AlfalfaGEDB | MsG0280010593.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010593.01

KEGG: K09422
GO: GO:0003677
PFAM: PF00249

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010593.01.T01	QSD99600.1	86.071	481	58	2	1	472	1	481	0	773

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010593.01.T01	O80883	83.594	128	21	0	34	161	17	144	5.08E-72	239

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010593.01.T01	A0A896WBX0	86.071	481	58	2	1	472	1	481	0.0	773

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TFs/TRs:

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Gene ID	Type	Classification
MsG0280010593.01.T01	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010593.01.T01	MTR_2g088170	86.848	479	36	5	1	472	1	459	0.0	751
MsG0280010593.01.T01	MTR_8g042410	64.103	156	52	2	1	155	5	157	5.85e-64	216
MsG0280010593.01.T01	MTR_3g011610	71.654	127	36	0	29	155	31	157	3.95e-63	213
MsG0280010593.01.T01	MTR_3g052450	82.143	112	20	0	35	146	40	151	4.99e-62	211
MsG0280010593.01.T01	MTR_3g052430	81.250	112	21	0	35	146	41	152	5.20e-62	211
MsG0280010593.01.T01	MTR_1g021230	68.033	122	39	0	35	156	57	178	8.43e-56	192
MsG0280010593.01.T01	MTR_1g100653	63.636	110	40	0	34	143	11	120	2.18e-48	168
MsG0280010593.01.T01	MTR_1g083630	66.071	112	38	0	35	146	12	123	2.97e-47	173
MsG0280010593.01.T01	MTR_7g076740	63.063	111	40	1	32	141	9	119	6.31e-47	164
MsG0280010593.01.T01	MTR_2g089620	61.062	113	43	1	32	143	9	121	2.58e-46	164

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010593.01.T01	AT2G32460	83.594	128	21	34	161	5	132	4.83e-73	238
MsG0280010593.01.T01	AT2G32460	83.594	128	21	34	161	17	144	5.18e-73	239
MsG0280010593.01.T01	AT5G06100	77.049	122	28	34	155	31	152	9.78e-68	224
MsG0280010593.01.T01	AT5G06100	75.200	125	31	34	158	31	155	8.39e-67	223
MsG0280010593.01.T01	AT5G06100	75.200	125	31	34	158	31	155	8.39e-67	223
MsG0280010593.01.T01	AT5G06100	75.200	125	31	34	158	31	155	8.39e-67	223
MsG0280010593.01.T01	AT5G06100	75.200	125	31	34	158	31	155	8.39e-67	223
MsG0280010593.01.T01	AT3G11440	72.093	129	36	32	160	38	166	8.42e-66	219
MsG0280010593.01.T01	AT3G11440	72.093	129	36	32	160	38	166	7.36e-65	219
MsG0280010593.01.T01	AT3G11440	72.093	129	36	32	160	38	166	7.36e-65	219

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Find 111 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATATTTGATGATGAAGA+TGG	0.472018	2:-71954190	None:intergenic
AAAGAGGTAAGCCGGCTCTC+TGG	0.465512	2:-71953880	None:intergenic
AACAGGGAAGAAACCAGTCC+AGG	0.517670	2:+71955515	MsG0280010593.01.T01:intron
AAGATATGTTGAAAAGGAGA+AGG	0.524521	2:-71952366	None:intergenic
AAGTATTTCTTGCAATGATA+AGG	0.422893	2:+71954462	MsG0280010593.01.T01:CDS
AATATATGTAAAAGGGAACA+AGG	0.643674	2:-71952241	None:intergenic
AATATTCATAGTGAAGTAGA+TGG	0.493019	2:+71955822	MsG0280010593.01.T01:three_prime_UTR
AATTCAGGGTTGTTAAGGTG+TGG	0.555944	2:+71952796	MsG0280010593.01.T01:CDS
AATTCTGTTCAGAAGAATTC+AGG	0.273502	2:+71952781	MsG0280010593.01.T01:CDS
AATTTGAGCTATGATCATCA+TGG	0.543232	2:+71954265	MsG0280010593.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACTCAATTGATGCTGAAA+AGG	-	Chr2:71953421-71953440	None:intergenic	30.0%
AAATGAAAAAGAGACAGAGA+AGG	-	Chr2:71952287-71952306	None:intergenic	30.0%
AAGATATGTTGAAAAGGAGA+AGG	-	Chr2:71952369-71952388	None:intergenic	30.0%
AATTCTGTTCAGAAGAATTC+AGG	+	Chr2:71952781-71952800	MsG0280010593.01.T01:CDS	30.0%
AATTTGAGCTATGATCATCA+TGG	+	Chr2:71954265-71954284	MsG0280010593.01.T01:CDS	30.0%
AGTGAGGATTCAACATATAA+GGG	+	Chr2:71954490-71954509	MsG0280010593.01.T01:CDS	30.0%
ATCTCATAAAAGGAAAAGCA+TGG	+	Chr2:71954519-71954538	MsG0280010593.01.T01:CDS	30.0%
ATTCTGTTCAGAAGAATTCA+GGG	+	Chr2:71952782-71952801	MsG0280010593.01.T01:CDS	30.0%
ATTTATTATTGGAGCAAAGG+TGG	+	Chr2:71952568-71952587	MsG0280010593.01.T01:five_prime_UTR	30.0%
GAAGATATGTTGAAAAAGAG+AGG	-	Chr2:71952320-71952339	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	71952212	71955954	71952212	ID=MsG0280010593.01;Name=MsG0280010593.01
Chr2	mRNA	71952212	71955954	71952212	ID=MsG0280010593.01.T01;Parent=MsG0280010593.01;Name=MsG0280010593.01.T01;_AED=0.25;_eAED=0.25;_QI=389\|1\|1\|1\|1\|1\|3\|190\|472
Chr2	exon	71952212	71952954	71952212	ID=MsG0280010593.01.T01:exon:19485;Parent=MsG0280010593.01.T01
Chr2	exon	71953821	71954640	71953821	ID=MsG0280010593.01.T01:exon:19486;Parent=MsG0280010593.01.T01
Chr2	exon	71955520	71955954	71955520	ID=MsG0280010593.01.T01:exon:19487;Parent=MsG0280010593.01.T01
Chr2	five_prime_UTR	71952212	71952600	71952212	ID=MsG0280010593.01.T01:five_prime_utr;Parent=MsG0280010593.01.T01
Chr2	CDS	71952601	71952954	71952601	ID=MsG0280010593.01.T01:cds;Parent=MsG0280010593.01.T01
Chr2	CDS	71953821	71954640	71953821	ID=MsG0280010593.01.T01:cds;Parent=MsG0280010593.01.T01
Chr2	CDS	71955520	71955764	71955520	ID=MsG0280010593.01.T01:cds;Parent=MsG0280010593.01.T01
Chr2	three_prime_UTR	71955765	71955954	71955765	ID=MsG0280010593.01.T01:three_prime_utr;Parent=MsG0280010593.01.T01

Gene Sequence

>MsG0280010593.01.T01

ATGGCGAAAACGATCTCGAACATCAAAATCAACGACAACAACAATAATAATGTTGAGGTTGACATTGACTCGTCAAAAGATGATGTTGATGCTAGAACTGGCATGAAGAAGGGACCGTGGACCCCACATGAAGACATGATATTGACAGAGTACGTGAATAAGCATGGTGAAGGGAATTGGAATTCTGTTCAGAAGAATTCAGGGTTGTTAAGGTGTGGAAAAAGCTGCAGACTTAGATGGGCGAATCATTTGAGGCCAAATCTTAAAAAGGGTTCATTTTCTGAAGAGGAAGAAAAAATCATCATTGAACTTCATGCTAAGCTTGGAAACAAATGGGCTCGAATGGCTACTCAGTTGCCCGGAAGAACGGATAACGAAATCAAAAACTTTTGGAACACGAGAATGAAAAGGCGCCAGAGAGCCGGCTTACCTCTTTATCCTCCAGAAATCCAAGCTGAAGCTATTGTATACAATGATAAACTATTGCAACTACAACATGAACCTTTATCTTCTTCATCATTATCATTTTCTTTACTCTTATCTTCTTGTTACCCTAAGAAGCTTGATGATCCAAACCATTATGATTATAATCCTCCACAAAACAACTCTGATTCTTCTAATCACTACACCAATCCATGTCCACAATTCAACTTTTCCAATGAAGAAAACATTGAGATTAATGAAACTCTTGCTTTGCAAAAATCACCTTCACTTTCACCTTATCCATCTTCATCATCAAATATTTTCAACCAAAATTTTACTCCTCCAAGTGATTCACATGATTACCAATATTCTGAAAATTTGAGCTATGATCATCATGGATTCAATGCAGGATCTCTGTATGATTCTGAGACTCCTGCTTCATCTTATGCTAGTGGTGTTGATGGTAATTTTATGGGAAATTCTAGCATGGTTAATGATTACTATGAAGTTGCACCATTATCCCATGAAGGAAAAAATAGTGGACTGTTGGATGATCTTGTGATGGAAGGTAGAAGTATTTCTTGCAATGATAAGGCTAAGAGTGAGGATTCAACATATAAGGGGAGTGTATCTCATAAAAGGAAAAGCATGGAGGTTGAAGAATATGCAGAGGAAGAAAGTTTTTCCATGAAGAAGAAGAATATTGATGCGATTCAGAAGAAAGATTTCAGCACTTCTGACTTGTCAACAGGGAAGAAACCAGTCCAGGAGGATGAGGTAGAAGAGCTGAATACAATGTACGATGATGATTTGGATTGCTTGCTCAACAATTTTCCCTCAGAAATACCAATGCCTGAATGGTATTGTAGAGGAAAAAGTCAGTCATTGGGAACTGAAACTCCAACAGATGAAGCTTCTTCATCAGGTGGTCCCACCAATCAAGAATTTGCTTGGACTCTTGGATCAAATTGGAACAACATGCCTGGCATATGTTAA

Protein sequence

>MsG0280010593.01.T01

MAKTISNIKINDNNNNNVEVDIDSSKDDVDARTGMKKGPWTPHEDMILTEYVNKHGEGNWNSVQKNSGLLRCGKSCRLRWANHLRPNLKKGSFSEEEEKIIIELHAKLGNKWARMATQLPGRTDNEIKNFWNTRMKRRQRAGLPLYPPEIQAEAIVYNDKLLQLQHEPLSSSSLSFSLLLSSCYPKKLDDPNHYDYNPPQNNSDSSNHYTNPCPQFNFSNEENIEINETLALQKSPSLSPYPSSSSNIFNQNFTPPSDSHDYQYSENLSYDHHGFNAGSLYDSETPASSYASGVDGNFMGNSSMVNDYYEVAPLSHEGKNSGLLDDLVMEGRSISCNDKAKSEDSTYKGSVSHKRKSMEVEEYAEEESFSMKKKNIDAIQKKDFSTSDLSTGKKPVQEDEVEELNTMYDDDLDCLLNNFPSEIPMPEWYCRGKSQSLGTETPTDEASSSGGPTNQEFAWTLGSNWNNMPGIC*