AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007535.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007535.01.T01 MTR_2g027240 63.333 150 22 2 1 117 1 150 9.11e-42 150
MsG0280007535.01.T01 MTR_2g027240 62.921 89 17 2 152 224 495 583 8.29e-27 108
MsG0280007535.01.T01 MTR_2g027240 51.807 83 34 3 119 199 311 389 6.38e-18 82.4
MsG0280007535.01.T01 MTR_8g006870 68.085 47 15 0 112 158 470 516 8.88e-16 76.3
MsG0280007535.01.T01 MTR_8g006870 85.714 28 4 0 174 201 693 720 3.04e-11 63.2
MsG0280007535.01.T01 MTR_8g446990 94.286 35 2 0 124 158 244 278 9.54e-16 76.3
MsG0280007535.01.T01 MTR_8g446990 54.717 53 22 1 174 224 455 507 3.20e-11 62.8
MsG0280007535.01.T01 MTR_1g060790 57.812 64 19 3 119 179 577 635 1.85e-15 75.5
MsG0280007535.01.T01 MTR_1g060790 47.945 73 22 2 152 208 755 827 1.87e-14 72.4
MsG0280007535.01.T01 MTR_7g033640 77.500 40 9 0 119 158 339 378 1.86e-15 75.5
MsG0280007535.01.T01 MTR_7g033640 76.667 30 7 0 174 203 561 590 2.63e-11 63.2
MsG0280007535.01.T01 MTR_6g004720 77.500 40 9 0 119 158 358 397 2.22e-15 75.1
MsG0280007535.01.T01 MTR_7g081665 65.957 47 16 0 112 158 464 510 2.41e-15 75.1
MsG0280007535.01.T01 MTR_7g081665 77.143 35 8 0 174 208 686 720 2.29e-13 69.3
MsG0280007535.01.T01 MTR_7g104180 72.500 40 11 0 119 158 599 638 3.16e-15 74.7
MsG0280007535.01.T01 MTR_7g104180 44.444 72 24 2 152 207 777 848 7.03e-13 67.8
MsG0280007535.01.T01 MTR_2g062850 88.571 35 4 0 124 158 281 315 3.52e-15 74.7
MsG0280007535.01.T01 MTR_2g062850 75.000 36 9 0 174 209 492 527 3.10e-13 68.9
MsG0280007535.01.T01 MTR_8g089420 77.500 40 9 0 119 158 190 229 3.86e-15 74.3
MsG0280007535.01.T01 MTR_3g010260 70.000 40 12 0 119 158 522 561 6.16e-15 73.9
MsG0280007535.01.T01 MTR_3g010260 55.738 61 24 2 173 230 739 799 2.41e-13 69.3
MsG0280007535.01.T01 MTR_4g113790 77.500 40 9 0 119 158 203 242 6.63e-15 73.6
MsG0280007535.01.T01 MTR_2g018990 75.000 40 10 0 119 158 194 233 1.09e-14 72.8
MsG0280007535.01.T01 MTR_2g085065 85.714 35 5 0 124 158 204 238 3.05e-14 71.6
MsG0280007535.01.T01 MTR_2g069990 65.909 44 15 0 119 162 201 244 1.30e-13 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007535.01.T01 AT5G47750 52.500 80 35 2 119 198 296 372 9.32e-17 79.3
MsG0280007535.01.T01 AT5G47750 86.111 36 5 0 174 209 516 551 1.04e-15 76.3
MsG0280007535.01.T01 AT5G55910 51.351 74 27 2 119 183 214 287 2.31e-16 77.8
MsG0280007535.01.T01 AT5G55910 62.745 51 18 1 174 223 435 485 8.24e-16 76.3
MsG0280007535.01.T01 AT5G55910 51.351 74 27 2 119 183 214 287 2.31e-16 77.8
MsG0280007535.01.T01 AT5G55910 62.745 51 18 1 174 223 435 485 8.24e-16 76.3
MsG0280007535.01.T01 AT1G79250 85.000 40 6 0 119 158 251 290 2.33e-16 78.2
MsG0280007535.01.T01 AT1G79250 76.471 34 8 0 174 207 469 502 3.22e-12 65.9
MsG0280007535.01.T01 AT1G79250 85.000 40 6 0 119 158 251 290 2.33e-16 78.2
MsG0280007535.01.T01 AT1G79250 76.471 34 8 0 174 207 469 502 3.22e-12 65.9
MsG0280007535.01.T01 AT1G79250 85.000 40 6 0 119 158 251 290 2.33e-16 78.2
MsG0280007535.01.T01 AT1G79250 76.471 34 8 0 174 207 469 502 3.22e-12 65.9
MsG0280007535.01.T01 AT1G79250 85.000 40 6 0 119 158 251 290 2.33e-16 78.2
MsG0280007535.01.T01 AT1G79250 76.471 34 8 0 174 207 469 502 3.22e-12 65.9
MsG0280007535.01.T01 AT4G26610 80.000 40 8 0 119 158 228 267 2.40e-16 77.8
MsG0280007535.01.T01 AT4G26610 77.143 35 8 0 174 208 445 479 4.58e-14 71.2
MsG0280007535.01.T01 AT1G16440 85.000 40 6 0 119 158 220 259 2.77e-16 77.8
MsG0280007535.01.T01 AT2G44830 80.000 40 8 0 119 158 468 507 7.33e-16 76.6
MsG0280007535.01.T01 AT2G44830 73.684 38 10 0 174 211 686 723 6.49e-14 70.9
MsG0280007535.01.T01 AT2G44830 80.000 40 8 0 119 158 468 507 7.33e-16 76.6
MsG0280007535.01.T01 AT2G44830 73.684 38 10 0 174 211 686 723 6.49e-14 70.9
MsG0280007535.01.T01 AT2G44830 80.000 40 8 0 119 158 468 507 7.33e-16 76.6
MsG0280007535.01.T01 AT2G44830 73.684 38 10 0 174 211 686 723 6.49e-14 70.9
MsG0280007535.01.T01 AT3G27580 80.000 40 8 0 119 158 287 326 8.42e-16 76.3
MsG0280007535.01.T01 AT3G27580 80.000 40 8 0 119 158 287 326 8.42e-16 76.3
MsG0280007535.01.T01 AT2G36350 47.561 82 21 3 119 200 664 723 1.89e-15 75.5
MsG0280007535.01.T01 AT2G36350 46.377 69 33 2 135 202 850 915 6.11e-11 62.4
MsG0280007535.01.T01 AT2G26700 65.306 49 15 1 119 165 194 242 3.71e-15 74.3
MsG0280007535.01.T01 AT3G52890 72.500 40 11 0 119 158 643 682 4.16e-15 74.7
MsG0280007535.01.T01 AT3G52890 42.857 84 32 2 152 219 830 913 4.88e-14 71.2
MsG0280007535.01.T01 AT3G52890 72.500 40 11 0 119 158 643 682 4.16e-15 74.7
MsG0280007535.01.T01 AT3G52890 42.857 84 32 2 152 219 830 913 4.88e-14 71.2
MsG0280007535.01.T01 AT3G52890 72.500 40 11 0 119 158 643 682 4.16e-15 74.7
MsG0280007535.01.T01 AT3G52890 42.857 84 32 2 152 219 830 913 4.88e-14 71.2
MsG0280007535.01.T01 AT3G52890 72.500 40 11 0 119 158 643 682 4.16e-15 74.7
MsG0280007535.01.T01 AT3G52890 42.857 84 32 2 152 219 830 913 4.88e-14 71.2
MsG0280007535.01.T01 AT5G03640 70.000 40 12 0 119 158 646 685 5.89e-15 73.9
MsG0280007535.01.T01 AT5G03640 70.000 40 12 0 119 158 646 685 5.89e-15 73.9
MsG0280007535.01.T01 AT3G12690 75.000 40 10 0 119 158 290 329 7.98e-15 73.6
MsG0280007535.01.T01 AT3G12690 75.000 40 10 0 119 158 290 329 7.98e-15 73.6
MsG0280007535.01.T01 AT3G12690 75.000 40 10 0 119 158 290 329 7.98e-15 73.6
MsG0280007535.01.T01 AT2G34650 65.385 52 13 2 119 170 185 231 1.22e-14 72.8
MsG0280007535.01.T01 AT5G40030 82.857 35 6 0 124 158 224 258 1.22e-14 72.8
MsG0280007535.01.T01 AT1G53700 67.347 49 14 1 119 165 199 247 8.12e-14 70.5
MsG0280007535.01.T01 AT3G44610 82.857 35 6 0 124 158 189 223 9.49e-14 70.1
MsG0280007535.01.T01 AT3G14370 65.909 44 15 0 119 162 193 236 1.59e-13 69.7

Find 42 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTCAGAACCTTTCTGATT+AGG 0.293830 2:+16751645 None:intergenic
AGCCAGAAAATGTTCTTGTT+AGG 0.303331 2:-16752399 MsG0280007535.01.T01:CDS
AGACAAAAGACTTCAGAATA+TGG 0.321838 2:-16753947 MsG0280007535.01.T01:CDS
TTCTCAAACTCAACAGCCTT+TGG 0.340147 2:+16751731 None:intergenic
AATGTTCTTGTTAGGGATGA+TGG 0.383801 2:-16752391 MsG0280007535.01.T01:CDS
GCCAGAAAATGTTCTTGTTA+GGG 0.404235 2:-16752398 MsG0280007535.01.T01:CDS
TCCCCAGCTGCATCGTCTGC+TGG 0.405197 2:-16751701 MsG0280007535.01.T01:CDS
ACAGAGTTAGACAAATCTTC+AGG 0.435268 2:+16754135 None:intergenic
TCGCTGTCGCTTCAAAAGAC+AGG 0.447517 2:-16754168 MsG0280007535.01.T01:CDS
TCAACAGCCTTTGGAATCTC+AGG 0.460268 2:+16751740 None:intergenic
TCGCCAGCAGACGATGCAGC+TGG 0.463955 2:+16751698 None:intergenic
TCGCCTCAGTGTTTGAATCC+CGG 0.465949 2:+16754208 None:intergenic
AAAGGGATGTTGTTTAATCT+CGG 0.474175 2:+16751805 None:intergenic
TGCATCGTCTGCTGGCGATA+AGG 0.490225 2:-16751693 MsG0280007535.01.T01:CDS
AAAGGAACCTCAGCACAGAT+TGG 0.496667 2:-16751844 MsG0280007535.01.T01:intron
CGCCAGCAGACGATGCAGCT+GGG 0.501809 2:+16751699 None:intergenic
CACAGATTGGCTTATAAAAG+AGG 0.508384 2:-16751831 MsG0280007535.01.T01:CDS
ACTTGTCTTCGCAATACTGC+TGG 0.510223 2:+16753880 None:intergenic
CGCTGTCGCTTCAAAAGACA+GGG 0.521858 2:-16754167 MsG0280007535.01.T01:CDS
TGGAGTACCTACACATGCTC+GGG 0.541979 2:-16752441 MsG0280007535.01.T01:CDS
GTGGCAGAGGTTACTGGCAA+TGG 0.545752 2:-16752461 MsG0280007535.01.T01:CDS
AGCAGTATTGCGAAGACAAG+TGG 0.550813 2:-16753878 MsG0280007535.01.T01:CDS
TGGTGTAGCACATCGGATCA+AGG 0.554118 2:+16751763 None:intergenic
ATGGAGTACCTACACATGCT+CGG 0.560351 2:-16752442 MsG0280007535.01.T01:CDS
TTATAAGCCAATCTGTGCTG+AGG 0.571793 2:+16751837 None:intergenic
GCAGACGATGCAGCTGGGGA+AGG 0.572018 2:+16751704 None:intergenic
TCAACAACTCCTAATCAGAA+AGG 0.579889 2:-16751654 None:intergenic
CACTCTCAACAAAATCAGCT+CGG 0.581324 2:+16753837 None:intergenic
TTGGACTAACAGCACATCGA+AGG 0.590565 2:+16752339 None:intergenic
TAACAGCACATCGAAGGGAA+AGG 0.614273 2:+16752345 None:intergenic
GCCAGCAGACGATGCAGCTG+GGG 0.614968 2:+16751700 None:intergenic
GTTTCTGCGAAAGCTAGCGA+TGG 0.621392 2:-16753905 MsG0280007535.01.T01:CDS
GATTAGGAGTTGTTGACATG+TGG 0.625031 2:+16751661 None:intergenic
ACAGCCTTTGGAATCTCAGG+TGG 0.625096 2:+16751743 None:intergenic
TGGACTAACAGCACATCGAA+GGG 0.627323 2:+16752340 None:intergenic
ATCGAAGGGAAAGGTCAAGT+CGG 0.628525 2:+16752354 None:intergenic
ATACAATCCCGAGCATGTGT+AGG 0.645724 2:+16752434 None:intergenic
TTGTTGACATGTGGTTACAA+CGG 0.650745 2:+16751670 None:intergenic
GCGACAGCGAAGAAAACTTG+AGG 0.651781 2:+16754181 None:intergenic
TCTCAGGTGGTGTAGCACAT+CGG 0.670407 2:+16751756 None:intergenic
TACACCACCTGAGATTCCAA+AGG 0.680244 2:-16751747 MsG0280007535.01.T01:CDS
CATCCGGGATTCAAACACTG+AGG 0.759613 2:-16754211 MsG0280007535.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGATGTTAATTTAATTTGA+TGG - Chr2:16753394-16753413 MsG0280007535.01.T01:intron 15.0%
!!! ATTTATTGTAGTATTGAATT+GGG - Chr2:16752720-16752739 MsG0280007535.01.T01:intron 15.0%
!!! TATTTATTGTAGTATTGAAT+TGG - Chr2:16752719-16752738 MsG0280007535.01.T01:intron 15.0%
!! ATTCAATTGGATATTTAACT+GGG - Chr2:16752905-16752924 MsG0280007535.01.T01:intron 20.0%
!! GAAAATATGAAAGTAAAGTA+GGG - Chr2:16751832-16751851 MsG0280007535.01.T01:CDS 20.0%
!! TATAAGTTGCATCATTTAAT+TGG + Chr2:16752701-16752720 None:intergenic 20.0%
!!! ATTTTGTTTGATTAAGAGTA+GGG - Chr2:16752867-16752886 MsG0280007535.01.T01:intron 20.0%
!!! TATTTTGTTTGATTAAGAGT+AGG - Chr2:16752866-16752885 MsG0280007535.01.T01:intron 20.0%
! AACTCTTTGAATGTGTATAT+TGG - Chr2:16752947-16752966 MsG0280007535.01.T01:intron 25.0%
! ATCTGTTTAATCTCATTTAC+TGG - Chr2:16753286-16753305 MsG0280007535.01.T01:intron 25.0%
! ATTATTTGAAAGCCAATTGA+AGG + Chr2:16752663-16752682 None:intergenic 25.0%
! CAATCAAAGAAATCATCATT+AGG + Chr2:16753367-16753386 None:intergenic 25.0%
! CGAAAATATGAAAGTAAAGT+AGG - Chr2:16751831-16751850 MsG0280007535.01.T01:CDS 25.0%
! GATTCAATTGGATATTTAAC+TGG - Chr2:16752904-16752923 MsG0280007535.01.T01:intron 25.0%
! TAAATCATTGCATCTGAATT+TGG + Chr2:16752802-16752821 None:intergenic 25.0%
! TAACCAGTATTCTTTCTTAA+AGG + Chr2:16753634-16753653 None:intergenic 25.0%
! TAGAACAAGAGTTAAAACTA+TGG + Chr2:16752621-16752640 None:intergenic 25.0%
! TCTGTTTAATCTCATTTACT+GGG - Chr2:16753287-16753306 MsG0280007535.01.T01:intron 25.0%
!! AAAATCTTTGAAAGCTTGTT+TGG - Chr2:16753209-16753228 MsG0280007535.01.T01:intron 25.0%
!! AAATCTTTGAAAGCTTGTTT+GGG - Chr2:16753210-16753229 MsG0280007535.01.T01:intron 25.0%
!! AGTATTGAATTGGGAAAAAA+GGG - Chr2:16752729-16752748 MsG0280007535.01.T01:intron 25.0%
!! TAGTATTGAATTGGGAAAAA+AGG - Chr2:16752728-16752747 MsG0280007535.01.T01:intron 25.0%
AACAACATCAGAAAAACATC+CGG - Chr2:16751689-16751708 MsG0280007535.01.T01:CDS 30.0%
AACTATGGAAGGAAATCAAA+AGG + Chr2:16752606-16752625 None:intergenic 30.0%
ACAAAGCAAATGAATGAGAT+GGG - Chr2:16752192-16752211 MsG0280007535.01.T01:intron 30.0%
ACAAGAGTTAAAACTATGGA+AGG + Chr2:16752617-16752636 None:intergenic 30.0%
AGACAAAAGACTTCAGAATA+TGG - Chr2:16751969-16751988 MsG0280007535.01.T01:intron 30.0%
ATTTGCTTGAAACCTTCAAT+TGG - Chr2:16752648-16752667 MsG0280007535.01.T01:intron 30.0%
CACTATAATTGACAACTCTT+GGG - Chr2:16753262-16753281 MsG0280007535.01.T01:intron 30.0%
GATTCACTGAGTAGTAAATT+CGG - Chr2:16751882-16751901 MsG0280007535.01.T01:intron 30.0%
GCAATGATTTAAACCATGTA+TGG - Chr2:16752811-16752830 MsG0280007535.01.T01:intron 30.0%
TCAAAATGAGTGTACAAAGT+TGG + Chr2:16752446-16752465 None:intergenic 30.0%
! AAAGGGATGTTGTTTAATCT+CGG + Chr2:16754114-16754133 None:intergenic 30.0%
! AATCTTTGAAAGCTTGTTTG+GGG - Chr2:16753211-16753230 MsG0280007535.01.T01:intron 30.0%
! GTATATTGGCAATTTTGCAA+TGG - Chr2:16752961-16752980 MsG0280007535.01.T01:intron 30.0%
! GTTTGATATCTGAAATTGCT+TGG + Chr2:16753161-16753180 None:intergenic 30.0%
! TACAATATTTGACTTCATGC+AGG + Chr2:16753132-16753151 None:intergenic 30.0%
! TTTCTATACGAGTTGTTGTT+TGG - Chr2:16753925-16753944 MsG0280007535.01.T01:CDS 30.0%
!! TATAACTTGCAGATTTTACG+TGG - Chr2:16753436-16753455 MsG0280007535.01.T01:intron 30.0%
!! TGTATCTAAGCTTTGAGAAT+GGG - Chr2:16753050-16753069 MsG0280007535.01.T01:intron 30.0%
!! TTTGTACACTCATTTTGAGA+CGG - Chr2:16752448-16752467 MsG0280007535.01.T01:CDS 30.0%
!!! AAACAACATCCCTTTTTTGA+AGG - Chr2:16754119-16754138 MsG0280007535.01.T01:CDS 30.0%
AATGTTCTTGTTAGGGATGA+TGG - Chr2:16753525-16753544 MsG0280007535.01.T01:intron 35.0%
ACAACATCAGAAAAACATCC+GGG - Chr2:16751690-16751709 MsG0280007535.01.T01:CDS 35.0%
ACACTTGCATGTAATTGCTT+TGG - Chr2:16753078-16753097 MsG0280007535.01.T01:intron 35.0%
ACAGAGTTAGACAAATCTTC+AGG + Chr2:16751784-16751803 None:intergenic 35.0%
AGAACTAGAGATGGTTCATT+GGG - Chr2:16752232-16752251 MsG0280007535.01.T01:intron 35.0%
AGAGTGTAACAAGACTATGA+AGG + Chr2:16753005-16753024 None:intergenic 35.0%
AGCCAGAAAATGTTCTTGTT+AGG - Chr2:16753517-16753536 MsG0280007535.01.T01:intron 35.0%
AGTGTACAAAGTTGGAGAAA+CGG + Chr2:16752438-16752457 None:intergenic 35.0%
CAAATCAAAGAAAGAACGCA+AGG - Chr2:16753745-16753764 MsG0280007535.01.T01:intron 35.0%
CAATTCACGCCTTCAAAAAA+GGG + Chr2:16754131-16754150 None:intergenic 35.0%
CACAAAGCAAATGAATGAGA+TGG - Chr2:16752191-16752210 MsG0280007535.01.T01:intron 35.0%
CCAAGAGTTGTCAATTATAG+TGG + Chr2:16753264-16753283 None:intergenic 35.0%
CCACTATAATTGACAACTCT+TGG - Chr2:16753261-16753280 MsG0280007535.01.T01:intron 35.0%
CCTGTGTTTCACATTAACAA+CGG + Chr2:16751925-16751944 None:intergenic 35.0%
GCCAGAAAATGTTCTTGTTA+GGG - Chr2:16753518-16753537 MsG0280007535.01.T01:intron 35.0%
GTAACAGTTCATGTGAAATC+TGG - Chr2:16752775-16752794 MsG0280007535.01.T01:intron 35.0%
GTTTGATTAAGAGTAGGGTA+GGG - Chr2:16752872-16752891 MsG0280007535.01.T01:intron 35.0%
TAGAACTAGAGATGGTTCAT+TGG - Chr2:16752231-16752250 MsG0280007535.01.T01:intron 35.0%
TCTCATTCATTTGCTTTGTG+AGG + Chr2:16752191-16752210 None:intergenic 35.0%
TGTTTGATTAAGAGTAGGGT+AGG - Chr2:16752871-16752890 MsG0280007535.01.T01:intron 35.0%
TTACTTCGCAATGAAAGTGA+TGG - Chr2:16752336-16752355 MsG0280007535.01.T01:intron 35.0%
TTGTTGACATGTGGTTACAA+CGG + Chr2:16754249-16754268 None:intergenic 35.0%
TTTCACATTAACAACGGATG+AGG + Chr2:16751919-16751938 None:intergenic 35.0%
TTTGATTAAGAGTAGGGTAG+GGG - Chr2:16752873-16752892 MsG0280007535.01.T01:intron 35.0%
TTTGGCAAAACTCCGTTTAA+GGG - Chr2:16753943-16753962 MsG0280007535.01.T01:CDS 35.0%
! ACACATTCAAAGAGTTTTGC+AGG + Chr2:16752943-16752962 None:intergenic 35.0%
! ATCTTTGAAAGCTTGTTTGG+GGG - Chr2:16753212-16753231 MsG0280007535.01.T01:intron 35.0%
! CACATTCAAAGAGTTTTGCA+GGG + Chr2:16752942-16752961 None:intergenic 35.0%
! GAGCCTTTAAGAAAGAATAC+TGG - Chr2:16753628-16753647 MsG0280007535.01.T01:intron 35.0%
! TCCCTAACAAGAACATTTTC+TGG + Chr2:16753522-16753541 None:intergenic 35.0%
!! AAAAGGGTGTCATGTATTTG+TGG - Chr2:16752745-16752764 MsG0280007535.01.T01:intron 35.0%
!! CAATGAAAGTGATGGACAAA+GGG - Chr2:16752344-16752363 MsG0280007535.01.T01:intron 35.0%
!! CACAGATTGGCTTATAAAAG+AGG - Chr2:16754085-16754104 MsG0280007535.01.T01:intron 35.0%
!! CTGTATCTAAGCTTTGAGAA+TGG - Chr2:16753049-16753068 MsG0280007535.01.T01:intron 35.0%
!! TTGATTTGCTAGAAAAGAGC+CGG + Chr2:16753733-16753752 None:intergenic 35.0%
!!! CATTTTCTGGCTTAAGATCA+CGG + Chr2:16753509-16753528 None:intergenic 35.0%
!!! CTTTTTTGAAGGCGTGAATT+GGG - Chr2:16754130-16754149 MsG0280007535.01.T01:CDS 35.0%
AAAACTCCGTTTAAGGGATC+CGG - Chr2:16753949-16753968 MsG0280007535.01.T01:CDS 40.0%
AACCATGTATGGTTCGAATG+AGG - Chr2:16752822-16752841 MsG0280007535.01.T01:intron 40.0%
AGAGATGGTTCATTGGGTTT+AGG - Chr2:16752238-16752257 MsG0280007535.01.T01:intron 40.0%
ATAATCAAGGGTGAAGGTCA+CGG - Chr2:16753872-16753891 MsG0280007535.01.T01:CDS 40.0%
CAAGTGGTTAGAACTAGAGA+TGG - Chr2:16752223-16752242 MsG0280007535.01.T01:intron 40.0%
CAATCGACGAGGATTTAACT+AGG + Chr2:16753603-16753622 None:intergenic 40.0%
CACTCTCAACAAAATCAGCT+CGG + Chr2:16752082-16752101 None:intergenic 40.0%
CCAATTCACGCCTTCAAAAA+AGG + Chr2:16754132-16754151 None:intergenic 40.0%
CCGTTGTTAATGTGAAACAC+AGG - Chr2:16751922-16751941 MsG0280007535.01.T01:intron 40.0%
CCTGAAATAATCAAGGGTGA+AGG - Chr2:16753866-16753885 MsG0280007535.01.T01:CDS 40.0%
CCTTCACCCTTGATTATTTC+AGG + Chr2:16753869-16753888 None:intergenic 40.0%
CTGAGTAGTAAATTCGGTTC+TGG - Chr2:16751888-16751907 MsG0280007535.01.T01:intron 40.0%
CTGGCACCTGAAATAATCAA+GGG - Chr2:16753860-16753879 MsG0280007535.01.T01:CDS 40.0%
CTTCATGCAGGACTCAAAAA+AGG + Chr2:16753120-16753139 None:intergenic 40.0%
GATTAGGAGTTGTTGACATG+TGG + Chr2:16754258-16754277 None:intergenic 40.0%
GTGTACAAAGTTGGAGAAAC+GGG + Chr2:16752437-16752456 None:intergenic 40.0%
GTTTGGCAAAACTCCGTTTA+AGG - Chr2:16753942-16753961 MsG0280007535.01.T01:CDS 40.0%
TACCACTTAAACATGTGGCT+CGG + Chr2:16753978-16753997 None:intergenic 40.0%
TGACCTACCACTTAAACATG+TGG + Chr2:16753983-16754002 None:intergenic 40.0%
TTATAAGCCAATCTGTGCTG+AGG + Chr2:16754082-16754101 None:intergenic 40.0%
TTCTCAAACTCAACAGCCTT+TGG + Chr2:16754188-16754207 None:intergenic 40.0%
TTGTTGGAACTCATGATACC+TGG - Chr2:16753841-16753860 MsG0280007535.01.T01:CDS 40.0%
! ACTTGATTGCCAGTTCGTTT+TGG + Chr2:16753771-16753790 None:intergenic 40.0%
! AGAAATTTCGTGCTTGGTGA+TGG - Chr2:16752473-16752492 MsG0280007535.01.T01:intron 40.0%
! GACTATGAAGGTACAGAAAC+AGG + Chr2:16752993-16753012 None:intergenic 40.0%
! GAGTTTTGCAGGGAAAGTTT+TGG + Chr2:16752932-16752951 None:intergenic 40.0%
! GATATCTGAAATTGCTTGGC+AGG + Chr2:16753157-16753176 None:intergenic 40.0%
! TATCAGGGGTTTGCTTGTAA+AGG - Chr2:16754054-16754073 MsG0280007535.01.T01:intron 40.0%
! TTCAGACTTCTGAAGAGACT+GGG - Chr2:16752265-16752284 MsG0280007535.01.T01:intron 40.0%
!! AGCAGAAAACCCTTTTGAGA+CGG + Chr2:16751862-16751881 None:intergenic 40.0%
!! CATCTCTAGTTCTAACCACT+TGG + Chr2:16752225-16752244 None:intergenic 40.0%
!! GCAATGAAAGTGATGGACAA+AGG - Chr2:16752343-16752362 MsG0280007535.01.T01:intron 40.0%
!! TGATTTGCTAGAAAAGAGCC+GGG + Chr2:16753732-16753751 None:intergenic 40.0%
!! TGCAGTTGATTGGTGGATTA+TGG - Chr2:16753898-16753917 MsG0280007535.01.T01:CDS 40.0%
!! TGTTGTTAGCTTTGCAGCAA+GGG - Chr2:16754027-16754046 MsG0280007535.01.T01:intron 40.0%
!!! CCTTTTTTGAAGGCGTGAAT+TGG - Chr2:16754129-16754148 MsG0280007535.01.T01:CDS 40.0%
AAACTCCGTTTAAGGGATCC+GGG - Chr2:16753950-16753969 MsG0280007535.01.T01:CDS 45.0%
AAAGGAACCTCAGCACAGAT+TGG - Chr2:16754072-16754091 MsG0280007535.01.T01:intron 45.0%
AACCGAGCCACATGTTTAAG+TGG - Chr2:16753973-16753992 MsG0280007535.01.T01:CDS 45.0%
AAGCTAACAACAGGTGCTTC+TGG + Chr2:16754019-16754038 None:intergenic 45.0%
ACGGAGAGAAATTTCGTGCT+TGG - Chr2:16752467-16752486 MsG0280007535.01.T01:CDS 45.0%
ACTTGTCTTCGCAATACTGC+TGG + Chr2:16752039-16752058 None:intergenic 45.0%
AGACTGGGTTGTGGAGATAT+TGG - Chr2:16752280-16752299 MsG0280007535.01.T01:intron 45.0%
AGCAGTATTGCGAAGACAAG+TGG - Chr2:16752038-16752057 MsG0280007535.01.T01:intron 45.0%
ATACAATCCCGAGCATGTGT+AGG + Chr2:16753485-16753504 None:intergenic 45.0%
ATCGAAGGGAAAGGTCAAGT+CGG + Chr2:16753565-16753584 None:intergenic 45.0%
CACATTAACAACGGATGAGG+AGG + Chr2:16751916-16751935 None:intergenic 45.0%
CCTGGCACCTGAAATAATCA+AGG - Chr2:16753859-16753878 MsG0280007535.01.T01:CDS 45.0%
CGACGAGGATTTAACTAGGT+TGG + Chr2:16753599-16753618 None:intergenic 45.0%
CTCCTCATTCGAACCATACA+TGG + Chr2:16752827-16752846 None:intergenic 45.0%
CTTGCTGCAAAGCTAACAAC+AGG + Chr2:16754028-16754047 None:intergenic 45.0%
GAAGTTACTTCGTGCTCAGA+CGG - Chr2:16752384-16752403 MsG0280007535.01.T01:CDS 45.0%
GAGCCACATGTTTAAGTGGT+AGG - Chr2:16753977-16753996 MsG0280007535.01.T01:intron 45.0%
GGTAAGAGCAGTATTTGCAG+AGG - Chr2:16752101-16752120 MsG0280007535.01.T01:intron 45.0%
GTATGGTTCGAATGAGGAGA+GGG - Chr2:16752828-16752847 MsG0280007535.01.T01:intron 45.0%
GTCTCTTCAGAAGTCTGAAG+TGG + Chr2:16752264-16752283 None:intergenic 45.0%
TAACAGCACATCGAAGGGAA+AGG + Chr2:16753574-16753593 None:intergenic 45.0%
TACAAAGTTGGAGAAACGGG+TGG + Chr2:16752434-16752453 None:intergenic 45.0%
TACACCACCTGAGATTCCAA+AGG - Chr2:16754169-16754188 MsG0280007535.01.T01:CDS 45.0%
TGCAGCAAGGGATCTTATCA+GGG - Chr2:16754039-16754058 MsG0280007535.01.T01:intron 45.0%
TGGACTAACAGCACATCGAA+GGG + Chr2:16753579-16753598 None:intergenic 45.0%
TGTATGGTTCGAATGAGGAG+AGG - Chr2:16752827-16752846 MsG0280007535.01.T01:intron 45.0%
TGTTTCTCTGCCGAAGAGTA+TGG + Chr2:16752520-16752539 None:intergenic 45.0%
TTGCAGCAAGGGATCTTATC+AGG - Chr2:16754038-16754057 MsG0280007535.01.T01:intron 45.0%
TTGGACTAACAGCACATCGA+AGG + Chr2:16753580-16753599 None:intergenic 45.0%
! AGCGTTACTTCTGAGTTGAG+TGG - Chr2:16752304-16752323 MsG0280007535.01.T01:intron 45.0%
! ATGGAGTACCTACACATGCT+CGG - Chr2:16753474-16753493 MsG0280007535.01.T01:intron 45.0%
! CCTTGATTATTTCAGGTGCC+AGG + Chr2:16753862-16753881 None:intergenic 45.0%
! CTTCAGACTTCTGAAGAGAC+TGG - Chr2:16752264-16752283 MsG0280007535.01.T01:intron 45.0%
! TAGGGTGTTCCGTCTCAAAA+GGG - Chr2:16751850-16751869 MsG0280007535.01.T01:intron 45.0%
! TCCTACCTCATGTTTTCACC+CGG - Chr2:16753711-16753730 MsG0280007535.01.T01:intron 45.0%
! TTGCAGATTTTACGTGGCAG+AGG - Chr2:16753442-16753461 MsG0280007535.01.T01:intron 45.0%
! TTTTACGTGGCAGAGGTTAC+TGG - Chr2:16753449-16753468 MsG0280007535.01.T01:intron 45.0%
!! CGAGCTGATTTTGTTGAGAG+TGG - Chr2:16752080-16752099 MsG0280007535.01.T01:intron 45.0%
!! CTGTTGTTAGCTTTGCAGCA+AGG - Chr2:16754026-16754045 MsG0280007535.01.T01:intron 45.0%
!! TCAACAGCCTTTGGAATCTC+AGG + Chr2:16754179-16754198 None:intergenic 45.0%
AACACAGGATGGCTGAATGC+AGG + Chr2:16753689-16753708 None:intergenic 50.0%
AACGCAAGGCCAAAACGAAC+TGG - Chr2:16753759-16753778 MsG0280007535.01.T01:intron 50.0%
AAGAGCCGGGTGAAAACATG+AGG + Chr2:16753719-16753738 None:intergenic 50.0%
ACATACTTCACTGCCTGCTC+AGG + Chr2:16752559-16752578 None:intergenic 50.0%
ACATGAGGTAGGAGCAACAC+AGG + Chr2:16753704-16753723 None:intergenic 50.0%
CATCCGGGATTCAAACACTG+AGG - Chr2:16751705-16751724 MsG0280007535.01.T01:CDS 50.0%
CTTCGGCAGAGAAACAACCA+GGG - Chr2:16752524-16752543 MsG0280007535.01.T01:intron 50.0%
GAGATGGGAAGCTATCCAAG+TGG - Chr2:16752207-16752226 MsG0280007535.01.T01:intron 50.0%
GCAGCAAGGGATCTTATCAG+GGG - Chr2:16754040-16754059 MsG0280007535.01.T01:intron 50.0%
GCGACAGCGAAGAAAACTTG+AGG + Chr2:16751738-16751757 None:intergenic 50.0%
GTGGAGACCTCCATACTCTT+CGG - Chr2:16752507-16752526 MsG0280007535.01.T01:intron 50.0%
GTTTCTGCGAAAGCTAGCGA+TGG - Chr2:16752011-16752030 MsG0280007535.01.T01:intron 50.0%
TACTTCGTGCTCAGACGGAA+CGG - Chr2:16752389-16752408 MsG0280007535.01.T01:CDS 50.0%
TCGCCTCAGTGTTTGAATCC+CGG + Chr2:16751711-16751730 None:intergenic 50.0%
TCTCAGGTGGTGTAGCACAT+CGG + Chr2:16754163-16754182 None:intergenic 50.0%
TCTTCGGCAGAGAAACAACC+AGG - Chr2:16752523-16752542 MsG0280007535.01.T01:intron 50.0%
TGCTCAGGAAAATGCTTCCC+TGG + Chr2:16752544-16752563 None:intergenic 50.0%
TGGTGTAGCACATCGGATCA+AGG + Chr2:16754156-16754175 None:intergenic 50.0%
TTAAGAGTAGGGTAGGGGAG+AGG - Chr2:16752878-16752897 MsG0280007535.01.T01:intron 50.0%
! CGCTGTCGCTTCAAAAGACA+GGG - Chr2:16751749-16751768 MsG0280007535.01.T01:CDS 50.0%
! CTTCTGAAGAGACTGGGTTG+TGG - Chr2:16752271-16752290 MsG0280007535.01.T01:intron 50.0%
! GTAGGGTGTTCCGTCTCAAA+AGG - Chr2:16751849-16751868 MsG0280007535.01.T01:intron 50.0%
! TCGCTGTCGCTTCAAAAGAC+AGG - Chr2:16751748-16751767 MsG0280007535.01.T01:CDS 50.0%
! TGGAGTACCTACACATGCTC+GGG - Chr2:16753475-16753494 MsG0280007535.01.T01:intron 50.0%
! TTCTCTGCCGAAGAGTATGG+AGG + Chr2:16752517-16752536 None:intergenic 50.0%
!! ACAGCCTTTGGAATCTCAGG+TGG + Chr2:16754176-16754195 None:intergenic 50.0%
!! AGGCTCTGAATCAATCGACG+AGG + Chr2:16753614-16753633 None:intergenic 50.0%
!! CTTGGTGATGGAATTCTGCC+GGG - Chr2:16752485-16752504 MsG0280007535.01.T01:intron 50.0%
!! GCTTGGTGATGGAATTCTGC+CGG - Chr2:16752484-16752503 MsG0280007535.01.T01:intron 50.0%
!! TAAACATGTGGCTCGGTTCC+CGG + Chr2:16753971-16753990 None:intergenic 50.0%
!!! AGGGAAGCATTTTCCTGAGC+AGG - Chr2:16752543-16752562 MsG0280007535.01.T01:intron 50.0%
ACGGCAGTGCAGTTGATTGG+TGG - Chr2:16753891-16753910 MsG0280007535.01.T01:CDS 55.0%
ACTTCGTGCTCAGACGGAAC+GGG - Chr2:16752390-16752409 MsG0280007535.01.T01:CDS 55.0%
AGAGTATGGAGGTCTCCACC+CGG + Chr2:16752506-16752525 None:intergenic 55.0%
CAACGACATAGAGCGAGCGT+CGG + Chr2:16753824-16753843 None:intergenic 55.0%
CAGGATGGCTGAATGCAGGA+TGG + Chr2:16753685-16753704 None:intergenic 55.0%
CGCTCGCTCTATGTCGTTGT+TGG - Chr2:16753825-16753844 MsG0280007535.01.T01:intron 55.0%
CGGTTCCCGGATCCCTTAAA+CGG + Chr2:16753958-16753977 None:intergenic 55.0%
GAGGTAGGAGCAACACAGGA+TGG + Chr2:16753700-16753719 None:intergenic 55.0%
GATGGCTGAATGCAGGATGG+CGG + Chr2:16753682-16753701 None:intergenic 55.0%
GCCGGGTGAAAACATGAGGT+AGG + Chr2:16753715-16753734 None:intergenic 55.0%
GGGGAGAGGAGGGATTCAAT+TGG - Chr2:16752892-16752911 MsG0280007535.01.T01:intron 55.0%
GTCACGGCAGTGCAGTTGAT+TGG - Chr2:16753888-16753907 MsG0280007535.01.T01:CDS 55.0%
GTGGCAGAGGTTACTGGCAA+TGG - Chr2:16753455-16753474 MsG0280007535.01.T01:intron 55.0%
TGCATCGTCTGCTGGCGATA+AGG - Chr2:16754223-16754242 MsG0280007535.01.T01:CDS 55.0%
! GATGGCGGCTCAATACATGC+AGG + Chr2:16753667-16753686 None:intergenic 55.0%
! GGTGCTTCTGGAAATCGCAG+CGG + Chr2:16754007-16754026 None:intergenic 55.0%
AGAGTAGGGTAGGGGAGAGG+AGG - Chr2:16752881-16752900 MsG0280007535.01.T01:intron 60.0%
GAGTAGGGTAGGGGAGAGGA+GGG - Chr2:16752882-16752901 MsG0280007535.01.T01:intron 60.0%
! GGTGGCTCTGCAATGAGCTC+AGG + Chr2:16753803-16753822 None:intergenic 60.0%
!! GGTGATGGAATTCTGCCGGG+TGG - Chr2:16752488-16752507 MsG0280007535.01.T01:intron 60.0%
CGCCAGCAGACGATGCAGCT+GGG + Chr2:16754220-16754239 None:intergenic 65.0%
GCAGACGATGCAGCTGGGGA+AGG + Chr2:16754215-16754234 None:intergenic 65.0%
GCCAGCAGACGATGCAGCTG+GGG + Chr2:16754219-16754238 None:intergenic 65.0%
TCCCCAGCTGCATCGTCTGC+TGG - Chr2:16754215-16754234 MsG0280007535.01.T01:CDS 65.0%
TCGCCAGCAGACGATGCAGC+TGG + Chr2:16754221-16754240 None:intergenic 65.0%
!! CGACATAGAGCGAGCGTCGG+TGG + Chr2:16753821-16753840 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 16751655 16754283 16751655 ID=MsG0280007535.01;Name=MsG0280007535.01
Chr2 mRNA 16751655 16754283 16751655 ID=MsG0280007535.01.T01;Parent=MsG0280007535.01;Name=MsG0280007535.01.T01;_AED=0.50;_eAED=0.54;_QI=0|0|0|1|1|1|4|0|234
Chr2 exon 16754090 16754283 16754090 ID=MsG0280007535.01.T01:exon:4415;Parent=MsG0280007535.01.T01
Chr2 exon 16753837 16753994 16753837 ID=MsG0280007535.01.T01:exon:4414;Parent=MsG0280007535.01.T01
Chr2 exon 16752349 16752490 16752349 ID=MsG0280007535.01.T01:exon:4413;Parent=MsG0280007535.01.T01
Chr2 exon 16751655 16751862 16751655 ID=MsG0280007535.01.T01:exon:4412;Parent=MsG0280007535.01.T01
Chr2 CDS 16754090 16754283 16754090 ID=MsG0280007535.01.T01:cds;Parent=MsG0280007535.01.T01
Chr2 CDS 16753837 16753994 16753837 ID=MsG0280007535.01.T01:cds;Parent=MsG0280007535.01.T01
Chr2 CDS 16752349 16752490 16752349 ID=MsG0280007535.01.T01:cds;Parent=MsG0280007535.01.T01
Chr2 CDS 16751655 16751862 16751655 ID=MsG0280007535.01.T01:cds;Parent=MsG0280007535.01.T01
Gene Sequence

>MsG0280007535.01.T01

ATGGACTCGAAAACTAGTGCTAGATCTCTATCTAAACAACATCAGAAAAACATCCGGGATTCAAACACTGAGGCGAAAGACCTCAAGTTTTCTTCGCTGTCGCTTCAAAAGACAGGGAAATCTGAGCCTGAAGATTTGTCTAACTCTGTACAAAACACGTCAAAGCAGAATATCTGCGAAAATATGAAAGTAAAGCTCACAAGAAAGCTCTATCGATCAAGACAAAAGACTTCAGAATATGGAAGTGTGAAAAACAGTTCTGTTTCTGCGAAAGCTAGCGATGGTGCCAGCAGTATTGCGAAGACAAGTGGAAGTGCTAAGATCAGTGACCGAGCTGATTTTGTTGAGAGTGATTTTACGTGGCAGAGGTTACTGGCAATGGAGTACCTACACATGCTCGGGATTGTATACCGTGATCTTAAGCCAGAAAATGTTCTTGTTAGGGATGATGGACATATTATGCTCTCCGACTTGACCTTTCCCTTCGATGTGCTGAACCTCAGCACAGATTGGCTTATAAAAGAGGCAACCGAGATTAAACAACATCCCTTTTTTGAAGGCGTGAATTGGGCCTTGATCCGATGTGCTACACCACCTGAGATTCCAAAGGCTGTTGAGTTTGAGAAGATACCTTCCCCAGCTGCATCGTCTGCTGGCGATAAGGCCGTTGTAACCACATGTCAACAACTCCTAATCAGAAAG
Protein sequence

>MsG0280007535.01.T01

MDSKTSARSLSKQHQKNIRDSNTEAKDLKFSSLSLQKTGKSEPEDLSNSVQNTSKQNICENMKVKLTRKLYRSRQKTSEYGSVKNSSVSAKASDGASSIAKTSGSAKISDRADFVESDFTWQRLLAMEYLHMLGIVYRDLKPENVLVRDDGHIMLSDLTFPFDVLNLSTDWLIKEATEIKQHPFFEGVNWALIRCATPPEIPKAVEFEKIPSPAASSAGDKAVVTTCQQLLIRK