AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280008016.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008016.01.T01 MTR_2g437170 77.296 392 82 3 69 455 23 412 0.0 604
MsG0280008016.01.T01 MTR_2g437150 73.890 383 93 3 69 446 23 403 0.0 560
MsG0280008016.01.T01 MTR_2g437160 69.324 414 117 3 43 449 1 411 0.0 553
MsG0280008016.01.T01 MTR_2g437960 69.343 411 112 4 54 455 1 406 0.0 532
MsG0280008016.01.T01 MTR_2g437960 69.363 408 111 4 54 452 1 403 0.0 526
MsG0280008016.01.T01 MTR_2g437100 69.309 391 111 4 52 433 5 395 0.0 517
MsG0280008016.01.T01 MTR_2g437120 66.915 402 116 4 54 445 1 395 1.03e-178 506
MsG0280008016.01.T01 MTR_2g437180 66.005 403 117 4 54 446 1 393 8.21e-178 503
MsG0280008016.01.T01 MTR_2g438010 65.608 378 126 2 76 453 48 421 1.60e-172 491
MsG0280008016.01.T01 MTR_2g437990 66.578 377 121 3 76 452 28 399 1.41e-167 478
MsG0280008016.01.T01 MTR_2g076320 66.138 378 124 2 76 453 28 401 1.40e-166 475
MsG0280008016.01.T01 MTR_2g437200 65.195 385 93 3 84 433 20 398 1.08e-161 462
MsG0280008016.01.T01 MTR_2g437940 61.707 410 109 5 54 453 1 372 2.24e-160 458
MsG0280008016.01.T01 MTR_2g437240 61.314 411 112 5 78 453 18 416 2.95e-159 457
MsG0280008016.01.T01 MTR_2g437130 60.309 388 134 6 67 446 9 384 4.73e-149 430
MsG0280008016.01.T01 MTR_2g438020 58.612 389 121 3 99 452 70 453 6.50e-145 423
MsG0280008016.01.T01 MTR_2g437020 52.141 397 162 8 54 441 1 378 1.42e-121 360
MsG0280008016.01.T01 MTR_2g437080 51.124 356 163 2 94 445 34 382 1.26e-119 355
MsG0280008016.01.T01 MTR_2g436440 50.129 387 172 5 65 445 17 388 4.54e-118 351
MsG0280008016.01.T01 MTR_2g437060 51.226 367 169 3 75 441 37 393 5.35e-118 351
MsG0280008016.01.T01 MTR_2g437030 51.705 352 167 1 94 445 120 468 3.73e-115 346
MsG0280008016.01.T01 MTR_2g437040 50.420 357 166 3 93 445 33 382 9.43e-112 335
MsG0280008016.01.T01 MTR_2g436400 49.709 344 170 1 94 437 41 381 4.67e-110 330
MsG0280008016.01.T01 MTR_2g437260 46.240 359 182 4 84 440 24 373 7.39e-105 317
MsG0280008016.01.T01 MTR_4g119550 46.839 348 179 3 95 441 46 388 4.93e-102 310
MsG0280008016.01.T01 MTR_4g120380 46.628 341 177 2 90 430 34 369 1.26e-97 298
MsG0280008016.01.T01 MTR_2g436460 47.522 343 174 4 93 434 40 377 5.42e-93 286
MsG0280008016.01.T01 MTR_2g437160 74.194 186 48 0 264 449 135 320 1.66e-92 283
MsG0280008016.01.T01 MTR_2g437160 56.051 157 56 3 43 192 1 151 2.09e-42 152
MsG0280008016.01.T01 MTR_4g119580 43.396 371 201 4 64 430 4 369 3.87e-92 284
MsG0280008016.01.T01 MTR_4g120380 47.205 322 165 2 90 411 34 350 6.85e-92 285
MsG0280008016.01.T01 MTR_2g019840 40.431 371 199 8 77 433 9 371 1.17e-74 239
MsG0280008016.01.T01 MTR_2g019810 42.105 342 177 7 100 433 31 359 8.26e-73 234
MsG0280008016.01.T01 MTR_2g437090 50.877 171 84 0 276 446 1 171 2.02e-56 184
MsG0280008016.01.T01 MTR_4g119570 38.057 247 117 3 183 429 17 227 5.35e-48 165
MsG0280008016.01.T01 MTR_3g450500 28.767 365 252 4 69 430 10 369 8.33e-46 163
MsG0280008016.01.T01 MTR_4g007490 30.145 345 235 4 89 430 26 367 2.14e-42 154
MsG0280008016.01.T01 MTR_2g436370 45.860 157 82 2 94 250 40 193 2.28e-36 133
MsG0280008016.01.T01 MTR_4g049400 48.026 152 72 3 54 200 1 150 5.97e-36 133
MsG0280008016.01.T01 MTR_8g081000 28.000 300 212 2 133 430 87 384 1.24e-27 114
MsG0280008016.01.T01 MTR_8g081000 28.000 300 212 2 133 430 87 384 1.67e-27 114
MsG0280008016.01.T01 MTR_8g081000 28.000 300 212 2 133 430 87 384 1.67e-27 114
MsG0280008016.01.T01 MTR_8g080990 28.620 297 208 2 133 427 74 368 3.44e-26 109
MsG0280008016.01.T01 MTR_3g085240 56.522 92 23 1 333 424 174 248 1.12e-22 97.4
MsG0280008016.01.T01 MTR_3g085240 33.714 175 85 6 84 255 48 194 1.19e-15 77.0
MsG0280008016.01.T01 MTR_2g436380 58.333 60 25 0 276 335 1 60 2.34e-15 76.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008016.01.T01 AT5G07900 33.244 373 231 7 70 430 33 399 1.73e-55 190
MsG0280008016.01.T01 AT1G21150 30.000 330 226 3 102 428 57 384 9.96e-43 155
MsG0280008016.01.T01 AT1G21150 29.641 334 230 3 98 428 17 348 1.34e-42 154
MsG0280008016.01.T01 AT1G21150 30.000 330 226 3 102 428 121 448 8.31e-42 154
MsG0280008016.01.T01 AT5G64950 29.107 347 235 7 93 430 31 375 2.96e-30 121
MsG0280008016.01.T01 AT1G61980 28.291 357 204 9 102 416 53 399 2.50e-25 107
MsG0280008016.01.T01 AT1G61980 28.291 357 204 9 102 416 53 399 2.50e-25 107
MsG0280008016.01.T01 AT1G61980 28.291 357 204 9 102 416 53 399 2.50e-25 107
MsG0280008016.01.T01 AT1G61990 25.722 381 233 6 56 400 9 375 7.20e-25 106
MsG0280008016.01.T01 AT1G61990 25.722 381 233 6 56 400 9 375 7.20e-25 106
MsG0280008016.01.T01 AT3G46950 28.296 311 195 8 79 378 31 324 3.95e-24 104
MsG0280008016.01.T01 AT1G61970 26.479 355 213 8 102 416 53 399 2.70e-23 102
MsG0280008016.01.T01 AT1G61970 26.479 355 213 8 102 416 53 399 2.70e-23 102
MsG0280008016.01.T01 AT1G61970 26.479 355 213 8 102 416 53 399 2.70e-23 102
MsG0280008016.01.T01 AT1G61970 26.479 355 213 8 102 416 53 399 2.70e-23 102
MsG0280008016.01.T01 AT1G61970 26.479 355 213 8 102 416 53 399 2.70e-23 102
MsG0280008016.01.T01 AT1G61970 26.479 355 213 8 102 416 53 399 2.70e-23 102
MsG0280008016.01.T01 AT1G61970 26.479 355 213 8 102 416 53 399 2.70e-23 102
MsG0280008016.01.T01 AT1G62120 26.549 339 210 5 91 393 46 381 3.69e-23 101
MsG0280008016.01.T01 AT1G62110 27.799 259 183 3 89 346 40 295 1.85e-21 97.1
MsG0280008016.01.T01 AT1G61960 28.425 292 202 5 56 346 9 294 1.95e-21 97.1
MsG0280008016.01.T01 AT1G62085 26.398 322 213 9 75 378 18 333 8.82e-20 92.0
MsG0280008016.01.T01 AT1G62085 26.398 322 213 9 75 378 18 333 8.82e-20 92.0
MsG0280008016.01.T01 AT1G62150 27.755 245 173 3 102 345 59 300 2.59e-17 84.3
MsG0280008016.01.T01 AT5G23930 26.316 304 200 7 87 378 35 326 4.94e-16 80.5
MsG0280008016.01.T01 AT1G62010 25.170 294 179 4 136 393 71 359 3.51e-14 74.7

Find 79 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGAACCATTTCGTTATTC+TGG 0.141446 2:+24337574 MsG0280008016.01.T01:CDS
ATCAATTTAATGTCTGATTT+TGG 0.143567 2:+24337112 MsG0280008016.01.T01:CDS
TCATACCTTATCAACAATTT+TGG 0.165317 2:+24336686 MsG0280008016.01.T01:CDS
AAGATGGTGATGGCAAATTT+TGG 0.202387 2:+24334983 None:intergenic
TTCAATTGATAAGATTAATT+TGG 0.211307 2:+24337393 MsG0280008016.01.T01:CDS
AAGCTACTTCGTAAAATTCT+AGG 0.214273 2:-24336959 None:intergenic
TGGAGGAAGTTAAGAGTTTA+GGG 0.232380 2:+24337212 MsG0280008016.01.T01:CDS
TTGGAGGAAGTTAAGAGTTT+AGG 0.244148 2:+24337211 MsG0280008016.01.T01:CDS
TGAAAATTTACTCGAATGAT+AGG 0.246857 2:-24336506 None:intergenic
TATATTAGACTTCATCAAAA+TGG 0.287812 2:+24335093 MsG0280008016.01.T01:CDS
ATTCCTTGGCACTTGCTAAA+AGG 0.292312 2:+24337449 MsG0280008016.01.T01:CDS
CATCCTTGGACGAATACTTT+TGG 0.303240 2:+24337238 MsG0280008016.01.T01:CDS
AAGAAGGTGAGAACTGAATT+AGG 0.306086 2:-24336776 None:intergenic
AATTGATATTAGACACAATA+AGG 0.329025 2:-24337096 None:intergenic
TAGACCAACCCCATTTCTTA+AGG 0.338165 2:-24337321 None:intergenic
AAAGGATTTGATTAACTCAT+TGG 0.346872 2:+24337192 MsG0280008016.01.T01:CDS
CAGTCAAGGCCATCTACATT+TGG 0.353370 2:+24337166 MsG0280008016.01.T01:CDS
CAAGTTACACATTCGGTAGA+TGG 0.370763 2:-24337715 None:intergenic
AAGTTGATGCCCTTAAGAAA+TGG 0.388575 2:+24337311 MsG0280008016.01.T01:CDS
TAGTATCTGATGAGTGAAAT+CGG 0.388813 2:-24336611 None:intergenic
AGAGTTTAGGGTTTCATCCT+TGG 0.397034 2:+24337224 MsG0280008016.01.T01:CDS
AGTTGATGCCCTTAAGAAAT+GGG 0.397811 2:+24337312 MsG0280008016.01.T01:CDS
TTGAACCATTTCGTTATTCT+GGG 0.398296 2:+24337575 MsG0280008016.01.T01:CDS
TCCTTTGAACCAAATGTAGA+TGG 0.407709 2:-24337175 None:intergenic
TCAAGGTGAAGGTTACTATC+AGG 0.421923 2:-24335045 None:intergenic
GCAAACAATGGTATCCTTGT+CGG 0.427133 2:-24337032 None:intergenic
AGAAACCAAAATTGTTGATA+AGG 0.433609 2:-24336691 None:intergenic
GCCATCTACATTTGGTTCAA+AGG 0.443477 2:+24337174 MsG0280008016.01.T01:CDS
AGACCAACCCCATTTCTTAA+GGG 0.447273 2:-24337320 None:intergenic
AACAATTGTTCCAAGGGCAT+TGG 0.458416 2:+24337501 MsG0280008016.01.T01:CDS
AGCTACTTCGTAAAATTCTA+GGG 0.465397 2:-24336958 None:intergenic
ACTATCAGGATAGGTGTCTC+TGG 0.469495 2:-24335031 None:intergenic
TCAATTGGGTGGGAATTCCT+TGG 0.472262 2:+24337435 MsG0280008016.01.T01:CDS
ATGAAGTCTAATATAGAGTC+TGG 0.476178 2:-24335085 None:intergenic
CTGCAATATCTTGTGATGAA+AGG 0.485086 2:+24337526 MsG0280008016.01.T01:CDS
ACCTAAAACTACTACATTTG+TGG 0.495767 2:-24336556 None:intergenic
ACATTCTAAAAGGACCTGTC+TGG 0.505449 2:+24335006 MsG0280008016.01.T01:CDS
ATCAGAGGAAGAAGCACCAT+TGG 0.505631 2:-24336912 None:intergenic
ATATAGAGTCTGGTGGGTAA+TGG 0.506889 2:-24335075 None:intergenic
ATCTTGTGATGAAAGGTTTG+AGG 0.509536 2:+24337533 MsG0280008016.01.T01:CDS
TGCAGAAAACAATTGTTCCA+AGG 0.522766 2:+24337494 MsG0280008016.01.T01:CDS
GAAAATTTACTCGAATGATA+GGG 0.523466 2:-24336505 None:intergenic
TGATTAACAAGTTACACATT+CGG 0.525291 2:-24337722 None:intergenic
CAAGCATTTAGGATGCATCC+TGG 0.527087 2:+24337358 MsG0280008016.01.T01:CDS
ACACATTCGGTAGATGGCAT+TGG 0.528449 2:-24337709 None:intergenic
TTGTTTGCTTAATTCGATGT+TGG 0.530080 2:+24337047 MsG0280008016.01.T01:CDS
ATTAGACACAATAAGGTGGT+AGG 0.531490 2:-24337089 None:intergenic
TTTCATCACAGCCCCACAAA+AGG 0.535209 2:+24336861 MsG0280008016.01.T01:CDS
GCAGAAAACAATTGTTCCAA+GGG 0.535861 2:+24337495 MsG0280008016.01.T01:CDS
CAAAGCTTTCAACAACAAGC+AGG 0.537532 2:+24336730 MsG0280008016.01.T01:CDS
AGTATCTGATGAGTGAAATC+GGG 0.544517 2:-24336610 None:intergenic
AGGTGTCTCTGGAACCAGAC+AGG 0.549310 2:-24335020 None:intergenic
AAAGCTTTGAGAGCAGATTG+AGG 0.549590 2:-24336716 None:intergenic
TATTGCAGAACCAATGCCCT+TGG 0.552410 2:-24337511 None:intergenic
TTTGGGTTAATCAATTGGGT+GGG 0.559000 2:+24337425 MsG0280008016.01.T01:CDS
TCGGGAACAACAAGTGTTGA+GGG 0.560234 2:-24336592 None:intergenic
ATCGGGAACAACAAGTGTTG+AGG 0.562201 2:-24336593 None:intergenic
GGATTTGATTAACTCATTGG+AGG 0.570633 2:+24337195 MsG0280008016.01.T01:CDS
GTGAAGGTTACTATCAGGAT+AGG 0.572776 2:-24335040 None:intergenic
CTCACCTTCTTAGAAAACCA+TGG 0.576168 2:+24336788 MsG0280008016.01.T01:CDS
TAAGGTATGATACAGCGAAT+GGG 0.582706 2:-24336673 None:intergenic
TTGCTAGCTCGCTTCAGTCA+AGG 0.593225 2:+24337152 MsG0280008016.01.T01:CDS
TTAGACACAATAAGGTGGTA+GGG 0.595223 2:-24337088 None:intergenic
AGTCTAATATAGAGTCTGGT+GGG 0.598953 2:-24335081 None:intergenic
ATAAGGTATGATACAGCGAA+TGG 0.604855 2:-24336674 None:intergenic
ATTCCAAAAGTATTCGTCCA+AGG 0.607263 2:-24337241 None:intergenic
AAGTCTAATATAGAGTCTGG+TGG 0.613144 2:-24335082 None:intergenic
GAGAAACAATTTCTTCAACA+TGG 0.614078 2:-24336531 None:intergenic
TAACGATGAAACAATATCAG+AGG 0.619380 2:-24336927 None:intergenic
ATGCCCTTAAGAAATGGGGT+TGG 0.630132 2:+24337317 MsG0280008016.01.T01:CDS
GTTGATGCCCTTAAGAAATG+GGG 0.641680 2:+24337313 MsG0280008016.01.T01:CDS
ATTGAAGTCAACATGAAACC+AGG 0.642061 2:-24337376 None:intergenic
ACATCGAATTAAGCAAACAA+TGG 0.642451 2:-24337044 None:intergenic
GGCTGTGATGAAAGTAACCA+AGG 0.658789 2:-24336851 None:intergenic
TAAAACTACTACATTTGTGG+CGG 0.666272 2:-24336553 None:intergenic
TAGGTTCTTTAAAACCGACA+AGG 0.672846 2:+24337018 MsG0280008016.01.T01:CDS
GCATACACAAAAGAGAACAA+TGG 0.682398 2:+24337682 MsG0280008016.01.T01:CDS
CTAGTGAATCAAGATGGTGA+TGG 0.682561 2:+24334973 None:intergenic
TGATATTAGACACAATAAGG+TGG 0.695337 2:-24337093 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATATATTTTTTTTTAAAA+AGG + Chr2:24336053-24336072 MsG0280008016.01.T01:intron 0.0%
!!! TATTATAAATTTTTAATAAT+AGG - Chr2:24335922-24335941 None:intergenic 0.0%
!! AGAGATAATAAAAAGAATTT+AGG - Chr2:24335953-24335972 None:intergenic 15.0%
!! TATTTAACAATTGAAAAAGA+GGG - Chr2:24337000-24337019 None:intergenic 15.0%
!! TTCAATTGATAAGATTAATT+TGG + Chr2:24337393-24337412 MsG0280008016.01.T01:CDS 15.0%
!!! GTTATCTAAAATTAAGTTAA+GGG - Chr2:24335497-24335516 None:intergenic 15.0%
!!! GTTGTTATTTTATAACATAA+AGG - Chr2:24335557-24335576 None:intergenic 15.0%
!!! TATACCATATATTTTTTTAG+AGG - Chr2:24335431-24335450 None:intergenic 15.0%
!!! TATTCTTTAAAAAAGTCAAA+TGG + Chr2:24336315-24336334 MsG0280008016.01.T01:intron 15.0%
!!! TGTTATCTAAAATTAAGTTA+AGG - Chr2:24335498-24335517 None:intergenic 15.0%
!!! TTGTCATTTTATAACATAAA+AGG + Chr2:24335717-24335736 MsG0280008016.01.T01:intron 15.0%
!! AAAATATATGGTATATCCTA+AGG + Chr2:24335436-24335455 MsG0280008016.01.T01:intron 20.0%
!! AAACTTGAATCAAAAATATG+TGG - Chr2:24337475-24337494 None:intergenic 20.0%
!! AAAGTTTGAGTAAAAAACTT+TGG + Chr2:24337281-24337300 MsG0280008016.01.T01:CDS 20.0%
!! AAATATATGGTATATCCTAA+GGG + Chr2:24335437-24335456 MsG0280008016.01.T01:intron 20.0%
!! AAGTTTGAGTAAAAAACTTT+GGG + Chr2:24337282-24337301 MsG0280008016.01.T01:CDS 20.0%
!! AATTGATATTAGACACAATA+AGG - Chr2:24337099-24337118 None:intergenic 20.0%
!! CTATTTAACAATTGAAAAAG+AGG - Chr2:24337001-24337020 None:intergenic 20.0%
!! GTTACCTAAAATTAAGTTAA+GGG + Chr2:24335777-24335796 MsG0280008016.01.T01:intron 20.0%
!! TAAGAATGTGTTTGTTTAAT+GGG + Chr2:24336241-24336260 MsG0280008016.01.T01:intron 20.0%
!! TATATTAGACTTCATCAAAA+TGG + Chr2:24335093-24335112 MsG0280008016.01.T01:CDS 20.0%
!! TGTTACCTAAAATTAAGTTA+AGG + Chr2:24335776-24335795 MsG0280008016.01.T01:intron 20.0%
!! TTAAGAATGTGTTTGTTTAA+TGG + Chr2:24336240-24336259 MsG0280008016.01.T01:intron 20.0%
!!! ATCAATTTAATGTCTGATTT+TGG + Chr2:24337112-24337131 MsG0280008016.01.T01:CDS 20.0%
!!! CCTAATTTAGAATGTTTAAA+GGG - Chr2:24336291-24336310 None:intergenic 20.0%
!!! CTCTTTTTCAATTGTTAAAT+AGG + Chr2:24336999-24337018 MsG0280008016.01.T01:CDS 20.0%
!!! GTTTTGATCCAAAATTTATT+TGG - Chr2:24336175-24336194 None:intergenic 20.0%
!!! TCAATGAAATTTGATTTTCT+AGG - Chr2:24335670-24335689 None:intergenic 20.0%
!!! TCCTAATTTAGAATGTTTAA+AGG - Chr2:24336292-24336311 None:intergenic 20.0%
!!! TGAGTTTGATGAATTTATTT+TGG - Chr2:24335618-24335637 None:intergenic 20.0%
!!! TTTCAATTTTTTCTATCCAA+TGG + Chr2:24336896-24336915 MsG0280008016.01.T01:CDS 20.0%
! AAAGGATTTGATTAACTCAT+TGG + Chr2:24337192-24337211 MsG0280008016.01.T01:CDS 25.0%
! ACAAGATAAAGTATATCCTA+AGG + Chr2:24335403-24335422 MsG0280008016.01.T01:intron 25.0%
! AGAAACCAAAATTGTTGATA+AGG - Chr2:24336694-24336713 None:intergenic 25.0%
! CAAGATAAAGTATATCCTAA+GGG + Chr2:24335404-24335423 MsG0280008016.01.T01:intron 25.0%
! CCCTTTAAACATTCTAAATT+AGG + Chr2:24336288-24336307 MsG0280008016.01.T01:intron 25.0%
! CTAAATAAATTAACTCCCTT+AGG - Chr2:24335455-24335474 None:intergenic 25.0%
! CTAAGGGAGTTAATTTATTT+AGG + Chr2:24335453-24335472 MsG0280008016.01.T01:intron 25.0%
! GAAAATTTACTCGAATGATA+GGG - Chr2:24336508-24336527 None:intergenic 25.0%
! TCGTTACCAAAAAAAGATAA+AGG - Chr2:24335528-24335547 None:intergenic 25.0%
! TGAAAATTTACTCGAATGAT+AGG - Chr2:24336509-24336528 None:intergenic 25.0%
! TGATCCAAAATTTATTTGGA+TGG - Chr2:24336171-24336190 None:intergenic 25.0%
!! AGAGAAACTCATTTTATAAG+AGG + Chr2:24335326-24335345 MsG0280008016.01.T01:intron 25.0%
!! CAAATTTTGGACATTCTAAA+AGG + Chr2:24334996-24335015 MsG0280008016.01.T01:CDS 25.0%
!! TAGTCCCTTAACTTAATTTT+AGG - Chr2:24335509-24335490 None:intergenic 25.0%
!! TCATACCTTATCAACAATTT+TGG + Chr2:24336686-24336705 MsG0280008016.01.T01:CDS 25.0%
!!! AGTTTTTGGGTTAATCAATT+GGG + Chr2:24337421-24337440 MsG0280008016.01.T01:CDS 25.0%
!!! TAATTTGGTGATGAGTTTTT+GGG + Chr2:24337408-24337427 MsG0280008016.01.T01:CDS 25.0%
!!! TTAATTTGGTGATGAGTTTT+TGG + Chr2:24337407-24337426 MsG0280008016.01.T01:CDS 25.0%
!!! TTTCGTCCTTTATCTTTTTT+TGG + Chr2:24335519-24335538 MsG0280008016.01.T01:intron 25.0%
!!! TTTCGTCCTTTATCTTTTTT+TGG - Chr2:24335538-24335519 None:intergenic 25.0%
AAGCTACTTCGTAAAATTCT+AGG - Chr2:24336962-24336981 None:intergenic 30.0%
ACATCGAATTAAGCAAACAA+TGG - Chr2:24337047-24337066 None:intergenic 30.0%
ACCTAAAACTACTACATTTG+TGG - Chr2:24336559-24336578 None:intergenic 30.0%
AGCTACTTCGTAAAATTCTA+GGG - Chr2:24336961-24336980 None:intergenic 30.0%
AGGTTCACGATTAACTTAAA+CGG + Chr2:24335352-24335371 MsG0280008016.01.T01:intron 30.0%
ATGAAGTCTAATATAGAGTC+TGG - Chr2:24335088-24335107 None:intergenic 30.0%
ATGAGTTCAAACAAACCTAA+AGG + Chr2:24335166-24335185 MsG0280008016.01.T01:intron 30.0%
CCAAAATTTATTTGGATGGT+TGG - Chr2:24336167-24336186 None:intergenic 30.0%
CTGATCAGGATTGAATATAA+TGG - Chr2:24336137-24336156 None:intergenic 30.0%
CTGATTGTATTTCAGCAAAA+GGG + Chr2:24335123-24335142 MsG0280008016.01.T01:intron 30.0%
GAGAAACAATTTCTTCAACA+TGG - Chr2:24336534-24336553 None:intergenic 30.0%
TAAAACTACTACATTTGTGG+CGG - Chr2:24336556-24336575 None:intergenic 30.0%
TAACGATGAAACAATATCAG+AGG - Chr2:24336930-24336949 None:intergenic 30.0%
TAGTATCTGATGAGTGAAAT+CGG - Chr2:24336614-24336633 None:intergenic 30.0%
TCATCATCAAAAAAGAACCT+TGG + Chr2:24336834-24336853 MsG0280008016.01.T01:CDS 30.0%
TCGTGACCAAAAAAAGATAA+AGG + Chr2:24335746-24335765 MsG0280008016.01.T01:intron 30.0%
TGATATTAGACACAATAAGG+TGG - Chr2:24337096-24337115 None:intergenic 30.0%
TGTAAATGTTAACTAATGCC+CGG - Chr2:24336411-24336430 None:intergenic 30.0%
TTACCCTTACGATATAATGT+AGG - Chr2:24335378-24335397 None:intergenic 30.0%
TTGAACCATTTCGTTATTCT+GGG + Chr2:24337575-24337594 MsG0280008016.01.T01:CDS 30.0%
TTGTTTGCTTAATTCGATGT+TGG + Chr2:24337047-24337066 MsG0280008016.01.T01:CDS 30.0%
! AACTGAATTAGGCTTTTCAA+GGG - Chr2:24336768-24336787 None:intergenic 30.0%
! ACTCATTTTATAAGAGGCTA+AGG + Chr2:24335332-24335351 MsG0280008016.01.T01:intron 30.0%
! ACTTTAGCAATAACCTTTTG+TGG - Chr2:24336877-24336896 None:intergenic 30.0%
! ATTTTCCCAGAATAACGAAA+TGG - Chr2:24337583-24337602 None:intergenic 30.0%
! CCAACCATCCAAATAAATTT+TGG + Chr2:24336164-24336183 MsG0280008016.01.T01:intron 30.0%
! CTTTAGCAATAACCTTTTGT+GGG - Chr2:24336876-24336895 None:intergenic 30.0%
! GGGTCCTCTAAAAAAATATA+TGG + Chr2:24335424-24335443 MsG0280008016.01.T01:intron 30.0%
! GTTTGAACTCATGGATATTT+TGG - Chr2:24335158-24335177 None:intergenic 30.0%
! TAGAATTTTACGAAGTAGCT+TGG + Chr2:24336961-24336980 MsG0280008016.01.T01:CDS 30.0%
! TTTAGCAATAACCTTTTGTG+GGG - Chr2:24336875-24336894 None:intergenic 30.0%
! TTTAGGTTTGTTTGAACTCA+TGG - Chr2:24335167-24335186 None:intergenic 30.0%
!! AAAACCATGGTTTTCTAAGA+AGG - Chr2:24336795-24336814 None:intergenic 30.0%
!! CAAGTTTGTTTTCAGCTTTA+AGG + Chr2:24337612-24337631 MsG0280008016.01.T01:CDS 30.0%
!!! AAGCTGTTTTTCAAGCATTT+AGG + Chr2:24337347-24337366 MsG0280008016.01.T01:CDS 30.0%
!!! GAATACTTTTGGAATTGCTT+TGG + Chr2:24337249-24337268 MsG0280008016.01.T01:CDS 30.0%
!!! GAGTTTTTGGGTTAATCAAT+TGG + Chr2:24337420-24337439 MsG0280008016.01.T01:CDS 30.0%
!!! TATTTTTTTAGAGGACCCTT+AGG - Chr2:24335422-24335441 None:intergenic 30.0%
AAGAAGGTGAGAACTGAATT+AGG - Chr2:24336779-24336798 None:intergenic 35.0%
AAGTCTAATATAGAGTCTGG+TGG - Chr2:24335085-24335104 None:intergenic 35.0%
AGACAACAAAAGCAAGTGTA+AGG + Chr2:24336204-24336223 MsG0280008016.01.T01:intron 35.0%
AGTATCTGATGAGTGAAATC+GGG - Chr2:24336613-24336632 None:intergenic 35.0%
AGTCTAATATAGAGTCTGGT+GGG - Chr2:24335084-24335103 None:intergenic 35.0%
ATAAGGTATGATACAGCGAA+TGG - Chr2:24336677-24336696 None:intergenic 35.0%
ATCTTGTGATGAAAGGTTTG+AGG + Chr2:24337533-24337552 MsG0280008016.01.T01:CDS 35.0%
ATTAGACACAATAAGGTGGT+AGG - Chr2:24337092-24337111 None:intergenic 35.0%
ATTGAAGTCAACATGAAACC+AGG - Chr2:24337379-24337398 None:intergenic 35.0%
CAAGTGTTCATCTTCTAACA+AGG - Chr2:24335231-24335250 None:intergenic 35.0%
CTGCAATATCTTGTGATGAA+AGG + Chr2:24337526-24337545 MsG0280008016.01.T01:CDS 35.0%
CTTGTTAGAAGATGAACACT+TGG + Chr2:24335229-24335248 MsG0280008016.01.T01:intron 35.0%
GCAGAAAACAATTGTTCCAA+GGG + Chr2:24337495-24337514 MsG0280008016.01.T01:CDS 35.0%
GCATACACAAAAGAGAACAA+TGG + Chr2:24337682-24337701 MsG0280008016.01.T01:CDS 35.0%
GCTGATTGTATTTCAGCAAA+AGG + Chr2:24335122-24335141 MsG0280008016.01.T01:intron 35.0%
GGATTTGATTAACTCATTGG+AGG + Chr2:24337195-24337214 MsG0280008016.01.T01:CDS 35.0%
GTAAATGTTAACTAATGCCC+GGG - Chr2:24336410-24336429 None:intergenic 35.0%
GTTGAACCATTTCGTTATTC+TGG + Chr2:24337574-24337593 MsG0280008016.01.T01:CDS 35.0%
TAACAAGGAAGAAGGAACAA+AGG - Chr2:24335216-24335235 None:intergenic 35.0%
TAAGGTATGATACAGCGAAT+GGG - Chr2:24336676-24336695 None:intergenic 35.0%
TCATCTTCTAACAAGGAAGA+AGG - Chr2:24335224-24335243 None:intergenic 35.0%
TCCTTTGAACCAAATGTAGA+TGG - Chr2:24337178-24337197 None:intergenic 35.0%
TGCAGAAAACAATTGTTCCA+AGG + Chr2:24337494-24337513 MsG0280008016.01.T01:CDS 35.0%
TGGAGGAAGTTAAGAGTTTA+GGG + Chr2:24337212-24337231 MsG0280008016.01.T01:CDS 35.0%
TTAGACACAATAAGGTGGTA+GGG - Chr2:24337091-24337110 None:intergenic 35.0%
TTGGAGGAAGTTAAGAGTTT+AGG + Chr2:24337211-24337230 MsG0280008016.01.T01:CDS 35.0%
! AGTTGATGCCCTTAAGAAAT+GGG + Chr2:24337312-24337331 MsG0280008016.01.T01:CDS 35.0%
! CAAAGGATAGTGAAAAGTGT+TGG - Chr2:24335199-24335218 None:intergenic 35.0%
! GAACTGAATTAGGCTTTTCA+AGG - Chr2:24336769-24336788 None:intergenic 35.0%
! GCCACAAATGTAGTAGTTTT+AGG + Chr2:24336555-24336574 MsG0280008016.01.T01:CDS 35.0%
! TGTCAAGCAAGTTCTGATAA+TGG + Chr2:24335865-24335884 MsG0280008016.01.T01:intron 35.0%
! TTTGGGTTAATCAATTGGGT+GGG + Chr2:24337425-24337444 MsG0280008016.01.T01:CDS 35.0%
!! AAGTTGATGCCCTTAAGAAA+TGG + Chr2:24337311-24337330 MsG0280008016.01.T01:CDS 35.0%
!! ATTCCAAAAGTATTCGTCCA+AGG - Chr2:24337244-24337263 None:intergenic 35.0%
!! TAGGTTCTTTAAAACCGACA+AGG + Chr2:24337018-24337037 MsG0280008016.01.T01:CDS 35.0%
!!! ATGGTTTTGAATCAAGGTGA+AGG - Chr2:24335059-24335078 None:intergenic 35.0%
!!! GTTTGTTTTCAGCTTTAAGG+AGG + Chr2:24337615-24337634 MsG0280008016.01.T01:CDS 35.0%
!!! TGGGTAATGGTTTTGAATCA+AGG - Chr2:24335065-24335084 None:intergenic 35.0%
!!! TTTTGGGTTAATCAATTGGG+TGG + Chr2:24337424-24337443 MsG0280008016.01.T01:CDS 35.0%
ACGGCCTACATTATATCGTA+AGG + Chr2:24335371-24335390 MsG0280008016.01.T01:intron 40.0%
AGACCAACCCCATTTCTTAA+GGG - Chr2:24337323-24337342 None:intergenic 40.0%
CAAAGCTTTCAACAACAAGC+AGG + Chr2:24336730-24336749 MsG0280008016.01.T01:CDS 40.0%
CAAGTTACACATTCGGTAGA+TGG - Chr2:24337718-24337737 None:intergenic 40.0%
CGGCCTACATTATATCGTAA+GGG + Chr2:24335372-24335391 MsG0280008016.01.T01:intron 40.0%
CTCACCTTCTTAGAAAACCA+TGG + Chr2:24336788-24336807 MsG0280008016.01.T01:CDS 40.0%
GCAAACAATGGTATCCTTGT+CGG - Chr2:24337035-24337054 None:intergenic 40.0%
GTGAAGGTTACTATCAGGAT+AGG - Chr2:24335043-24335062 None:intergenic 40.0%
GTTGATGCCCTTAAGAAATG+GGG + Chr2:24337313-24337332 MsG0280008016.01.T01:CDS 40.0%
TAAGACCTTAACAAGTCTCC+CGG + Chr2:24336389-24336408 MsG0280008016.01.T01:intron 40.0%
TAGACCAACCCCATTTCTTA+AGG - Chr2:24337324-24337343 None:intergenic 40.0%
TCAAGGTGAAGGTTACTATC+AGG - Chr2:24335048-24335067 None:intergenic 40.0%
TGGTTGGAAGATAACTGATC+AGG - Chr2:24336151-24336170 None:intergenic 40.0%
TGTTGTCGTGTGAAAAACCA+TGG - Chr2:24336808-24336827 None:intergenic 40.0%
! AACAATTGTTCCAAGGGCAT+TGG + Chr2:24337501-24337520 MsG0280008016.01.T01:CDS 40.0%
! ACATTCTAAAAGGACCTGTC+TGG + Chr2:24335006-24335025 MsG0280008016.01.T01:CDS 40.0%
! AGAGTTTAGGGTTTCATCCT+TGG + Chr2:24337224-24337243 MsG0280008016.01.T01:CDS 40.0%
! AGTGTTGGATGGATACCTTT+AGG - Chr2:24335184-24335203 None:intergenic 40.0%
! ATATAGAGTCTGGTGGGTAA+TGG - Chr2:24335078-24335097 None:intergenic 40.0%
! ATTCCTTGGCACTTGCTAAA+AGG + Chr2:24337449-24337468 MsG0280008016.01.T01:CDS 40.0%
! CATCCTTGGACGAATACTTT+TGG + Chr2:24337238-24337257 MsG0280008016.01.T01:CDS 40.0%
! GCCATCTACATTTGGTTCAA+AGG + Chr2:24337174-24337193 MsG0280008016.01.T01:CDS 40.0%
! GTCTTGTTAACGATTATCCC+GGG + Chr2:24336263-24336282 MsG0280008016.01.T01:intron 40.0%
! TCAGAACTTGCTTGACACTT+AGG - Chr2:24335862-24335881 None:intergenic 40.0%
!! AAAGCTTTGAGAGCAGATTG+AGG - Chr2:24336719-24336738 None:intergenic 40.0%
!! GGATAGTGAAAAGTGTTGGA+TGG - Chr2:24335195-24335214 None:intergenic 40.0%
!! GGTCTTGTTAACGATTATCC+CGG + Chr2:24336262-24336281 MsG0280008016.01.T01:intron 40.0%
!! TAGAATGTTTAAAGGGTGCC+CGG - Chr2:24336284-24336303 None:intergenic 40.0%
AAGACCTTAACAAGTCTCCC+GGG + Chr2:24336390-24336409 MsG0280008016.01.T01:intron 45.0%
ACACATTCGGTAGATGGCAT+TGG - Chr2:24337712-24337731 None:intergenic 45.0%
ACTATCAGGATAGGTGTCTC+TGG - Chr2:24335034-24335053 None:intergenic 45.0%
ATGCCCTTAAGAAATGGGGT+TGG + Chr2:24337317-24337336 MsG0280008016.01.T01:CDS 45.0%
CAGTCAAGGCCATCTACATT+TGG + Chr2:24337166-24337185 MsG0280008016.01.T01:CDS 45.0%
GGCTGTGATGAAAGTAACCA+AGG - Chr2:24336854-24336873 None:intergenic 45.0%
TATTGCAGAACCAATGCCCT+TGG - Chr2:24337514-24337533 None:intergenic 45.0%
TCAATTGGGTGGGAATTCCT+TGG + Chr2:24337435-24337454 MsG0280008016.01.T01:CDS 45.0%
TTTCATCACAGCCCCACAAA+AGG + Chr2:24336861-24336880 MsG0280008016.01.T01:CDS 45.0%
! ATCGGGAACAACAAGTGTTG+AGG - Chr2:24336596-24336615 None:intergenic 45.0%
! CAAGCATTTAGGATGCATCC+TGG + Chr2:24337358-24337377 MsG0280008016.01.T01:CDS 45.0%
!! AGAATGTTTAAAGGGTGCCC+GGG - Chr2:24336283-24336302 None:intergenic 45.0%
!! ATCAGAGGAAGAAGCACCAT+TGG - Chr2:24336915-24336934 None:intergenic 45.0%
!! TCGGGAACAACAAGTGTTGA+GGG - Chr2:24336595-24336614 None:intergenic 45.0%
!! TTTACATTATTATAAATAAA+TGG - Chr2:24335821-24335840 None:intergenic 5.0%
!!! TATATTTAAAAAAGTTTTTT+GGG - Chr2:24335976-24335995 None:intergenic 5.0%
!!! TTATATTTAAAAAAGTTTTT+TGG - Chr2:24335977-24335996 None:intergenic 5.0%
TCGAGCGAAACAGAGTGGAA+TGG - Chr2:24336112-24336131 None:intergenic 50.0%
TGGAATCGAGCGAAACAGAG+TGG - Chr2:24336117-24336136 None:intergenic 50.0%
TTGCTAGCTCGCTTCAGTCA+AGG + Chr2:24337152-24337171 MsG0280008016.01.T01:CDS 50.0%
! TGGCCTTTTAGCAAGTGCCA+AGG - Chr2:24337455-24337474 None:intergenic 50.0%
!! AATGCCCGGGAGACTTGTTA+AGG - Chr2:24336397-24336416 None:intergenic 50.0%
!! AGGTGTCTCTGGAACCAGAC+AGG - Chr2:24335023-24335042 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 24334986 24337741 24334986 ID=MsG0280008016.01;Name=MsG0280008016.01
Chr2 mRNA 24334986 24337741 24334986 ID=MsG0280008016.01.T01;Parent=MsG0280008016.01;Name=MsG0280008016.01.T01;_AED=0.48;_eAED=0.53;_QI=0|0|0|1|0|0|2|0|455
Chr2 exon 24334986 24335114 24334986 ID=MsG0280008016.01.T01:exon:13953;Parent=MsG0280008016.01.T01
Chr2 exon 24336503 24337741 24336503 ID=MsG0280008016.01.T01:exon:13954;Parent=MsG0280008016.01.T01
Chr2 CDS 24334986 24335114 24334986 ID=MsG0280008016.01.T01:cds;Parent=MsG0280008016.01.T01
Chr2 CDS 24336503 24337741 24336503 ID=MsG0280008016.01.T01:cds;Parent=MsG0280008016.01.T01
Gene Sequence

>MsG0280008016.01.T01

ATGGTGATGGCAAATTTTGGACATTCTAAAAGGACCTGTCTGGTTCCAGAGACACCTATCCTGATAGTAACCTTCACCTTGATTCAAAACCATTACCCACCAGACTCTATATTAGACTTCATCAAAATGAACCCTATCATTCGAGTAAATTTTCAATCCATGTTGAAGAAATTGTTTCTCCGCCACAAATGTAGTAGTTTTAGGTCTCATCACAACAACCCTCAACACTTGTTGTTCCCGATTTCACTCATCAGATACTACAACACTACAATAACAACAACATCTGAATCAAACACACACCCATTCGCTGTATCATACCTTATCAACAATTTTGGTTTCTCACCTCAATCTGCTCTCAAAGCTTTCAACAACAAGCAGGTTCGTTTCAATACCCTTGAAAAGCCTAATTCAGTTCTCACCTTCTTAGAAAACCATGGTTTTTCACACGACAACATACGCATCATCATCAAAAAAGAACCTTGGTTACTTTCATCACAGCCCCACAAAAGGTTATTGCTAAAGTTTCAATTTTTTCTATCCAATGGTGCTTCTTCCTCTGATATTGTTTCATCGTTAACTTCAAACCCTAGAATTTTACGAAGTAGCTTGGAGAAACAAATAATCCCTCTTTTTCAATTGTTAAATAGGTTCTTTAAAACCGACAAGGATACCATTGTTTGCTTAATTCGATGTTGGACTGCACTTGCTATTAATCCCTACCACCTTATTGTGTCTAATATCAATTTAATGTCTGATTTTGGAGTTTCTGATTCTGTCATTGCTAGCTCGCTTCAGTCAAGGCCATCTACATTTGGTTCAAAGGATTTGATTAACTCATTGGAGGAAGTTAAGAGTTTAGGGTTTCATCCTTGGACGAATACTTTTGGAATTGCTTTGGCAGCTAAGAAAAGTTTGAGTAAAAAACTTTGGGATGAGAAAGTTGATGCCCTTAAGAAATGGGGTTGGTCTAATGAAGCTGTTTTTCAAGCATTTAGGATGCATCCTGGTTTCATGTTGACTTCAATTGATAAGATTAATTTGGTGATGAGTTTTTGGGTTAATCAATTGGGTGGGAATTCCTTGGCACTTGCTAAAAGGCCACATATTTTTGATTCAAGTTTGCAGAAAACAATTGTTCCAAGGGCATTGGTTCTGCAATATCTTGTGATGAAAGGTTTGAGGAAAAAGAATGCAAGCTTAGTTGAACCATTTCGTTATTCTGGGAAAATGTTCTTGAGCAAGTTTGTTTTCAGCTTTAAGGAGGAGTCTGATTATCTATTAAAGTTATATGACGAAAAAATGAAACTTGCATACACAAAAGAGAACAATGGCATGCCAATGCCATCTACCGAATGTGTAACTTGTTAA
Protein sequence

>MsG0280008016.01.T01

MVMANFGHSKRTCLVPETPILIVTFTLIQNHYPPDSILDFIKMNPIIRVNFQSMLKKLFLRHKCSSFRSHHNNPQHLLFPISLIRYYNTTITTTSESNTHPFAVSYLINNFGFSPQSALKAFNNKQVRFNTLEKPNSVLTFLENHGFSHDNIRIIIKKEPWLLSSQPHKRLLLKFQFFLSNGASSSDIVSSLTSNPRILRSSLEKQIIPLFQLLNRFFKTDKDTIVCLIRCWTALAINPYHLIVSNINLMSDFGVSDSVIASSLQSRPSTFGSKDLINSLEEVKSLGFHPWTNTFGIALAAKKSLSKKLWDEKVDALKKWGWSNEAVFQAFRMHPGFMLTSIDKINLVMSFWVNQLGGNSLALAKRPHIFDSSLQKTIVPRALVLQYLVMKGLRKKNASLVEPFRYSGKMFLSKFVFSFKEESDYLLKLYDEKMKLAYTKENNGMPMPSTECVTC*