Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008093.01.T01 | GAU41275.1 | 71.023 | 176 | 49 | 1 | 1 | 174 | 1 | 176 | 9.90E-83 | 255 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008093.01.T01 | Q9FJK3 | 53.704 | 162 | 72 | 2 | 1 | 161 | 1 | 160 | 7.76E-54 | 176 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008093.01.T01 | A0A2Z6NZ76 | 71.023 | 176 | 49 | 1 | 1 | 174 | 1 | 176 | 4.73e-83 | 255 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0280008093.01.T01 | TF | MADS-M-type |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008093.01.T01 | MTR_8g036130 | 69.189 | 185 | 48 | 3 | 1 | 177 | 1 | 184 | 3.23e-84 | 249 |
| MsG0280008093.01.T01 | MTR_2g035610 | 92.623 | 122 | 9 | 0 | 1 | 122 | 1 | 122 | 1.32e-74 | 220 |
| MsG0280008093.01.T01 | MTR_4g031910 | 65.556 | 180 | 57 | 2 | 1 | 176 | 1 | 179 | 2.69e-73 | 221 |
| MsG0280008093.01.T01 | MTR_4g032620 | 63.580 | 162 | 59 | 0 | 1 | 162 | 1 | 162 | 9.56e-70 | 211 |
| MsG0280008093.01.T01 | MTR_3g065100 | 64.375 | 160 | 56 | 1 | 1 | 160 | 1 | 159 | 2.81e-69 | 211 |
| MsG0280008093.01.T01 | MTR_3g466980 | 61.538 | 156 | 59 | 1 | 1 | 156 | 1 | 155 | 5.24e-69 | 209 |
| MsG0280008093.01.T01 | MTR_4g032290 | 62.778 | 180 | 62 | 2 | 1 | 176 | 1 | 179 | 1.42e-64 | 198 |
| MsG0280008093.01.T01 | MTR_4g032260 | 69.032 | 155 | 48 | 0 | 1 | 155 | 1 | 155 | 3.82e-63 | 192 |
| MsG0280008093.01.T01 | MTR_3g466830 | 51.572 | 159 | 76 | 1 | 3 | 161 | 2 | 159 | 4.63e-56 | 174 |
| MsG0280008093.01.T01 | MTR_4g063790 | 44.586 | 157 | 87 | 0 | 1 | 157 | 1 | 157 | 2.26e-48 | 155 |
| MsG0280008093.01.T01 | MTR_3g466890 | 44.937 | 158 | 86 | 1 | 3 | 160 | 2 | 158 | 1.92e-46 | 150 |
| MsG0280008093.01.T01 | MTR_3g467080 | 41.935 | 155 | 90 | 0 | 1 | 155 | 1 | 155 | 1.25e-42 | 140 |
| MsG0280008093.01.T01 | MTR_2g016210 | 42.405 | 158 | 91 | 0 | 1 | 158 | 1 | 158 | 1.36e-42 | 143 |
| MsG0280008093.01.T01 | MTR_5g075380 | 41.250 | 160 | 93 | 1 | 1 | 160 | 1 | 159 | 2.71e-42 | 139 |
| MsG0280008093.01.T01 | MTR_3g031100 | 42.675 | 157 | 90 | 0 | 1 | 157 | 1 | 157 | 3.61e-42 | 142 |
| MsG0280008093.01.T01 | MTR_2g035590 | 79.381 | 97 | 20 | 0 | 74 | 170 | 1 | 97 | 4.22e-40 | 132 |
| MsG0280008093.01.T01 | MTR_5g047580 | 42.581 | 155 | 87 | 2 | 1 | 155 | 1 | 153 | 1.57e-39 | 132 |
| MsG0280008093.01.T01 | MTR_1g090697 | 39.241 | 158 | 94 | 2 | 1 | 158 | 1 | 156 | 2.13e-37 | 127 |
| MsG0280008093.01.T01 | MTR_3g466900 | 41.085 | 129 | 75 | 1 | 32 | 160 | 2 | 129 | 3.05e-35 | 120 |
| MsG0280008093.01.T01 | MTR_3g031240 | 34.810 | 158 | 103 | 0 | 1 | 158 | 1 | 158 | 3.24e-35 | 122 |
| MsG0280008093.01.T01 | MTR_7g011950 | 43.506 | 154 | 85 | 2 | 1 | 154 | 1 | 152 | 3.92e-35 | 121 |
| MsG0280008093.01.T01 | MTR_1g077390 | 39.610 | 154 | 91 | 2 | 1 | 154 | 1 | 152 | 2.58e-34 | 119 |
| MsG0280008093.01.T01 | MTR_1g090710 | 36.076 | 158 | 100 | 1 | 1 | 158 | 1 | 157 | 3.64e-34 | 119 |
| MsG0280008093.01.T01 | MTR_1g077320 | 42.857 | 154 | 86 | 2 | 1 | 154 | 1 | 152 | 3.81e-34 | 119 |
| MsG0280008093.01.T01 | MTR_1g090783 | 37.821 | 156 | 95 | 2 | 3 | 158 | 2 | 155 | 4.12e-34 | 118 |
| MsG0280008093.01.T01 | MTR_3g466930 | 41.860 | 129 | 74 | 1 | 32 | 160 | 2 | 129 | 4.55e-34 | 117 |
| MsG0280008093.01.T01 | MTR_3g465410 | 41.085 | 129 | 76 | 0 | 29 | 157 | 1 | 129 | 2.51e-33 | 116 |
| MsG0280008093.01.T01 | MTR_7g055800 | 53.782 | 119 | 27 | 2 | 1 | 119 | 1 | 91 | 6.34e-33 | 113 |
| MsG0280008093.01.T01 | MTR_4g028720 | 40.909 | 154 | 89 | 2 | 1 | 154 | 1 | 152 | 2.35e-32 | 114 |
| MsG0280008093.01.T01 | MTR_1g077300 | 42.208 | 154 | 85 | 2 | 1 | 154 | 1 | 150 | 2.76e-32 | 114 |
| MsG0280008093.01.T01 | MTR_1g084950 | 40.645 | 155 | 90 | 2 | 1 | 155 | 1 | 153 | 3.52e-32 | 113 |
| MsG0280008093.01.T01 | MTR_7g055790 | 58.559 | 111 | 28 | 1 | 1 | 111 | 59 | 151 | 3.27e-30 | 108 |
| MsG0280008093.01.T01 | MTR_4g028800 | 70.833 | 72 | 19 | 1 | 1 | 72 | 1 | 70 | 1.69e-29 | 104 |
| MsG0280008093.01.T01 | MTR_2g035580 | 36.774 | 155 | 97 | 1 | 1 | 155 | 1 | 154 | 6.59e-27 | 100 |
| MsG0280008093.01.T01 | MTR_7g055940 | 51.887 | 106 | 23 | 2 | 1 | 106 | 1 | 78 | 2.46e-25 | 93.6 |
| MsG0280008093.01.T01 | MTR_5g047560 | 32.903 | 155 | 86 | 3 | 1 | 155 | 1 | 137 | 8.43e-22 | 86.7 |
| MsG0280008093.01.T01 | MTR_3g093900 | 34.646 | 127 | 83 | 0 | 3 | 129 | 6 | 132 | 3.97e-21 | 90.1 |
| MsG0280008093.01.T01 | MTR_4g019670 | 36.364 | 132 | 81 | 1 | 1 | 129 | 1 | 132 | 1.72e-20 | 88.2 |
| MsG0280008093.01.T01 | MTR_7g106510 | 35.606 | 132 | 82 | 1 | 1 | 129 | 1 | 132 | 2.94e-20 | 87.8 |
| MsG0280008093.01.T01 | MTR_4g084780 | 61.538 | 52 | 20 | 0 | 74 | 125 | 1 | 52 | 9.56e-15 | 68.6 |
| MsG0280008093.01.T01 | MTR_6g018920 | 25.000 | 160 | 105 | 5 | 1 | 154 | 1 | 151 | 2.79e-12 | 64.3 |
| MsG0280008093.01.T01 | MTR_1g114730 | 25.899 | 139 | 86 | 5 | 1 | 127 | 1 | 134 | 2.24e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008093.01.T01 | AT5G48670 | 53.704 | 162 | 72 | 2 | 1 | 161 | 1 | 160 | 7.91e-55 | 176 |
| MsG0280008093.01.T01 | AT5G26630 | 47.904 | 167 | 86 | 1 | 1 | 167 | 1 | 166 | 2.11e-45 | 149 |
| MsG0280008093.01.T01 | AT1G65300 | 41.818 | 165 | 95 | 1 | 3 | 167 | 2 | 165 | 4.12e-40 | 137 |
| MsG0280008093.01.T01 | AT1G65330 | 40.000 | 180 | 107 | 1 | 3 | 182 | 2 | 180 | 6.15e-40 | 137 |
| MsG0280008093.01.T01 | AT5G26650 | 43.038 | 158 | 89 | 1 | 4 | 161 | 2 | 158 | 1.36e-39 | 138 |
| MsG0280008093.01.T01 | AT5G27960 | 43.038 | 158 | 89 | 1 | 4 | 161 | 2 | 158 | 1.79e-39 | 137 |
| MsG0280008093.01.T01 | AT1G31630 | 34.302 | 172 | 111 | 2 | 3 | 174 | 2 | 171 | 4.27e-34 | 123 |
| MsG0280008093.01.T01 | AT5G27810 | 51.613 | 93 | 45 | 0 | 29 | 121 | 1 | 93 | 2.88e-32 | 112 |
| MsG0280008093.01.T01 | AT3G05860 | 38.415 | 164 | 96 | 2 | 1 | 162 | 1 | 161 | 3.25e-32 | 115 |
| MsG0280008093.01.T01 | AT3G05860 | 38.415 | 164 | 96 | 2 | 1 | 162 | 1 | 161 | 2.17e-31 | 114 |
| MsG0280008093.01.T01 | AT3G05860 | 38.415 | 164 | 96 | 2 | 1 | 162 | 1 | 161 | 2.66e-31 | 114 |
| MsG0280008093.01.T01 | AT1G31640 | 37.748 | 151 | 92 | 2 | 3 | 153 | 2 | 150 | 5.51e-30 | 114 |
| MsG0280008093.01.T01 | AT5G26580 | 35.404 | 161 | 89 | 2 | 1 | 161 | 1 | 146 | 2.49e-27 | 105 |
| MsG0280008093.01.T01 | AT2G28700 | 36.957 | 138 | 85 | 1 | 1 | 136 | 1 | 138 | 7.87e-27 | 104 |
| MsG0280008093.01.T01 | AT1G22590 | 29.448 | 163 | 110 | 2 | 1 | 163 | 1 | 158 | 3.27e-20 | 83.2 |
| MsG0280008093.01.T01 | AT2G40210 | 37.795 | 127 | 79 | 0 | 1 | 127 | 1 | 127 | 2.82e-19 | 84.3 |
| MsG0280008093.01.T01 | AT5G06500 | 30.519 | 154 | 105 | 1 | 1 | 154 | 1 | 152 | 1.09e-18 | 80.9 |
| MsG0280008093.01.T01 | AT5G55690 | 39.362 | 94 | 50 | 2 | 1 | 87 | 1 | 94 | 2.85e-13 | 66.6 |
| MsG0280008093.01.T01 | AT5G55690 | 39.362 | 94 | 50 | 2 | 1 | 87 | 1 | 94 | 2.85e-13 | 66.6 |
| MsG0280008093.01.T01 | AT5G58890 | 40.179 | 112 | 58 | 4 | 1 | 105 | 1 | 110 | 2.24e-12 | 64.3 |
| MsG0280008093.01.T01 | AT2G15660 | 24.713 | 174 | 111 | 5 | 11 | 180 | 18 | 175 | 6.28e-12 | 62.8 |
Find 36 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCAATATCCTTCAAATTT+TGG | 0.124846 | 2:-25401402 | None:intergenic |
| TTCTTGTTCTTCTCTAATTC+AGG | 0.242401 | 2:-25401193 | None:intergenic |
| ATGAACCTCAACCTCATATT+TGG | 0.258532 | 2:+25401122 | MsG0280008093.01.T01:CDS |
| CACAATCATGACTAGGAAAA+AGG | 0.298378 | 2:+25400964 | None:intergenic |
| TGAAGCATGTGCTATAGTTC+AGG | 0.354873 | 2:+25401093 | MsG0280008093.01.T01:CDS |
| TTGTTGGTGGTGGTGTCATT+TGG | 0.382506 | 2:-25401499 | None:intergenic |
| ATAAGGTGCTCTCAAAATTC+AGG | 0.411332 | 2:+25401164 | MsG0280008093.01.T01:CDS |
| GTTGATTAACCAAAATTTGA+AGG | 0.422385 | 2:+25401393 | MsG0280008093.01.T01:CDS |
| GAAGGATATTGATAGAAGAT+TGG | 0.427774 | 2:+25401411 | MsG0280008093.01.T01:CDS |
| TCAAGTCATGACTACTCAAT+TGG | 0.431051 | 2:+25401462 | MsG0280008093.01.T01:CDS |
| TTTGCGTCATGAATGTCTCT+TGG | 0.452946 | 2:-25401225 | None:intergenic |
| TTGGAAGAAGTGGATAATAA+TGG | 0.455688 | 2:+25401430 | MsG0280008093.01.T01:CDS |
| ATGTTTAATCTATAAATTGT+TGG | 0.458771 | 2:-25401515 | None:intergenic |
| ATCACCATGGGGAGTTCATA+AGG | 0.462505 | 2:+25401147 | MsG0280008093.01.T01:CDS |
| GGAAATCAACCTATAAGAAA+AGG | 0.476350 | 2:+25401020 | MsG0280008093.01.T01:CDS |
| GATGAACTGAGCACGCTTTG+TGG | 0.480014 | 2:+25401067 | MsG0280008093.01.T01:CDS |
| TTGATTTGAATGACCTTGCT+TGG | 0.489187 | 2:+25401371 | MsG0280008093.01.T01:CDS |
| AATCTATAAATTGTTGGTGG+TGG | 0.494608 | 2:-25401509 | None:intergenic |
| CTCATATTTGGCCATCACCA+TGG | 0.499740 | 2:+25401134 | MsG0280008093.01.T01:CDS |
| TCATATTTGGCCATCACCAT+GGG | 0.503042 | 2:+25401135 | MsG0280008093.01.T01:CDS |
| GAAGCATGTGCTATAGTTCA+GGG | 0.519428 | 2:+25401094 | MsG0280008093.01.T01:CDS |
| TGATAGAAGATTGGAAGAAG+TGG | 0.523326 | 2:+25401420 | MsG0280008093.01.T01:CDS |
| CATGGTGATGGCCAAATATG+AGG | 0.531321 | 2:-25401133 | None:intergenic |
| TCATTGTCAATGACGCTGCT+CGG | 0.532000 | 2:+25400999 | MsG0280008093.01.T01:CDS |
| AGAGACATTCATGACGCAAA+GGG | 0.534224 | 2:+25401228 | MsG0280008093.01.T01:CDS |
| TTTGGTTAATCAACCAAGCA+AGG | 0.538786 | 2:-25401384 | None:intergenic |
| TTATGAACTCCCCATGGTGA+TGG | 0.560046 | 2:-25401145 | None:intergenic |
| TGAGTAGTCATGACTTGACT+TGG | 0.575659 | 2:-25401457 | None:intergenic |
| TTTAATCTATAAATTGTTGG+TGG | 0.576984 | 2:-25401512 | None:intergenic |
| CACAACTCACAATCATGACT+AGG | 0.590575 | 2:+25400957 | None:intergenic |
| AAGAGACATTCATGACGCAA+AGG | 0.614539 | 2:+25401227 | MsG0280008093.01.T01:CDS |
| GATGGCCAAATATGAGGTTG+AGG | 0.620009 | 2:-25401127 | None:intergenic |
| AAAACTAAGGAAGGGAAACA+GGG | 0.623030 | 2:+25401276 | MsG0280008093.01.T01:CDS |
| ATATGAGGTTGAGGTTCATG+AGG | 0.635051 | 2:-25401118 | None:intergenic |
| AGCACCTTATGAACTCCCCA+TGG | 0.654106 | 2:-25401151 | None:intergenic |
| CATATTTGGCCATCACCATG+GGG | 0.723900 | 2:+25401136 | MsG0280008093.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTATTCATCCTTTTCTTAT+AGG | - | Chr2:25401032-25401051 | None:intergenic | 20.0% |
| !!! | TATCAATATCCTTCAAATTT+TGG | - | Chr2:25401405-25401424 | None:intergenic | 20.0% |
| ! | AAAGTGAAAAAACTAAGGAA+GGG | + | Chr2:25401268-25401287 | MsG0280008093.01.T01:CDS | 25.0% |
| ! | GTTGATTAACCAAAATTTGA+AGG | + | Chr2:25401393-25401412 | MsG0280008093.01.T01:CDS | 25.0% |
| AGGAGAAAGTGAAAAAACTA+AGG | + | Chr2:25401263-25401282 | MsG0280008093.01.T01:CDS | 30.0% | |
| GAAAGTGAAAAAACTAAGGA+AGG | + | Chr2:25401267-25401286 | MsG0280008093.01.T01:CDS | 30.0% | |
| GAAGGATATTGATAGAAGAT+TGG | + | Chr2:25401411-25401430 | MsG0280008093.01.T01:CDS | 30.0% | |
| GGAAATCAACCTATAAGAAA+AGG | + | Chr2:25401020-25401039 | MsG0280008093.01.T01:CDS | 30.0% | |
| TTCTTGTTCTTCTCTAATTC+AGG | - | Chr2:25401196-25401215 | None:intergenic | 30.0% | |
| TTGGAAGAAGTGGATAATAA+TGG | + | Chr2:25401430-25401449 | MsG0280008093.01.T01:CDS | 30.0% | |
| AAAAACTAAGGAAGGGAAAC+AGG | + | Chr2:25401275-25401294 | MsG0280008093.01.T01:CDS | 35.0% | |
| AAAACTAAGGAAGGGAAACA+GGG | + | Chr2:25401276-25401295 | MsG0280008093.01.T01:CDS | 35.0% | |
| ATGAACCTCAACCTCATATT+TGG | + | Chr2:25401122-25401141 | MsG0280008093.01.T01:CDS | 35.0% | |
| TCAAGTCATGACTACTCAAT+TGG | + | Chr2:25401462-25401481 | MsG0280008093.01.T01:CDS | 35.0% | |
| TGATAGAAGATTGGAAGAAG+TGG | + | Chr2:25401420-25401439 | MsG0280008093.01.T01:CDS | 35.0% | |
| TTTGGTTAATCAACCAAGCA+AGG | - | Chr2:25401387-25401406 | None:intergenic | 35.0% | |
| ! | ATAAGGTGCTCTCAAAATTC+AGG | + | Chr2:25401164-25401183 | MsG0280008093.01.T01:CDS | 35.0% |
| ! | TTGATTTGAATGACCTTGCT+TGG | + | Chr2:25401371-25401390 | MsG0280008093.01.T01:CDS | 35.0% |
| AAGAGACATTCATGACGCAA+AGG | + | Chr2:25401227-25401246 | MsG0280008093.01.T01:CDS | 40.0% | |
| AGAGACATTCATGACGCAAA+GGG | + | Chr2:25401228-25401247 | MsG0280008093.01.T01:CDS | 40.0% | |
| ATATGAGGTTGAGGTTCATG+AGG | - | Chr2:25401121-25401140 | None:intergenic | 40.0% | |
| GAAGCATGTGCTATAGTTCA+GGG | + | Chr2:25401094-25401113 | MsG0280008093.01.T01:CDS | 40.0% | |
| TCATATTTGGCCATCACCAT+GGG | + | Chr2:25401135-25401154 | MsG0280008093.01.T01:CDS | 40.0% | |
| TGAAGCATGTGCTATAGTTC+AGG | + | Chr2:25401093-25401112 | MsG0280008093.01.T01:CDS | 40.0% | |
| TGAGTAGTCATGACTTGACT+TGG | - | Chr2:25401460-25401479 | None:intergenic | 40.0% | |
| TTTGCGTCATGAATGTCTCT+TGG | - | Chr2:25401228-25401247 | None:intergenic | 40.0% | |
| ATCACCATGGGGAGTTCATA+AGG | + | Chr2:25401147-25401166 | MsG0280008093.01.T01:CDS | 45.0% | |
| CATATTTGGCCATCACCATG+GGG | + | Chr2:25401136-25401155 | MsG0280008093.01.T01:CDS | 45.0% | |
| CTCATATTTGGCCATCACCA+TGG | + | Chr2:25401134-25401153 | MsG0280008093.01.T01:CDS | 45.0% | |
| GATGGCCAAATATGAGGTTG+AGG | - | Chr2:25401130-25401149 | None:intergenic | 45.0% | |
| TCATTGTCAATGACGCTGCT+CGG | + | Chr2:25400999-25401018 | MsG0280008093.01.T01:CDS | 45.0% | |
| TTATGAACTCCCCATGGTGA+TGG | - | Chr2:25401148-25401167 | None:intergenic | 45.0% | |
| !! | CATGACGCAAAGGGTTTTGA+AGG | + | Chr2:25401237-25401256 | MsG0280008093.01.T01:CDS | 45.0% |
| !! | CATGGTGATGGCCAAATATG+AGG | - | Chr2:25401136-25401155 | None:intergenic | 45.0% |
| !! | TTGTTGGTGGTGGTGTCATT+TGG | - | Chr2:25401502-25401521 | None:intergenic | 45.0% |
| !!! | GCAAAGGGTTTTGAAGGCTA+AGG | + | Chr2:25401243-25401262 | MsG0280008093.01.T01:CDS | 45.0% |
| AGCACCTTATGAACTCCCCA+TGG | - | Chr2:25401154-25401173 | None:intergenic | 50.0% | |
| GATGAACTGAGCACGCTTTG+TGG | + | Chr2:25401067-25401086 | MsG0280008093.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 25400971 | 25401528 | 25400971 | ID=MsG0280008093.01;Name=MsG0280008093.01 |
| Chr2 | mRNA | 25400971 | 25401528 | 25400971 | ID=MsG0280008093.01.T01;Parent=MsG0280008093.01;Name=MsG0280008093.01.T01;_AED=0.31;_eAED=0.31;_QI=0|-1|0|1|-1|1|1|0|185 |
| Chr2 | exon | 25400971 | 25401528 | 25400971 | ID=MsG0280008093.01.T01:exon:3258;Parent=MsG0280008093.01.T01 |
| Chr2 | CDS | 25400971 | 25401528 | 25400971 | ID=MsG0280008093.01.T01:cds;Parent=MsG0280008093.01.T01 |
| Gene Sequence |
| Protein sequence |
