AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010452.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010452.01.T04 MTR_2g084495 92.053 151 12 0 1 151 1 151 5.10e-101 290
MsG0280010452.01.T04 MTR_2g060500 71.223 139 40 0 1 139 7 145 6.90e-69 205
MsG0280010452.01.T04 MTR_2g060540 70.504 139 41 0 1 139 7 145 2.28e-68 204
MsG0280010452.01.T04 MTR_2g084525 66.187 139 47 0 1 139 1 139 3.48e-64 197
MsG0280010452.01.T04 MTR_2g084510 58.667 150 62 0 2 151 1 150 1.69e-58 182
MsG0280010452.01.T04 MTR_5g030130 47.619 147 67 3 3 149 34 170 5.20e-42 138
MsG0280010452.01.T04 MTR_5g030130 47.826 138 62 4 3 140 34 161 2.62e-40 135
MsG0280010452.01.T04 MTR_1g062160 50.000 140 60 4 2 140 4 134 6.42e-40 134
MsG0280010452.01.T04 MTR_5g007230 45.714 140 66 3 1 140 18 147 1.11e-38 130
MsG0280010452.01.T04 MTR_7g102790 47.552 143 64 5 1 141 4 137 1.52e-37 127
MsG0280010452.01.T04 MTR_4g078170 45.652 138 65 3 3 140 14 141 3.73e-37 127
MsG0280010452.01.T04 MTR_8g066820 44.928 138 66 3 3 140 32 159 1.90e-36 125
MsG0280010452.01.T04 MTR_2g083720 53.390 118 17 2 1 118 3 82 4.42e-30 105
MsG0280010452.01.T04 MTR_2g083710 79.661 59 12 0 2 60 4 62 7.77e-29 101
MsG0280010452.01.T04 MTR_2g055210 38.158 152 79 6 6 157 104 240 3.24e-25 99.0
MsG0280010452.01.T04 MTR_4g094680 38.971 136 78 4 6 141 24 154 1.10e-23 92.8
MsG0280010452.01.T04 MTR_4g116300 37.500 152 81 5 6 157 8 145 1.26e-21 87.8
MsG0280010452.01.T04 MTR_2g036170 38.971 136 66 5 6 141 39 157 4.29e-18 78.6
MsG0280010452.01.T04 MTR_8g105930 28.378 148 89 5 6 140 15 158 3.63e-12 62.4
MsG0280010452.01.T03 MTR_2g084495 89.326 178 13 1 4 181 59 230 2.59e-100 289
MsG0280010452.01.T03 MTR_2g084510 53.476 187 76 4 4 181 58 242 5.37e-57 179
MsG0280010452.01.T03 MTR_2g084525 57.219 187 69 3 4 181 59 243 9.46e-54 171
MsG0280010452.01.T03 MTR_2g060500 66.667 81 27 0 4 84 65 145 6.13e-31 110
MsG0280010452.01.T03 MTR_2g060540 65.432 81 28 0 4 84 65 145 1.80e-30 108
MsG0280010452.01.T03 MTR_2g083720 75.862 58 14 0 7 64 26 83 1.02e-24 92.0
MsG0280010452.01.T03 MTR_1g062160 34.409 186 68 6 4 180 61 201 7.06e-22 88.2
MsG0280010452.01.T03 MTR_7g102790 36.667 180 71 8 4 180 63 202 1.54e-19 82.4
MsG0280010452.01.T03 MTR_5g030130 41.905 105 48 2 4 103 88 184 3.00e-19 82.0
MsG0280010452.01.T03 MTR_5g030130 46.914 81 35 1 4 84 88 160 3.29e-18 78.2
MsG0280010452.01.T03 MTR_5g007230 45.122 82 37 1 4 85 74 147 5.02e-18 78.6
MsG0280010452.01.T03 MTR_8g066820 45.122 82 37 1 4 85 86 159 3.82e-17 76.6
MsG0280010452.01.T03 MTR_4g078170 44.048 84 39 1 2 85 66 141 1.97e-16 74.3
MsG0280010452.01.T03 MTR_4g094680 27.119 177 111 5 4 178 74 234 9.52e-12 62.0
MsG0280010452.01.T03 MTR_2g055210 29.798 198 106 8 4 180 154 339 1.03e-11 62.8
MsG0280010452.01.T02 MTR_2g084495 74.020 204 10 2 1 167 33 230 1.21e-87 256
MsG0280010452.01.T02 MTR_2g084510 45.755 212 67 6 2 167 33 242 8.66e-50 160
MsG0280010452.01.T02 MTR_2g084525 48.113 212 62 5 2 167 34 243 4.33e-44 146
MsG0280010452.01.T02 MTR_2g060540 71.250 80 23 0 2 81 40 119 3.77e-37 125
MsG0280010452.01.T02 MTR_2g060500 71.250 80 23 0 2 81 40 119 3.91e-37 125
MsG0280010452.01.T02 MTR_1g062160 38.012 171 97 2 1 166 35 201 3.00e-30 109
MsG0280010452.01.T02 MTR_5g007230 37.805 164 95 4 1 164 49 205 2.17e-26 99.8
MsG0280010452.01.T02 MTR_5g030130 35.227 176 96 5 1 166 63 230 4.64e-26 99.8
MsG0280010452.01.T02 MTR_7g102790 36.994 173 97 4 1 166 35 202 2.53e-25 97.1
MsG0280010452.01.T02 MTR_5g030130 46.465 99 45 2 1 99 63 153 9.62e-24 92.4
MsG0280010452.01.T02 MTR_2g083720 76.364 55 13 0 30 84 26 80 5.52e-23 87.4
MsG0280010452.01.T02 MTR_4g078170 32.727 165 109 2 1 164 43 206 7.44e-22 88.2
MsG0280010452.01.T02 MTR_8g066820 52.703 74 34 1 1 74 61 133 1.93e-21 87.4
MsG0280010452.01.T01 MTR_2g084495 91.102 236 15 1 1 236 1 230 2.49e-146 407
MsG0280010452.01.T01 MTR_2g084510 57.377 244 93 4 2 236 1 242 6.13e-91 268
MsG0280010452.01.T01 MTR_2g084525 60.000 245 87 3 1 236 1 243 1.19e-87 259
MsG0280010452.01.T01 MTR_2g060500 71.223 139 40 0 1 139 7 145 4.07e-68 206
MsG0280010452.01.T01 MTR_2g060540 70.504 139 41 0 1 139 7 145 1.43e-67 205
MsG0280010452.01.T01 MTR_1g062160 38.934 244 93 8 2 235 4 201 2.15e-45 150
MsG0280010452.01.T01 MTR_7g102790 39.583 240 99 10 1 235 4 202 2.76e-41 140
MsG0280010452.01.T01 MTR_5g030130 44.099 161 75 4 3 158 34 184 9.18e-41 140
MsG0280010452.01.T01 MTR_5g030130 48.148 135 60 3 5 139 36 160 1.85e-39 135
MsG0280010452.01.T01 MTR_5g007230 43.671 158 79 3 3 160 20 167 9.76e-39 134
MsG0280010452.01.T01 MTR_4g078170 34.199 231 114 6 3 233 14 206 4.59e-37 129
MsG0280010452.01.T01 MTR_8g066820 44.928 138 66 3 3 140 32 159 4.34e-36 127
MsG0280010452.01.T01 MTR_2g083720 52.500 120 19 2 1 120 3 84 3.13e-29 105
MsG0280010452.01.T01 MTR_2g083710 79.661 59 12 0 2 60 4 62 3.31e-28 102
MsG0280010452.01.T01 MTR_2g055210 30.159 252 145 11 3 235 100 339 5.22e-25 101
MsG0280010452.01.T01 MTR_4g094680 30.435 230 139 8 6 233 24 234 8.59e-25 98.6
MsG0280010452.01.T01 MTR_4g116300 38.571 140 75 5 6 145 8 136 5.19e-20 85.9
MsG0280010452.01.T01 MTR_2g036170 30.472 233 135 9 6 233 39 249 1.06e-19 85.1
MsG0280010452.01.T01 MTR_8g105930 29.139 151 84 6 6 140 15 158 1.62e-11 62.4
MsG0280010452.01.T05 MTR_2g084495 91.597 119 10 0 1 119 33 151 1.32e-76 226
MsG0280010452.01.T05 MTR_2g060500 70.755 106 31 0 2 107 40 145 6.03e-50 156
MsG0280010452.01.T05 MTR_2g060540 69.811 106 32 0 2 107 40 145 1.51e-49 155
MsG0280010452.01.T05 MTR_2g084525 65.094 106 37 0 2 107 34 139 1.47e-45 148
MsG0280010452.01.T05 MTR_2g084510 59.434 106 43 0 2 107 33 138 2.57e-40 134
MsG0280010452.01.T05 MTR_5g030130 47.863 117 52 2 1 117 63 170 5.11e-32 112
MsG0280010452.01.T05 MTR_5g030130 48.148 108 47 3 1 108 63 161 1.54e-30 109
MsG0280010452.01.T05 MTR_1g062160 50.926 108 45 2 1 108 35 134 7.24e-30 107
MsG0280010452.01.T05 MTR_5g007230 47.222 108 48 2 1 108 49 147 1.84e-29 106
MsG0280010452.01.T05 MTR_7g102790 49.550 111 46 4 1 109 35 137 7.95e-28 101
MsG0280010452.01.T05 MTR_8g066820 45.370 108 50 2 1 108 61 159 8.79e-28 102
MsG0280010452.01.T05 MTR_4g078170 45.370 108 50 2 1 108 43 141 1.87e-27 100
MsG0280010452.01.T05 MTR_2g083720 75.862 58 14 0 29 86 25 82 1.52e-24 89.7
MsG0280010452.01.T05 MTR_2g055210 37.600 125 64 5 1 125 130 240 7.67e-17 75.1
MsG0280010452.01.T05 MTR_4g116300 36.585 123 65 4 3 125 36 145 9.39e-14 65.9
MsG0280010452.01.T05 MTR_4g094680 36.735 98 59 2 12 109 60 154 1.91e-13 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010452.01.T04 AT1G43000 47.143 140 64 3 1 140 13 142 2.74e-38 130
MsG0280010452.01.T04 AT1G43000 47.143 140 64 3 1 140 6 135 3.14e-38 130
MsG0280010452.01.T04 AT4G17900 48.921 139 60 4 3 140 19 147 5.26e-38 129
MsG0280010452.01.T04 AT4G17900 48.921 139 60 4 3 140 33 161 6.91e-38 129
MsG0280010452.01.T04 AT1G32700 47.794 136 61 3 6 141 14 139 4.63e-37 127
MsG0280010452.01.T04 AT2G27930 44.737 152 75 4 6 151 6 154 9.89e-36 122
MsG0280010452.01.T04 AT2G27930 44.737 152 75 4 6 151 6 154 9.89e-36 122
MsG0280010452.01.T04 AT2G27930 46.043 139 69 3 6 141 6 141 7.86e-35 120
MsG0280010452.01.T04 AT2G27930 46.043 139 69 3 6 141 6 141 7.86e-35 120
MsG0280010452.01.T04 AT1G21000 45.255 137 65 4 5 141 14 140 1.42e-33 119
MsG0280010452.01.T04 AT1G21000 45.255 137 65 4 5 141 17 143 1.49e-33 119
MsG0280010452.01.T04 AT1G76590 43.066 137 68 4 5 141 19 145 1.52e-30 110
MsG0280010452.01.T04 AT1G32700 48.624 109 47 2 33 141 1 100 9.12e-30 107
MsG0280010452.01.T04 AT5G46710 43.796 137 66 4 6 141 30 156 2.00e-28 103
MsG0280010452.01.T04 AT5G46710 44.118 136 65 4 6 140 14 139 5.35e-28 103
MsG0280010452.01.T04 AT5G46710 44.118 136 65 4 6 140 30 155 1.06e-27 103
MsG0280010452.01.T04 AT2G12646 36.538 156 85 5 2 157 1 142 2.45e-22 89.7
MsG0280010452.01.T04 AT2G12646 36.842 152 82 5 6 157 8 145 3.54e-22 89.4
MsG0280010452.01.T04 AT2G12646 36.842 152 82 5 6 157 8 145 3.54e-22 89.4
MsG0280010452.01.T04 AT3G60670 36.129 155 85 5 3 157 6 146 6.49e-22 88.6
MsG0280010452.01.T04 AT1G31040 37.500 136 73 5 6 141 21 144 2.94e-21 86.7
MsG0280010452.01.T04 AT2G27930 45.349 86 42 2 59 141 61 144 2.47e-18 78.2
MsG0280010452.01.T04 AT2G27930 45.349 86 42 2 59 141 61 144 2.47e-18 78.2
MsG0280010452.01.T04 AT2G01818 31.655 139 88 4 6 141 13 147 7.01e-15 69.3
MsG0280010452.01.T03 AT1G43000 33.149 181 91 5 4 180 69 223 6.81e-22 89.0
MsG0280010452.01.T03 AT1G43000 33.149 181 91 5 4 180 62 216 6.92e-22 88.6
MsG0280010452.01.T03 AT1G76590 33.514 185 105 5 4 180 71 245 2.92e-21 87.8
MsG0280010452.01.T03 AT1G21000 32.086 187 108 6 4 180 66 243 1.07e-19 83.6
MsG0280010452.01.T03 AT1G21000 32.086 187 108 6 4 180 69 246 1.09e-19 83.6
MsG0280010452.01.T03 AT1G32700 48.276 87 37 1 4 90 26 104 3.54e-19 80.5
MsG0280010452.01.T03 AT1G32700 47.191 89 39 1 4 92 65 145 3.87e-19 81.3
MsG0280010452.01.T03 AT2G27930 45.349 86 42 2 4 86 58 141 6.98e-18 77.4
MsG0280010452.01.T03 AT2G27930 45.349 86 42 2 4 86 58 141 6.98e-18 77.4
MsG0280010452.01.T03 AT4G17900 47.872 94 38 3 2 94 85 168 8.46e-18 78.2
MsG0280010452.01.T03 AT2G27930 45.349 86 42 2 4 86 61 144 1.32e-17 77.0
MsG0280010452.01.T03 AT2G27930 45.349 86 42 2 4 86 61 144 1.32e-17 77.0
MsG0280010452.01.T03 AT4G17900 47.872 94 38 3 2 94 71 154 2.15e-17 76.6
MsG0280010452.01.T03 AT2G27930 45.238 84 41 2 4 84 58 139 7.21e-17 74.3
MsG0280010452.01.T03 AT2G27930 45.238 84 41 2 4 84 58 139 7.21e-17 74.3
MsG0280010452.01.T03 AT5G46710 32.418 182 85 7 2 180 63 209 4.32e-13 65.1
MsG0280010452.01.T03 AT5G46710 45.238 84 37 2 2 84 79 154 5.49e-13 65.1
MsG0280010452.01.T03 AT5G46710 45.238 84 37 2 2 84 79 154 8.31e-13 63.5
MsG0280010452.01.T02 AT1G32700 35.294 170 103 3 1 166 1 167 6.53e-26 97.4
MsG0280010452.01.T02 AT1G43000 35.294 187 93 7 1 166 37 216 6.63e-26 98.6
MsG0280010452.01.T02 AT1G43000 35.294 187 93 7 1 166 44 223 6.89e-26 99.0
MsG0280010452.01.T02 AT1G32700 35.294 170 103 3 1 166 40 206 1.76e-25 97.4
MsG0280010452.01.T02 AT4G17900 36.047 172 93 5 1 164 48 210 3.35e-25 96.7
MsG0280010452.01.T02 AT4G17900 58.108 74 30 1 1 74 62 134 5.87e-25 96.7
MsG0280010452.01.T02 AT5G46710 36.782 174 98 6 1 166 40 209 5.11e-22 88.6
MsG0280010452.01.T02 AT2G27930 38.922 167 90 5 1 166 32 187 7.28e-22 87.4
MsG0280010452.01.T02 AT2G27930 38.922 167 90 5 1 166 32 187 7.28e-22 87.4
MsG0280010452.01.T02 AT5G46710 36.782 174 98 6 1 166 56 225 9.61e-22 88.2
MsG0280010452.01.T02 AT1G21000 28.780 205 105 5 1 166 44 246 2.52e-21 87.4
MsG0280010452.01.T02 AT1G21000 28.780 205 105 5 1 166 41 243 2.62e-21 87.4
MsG0280010452.01.T02 AT5G46710 54.054 74 33 1 1 74 56 128 4.52e-20 82.4
MsG0280010452.01.T02 AT2G27930 50.000 78 37 1 1 78 32 107 2.68e-19 80.5
MsG0280010452.01.T02 AT2G27930 50.000 78 37 1 1 78 32 107 2.68e-19 80.5
MsG0280010452.01.T02 AT1G76590 28.571 203 105 5 1 166 46 245 3.08e-19 82.0
MsG0280010452.01.T01 AT1G43000 36.402 239 120 7 1 235 13 223 9.77e-44 147
MsG0280010452.01.T01 AT1G43000 36.402 239 120 7 1 235 6 216 1.06e-43 147
MsG0280010452.01.T01 AT1G21000 35.270 241 135 8 5 235 17 246 3.79e-40 138
MsG0280010452.01.T01 AT1G21000 35.270 241 135 8 5 235 14 243 4.07e-40 138
MsG0280010452.01.T01 AT4G17900 47.973 148 64 5 3 149 33 168 1.31e-38 134
MsG0280010452.01.T01 AT1G76590 35.146 239 135 7 5 235 19 245 1.95e-38 134
MsG0280010452.01.T01 AT4G17900 47.973 148 64 5 3 149 19 154 3.33e-38 132
MsG0280010452.01.T01 AT1G32700 47.183 142 65 3 6 147 14 145 8.31e-38 131
MsG0280010452.01.T01 AT2G27930 46.043 139 69 3 6 141 6 141 9.14e-35 123
MsG0280010452.01.T01 AT2G27930 46.043 139 69 3 6 141 6 141 9.14e-35 123
MsG0280010452.01.T01 AT2G27930 45.985 137 68 3 6 139 6 139 8.94e-34 120
MsG0280010452.01.T01 AT2G27930 45.985 137 68 3 6 139 6 139 8.94e-34 120
MsG0280010452.01.T01 AT1G32700 47.826 115 51 2 33 147 1 106 2.72e-30 111
MsG0280010452.01.T01 AT5G46710 34.052 232 115 8 6 235 14 209 6.20e-28 105
MsG0280010452.01.T01 AT5G46710 43.704 135 65 4 6 139 30 154 9.44e-28 105
MsG0280010452.01.T01 AT5G46710 43.704 135 65 4 6 139 30 154 1.40e-27 103
MsG0280010452.01.T01 AT3G60670 33.511 188 107 7 3 183 6 182 2.67e-22 92.0
MsG0280010452.01.T01 AT1G31040 29.167 240 142 9 6 235 21 242 1.43e-21 90.1
MsG0280010452.01.T01 AT2G12646 38.571 140 75 5 6 145 8 136 2.74e-21 89.7
MsG0280010452.01.T01 AT2G12646 38.571 140 75 5 6 145 8 136 2.74e-21 89.7
MsG0280010452.01.T01 AT2G12646 38.571 140 75 5 6 145 5 133 2.76e-21 89.7
MsG0280010452.01.T01 AT2G27930 45.349 86 42 2 59 141 61 144 5.75e-17 76.6
MsG0280010452.01.T01 AT2G27930 45.349 86 42 2 59 141 61 144 5.75e-17 76.6
MsG0280010452.01.T01 AT2G01818 28.632 234 140 8 6 236 13 222 5.97e-16 74.7
MsG0280010452.01.T05 AT1G43000 49.541 109 46 2 1 109 37 136 5.62e-30 107
MsG0280010452.01.T05 AT1G43000 49.541 109 46 2 1 109 44 143 5.71e-30 107
MsG0280010452.01.T05 AT1G32700 48.624 109 47 2 1 109 1 100 8.06e-30 105
MsG0280010452.01.T05 AT4G17900 50.459 109 44 3 1 108 48 147 8.41e-30 107
MsG0280010452.01.T05 AT4G17900 50.459 109 44 3 1 108 62 161 8.66e-30 107
MsG0280010452.01.T05 AT1G32700 48.624 109 47 2 1 109 40 139 1.89e-29 106
MsG0280010452.01.T05 AT2G27930 44.800 125 61 3 1 119 32 154 8.19e-29 103
MsG0280010452.01.T05 AT2G27930 44.800 125 61 3 1 119 32 154 8.19e-29 103
MsG0280010452.01.T05 AT2G27930 46.429 112 55 2 1 109 32 141 9.36e-28 101
MsG0280010452.01.T05 AT2G27930 46.429 112 55 2 1 109 32 141 9.36e-28 101
MsG0280010452.01.T05 AT1G21000 46.789 109 49 3 1 109 41 140 4.22e-25 95.5
MsG0280010452.01.T05 AT1G21000 46.789 109 49 3 1 109 44 143 4.57e-25 95.5
MsG0280010452.01.T05 AT1G76590 45.370 108 50 3 1 108 46 144 2.82e-24 93.6
MsG0280010452.01.T05 AT5G46710 45.455 110 50 3 1 109 56 156 1.06e-23 90.1
MsG0280010452.01.T05 AT5G46710 45.872 109 49 3 1 108 40 139 2.20e-23 90.5
MsG0280010452.01.T05 AT5G46710 45.872 109 49 3 1 108 56 155 3.91e-23 90.1
MsG0280010452.01.T05 AT2G27930 45.349 86 42 2 27 109 61 144 1.42e-18 77.4
MsG0280010452.01.T05 AT2G27930 45.349 86 42 2 27 109 61 144 1.42e-18 77.4
MsG0280010452.01.T05 AT2G12646 34.959 123 67 4 3 125 36 145 3.28e-14 67.0
MsG0280010452.01.T05 AT2G12646 34.959 123 67 4 3 125 36 145 3.28e-14 67.0
MsG0280010452.01.T05 AT2G12646 34.959 123 67 4 3 125 33 142 3.56e-14 67.0
MsG0280010452.01.T05 AT3G60670 35.965 114 61 3 12 125 45 146 1.04e-13 65.5
MsG0280010452.01.T05 AT1G31040 34.694 98 54 3 12 109 57 144 1.58e-11 59.7

Find 28 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCCCTTTCTTCTTGAATT+TGG 0.126179 2:+69839786 None:intergenic
AATCGAAATTTACAAACATT+TGG 0.317490 2:-69840530 MsG0280010452.01.T01:CDS
TACAAACATTTGGATATTCT+AGG 0.325649 2:-69840520 MsG0280010452.01.T01:CDS
TTCTTCGTAGGTTGGGAATA+AGG 0.331085 2:+69840445 None:intergenic
TATACTTGCAATTACAAATC+AGG 0.360275 2:-69840587 MsG0280010452.01.T01:intron
AAGAAGACGATAGGTATAAT+TGG 0.364201 2:-69840427 MsG0280010452.01.T01:CDS
AGTTGCTAATCACATAGGTT+TGG 0.394909 2:+69840494 None:intergenic
TGCAAAGGAGACAGAAGAAA+TGG 0.410636 2:-69839994 MsG0280010452.01.T01:CDS
AGAAGAAATGGAAAGATTGT+TGG 0.417250 2:-69839982 MsG0280010452.01.T01:CDS
TATCGTCTTCTTCGTAGGTT+GGG 0.419261 2:+69840438 None:intergenic
GCCAGAGAGCAAAAGTAATA+AGG 0.422528 2:+69840355 None:intergenic
AAAGCCAAATTCAAGAAGAA+AGG 0.446839 2:-69839790 MsG0280010452.01.T01:CDS
AAGGTCCTACAAGATTCCTT+TGG 0.477640 2:+69840374 None:intergenic
CTATCGTCTTCTTCGTAGGT+TGG 0.485528 2:+69840437 None:intergenic
ATATGCCAAAGGAATCTTGT+AGG 0.487935 2:-69840379 MsG0280010452.01.T01:CDS
AAGCCAAATTCAAGAAGAAA+GGG 0.497702 2:-69839789 MsG0280010452.01.T01:CDS
TGTAAAGTTGCTAATCACAT+AGG 0.533475 2:+69840489 None:intergenic
AGAAATGGAAAGATTGTTGG+AGG 0.534953 2:-69839979 MsG0280010452.01.T01:CDS
AAGAAAGGGAATACCTCGTA+GGG 0.545800 2:-69839775 MsG0280010452.01.T01:CDS
ATTGTTGGAGGAATCAATCA+AGG 0.555066 2:-69839967 MsG0280010452.01.T01:CDS
ATACCTATCGTCTTCTTCGT+AGG 0.556131 2:+69840433 None:intergenic
AATCGAAAAGTAAATGAAGC+GGG 0.558672 2:-69839903 MsG0280010452.01.T01:CDS
CAGTTGTATTCATAAACAAG+AGG 0.585618 2:-69840468 MsG0280010452.01.T01:CDS
GAAGAAAGGGAATACCTCGT+AGG 0.613671 2:-69839776 MsG0280010452.01.T01:CDS
AAATCGAAAAGTAAATGAAG+CGG 0.626418 2:-69839904 MsG0280010452.01.T01:CDS
CAACCTACGAAGAAGACGAT+AGG 0.637898 2:-69840436 MsG0280010452.01.T01:CDS
ATCTTTGTAAGATATGCCAA+AGG 0.674093 2:-69840390 MsG0280010452.01.T01:CDS
TTGCAATTACAAATCAGGTG+AGG 0.697297 2:-69840582 MsG0280010452.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTAATTAAAGAAAAG+TGG + Chr2:69840011-69840030 None:intergenic 10.0%
!! ACAAAATAAATTAAAAAAAG+AGG + Chr2:69840413-69840432 None:intergenic 10.0%
!!! TGTATAATTTTTTTGACTTT+GGG - Chr2:69840049-69840068 MsG0280010452.01.T01:intron 15.0%
!!! TTGTATAATTTTTTTGACTT+TGG - Chr2:69840048-69840067 MsG0280010452.01.T01:intron 15.0%
!! AAAAAGAGGATCTATAAAAA+AGG + Chr2:69840399-69840418 None:intergenic 20.0%
!! AATCGAAATTTACAAACATT+TGG - Chr2:69840146-69840165 MsG0280010452.01.T01:intron 20.0%
!!! CAATCGCTTAATTTTTTTAT+TGG - Chr2:69840508-69840527 MsG0280010452.01.T01:CDS 20.0%
!!! TTTTTATTGGTGTAAAGAAT+GGG - Chr2:69840521-69840540 MsG0280010452.01.T01:CDS 20.0%
!!! TTTTTTATTGGTGTAAAGAA+TGG - Chr2:69840520-69840539 MsG0280010452.01.T01:CDS 20.0%
! AAATCGAAAAGTAAATGAAG+CGG - Chr2:69840772-69840791 MsG0280010452.01.T01:CDS 25.0%
! ATAAAAAAGGAGACATGATA+AGG + Chr2:69840386-69840405 None:intergenic 25.0%
! TACAAACATTTGGATATTCT+AGG - Chr2:69840156-69840175 MsG0280010452.01.T01:intron 25.0%
! TATACTTGCAATTACAAATC+AGG - Chr2:69840089-69840108 MsG0280010452.01.T01:intron 25.0%
!!! GATTTTTTGGCTTTTTGTTT+TGG + Chr2:69840737-69840756 None:intergenic 25.0%
AAAGCCAAATTCAAGAAGAA+AGG - Chr2:69840886-69840905 MsG0280010452.01.T01:CDS 30.0%
AAGAAGACGATAGGTATAAT+TGG - Chr2:69840249-69840268 MsG0280010452.01.T01:intron 30.0%
AAGCCAAATTCAAGAAGAAA+GGG - Chr2:69840887-69840906 MsG0280010452.01.T01:CDS 30.0%
AATCGAAAAGTAAATGAAGC+GGG - Chr2:69840773-69840792 MsG0280010452.01.T01:CDS 30.0%
ACGAAGAGAATGAAGAAAAA+AGG - Chr2:69840813-69840832 MsG0280010452.01.T01:CDS 30.0%
AGAAGAAATGGAAAGATTGT+TGG - Chr2:69840694-69840713 MsG0280010452.01.T01:intron 30.0%
ATCTTTGTAAGATATGCCAA+AGG - Chr2:69840286-69840305 MsG0280010452.01.T01:intron 30.0%
ATTCCCTTTCTTCTTGAATT+TGG + Chr2:69840893-69840912 None:intergenic 30.0%
CAAGTTTGAATGCATAAAGA+AGG - Chr2:69840640-69840659 MsG0280010452.01.T01:intron 30.0%
CAGTTGTATTCATAAACAAG+AGG - Chr2:69840208-69840227 MsG0280010452.01.T01:intron 30.0%
CGAAGAGAATGAAGAAAAAA+GGG - Chr2:69840814-69840833 MsG0280010452.01.T01:CDS 30.0%
TGAATGCATAAAGAAGGATA+GGG - Chr2:69840646-69840665 MsG0280010452.01.T01:intron 30.0%
TGTAAAGTTGCTAATCACAT+AGG + Chr2:69840190-69840209 None:intergenic 30.0%
TTGAATGCATAAAGAAGGAT+AGG - Chr2:69840645-69840664 MsG0280010452.01.T01:intron 30.0%
TTTATGCAAGATCCACAAAA+TGG + Chr2:69839887-69839906 None:intergenic 30.0%
! ATTTTGTTCACGCGATTTTT+TGG + Chr2:69840750-69840769 None:intergenic 30.0%
! CAAAAATTTGCTTGCAATCA+CGG - Chr2:69839781-69839800 MsG0280010452.01.T01:CDS 30.0%
!!! CTTGAATTTGGCTTTTTGAT+AGG + Chr2:69840881-69840900 None:intergenic 30.0%
AACTTCACATGATGTGAAGA+AGG + Chr2:69839807-69839826 None:intergenic 35.0%
AGAAATGGAAAGATTGTTGG+AGG - Chr2:69840697-69840716 MsG0280010452.01.T01:intron 35.0%
AGAGAATGAAGAAAAAAGGG+AGG - Chr2:69840817-69840836 MsG0280010452.01.T01:CDS 35.0%
AGTTGCTAATCACATAGGTT+TGG + Chr2:69840185-69840204 None:intergenic 35.0%
ATATGCCAAAGGAATCTTGT+AGG - Chr2:69840297-69840316 MsG0280010452.01.T01:intron 35.0%
ATTGTTGGAGGAATCAATCA+AGG - Chr2:69840709-69840728 MsG0280010452.01.T01:intron 35.0%
CCAAGTGAGTGTTCTTTAAA+GGG - Chr2:69839934-69839953 MsG0280010452.01.T01:CDS 35.0%
GAATGCATAAAGAAGGATAG+GGG - Chr2:69840647-69840666 MsG0280010452.01.T01:intron 35.0%
TCCAAGTGAGTGTTCTTTAA+AGG - Chr2:69839933-69839952 MsG0280010452.01.T01:CDS 35.0%
TTGCAATTACAAATCAGGTG+AGG - Chr2:69840094-69840113 MsG0280010452.01.T01:intron 35.0%
! TGCAATGACAATCCATTTTG+TGG - Chr2:69839872-69839891 MsG0280010452.01.T01:CDS 35.0%
!! ACCTTATTACTTTTGCTCTC+TGG - Chr2:69840320-69840339 MsG0280010452.01.T01:intron 35.0%
AAGAAAGGGAATACCTCGTA+GGG - Chr2:69840901-69840920 MsG0280010452.01.T01:CDS 40.0%
AAGGTCCTACAAGATTCCTT+TGG + Chr2:69840305-69840324 None:intergenic 40.0%
ATACCTATCGTCTTCTTCGT+AGG + Chr2:69840246-69840265 None:intergenic 40.0%
GCCAGAGAGCAAAAGTAATA+AGG + Chr2:69840324-69840343 None:intergenic 40.0%
TAAAAAAAGGTTGCCCTACG+AGG + Chr2:69840917-69840936 None:intergenic 40.0%
TATCGTCTTCTTCGTAGGTT+GGG + Chr2:69840241-69840260 None:intergenic 40.0%
TGCAAAGGAGACAGAAGAAA+TGG - Chr2:69840682-69840701 MsG0280010452.01.T01:intron 40.0%
! CCCTTTAAAGAACACTCACT+TGG + Chr2:69839937-69839956 None:intergenic 40.0%
! TTCTTCGTAGGTTGGGAATA+AGG + Chr2:69840234-69840253 None:intergenic 40.0%
!! GCAAGCAAATTTTTGAGCCA+GGG + Chr2:69839776-69839795 None:intergenic 40.0%
!! TGCAAGCAAATTTTTGAGCC+AGG + Chr2:69839777-69839796 None:intergenic 40.0%
CAACCTACGAAGAAGACGAT+AGG - Chr2:69840240-69840259 MsG0280010452.01.T01:intron 45.0%
CTATCGTCTTCTTCGTAGGT+TGG + Chr2:69840242-69840261 None:intergenic 45.0%
CTTCTGTCTCCTTTGCACTT+AGG + Chr2:69840679-69840698 None:intergenic 45.0%
GAAGAAAGGGAATACCTCGT+AGG - Chr2:69840900-69840919 MsG0280010452.01.T01:CDS 45.0%
!! GGGCTTTTTCCTAAGTGCAA+AGG - Chr2:69840667-69840686 MsG0280010452.01.T01:intron 45.0%
!!! TTATGTTTATTAATTTTTAT+TGG - Chr2:69840480-69840499 MsG0280010452.01.T01:CDS 5.0%
! CTTTTGCTCTCTGGCATGCA+AGG - Chr2:69840329-69840348 MsG0280010452.01.T01:intron 50.0%
Chromosome Type Strat End Strand Name
Chr2 gene 69839761 69840937 69839761 ID=MsG0280010452.01;Name=MsG0280010452.01
Chr2 mRNA 69839761 69840937 69839761 ID=MsG0280010452.01.T01;Parent=MsG0280010452.01;Name=MsG0280010452.01.T01;_AED=0.04;_eAED=0.04;_QI=0|0|0|1|0|0|3|0|236
Chr2 exon 69840348 69840587 69840348 ID=MsG0280010452.01.T01:exon:9901;Parent=MsG0280010452.01.T01
Chr2 CDS 69840761 69840937 69840761 ID=MsG0280010452.01.T01:cds;Parent=MsG0280010452.01.T01
Chr2 CDS 69840348 69840587 69840348 ID=MsG0280010452.01.T01:cds;Parent=MsG0280010452.01.T01
Chr2 CDS 69839761 69840054 69839761 ID=MsG0280010452.01.T01:cds;Parent=MsG0280010452.01.T01
Chr2 mRNA 69839761 69840841 69839761 ID=MsG0280010452.01.T02;Parent=MsG0280010452.01;Name=MsG0280010452.01.T02;_AED=0.18;_eAED=0.18;_QI=0|0|0|1|0|0|3|0|167
Chr2 exon 69839761 69840017 69839761 ID=MsG0280010452.01.T02:exon:9905;Parent=MsG0280010452.01.T02
Chr2 exon 69840422 69840587 69840422 ID=MsG0280010452.01.T02:exon:9904;Parent=MsG0280010452.01.T02
Chr2 CDS 69840761 69840841 69840761 ID=MsG0280010452.01.T02:cds;Parent=MsG0280010452.01.T02
Chr2 CDS 69840422 69840587 69840422 ID=MsG0280010452.01.T02:cds;Parent=MsG0280010452.01.T02
Chr2 CDS 69839761 69840017 69839761 ID=MsG0280010452.01.T02:cds;Parent=MsG0280010452.01.T02
Chr2 mRNA 69839761 69840599 69839761 ID=MsG0280010452.01.T03;Parent=MsG0280010452.01;Name=MsG0280010452.01.T03;_AED=0.15;_eAED=0.15;_QI=0|0|0|1|0|0|2|0|181
Chr2 exon 69840348 69840599 69840348 ID=MsG0280010452.01.T03:exon:9906;Parent=MsG0280010452.01.T03
Chr2 CDS 69840348 69840599 69840348 ID=MsG0280010452.01.T03:cds;Parent=MsG0280010452.01.T03
Chr2 CDS 69839761 69840054 69839761 ID=MsG0280010452.01.T03:cds;Parent=MsG0280010452.01.T03
Chr2 mRNA 69840291 69840937 69840291 ID=MsG0280010452.01.T04;Parent=MsG0280010452.01;Name=MsG0280010452.01.T04;_AED=0.23;_eAED=0.23;_QI=0|0|0|1|0|0|2|0|157
Chr2 CDS 69840761 69840937 69840761 ID=MsG0280010452.01.T04:cds;Parent=MsG0280010452.01.T04
Chr2 CDS 69840291 69840587 69840291 ID=MsG0280010452.01.T04:cds;Parent=MsG0280010452.01.T04
Chr2 mRNA 69840291 69840841 69840291 ID=MsG0280010452.01.T05;Parent=MsG0280010452.01;Name=MsG0280010452.01.T05;_AED=0.29;_eAED=0.29;_QI=0|0|0|1|0|0|2|0|125
Chr2 CDS 69840761 69840841 69840761 ID=MsG0280010452.01.T05:cds;Parent=MsG0280010452.01.T05
Chr2 CDS 69840291 69840587 69840291 ID=MsG0280010452.01.T05:cds;Parent=MsG0280010452.01.T05
Gene Sequence

>MsG0280010452.01.T04

ATGATGTTCGTGCCCTGGCTCAAAAATTTGCTTGCAATCACGGCCTTCTTCACATCATGTGAAGTTCATCTGAATGAGTCAAAAAATGAACGCAACATGTTCTGTCTCGAATGCAATGACAATCCATTTTGTGGATCTTGCATAAAATCTCATCACAAAGATCATCGAGTAATCCAAGTGAGGAGATCATCTTATACAAACGTCATTAAGACAATCGAAATTTACAAACATTTGGATATTCTAGGAATCCAAACCTATGTGATTAGCAACTTTACAGTTGTATTCATAAACAAGAGGCCTTATTCCCAACCTACGAAGAAGACGATAGGTATAATTGGTTGCAGTAGCGACTATCTTTGTAAGATATGCCAAAGGAATCTTGTAGGACCTTATTACTTTTGCTCTCTGGCATGCAAGGTAAATTGTTTTCTCGATCTCATTTTCTTTTTCCTTATCATGTCTCCTTTTTTATAG

>MsG0280010452.01.T03

ATTACAAATCAGGTGAGGAGATCATCTTATACAAACGTCATTAAGACAATCGAAATTTACAAACATTTGGATATTCTAGGAATCCAAACCTATGTGATTAGCAACTTTACAGTTGTATTCATAAACAAGAGGCCTTATTCCCAACCTACGAAGAAGACGATAGGTATAATTGGTTGCAGTAGCGACTATCTTTGTAAGATATGCCAAAGGAATCTTGTAGGACCTTATTACTTTTGCTCTCTGGCATGCAAGTTTGAATGCATAAAGAAGGATAGGGGCTTTTTCCTAAGTGCAAAGGAGACAGAAGAAATGGAAAGATTGTTGGAGGAATCAATCAAGGCACCAAAACAAAAAGCCAAAAAATCGCGTGAACAAAATCTAAATCGAAAAGTAAATGAAGCGGGAATCAACAATGAAGAAAACGAAGAGAATGAAGAAAAAAGGGAGGAAGAAGAAAATGAACAAGAAAAAAGAGAAAACATGCTTCCTATCAAAAAGCCAAATTCAAGAAGAAAGGGAATACCTCGTAGGGCAACCTTTTTTTAA

>MsG0280010452.01.T02

ATGTTCTGTCTCGAATGCAATGACAATCCATTTTGTGGATCTTGCATAAAATCTCATCACAAAGATCATCGAGTAATCCAAGTGAGGAGATCATCTTATACAAACGTCATTAAGACAATCGAAATTTACAAACATTTGGATATTCTAGGAATCCAAACCTATGTGATTAGCAACTTTACAGTTGTATTCATAAACAAGAGGCCTTATTCCCAACCTACGAAGAAGACGATAGGTATAATTGGTTGCAGTGCAAAGGAGACAGAAGAAATGGAAAGATTGTTGGAGGAATCAATCAAGGCACCAAAACAAAAAGCCAAAAAATCGCGTGAACAAAATCTAAATCGAAAAGTAAATGAAGCGGGAATCAACAATGAAGAAAACGAAGAGAATGAAGAAAAAAGGGAGGAAGAAGAAAATGAACAAGAAAAAAGAGAAAACATGCTTCCTATCAAAAAGCCAAATTCAAGAAGAAAGGGAATACCTCGTAGGGCAACCTTTTTTTAA

>MsG0280010452.01.T01

ATGATGTTCGTGCCCTGGCTCAAAAATTTGCTTGCAATCACGGCCTTCTTCACATCATGTGAAGTTCATCTGAATGAGTCAAAAAATGAACGCAACATGTTCTGTCTCGAATGCAATGACAATCCATTTTGTGGATCTTGCATAAAATCTCATCACAAAGATCATCGAGTAATCCAAGTGAGGAGATCATCTTATACAAACGTCATTAAGACAATCGAAATTTACAAACATTTGGATATTCTAGGAATCCAAACCTATGTGATTAGCAACTTTACAGTTGTATTCATAAACAAGAGGCCTTATTCCCAACCTACGAAGAAGACGATAGGTATAATTGGTTGCAGTAGCGACTATCTTTGTAAGATATGCCAAAGGAATCTTGTAGGACCTTATTACTTTTGCTCTCTGGCATGCAAGTTTGAATGCATAAAGAAGGATAGGGGCTTTTTCCTAAGTGCAAAGGAGACAGAAGAAATGGAAAGATTGTTGGAGGAATCAATCAAGGCACCAAAACAAAAAGCCAAAAAATCGCGTGAACAAAATCTAAATCGAAAAGTAAATGAAGCGGGAATCAACAATGAAGAAAACGAAGAGAATGAAGAAAAAAGGGAGGAAGAAGAAAATGAACAAGAAAAAAGAGAAAACATGCTTCCTATCAAAAAGCCAAATTCAAGAAGAAAGGGAATACCTCGTAGGGCAACCTTTTTTTAA

>MsG0280010452.01.T05

ATGTTCTGTCTCGAATGCAATGACAATCCATTTTGTGGATCTTGCATAAAATCTCATCACAAAGATCATCGAGTAATCCAAGTGAGGAGATCATCTTATACAAACGTCATTAAGACAATCGAAATTTACAAACATTTGGATATTCTAGGAATCCAAACCTATGTGATTAGCAACTTTACAGTTGTATTCATAAACAAGAGGCCTTATTCCCAACCTACGAAGAAGACGATAGGTATAATTGGTTGCAGTAGCGACTATCTTTGTAAGATATGCCAAAGGAATCTTGTAGGACCTTATTACTTTTGCTCTCTGGCATGCAAGGTAAATTGTTTTCTCGATCTCATTTTCTTTTTCCTTATCATGTCTCCTTTTTTATAG
Protein sequence

>MsG0280010452.01.T04

MMFVPWLKNLLAITAFFTSCEVHLNESKNERNMFCLECNDNPFCGSCIKSHHKDHRVIQVRRSSYTNVIKTIEIYKHLDILGIQTYVISNFTVVFINKRPYSQPTKKTIGIIGCSSDYLCKICQRNLVGPYYFCSLACKVNCFLDLIFFFLIMSPFL*

>MsG0280010452.01.T03

ITNQVRRSSYTNVIKTIEIYKHLDILGIQTYVISNFTVVFINKRPYSQPTKKTIGIIGCSSDYLCKICQRNLVGPYYFCSLACKFECIKKDRGFFLSAKETEEMERLLEESIKAPKQKAKKSREQNLNRKVNEAGINNEENEENEEKREEEENEQEKRENMLPIKKPNSRRKGIPRRATFF*

>MsG0280010452.01.T02

MFCLECNDNPFCGSCIKSHHKDHRVIQVRRSSYTNVIKTIEIYKHLDILGIQTYVISNFTVVFINKRPYSQPTKKTIGIIGCSAKETEEMERLLEESIKAPKQKAKKSREQNLNRKVNEAGINNEENEENEEKREEEENEQEKRENMLPIKKPNSRRKGIPRRATFF*

>MsG0280010452.01.T01

MMFVPWLKNLLAITAFFTSCEVHLNESKNERNMFCLECNDNPFCGSCIKSHHKDHRVIQVRRSSYTNVIKTIEIYKHLDILGIQTYVISNFTVVFINKRPYSQPTKKTIGIIGCSSDYLCKICQRNLVGPYYFCSLACKFECIKKDRGFFLSAKETEEMERLLEESIKAPKQKAKKSREQNLNRKVNEAGINNEENEENEEKREEEENEQEKRENMLPIKKPNSRRKGIPRRATFF*

>MsG0280010452.01.T05

MFCLECNDNPFCGSCIKSHHKDHRVIQVRRSSYTNVIKTIEIYKHLDILGIQTYVISNFTVVFINKRPYSQPTKKTIGIIGCSSDYLCKICQRNLVGPYYFCSLACKVNCFLDLIFFFLIMSPFL*