGCTTAGAGTTGCTCAGGGTTATACCTCATTAGATAATATAGAGAGATCTTCCATTCCAATGATAGATTTCCAGCATTCAACAGCAAAATCCTTGAATCAAACATTGTTGGATTGTGATACTTTTATTGAGGGCATGCAGAGAGCCCAAGACAGTCTTTTTTCCACAGGAACTACTTCCAACTCTTTCAATGATAGGGCCAATGATTTCATTGAGAAAAATTCAATGGGAAGGTTGGATATTGTGTCAACTGAGCCAAACATTAGCACATCTATATTTGGTGACTTGTCACCTCTTAACCATAGCATTTTGAACCCCTTTGAGACAAAGTTTGACGGTCCCCACAAGTGCAGCACCAAAGAAGTTAATCCTGGTTCATTTATGCTATTTGGTAAGATGATACATCCTGTTGGAAGTAATTTGCATGATTCTGCTACCAAGGGAGAAGATGGTTGTATGGGCTGCAATAATTGA

Protein sequence

>MsG0280007416.01.T01

MVEPNISAMSPPQPSRVEPKIWRCCAGHSVKIPKLHSKVYYFPQGHLEHACPNPPNTETLSLLDGHGPFNPCIVSAVDYLADPHTDEVFVKLLLTPITDGCVHEPPTEVREEEPTGDEIVSSGKTLTLSDANNGGAFSVPRSCATSIFPPLDLETKQPSQALSVTDVHGKVWEFRHVYRGTPLRHLITTNWSKFVDEKKLVCGDSVIFMKKKSSGDMFVGIRRHTKFGAAKITKESVMAAAELADKNIAFEVEYYPTIDGFCDFVVDAKVVEDAMKINWNCGMRIKFSLKNDDSSKRWSVFKGTISALSAPNRPWRMLEVNWDGPEVSHNPKQSPWEVELISYIPALNLQLPSAKRLRVAQGYTSLDNIERSSIPMIDFQHSTAKSLNQTLLDCDTFIEGMQRAQDSLFSTGTTSNSFNDRANDFIEKNSMGRLDIVSTEPNISTSIFGDLSPLNHSILNPFETKFDGPHKCSTKEVNPGSFMLFGKMIHPVGSNLHDSATKGEDGCMGCNN*