Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009219.01.T01 | KEH41323.1 | 98.78 | 82 | 1 | 0 | 10 | 91 | 11 | 92 | 7.97E-53 | 170 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009219.01.T01 | Q7XC57 | 53.623 | 69 | 32 | 0 | 12 | 80 | 95 | 163 | 6.80E-20 | 84.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009219.01.T01 | A0A072VIR9 | 98.780 | 82 | 1 | 0 | 10 | 91 | 11 | 92 | 3.81e-53 | 170 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0280009219.01.T01 | TF | MYB-related |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009219.01.T01 | MTR_1g048660 | 98.780 | 82 | 1 | 0 | 10 | 91 | 11 | 92 | 9.65e-57 | 170 |
| MsG0280009219.01.T01 | MTR_8g101650 | 54.321 | 81 | 35 | 2 | 12 | 91 | 106 | 185 | 7.91e-22 | 86.3 |
| MsG0280009219.01.T01 | MTR_5g088010 | 56.944 | 72 | 28 | 1 | 13 | 81 | 62 | 133 | 1.57e-20 | 82.4 |
| MsG0280009219.01.T01 | MTR_2g100930 | 54.688 | 64 | 29 | 0 | 12 | 75 | 110 | 173 | 4.33e-20 | 82.4 |
| MsG0280009219.01.T01 | MTR_3g104370 | 71.739 | 46 | 13 | 0 | 12 | 57 | 92 | 137 | 2.25e-19 | 79.0 |
| MsG0280009219.01.T01 | MTR_6g092540 | 48.315 | 89 | 36 | 3 | 12 | 91 | 98 | 185 | 5.60e-19 | 79.0 |
| MsG0280009219.01.T01 | MTR_7g067080 | 46.835 | 79 | 42 | 0 | 12 | 90 | 141 | 219 | 6.42e-19 | 79.0 |
| MsG0280009219.01.T01 | MTR_5g088060 | 56.944 | 72 | 28 | 1 | 13 | 81 | 274 | 345 | 1.00e-18 | 79.3 |
| MsG0280009219.01.T01 | MTR_5g488160 | 56.944 | 72 | 28 | 1 | 13 | 81 | 274 | 345 | 1.00e-18 | 79.3 |
| MsG0280009219.01.T01 | MTR_4g100630 | 50.000 | 64 | 32 | 0 | 12 | 75 | 96 | 159 | 1.30e-18 | 78.2 |
| MsG0280009219.01.T01 | MTR_5g069710 | 54.688 | 64 | 29 | 0 | 12 | 75 | 133 | 196 | 1.39e-18 | 78.2 |
| MsG0280009219.01.T01 | MTR_3g462790 | 54.688 | 64 | 29 | 0 | 12 | 75 | 148 | 211 | 1.60e-18 | 78.2 |
| MsG0280009219.01.T01 | MTR_5g027570 | 51.429 | 70 | 32 | 1 | 13 | 80 | 94 | 163 | 2.86e-18 | 76.3 |
| MsG0280009219.01.T01 | MTR_5g037080 | 56.250 | 64 | 28 | 0 | 12 | 75 | 103 | 166 | 6.98e-18 | 76.3 |
| MsG0280009219.01.T01 | MTR_4g111975 | 50.000 | 64 | 32 | 0 | 12 | 75 | 139 | 202 | 2.40e-17 | 74.7 |
| MsG0280009219.01.T01 | MTR_1g111830 | 51.562 | 64 | 31 | 0 | 12 | 75 | 125 | 188 | 3.35e-17 | 73.6 |
| MsG0280009219.01.T01 | MTR_5g081860 | 53.030 | 66 | 29 | 1 | 12 | 75 | 126 | 191 | 5.15e-17 | 73.6 |
| MsG0280009219.01.T01 | MTR_2g090305 | 50.000 | 64 | 32 | 0 | 12 | 75 | 29 | 92 | 1.05e-16 | 71.2 |
| MsG0280009219.01.T01 | MTR_0036s0260 | 50.000 | 64 | 32 | 0 | 12 | 75 | 29 | 92 | 2.96e-16 | 70.5 |
| MsG0280009219.01.T01 | MTR_4g107230 | 54.688 | 64 | 29 | 0 | 12 | 75 | 85 | 148 | 3.18e-16 | 71.6 |
| MsG0280009219.01.T01 | MTR_5g027550 | 64.444 | 45 | 16 | 0 | 13 | 57 | 79 | 123 | 8.04e-16 | 69.7 |
| MsG0280009219.01.T01 | MTR_8g063870 | 49.333 | 75 | 36 | 1 | 13 | 85 | 94 | 168 | 1.51e-15 | 69.3 |
| MsG0280009219.01.T01 | MTR_4g015130 | 50.000 | 66 | 30 | 1 | 21 | 83 | 3 | 68 | 1.66e-14 | 63.2 |
| MsG0280009219.01.T01 | MTR_8g063600 | 45.455 | 77 | 38 | 2 | 12 | 85 | 107 | 182 | 1.94e-14 | 66.6 |
| MsG0280009219.01.T01 | MTR_3g070110 | 65.909 | 44 | 15 | 0 | 13 | 56 | 97 | 140 | 4.20e-13 | 62.4 |
| MsG0280009219.01.T01 | MTR_5g075760 | 58.824 | 51 | 20 | 1 | 7 | 57 | 101 | 150 | 1.38e-12 | 60.8 |
| MsG0280009219.01.T01 | MTR_6g043480 | 46.429 | 56 | 30 | 0 | 9 | 64 | 105 | 160 | 2.28e-12 | 60.5 |
| MsG0280009219.01.T01 | MTR_6g043490 | 44.643 | 56 | 31 | 0 | 9 | 64 | 105 | 160 | 3.52e-12 | 60.1 |
| MsG0280009219.01.T01 | MTR_5g075790 | 54.902 | 51 | 22 | 1 | 7 | 57 | 109 | 158 | 4.55e-11 | 57.0 |
| MsG0280009219.01.T01 | MTR_1g033620 | 53.191 | 47 | 22 | 0 | 29 | 75 | 23 | 69 | 5.48e-11 | 57.0 |
| MsG0280009219.01.T01 | MTR_8g072990 | 43.396 | 53 | 30 | 0 | 12 | 64 | 101 | 153 | 8.80e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009219.01.T01 | AT5G56840 | 55.128 | 78 | 30 | 2 | 12 | 84 | 92 | 169 | 1.87e-21 | 84.7 |
| MsG0280009219.01.T01 | AT5G47390 | 52.941 | 68 | 32 | 0 | 12 | 79 | 97 | 164 | 3.76e-20 | 82.8 |
| MsG0280009219.01.T01 | AT5G58900 | 51.807 | 83 | 36 | 2 | 12 | 91 | 142 | 223 | 8.49e-20 | 81.3 |
| MsG0280009219.01.T01 | AT5G61620 | 51.351 | 74 | 36 | 0 | 11 | 84 | 108 | 181 | 8.76e-20 | 81.6 |
| MsG0280009219.01.T01 | AT1G49010 | 45.455 | 77 | 42 | 0 | 12 | 88 | 135 | 211 | 2.00e-19 | 80.5 |
| MsG0280009219.01.T01 | AT3G11280 | 53.125 | 64 | 30 | 0 | 12 | 75 | 128 | 191 | 3.02e-18 | 76.6 |
| MsG0280009219.01.T01 | AT3G11280 | 53.125 | 64 | 30 | 0 | 12 | 75 | 128 | 191 | 3.02e-18 | 76.6 |
| MsG0280009219.01.T01 | AT2G38090 | 53.125 | 64 | 30 | 0 | 12 | 75 | 142 | 205 | 3.41e-18 | 77.0 |
| MsG0280009219.01.T01 | AT5G04760 | 53.125 | 64 | 30 | 0 | 12 | 75 | 100 | 163 | 4.08e-18 | 75.5 |
| MsG0280009219.01.T01 | AT5G01200 | 54.688 | 64 | 29 | 0 | 12 | 75 | 147 | 210 | 5.35e-18 | 75.9 |
| MsG0280009219.01.T01 | AT1G70000 | 56.250 | 64 | 28 | 0 | 12 | 75 | 99 | 162 | 1.06e-17 | 75.1 |
| MsG0280009219.01.T01 | AT1G70000 | 56.250 | 64 | 28 | 0 | 12 | 75 | 99 | 162 | 1.06e-17 | 75.1 |
| MsG0280009219.01.T01 | AT5G05790 | 45.570 | 79 | 43 | 0 | 12 | 90 | 132 | 210 | 1.38e-17 | 75.1 |
| MsG0280009219.01.T01 | AT5G05790 | 45.570 | 79 | 43 | 0 | 12 | 90 | 132 | 210 | 1.38e-17 | 75.1 |
| MsG0280009219.01.T01 | AT5G08520 | 52.381 | 63 | 30 | 0 | 13 | 75 | 120 | 182 | 2.24e-17 | 74.7 |
| MsG0280009219.01.T01 | AT1G19000 | 55.224 | 67 | 30 | 0 | 12 | 78 | 103 | 169 | 3.40e-17 | 73.9 |
| MsG0280009219.01.T01 | AT1G19000 | 55.224 | 67 | 30 | 0 | 12 | 78 | 103 | 169 | 3.40e-17 | 73.9 |
| MsG0280009219.01.T01 | AT1G74840 | 57.812 | 64 | 27 | 0 | 12 | 75 | 97 | 160 | 9.06e-17 | 72.4 |
| MsG0280009219.01.T01 | AT1G74840 | 57.812 | 64 | 27 | 0 | 12 | 75 | 97 | 160 | 1.17e-16 | 72.4 |
| MsG0280009219.01.T01 | AT3G16350 | 51.471 | 68 | 33 | 0 | 12 | 79 | 138 | 205 | 2.01e-16 | 72.8 |
| MsG0280009219.01.T01 | AT3G10580 | 47.887 | 71 | 35 | 1 | 12 | 82 | 96 | 164 | 4.72e-16 | 71.2 |
| MsG0280009219.01.T01 | AT5G23650 | 49.275 | 69 | 35 | 0 | 12 | 80 | 121 | 189 | 5.69e-16 | 71.2 |
| MsG0280009219.01.T01 | AT3G10580 | 47.887 | 71 | 35 | 1 | 12 | 82 | 96 | 164 | 2.44e-15 | 68.9 |
| MsG0280009219.01.T01 | AT3G10590 | 47.436 | 78 | 36 | 2 | 12 | 87 | 112 | 186 | 3.48e-15 | 67.8 |
| MsG0280009219.01.T01 | AT4G09450 | 48.485 | 66 | 34 | 0 | 12 | 77 | 92 | 157 | 5.97e-15 | 67.0 |
Find 17 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCTTTCTTGAAAGGTTTAA+AGG | 0.205247 | 2:-48052870 | MsG0280009219.01.T01:CDS |
| AGCAACCTGAGTAGGAGTTT+TGG | 0.403614 | 2:+48052799 | None:intergenic |
| GGTTTAAAGGCTGTTGGAAA+AGG | 0.435483 | 2:-48052857 | MsG0280009219.01.T01:CDS |
| TTGAAAGGTTTAAAGGCTGT+TGG | 0.453203 | 2:-48052863 | MsG0280009219.01.T01:CDS |
| TGGTGCTCCGTCCTTGTCCA+TGG | 0.462848 | 2:+48052893 | None:intergenic |
| TTAAACCTTTCAAGAAAGCC+TGG | 0.468661 | 2:+48052873 | None:intergenic |
| GAGCACCAGGCTTTCTTGAA+AGG | 0.474105 | 2:-48052878 | MsG0280009219.01.T01:CDS |
| ATGGACAAGGACGGAGCACC+AGG | 0.487460 | 2:-48052891 | MsG0280009219.01.T01:CDS |
| AATGAAAATTCAAAAGCTAC+TGG | 0.495544 | 2:-48052521 | MsG0280009219.01.T01:CDS |
| AGGCTGTTGGAAAAGGCAAA+TGG | 0.496236 | 2:-48052850 | MsG0280009219.01.T01:CDS |
| GGTGACCAAAACTCCTACTC+AGG | 0.558451 | 2:-48052804 | MsG0280009219.01.T01:CDS |
| CTGGTGCTTCTTCTGTATAG+AGG | 0.565004 | 2:-48052502 | None:intergenic |
| CAAATGGAAGCGAATATCGA+AGG | 0.631038 | 2:-48052834 | MsG0280009219.01.T01:CDS |
| GTTGCATTGTAGTGAAACCA+TGG | 0.639856 | 2:-48052910 | MsG0280009219.01.T01:CDS |
| GCATGACTAGCAACCTGAGT+AGG | 0.641227 | 2:+48052791 | None:intergenic |
| AGTGAAACCATGGACAAGGA+CGG | 0.664978 | 2:-48052900 | MsG0280009219.01.T01:CDS |
| TTGTAGTGAAACCATGGACA+AGG | 0.674968 | 2:-48052904 | MsG0280009219.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATGAAAATTCAAAAGCTAC+TGG | - | Chr2:48052910-48052929 | MsG0280009219.01.T01:CDS | 25.0% |
| ! | AATGACTATATTGCTTGATA+TGG | - | Chr2:48052878-48052897 | MsG0280009219.01.T01:CDS | 25.0% |
| ! | ACATAATGTACGTATAACAA+AGG | + | Chr2:48052851-48052870 | None:intergenic | 25.0% |
| ! | TTTGTACTAAAATTCAACTG+CGG | + | Chr2:48052821-48052840 | None:intergenic | 25.0% |
| !!! | AAGCATTTTAATTATAGACG+TGG | - | Chr2:48052667-48052686 | MsG0280009219.01.T01:intron | 25.0% |
| !!! | CTGATTGAGATTTTTGTTAT+AGG | - | Chr2:48052764-48052783 | MsG0280009219.01.T01:CDS | 25.0% |
| AAATCTCAATCAGAAAGAGA+AGG | + | Chr2:48052757-48052776 | None:intergenic | 30.0% | |
| ACTATATTGCTTGATATGGC+AGG | - | Chr2:48052882-48052901 | MsG0280009219.01.T01:CDS | 35.0% | |
| GGCTTTCTTGAAAGGTTTAA+AGG | - | Chr2:48052561-48052580 | MsG0280009219.01.T01:intron | 35.0% | |
| TTAAACCTTTCAAGAAAGCC+TGG | + | Chr2:48052561-48052580 | None:intergenic | 35.0% | |
| TTGAAAGGTTTAAAGGCTGT+TGG | - | Chr2:48052568-48052587 | MsG0280009219.01.T01:intron | 35.0% | |
| AAATTCAACTGCGGCCTATT+TGG | + | Chr2:48052812-48052831 | None:intergenic | 40.0% | |
| CAAATGGAAGCGAATATCGA+AGG | - | Chr2:48052597-48052616 | MsG0280009219.01.T01:intron | 40.0% | |
| GGTTTAAAGGCTGTTGGAAA+AGG | - | Chr2:48052574-48052593 | MsG0280009219.01.T01:intron | 40.0% | |
| GTTGCATTGTAGTGAAACCA+TGG | - | Chr2:48052521-48052540 | MsG0280009219.01.T01:CDS | 40.0% | |
| TTGTAGTGAAACCATGGACA+AGG | - | Chr2:48052527-48052546 | MsG0280009219.01.T01:CDS | 40.0% | |
| ! | GCGAATATCGAAGGATTTTG+TGG | - | Chr2:48052606-48052625 | MsG0280009219.01.T01:intron | 40.0% |
| AGGCTGTTGGAAAAGGCAAA+TGG | - | Chr2:48052581-48052600 | MsG0280009219.01.T01:intron | 45.0% | |
| AGTGAAACCATGGACAAGGA+CGG | - | Chr2:48052531-48052550 | MsG0280009219.01.T01:intron | 45.0% | |
| TGTGTCTAGTCCCTCCAAAT+AGG | - | Chr2:48052795-48052814 | MsG0280009219.01.T01:CDS | 45.0% | |
| ! | AGCAACCTGAGTAGGAGTTT+TGG | + | Chr2:48052635-48052654 | None:intergenic | 45.0% |
| GAGCACCAGGCTTTCTTGAA+AGG | - | Chr2:48052553-48052572 | MsG0280009219.01.T01:intron | 50.0% | |
| GCATGACTAGCAACCTGAGT+AGG | + | Chr2:48052643-48052662 | None:intergenic | 50.0% | |
| GGTGACCAAAACTCCTACTC+AGG | - | Chr2:48052627-48052646 | MsG0280009219.01.T01:intron | 50.0% | |
| TCAACTGCGGCCTATTTGGA+GGG | + | Chr2:48052808-48052827 | None:intergenic | 50.0% | |
| TTCAACTGCGGCCTATTTGG+AGG | + | Chr2:48052809-48052828 | None:intergenic | 50.0% | |
| TGGTGCTCCGTCCTTGTCCA+TGG | + | Chr2:48052541-48052560 | None:intergenic | 60.0% | |
| ! | ATGGACAAGGACGGAGCACC+AGG | - | Chr2:48052540-48052559 | MsG0280009219.01.T01:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 48052505 | 48052948 | 48052505 | ID=MsG0280009219.01;Name=MsG0280009219.01 |
| Chr2 | mRNA | 48052505 | 48052948 | 48052505 | ID=MsG0280009219.01.T01;Parent=MsG0280009219.01;Name=MsG0280009219.01.T01;_AED=0.44;_eAED=0.44;_QI=0|0|0|1|0|0|2|0|91 |
| Chr2 | exon | 48052718 | 48052948 | 48052718 | ID=MsG0280009219.01.T01:exon:44692;Parent=MsG0280009219.01.T01 |
| Chr2 | exon | 48052505 | 48052549 | 48052505 | ID=MsG0280009219.01.T01:exon:44691;Parent=MsG0280009219.01.T01 |
| Chr2 | CDS | 48052718 | 48052948 | 48052718 | ID=MsG0280009219.01.T01:cds;Parent=MsG0280009219.01.T01 |
| Chr2 | CDS | 48052505 | 48052549 | 48052505 | ID=MsG0280009219.01.T01:cds;Parent=MsG0280009219.01.T01 |
| Gene Sequence |
| Protein sequence |
