AlfalfaGEDB | MsG0280010653.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010653.01

KEGG: K09422
GO: GO:0003677
PFAM: PF00249

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010653.01.T01	QSD99623.1	86.301	365	11	2	1	362	1	329	0	601

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010653.01.T01	Q9LDR8	41.951	410	129	13	1	362	1	349	5.47E-86	266

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010653.01.T01	A0A896WC70	86.301	365	11	2	1	362	1	329	0.0	601

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TFs/TRs:

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Gene ID	Type	Classification
MsG0280010653.01.T01	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010653.01.T01	MTR_2g089620	88.398	362	6	1	1	362	1	326	0.0	597
MsG0280010653.01.T01	MTR_4g091490	43.358	399	148	13	1	362	1	358	1.25e-84	261
MsG0280010653.01.T01	MTR_2g011660	69.277	166	14	2	1	165	1	130	3.35e-75	236
MsG0280010653.01.T01	MTR_4g478180	57.459	181	41	1	1	181	2	146	7.14e-69	220
MsG0280010653.01.T01	MTR_5g029840	53.425	219	56	3	1	219	1	173	1.45e-67	216
MsG0280010653.01.T01	MTR_6g012690	57.803	173	37	1	1	173	1	137	7.61e-66	213
MsG0280010653.01.T01	MTR_7g011170	57.059	170	37	1	1	170	1	134	3.68e-65	209
MsG0280010653.01.T01	MTR_4g082290	59.524	168	32	1	1	168	1	132	4.77e-65	212
MsG0280010653.01.T01	MTR_3g074520	57.485	167	35	1	1	167	1	131	1.56e-64	209
MsG0280010653.01.T01	MTR_4g082230	57.738	168	35	1	1	168	1	132	8.33e-62	203

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010653.01.T01	AT4G21440	41.951	410	129	13	1	362	1	349	5.58e-87	266
MsG0280010653.01.T01	AT4G05100	43.164	373	149	8	1	362	1	321	7.73e-82	253
MsG0280010653.01.T01	AT4G28110	68.485	165	16	1	1	165	1	129	1.08e-77	240
MsG0280010653.01.T01	AT5G16770	58.011	181	40	1	1	181	1	145	9.57e-69	219
MsG0280010653.01.T01	AT5G16770	58.011	181	40	1	1	181	1	145	9.57e-69	219
MsG0280010653.01.T01	AT3G02940	61.538	169	29	1	1	169	1	133	9.88e-69	218
MsG0280010653.01.T01	AT5G16770	58.011	181	40	1	1	181	1	145	2.14e-68	218
MsG0280010653.01.T01	AT5G16770	58.011	181	40	1	1	181	1	145	2.14e-68	218
MsG0280010653.01.T01	AT3G02940	61.538	169	29	1	1	169	1	133	2.61e-68	218
MsG0280010653.01.T01	AT3G02940	61.538	169	29	1	1	169	1	133	2.61e-68	218

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Find 70 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAGTATGGGATATGAAT+CGG	0.505907	2:-72980839	MsG0280010653.01.T01:CDS
AAATCCTCTCCAAAATAACA+AGG	0.633825	2:+72980916	None:intergenic
AAATGATGGGTTCATTGGGT+TGG	0.498615	2:+72981200	None:intergenic
AACAGGGAAGAAGAAGAAGA+TGG	0.550626	2:+72981942	None:intergenic
AACCTTCCTCTCTTGATATC+AGG	0.347637	2:+72982989	None:intergenic
AATACAAATCCTTGTTATTT+TGG	0.086118	2:-72980925	MsG0280010653.01.T01:CDS
AATACTCTCTTCTTCTTCAA+AGG	0.469466	2:+72982964	None:intergenic
AATGTGAATAATGGCTTATT+AGG	0.258840	2:-72980976	MsG0280010653.01.T01:CDS
ACAATGAAATAAAGAACTAC+TGG	0.460692	2:-72981347	MsG0280010653.01.T01:CDS
ACACCTTGTTGTGATAAAAG+TGG	0.533939	2:-72983262	MsG0280010653.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACAGTATGGGATATGAAT+CGG	-	Chr2:72983122-72983141	MsG0280010653.01.T01:intron	30.0%
AAATCCTCTCCAAAATAACA+AGG	+	Chr2:72983048-72983067	None:intergenic	30.0%
AATACTCTCTTCTTCTTCAA+AGG	+	Chr2:72981000-72981019	None:intergenic	30.0%
ACATTCTACACAATAGACTA+AGG	+	Chr2:72982387-72982406	None:intergenic	30.0%
ACCATGAACATGAACATTAT+AGG	-	Chr2:72982554-72982573	MsG0280010653.01.T01:intron	30.0%
ACCTATAATGTTCATGTTCA+TGG	+	Chr2:72982558-72982577	None:intergenic	30.0%
AGCTGATCTGAGAATATTAA+TGG	+	Chr2:72982728-72982747	None:intergenic	30.0%
ATAAGCCATTATTCACATTG+TGG	+	Chr2:72982984-72983003	None:intergenic	30.0%
ATGAACATGAACATTATAGG+TGG	-	Chr2:72982557-72982576	MsG0280010653.01.T01:intron	30.0%
CAAATCAATGATGTTGCTAA+TGG	-	Chr2:72982895-72982914	MsG0280010653.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	72980690	72983293	72980690	ID=MsG0280010653.01;Name=MsG0280010653.01
Chr2	mRNA	72980690	72983293	72980690	ID=MsG0280010653.01.T01;Parent=MsG0280010653.01;Name=MsG0280010653.01.T01;_AED=0.22;_eAED=0.29;_QI=0\|0\|0\|1\|1\|1\|4\|0\|362
Chr2	exon	72983161	72983293	72983161	ID=MsG0280010653.01.T01:exon:15389;Parent=MsG0280010653.01.T01
Chr2	exon	72982935	72983064	72982935	ID=MsG0280010653.01.T01:exon:15388;Parent=MsG0280010653.01.T01
Chr2	exon	72981903	72982010	72981903	ID=MsG0280010653.01.T01:exon:15387;Parent=MsG0280010653.01.T01
Chr2	exon	72980690	72981407	72980690	ID=MsG0280010653.01.T01:exon:15386;Parent=MsG0280010653.01.T01
Chr2	CDS	72983161	72983293	72983161	ID=MsG0280010653.01.T01:cds;Parent=MsG0280010653.01.T01
Chr2	CDS	72982935	72983064	72982935	ID=MsG0280010653.01.T01:cds;Parent=MsG0280010653.01.T01
Chr2	CDS	72981903	72982010	72981903	ID=MsG0280010653.01.T01:cds;Parent=MsG0280010653.01.T01
Chr2	CDS	72980690	72981407	72980690	ID=MsG0280010653.01.T01:cds;Parent=MsG0280010653.01.T01

Gene Sequence

>MsG0280010653.01.T01

ATGGGAAGAACACCTTGTTGTGATAAAAGTGGACTCAAAAAAGGTCCATGGACACAAGAAGAAGATCTCATGCTTATCAACTACATTAATACTCATGGACCTGGAAATTGGCGCAACCTCCCCAAAAATGCTGGTCTTCAAAGGTGTGGCAAGAGTTGTCGCCTTCGATGGACAAACTATTTGAGACCTGATATCAAGAGAGGAAGGTTCTCCTTTGAAGAAGAAGAGAGTATTATTCAGCTCCACAGTGTCTTGGGAAACAAAGCCAAAACCGCTTCATCTTTCCCTTCCCCTGTTTTCAAAACCGCTCCATCTTCTTCTTCTTCCCTGTTTTCTCTTTCTGGTGGCTGCTCCGATCTTCTTCCTCACAGGTGGTCTGCTATAGCTGCTAGATTACCCGGAAGAACAGACAATGAAATAAAGAACTACTGGAACACACACATTAGGAAAAGACTACTTCGAAGTGGAATTGATCCTGTTACGCATACTCCACGTCTCGATCATCTATTTGACATGTCCTCCATTAATATTCTCAGATCAGCTTTATTAGGAGGAACCAACCCAATGAACCCATCATTTCTGAACTTATTAGGTGGTGCTCAAGCTTTGATGAACCCTGAATTGTTAAAGCTAGCTGCAACGGCGACATTATTAAACAACAACATAAATTTGGGTTCACAGAATCAACAACAAATCAATGATGTTGCTAATGGTACTCAACAACAAGTGCAAAATCAAGTTTACCCTCAAGATCAATTTCAAATACCAACTCAAACCCACAATGTGAATAATGGCTTATTAGGAAACATGAGTTGTTCAAGTTCAGCTGAAAATACAAATCCTTGTTATTTTGGAGAGGATTTGATTTCTAAACAATATTCAGAATTGTTTCATTGCCTAAATAATGATGGAAACAAAAACAGTATGGGATATGAATCGGTTATATCGACTACTCCTTTATCGACGACACCAACACCATCATCAACATATGTGAATAGCTGCACAGAGGAAGAGAGGGATACATATTGCAGTGACATATTCAAGTTCGAAATTCCAGAGAGTACTTTGGACATAAATGATTTCTTGTAA

Protein sequence

>MsG0280010653.01.T01

MGRTPCCDKSGLKKGPWTQEEDLMLINYINTHGPGNWRNLPKNAGLQRCGKSCRLRWTNYLRPDIKRGRFSFEEEESIIQLHSVLGNKAKTASSFPSPVFKTAPSSSSSLFSLSGGCSDLLPHRWSAIAARLPGRTDNEIKNYWNTHIRKRLLRSGIDPVTHTPRLDHLFDMSSINILRSALLGGTNPMNPSFLNLLGGAQALMNPELLKLAATATLLNNNINLGSQNQQQINDVANGTQQQVQNQVYPQDQFQIPTQTHNVNNGLLGNMSCSSSAENTNPCYFGEDLISKQYSELFHCLNNDGNKNSMGYESVISTTPLSTTPTPSSTYVNSCTEEERDTYCSDIFKFEIPESTLDINDFL*