AlfalfaGEDB | MsG0280008133.01

M. sativa cultivar ZhongmuNo.1 / MsG0280008133.01

KEGG: -
GO: -
PFAM: PF04640

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008133.01.T01	XP_039686395.1	85.59	229	14	2	4	219	36	258	1.41E-135	390
MsG0280008133.01.T02	XP_039686395.1	99.115	226	2	0	1	226	33	258	8.54E-167	470
MsG0280008133.01.T03	XP_039686395.1	99.07	215	2	0	1	215	44	258	4.32E-157	445
MsG0280008133.01.T04	XP_039686395.1	95.455	132	6	0	7	138	36	167	1.37E-88	269
MsG0280008133.01.T05	XP_039686395.1	95.161	124	6	0	1	124	44	167	3.94E-81	249
MsG0280008133.01.T06	AES65165.2	88.136	118	6	1	1	110	44	161	5.01E-67	213

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008133.01.T01	Q1G3Q4	36.154	260	112	5	4	218	5	255	3.13E-43	149
MsG0280008133.01.T02	Q1G3Q4	40.157	254	117	4	4	225	5	255	1.06E-53	176
MsG0280008133.01.T03	Q1G3Q4	39.344	244	113	4	3	214	15	255	2.27E-48	162
MsG0280008133.01.T04	Q1G3Q4	58.647	133	55	0	3	135	1	133	3.15E-51	166
MsG0280008133.01.T05	Q1G3Q4	59.664	119	48	0	3	121	15	133	1.03E-45	152
MsG0280008133.01.T06	Q1G3Q4	52.101	119	43	1	3	107	15	133	2.19E-34	122

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008133.01.T01	A0A396J5D9	85.590	229	14	2	4	219	36	258	6.74e-136	390
MsG0280008133.01.T02	A0A396J5D9	99.115	226	2	0	1	226	33	258	4.08e-167	470
MsG0280008133.01.T03	A0A396J5D9	99.070	215	2	0	1	215	44	258	2.06e-157	445
MsG0280008133.01.T04	A0A396J5D9	95.455	132	6	0	7	138	36	167	6.56e-89	269
MsG0280008133.01.T05	A0A396J5D9	95.161	124	6	0	1	124	44	167	1.88e-81	249
MsG0280008133.01.T06	G7IG36	88.136	118	6	1	1	110	44	161	2.39e-67	213

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TFs/TRs:

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Gene ID	Type	Classification
MsG0280008133.01.T03	TF	PLATZ
MsG0280008133.01.T05	TF	PLATZ
MsG0280008133.01.T06	TF	PLATZ

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008133.01.T01	MTR_2g036170	86.026	229	7	3	4	219	36	252	7.55e-139	389
MsG0280008133.01.T01	MTR_4g094680	65.333	225	65	3	1	218	18	236	3.79e-99	288
MsG0280008133.01.T01	MTR_4g116300	40.637	251	107	6	4	218	5	249	1.35e-51	167
MsG0280008133.01.T01	MTR_2g055210	39.113	248	113	7	2	218	99	339	1.90e-47	159
MsG0280008133.01.T01	MTR_4g078170	33.929	224	110	8	5	219	15	209	3.99e-31	114
MsG0280008133.01.T01	MTR_5g007230	32.273	220	112	6	5	219	21	208	4.23e-30	111
MsG0280008133.01.T01	MTR_5g030130	30.631	222	128	4	2	219	32	231	3.06e-28	107
MsG0280008133.01.T01	MTR_8g066820	36.691	139	75	3	2	136	30	159	8.28e-26	100
MsG0280008133.01.T01	MTR_5g030130	36.620	142	79	3	2	140	32	165	1.55e-25	98.6
MsG0280008133.01.T01	MTR_1g062160	30.594	219	125	7	5	219	7	202	6.13e-25	97.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008133.01.T01	AT1G31040	55.274	237	74	5	4	219	18	243	9.00e-83	246
MsG0280008133.01.T01	AT3G60670	37.398	246	114	6	5	218	7	244	1.03e-45	152
MsG0280008133.01.T01	AT2G12646	36.398	261	112	5	3	218	1	252	3.88e-45	151
MsG0280008133.01.T01	AT2G12646	36.154	260	112	5	4	218	5	255	3.19e-44	149
MsG0280008133.01.T01	AT2G12646	36.154	260	112	5	4	218	5	255	3.19e-44	149
MsG0280008133.01.T01	AT1G43000	33.484	221	131	6	3	218	7	216	4.67e-29	108
MsG0280008133.01.T01	AT1G43000	33.484	221	131	6	3	218	14	223	4.70e-29	108
MsG0280008133.01.T01	AT2G01818	43.662	142	62	5	6	136	12	146	2.44e-27	104
MsG0280008133.01.T01	AT1G21000	28.627	255	117	6	5	218	16	246	6.29e-27	103
MsG0280008133.01.T01	AT1G21000	28.627	255	117	6	5	218	13	243	6.42e-27	103

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Find 58 sgRNAs with CRISPR-Local

Find 606 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACATTCTTTGGTGATTGT+GGG	0.470651	2:+25859318	MsG0280008133.01.T02:CDS
AAATCCAACAGATTCCTCCC+TGG	0.630961	2:+25870440	MsG0280008133.01.T02:CDS
AACAAAGTGAGATTGTTTAT+TGG	0.287318	2:+25863845	MsG0280008133.01.T02:intron
AACATTCTTTGGTGATTGTG+GGG	0.611739	2:+25859319	MsG0280008133.01.T02:CDS
AAGAAAAGGTGCTCCTCAAA+GGG	0.524805	2:+25870487	MsG0280008133.01.T02:CDS
AAGCATGAGAAGATAAGCAG+TGG	0.608759	2:-25859409	None:intergenic
AATAATGGTTTAGGATAGTG+GGG	0.654932	2:-25870510	None:intergenic
AATGCCAGGGAGGAATCTGT+TGG	0.577735	2:-25870444	None:intergenic
ACTAAGAATGGATGGATCTG+AGG	0.607142	2:+25870319	MsG0280008133.01.T02:CDS
ACTTTGCATGACTTTGATTG+TGG	0.529762	2:-25864260	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAAAAATGCAAGCGGAT+AGG	-	Chr2:25865275-25865294	None:intergenic	30.0%
AAAAAGTGAACAATCTTCGT+GGG	+	Chr2:25868115-25868134	MsG0280008133.01.T02:intron	30.0%
AAAATACATCATCAACCCTT+GGG	+	Chr2:25868165-25868184	MsG0280008133.01.T02:intron	30.0%
AAAATCGATCGGTAAAAAGT+CGG	-	Chr2:25862971-25862990	None:intergenic	30.0%
AAACCTTCCATAAATTCAAG+AGG	-	Chr2:25861577-25861596	None:intergenic	30.0%
AACACTCTTCACTATCATAT+TGG	+	Chr2:25869687-25869706	MsG0280008133.01.T02:intron	30.0%
AACATGAATTGGGAATCAAT+GGG	-	Chr2:25861470-25861489	None:intergenic	30.0%
AACCTAATAAAGCAAATGAG+CGG	-	Chr2:25865193-25865212	None:intergenic	30.0%
AATAAATACATAAGTGCAGG+TGG	+	Chr2:25870211-25870230	MsG0280008133.01.T02:intron	30.0%
AATCAATTGAAGTTGACAAG+GGG	-	Chr2:25859632-25859651	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	25859269	25870523	25859269	ID=MsG0280008133.01;Name=MsG0280008133.01
Chr2	mRNA	25859278	25870523	25859278	ID=MsG0280008133.01.T01;Parent=MsG0280008133.01;Name=MsG0280008133.01.T01;_AED=0.40;_eAED=0.40;_QI=0\|0\|0\|1\|0\|0\|5\|0\|219
Chr2	exon	25863763	25863897	25863763	ID=MsG0280008133.01.T01:exon:21736;Parent=MsG0280008133.01.T01
Chr2	exon	25864257	25864355	25864257	ID=MsG0280008133.01.T01:exon:21737;Parent=MsG0280008133.01.T01
Chr2	exon	25870230	25870384	25870230	ID=MsG0280008133.01.T01:exon:21738;Parent=MsG0280008133.01.T01
Chr2	exon	25870424	25870523	25870424	ID=MsG0280008133.01.T01:exon:21739;Parent=MsG0280008133.01.T01
Chr2	CDS	25859278	25859448	25859278	ID=MsG0280008133.01.T01:cds;Parent=MsG0280008133.01.T01
Chr2	CDS	25863763	25863897	25863763	ID=MsG0280008133.01.T01:cds;Parent=MsG0280008133.01.T01
Chr2	CDS	25864257	25864355	25864257	ID=MsG0280008133.01.T01:cds;Parent=MsG0280008133.01.T01
Chr2	CDS	25870230	25870384	25870230	ID=MsG0280008133.01.T01:cds;Parent=MsG0280008133.01.T01
Chr2	CDS	25870424	25870523	25870424	ID=MsG0280008133.01.T01:cds;Parent=MsG0280008133.01.T01
Chr2	mRNA	25859278	25870523	25859278	ID=MsG0280008133.01.T02;Parent=MsG0280008133.01;Name=MsG0280008133.01.T02;_AED=0.40;_eAED=0.40;_QI=0\|0\|0\|1\|0\|0\|4\|0\|226
Chr2	CDS	25859278	25859448	25859278	ID=MsG0280008133.01.T02:cds;Parent=MsG0280008133.01.T02
Chr2	CDS	25863763	25863837	25863763	ID=MsG0280008133.01.T02:cds;Parent=MsG0280008133.01.T02
Chr2	CDS	25864215	25864355	25864215	ID=MsG0280008133.01.T02:cds;Parent=MsG0280008133.01.T02
Chr2	CDS	25870230	25870523	25870230	ID=MsG0280008133.01.T02:cds;Parent=MsG0280008133.01.T02
Chr2	mRNA	25859311	25870523	25859311	ID=MsG0280008133.01.T03;Parent=MsG0280008133.01;Name=MsG0280008133.01.T03;_AED=0.41;_eAED=0.41;_QI=0\|0\|0\|1\|0\|0\|4\|0\|215
Chr2	CDS	25859311	25859448	25859311	ID=MsG0280008133.01.T03:cds;Parent=MsG0280008133.01.T03
Chr2	CDS	25863763	25863837	25863763	ID=MsG0280008133.01.T03:cds;Parent=MsG0280008133.01.T03
Chr2	CDS	25864215	25864355	25864215	ID=MsG0280008133.01.T03:cds;Parent=MsG0280008133.01.T03
Chr2	CDS	25870230	25870523	25870230	ID=MsG0280008133.01.T03:cds;Parent=MsG0280008133.01.T03
Chr2	mRNA	25859269	25864409	25859269	ID=MsG0280008133.01.T04;Parent=MsG0280008133.01;Name=MsG0280008133.01.T04;_AED=0.46;_eAED=0.46;_QI=0\|0\|0\|1\|0\|0\|3\|0\|149
Chr2	exon	25859269	25859448	25859269	ID=MsG0280008133.01.T04:exon:21744;Parent=MsG0280008133.01.T04
Chr2	CDS	25859269	25859448	25859269	ID=MsG0280008133.01.T04:cds;Parent=MsG0280008133.01.T04
Chr2	CDS	25863763	25863837	25863763	ID=MsG0280008133.01.T04:cds;Parent=MsG0280008133.01.T04
Chr2	CDS	25864215	25864409	25864215	ID=MsG0280008133.01.T04:cds;Parent=MsG0280008133.01.T04
Chr2	mRNA	25859311	25864409	25859311	ID=MsG0280008133.01.T05;Parent=MsG0280008133.01;Name=MsG0280008133.01.T05;_AED=0.47;_eAED=0.47;_QI=0\|0\|0\|1\|0\|0\|3\|0\|135
Chr2	CDS	25859311	25859448	25859311	ID=MsG0280008133.01.T05:cds;Parent=MsG0280008133.01.T05
Chr2	CDS	25863763	25863837	25863763	ID=MsG0280008133.01.T05:cds;Parent=MsG0280008133.01.T05
Chr2	CDS	25864215	25864409	25864215	ID=MsG0280008133.01.T05:cds;Parent=MsG0280008133.01.T05
Chr2	mRNA	25859311	25864409	25859311	ID=MsG0280008133.01.T06;Parent=MsG0280008133.01;Name=MsG0280008133.01.T06;_AED=0.48;_eAED=0.48;_QI=0\|0\|0\|1\|0\|0\|3\|0\|121
Chr2	exon	25864257	25864409	25864257	ID=MsG0280008133.01.T06:exon:21746;Parent=MsG0280008133.01.T06
Chr2	CDS	25859311	25859448	25859311	ID=MsG0280008133.01.T06:cds;Parent=MsG0280008133.01.T06
Chr2	CDS	25863763	25863837	25863763	ID=MsG0280008133.01.T06:cds;Parent=MsG0280008133.01.T06
Chr2	CDS	25864257	25864409	25864257	ID=MsG0280008133.01.T06:cds;Parent=MsG0280008133.01.T06

Gene Sequence

>MsG0280008133.01.T04

ATGAGTATGATGAATATGAAACCTGCATGGCTAGAGAGGTTGATGGATGAAACATTCTTTGGTGATTGTGGGGTCCACAAGAATCGAAGGAAGAACGAGAAGAACATTTTTTGCTTACACTGTTGTCTTAGCATCTGCCCCCACTGCTTATCTTCTCATGCTTCTCATCCTCTTCTCCAGGTGAGAAGGTATGTTTACAACAACGTAATTCGATTGGATGATCTGGAAAAGCTCATTGATTGTTCTAATATACAGCCCTATACTATTAACAGTGCAAAAGTGATATTTTTAAATCAGAGGCCACAATCAAAGTCATGCAAAGTTACAACCAATGCTTGCTTCACTTGTGACAGGATTCTTCAGGACCCATTCCATTTCTGTTCTCTCTCATGCAAGGTTTGTTTTCCCATTTTTCTTTCTCCTTTCTTTTTTTGGTCTCAATTTACATAA

>MsG0280008133.01.T02

ATGAATATGAAACCTGCATGGCTAGAGAGGTTGATGGATGAAACATTCTTTGGTGATTGTGGGGTCCACAAGAATCGAAGGAAGAACGAGAAGAACATTTTTTGCTTACACTGTTGTCTTAGCATCTGCCCCCACTGCTTATCTTCTCATGCTTCTCATCCTCTTCTCCAGGTGAGAAGGTATGTTTACAACAACGTAATTCGATTGGATGATCTGGAAAAGCTCATTGATTGTTCTAATATACAGCCCTATACTATTAACAGTGCAAAAGTGATATTTTTAAATCAGAGGCCACAATCAAAGTCATGCAAAGTTACAACCAATGCTTGCTTCACTTGTGACAGGATTCTTCAGGACCCATTCCATTTCTGTTCTCTCTCATGCAAGGTGGATTTCATGGTATACGAGGGTCAAAGTTTGTCCAACATTTTACATCGATTTGATGAATCTGACTTAGGAATATCGCAATTCGAGGGACTAAGAATGGATGGATCTGAGGTAATTGATGAAGATAACAACCACATTGGCCCAAGTTCTTCTAATTACTCCATCACTCACCAAGAAGAGGCCACCAGCAACAAAATCACCAAGAAGAACAAATCCAACAGATTCCTCCCTGGCATTGTCCTCTCTCTTGGAAGCAGAAGAAAAGGTGCTCCTCAAAGGGCCCCACTATCCTAA

>MsG0280008133.01.T01

ATGAATATGAAACCTGCATGGCTAGAGAGGTTGATGGATGAAACATTCTTTGGTGATTGTGGGGTCCACAAGAATCGAAGGAAGAACGAGAAGAACATTTTTTGCTTACACTGTTGTCTTAGCATCTGCCCCCACTGCTTATCTTCTCATGCTTCTCATCCTCTTCTCCAGGTGAGAAGGTATGTTTACAACAACGTAATTCGATTGGATGATCTGGAAAAGCTCATTGATTGTTCTAATATACAGGTTAGACAACAAAGTGAGATTGTTTATTGGTTTATACTTCAAAAATCAAAATTGAGATCAAGGCCACAATCAAAGTCATGCAAAGTTACAACCAATGCTTGCTTCACTTGTGACAGGATTCTTCAGGACCCATTCCATTTCTGTTCTCTCTCATGCAAGGTGGATTTCATGGTATACGAGGGTCAAAGTTTGTCCAACATTTTACATCGATTTGATGAATCTGACTTAGGAATATCGCAATTCGAGGGACTAAGAATGGATGGATCTGAGGTAATTGATGAAGATAACAACCACATTGGCCCAAGTTCTTCTAAAATCACCAAGAAGAACAAATCCAACAGATTCCTCCCTGGCATTGTCCTCTCTCTTGGAAGCAGAAGAAAAGGTGCTCCTCAAAGGGCCCCACTATCCTAA

>MsG0280008133.01.T05

>MsG0280008133.01.T06

>MsG0280008133.01.T03

ATGGATGAAACATTCTTTGGTGATTGTGGGGTCCACAAGAATCGAAGGAAGAACGAGAAGAACATTTTTTGCTTACACTGTTGTCTTAGCATCTGCCCCCACTGCTTATCTTCTCATGCTTCTCATCCTCTTCTCCAGGTGAGAAGGTATGTTTACAACAACGTAATTCGATTGGATGATCTGGAAAAGCTCATTGATTGTTCTAATATACAGCCCTATACTATTAACAGTGCAAAAGTGATATTTTTAAATCAGAGGCCACAATCAAAGTCATGCAAAGTTACAACCAATGCTTGCTTCACTTGTGACAGGATTCTTCAGGACCCATTCCATTTCTGTTCTCTCTCATGCAAGGTGGATTTCATGGTATACGAGGGTCAAAGTTTGTCCAACATTTTACATCGATTTGATGAATCTGACTTAGGAATATCGCAATTCGAGGGACTAAGAATGGATGGATCTGAGGTAATTGATGAAGATAACAACCACATTGGCCCAAGTTCTTCTAATTACTCCATCACTCACCAAGAAGAGGCCACCAGCAACAAAATCACCAAGAAGAACAAATCCAACAGATTCCTCCCTGGCATTGTCCTCTCTCTTGGAAGCAGAAGAAAAGGTGCTCCTCAAAGGGCCCCACTATCCTAA

Protein sequence

>MsG0280008133.01.T04

MSMMNMKPAWLERLMDETFFGDCGVHKNRRKNEKNIFCLHCCLSICPHCLSSHASHPLLQVRRYVYNNVIRLDDLEKLIDCSNIQPYTINSAKVIFLNQRPQSKSCKVTTNACFTCDRILQDPFHFCSLSCKVCFPIFLSPFFFWSQFT*

>MsG0280008133.01.T02

MNMKPAWLERLMDETFFGDCGVHKNRRKNEKNIFCLHCCLSICPHCLSSHASHPLLQVRRYVYNNVIRLDDLEKLIDCSNIQPYTINSAKVIFLNQRPQSKSCKVTTNACFTCDRILQDPFHFCSLSCKVDFMVYEGQSLSNILHRFDESDLGISQFEGLRMDGSEVIDEDNNHIGPSSSNYSITHQEEATSNKITKKNKSNRFLPGIVLSLGSRRKGAPQRAPLS*

>MsG0280008133.01.T01

MNMKPAWLERLMDETFFGDCGVHKNRRKNEKNIFCLHCCLSICPHCLSSHASHPLLQVRRYVYNNVIRLDDLEKLIDCSNIQVRQQSEIVYWFILQKSKLRSRPQSKSCKVTTNACFTCDRILQDPFHFCSLSCKVDFMVYEGQSLSNILHRFDESDLGISQFEGLRMDGSEVIDEDNNHIGPSSSKITKKNKSNRFLPGIVLSLGSRRKGAPQRAPLS*

>MsG0280008133.01.T05

MDETFFGDCGVHKNRRKNEKNIFCLHCCLSICPHCLSSHASHPLLQVRRYVYNNVIRLDDLEKLIDCSNIQPYTINSAKVIFLNQRPQSKSCKVTTNACFTCDRILQDPFHFCSLSCKVCFPIFLSPFFFWSQFT*

>MsG0280008133.01.T06

MDETFFGDCGVHKNRRKNEKNIFCLHCCLSICPHCLSSHASHPLLQVRRYVYNNVIRLDDLEKLIDCSNIQRPQSKSCKVTTNACFTCDRILQDPFHFCSLSCKVCFPIFLSPFFFWSQFT*

>MsG0280008133.01.T03

MDETFFGDCGVHKNRRKNEKNIFCLHCCLSICPHCLSSHASHPLLQVRRYVYNNVIRLDDLEKLIDCSNIQPYTINSAKVIFLNQRPQSKSCKVTTNACFTCDRILQDPFHFCSLSCKVDFMVYEGQSLSNILHRFDESDLGISQFEGLRMDGSEVIDEDNNHIGPSSSNYSITHQEEATSNKITKKNKSNRFLPGIVLSLGSRRKGAPQRAPLS*