AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007963.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007963.01.T01 MTR_2g436400 93.701 381 24 0 1 381 1 381 0.0 735
MsG0280007963.01.T01 MTR_2g437060 74.936 391 86 3 1 381 1 389 0.0 582
MsG0280007963.01.T01 MTR_2g437080 69.355 372 112 1 10 381 5 374 0.0 509
MsG0280007963.01.T01 MTR_2g437020 64.858 387 110 4 5 381 4 374 2.86e-173 488
MsG0280007963.01.T01 MTR_2g437030 66.406 384 111 3 10 381 83 460 1.22e-171 487
MsG0280007963.01.T01 MTR_2g436440 63.402 388 127 3 1 381 1 380 1.18e-169 479
MsG0280007963.01.T01 MTR_2g437040 63.533 351 128 0 31 381 24 374 2.38e-158 450
MsG0280007963.01.T01 MTR_4g119550 51.471 340 162 2 43 381 47 384 2.83e-122 359
MsG0280007963.01.T01 MTR_2g437170 49.606 381 173 5 20 381 14 394 1.61e-119 352
MsG0280007963.01.T01 MTR_2g437960 51.488 336 160 2 49 381 55 390 6.49e-118 348
MsG0280007963.01.T01 MTR_2g437150 49.468 376 171 5 20 376 14 389 1.26e-117 347
MsG0280007963.01.T01 MTR_2g437960 51.488 336 160 2 49 381 55 390 1.76e-117 348
MsG0280007963.01.T01 MTR_2g438010 50.575 348 161 3 43 381 62 407 3.67e-116 344
MsG0280007963.01.T01 MTR_2g437120 49.721 358 166 3 33 377 27 383 4.43e-115 342
MsG0280007963.01.T01 MTR_2g437160 49.171 362 171 3 33 381 38 399 3.11e-114 339
MsG0280007963.01.T01 MTR_2g437180 50.877 342 161 4 39 377 43 380 1.84e-113 337
MsG0280007963.01.T01 MTR_2g437100 50.543 368 159 6 33 377 28 395 5.36e-112 333
MsG0280007963.01.T01 MTR_2g437200 47.467 375 157 3 41 377 26 398 1.18e-111 332
MsG0280007963.01.T01 MTR_2g437130 47.425 369 168 5 24 381 22 375 2.77e-111 331
MsG0280007963.01.T01 MTR_2g076320 49.003 351 162 3 43 381 42 387 2.46e-110 329
MsG0280007963.01.T01 MTR_2g437990 48.406 345 172 2 43 381 42 386 3.57e-109 327
MsG0280007963.01.T01 MTR_2g437260 47.024 336 174 2 43 376 32 365 7.83e-108 322
MsG0280007963.01.T01 MTR_2g437240 44.974 378 168 3 42 381 27 402 4.34e-107 321
MsG0280007963.01.T01 MTR_2g436370 79.082 196 40 1 1 196 1 195 1.98e-106 311
MsG0280007963.01.T01 MTR_2g436460 45.906 342 182 2 37 377 37 376 1.78e-105 315
MsG0280007963.01.T01 MTR_2g438020 46.216 370 159 3 46 377 70 437 4.26e-103 313
MsG0280007963.01.T01 MTR_2g019840 45.932 381 187 7 10 381 5 375 1.01e-102 309
MsG0280007963.01.T01 MTR_2g437940 46.797 359 154 4 33 381 27 358 8.93e-101 303
MsG0280007963.01.T01 MTR_4g120380 42.896 366 203 3 12 375 9 370 2.16e-94 287
MsG0280007963.01.T01 MTR_2g019810 45.509 334 176 4 48 379 32 361 4.46e-94 286
MsG0280007963.01.T01 MTR_4g119580 42.566 343 195 1 33 375 30 370 2.12e-92 282
MsG0280007963.01.T01 MTR_4g120380 43.064 346 191 3 12 355 9 350 1.86e-88 273
MsG0280007963.01.T01 MTR_2g437090 69.136 162 50 0 220 381 1 162 7.24e-77 235
MsG0280007963.01.T01 MTR_2g437160 54.082 196 87 1 189 381 113 308 7.70e-66 212
MsG0280007963.01.T01 MTR_2g437160 41.379 116 53 3 33 137 38 149 8.67e-17 80.9
MsG0280007963.01.T01 MTR_3g450500 33.036 336 213 5 48 375 39 370 2.78e-54 184
MsG0280007963.01.T01 MTR_4g119570 38.057 247 120 2 127 373 14 227 7.36e-49 165
MsG0280007963.01.T01 MTR_4g007490 30.966 352 233 6 35 379 24 372 1.55e-48 168
MsG0280007963.01.T01 MTR_8g080990 28.814 295 207 2 80 371 74 368 2.01e-35 134
MsG0280007963.01.T01 MTR_8g081000 26.885 305 220 2 74 375 81 385 8.69e-33 127
MsG0280007963.01.T01 MTR_8g081000 27.619 315 205 6 74 375 81 385 1.00e-32 127
MsG0280007963.01.T01 MTR_8g081000 27.619 315 205 6 74 375 81 385 1.15e-32 127
MsG0280007963.01.T01 MTR_2g436380 55.752 113 43 3 220 332 1 106 1.72e-30 118
MsG0280007963.01.T01 MTR_4g049400 42.105 114 62 1 39 148 38 151 8.93e-21 90.5
MsG0280007963.01.T01 MTR_3g085240 62.264 53 20 0 279 331 176 228 1.94e-15 75.9
MsG0280007963.01.T01 MTR_3g085240 32.031 128 78 2 42 169 52 170 5.28e-12 65.9
MsG0280007963.01.T01 MTR_4g119615 60.000 50 20 0 319 368 26 75 8.45e-14 67.0
MsG0280007963.01.T01 MTR_2g019830 55.385 65 28 1 313 377 136 199 2.45e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007963.01.T01 AT5G07900 33.333 333 214 4 48 374 69 399 1.11e-62 206
MsG0280007963.01.T01 AT1G21150 28.857 350 237 6 37 378 9 354 4.17e-47 164
MsG0280007963.01.T01 AT1G21150 29.204 339 228 6 48 378 56 390 1.63e-45 161
MsG0280007963.01.T01 AT1G21150 29.204 339 228 6 48 378 120 454 6.67e-45 160
MsG0280007963.01.T01 AT1G61980 27.298 359 216 7 48 366 52 405 1.44e-36 137
MsG0280007963.01.T01 AT1G61980 27.298 359 216 7 48 366 52 405 1.44e-36 137
MsG0280007963.01.T01 AT1G61980 27.298 359 216 7 48 366 52 405 1.44e-36 137
MsG0280007963.01.T01 AT1G61970 26.857 350 211 7 51 360 55 399 1.56e-32 127
MsG0280007963.01.T01 AT1G61970 26.857 350 211 7 51 360 55 399 1.56e-32 127
MsG0280007963.01.T01 AT1G61970 26.857 350 211 7 51 360 55 399 1.56e-32 127
MsG0280007963.01.T01 AT1G61970 26.857 350 211 7 51 360 55 399 1.56e-32 127
MsG0280007963.01.T01 AT1G61970 26.857 350 211 7 51 360 55 399 1.56e-32 127
MsG0280007963.01.T01 AT1G61970 26.857 350 211 7 51 360 55 399 1.56e-32 127
MsG0280007963.01.T01 AT1G61970 26.857 350 211 7 51 360 55 399 1.56e-32 127
MsG0280007963.01.T01 AT5G64950 29.577 355 233 7 36 375 24 376 1.79e-32 126
MsG0280007963.01.T01 AT1G61990 27.717 368 208 9 51 374 55 408 1.18e-31 124
MsG0280007963.01.T01 AT1G61990 27.717 368 208 9 51 374 55 408 1.18e-31 124
MsG0280007963.01.T01 AT1G62120 26.536 358 216 8 51 366 59 411 6.22e-31 122
MsG0280007963.01.T01 AT1G62010 24.845 322 201 6 83 366 71 389 8.40e-27 110
MsG0280007963.01.T01 AT3G46950 28.295 258 166 4 42 292 46 291 8.42e-25 105
MsG0280007963.01.T01 AT1G62110 26.027 292 194 5 8 290 17 295 1.73e-24 104
MsG0280007963.01.T01 AT1G62085 26.515 396 209 14 48 366 55 445 1.42e-23 102
MsG0280007963.01.T01 AT1G62085 26.515 396 209 14 48 366 55 445 1.42e-23 102
MsG0280007963.01.T01 AT1G61960 28.571 231 161 3 51 279 55 283 1.83e-22 99.0
MsG0280007963.01.T01 AT5G23930 27.119 236 164 3 48 282 55 283 3.30e-19 89.4
MsG0280007963.01.T01 AT5G23930 20.904 354 236 12 50 365 89 436 2.43e-11 65.5
MsG0280007963.01.T01 AT1G62150 26.423 246 166 6 42 279 52 290 4.10e-18 86.3

Find 73 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATCTGACAAGGAAATTT+TGG 0.116874 2:+23779433 MsG0280007963.01.T01:CDS
TCCTACCTCATCAACAATTT+TGG 0.164455 2:+23779092 MsG0280007963.01.T01:CDS
GGAAAAGAGGGTTAGGGTTT+AGG 0.191466 2:-23779025 None:intergenic
TAAATTTAGTGATGAACTTT+TGG 0.199642 2:+23779804 MsG0280007963.01.T01:CDS
GTAAAGGAATTAAAGGATTT+GGG 0.236314 2:+23779608 MsG0280007963.01.T01:CDS
AGACCAACCCCATTTCTTAA+AGG 0.251614 2:-23779717 None:intergenic
GGTATTGTTTACTGTTGTTT+TGG 0.253046 2:-23779672 None:intergenic
ATGGTTGGTGGAACAGAGTT+TGG 0.256699 2:-23779135 None:intergenic
GTCACATTCTTTAGAAACTA+TGG 0.267215 2:+23779194 MsG0280007963.01.T01:CDS
GAAAAGAGGGTTAGGGTTTA+GGG 0.269920 2:-23779024 None:intergenic
GGTAAAGGAATTAAAGGATT+TGG 0.272443 2:+23779607 MsG0280007963.01.T01:CDS
AAACTTAATGACAAAATATA+AGG 0.279924 2:-23779869 None:intergenic
ATGGATGTTAACATAGAATA+AGG 0.283748 2:-23779773 None:intergenic
AAGAATGTGACAACAGATTC+AGG 0.288262 2:-23779182 None:intergenic
TGACAAAATATAAGGTTGTT+TGG 0.318444 2:-23779861 None:intergenic
AAACTTTGGCAACACCGTTT+TGG 0.327649 2:-23779282 None:intergenic
CAAGTCGGTAAAGGAATTAA+AGG 0.349745 2:+23779601 MsG0280007963.01.T01:CDS
TGTTCAACATCAATTCTTCA+TGG 0.364493 2:+23778940 MsG0280007963.01.T01:CDS
TTGGGTCAATCAGTTGGGTT+GGG 0.365374 2:+23779823 MsG0280007963.01.T01:CDS
AAGTTGATGCCTTTAAGAAA+TGG 0.366199 2:+23779708 MsG0280007963.01.T01:CDS
AGTTGATGCCTTTAAGAAAT+GGG 0.371481 2:+23779709 MsG0280007963.01.T01:CDS
GCCCTTGAGATAATAGATTA+GGG 0.372434 2:-23778976 None:intergenic
AAATGGTGGAAAAGAGGGTT+AGG 0.393469 2:-23779032 None:intergenic
AACTTATCAGTCACTACAAA+TGG 0.416436 2:-23779977 None:intergenic
GTCAGAGGTAGAAGCACCTT+TGG 0.417863 2:-23779318 None:intergenic
AGCCCTTGAGATAATAGATT+AGG 0.422295 2:-23778977 None:intergenic
CATCAACTTTCTCTTTCCAT+AGG 0.426803 2:-23779694 None:intergenic
TGGCAATCATATCGCGTAGT+TGG 0.438979 2:-23779229 None:intergenic
GGTTTGCAGGAAAGCAGCTC+TGG 0.441213 2:-23779257 None:intergenic
GGCAATCATATCGCGTAGTT+GGG 0.453430 2:-23779228 None:intergenic
CTGAAGTGTTGGTGCAGAAA+TGG 0.455082 2:-23779049 None:intergenic
AATAGTCCCAGCCTATGAAT+TGG 0.461317 2:+23779397 MsG0280007963.01.T01:CDS
GGAAAGCAGCTCTGGCATCT+TGG 0.471973 2:-23779249 None:intergenic
GAATCTGTACACCAATTCAT+AGG 0.473956 2:-23779408 None:intergenic
TTTGGGTCAATCAGTTGGGT+TGG 0.480856 2:+23779822 MsG0280007963.01.T01:CDS
CTGTACACCAATTCATAGGC+TGG 0.485721 2:-23779404 None:intergenic
ATCACAATGCTGATTCAGAA+TGG 0.490085 2:+23779509 MsG0280007963.01.T01:CDS
CTGGGACTATTTGATTCTTG+AGG 0.490835 2:-23779385 None:intergenic
CAGAGTTTGGAAGCAGATTG+TGG 0.492532 2:-23779122 None:intergenic
AACCCTAATCTATTATCTCA+AGG 0.495030 2:+23778974 MsG0280007963.01.T01:CDS
TCAGTTGGGTTGGGATGCTA+TGG 0.506950 2:+23779832 MsG0280007963.01.T01:CDS
AATATAAGGTTGTTTGGCAA+TGG 0.508469 2:-23779855 None:intergenic
AGAGTTTGGAAGCAGATTGT+GGG 0.513374 2:-23779121 None:intergenic
TGCAGAAATGGTGGAAAAGA+GGG 0.515244 2:-23779037 None:intergenic
ATCAACTTTCTCTTTCCATA+GGG 0.521600 2:-23779693 None:intergenic
TCACAATGCTGATTCAGAAT+GGG 0.524632 2:+23779510 MsG0280007963.01.T01:CDS
CAACAGTAAACAATACCCTA+TGG 0.530932 2:+23779678 MsG0280007963.01.T01:CDS
TGACATGCTCAAGTCGGTAA+AGG 0.531848 2:+23779592 MsG0280007963.01.T01:CDS
GTGCAGAAATGGTGGAAAAG+AGG 0.534389 2:-23779038 None:intergenic
GTGCAATTTCTTCTCAACAA+AGG 0.536369 2:+23779923 MsG0280007963.01.T01:CDS
TTCCTGCAAACCCTCCAAAA+CGG 0.545667 2:+23779268 MsG0280007963.01.T01:CDS
AATGGTGGAAAAGAGGGTTA+GGG 0.547641 2:-23779031 None:intergenic
CAAAACCAAAATTGTTGATG+AGG 0.555710 2:-23779097 None:intergenic
AATTGCACAACAGCGGCCCT+CGG 0.562081 2:-23779908 None:intergenic
AGGCTTACATACAAGACTCT+AGG 0.563542 2:-23779365 None:intergenic
TAAAGGAATTAAAGGATTTG+GGG 0.564846 2:+23779609 MsG0280007963.01.T01:CDS
TTTGAAACGAAGATGGTTGG+TGG 0.568434 2:-23779147 None:intergenic
CATTGCTGAGGGAACAGACT+CGG 0.569500 2:+23779555 MsG0280007963.01.T01:CDS
GAGGTAGGAGACTGCAAGAG+AGG 0.570893 2:-23779078 None:intergenic
GTTGATGCCTTTAAGAAATG+GGG 0.573670 2:+23779710 MsG0280007963.01.T01:CDS
CAGATTCTTGCAATCTGACA+AGG 0.575407 2:+23779424 MsG0280007963.01.T01:CDS
ACCAAAATTGTTGATGAGGT+AGG 0.577466 2:-23779093 None:intergenic
ACCCTAATCTATTATCTCAA+GGG 0.578641 2:+23778975 MsG0280007963.01.T01:CDS
AAACATTAAATCATTGCTGA+GGG 0.598387 2:+23779544 MsG0280007963.01.T01:CDS
ATGCCTTTAAGAAATGGGGT+TGG 0.598819 2:+23779714 MsG0280007963.01.T01:CDS
TGTACACCAATTCATAGGCT+GGG 0.603503 2:-23779403 None:intergenic
CAAACATTAAATCATTGCTG+AGG 0.611140 2:+23779543 MsG0280007963.01.T01:CDS
AGAGGTTGAATCTGAAGTGT+TGG 0.626308 2:-23779060 None:intergenic
GACAATGTTAACAATGTCAG+AGG 0.628038 2:-23779333 None:intergenic
AATAGATTAGGGTTCTTCTG+TGG 0.630114 2:-23778965 None:intergenic
GAGAAGAAATTGCACAACAG+CGG 0.635644 2:-23779915 None:intergenic
AAGTGTTGGTGCAGAAATGG+TGG 0.640434 2:-23779046 None:intergenic
GAGACGTGACATGCTCAAGT+CGG 0.669830 2:+23779586 MsG0280007963.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTTAATGACAAAATATA+AGG - Chr2:23779872-23779891 None:intergenic 15.0%
!!! CACTAAATTTATTTTATCAA+TGG - Chr2:23779795-23779814 None:intergenic 15.0%
!!! TTATATTTTGTCATTAAGTT+TGG + Chr2:23779871-23779890 MsG0280007963.01.T01:CDS 15.0%
!!! AAATTTAGTGATGAACTTTT+GGG + Chr2:23779805-23779824 MsG0280007963.01.T01:CDS 20.0%
!!! TAAATTTAGTGATGAACTTT+TGG + Chr2:23779804-23779823 MsG0280007963.01.T01:CDS 20.0%
!!! TTATCTTTTAAAGTTGTATG+AGG + Chr2:23780042-23780061 MsG0280007963.01.T01:CDS 20.0%
! AAACATTAAATCATTGCTGA+GGG + Chr2:23779544-23779563 MsG0280007963.01.T01:CDS 25.0%
! ATGGATGTTAACATAGAATA+AGG - Chr2:23779776-23779795 None:intergenic 25.0%
! GAGAGAAAAAATTGAAACTT+TGG - Chr2:23779299-23779318 None:intergenic 25.0%
! GTAAAGGAATTAAAGGATTT+GGG + Chr2:23779608-23779627 MsG0280007963.01.T01:CDS 25.0%
! TAAAGGAATTAAAGGATTTG+GGG + Chr2:23779609-23779628 MsG0280007963.01.T01:CDS 25.0%
! TGACAAAATATAAGGTTGTT+TGG - Chr2:23779864-23779883 None:intergenic 25.0%
! TGTCATTAAGTTTGGAAAAA+AGG + Chr2:23779879-23779898 MsG0280007963.01.T01:CDS 25.0%
!! AATCCTTCAAAAACAACTTT+TGG + Chr2:23779635-23779654 MsG0280007963.01.T01:CDS 25.0%
!!! TATAAAACGTTTTGAGAATG+AGG + Chr2:23780015-23780034 MsG0280007963.01.T01:CDS 25.0%
!!! TTTCAATTTTTTCTCTCCAA+AGG + Chr2:23779302-23779321 MsG0280007963.01.T01:CDS 25.0%
AACCCTAATCTATTATCTCA+AGG + Chr2:23778974-23778993 MsG0280007963.01.T01:CDS 30.0%
AACTTATCAGTCACTACAAA+TGG - Chr2:23779980-23779999 None:intergenic 30.0%
AATATAAGGTTGTTTGGCAA+TGG - Chr2:23779858-23779877 None:intergenic 30.0%
ACCCTAATCTATTATCTCAA+GGG + Chr2:23778975-23778994 MsG0280007963.01.T01:CDS 30.0%
ATCAACTTTCTCTTTCCATA+GGG - Chr2:23779696-23779715 None:intergenic 30.0%
CAAAACCAAAATTGTTGATG+AGG - Chr2:23779100-23779119 None:intergenic 30.0%
CAAACATTAAATCATTGCTG+AGG + Chr2:23779543-23779562 MsG0280007963.01.T01:CDS 30.0%
GGTAAAGGAATTAAAGGATT+TGG + Chr2:23779607-23779626 MsG0280007963.01.T01:CDS 30.0%
TGTTCAACATCAATTCTTCA+TGG + Chr2:23778940-23778959 MsG0280007963.01.T01:CDS 30.0%
! AGTTGATGCCTTTAAGAAAT+GGG + Chr2:23779709-23779728 MsG0280007963.01.T01:CDS 30.0%
! GTCACATTCTTTAGAAACTA+TGG + Chr2:23779194-23779213 MsG0280007963.01.T01:CDS 30.0%
! TTAGGGATTGTGATTTTTGT+GGG - Chr2:23779010-23779029 None:intergenic 30.0%
! TTTAGGGATTGTGATTTTTG+TGG - Chr2:23779011-23779030 None:intergenic 30.0%
!! AAGTTGATGCCTTTAAGAAA+TGG + Chr2:23779708-23779727 MsG0280007963.01.T01:CDS 30.0%
!! GGTATTGTTTACTGTTGTTT+TGG - Chr2:23779675-23779694 None:intergenic 30.0%
!!! ACACCAAAAGTTGTTTTTGA+AGG - Chr2:23779641-23779660 None:intergenic 30.0%
AAGAATGTGACAACAGATTC+AGG - Chr2:23779185-23779204 None:intergenic 35.0%
AATAGATTAGGGTTCTTCTG+TGG - Chr2:23778968-23778987 None:intergenic 35.0%
AGCCCTTGAGATAATAGATT+AGG - Chr2:23778980-23778999 None:intergenic 35.0%
CAACAGTAAACAATACCCTA+TGG + Chr2:23779678-23779697 MsG0280007963.01.T01:CDS 35.0%
CAAGTCGGTAAAGGAATTAA+AGG + Chr2:23779601-23779620 MsG0280007963.01.T01:CDS 35.0%
CATCAACTTTCTCTTTCCAT+AGG - Chr2:23779697-23779716 None:intergenic 35.0%
GAATCTGTACACCAATTCAT+AGG - Chr2:23779411-23779430 None:intergenic 35.0%
GACAATGTTAACAATGTCAG+AGG - Chr2:23779336-23779355 None:intergenic 35.0%
GCCCTTGAGATAATAGATTA+GGG - Chr2:23778979-23778998 None:intergenic 35.0%
GTGCAATTTCTTCTCAACAA+AGG + Chr2:23779923-23779942 MsG0280007963.01.T01:CDS 35.0%
TATGAGGAAAAACGCAATCT+AGG + Chr2:23780058-23780077 MsG0280007963.01.T01:CDS 35.0%
! ACCAAAATTGTTGATGAGGT+AGG - Chr2:23779096-23779115 None:intergenic 35.0%
! GCAATCTGACAAGGAAATTT+TGG + Chr2:23779433-23779452 MsG0280007963.01.T01:CDS 35.0%
! GTTGATGCCTTTAAGAAATG+GGG + Chr2:23779710-23779729 MsG0280007963.01.T01:CDS 35.0%
! TAGGGATTGTGATTTTTGTG+GGG - Chr2:23779009-23779028 None:intergenic 35.0%
! TCCTACCTCATCAACAATTT+TGG + Chr2:23779092-23779111 MsG0280007963.01.T01:CDS 35.0%
!! AACTTTTGGGTCAATCAGTT+GGG + Chr2:23779818-23779837 MsG0280007963.01.T01:CDS 35.0%
!! ATCACAATGCTGATTCAGAA+TGG + Chr2:23779509-23779528 MsG0280007963.01.T01:CDS 35.0%
!! GACAACAGATTCAGGTTTTT+GGG - Chr2:23779177-23779196 None:intergenic 35.0%
!! TCACAATGCTGATTCAGAAT+GGG + Chr2:23779510-23779529 MsG0280007963.01.T01:CDS 35.0%
!! TGACAACAGATTCAGGTTTT+TGG - Chr2:23779178-23779197 None:intergenic 35.0%
AATAGTCCCAGCCTATGAAT+TGG + Chr2:23779397-23779416 MsG0280007963.01.T01:CDS 40.0%
AGACCAACCCCATTTCTTAA+AGG - Chr2:23779720-23779739 None:intergenic 40.0%
AGAGTTTGGAAGCAGATTGT+GGG - Chr2:23779124-23779143 None:intergenic 40.0%
AGGCTTACATACAAGACTCT+AGG - Chr2:23779368-23779387 None:intergenic 40.0%
CAGATTCTTGCAATCTGACA+AGG + Chr2:23779424-23779443 MsG0280007963.01.T01:CDS 40.0%
CTGGGACTATTTGATTCTTG+AGG - Chr2:23779388-23779407 None:intergenic 40.0%
GAAAAGAGGGTTAGGGTTTA+GGG - Chr2:23779027-23779046 None:intergenic 40.0%
GAGAAGAAATTGCACAACAG+CGG - Chr2:23779918-23779937 None:intergenic 40.0%
GGAAAAAAGGATCATTCCGA+GGG + Chr2:23779892-23779911 MsG0280007963.01.T01:CDS 40.0%
TGGAAAAAAGGATCATTCCG+AGG + Chr2:23779891-23779910 MsG0280007963.01.T01:CDS 40.0%
TGTACACCAATTCATAGGCT+GGG - Chr2:23779406-23779425 None:intergenic 40.0%
! AAACTTTGGCAACACCGTTT+TGG - Chr2:23779285-23779304 None:intergenic 40.0%
! AAATGGTGGAAAAGAGGGTT+AGG - Chr2:23779035-23779054 None:intergenic 40.0%
! AATGGTGGAAAAGAGGGTTA+GGG - Chr2:23779034-23779053 None:intergenic 40.0%
! AGAGGTTGAATCTGAAGTGT+TGG - Chr2:23779063-23779082 None:intergenic 40.0%
! ATGCCTTTAAGAAATGGGGT+TGG + Chr2:23779714-23779733 MsG0280007963.01.T01:CDS 40.0%
! TGCAGAAATGGTGGAAAAGA+GGG - Chr2:23779040-23779059 None:intergenic 40.0%
! TTTGAAACGAAGATGGTTGG+TGG - Chr2:23779150-23779169 None:intergenic 40.0%
!! AACAGATTCAGGTTTTTGGG+TGG - Chr2:23779174-23779193 None:intergenic 40.0%
!! GAACTTTTGGGTCAATCAGT+TGG + Chr2:23779817-23779836 MsG0280007963.01.T01:CDS 40.0%
!!! GGTTTTGAAACGAAGATGGT+TGG - Chr2:23779153-23779172 None:intergenic 40.0%
CAGAGTTTGGAAGCAGATTG+TGG - Chr2:23779125-23779144 None:intergenic 45.0%
CTGTACACCAATTCATAGGC+TGG - Chr2:23779407-23779426 None:intergenic 45.0%
GGAAAAGAGGGTTAGGGTTT+AGG - Chr2:23779028-23779047 None:intergenic 45.0%
GGCAATCATATCGCGTAGTT+GGG - Chr2:23779231-23779250 None:intergenic 45.0%
TGACATGCTCAAGTCGGTAA+AGG + Chr2:23779592-23779611 MsG0280007963.01.T01:CDS 45.0%
TGGCAATCATATCGCGTAGT+TGG - Chr2:23779232-23779251 None:intergenic 45.0%
TTCCTGCAAACCCTCCAAAA+CGG + Chr2:23779268-23779287 MsG0280007963.01.T01:CDS 45.0%
TTGGGTCAATCAGTTGGGTT+GGG + Chr2:23779823-23779842 MsG0280007963.01.T01:CDS 45.0%
TTTGGGTCAATCAGTTGGGT+TGG + Chr2:23779822-23779841 MsG0280007963.01.T01:CDS 45.0%
! GTGCAGAAATGGTGGAAAAG+AGG - Chr2:23779041-23779060 None:intergenic 45.0%
!! AAGTGTTGGTGCAGAAATGG+TGG - Chr2:23779049-23779068 None:intergenic 45.0%
!! ATGGTTGGTGGAACAGAGTT+TGG - Chr2:23779138-23779157 None:intergenic 45.0%
!! CTGAAGTGTTGGTGCAGAAA+TGG - Chr2:23779052-23779071 None:intergenic 45.0%
!! TTTGGCAACACCGTTTTGGA+GGG - Chr2:23779281-23779300 None:intergenic 45.0%
!!! GGGTGGTTTTGAAACGAAGA+TGG - Chr2:23779157-23779176 None:intergenic 45.0%
CATTGCTGAGGGAACAGACT+CGG + Chr2:23779555-23779574 MsG0280007963.01.T01:CDS 50.0%
GAGACGTGACATGCTCAAGT+CGG + Chr2:23779586-23779605 MsG0280007963.01.T01:CDS 50.0%
! CTTTGGCAACACCGTTTTGG+AGG - Chr2:23779282-23779301 None:intergenic 50.0%
!! GTCAGAGGTAGAAGCACCTT+TGG - Chr2:23779321-23779340 None:intergenic 50.0%
!! TCAGTTGGGTTGGGATGCTA+TGG + Chr2:23779832-23779851 MsG0280007963.01.T01:CDS 50.0%
AATTGCACAACAGCGGCCCT+CGG - Chr2:23779911-23779930 None:intergenic 55.0%
GAGGTAGGAGACTGCAAGAG+AGG - Chr2:23779081-23779100 None:intergenic 55.0%
GGTTTGCAGGAAAGCAGCTC+TGG - Chr2:23779260-23779279 None:intergenic 55.0%
! GGAAAGCAGCTCTGGCATCT+TGG - Chr2:23779252-23779271 None:intergenic 55.0%
!!! CACCGTTTTGGAGGGTTTGC+AGG - Chr2:23779273-23779292 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 23778939 23780084 23778939 ID=MsG0280007963.01;Name=MsG0280007963.01
Chr2 mRNA 23778939 23780084 23778939 ID=MsG0280007963.01.T01;Parent=MsG0280007963.01;Name=MsG0280007963.01.T01;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|381
Chr2 exon 23778939 23780084 23778939 ID=MsG0280007963.01.T01:exon:3027;Parent=MsG0280007963.01.T01
Chr2 CDS 23778939 23780084 23778939 ID=MsG0280007963.01.T01:cds;Parent=MsG0280007963.01.T01
Gene Sequence

>MsG0280007963.01.T01

ATGTTCAACATCAATTCTTCATGGCGCCACAGAAGAACCCTAATCTATTATCTCAAGGGCTTCGCTACCCCACAAAAATCACAATCCCTAAACCCTAACCCTCTTTTCCACCATTTCTGCACCAACACTTCAGATTCAACCTCTCTTGCAGTCTCCTACCTCATCAACAATTTTGGTTTTGACCCACAATCTGCTTCCAAACTCTGTTCCACCAACCATCTTCGTTTCAAAACCACCCAAAAACCTGAATCTGTTGTCACATTCTTTAGAAACTATGGTTTCTCTAATTCCCAACTACGCGATATGATTGCCAAGATGCCAGAGCTGCTTTCCTGCAAACCCTCCAAAACGGTGTTGCCAAAGTTTCAATTTTTTCTCTCCAAAGGTGCTTCTACCTCTGACATTGTTAACATTGTCAGTAAGACTCCTAGAGTCTTGTATGTAAGCCTCAAGAATCAAATAGTCCCAGCCTATGAATTGGTGTACAGATTCTTGCAATCTGACAAGGAAATTTTGGAAAAGTTGAATTACAGTGCAAATTTACTTTGTGATTGTACTGTGCAAAATAACATCACAATGCTGATTCAGAATGGGATGTCTGACTCAAACATTAAATCATTGCTGAGGGAACAGACTCGGATATTCAAGAGACGTGACATGCTCAAGTCGGTAAAGGAATTAAAGGATTTGGGGTTTAATCCTTCAAAAACAACTTTTGGTGTAGCATTGCATGCCAAAACAACAGTAAACAATACCCTATGGAAAGAGAAAGTTGATGCCTTTAAGAAATGGGGTTGGTCTGAAGAAGATTCTCTCGAAGCATTTAGAAAGAAACCTTATTCTATGTTAACATCCATTGATAAAATAAATTTAGTGATGAACTTTTGGGTCAATCAGTTGGGTTGGGATGCTATGGCCATTGCCAAACAACCTTATATTTTGTCATTAAGTTTGGAAAAAAGGATCATTCCGAGGGCCGCTGTTGTGCAATTTCTTCTCAACAAAGGTTTGACAGATAAGAACGCAAGCTTAACTTATCCATTTGTAGTGACTGATAAGTTGTTTCTTAATATGTTTATAAAACGTTTTGAGAATGAGGCTTCTTATCTTTTAAAGTTGTATGAGGAAAAACGCAATCTAGGATAG
Protein sequence

>MsG0280007963.01.T01

MFNINSSWRHRRTLIYYLKGFATPQKSQSLNPNPLFHHFCTNTSDSTSLAVSYLINNFGFDPQSASKLCSTNHLRFKTTQKPESVVTFFRNYGFSNSQLRDMIAKMPELLSCKPSKTVLPKFQFFLSKGASTSDIVNIVSKTPRVLYVSLKNQIVPAYELVYRFLQSDKEILEKLNYSANLLCDCTVQNNITMLIQNGMSDSNIKSLLREQTRIFKRRDMLKSVKELKDLGFNPSKTTFGVALHAKTTVNNTLWKEKVDAFKKWGWSEEDSLEAFRKKPYSMLTSIDKINLVMNFWVNQLGWDAMAIAKQPYILSLSLEKRIIPRAAVVQFLLNKGLTDKNASLTYPFVVTDKLFLNMFIKRFENEASYLLKLYEEKRNLG*