AlfalfaGEDB | MsG0280008594.01

M. sativa cultivar ZhongmuNo.1 / MsG0280008594.01

KEGG: -
GO: GO:0003677 GO:0046983
PFAM: PF00319

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008594.01.T01	RHN73765.1	75.556	180	32	3	1	174	16	189	6.53E-82	251
MsG0280008594.01.T02	RHN73765.1	79.487	156	24	2	1	155	41	189	6.35E-76	235

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008594.01.T01	Q9FKK2	32.278	158	91	7	17	165	10	160	3.09E-12	66.6
MsG0280008594.01.T02	Q4PSU4	28.378	148	102	4	1	146	69	214	1.76E-12	66.2

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008594.01.T01	A0A396JBD2	75.556	180	32	3	1	174	16	189	3.12e-82	251
MsG0280008594.01.T02	A0A396JBD2	79.487	156	24	2	1	155	41	189	3.03e-76	235

TFs/TRs:

Gene ID	Type	Classification
MsG0280008594.01.T02	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008594.01.T01	MTR_2g045020	75.556	180	32	3	1	174	1	174	1.37e-85	249
MsG0280008594.01.T01	MTR_3g437790	63.889	144	49	3	34	175	32	174	1.15e-57	179
MsG0280008594.01.T01	MTR_8g022970	50.920	163	73	5	17	175	18	177	1.60e-42	140
MsG0280008594.01.T01	MTR_3g109930	50.633	158	73	4	19	175	7	160	2.10e-41	137
MsG0280008594.01.T01	MTR_7g028448	33.824	136	76	3	34	168	31	153	2.28e-17	75.5
MsG0280008594.01.T01	MTR_4g036915	33.824	136	76	3	34	168	31	153	2.28e-17	75.5
MsG0280008594.01.T01	MTR_2g105290	35.220	159	95	5	18	173	13	166	4.33e-17	75.1
MsG0280008594.01.T01	MTR_8g086290	35.294	170	98	7	7	173	6	166	1.37e-15	70.9
MsG0280008594.01.T01	MTR_8g043650	33.939	165	88	7	8	162	3	156	5.66e-14	67.8
MsG0280008594.01.T01	MTR_5g053390	32.298	161	102	6	8	165	3	159	2.15e-13	66.2
MsG0280008594.01.T01	MTR_2g030740	35.220	159	82	7	17	165	12	159	4.04e-13	65.5
MsG0280008594.01.T01	MTR_3g052920	32.857	140	78	4	34	165	28	159	1.68e-12	63.9
MsG0280008594.01.T01	MTR_1g105910	35.252	139	70	6	34	162	28	156	2.55e-12	63.2
MsG0280008594.01.T01	MTR_1g106070	34.507	142	73	6	34	165	28	159	3.41e-12	62.0
MsG0280008594.01.T01	MTR_1g012570	32.857	140	78	6	34	165	28	159	6.15e-12	62.4
MsG0280008594.01.T01	MTR_3g080940	28.395	162	95	7	17	168	12	162	2.32e-11	60.1
MsG0280008594.01.T01	MTR_3g030770	33.571	140	77	5	34	165	28	159	3.50e-11	60.1
MsG0280008594.01.T01	MTR_5g045560	29.878	164	96	7	8	162	3	156	3.79e-11	60.1
MsG0280008594.01.T01	MTR_1g105920	33.803	142	74	6	34	165	28	159	8.12e-11	59.3
MsG0280008594.01.T01	MTR_1g108510	33.571	140	77	5	34	165	28	159	8.39e-11	58.9
MsG0280008594.01.T02	MTR_2g045020	78.710	155	27	1	1	155	26	174	1.29e-79	234
MsG0280008594.01.T02	MTR_3g437790	63.889	144	49	3	15	156	32	174	1.39e-57	178
MsG0280008594.01.T02	MTR_8g022970	51.266	158	70	5	3	156	23	177	8.57e-42	138
MsG0280008594.01.T02	MTR_3g109930	53.147	143	63	3	15	156	21	160	1.80e-41	137
MsG0280008594.01.T02	MTR_7g028448	33.824	136	76	3	15	149	31	153	1.40e-17	75.5
MsG0280008594.01.T02	MTR_4g036915	33.824	136	76	3	15	149	31	153	1.40e-17	75.5
MsG0280008594.01.T02	MTR_2g105290	35.484	155	92	5	3	154	17	166	4.60e-17	74.3
MsG0280008594.01.T02	MTR_8g086290	34.194	155	94	5	3	154	17	166	2.00e-14	67.4
MsG0280008594.01.T02	MTR_8g043650	35.915	142	71	6	15	146	28	159	1.41e-13	66.2
MsG0280008594.01.T02	MTR_2g030740	35.256	156	80	7	1	146	15	159	3.61e-13	65.1
MsG0280008594.01.T02	MTR_5g053390	33.333	135	84	5	15	146	28	159	8.94e-13	63.9
MsG0280008594.01.T02	MTR_3g052920	32.857	140	78	4	15	146	28	159	1.16e-12	63.5
MsG0280008594.01.T02	MTR_1g105910	35.211	142	72	6	15	146	28	159	1.70e-12	63.2
MsG0280008594.01.T02	MTR_1g106070	34.507	142	73	6	15	146	28	159	2.77e-12	61.6
MsG0280008594.01.T02	MTR_1g012570	32.857	140	78	6	15	146	28	159	5.86e-12	61.6
MsG0280008594.01.T02	MTR_5g055100	31.884	138	76	5	15	143	28	156	6.76e-12	61.6
MsG0280008594.01.T02	MTR_3g080940	28.966	145	83	6	15	149	28	162	1.68e-11	60.1
MsG0280008594.01.T02	MTR_3g030770	33.571	140	77	5	15	146	28	159	1.97e-11	60.1
MsG0280008594.01.T02	MTR_1g108510	33.571	140	77	5	15	146	28	159	4.87e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008594.01.T01	AT2G24840	28.477	151	104	4	17	165	66	214	2.98e-13	66.2
MsG0280008594.01.T01	AT5G60440	32.278	158	91	7	17	165	10	160	3.15e-13	66.6
MsG0280008594.01.T02	AT2G24840	28.378	148	102	4	1	146	69	214	1.80e-13	66.2
MsG0280008594.01.T02	AT5G60440	32.258	155	89	7	1	146	13	160	2.47e-13	66.2

Find 20 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAAGACTTTAAGAATTCTT+TGG	0.239126	2:+34500136	MsG0280008594.01.T01:CDS
GTGCTTCATATTGAAGCCTT+AGG	0.338858	2:-34499986	None:intergenic
GTGATCTCAATTTCCATGAT+TGG	0.375502	2:+34500075	MsG0280008594.01.T01:CDS
TAGAATTATTCCACCAATCA+TGG	0.397833	2:-34500088	None:intergenic
GCATAGAACTTGTTCTTGTC+TGG	0.418752	2:-34499861	None:intergenic
TGCTTCATATTGAAGCCTTA+GGG	0.444644	2:-34499985	None:intergenic
ACGATGAATGACAGCATCAC+AGG	0.499770	2:-34499895	None:intergenic
GACATATGATGGTCGACGAT+GGG	0.514442	2:+34499928	MsG0280008594.01.T01:CDS
ATGAAATTGCTGAAACCCTA+AGG	0.533334	2:+34499970	MsG0280008594.01.T01:CDS
ATCGTCGACCATCATATGTC+CGG	0.542227	2:-34499925	None:intergenic
ATCTCAATTTCCATGATTGG+TGG	0.554115	2:+34500078	MsG0280008594.01.T01:CDS
GAGTTCTCAAGACGCAAACT+CGG	0.557665	2:+34499771	MsG0280008594.01.T01:CDS
TGTCTGAAAAGAACACTGCT+AGG	0.558447	2:+34499655	MsG0280008594.01.T01:CDS
AATTTACGTGCAGCAAGCGA+TGG	0.560913	2:+34500173	MsG0280008594.01.T01:CDS
GGACATATGATGGTCGACGA+TGG	0.562958	2:+34499927	MsG0280008594.01.T01:CDS
TCTTGTCTGGCGATTTGACG+AGG	0.587599	2:-34499848	None:intergenic
CGATGAATGACAGCATCACA+GGG	0.591570	2:-34499894	None:intergenic
GTCTGAAAAGAACACTGCTA+GGG	0.626438	2:+34499656	MsG0280008594.01.T01:CDS
AAGAACAAGTTCTATGCATG+TGG	0.630985	2:+34499867	MsG0280008594.01.T01:CDS
GATGAATGACAGCATCACAG+GGG	0.800398	2:-34499893	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAGAAAGAAGAAAAACTGAA+CGG	+	Chr2:34499744-34499763	MsG0280008594.01.T01:CDS	25.0%
!	AATTCTATGCCAATCAAAAA+CGG	+	Chr2:34499821-34499840	MsG0280008594.01.T01:CDS	25.0%
!!	TGAAGACTTTAAGAATTCTT+TGG	+	Chr2:34500136-34500155	MsG0280008594.01.T01:CDS	25.0%
!!!	AATTCTTTGGAGAGTTTTAA+GGG	+	Chr2:34500149-34500168	MsG0280008594.01.T01:CDS	25.0%
	TAGAATTATTCCACCAATCA+TGG	-	Chr2:34500091-34500110	None:intergenic	30.0%
	TCAAGAAAAACTTGAAGAAG+AGG	+	Chr2:34500010-34500029	MsG0280008594.01.T01:CDS	30.0%
!!	GAATTCTTTGGAGAGTTTTA+AGG	+	Chr2:34500148-34500167	MsG0280008594.01.T01:CDS	30.0%
	AAGAACAAGTTCTATGCATG+TGG	+	Chr2:34499867-34499886	MsG0280008594.01.T01:CDS	35.0%
	ATCTCAATTTCCATGATTGG+TGG	+	Chr2:34500078-34500097	MsG0280008594.01.T01:CDS	35.0%
	ATGAAATTGCTGAAACCCTA+AGG	+	Chr2:34499970-34499989	MsG0280008594.01.T01:CDS	35.0%
	GAACAAAACTGAACAACACA+CGG	+	Chr2:34499700-34499719	MsG0280008594.01.T01:intron	35.0%
	GTGATCTCAATTTCCATGAT+TGG	+	Chr2:34500075-34500094	MsG0280008594.01.T01:CDS	35.0%
	TGCTTCATATTGAAGCCTTA+GGG	-	Chr2:34499988-34500007	None:intergenic	35.0%
	GTCTGAAAAGAACACTGCTA+GGG	+	Chr2:34499656-34499675	MsG0280008594.01.T01:CDS	40.0%
	GTGCTTCATATTGAAGCCTT+AGG	-	Chr2:34499989-34500008	None:intergenic	40.0%
	TGTCTGAAAAGAACACTGCT+AGG	+	Chr2:34499655-34499674	MsG0280008594.01.T01:CDS	40.0%
	TGTTCCAGCATTCTCTCTTT+CGG	-	Chr2:34499685-34499704	None:intergenic	40.0%
!	GAGATCACTCTTTTGTGCTT+CGG	-	Chr2:34500063-34500082	None:intergenic	40.0%
!	GCATAGAACTTGTTCTTGTC+TGG	-	Chr2:34499864-34499883	None:intergenic	40.0%
!	GTCATTCATCGTTTTCTGAC+CGG	+	Chr2:34499906-34499925	MsG0280008594.01.T01:CDS	40.0%
!	TTTTCTGACCGGACATATGA+TGG	+	Chr2:34499917-34499936	MsG0280008594.01.T01:CDS	40.0%
	AATTTACGTGCAGCAAGCGA+TGG	+	Chr2:34500173-34500192	MsG0280008594.01.T01:CDS	45.0%
	ACGATGAATGACAGCATCAC+AGG	-	Chr2:34499898-34499917	None:intergenic	45.0%
	ATCGTCGACCATCATATGTC+CGG	-	Chr2:34499928-34499947	None:intergenic	45.0%
	CGATGAATGACAGCATCACA+GGG	-	Chr2:34499897-34499916	None:intergenic	45.0%
	GACATATGATGGTCGACGAT+GGG	+	Chr2:34499928-34499947	MsG0280008594.01.T01:CDS	45.0%
	GAGTTCTCAAGACGCAAACT+CGG	+	Chr2:34499771-34499790	MsG0280008594.01.T01:CDS	45.0%
	GATGAATGACAGCATCACAG+GGG	-	Chr2:34499896-34499915	None:intergenic	45.0%
	GCTTCCGAAAGAGAGAATGC+TGG	+	Chr2:34499678-34499697	MsG0280008594.01.T01:intron	50.0%
	GGACATATGATGGTCGACGA+TGG	+	Chr2:34499927-34499946	MsG0280008594.01.T01:CDS	50.0%
	TCTTGTCTGGCGATTTGACG+AGG	-	Chr2:34499851-34499870	None:intergenic	50.0%
!!!	CGAGGAGTGCCGTTTTTGAT+TGG	-	Chr2:34499833-34499852	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	34499636	34500202	34499636	ID=MsG0280008594.01;Name=MsG0280008594.01
Chr2	mRNA	34499636	34500202	34499636	ID=MsG0280008594.01.T01;Parent=MsG0280008594.01;Name=MsG0280008594.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|0\|0\|1\|0\|0\|2\|0\|175
Chr2	exon	34499636	34499683	34499636	ID=MsG0280008594.01.T01:exon:7549;Parent=MsG0280008594.01.T01
Chr2	exon	34499723	34500202	34499723	ID=MsG0280008594.01.T01:exon:7550;Parent=MsG0280008594.01.T01
Chr2	CDS	34499636	34499683	34499636	ID=MsG0280008594.01.T01:cds;Parent=MsG0280008594.01.T01
Chr2	CDS	34499723	34500202	34499723	ID=MsG0280008594.01.T01:cds;Parent=MsG0280008594.01.T01
Chr2	mRNA	34499732	34500202	34499732	ID=MsG0280008594.01.T02;Parent=MsG0280008594.01;Name=MsG0280008594.01.T02;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|156
Chr2	exon	34499732	34500202	34499732	ID=MsG0280008594.01.T02:exon:7551;Parent=MsG0280008594.01.T02
Chr2	CDS	34499732	34500202	34499732	ID=MsG0280008594.01.T02:cds;Parent=MsG0280008594.01.T02

Gene Sequence

>MsG0280008594.01.T01

ATGAATAAGAATTGTGAACTGTCTGAAAAGAACACTGCTAGGGCTTCCAAGAGAAAGATGCATGAAATCAAGAAAGAAGAAAAACTGAACGGTTCTGAGTTCTCAAGACGCAAACTCGGAATATTCAACAAAGCAATAGAGCTGTCAATTCTATGCCAATCAAAAACGGCACTCCTCGTCAAATCGCCAGACAAGAACAAGTTCTATGCATGTGGCTACCCCTGTGATGCTGTCATTCATCGTTTTCTGACCGGACATATGATGGTCGACGATGGGAAAAAGAAGAAACAAGATGATGAAATTGCTGAAACCCTAAGGCTTCAATATGAAGCACTTCAAGAAAAACTTGAAGAAGAGGATGATAATTTGAAAGCTGTCAAAGTCACCGAAGCACAAAAGAGTGATCTCAATTTCCATGATTGGTGGAATAATTCTATTGATGATATGAATTTAGCATCTCTTGAAGACTTTAAGAATTCTTTGGAGAGTTTTAAGGGTAATTTACGTGCAGCAAGCGATGGAAAGTAG

>MsG0280008594.01.T02

ATGCATGAAATCAAGAAAGAAGAAAAACTGAACGGTTCTGAGTTCTCAAGACGCAAACTCGGAATATTCAACAAAGCAATAGAGCTGTCAATTCTATGCCAATCAAAAACGGCACTCCTCGTCAAATCGCCAGACAAGAACAAGTTCTATGCATGTGGCTACCCCTGTGATGCTGTCATTCATCGTTTTCTGACCGGACATATGATGGTCGACGATGGGAAAAAGAAGAAACAAGATGATGAAATTGCTGAAACCCTAAGGCTTCAATATGAAGCACTTCAAGAAAAACTTGAAGAAGAGGATGATAATTTGAAAGCTGTCAAAGTCACCGAAGCACAAAAGAGTGATCTCAATTTCCATGATTGGTGGAATAATTCTATTGATGATATGAATTTAGCATCTCTTGAAGACTTTAAGAATTCTTTGGAGAGTTTTAAGGGTAATTTACGTGCAGCAAGCGATGGAAAGTAG

Protein sequence

>MsG0280008594.01.T01

MNKNCELSEKNTARASKRKMHEIKKEEKLNGSEFSRRKLGIFNKAIELSILCQSKTALLVKSPDKNKFYACGYPCDAVIHRFLTGHMMVDDGKKKKQDDEIAETLRLQYEALQEKLEEEDDNLKAVKVTEAQKSDLNFHDWWNNSIDDMNLASLEDFKNSLESFKGNLRAASDGK*

>MsG0280008594.01.T02

MHEIKKEEKLNGSEFSRRKLGIFNKAIELSILCQSKTALLVKSPDKNKFYACGYPCDAVIHRFLTGHMMVDDGKKKKQDDEIAETLRLQYEALQEKLEEEDDNLKAVKVTEAQKSDLNFHDWWNNSIDDMNLASLEDFKNSLESFKGNLRAASDGK*