AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280009523.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009523.01.T01 MTR_4g057975 72.289 166 38 2 1 164 1 160 3.09e-82 241
MsG0280009523.01.T01 MTR_6g075990 72.289 166 44 2 1 164 19 184 3.53e-80 238
MsG0280009523.01.T01 MTR_8g009330 70.909 165 46 2 1 164 24 187 1.85e-78 233
MsG0280009523.01.T01 MTR_5g031650 69.231 169 43 3 5 164 20 188 8.77e-78 231
MsG0280009523.01.T01 MTR_4g119600 70.482 166 47 2 1 164 1 166 2.04e-75 229
MsG0280009523.01.T01 MTR_8g463940 68.345 139 42 1 1 137 31 169 5.83e-62 189
MsG0280009523.01.T01 MTR_0219s0080 78.151 119 25 1 1 119 1 118 1.14e-60 184
MsG0280009523.01.T01 MTR_6g019000 76.667 120 27 1 1 119 1 120 1.46e-59 181
MsG0280009523.01.T01 MTR_0341s0040 76.667 120 27 1 1 119 1 120 2.49e-59 181
MsG0280009523.01.T01 MTR_7g060350 61.212 165 42 2 1 164 1 144 4.52e-59 182
MsG0280009523.01.T01 MTR_2g030885 75.610 123 30 0 1 123 1 123 5.30e-59 192
MsG0280009523.01.T01 MTR_3g067290 63.636 165 35 3 1 164 28 168 2.03e-58 181
MsG0280009523.01.T01 MTR_0340s0010 75.833 120 28 1 1 119 43 162 2.46e-58 180
MsG0280009523.01.T01 MTR_5g098640 77.193 114 26 0 1 114 1 114 1.27e-57 178
MsG0280009523.01.T01 MTR_3g110070 71.538 130 36 1 1 129 1 130 1.70e-57 176
MsG0280009523.01.T01 MTR_6g037570 71.631 141 38 2 1 139 16 156 6.88e-57 179
MsG0280009523.01.T01 MTR_2g026605 75.000 120 29 1 1 119 1 120 8.33e-57 174
MsG0280009523.01.T01 MTR_8g103800 57.927 164 41 2 1 164 1 136 7.84e-56 177
MsG0280009523.01.T01 MTR_6g018990 58.182 165 38 2 1 164 1 135 1.51e-55 171
MsG0280009523.01.T01 MTR_1g098990 59.394 165 36 2 1 164 1 135 5.67e-55 175
MsG0280009523.01.T01 MTR_3g434990 72.727 121 31 2 1 119 15 135 1.29e-52 164
MsG0280009523.01.T01 MTR_8g095070 72.269 119 33 0 1 119 1 119 6.21e-52 166
MsG0280009523.01.T01 MTR_8g059020 74.107 112 28 1 1 111 92 203 1.99e-50 162
MsG0280009523.01.T01 MTR_8g107490 71.569 102 28 1 64 164 44 145 5.05e-47 155
MsG0280009523.01.T01 MTR_4g051612 77.419 93 21 0 27 119 43 135 1.99e-46 150
MsG0280009523.01.T01 MTR_0116s0050 57.447 141 25 2 21 161 5 110 7.70e-45 145
MsG0280009523.01.T01 MTR_6g007833 73.196 97 26 0 1 97 1 97 8.69e-43 141
MsG0280009523.01.T01 MTR_4g051638 50.617 162 62 6 2 160 80 226 4.57e-41 138
MsG0280009523.01.T01 MTR_7g050385 55.797 138 57 4 27 162 84 219 3.65e-39 133
MsG0280009523.01.T01 MTR_3g015530 48.795 166 73 5 1 164 1 156 4.87e-38 130
MsG0280009523.01.T01 MTR_2g067720 75.000 84 20 1 37 119 32 115 9.23e-38 126
MsG0280009523.01.T01 MTR_2g105460 69.231 104 26 1 3 106 40 137 9.73e-38 136
MsG0280009523.01.T01 MTR_3g072200 50.932 161 57 7 2 160 8 148 6.25e-36 130
MsG0280009523.01.T01 MTR_1g059880 45.342 161 56 5 2 160 44 174 9.40e-36 123
MsG0280009523.01.T01 MTR_3g437000 55.462 119 49 4 27 143 11 127 3.16e-34 117
MsG0280009523.01.T01 MTR_4g049670 44.578 166 51 5 1 164 1 127 9.92e-32 110
MsG0280009523.01.T01 MTR_2g096070 71.250 80 22 1 64 142 83 162 1.48e-31 119
MsG0280009523.01.T01 MTR_3g077875 36.170 188 94 4 1 164 1 186 8.07e-31 113
MsG0280009523.01.T01 MTR_5g030790 70.886 79 23 0 1 79 31 109 1.09e-30 107
MsG0280009523.01.T01 MTR_3g087440 37.766 188 91 4 1 164 1 186 2.36e-30 110
MsG0280009523.01.T01 MTR_2g073060 57.447 94 34 2 37 124 74 167 1.17e-28 104
MsG0280009523.01.T01 MTR_3g022615 66.667 84 27 1 1 83 52 135 2.30e-28 102
MsG0280009523.01.T01 MTR_7g057800 48.000 125 56 2 1 116 1 125 5.13e-28 105
MsG0280009523.01.T01 MTR_7g057800 46.512 129 59 3 6 124 127 255 2.72e-26 100
MsG0280009523.01.T01 MTR_3g034840 34.659 176 103 4 1 164 1 176 9.59e-28 103
MsG0280009523.01.T01 MTR_0599s0020 34.659 176 103 4 1 164 1 176 9.59e-28 103
MsG0280009523.01.T01 MTR_2g451070 50.000 120 51 4 1 119 7 118 2.18e-27 99.8
MsG0280009523.01.T01 MTR_1g081740 43.750 160 58 6 2 160 7 135 6.10e-27 100
MsG0280009523.01.T01 MTR_6g090600 53.933 89 40 1 77 164 1 89 1.25e-26 97.1
MsG0280009523.01.T01 MTR_4g048360 72.308 65 18 0 1 65 1 65 8.45e-26 94.7
MsG0280009523.01.T01 MTR_6g452470 57.447 94 38 2 1 93 122 214 1.27e-25 97.8
MsG0280009523.01.T01 MTR_7g064870 36.527 167 86 5 1 164 1 150 4.41e-25 95.5
MsG0280009523.01.T01 MTR_5g079560 44.545 110 58 1 58 164 73 182 5.87e-25 96.7
MsG0280009523.01.T01 MTR_3g462670 39.759 166 54 5 1 164 1 122 6.65e-25 96.3
MsG0280009523.01.T01 MTR_5g053380 75.806 62 15 0 1 62 1 62 9.25e-25 91.7
MsG0280009523.01.T01 MTR_3g455770 68.493 73 22 1 1 72 7 79 1.29e-24 92.0
MsG0280009523.01.T01 MTR_8g038805 38.012 171 42 5 1 164 1 114 2.13e-24 92.4
MsG0280009523.01.T01 MTR_5g006100 35.882 170 95 4 1 164 1 162 2.56e-24 98.6
MsG0280009523.01.T01 MTR_1g024995 70.769 65 13 1 1 65 14 72 9.21e-24 89.7
MsG0280009523.01.T01 MTR_6g464570 42.857 112 57 3 59 164 14 124 9.59e-24 91.3
MsG0280009523.01.T01 MTR_1g081020 72.131 61 17 0 1 61 32 92 9.49e-23 87.0
MsG0280009523.01.T01 MTR_5g060540 44.776 134 44 3 37 164 15 124 1.51e-22 92.8
MsG0280009523.01.T01 MTR_7g055617 72.464 69 19 0 1 69 1 69 1.80e-22 90.9
MsG0280009523.01.T01 MTR_2g026585 40.625 160 63 6 2 160 8 136 3.30e-22 91.7
MsG0280009523.01.T01 MTR_5g046160 42.308 104 59 1 59 161 40 143 7.07e-22 89.0
MsG0280009523.01.T01 MTR_8g020433 80.000 50 10 0 115 164 68 117 7.27e-21 88.6
MsG0280009523.01.T01 MTR_5g071980 44.444 135 62 5 2 131 8 134 1.87e-20 85.5
MsG0280009523.01.T01 MTR_3g045850 38.562 153 71 5 1 135 1 148 2.02e-20 82.8
MsG0280009523.01.T01 MTR_1g072340 71.930 57 16 0 1 57 34 90 3.22e-20 80.5
MsG0280009523.01.T01 MTR_1g075420 38.843 121 59 1 15 135 10 115 5.29e-20 80.9
MsG0280009523.01.T01 MTR_6g084740 52.083 96 38 3 2 97 8 95 1.40e-19 80.5
MsG0280009523.01.T01 MTR_2g067930 37.654 162 68 5 1 160 1 131 1.93e-19 81.3
MsG0280009523.01.T01 MTR_4g057300 58.730 63 25 1 103 164 1 63 4.87e-19 82.4
MsG0280009523.01.T01 MTR_3g467720 67.742 62 14 1 1 62 7 62 2.15e-18 77.0
MsG0280009523.01.T01 MTR_6g077970 70.909 55 15 1 22 75 44 98 2.63e-18 75.5
MsG0280009523.01.T01 MTR_5g060510 44.138 145 54 5 22 164 37 156 4.78e-18 77.0
MsG0280009523.01.T01 MTR_0360s0020 64.615 65 17 1 1 65 24 82 8.85e-18 73.9
MsG0280009523.01.T01 MTR_1g075860 50.820 61 30 0 54 114 44 104 8.51e-17 72.4
MsG0280009523.01.T01 MTR_8g467980 52.174 69 32 1 65 132 76 144 1.25e-16 72.4
MsG0280009523.01.T01 MTR_5g015710 45.652 92 47 1 58 146 75 166 1.46e-16 73.9
MsG0280009523.01.T01 MTR_3g116710 38.053 113 64 3 53 160 72 183 1.59e-16 74.3
MsG0280009523.01.T01 MTR_4g099080 76.087 46 11 0 2 47 8 53 2.16e-16 69.3
MsG0280009523.01.T01 MTR_1g058140 31.361 169 57 5 1 164 22 136 2.66e-16 73.6
MsG0280009523.01.T01 MTR_8g016360 35.398 113 68 3 56 164 14 125 1.09e-15 69.7
MsG0280009523.01.T01 MTR_3g030230 55.738 61 26 1 101 160 69 129 1.59e-15 70.5
MsG0280009523.01.T01 MTR_5g070810 41.667 84 46 1 84 164 14 97 1.59e-15 68.9
MsG0280009523.01.T01 MTR_1g057380 58.065 62 26 0 1 62 17 78 2.71e-15 67.4
MsG0280009523.01.T01 MTR_3g074010 79.487 39 8 0 27 65 6 44 1.53e-14 65.5
MsG0280009523.01.T01 MTR_6g089230 44.444 72 39 1 90 160 10 81 2.81e-13 65.5
MsG0280009523.01.T01 MTR_7g065910 43.243 74 39 1 94 164 24 97 9.23e-13 65.1
MsG0280009523.01.T01 MTR_6g026910 36.735 147 40 5 2 147 8 102 3.13e-12 62.8
MsG0280009523.01.T01 MTR_6g029530 32.727 110 66 1 59 160 498 607 3.42e-12 63.9
MsG0280009523.01.T01 MTR_6g065450 70.833 48 8 1 1 48 93 134 4.42e-12 61.2
MsG0280009523.01.T01 MTR_7g010970 47.692 65 31 1 103 164 1 65 9.66e-12 61.2
MsG0280009523.01.T01 MTR_1g012760 34.579 107 66 3 59 164 35 138 1.38e-11 61.2
MsG0280009523.01.T01 MTR_1g012760 34.579 107 66 3 59 164 46 149 1.60e-11 61.2
MsG0280009523.01.T01 MTR_0156s0040 46.154 65 32 1 103 164 1 65 2.04e-11 59.7
MsG0280009523.01.T01 MTR_5g046245 81.250 32 6 0 133 164 5 36 2.26e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 39 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAAGGATGACTTGCTTAAT+TGG 0.177302 2:-53616773 MsG0280009523.01.T01:CDS
GGTACGTCGTCAAGCAAATA+AGG 0.305461 2:-53616751 MsG0280009523.01.T01:CDS
TGGAGATTGAACATTCTTAA+TGG 0.306492 2:-53616552 MsG0280009523.01.T01:CDS
ATCTTTCAATTTAACGTTGT+CGG 0.336029 2:+53616794 None:intergenic
AGCTCCATCAATGTTTGGTT+TGG 0.354567 2:+53616902 None:intergenic
TGAGAACGCGGAAGATGATC+TGG 0.359312 2:-53616985 MsG0280009523.01.T01:CDS
TGGTTAGCAAATATCTTAGT+AGG 0.370024 2:-53616587 MsG0280009523.01.T01:CDS
GCGATGGAGCCGGATTTATA+AGG 0.383153 2:-53616516 None:intergenic
ACGGAAGCTCCATCAATGTT+TGG 0.403119 2:+53616897 None:intergenic
TTTGGTTTGGCTTCAACCTT+AGG 0.420594 2:+53616915 None:intergenic
TAAGGATGACTTGCTTAATT+GGG 0.454747 2:-53616772 MsG0280009523.01.T01:CDS
AGCATGTGTCCTTATAAATC+CGG 0.470502 2:+53616507 None:intergenic
GCTTCATTATATGTTGACTT+TGG 0.486342 2:-53616864 MsG0280009523.01.T01:CDS
GAAGCCAAACCAAACATTGA+TGG 0.490639 2:-53616906 MsG0280009523.01.T01:CDS
AAACTGAAGTATGAGACAAT+TGG 0.506777 2:-53616636 MsG0280009523.01.T01:CDS
AAAATGTGGGTGCATGTTCA+TGG 0.511608 2:-53616607 MsG0280009523.01.T01:CDS
CAACGTTAAATTGAAAGATA+AGG 0.514377 2:-53616790 MsG0280009523.01.T01:CDS
TCACATACTAGTCGCACCAT+TGG 0.514670 2:+53616687 None:intergenic
CTTAGTCGTGTCAACTTCGT+TGG 0.518055 2:+53616824 None:intergenic
TGGAGATGTCAAACTGCCTA+AGG 0.538085 2:-53616931 MsG0280009523.01.T01:CDS
TCACAACCATGCGATGGAGC+CGG 0.543669 2:-53616526 MsG0280009523.01.T01:CDS
TCAACTTCGTTGGCATGAAG+TGG 0.549919 2:+53616834 None:intergenic
TTAGTAGGAAAACAAAGGAA+TGG 0.550984 2:-53616572 MsG0280009523.01.T01:CDS
TATAATAATGGTTGAGAACG+CGG 0.562306 2:-53616997 None:intergenic
ATCAAGTTTGATCAATCCAA+TGG 0.566355 2:-53616703 MsG0280009523.01.T01:CDS
ATAAATCCGGCTCCATCGCA+TGG 0.580864 2:+53616520 None:intergenic
CGTCGTCAAGCAAATAAGGC+GGG 0.585237 2:-53616747 MsG0280009523.01.T01:CDS
ACAATTGGCTCAAGAAAATG+TGG 0.596703 2:-53616621 MsG0280009523.01.T01:CDS
GAGAACGCGGAAGATGATCT+GGG 0.596845 2:-53616984 MsG0280009523.01.T01:CDS
CAACTTCGTTGGCATGAAGT+GGG 0.608031 2:+53616835 None:intergenic
TAATGAAGCTTCAACACGGA+CGG 0.609817 2:+53616878 None:intergenic
TGGAGTTCACAACCATGCGA+TGG 0.616718 2:-53616532 MsG0280009523.01.T01:CDS
TGGTGCGACTAGTATGTGAA+AGG 0.629707 2:-53616683 MsG0280009523.01.T01:CDS
CGACTAGTATGTGAAAGGAG+TGG 0.647874 2:-53616678 MsG0280009523.01.T01:CDS
CTTCGTTGGCATGAAGTGGG+AGG 0.658421 2:+53616838 None:intergenic
ATATCTTAGTAGGAAAACAA+AGG 0.667648 2:-53616577 MsG0280009523.01.T01:CDS
CATATAATGAAGCTTCAACA+CGG 0.680268 2:+53616874 None:intergenic
ACGTCGTCAAGCAAATAAGG+CGG 0.684842 2:-53616748 MsG0280009523.01.T01:CDS
CAATTGGCTCAAGAAAATGT+GGG 0.702931 2:-53616620 MsG0280009523.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CCAAATTTTGTCAAAAAATA+TGG - Chr2:53616557-53616576 MsG0280009523.01.T01:CDS 20.0%
!!! CCATATTTTTTGACAAAATT+TGG + Chr2:53616560-53616579 None:intergenic 20.0%
! ATATCTTAGTAGGAAAACAA+AGG - Chr2:53616931-53616950 MsG0280009523.01.T01:CDS 25.0%
! ATCTTTCAATTTAACGTTGT+CGG + Chr2:53616717-53616736 None:intergenic 25.0%
! CAACGTTAAATTGAAAGATA+AGG - Chr2:53616718-53616737 MsG0280009523.01.T01:CDS 25.0%
AAACTGAAGTATGAGACAAT+TGG - Chr2:53616872-53616891 MsG0280009523.01.T01:CDS 30.0%
ATCAAGTTTGATCAATCCAA+TGG - Chr2:53616805-53616824 MsG0280009523.01.T01:CDS 30.0%
CATATAATGAAGCTTCAACA+CGG + Chr2:53616637-53616656 None:intergenic 30.0%
TGGAGATTGAACATTCTTAA+TGG - Chr2:53616956-53616975 MsG0280009523.01.T01:CDS 30.0%
TGGTTAGCAAATATCTTAGT+AGG - Chr2:53616921-53616940 MsG0280009523.01.T01:CDS 30.0%
TTAGTAGGAAAACAAAGGAA+TGG - Chr2:53616936-53616955 MsG0280009523.01.T01:CDS 30.0%
!! ATAAGGATGACTTGCTTAAT+TGG - Chr2:53616735-53616754 MsG0280009523.01.T01:CDS 30.0%
!! GCTTCATTATATGTTGACTT+TGG - Chr2:53616644-53616663 MsG0280009523.01.T01:CDS 30.0%
!! TAAGGATGACTTGCTTAATT+GGG - Chr2:53616736-53616755 MsG0280009523.01.T01:CDS 30.0%
!!! GTCTCATACTTCAGTTTTTT+CGG + Chr2:53616869-53616888 None:intergenic 30.0%
! ACAATTGGCTCAAGAAAATG+TGG - Chr2:53616887-53616906 MsG0280009523.01.T01:CDS 35.0%
! CAATTGGCTCAAGAAAATGT+GGG - Chr2:53616888-53616907 MsG0280009523.01.T01:CDS 35.0%
AAAATGTGGGTGCATGTTCA+TGG - Chr2:53616901-53616920 MsG0280009523.01.T01:CDS 40.0%
AGCTCCATCAATGTTTGGTT+TGG + Chr2:53616609-53616628 None:intergenic 40.0%
GAAGCCAAACCAAACATTGA+TGG - Chr2:53616602-53616621 MsG0280009523.01.T01:CDS 40.0%
TAATGAAGCTTCAACACGGA+CGG + Chr2:53616633-53616652 None:intergenic 40.0%
!! TTTGGTTTGGCTTCAACCTT+AGG + Chr2:53616596-53616615 None:intergenic 40.0%
ACGGAAGCTCCATCAATGTT+TGG + Chr2:53616614-53616633 None:intergenic 45.0%
ACGTCGTCAAGCAAATAAGG+CGG - Chr2:53616760-53616779 MsG0280009523.01.T01:CDS 45.0%
CAACTTCGTTGGCATGAAGT+GGG + Chr2:53616676-53616695 None:intergenic 45.0%
CGACTAGTATGTGAAAGGAG+TGG - Chr2:53616830-53616849 MsG0280009523.01.T01:CDS 45.0%
CTTAGTCGTGTCAACTTCGT+TGG + Chr2:53616687-53616706 None:intergenic 45.0%
GGTACGTCGTCAAGCAAATA+AGG - Chr2:53616757-53616776 MsG0280009523.01.T01:CDS 45.0%
TCAACTTCGTTGGCATGAAG+TGG + Chr2:53616677-53616696 None:intergenic 45.0%
TCACATACTAGTCGCACCAT+TGG + Chr2:53616824-53616843 None:intergenic 45.0%
TGGAGATGTCAAACTGCCTA+AGG - Chr2:53616577-53616596 MsG0280009523.01.T01:CDS 45.0%
TGGTGCGACTAGTATGTGAA+AGG - Chr2:53616825-53616844 MsG0280009523.01.T01:CDS 45.0%
ATAAATCCGGCTCCATCGCA+TGG + Chr2:53616991-53617010 None:intergenic 50.0%
CGTCGTCAAGCAAATAAGGC+GGG - Chr2:53616761-53616780 MsG0280009523.01.T01:CDS 50.0%
GAGAACGCGGAAGATGATCT+GGG - Chr2:53616524-53616543 MsG0280009523.01.T01:CDS 50.0%
TGAGAACGCGGAAGATGATC+TGG - Chr2:53616523-53616542 MsG0280009523.01.T01:CDS 50.0%
TGGAGTTCACAACCATGCGA+TGG - Chr2:53616976-53616995 MsG0280009523.01.T01:CDS 50.0%
CTTCGTTGGCATGAAGTGGG+AGG + Chr2:53616673-53616692 None:intergenic 55.0%
TCACAACCATGCGATGGAGC+CGG - Chr2:53616982-53617001 MsG0280009523.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 53616518 53617012 53616518 ID=MsG0280009523.01;Name=MsG0280009523.01
Chr2 mRNA 53616518 53617012 53616518 ID=MsG0280009523.01.T01;Parent=MsG0280009523.01;Name=MsG0280009523.01.T01;_AED=0.67;_eAED=0.67;_QI=0|-1|0|1|-1|1|1|0|164
Chr2 exon 53616518 53617012 53616518 ID=MsG0280009523.01.T01:exon:16348;Parent=MsG0280009523.01.T01
Chr2 CDS 53616518 53617012 53616518 ID=MsG0280009523.01.T01:cds;Parent=MsG0280009523.01.T01
Gene Sequence

>MsG0280009523.01.T01

ATGGTTGAGAACGCGGAAGATGATCTGGGAGAGACAAAACCAAATTTTGTCAAAAAATATGGAGATGTCAAACTGCCTAAGGTTGAAGCCAAACCAAACATTGATGGAGCTTCCGTCCGTGTTGAAGCTTCATTATATGTTGACTTTGGTGTCCTCCCACTTCATGCCAACGAAGTTGACACGACTAAGTTTTTTTCCGACAACGTTAAATTGAAAGATAAGGATGACTTGCTTAATTGGGTACGTCGTCAAGCAAATAAGGCGGGATTTACAATTTTTATACAAAGATCAAGTTTGATCAATCCAATGGTGCGACTAGTATGTGAAAGGAGTGGAGCTCACAAAGTGCCGAAAAAACTGAAGTATGAGACAATTGGCTCAAGAAAATGTGGGTGCATGTTCATGGTTAGCAAATATCTTAGTAGGAAAACAAAGGAATGGAGATTGAACATTCTTAATGGAGTTCACAACCATGCGATGGAGCCGGATTTATAA

Protein sequence

>MsG0280009523.01.T01

MVENAEDDLGETKPNFVKKYGDVKLPKVEAKPNIDGASVRVEASLYVDFGVLPLHANEVDTTKFFSDNVKLKDKDDLLNWVRRQANKAGFTIFIQRSSLINPMVRLVCERSGAHKVPKKLKYETIGSRKCGCMFMVSKYLSRKTKEWRLNILNGVHNHAMEPDL*