AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010978.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010978.01.T01 MTR_2g096080 80.786 229 24 4 1 225 1 213 4.95e-111 319
MsG0280010978.01.T01 MTR_4g061823 53.846 195 70 4 33 226 29 204 1.06e-57 184
MsG0280010978.01.T01 MTR_4g061910 53.846 195 70 4 33 226 29 204 1.06e-57 184
MsG0280010978.01.T01 MTR_8g098725 39.412 170 79 7 55 218 132 283 3.58e-21 90.5
MsG0280010978.01.T01 MTR_1g044785 35.897 156 80 5 90 237 55 198 4.11e-21 88.6
MsG0280010978.01.T01 MTR_8g098725 58.667 75 28 1 147 218 205 279 2.56e-20 88.2
MsG0280010978.01.T01 MTR_2g068730 57.692 78 28 1 158 235 154 226 6.23e-20 86.3
MsG0280010978.01.T01 MTR_1g073350 56.716 67 29 0 145 211 294 360 1.87e-18 84.0
MsG0280010978.01.T01 MTR_5g010120 55.556 72 32 0 145 216 151 222 1.12e-17 79.7
MsG0280010978.01.T01 MTR_3g091340 57.692 52 22 0 163 214 197 248 4.85e-15 72.8
MsG0280010978.01.T01 MTR_3g091340 57.692 52 22 0 163 214 190 241 5.50e-15 72.4
MsG0280010978.01.T01 MTR_1g008220 54.717 53 24 0 163 215 228 280 6.13e-15 72.8
MsG0280010978.01.T01 MTR_3g091340 57.692 52 22 0 163 214 226 277 7.84e-15 72.4
MsG0280010978.01.T01 MTR_1g013450 36.145 83 40 1 156 238 244 313 5.98e-11 61.6
MsG0280010978.01.T01 MTR_4g128930 44.262 61 31 1 153 213 287 344 9.95e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010978.01.T01 AT5G59990 70.130 77 23 0 143 219 139 215 2.73e-24 97.4
MsG0280010978.01.T01 AT5G41380 39.080 174 78 6 68 230 128 284 1.56e-23 96.7
MsG0280010978.01.T01 AT1G63820 46.847 111 47 2 143 241 163 273 5.78e-23 95.1
MsG0280010978.01.T01 AT3G12890 38.596 171 76 6 88 231 36 204 6.09e-22 91.3
MsG0280010978.01.T01 AT1G04500 48.315 89 41 1 129 212 245 333 1.31e-21 92.8
MsG0280010978.01.T01 AT1G04500 48.315 89 41 1 129 212 245 333 1.31e-21 92.8
MsG0280010978.01.T01 AT1G04500 48.315 89 41 1 129 212 236 324 1.31e-21 92.4
MsG0280010978.01.T01 AT2G33350 50.000 90 38 2 129 211 254 343 1.93e-21 92.4
MsG0280010978.01.T01 AT2G33350 45.714 105 46 3 118 211 250 354 2.12e-21 92.4
MsG0280010978.01.T01 AT2G33350 50.000 90 38 2 129 211 274 363 2.36e-21 92.0
MsG0280010978.01.T01 AT2G33350 46.809 94 43 2 125 211 262 355 7.23e-21 90.9
MsG0280010978.01.T01 AT4G27900 53.846 52 24 0 163 214 210 261 3.19e-14 70.5
MsG0280010978.01.T01 AT4G27900 53.846 52 24 0 163 214 210 261 3.19e-14 70.5
MsG0280010978.01.T01 AT5G53420 28.108 185 118 4 27 211 14 183 2.90e-13 66.6
MsG0280010978.01.T01 AT5G53420 28.108 185 118 4 27 211 14 183 2.90e-13 66.6
MsG0280010978.01.T01 AT5G53420 28.108 185 118 4 27 211 14 183 2.90e-13 66.6
MsG0280010978.01.T01 AT5G53420 28.108 185 118 4 27 211 93 262 3.53e-13 67.8
MsG0280010978.01.T01 AT2G24790 43.056 72 37 1 141 208 199 270 5.30e-11 61.6
MsG0280010978.01.T01 AT5G15850 45.283 53 29 0 163 215 282 334 6.54e-11 62.0

Find 65 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCATCTCCTTCTCCATTTA+TGG 0.057189 2:+77864124 MsG0280010978.01.T01:CDS
ATTCTCACTTTCTTCTCTTC+TGG 0.076177 2:-77864915 None:intergenic
CGGATAGGAGGCCACGAATT+AGG 0.208419 2:+77865962 MsG0280010978.01.T01:CDS
TTCTCACTTTCTTCTCTTCT+GGG 0.251110 2:-77864914 None:intergenic
TTCTTCTCTTCTGGGCTATA+TGG 0.254110 2:-77864906 None:intergenic
GAACTTTAACAAGAAAATTA+AGG 0.269338 2:+77864968 MsG0280010978.01.T01:CDS
GGATAGGAGGCCACGAATTA+GGG 0.287165 2:+77865963 MsG0280010978.01.T01:CDS
ACGTACAATATACTTTAAAA+AGG 0.308104 2:-77866212 None:intergenic
AACAAGATTTGCAGCAACTA+TGG 0.316149 2:-77866099 None:intergenic
GAGGATGAAGAAGATGAAAT+TGG 0.316625 2:+77866060 MsG0280010978.01.T01:CDS
CATTCCCTTCAGTACAATAA+TGG 0.321357 2:+77864293 MsG0280010978.01.T01:CDS
TCAGGGATATGTTCCATAAA+TGG 0.329934 2:-77864137 None:intergenic
TTGCAGTACCGGCGATTTGC+AGG 0.339328 2:+77864386 MsG0280010978.01.T01:CDS
TTTCAAGGAAGTTCTTCCTT+TGG 0.354729 2:+77866131 MsG0280010978.01.T01:three_prime_UTR
GAGTCTAACTCATTGAAATC+AGG 0.374546 2:-77864155 None:intergenic
CTTGTTCATGATGAGTTTCA+AGG 0.385277 2:+77866116 MsG0280010978.01.T01:three_prime_UTR
TCCTATCCGCCAATGTCTTT+CGG 0.395899 2:-77865948 None:intergenic
TGTATTATTGAATCAAACTT+TGG 0.409644 2:+77866387 MsG0280010978.01.T01:three_prime_UTR
ATGTGGCTCCACTCGACTAT+AGG 0.414777 2:-77866021 None:intergenic
GAAAGTAGTATGATCATAGA+AGG 0.422699 2:+77864867 MsG0280010978.01.T01:CDS
ATTCCCTTCAGTACAATAAT+GGG 0.429789 2:+77864294 MsG0280010978.01.T01:CDS
TGCATCAAGATTGGAGTACT+TGG 0.434559 2:-77864254 None:intergenic
ATGCTCCTCTGCATCAAGAT+TGG 0.438379 2:-77864263 None:intergenic
CATTGGTGGTGGGGAGGAAG+AGG 0.451326 2:+77866041 MsG0280010978.01.T01:CDS
GAAGGAGATGGAAGAAGAAA+GGG 0.457613 2:-77864113 None:intergenic
AGAAGGAGATGGAAGAAGAA+AGG 0.460726 2:-77864114 None:intergenic
AGAAGAAAGGGTGTTGAGAA+TGG 0.469563 2:-77864101 None:intergenic
GAGTGGAGCCACATTGGTGG+TGG 0.477837 2:+77866030 MsG0280010978.01.T01:CDS
TTGAAAGATACAGAAGAAAG+AGG 0.482706 2:+77864937 MsG0280010978.01.T01:CDS
GTGAGGAAAGTTTGCAGTAC+CGG 0.487222 2:+77864375 MsG0280010978.01.T01:CDS
TGTTGAGAATGGAATATTGT+TGG 0.508851 2:-77864090 None:intergenic
AGTGGAGCCACATTGGTGGT+GGG 0.516680 2:+77866031 MsG0280010978.01.T01:CDS
GCAAAACGTCCCCTAATTCG+TGG 0.530988 2:-77865973 None:intergenic
AGCAGTGGCTATAGTAGCTA+TGG 0.535103 2:+77864218 MsG0280010978.01.T01:CDS
AGTCTAACTCATTGAAATCA+GGG 0.536173 2:-77864154 None:intergenic
GAGTCCCATTATTGTACTGA+AGG 0.542232 2:-77864298 None:intergenic
GTCGAGTGGAGCCACATTGG+TGG 0.548825 2:+77866027 MsG0280010978.01.T01:CDS
GCCGAAAGACATTGGCGGAT+AGG 0.550818 2:+77865947 MsG0280010978.01.T01:CDS
ACAGAAGAAAGAGGAATCAG+AGG 0.553930 2:+77864946 MsG0280010978.01.T01:CDS
GTATGTTTGCCGAAAGACAT+TGG 0.562441 2:+77865939 MsG0280010978.01.T01:intron
GAGCCACATTGGTGGTGGGG+AGG 0.566927 2:+77866035 MsG0280010978.01.T01:CDS
GAAAGACATTGGCGGATAGG+AGG 0.570627 2:+77865950 MsG0280010978.01.T01:CDS
TCCATAAATGGAGAAGGAGA+TGG 0.571408 2:-77864125 None:intergenic
GTGGAGCCACATTGGTGGTG+GGG 0.572282 2:+77866032 MsG0280010978.01.T01:CDS
TCAACTCCGAGTTATTCAGC+TGG 0.581544 2:-77864196 None:intergenic
TCAGCTGGGAGAGAGTGTCT+TGG 0.586821 2:-77864181 None:intergenic
GTACTCCAATCTTGATGCAG+AGG 0.591070 2:+77864258 MsG0280010978.01.T01:CDS
ATACTACTTTCACTAGACAA+TGG 0.602525 2:-77864855 None:intergenic
AAGATTGGAGTACTTGGAGA+AGG 0.605160 2:-77864248 None:intergenic
CAACTCCGAGTTATTCAGCT+GGG 0.608456 2:-77864195 None:intergenic
CTTCCTCCCCACCACCAATG+TGG 0.613096 2:-77866038 None:intergenic
ATATGTTCCATAAATGGAGA+AGG 0.614198 2:-77864131 None:intergenic
AGTCCCATTATTGTACTGAA+GGG 0.624751 2:-77864297 None:intergenic
ATTCTAATATAATAGACCAA+AGG 0.637951 2:-77866147 None:intergenic
GATAGGAGGCCACGAATTAG+GGG 0.642140 2:+77865964 MsG0280010978.01.T01:CDS
ATAGTGTACCTGCAAATCGC+CGG 0.645719 2:-77864394 None:intergenic
AGTGGCTATAGTAGCTATGG+TGG 0.656066 2:+77864221 MsG0280010978.01.T01:CDS
ATAGTCGAGTGGAGCCACAT+TGG 0.657395 2:+77866024 MsG0280010978.01.T01:CDS
TCTCTCCCAGCTGAATAACT+CGG 0.662391 2:+77864190 MsG0280010978.01.T01:CDS
ATAAGAAACCTATAGTCGAG+TGG 0.670337 2:+77866013 MsG0280010978.01.T01:CDS
GGTACTGCAAACTTTCCTCA+CGG 0.677734 2:-77864373 None:intergenic
AATAACTCGGAGTTGAGCAG+TGG 0.681830 2:+77864203 MsG0280010978.01.T01:CDS
GACTCAGACGATGCTCCGTG+AGG 0.691893 2:+77864358 MsG0280010978.01.T01:CDS
TGTTTGCCGAAAGACATTGG+CGG 0.695673 2:+77865942 MsG0280010978.01.T01:CDS
GCAACATAATCATCATCACG+TGG 0.724747 2:+77864827 MsG0280010978.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTAAGAATGTTTTTTTTA+GGG + Chr2:77864742-77864761 MsG0280010978.01.T01:intron 10.0%
!!! TATTTAAGAATGTTTTTTTT+AGG + Chr2:77864741-77864760 MsG0280010978.01.T01:intron 10.0%
!!! CGTTAAACTTTTTTTTTTTT+TGG + Chr2:77864479-77864498 MsG0280010978.01.T01:intron 15.0%
!!! GAAAAACATTTTTTTTTTTG+CGG - Chr2:77864637-77864656 None:intergenic 15.0%
!!! GAGTATATTGTTATTAATAA+GGG - Chr2:77865585-77865604 None:intergenic 15.0%
!!! TGCAATTAAGTTTTAAATAA+TGG - Chr2:77864542-77864561 None:intergenic 15.0%
!! AGTGATAGAAAATTTCATAA+AGG - Chr2:77865204-77865223 None:intergenic 20.0%
!! ATTAGTGTATGAAAGTTAAA+TGG - Chr2:77865101-77865120 None:intergenic 20.0%
!! ATTCTAATATAATAGACCAA+AGG - Chr2:77866150-77866169 None:intergenic 20.0%
!! GAACTTTAACAAGAAAATTA+AGG + Chr2:77864968-77864987 MsG0280010978.01.T01:CDS 20.0%
!! GTGATAGAAAATTTCATAAA+GGG - Chr2:77865203-77865222 None:intergenic 20.0%
!! TATAAAAAAGAACAGAAGTA+TGG + Chr2:77865137-77865156 MsG0280010978.01.T01:intron 20.0%
!! TGATAGAAAATTTCATAAAG+GGG - Chr2:77865202-77865221 None:intergenic 20.0%
!! TGGTGAAAAAATAAATTCAT+TGG + Chr2:77865277-77865296 MsG0280010978.01.T01:intron 20.0%
!! TGTATTATTGAATCAAACTT+TGG + Chr2:77866387-77866406 MsG0280010978.01.T01:three_prime_UTR 20.0%
!!! AACAGTATACATTTTTTTGA+AGG - Chr2:77865640-77865659 None:intergenic 20.0%
!!! ACGTACAATATACTTTAAAA+AGG - Chr2:77866215-77866234 None:intergenic 20.0%
!!! GGAGTATATTGTTATTAATA+AGG - Chr2:77865586-77865605 None:intergenic 20.0%
!!! TCGCATTTTAATAATTTTAG+AGG + Chr2:77864444-77864463 MsG0280010978.01.T01:intron 20.0%
! AAAACTTTGTGCATTTCTTT+AGG - Chr2:77864684-77864703 None:intergenic 25.0%
! AAGTCCTATAAAAAGATACA+GGG - Chr2:77866351-77866370 None:intergenic 25.0%
! AATTTGTACTAAATAGCGTA+TGG - Chr2:77865791-77865810 None:intergenic 25.0%
! AGTACAAATTATTGTCATAG+TGG + Chr2:77865801-77865820 MsG0280010978.01.T01:intron 25.0%
! ATACATGTACACTATGAATT+TGG - Chr2:77865607-77865626 None:intergenic 25.0%
! CCATCATTATACTACTATTT+AGG + Chr2:77865676-77865695 MsG0280010978.01.T01:intron 25.0%
! CCTAAATAGTAGTATAATGA+TGG - Chr2:77865679-77865698 None:intergenic 25.0%
! TAACTAATTACGCATGTATA+AGG - Chr2:77865314-77865333 None:intergenic 25.0%
! TAAGTCCTATAAAAAGATAC+AGG - Chr2:77866352-77866371 None:intergenic 25.0%
!! AACTCTAAAAACTGCATATA+TGG - Chr2:77866304-77866323 None:intergenic 25.0%
!!! ATACTGTTTATTGATTCTAG+TGG + Chr2:77865652-77865671 MsG0280010978.01.T01:intron 25.0%
!!! TGAATTTATTTTTTCACCAG+TGG - Chr2:77865276-77865295 None:intergenic 25.0%
AAAGAGGGAAAATAAATGCA+TGG + Chr2:77865172-77865191 MsG0280010978.01.T01:intron 30.0%
AACTCGATTAGAATATTGAC+CGG + Chr2:77865705-77865724 MsG0280010978.01.T01:intron 30.0%
AGTCTAACTCATTGAAATCA+GGG - Chr2:77864157-77864176 None:intergenic 30.0%
ATACTACTTTCACTAGACAA+TGG - Chr2:77864858-77864877 None:intergenic 30.0%
ATATGTTCCATAAATGGAGA+AGG - Chr2:77864134-77864153 None:intergenic 30.0%
ATTCCCTTCAGTACAATAAT+GGG + Chr2:77864294-77864313 MsG0280010978.01.T01:CDS 30.0%
CTAAAAGACACTTCAAATCA+TGG - Chr2:77865419-77865438 None:intergenic 30.0%
GAAAGTAGTATGATCATAGA+AGG + Chr2:77864867-77864886 MsG0280010978.01.T01:CDS 30.0%
TGTTGAGAATGGAATATTGT+TGG - Chr2:77864093-77864112 None:intergenic 30.0%
TTGAAAGATACAGAAGAAAG+AGG + Chr2:77864937-77864956 MsG0280010978.01.T01:CDS 30.0%
!! AGCGTTATAGCATTTTAGTA+CGG + Chr2:77865831-77865850 MsG0280010978.01.T01:intron 30.0%
!!! ATTGTTTTAGATTGCAGTTG+CGG + Chr2:77864421-77864440 MsG0280010978.01.T01:intron 30.0%
AACAAGATTTGCAGCAACTA+TGG - Chr2:77866102-77866121 None:intergenic 35.0%
AGTCCCATTATTGTACTGAA+GGG - Chr2:77864300-77864319 None:intergenic 35.0%
ATAAGAAACCTATAGTCGAG+TGG + Chr2:77866013-77866032 MsG0280010978.01.T01:CDS 35.0%
ATAGTGTGCTATAACTGGTT+TGG - Chr2:77865736-77865755 None:intergenic 35.0%
ATGGACAAAACTGTACAAAG+AGG + Chr2:77865156-77865175 MsG0280010978.01.T01:intron 35.0%
ATTCTCACTTTCTTCTCTTC+TGG - Chr2:77864918-77864937 None:intergenic 35.0%
CATTCCCTTCAGTACAATAA+TGG + Chr2:77864293-77864312 MsG0280010978.01.T01:CDS 35.0%
CTTGTTCATGATGAGTTTCA+AGG + Chr2:77866116-77866135 MsG0280010978.01.T01:three_prime_UTR 35.0%
GAGGATGAAGAAGATGAAAT+TGG + Chr2:77866060-77866079 MsG0280010978.01.T01:CDS 35.0%
GAGTCTAACTCATTGAAATC+AGG - Chr2:77864158-77864177 None:intergenic 35.0%
TAGTCAGTGTATATAGCAAC+AGG + Chr2:77865436-77865455 MsG0280010978.01.T01:intron 35.0%
TCAGGGATATGTTCCATAAA+TGG - Chr2:77864140-77864159 None:intergenic 35.0%
TGAGGTGTGAAATCTTCTTA+CGG + Chr2:77865471-77865490 MsG0280010978.01.T01:intron 35.0%
TGCTAATAGTGTGCTATAAC+TGG - Chr2:77865741-77865760 None:intergenic 35.0%
TGGACAAAACTGTACAAAGA+GGG + Chr2:77865157-77865176 MsG0280010978.01.T01:intron 35.0%
TTCTCACTTTCTTCTCTTCT+GGG - Chr2:77864917-77864936 None:intergenic 35.0%
TTTCAAGGAAGTTCTTCCTT+TGG + Chr2:77866131-77866150 MsG0280010978.01.T01:three_prime_UTR 35.0%
! AACAGGTTCAGAATTTTGTG+AGG + Chr2:77865453-77865472 MsG0280010978.01.T01:intron 35.0%
! AGTGCCCTGTATCTTTTTAT+AGG + Chr2:77866344-77866363 MsG0280010978.01.T01:three_prime_UTR 35.0%
! CGTTTTTCACAGAGGATTAA+CGG + Chr2:77864801-77864820 MsG0280010978.01.T01:CDS 35.0%
! TTGTTATTAATAAGGGCGTG+TGG - Chr2:77865578-77865597 None:intergenic 35.0%
!! GTTATAGCATTTTAGTACGG+TGG + Chr2:77865834-77865853 MsG0280010978.01.T01:intron 35.0%
!!! AGTTTTTAGAGTTCAGTCAG+GGG + Chr2:77866311-77866330 MsG0280010978.01.T01:three_prime_UTR 35.0%
!!! CAGTTTTTAGAGTTCAGTCA+GGG + Chr2:77866310-77866329 MsG0280010978.01.T01:three_prime_UTR 35.0%
AATTCAGCAAAAAAGCGGAG+AGG - Chr2:77864334-77864353 None:intergenic 40.0%
ACAGAAGAAAGAGGAATCAG+AGG + Chr2:77864946-77864965 MsG0280010978.01.T01:CDS 40.0%
AGAAGGAGATGGAAGAAGAA+AGG - Chr2:77864117-77864136 None:intergenic 40.0%
ATTCAGCAAAAAAGCGGAGA+GGG - Chr2:77864333-77864352 None:intergenic 40.0%
CGAGCAATTCAGCAAAAAAG+CGG - Chr2:77864339-77864358 None:intergenic 40.0%
CTTGGAGAACAGAATCAATC+AGG + Chr2:77865541-77865560 MsG0280010978.01.T01:intron 40.0%
GAAGGAGATGGAAGAAGAAA+GGG - Chr2:77864116-77864135 None:intergenic 40.0%
GAGTCCCATTATTGTACTGA+AGG - Chr2:77864301-77864320 None:intergenic 40.0%
GCAACATAATCATCATCACG+TGG + Chr2:77864827-77864846 MsG0280010978.01.T01:CDS 40.0%
GTATGTTTGCCGAAAGACAT+TGG + Chr2:77865939-77865958 MsG0280010978.01.T01:intron 40.0%
TCCATAAATGGAGAAGGAGA+TGG - Chr2:77864128-77864147 None:intergenic 40.0%
TCCATCTCCTTCTCCATTTA+TGG + Chr2:77864124-77864143 MsG0280010978.01.T01:CDS 40.0%
TGCATTTCTTTAGGTACGCT+TGG - Chr2:77864675-77864694 None:intergenic 40.0%
TTCTTCTCTTCTGGGCTATA+TGG - Chr2:77864909-77864928 None:intergenic 40.0%
! AAGATTGGAGTACTTGGAGA+AGG - Chr2:77864251-77864270 None:intergenic 40.0%
! TCATGCAACGTTTTTCACAG+AGG + Chr2:77864793-77864812 MsG0280010978.01.T01:CDS 40.0%
! TGCATCAAGATTGGAGTACT+TGG - Chr2:77864257-77864276 None:intergenic 40.0%
!! AGAAGAAAGGGTGTTGAGAA+TGG - Chr2:77864104-77864123 None:intergenic 40.0%
!! TATAACTGGTTTGGCAACAC+CGG - Chr2:77865727-77865746 None:intergenic 40.0%
!!! GCAGTTTTTAGAGTTCAGTC+AGG + Chr2:77866309-77866328 MsG0280010978.01.T01:three_prime_UTR 40.0%
AAAAAGCGGAGAGGGGATAA+TGG - Chr2:77864325-77864344 None:intergenic 45.0%
AATAACTCGGAGTTGAGCAG+TGG + Chr2:77864203-77864222 MsG0280010978.01.T01:CDS 45.0%
AGCAGTGGCTATAGTAGCTA+TGG + Chr2:77864218-77864237 MsG0280010978.01.T01:CDS 45.0%
AGTGGCTATAGTAGCTATGG+TGG + Chr2:77864221-77864240 MsG0280010978.01.T01:CDS 45.0%
ATAGTGTACCTGCAAATCGC+CGG - Chr2:77864397-77864416 None:intergenic 45.0%
ATGCTCCTCTGCATCAAGAT+TGG - Chr2:77864266-77864285 None:intergenic 45.0%
CAACTCCGAGTTATTCAGCT+GGG - Chr2:77864198-77864217 None:intergenic 45.0%
CTCCAAGATCTTGACAGGAA+TGG - Chr2:77865528-77865547 None:intergenic 45.0%
CTCCATTCCTGTCAAGATCT+TGG + Chr2:77865523-77865542 MsG0280010978.01.T01:intron 45.0%
CTGTTCTCCAAGATCTTGAC+AGG - Chr2:77865533-77865552 None:intergenic 45.0%
GGTACTGCAAACTTTCCTCA+CGG - Chr2:77864376-77864395 None:intergenic 45.0%
GTACTCCAATCTTGATGCAG+AGG + Chr2:77864258-77864277 MsG0280010978.01.T01:CDS 45.0%
GTGAGGAAAGTTTGCAGTAC+CGG + Chr2:77864375-77864394 MsG0280010978.01.T01:CDS 45.0%
TCAACTCCGAGTTATTCAGC+TGG - Chr2:77864199-77864218 None:intergenic 45.0%
TCCTATCCGCCAATGTCTTT+CGG - Chr2:77865951-77865970 None:intergenic 45.0%
TCTCTCCCAGCTGAATAACT+CGG + Chr2:77864190-77864209 MsG0280010978.01.T01:CDS 45.0%
TGTTTGCCGAAAGACATTGG+CGG + Chr2:77865942-77865961 MsG0280010978.01.T01:CDS 45.0%
TTCAGCAAAAAAGCGGAGAG+GGG - Chr2:77864332-77864351 None:intergenic 45.0%
!!! TTTTTAATTTTAATTTTTTG+CGG - Chr2:77864604-77864623 None:intergenic 5.0%
ATAGTCGAGTGGAGCCACAT+TGG + Chr2:77866024-77866043 MsG0280010978.01.T01:CDS 50.0%
ATGTGGCTCCACTCGACTAT+AGG - Chr2:77866024-77866043 None:intergenic 50.0%
CTTATGCATGCTACCTCCAC+AGG + Chr2:77865241-77865260 MsG0280010978.01.T01:intron 50.0%
GATAGGAGGCCACGAATTAG+GGG + Chr2:77865964-77865983 MsG0280010978.01.T01:CDS 50.0%
GCAAAACGTCCCCTAATTCG+TGG - Chr2:77865976-77865995 None:intergenic 50.0%
GGATAGGAGGCCACGAATTA+GGG + Chr2:77865963-77865982 MsG0280010978.01.T01:CDS 50.0%
! GAAAGACATTGGCGGATAGG+AGG + Chr2:77865950-77865969 MsG0280010978.01.T01:CDS 50.0%
CGGATAGGAGGCCACGAATT+AGG + Chr2:77865962-77865981 MsG0280010978.01.T01:CDS 55.0%
! AGTGGAGCCACATTGGTGGT+GGG + Chr2:77866031-77866050 MsG0280010978.01.T01:CDS 55.0%
! GCCGAAAGACATTGGCGGAT+AGG + Chr2:77865947-77865966 MsG0280010978.01.T01:CDS 55.0%
!! TCAGCTGGGAGAGAGTGTCT+TGG - Chr2:77864184-77864203 None:intergenic 55.0%
!! TTGCAGTACCGGCGATTTGC+AGG + Chr2:77864386-77864405 MsG0280010978.01.T01:CDS 55.0%
CCACAGGTAGACACTGCCAC+TGG + Chr2:77865257-77865276 MsG0280010978.01.T01:intron 60.0%
CCAGTGGCAGTGTCTACCTG+TGG - Chr2:77865260-77865279 None:intergenic 60.0%
CTTCCTCCCCACCACCAATG+TGG - Chr2:77866041-77866060 None:intergenic 60.0%
GACTCAGACGATGCTCCGTG+AGG + Chr2:77864358-77864377 MsG0280010978.01.T01:CDS 60.0%
GAGTGGAGCCACATTGGTGG+TGG + Chr2:77866030-77866049 MsG0280010978.01.T01:CDS 60.0%
GTCGAGTGGAGCCACATTGG+TGG + Chr2:77866027-77866046 MsG0280010978.01.T01:CDS 60.0%
GTGGCAGTGTCTACCTGTGG+AGG - Chr2:77865257-77865276 None:intergenic 60.0%
!! CATTGGTGGTGGGGAGGAAG+AGG + Chr2:77866041-77866060 MsG0280010978.01.T01:CDS 60.0%
!! GTGGAGCCACATTGGTGGTG+GGG + Chr2:77866032-77866051 MsG0280010978.01.T01:CDS 60.0%
!! GAGCCACATTGGTGGTGGGG+AGG + Chr2:77866035-77866054 MsG0280010978.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 77864071 77866417 77864071 ID=MsG0280010978.01;Name=MsG0280010978.01
Chr2 mRNA 77864071 77866417 77864071 ID=MsG0280010978.01.T01;Parent=MsG0280010978.01;Name=MsG0280010978.01.T01;_AED=0.48;_eAED=0.49;_QI=0|0.5|0.33|1|0.5|0.33|3|313|242
Chr2 exon 77864071 77864407 77864071 ID=MsG0280010978.01.T01:exon:11529;Parent=MsG0280010978.01.T01
Chr2 exon 77864763 77864989 77864763 ID=MsG0280010978.01.T01:exon:11530;Parent=MsG0280010978.01.T01
Chr2 exon 77865940 77866417 77865940 ID=MsG0280010978.01.T01:exon:11531;Parent=MsG0280010978.01.T01
Chr2 CDS 77864071 77864407 77864071 ID=MsG0280010978.01.T01:cds;Parent=MsG0280010978.01.T01
Chr2 CDS 77864763 77864989 77864763 ID=MsG0280010978.01.T01:cds;Parent=MsG0280010978.01.T01
Chr2 CDS 77865940 77866104 77865940 ID=MsG0280010978.01.T01:cds;Parent=MsG0280010978.01.T01
Chr2 three_prime_UTR 77866105 77866417 77866105 ID=MsG0280010978.01.T01:three_prime_utr;Parent=MsG0280010978.01.T01
Gene Sequence

>MsG0280010978.01.T01

ATGTACGCACACTCTAATTCCAACAATATTCCATTCTCAACACCCTTTCTTCTTCCATCTCCTTCTCCATTTATGGAACATATCCCTGATTTCAATGAGTTAGACTCATTCCAAGACACTCTCTCCCAGCTGAATAACTCGGAGTTGAGCAGTGGCTATAGTAGCTATGGTGGATCACCTTCTCCAAGTACTCCAATCTTGATGCAGAGGAGCATTAGCAGTCATTCCCTTCAGTACAATAATGGGACTCACCATTATCCCCTCTCCGCTTTTTTGCTGAATTGCTCGACTCAGACGATGCTCCGTGAGGAAAGTTTGCAGTACCGGCGATTTGCAGGGTTTAATAATGTTTTTTGTTTTACTAATTTCATGCAACGTTTTTCACAGAGGATTAACGGAATGCAACATAATCATCATCACGTGGATAGTCCATTGTCTAGTGAAAGTAGTATGATCATAGAAGGTATGAGCAGAGTTTGTCCATATAGCCCAGAAGAGAAGAAAGTGAGAATTGAAAGATACAGAAGAAAGAGGAATCAGAGGAACTTTAACAAGAAAATTAAGTATGTTTGCCGAAAGACATTGGCGGATAGGAGGCCACGAATTAGGGGACGTTTTGCGAGAAATGATGAAATTGATAAGAAACCTATAGTCGAGTGGAGCCACATTGGTGGTGGGGAGGAAGAGGATGAAGAAGATGAAATTGGAATAATATCTTTAATTCCATAG
Protein sequence

>MsG0280010978.01.T01

MYAHSNSNNIPFSTPFLLPSPSPFMEHIPDFNELDSFQDTLSQLNNSELSSGYSSYGGSPSPSTPILMQRSISSHSLQYNNGTHHYPLSAFLLNCSTQTMLREESLQYRRFAGFNNVFCFTNFMQRFSQRINGMQHNHHHVDSPLSSESSMIIEGMSRVCPYSPEEKKVRIERYRRKRNQRNFNKKIKYVCRKTLADRRPRIRGRFARNDEIDKKPIVEWSHIGGGEEEDEEDEIGIISLIP*