AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010277.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010277.01.T01 MTR_3g030700 69.231 156 35 2 1 156 155 297 2.54e-63 199
MsG0280010277.01.T01 MTR_2g081040 86.364 154 21 0 1 154 128 281 9.08e-62 195
MsG0280010277.01.T01 MTR_3g104100 87.013 154 20 0 1 154 146 299 1.03e-61 195
MsG0280010277.01.T01 MTR_3g498695 46.622 148 77 2 9 156 259 404 1.21e-33 124
MsG0280010277.01.T01 MTR_3g498695 46.622 148 77 2 9 156 259 404 1.35e-33 124
MsG0280010277.01.T01 MTR_7g083900 45.185 135 72 1 22 154 258 392 2.52e-23 95.9
MsG0280010277.01.T01 MTR_7g083900 45.185 135 72 1 22 154 258 392 2.66e-23 95.9
MsG0280010277.01.T01 MTR_1g112680 48.837 86 42 1 27 112 296 379 9.91e-19 82.8
MsG0280010277.01.T01 MTR_5g030770 54.795 73 30 1 38 107 141 213 1.29e-15 73.2
MsG0280010277.01.T01 MTR_5g030770 54.795 73 30 1 38 107 141 213 1.32e-15 73.2
MsG0280010277.01.T01 MTR_3g111290 52.000 75 33 1 39 110 159 233 1.24e-14 70.5
MsG0280010277.01.T01 MTR_4g057270 48.485 66 34 0 41 106 140 205 5.26e-14 68.9
MsG0280010277.01.T01 MTR_2g084280 42.553 94 43 2 16 106 104 189 6.19e-14 68.6
MsG0280010277.01.T01 MTR_8g064700 45.455 66 36 0 43 108 237 302 1.56e-13 67.8
MsG0280010277.01.T01 MTR_8g024790 44.444 72 36 1 37 104 274 345 1.68e-12 64.7
MsG0280010277.01.T01 MTR_4g092700 36.458 96 52 2 16 105 121 213 6.36e-12 62.8
MsG0280010277.01.T01 MTR_8g024770 32.759 116 74 1 37 148 262 377 6.70e-12 63.2
MsG0280010277.01.T01 MTR_2g038040 45.070 71 36 1 49 116 51 121 8.47e-12 62.0
MsG0280010277.01.T01 MTR_4g091910 43.836 73 41 0 39 111 36 108 1.45e-11 60.8
MsG0280010277.01.T01 MTR_4g009110 37.179 78 49 0 38 115 162 239 1.49e-11 62.0
MsG0280010277.01.T01 MTR_8g027495 46.377 69 32 1 54 117 462 530 2.23e-11 61.6
MsG0280010277.01.T01 MTR_1g072320 44.262 61 34 0 44 104 464 524 2.62e-11 61.6
MsG0280010277.01.T01 MTR_7g117670 52.727 55 26 0 54 108 406 460 3.12e-11 61.2
MsG0280010277.01.T01 MTR_4g009540 35.897 78 50 0 38 115 140 217 3.41e-11 60.8
MsG0280010277.01.T01 MTR_4g009540 35.897 78 50 0 38 115 140 217 3.77e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010277.01.T01 AT3G26744 51.852 108 52 0 47 154 304 411 1.37e-29 113
MsG0280010277.01.T01 AT3G26744 50.909 110 54 0 47 156 304 413 1.80e-29 113
MsG0280010277.01.T01 AT3G26744 50.909 110 54 0 47 156 304 413 1.80e-29 113
MsG0280010277.01.T01 AT3G26744 50.909 110 54 0 47 156 304 413 1.80e-29 113
MsG0280010277.01.T01 AT1G12860 55.319 94 37 1 47 140 264 352 2.09e-26 103
MsG0280010277.01.T01 AT1G12860 55.319 94 37 1 47 140 264 352 2.83e-26 103
MsG0280010277.01.T01 AT2G16910 44.118 102 52 1 11 112 280 376 5.31e-17 78.2
MsG0280010277.01.T01 AT5G65640 55.405 74 31 1 34 105 160 233 6.23e-15 71.2
MsG0280010277.01.T01 AT5G65640 55.405 74 31 1 34 105 160 233 1.21e-14 70.9
MsG0280010277.01.T01 AT5G10570 54.054 74 31 1 35 105 133 206 1.70e-14 70.1
MsG0280010277.01.T01 AT2G28160 40.196 102 48 3 5 106 99 187 1.82e-14 70.1
MsG0280010277.01.T01 AT2G28160 40.196 102 48 3 5 106 99 187 1.87e-14 70.1
MsG0280010277.01.T01 AT4G29930 37.778 90 55 1 49 137 53 142 9.35e-13 64.3
MsG0280010277.01.T01 AT5G57150 49.231 65 33 0 42 106 47 111 9.82e-13 64.3
MsG0280010277.01.T01 AT5G57150 49.231 65 33 0 42 106 46 110 9.90e-13 64.3
MsG0280010277.01.T01 AT5G57150 49.231 65 33 0 42 106 47 111 1.01e-12 64.3
MsG0280010277.01.T01 AT5G57150 49.231 65 33 0 42 106 46 110 1.26e-12 64.3
MsG0280010277.01.T01 AT4G29930 45.070 71 38 1 49 118 53 123 1.46e-12 63.9
MsG0280010277.01.T01 AT4G29930 50.000 58 29 0 49 106 53 110 1.54e-12 63.2
MsG0280010277.01.T01 AT4G29930 50.000 58 29 0 49 106 53 110 1.66e-12 63.5
MsG0280010277.01.T01 AT4G29930 44.444 72 39 1 49 119 53 124 1.71e-12 63.9
MsG0280010277.01.T01 AT4G29930 44.444 72 39 1 49 119 53 124 1.71e-12 63.9
MsG0280010277.01.T01 AT5G57150 47.297 74 37 1 42 113 47 120 1.72e-12 63.9
MsG0280010277.01.T01 AT4G29930 44.444 72 39 1 49 119 53 124 2.19e-12 63.5
MsG0280010277.01.T01 AT2G46510 39.506 81 49 0 25 105 370 450 5.82e-12 63.5
MsG0280010277.01.T01 AT1G63650 49.123 57 29 0 53 109 408 464 6.61e-12 63.2
MsG0280010277.01.T01 AT1G63650 49.123 57 29 0 53 109 408 464 6.61e-12 63.2
MsG0280010277.01.T01 AT1G63650 49.123 57 29 0 53 109 408 464 6.61e-12 63.2
MsG0280010277.01.T01 AT4G00870 49.057 53 27 0 54 106 253 305 7.31e-12 63.2
MsG0280010277.01.T01 AT1G10610 45.205 73 35 1 39 106 218 290 1.32e-11 62.4
MsG0280010277.01.T01 AT1G10610 45.205 73 35 1 39 106 151 223 1.41e-11 62.0
MsG0280010277.01.T01 AT1G10610 45.205 73 35 1 39 106 248 320 1.58e-11 62.0
MsG0280010277.01.T01 AT4G09820 45.312 64 32 1 43 103 353 416 2.89e-11 61.2

Find 30 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGATACCTTGGTAATCTT+TGG 0.260602 2:+67137115 None:intergenic
GTGATAAGCATTTATTGATA+TGG 0.298630 2:+67134393 None:intergenic
CATATAAATGTTTATCTTGA+AGG 0.320139 2:+67134371 None:intergenic
TGGATAAAATTTCAATACTT+AGG 0.367751 2:-67134649 MsG0280010277.01.T01:CDS
AACTCTTCCTTCATTTGAAC+TGG 0.372973 2:+67134491 None:intergenic
AAATAAGTACCTTTGTTGAT+TGG 0.383387 2:+67134438 None:intergenic
TCAGCGATGAGACTCTTTGA+TGG 0.385744 2:+67137193 None:intergenic
GTTGGGAAGATGGGGAATCA+TGG 0.425769 2:-67137343 None:intergenic
GGATAAAATTTCAATACTTA+GGG 0.426628 2:-67134648 MsG0280010277.01.T01:CDS
TTGGGAAGATGGGGAATCAT+GGG 0.481218 2:-67137342 None:intergenic
TCATTTATGCCACCGCCTAC+AGG 0.484201 2:-67134548 MsG0280010277.01.T01:CDS
CTTCCTTCATTTGAACTGGA+AGG 0.486436 2:+67134495 None:intergenic
TTATGTCTTAACAATGTGTC+AGG 0.494453 2:-67134470 MsG0280010277.01.T01:CDS
TTCCTTCATTTGAACTGGAA+GGG 0.498449 2:+67134496 None:intergenic
TATAATGCAAATAACGATGT+TGG 0.516073 2:-67137301 MsG0280010277.01.T01:CDS
AACCCTTCCAGTTCAAATGA+AGG 0.531349 2:-67134498 MsG0280010277.01.T01:CDS
ATGTGAGTTCCTGTAGGCGG+TGG 0.568867 2:+67134539 None:intergenic
TGAAAATTGTGTTGCTGAGG+GGG 0.581516 2:-67137242 MsG0280010277.01.T01:CDS
AATGCAAATAACGATGTTGG+TGG 0.589495 2:-67137298 MsG0280010277.01.T01:CDS
ATGAAAATTGTGTTGCTGAG+GGG 0.598682 2:-67137243 MsG0280010277.01.T01:CDS
GGCGGTGGCATAAATGATGT+AGG 0.607996 2:+67134554 None:intergenic
GTCTTAATGTGAGTTCCTGT+AGG 0.616718 2:+67134533 None:intergenic
GGATGCTATTGACTACTCAA+AGG 0.617862 2:-67134627 MsG0280010277.01.T01:CDS
AACTGGAAGGGTTGACATGG+TGG 0.620781 2:+67134508 None:intergenic
TCATCGCTGAAAAGAAGCGA+AGG 0.628159 2:-67137180 MsG0280010277.01.T01:CDS
TTGAACTGGAAGGGTTGACA+TGG 0.641549 2:+67134505 None:intergenic
GATGAAAATTGTGTTGCTGA+GGG 0.642802 2:-67137244 MsG0280010277.01.T01:CDS
TGATGAAAATTGTGTTGCTG+AGG 0.646257 2:-67137245 MsG0280010277.01.T01:CDS
TGTTGTGCCAAAGATTACCA+AGG 0.666150 2:-67137122 MsG0280010277.01.T01:intron
TTAATGTGAGTTCCTGTAGG+CGG 0.724433 2:+67134536 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGCTAATTATATTTAAATTA+AGG + Chr2:67136433-67136452 None:intergenic 10.0%
!! TTATTTAGCTATTTAAATTA+AGG - Chr2:67136502-67136521 MsG0280010277.01.T01:intron 10.0%
!!! ATTTTTACTGAAATATTTAA+GGG - Chr2:67136619-67136638 MsG0280010277.01.T01:intron 10.0%
!! AAATACATAAACTTGTATTA+TGG + Chr2:67137032-67137051 None:intergenic 15.0%
!! ACTATTAATGTTAAAAATCA+GGG + Chr2:67135734-67135753 None:intergenic 15.0%
!! TTTATGTGAATGATAATTTA+TGG + Chr2:67136390-67136409 None:intergenic 15.0%
!!! AAATAAAGATTTTAGTAGTT+GGG + Chr2:67136488-67136507 None:intergenic 15.0%
!!! ACTTATTCATCTTTTAAATT+TGG - Chr2:67136713-67136732 MsG0280010277.01.T01:intron 15.0%
!!! ATTAAGGATTCAAATATTTT+TGG + Chr2:67135783-67135802 None:intergenic 15.0%
!!! CTTATTCATCTTTTAAATTT+GGG - Chr2:67136714-67136733 MsG0280010277.01.T01:intron 15.0%
!!! GATTTTTACTGAAATATTTA+AGG - Chr2:67136618-67136637 MsG0280010277.01.T01:intron 15.0%
!!! TAAATAAAGATTTTAGTAGT+TGG + Chr2:67136489-67136508 None:intergenic 15.0%
!! CACTATTAATGTTAAAAATC+AGG + Chr2:67135735-67135754 None:intergenic 20.0%
!! GGATAAAATTTCAATACTTA+GGG - Chr2:67137068-67137087 MsG0280010277.01.T01:intron 20.0%
!! TGGATAAAATTTCAATACTT+AGG - Chr2:67137067-67137086 MsG0280010277.01.T01:intron 20.0%
!! TTATGTTTGTTTAAAAGAGA+AGG + Chr2:67135951-67135970 None:intergenic 20.0%
!!! ATTTTCTCCATAAATGTTTT+AGG - Chr2:67136352-67136371 MsG0280010277.01.T01:intron 20.0%
!!! TTTTCTCCATAAATGTTTTA+GGG - Chr2:67136353-67136372 MsG0280010277.01.T01:intron 20.0%
! AAATAAGTACCTTTGTTGAT+TGG + Chr2:67137281-67137300 None:intergenic 25.0%
! AACAGTCATAACATACAATT+AGG + Chr2:67134880-67134899 None:intergenic 25.0%
! ACAGTCATAACATACAATTA+GGG + Chr2:67134879-67134898 None:intergenic 25.0%
! ACATAAACTTGTATTATGGA+GGG + Chr2:67137028-67137047 None:intergenic 25.0%
! AGAAGAATAGTAATTTGGAT+TGG + Chr2:67136096-67136115 None:intergenic 25.0%
! AGCATAGAAGAATAGTAATT+TGG + Chr2:67136101-67136120 None:intergenic 25.0%
! ATCATTCCCTAAAACATTTA+TGG + Chr2:67136362-67136381 None:intergenic 25.0%
! ATTATTGATGTCAAACCTTA+AGG - Chr2:67136060-67136079 MsG0280010277.01.T01:intron 25.0%
! ATTGTCTCATACACAAATTA+AGG + Chr2:67136875-67136894 None:intergenic 25.0%
! CATAGGCTATTAACAATATT+CGG - Chr2:67136847-67136866 MsG0280010277.01.T01:intron 25.0%
! GTCATTAAAAAACATAGAAC+TGG + Chr2:67136899-67136918 None:intergenic 25.0%
! GTGATAAGCATTTATTGATA+TGG + Chr2:67137326-67137345 None:intergenic 25.0%
! TAAGGTTTGACATCAATAAT+GGG + Chr2:67136060-67136079 None:intergenic 25.0%
! TACATAAACTTGTATTATGG+AGG + Chr2:67137029-67137048 None:intergenic 25.0%
! TATAATGCAAATAACGATGT+TGG - Chr2:67134415-67134434 MsG0280010277.01.T01:CDS 25.0%
! TATTTCATTTCTTTCACAGA+TGG - Chr2:67137047-67137066 MsG0280010277.01.T01:intron 25.0%
! TCTTAAAAACCAATCAACAA+AGG - Chr2:67137269-67137288 MsG0280010277.01.T01:CDS 25.0%
! TGTTAATGCAATAAAAAGAG+CGG + Chr2:67134668-67134687 None:intergenic 25.0%
! TTAAGGTTTGACATCAATAA+TGG + Chr2:67136061-67136080 None:intergenic 25.0%
! TTGTCTCATACACAAATTAA+GGG + Chr2:67136874-67136893 None:intergenic 25.0%
!! TCTAAAGTAAGTCTATAATC+TGG + Chr2:67136570-67136589 None:intergenic 25.0%
!! TCTTATTGTCATTCTGAAAA+AGG + Chr2:67135850-67135869 None:intergenic 25.0%
!! TTTTCTTTTTCTTCCCTTTT+CGG + Chr2:67134501-67134520 None:intergenic 25.0%
!!! CAAAGTTGTTTGATTTGATT+TGG + Chr2:67134786-67134805 None:intergenic 25.0%
!!! CAACTTTGTGATTTTAATCT+TGG - Chr2:67134798-67134817 MsG0280010277.01.T01:intron 25.0%
AAAAAAAGCAGTCATACAGA+AGG + Chr2:67136677-67136696 None:intergenic 30.0%
ACAATGGTACAAGCAAATAA+TGG - Chr2:67135458-67135477 MsG0280010277.01.T01:intron 30.0%
ACATTACAAGCAAGTATTTG+CGG - Chr2:67135190-67135209 MsG0280010277.01.T01:intron 30.0%
ACTCTAAGTATGAAGACTAT+TGG - Chr2:67136024-67136043 MsG0280010277.01.T01:intron 30.0%
AGGGTAAAGTTATCATTGTT+GGG + Chr2:67135715-67135734 None:intergenic 30.0%
ATTCTTACCCAAAACCTTAA+GGG + Chr2:67135622-67135641 None:intergenic 30.0%
CAAGCTCTTATAACATTGTT+GGG + Chr2:67135077-67135096 None:intergenic 30.0%
CTCTAAGTATGAAGACTATT+GGG - Chr2:67136025-67136044 MsG0280010277.01.T01:intron 30.0%
CTTCAATAAAAACCGTAAGT+TGG - Chr2:67135996-67136015 MsG0280010277.01.T01:intron 30.0%
TAAGCACACCAATTTCAAAT+AGG - Chr2:67135922-67135941 MsG0280010277.01.T01:intron 30.0%
TACTTCACTAAATCGAGTTA+GGG + Chr2:67136141-67136160 None:intergenic 30.0%
TGTCTCATACACAAATTAAG+GGG + Chr2:67136873-67136892 None:intergenic 30.0%
TTAGAGTAGATACCAACTTA+CGG + Chr2:67136011-67136030 None:intergenic 30.0%
TTATGTCTTAACAATGTGTC+AGG - Chr2:67137246-67137265 MsG0280010277.01.T01:CDS 30.0%
TTTAACAGCTCTTACTACAT+AGG - Chr2:67136830-67136849 MsG0280010277.01.T01:intron 30.0%
! AGAATAGTAATTTGGATTGG+TGG + Chr2:67136093-67136112 None:intergenic 30.0%
! TATGATACCTTGGTAATCTT+TGG + Chr2:67134604-67134623 None:intergenic 30.0%
!! GAAGGTATCCTATTTGAAAT+TGG + Chr2:67135933-67135952 None:intergenic 30.0%
!!! AATTGATGGACTCAATTTTG+AGG - Chr2:67135515-67135534 MsG0280010277.01.T01:intron 30.0%
!!! ATTGATGGACTCAATTTTGA+GGG - Chr2:67135516-67135535 MsG0280010277.01.T01:intron 30.0%
!!! GTATGACTGCTTTTTTTATC+TGG - Chr2:67136680-67136699 MsG0280010277.01.T01:intron 30.0%
!!! TCAAGTGACATTTTTTCCAA+TGG - Chr2:67136642-67136661 MsG0280010277.01.T01:intron 30.0%
AACTCTTCCTTCATTTGAAC+TGG + Chr2:67137228-67137247 None:intergenic 35.0%
AATGCAAATAACGATGTTGG+TGG - Chr2:67134418-67134437 MsG0280010277.01.T01:CDS 35.0%
ACTCGATTTAGTGAAGTACA+AGG - Chr2:67136143-67136162 MsG0280010277.01.T01:intron 35.0%
ACTTCACTAAATCGAGTTAG+GGG + Chr2:67136140-67136159 None:intergenic 35.0%
AGCTATACACATAGATTGAC+AGG - Chr2:67136449-67136468 MsG0280010277.01.T01:intron 35.0%
AGTTATATGTCGGAGAAAAG+TGG - Chr2:67135560-67135579 MsG0280010277.01.T01:intron 35.0%
ATGAAAGACGCTCATGATTA+AGG + Chr2:67135799-67135818 None:intergenic 35.0%
ATTATGACTCTCTGTTGTTG+TGG + Chr2:67136182-67136201 None:intergenic 35.0%
CAAACGAAGTTATGATACCT+TGG + Chr2:67134614-67134633 None:intergenic 35.0%
CAGAAGGAATCAATATCCAT+TGG + Chr2:67136661-67136680 None:intergenic 35.0%
CAGGGTAAAGTTATCATTGT+TGG + Chr2:67135716-67135735 None:intergenic 35.0%
CATACAATTAGGGATGTTTG+TGG + Chr2:67134869-67134888 None:intergenic 35.0%
CATTCTTACCCAAAACCTTA+AGG + Chr2:67135623-67135642 None:intergenic 35.0%
CCAACAATGTTATAAGAGCT+TGG - Chr2:67135075-67135094 MsG0280010277.01.T01:intron 35.0%
CCAAGCTCTTATAACATTGT+TGG + Chr2:67135078-67135097 None:intergenic 35.0%
GCTATACACATAGATTGACA+GGG - Chr2:67136450-67136469 MsG0280010277.01.T01:intron 35.0%
GTACTTCACTAAATCGAGTT+AGG + Chr2:67136142-67136161 None:intergenic 35.0%
GTGAGTTATATGTCTCACAT+CGG - Chr2:67134910-67134929 MsG0280010277.01.T01:intron 35.0%
GTTTGTGGTATGATTTAGCT+CGG + Chr2:67134854-67134873 None:intergenic 35.0%
TGCAAGTGTGAGTTATATGT+CGG - Chr2:67134903-67134922 MsG0280010277.01.T01:intron 35.0%
TTATCATTGTTGGGTCATGA+GGG + Chr2:67135706-67135725 None:intergenic 35.0%
TTCCTTCATTTGAACTGGAA+GGG + Chr2:67137223-67137242 None:intergenic 35.0%
! AACTCAACTTGTTTGAGCTA+AGG + Chr2:67136999-67137018 None:intergenic 35.0%
! ACTCAACTTGTTTGAGCTAA+GGG + Chr2:67136998-67137017 None:intergenic 35.0%
! ATGAAAATTGTGTTGCTGAG+GGG - Chr2:67134473-67134492 MsG0280010277.01.T01:CDS 35.0%
! GATGAAAATTGTGTTGCTGA+GGG - Chr2:67134472-67134491 MsG0280010277.01.T01:CDS 35.0%
! TGATGAAAATTGTGTTGCTG+AGG - Chr2:67134471-67134490 MsG0280010277.01.T01:CDS 35.0%
!! ATAAGAGCTTGGTTCTAAGA+AGG - Chr2:67135086-67135105 MsG0280010277.01.T01:intron 35.0%
!! TAAGAGCTTGGTTCTAAGAA+GGG - Chr2:67135087-67135106 MsG0280010277.01.T01:intron 35.0%
!!! TAAGGTTTTGGGTAAGAATG+TGG - Chr2:67135623-67135642 MsG0280010277.01.T01:intron 35.0%
!!! TTGATGGACTCAATTTTGAG+GGG - Chr2:67135517-67135536 MsG0280010277.01.T01:intron 35.0%
AACCCTTCCAGTTCAAATGA+AGG - Chr2:67137218-67137237 MsG0280010277.01.T01:CDS 40.0%
AATGAAAGAGTTTCCGCTTG+AGG - Chr2:67135268-67135287 MsG0280010277.01.T01:intron 40.0%
ACACATATAGTGAGAGAGAG+AGG + Chr2:67135016-67135035 None:intergenic 40.0%
ACCCAAAACCTTAAGGCAAT+GGG + Chr2:67134983-67135002 None:intergenic 40.0%
ACCCAAAACCTTAAGGGAAT+GGG + Chr2:67135616-67135635 None:intergenic 40.0%
ATAACATTGTTGGGTCACGA+GGG + Chr2:67135068-67135087 None:intergenic 40.0%
ATGAAAGAGTTTCCGCTTGA+GGG - Chr2:67135269-67135288 MsG0280010277.01.T01:intron 40.0%
CACATCGGATAGAAAAGTTG+AGG - Chr2:67134925-67134944 MsG0280010277.01.T01:intron 40.0%
CACTCTTACCCAAAACCTTA+AGG + Chr2:67134990-67135009 None:intergenic 40.0%
CATTGTTGCTCTCTTCACAA+AGG + Chr2:67135226-67135245 None:intergenic 40.0%
CTCATAAACCCATTGCCTTA+AGG - Chr2:67134972-67134991 MsG0280010277.01.T01:intron 40.0%
CTTCCTTCATTTGAACTGGA+AGG + Chr2:67137224-67137243 None:intergenic 40.0%
CTTTGTGAAGAGAGCAACAA+TGG - Chr2:67135224-67135243 MsG0280010277.01.T01:intron 40.0%
GAAAATGTGTAAGAGGTTGC+GGG + Chr2:67136976-67136995 None:intergenic 40.0%
GCTAAGGGAAAATGTGTAAG+AGG + Chr2:67136983-67137002 None:intergenic 40.0%
GGAAAATGTGTAAGAGGTTG+CGG + Chr2:67136977-67136996 None:intergenic 40.0%
GGGAATCATGGGAAAAATGA+GGG - Chr2:67134385-67134404 MsG0280010277.01.T01:CDS 40.0%
GTCTTAATGTGAGTTCCTGT+AGG + Chr2:67137186-67137205 None:intergenic 40.0%
GTTATCATTGTTGGGTCATG+AGG + Chr2:67135707-67135726 None:intergenic 40.0%
TACCCAAAACCTTAAGGCAA+TGG + Chr2:67134984-67135003 None:intergenic 40.0%
TACCCAAAACCTTAAGGGAA+TGG + Chr2:67135617-67135636 None:intergenic 40.0%
TATAACATTGTTGGGTCACG+AGG + Chr2:67135069-67135088 None:intergenic 40.0%
TATATGTCGGAGAAAAGTGG+AGG - Chr2:67135563-67135582 MsG0280010277.01.T01:intron 40.0%
TCGGTACAAGTAAGTCATTG+CGG - Chr2:67135355-67135374 MsG0280010277.01.T01:intron 40.0%
TGTTGTGCCAAAGATTACCA+AGG - Chr2:67134594-67134613 MsG0280010277.01.T01:CDS 40.0%
TTAATGTGAGTTCCTGTAGG+CGG + Chr2:67137183-67137202 None:intergenic 40.0%
! AACCCATTCCCTTAAGGTTT+TGG - Chr2:67135611-67135630 MsG0280010277.01.T01:intron 40.0%
! AACCCATTGCCTTAAGGTTT+TGG - Chr2:67134978-67134997 MsG0280010277.01.T01:intron 40.0%
! ACCCATTCCCTTAAGGTTTT+GGG - Chr2:67135612-67135631 MsG0280010277.01.T01:intron 40.0%
! ACCCATTGCCTTAAGGTTTT+GGG - Chr2:67134979-67134998 MsG0280010277.01.T01:intron 40.0%
! ACCTTAAGGGAATGGGTTTA+TGG + Chr2:67135609-67135628 None:intergenic 40.0%
! ATTGGTGGTCATATGCCTTA+AGG + Chr2:67136078-67136097 None:intergenic 40.0%
! CCTTAAGGGAATGGGTTTAT+GGG + Chr2:67135608-67135627 None:intergenic 40.0%
! GGATGCTATTGACTACTCAA+AGG - Chr2:67137089-67137108 MsG0280010277.01.T01:intron 40.0%
! TGAAAATTGTGTTGCTGAGG+GGG - Chr2:67134474-67134493 MsG0280010277.01.T01:CDS 40.0%
!!! TGATGGACTCAATTTTGAGG+GGG - Chr2:67135518-67135537 MsG0280010277.01.T01:intron 40.0%
AGCAAGTATTTGCGGATGAC+CGG - Chr2:67135198-67135217 MsG0280010277.01.T01:intron 45.0%
AGGGTGTGTACATACTCCTT+GGG - Chr2:67135106-67135125 MsG0280010277.01.T01:intron 45.0%
ATGAAAGAGCTTCCGCTTGA+GGG - Chr2:67135481-67135500 MsG0280010277.01.T01:intron 45.0%
CCCATAAACCCATTCCCTTA+AGG - Chr2:67135605-67135624 MsG0280010277.01.T01:intron 45.0%
GGGGAATCATGGGAAAAATG+AGG - Chr2:67134384-67134403 MsG0280010277.01.T01:CDS 45.0%
TCAAGAGGACTTTAGACCCA+AGG + Chr2:67135125-67135144 None:intergenic 45.0%
TCAGCGATGAGACTCTTTGA+TGG + Chr2:67134526-67134545 None:intergenic 45.0%
TCATCGCTGAAAAGAAGCGA+AGG - Chr2:67134536-67134555 MsG0280010277.01.T01:CDS 45.0%
TGAAAGAGTTTCCGCTTGAG+GGG - Chr2:67135270-67135289 MsG0280010277.01.T01:intron 45.0%
TGCTCTCTTCACAAAGGAAC+CGG + Chr2:67135220-67135239 None:intergenic 45.0%
TGGCGTCTCTCTTACTTATG+CGG - Chr2:67135643-67135662 MsG0280010277.01.T01:intron 45.0%
TGTGAAGAGAGCAGCAACAA+TGG - Chr2:67135442-67135461 MsG0280010277.01.T01:intron 45.0%
TTGAACTGGAAGGGTTGACA+TGG + Chr2:67137214-67137233 None:intergenic 45.0%
! AAGGGTGTGTACATACTCCT+TGG - Chr2:67135105-67135124 MsG0280010277.01.T01:intron 45.0%
! GGTCTAAAGTCCTCTTGAGA+AGG - Chr2:67135127-67135146 MsG0280010277.01.T01:intron 45.0%
!! GAAGAATTGATGGACTCACG+CGG - Chr2:67135405-67135424 MsG0280010277.01.T01:intron 45.0%
!! TGTTGCTGCTTTCTTCGCAA+CGG + Chr2:67135170-67135189 None:intergenic 45.0%
AACTGGAAGGGTTGACATGG+TGG + Chr2:67137211-67137230 None:intergenic 50.0%
AGAGGTTGCGGGTTCAAACA+AGG + Chr2:67136965-67136984 None:intergenic 50.0%
CATTGTTGGGTCATGAGGGA+AGG + Chr2:67135702-67135721 None:intergenic 50.0%
GAAAGAGTTTCCGCTTGAGG+GGG - Chr2:67135271-67135290 MsG0280010277.01.T01:intron 50.0%
GAGGTTGCGGGTTCAAACAA+GGG + Chr2:67136964-67136983 None:intergenic 50.0%
GATGAAAGAGCTTCCGCTTG+AGG - Chr2:67135480-67135499 MsG0280010277.01.T01:intron 50.0%
GGCGGTGGCATAAATGATGT+AGG + Chr2:67137165-67137184 None:intergenic 50.0%
GGGGGAGCTAGATGAATTGA+TGG - Chr2:67135501-67135520 MsG0280010277.01.T01:intron 50.0%
TCATTTATGCCACCGCCTAC+AGG - Chr2:67137168-67137187 MsG0280010277.01.T01:CDS 50.0%
TGAAAGAGCTTCCGCTTGAG+GGG - Chr2:67135482-67135501 MsG0280010277.01.T01:intron 50.0%
TGCTTTCTTCGCAACGGAAC+CGG + Chr2:67135164-67135183 None:intergenic 50.0%
!! CTCTTGAGAAGGTGTTCAGG+CGG - Chr2:67135138-67135157 MsG0280010277.01.T01:intron 50.0%
!! GAGGAATTGATGGACTCACG+CGG - Chr2:67135299-67135318 MsG0280010277.01.T01:intron 50.0%
!! GTCCTCTTGAGAAGGTGTTC+AGG - Chr2:67135135-67135154 MsG0280010277.01.T01:intron 50.0%
ATGTGAGTTCCTGTAGGCGG+TGG + Chr2:67137180-67137199 None:intergenic 55.0%
CGCCTGAACACCTTCTCAAG+AGG + Chr2:67135140-67135159 None:intergenic 55.0%
CGGAAAGAGCTTCCGCTTGA+GGG - Chr2:67135375-67135394 MsG0280010277.01.T01:intron 55.0%
CGTTGCTGCTCTCTTCGCAA+CGG + Chr2:67135335-67135354 None:intergenic 55.0%
GAAAGAGCTTCCGCTTGAGG+GGG - Chr2:67135377-67135396 MsG0280010277.01.T01:intron 55.0%
GGAAAGAGCTTCCGCTTGAG+GGG - Chr2:67135376-67135395 MsG0280010277.01.T01:intron 55.0%
TGCGAAGAGAGCAGCAACGT+CGG - Chr2:67135336-67135355 MsG0280010277.01.T01:intron 55.0%
TTGCTGAGGGGGACCGAAAA+GGG - Chr2:67134485-67134504 MsG0280010277.01.T01:CDS 55.0%
! GGGGGAGCACGAAGAATTGA+TGG - Chr2:67135395-67135414 MsG0280010277.01.T01:intron 55.0%
! GGGGGAGCATGAGGAATTGA+TGG - Chr2:67135289-67135308 MsG0280010277.01.T01:intron 55.0%
! TCATCTAGCTCCCCCTCAAG+CGG + Chr2:67135496-67135515 None:intergenic 55.0%
CGGTTGCTCCACCTTGACGT+GGG - Chr2:67135663-67135682 MsG0280010277.01.T01:intron 60.0%
GCGGAAAGAGCTTCCGCTTG+AGG - Chr2:67135374-67135393 MsG0280010277.01.T01:intron 60.0%
GTCATGAGGGAAGGAGCCAG+TGG + Chr2:67135693-67135712 None:intergenic 60.0%
GTTGCTGAGGGGGACCGAAA+AGG - Chr2:67134484-67134503 MsG0280010277.01.T01:CDS 60.0%
TCCTCATGCTCCCCCTCAAG+CGG + Chr2:67135284-67135303 None:intergenic 60.0%
TCTTCGTGCTCCCCCTCAAG+CGG + Chr2:67135390-67135409 None:intergenic 60.0%
TTGCTCCACCTTGACGTGGG+TGG - Chr2:67135666-67135685 MsG0280010277.01.T01:intron 60.0%
!! TGAGAAGGTGTTCAGGCGGC+CGG - Chr2:67135142-67135161 MsG0280010277.01.T01:intron 60.0%
CCAGTGGACCACCCACGTCA+AGG + Chr2:67135677-67135696 None:intergenic 65.0%
CCTTGACGTGGGTGGTCCAC+TGG - Chr2:67135674-67135693 MsG0280010277.01.T01:intron 65.0%
GCGGTTGCTCCACCTTGACG+TGG - Chr2:67135662-67135681 MsG0280010277.01.T01:intron 65.0%
GTGGACCACCCACGTCAAGG+TGG + Chr2:67135674-67135693 None:intergenic 65.0%
GTTGGGTCACGAGGGCAGCT+GGG + Chr2:67135060-67135079 None:intergenic 65.0%
TGTTGGGTCACGAGGGCAGC+TGG + Chr2:67135061-67135080 None:intergenic 65.0%
TTGGGTCACGAGGGCAGCTG+GGG + Chr2:67135059-67135078 None:intergenic 65.0%
! TCCGCTTGAGGGGGAGCATG+AGG - Chr2:67135280-67135299 MsG0280010277.01.T01:intron 65.0%
TGGGTCACGAGGGCAGCTGG+GGG + Chr2:67135058-67135077 None:intergenic 70.0%
GGGTCACGAGGGCAGCTGGG+GGG + Chr2:67135057-67135076 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr2 gene 67134382 67137356 67134382 ID=MsG0280010277.01;Name=MsG0280010277.01
Chr2 mRNA 67134382 67137356 67134382 ID=MsG0280010277.01.T01;Parent=MsG0280010277.01;Name=MsG0280010277.01.T01;_AED=0.32;_eAED=0.32;_QI=0|0|0|1|1|1|2|0|174
Chr2 exon 67137123 67137356 67137123 ID=MsG0280010277.01.T01:exon:14424;Parent=MsG0280010277.01.T01
Chr2 exon 67134382 67134672 67134382 ID=MsG0280010277.01.T01:exon:14423;Parent=MsG0280010277.01.T01
Chr2 CDS 67137123 67137356 67137123 ID=MsG0280010277.01.T01:cds;Parent=MsG0280010277.01.T01
Chr2 CDS 67134382 67134672 67134382 ID=MsG0280010277.01.T01:cds;Parent=MsG0280010277.01.T01
Gene Sequence

>MsG0280010277.01.T01

ATGGGGAATCATGGGAAAAATGAGGGCAATAATTATAATGCAAATAACGATGTTGGTGGTAGTAATAATAACATCATAGATAATAATGATGATGAAAATTGTGTTGCTGAGGGGGACCGAAAAGGGAAGAAAAAGAAAATGCCATCAAAGAGTCTCATCGCTGAAAAGAAGCGAAGGCAGAAACTCAGCGATAATATGCACAAGCTTCGTTCTGTTGTGCCAAAGATTACCAAGATGGATAAAATTTCAATACTTAGGGATGCTATTGACTACTCAAAGGAGTTACAAAAACAAATTAATGATCTTCAAAGTGAGATAGAGTCAAGCTCTCCTACATCATTTATGCCACCGCCTACAGGAACTCACATTAAGACATCCACCATGTCAACCCTTCCAGTTCAAATGAAGGAAGAGTTATGTCTTAACAATGTGTCAGGTCTTAAAAACCAATCAACAAAGGTACTTATTTACTTTAATAGAAAGTTCTTATTCCATATCAATAAATGCTTATCACCTTCAAGATAA
Protein sequence

>MsG0280010277.01.T01

MGNHGKNEGNNYNANNDVGGSNNNIIDNNDDENCVAEGDRKGKKKKMPSKSLIAEKKRRQKLSDNMHKLRSVVPKITKMDKISILRDAIDYSKELQKQINDLQSEIESSSPTSFMPPPTGTHIKTSTMSTLPVQMKEELCLNNVSGLKNQSTKVLIYFNRKFLFHINKCLSPSR*