AlfalfaGEDB | MsG0280007489.01

M. sativa cultivar ZhongmuNo.1 / MsG0280007489.01

KEGG: -
GO: -
PFAM: PF03514

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007489.01.T01	RHN72665.1	91.743	109	8	1	1	108	319	427	1.09E-62	206

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007489.01.T01	Q9S7H5	58.879	107	43	1	1	106	307	413	1.14E-39	139

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007489.01.T01	A0A396J828	91.743	109	8	1	1	108	319	427	5.18e-63	206

TFs/TRs:

Gene ID	Type	Classification
MsG0280007489.01.T01	TF	GRAS

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007489.01.T01	MTR_2g026250	91.743	109	8	1	1	108	490	598	2.42e-66	210
MsG0280007489.01.T01	MTR_4g133660	62.037	108	40	1	1	107	437	544	1.87e-41	143
MsG0280007489.01.T01	MTR_5g097480	56.075	107	46	1	1	106	438	544	2.33e-39	137
MsG0280007489.01.T01	MTR_3g056110	52.336	107	50	1	1	106	436	542	1.67e-36	129
MsG0280007489.01.T01	MTR_2g082090	54.717	106	47	1	1	105	473	578	5.59e-36	128
MsG0280007489.01.T01	MTR_5g094450	51.402	107	51	1	1	106	426	532	1.82e-35	127
MsG0280007489.01.T01	MTR_1g029420	43.925	107	59	1	1	106	486	592	2.58e-29	110
MsG0280007489.01.T01	MTR_3g089055	43.925	107	59	1	1	106	464	570	2.03e-27	104
MsG0280007489.01.T01	MTR_3g089055	43.925	107	59	1	1	106	459	565	2.10e-27	104
MsG0280007489.01.T01	MTR_7g027190	40.367	109	63	2	1	107	566	674	3.20e-22	90.1
MsG0280007489.01.T01	MTR_4g064160	35.514	107	67	2	1	105	577	683	2.22e-17	76.3
MsG0280007489.01.T01	MTR_4g064200	35.185	108	67	3	1	105	542	649	4.16e-17	75.5
MsG0280007489.01.T01	MTR_4g104020	36.937	111	63	3	1	107	414	521	1.37e-16	73.9
MsG0280007489.01.T01	MTR_4g064150	30.841	107	72	2	1	105	626	732	2.24e-16	73.6
MsG0280007489.01.T01	MTR_7g057230	34.906	106	66	3	1	105	553	656	1.66e-15	71.2
MsG0280007489.01.T01	MTR_2g097410	32.710	107	70	2	1	105	634	740	1.17e-14	68.6
MsG0280007489.01.T01	MTR_2g097473	29.907	107	73	2	1	105	547	653	2.80e-14	67.4
MsG0280007489.01.T01	MTR_5g009080	32.407	108	71	2	1	106	371	478	7.16e-14	66.2
MsG0280007489.01.T01	MTR_6g047750	33.333	102	60	3	12	106	562	662	7.79e-14	66.2
MsG0280007489.01.T01	MTR_8g442410	30.909	110	68	3	1	106	420	525	1.25e-13	65.9
MsG0280007489.01.T01	MTR_4g076140	33.333	108	70	2	1	106	362	469	1.96e-13	65.1
MsG0280007489.01.T01	MTR_2g097467	28.972	107	74	2	1	105	547	653	2.14e-13	65.1
MsG0280007489.01.T01	MTR_4g064120	28.571	105	73	2	1	103	519	623	5.80e-13	63.9
MsG0280007489.01.T01	MTR_2g097350	33.673	98	62	2	11	105	542	639	5.87e-13	63.9
MsG0280007489.01.T01	MTR_2g097463	27.523	109	73	3	1	105	548	654	1.38e-12	62.8
MsG0280007489.01.T01	MTR_4g102790	34.513	113	65	5	1	105	550	661	2.56e-12	62.0
MsG0280007489.01.T01	MTR_7g062120	27.778	108	76	2	1	106	633	740	3.01e-12	61.6
MsG0280007489.01.T01	MTR_5g015490	33.645	107	68	3	2	106	385	490	3.38e-12	61.6
MsG0280007489.01.T01	MTR_2g097310	32.653	98	63	2	11	105	540	637	1.02e-11	60.1
MsG0280007489.01.T01	MTR_3g022830	28.972	107	75	1	1	106	330	436	1.14e-11	60.1
MsG0280007489.01.T01	MTR_4g064180	27.619	105	74	2	1	103	519	623	1.30e-11	60.1
MsG0280007489.01.T01	MTR_4g097080	32.407	108	69	3	2	106	397	503	1.31e-11	60.1
MsG0280007489.01.T01	MTR_2g097390	31.193	109	71	3	1	105	532	640	6.24e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007489.01.T01	AT2G04890	58.879	107	43	1	1	106	307	413	1.16e-40	139
MsG0280007489.01.T01	AT5G48150	57.009	107	45	1	1	106	384	490	7.11e-40	138
MsG0280007489.01.T01	AT5G48150	57.009	107	45	1	1	106	384	490	7.11e-40	138
MsG0280007489.01.T01	AT5G48150	57.009	107	45	1	1	106	384	490	7.11e-40	138
MsG0280007489.01.T01	AT5G48150	57.009	107	45	1	1	106	384	490	7.11e-40	138
MsG0280007489.01.T01	AT1G50600	55.140	107	47	1	1	106	420	526	3.74e-36	128
MsG0280007489.01.T01	AT1G50600	55.140	107	47	1	1	106	491	597	6.18e-36	128
MsG0280007489.01.T01	AT1G50600	55.140	107	47	1	1	106	495	601	6.32e-36	128
MsG0280007489.01.T01	AT4G17230	53.271	107	49	1	1	106	419	525	8.14e-34	122
MsG0280007489.01.T01	AT4G17230	53.271	107	49	1	1	106	419	525	8.14e-34	122
MsG0280007489.01.T01	AT1G21450	44.444	108	58	2	1	106	486	593	2.04e-28	107
MsG0280007489.01.T01	AT5G52510	32.710	107	70	2	1	106	535	640	3.51e-17	75.9
MsG0280007489.01.T01	AT1G50420	36.697	109	67	2	1	107	372	480	9.33e-17	74.7
MsG0280007489.01.T01	AT5G17490	36.937	111	62	5	1	106	409	516	3.79e-16	72.8
MsG0280007489.01.T01	AT3G03450	32.110	109	70	3	1	106	438	545	3.64e-15	70.1
MsG0280007489.01.T01	AT2G01570	32.110	109	68	5	1	106	476	581	6.63e-15	69.3
MsG0280007489.01.T01	AT1G07530	29.907	107	73	2	1	105	658	764	6.72e-15	69.3
MsG0280007489.01.T01	AT1G14920	32.110	109	68	4	1	106	424	529	1.31e-14	68.6
MsG0280007489.01.T01	AT1G66350	32.110	109	67	5	1	106	402	506	5.59e-14	66.6
MsG0280007489.01.T01	AT2G29060	29.730	111	69	3	1	105	547	654	1.76e-12	62.4
MsG0280007489.01.T01	AT2G29065	31.481	108	71	2	1	105	520	627	1.88e-12	62.4
MsG0280007489.01.T01	AT2G29060	29.730	111	69	3	1	105	584	691	1.93e-12	62.4
MsG0280007489.01.T01	AT2G37650	28.704	108	75	2	1	106	594	701	8.89e-12	60.5
MsG0280007489.01.T01	AT2G37650	28.704	108	75	2	1	106	606	713	9.44e-12	60.5
MsG0280007489.01.T01	AT1G07520	27.778	108	75	2	1	105	586	693	1.12e-11	60.1
MsG0280007489.01.T01	AT3G46600	29.630	108	74	2	1	106	264	371	1.14e-11	60.1
MsG0280007489.01.T01	AT1G07520	27.778	108	75	2	1	105	585	692	1.15e-11	60.1
MsG0280007489.01.T01	AT1G07520	27.778	108	75	2	1	105	421	528	1.49e-11	59.7
MsG0280007489.01.T01	AT1G07520	27.778	108	75	2	1	105	421	528	1.49e-11	59.7
MsG0280007489.01.T01	AT3G46600	29.630	108	74	2	1	106	472	579	1.69e-11	59.7
MsG0280007489.01.T01	AT3G46600	29.630	108	74	2	1	106	440	547	1.80e-11	59.7
MsG0280007489.01.T01	AT1G55580	26.126	111	75	3	1	106	337	445	4.54e-11	58.5

Find 15 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGATTTCAATAAAGATTAT+AGG	0.216624	2:-16134690	MsG0280007489.01.T01:CDS
ATTGAAAGACATGAACTGTT+TGG	0.379235	2:-16134796	MsG0280007489.01.T01:CDS
TTATGTGCACATCTTCTGCT+TGG	0.493993	2:-16134621	MsG0280007489.01.T01:CDS
ATTCTCTTCTTATCATCTCT+AGG	0.523465	2:+16134886	None:intergenic
GACATGAACTGTTTGGAAAG+TGG	0.527553	2:-16134789	MsG0280007489.01.T01:CDS
TCAATCACCGAGGTACTCAA+TGG	0.553423	2:+16134733	None:intergenic
ACCGAGGTACTCAATGGTAA+TGG	0.566617	2:+16134739	None:intergenic
CGTTAACATCATAGCTTGTG+AGG	0.593625	2:-16134830	MsG0280007489.01.T01:CDS
GTTAACATCATAGCTTGTGA+GGG	0.618537	2:-16134829	MsG0280007489.01.T01:CDS
ACCATTACCATTGAGTACCT+CGG	0.650766	2:-16134740	MsG0280007489.01.T01:CDS
ATGAACTGTTTGGAAAGTGG+AGG	0.656082	2:-16134786	MsG0280007489.01.T01:CDS
ATGTTGCTATCAATTTAGCG+TGG	0.662028	2:-16134654	MsG0280007489.01.T01:CDS
AATGTTTGAATCAATTGATG+TGG	0.662206	2:-16134914	MsG0280007489.01.T01:CDS
TAATGCAGAACAACACTGTG+TGG	0.712966	2:-16134863	MsG0280007489.01.T01:CDS
TCTAACTGAATCAATCACCG+AGG	0.797585	2:+16134723	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGATTTCAATAAAGATTAT+AGG	-	Chr2:16134832-16134851	MsG0280007489.01.T01:CDS	15.0%
	ATTCTCTTCTTATCATCTCT+AGG	+	Chr2:16134639-16134658	None:intergenic	30.0%
	ATTGAAAGACATGAACTGTT+TGG	-	Chr2:16134726-16134745	MsG0280007489.01.T01:CDS	30.0%
	ATGTTGCTATCAATTTAGCG+TGG	-	Chr2:16134868-16134887	MsG0280007489.01.T01:CDS	35.0%
!	AGTGGAGGAAAAGATTTTCA+TGG	-	Chr2:16134751-16134770	MsG0280007489.01.T01:CDS	35.0%
!	GTTAACATCATAGCTTGTGA+GGG	-	Chr2:16134693-16134712	MsG0280007489.01.T01:CDS	35.0%
	ACCATTACCATTGAGTACCT+CGG	-	Chr2:16134782-16134801	MsG0280007489.01.T01:CDS	40.0%
	ATGAACTGTTTGGAAAGTGG+AGG	-	Chr2:16134736-16134755	MsG0280007489.01.T01:CDS	40.0%
	GACATGAACTGTTTGGAAAG+TGG	-	Chr2:16134733-16134752	MsG0280007489.01.T01:CDS	40.0%
	TAATGCAGAACAACACTGTG+TGG	-	Chr2:16134659-16134678	MsG0280007489.01.T01:CDS	40.0%
	TCTAACTGAATCAATCACCG+AGG	+	Chr2:16134802-16134821	None:intergenic	40.0%
	TTATGTGCACATCTTCTGCT+TGG	-	Chr2:16134901-16134920	MsG0280007489.01.T01:CDS	40.0%
!	CGTTAACATCATAGCTTGTG+AGG	-	Chr2:16134692-16134711	MsG0280007489.01.T01:CDS	40.0%
!	GGAGGAAAAGATTTTCATGG+CGG	-	Chr2:16134754-16134773	MsG0280007489.01.T01:CDS	40.0%
!	ACCGAGGTACTCAATGGTAA+TGG	+	Chr2:16134786-16134805	None:intergenic	45.0%
!	TCAATCACCGAGGTACTCAA+TGG	+	Chr2:16134792-16134811	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	16134609	16134935	16134609	ID=MsG0280007489.01;Name=MsG0280007489.01
Chr2	mRNA	16134609	16134935	16134609	ID=MsG0280007489.01.T01;Parent=MsG0280007489.01;Name=MsG0280007489.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|108
Chr2	exon	16134609	16134935	16134609	ID=MsG0280007489.01.T01:exon:4283;Parent=MsG0280007489.01.T01
Chr2	CDS	16134609	16134935	16134609	ID=MsG0280007489.01.T01:cds;Parent=MsG0280007489.01.T01
Chr2	mRNA	16134839	16134919	16134839	ID=MsG0280007489.01.T02;Parent=MsG0280007489.01;Name=MsG0280007489.01.T02;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|26
Chr2	exon	16134839	16134919	16134839	ID=MsG0280007489.01.T02:exon:4284;Parent=MsG0280007489.01.T02
Chr2	CDS	16134839	16134919	16134839	ID=MsG0280007489.01.T02:cds;Parent=MsG0280007489.01.T02

Gene Sequence

>MsG0280007489.01.T02

ATGTGGCTCTTCCTAGAGATGATAAGAAGAGAATTAATGCAGAACAACACTGTGTGGCTCGCGATATCGTTAACATCATAG

>MsG0280007489.01.T01

ATGTTTGAATCAATTGATGTGGCTCTTCCTAGAGATGATAAGAAGAGAATTAATGCAGAACAACACTGTGTGGCTCGCGATATCGTTAACATCATAGCTTGTGAGGGAGATGAGAGAATTGAAAGACATGAACTGTTTGGAAAGTGGAGGAAAAGATTTTCATGGCGGTTCGTACCATTACCATTGAGTACCTCGGTGATTGATTCAGTTAGAACTCTCTTGAAAGATTTCAATAAAGATTATAGGATTGAAGAAACAGATGTTGCTATCAATTTAGCGTGGAAGAGTAAAGTTATGTGCACATCTTCTGCTTGGAGATGTTATTGA

Protein sequence

>MsG0280007489.01.T02

MWLFLEMIRRELMQNNTVWLAISLTS*

>MsG0280007489.01.T01

MFESIDVALPRDDKKRINAEQHCVARDIVNIIACEGDERIERHELFGKWRKRFSWRFVPLPLSTSVIDSVRTLLKDFNKDYRIEETDVAINLAWKSKVMCTSSAWRCY*