AlfalfaGEDB | MsG0280011153.01

M. sativa cultivar ZhongmuNo.1 / MsG0280011153.01

KEGG: K09422
GO: GO:0003677
PFAM: PF00249

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011153.01.T01	XP_003597573.1	91.341	358	22	5	31	381	46	401	0	600

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011153.01.T01	Q9SPG3	58.896	163	53	5	31	182	55	214	8.06E-44	158

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011153.01.T01	G7IS44	91.341	358	22	5	31	381	46	401	0.0	600

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TFs/TRs:

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Gene ID	Type	Classification
MsG0280011153.01.T01	TF	MYB-related

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011153.01.T01	MTR_2g099740	91.341	358	22	5	31	381	46	401	0.0	600
MsG0280011153.01.T01	MTR_8g017500	46.221	344	130	19	31	367	46	341	4.94e-66	213
MsG0280011153.01.T01	MTR_8g017440	60.748	214	64	12	31	236	46	247	4.14e-62	203
MsG0280011153.01.T01	MTR_8g017390	51.399	286	102	16	31	305	47	306	6.99e-62	206
MsG0280011153.01.T01	MTR_8g017540	47.840	324	121	18	31	351	92	370	1.01e-61	203
MsG0280011153.01.T01	MTR_8g017340	52.113	284	110	17	31	305	47	313	1.35e-61	206
MsG0280011153.01.T01	MTR_8g017350	52.113	284	110	17	31	305	47	313	3.45e-60	205
MsG0280011153.01.T01	MTR_2g064160	71.429	91	26	0	31	121	45	135	2.20e-39	142
MsG0280011153.01.T01	MTR_1g112760	50.000	156	65	3	31	178	47	197	6.42e-39	139
MsG0280011153.01.T01	MTR_4g105130	77.500	80	18	0	31	110	46	125	2.74e-38	141

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011153.01.T01	AT3G13890	58.896	163	53	5	31	182	46	205	6.56e-45	159
MsG0280011153.01.T01	AT3G13890	58.896	163	53	5	31	182	55	214	8.22e-45	158
MsG0280011153.01.T01	AT3G12720	77.500	80	18	0	31	110	56	135	2.68e-39	142
MsG0280011153.01.T01	AT5G12870	72.414	87	22	1	17	101	36	122	2.58e-38	139
MsG0280011153.01.T01	AT1G09540	58.197	122	39	3	6	120	25	141	6.89e-38	140
MsG0280011153.01.T01	AT5G26660	75.000	80	20	0	31	110	15	94	1.43e-37	138
MsG0280011153.01.T01	AT1G57560	63.830	94	34	0	31	124	46	139	4.38e-37	137
MsG0280011153.01.T01	AT3G08500	72.414	87	22	1	17	101	48	134	8.27e-37	137
MsG0280011153.01.T01	AT5G26660	75.000	80	20	0	31	110	46	125	8.79e-37	137
MsG0280011153.01.T01	AT1G57560	71.250	80	23	0	31	110	46	125	1.31e-36	134

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Find 65 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTCCACGTTTCAAATC+AGG	0.335974	2:+80253386	None:intergenic
AAAGTGATGATATTGATGAT+TGG	0.543382	2:+80252981	None:intergenic
AACAGATACACATTGGCCAT+TGG	0.545988	2:+80254020	None:intergenic
AAGAAGCAGAAGAAGAAAAT+GGG	0.524197	2:+80252539	None:intergenic
ACAAACATAATTATCCTTAA+TGG	0.189462	2:+80252567	None:intergenic
ACAACTTGATGATCTTGTTT+TGG	0.311200	2:+80252421	None:intergenic
ACAGTGAATATCAGAAAATG+TGG	0.592062	2:+80253065	None:intergenic
ACCAAAGAATGATCATCAAG+TGG	0.657833	2:-80252392	MsG0280011153.01.T01:CDS
ACCAGGAAGAACAGATAATG+AGG	0.592167	2:-80253157	MsG0280011153.01.T01:CDS
ACCTCATTATCTGTTCTTCC+TGG	0.311501	2:+80253156	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGAAGCAGAAGAAGAAAAT+GGG	+	Chr2:80253757-80253776	None:intergenic	30.0%
ACAGTGAATATCAGAAAATG+TGG	+	Chr2:80253231-80253250	None:intergenic	30.0%
AGAAGTGGTGAATTTAATGT+GGG	+	Chr2:80252485-80252504	None:intergenic	30.0%
ATTCAGAACATGTAGGAAAT+TGG	+	Chr2:80253438-80253457	None:intergenic	30.0%
GAAATTGGGATTATAACTGT+TGG	+	Chr2:80253423-80253442	None:intergenic	30.0%
GAAGATGGAATATTTGATGA+TGG	+	Chr2:80253485-80253504	None:intergenic	30.0%
GTATCAACCAAATTACTGAT+CGG	-	Chr2:80253810-80253829	MsG0280011153.01.T01:intron	30.0%
TACATCACTAATTATGGACA+TGG	-	Chr2:80252802-80252821	MsG0280011153.01.T01:CDS	30.0%
TATAACACCGATCAGTAATT+TGG	+	Chr2:80253820-80253839	None:intergenic	30.0%
TTCAGAACATGTAGGAAATT+GGG	+	Chr2:80253437-80253456	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	80252276	80254039	80252276	ID=MsG0280011153.01;Name=MsG0280011153.01
Chr2	mRNA	80252276	80254039	80252276	ID=MsG0280011153.01.T01;Parent=MsG0280011153.01;Name=MsG0280011153.01.T01;_AED=0.22;_eAED=0.22;_QI=0\|0\|0\|0.66\|1\|1\|3\|0\|381
Chr2	exon	80253952	80254039	80253952	ID=MsG0280011153.01.T01:exon:10275;Parent=MsG0280011153.01.T01
Chr2	exon	80253332	80253461	80253332	ID=MsG0280011153.01.T01:exon:10274;Parent=MsG0280011153.01.T01
Chr2	exon	80252276	80253203	80252276	ID=MsG0280011153.01.T01:exon:10273;Parent=MsG0280011153.01.T01
Chr2	CDS	80253952	80254039	80253952	ID=MsG0280011153.01.T01:cds;Parent=MsG0280011153.01.T01
Chr2	CDS	80253332	80253461	80253332	ID=MsG0280011153.01.T01:cds;Parent=MsG0280011153.01.T01
Chr2	CDS	80252276	80253203	80252276	ID=MsG0280011153.01.T01:cds;Parent=MsG0280011153.01.T01

Gene Sequence

>MsG0280011153.01.T01

ATGGCCAATGTGTATCTGTTAGGACATAGGACCCACAAGTCTGCATGCATGACACGTGTACAAGGTAGCCCATCTGATCAATCTGAGAGACTGCAGAGATGTGGAAAAAGTTGCAGATTGAGATGGATAAATTATTTAAGACCTGATTTGAAACGTGGAAGTTTTTCTCCACAAGAAGCTGCTCTCATTATTGAACTTCATACTATTCTAGGCAACAGGTGGGCTCAGATAGCAAAGCATTTACCAGGAAGAACAGATAATGAGGTGAAGAACTTTTGGAATTCAAGCATAAAGAAGAAACTACTTTCACATGATTTTGTTCCCACTTCTTTAGCCACATTTTCTGATATTCACTGTCCTAGAAATGCTTCTGTTGAAAGTTTCTTCCCTTTATTTAATGACAATCCTATCCTTAACTCCAATCATCAATATCATCACTTTGACAACCACCTATACCTTCCCATCAATATCCCAACTCCAATCCTACAAGGTATTGATCATCAAAATGATATCAAAAATATTGATACCAACAGTTATAATCCCAATTTCCTACATGTTCTGAATAATCCAATATTGCCTGAAACAGTACCATCATCAAATATTCCATCTTCTTATGAATTAGATACATGGTCATTAATTCATCATCTCAATCCAAATATTCAAGAAAATCATCAAATCACCACCAAAAGTGATGTTGCAACCCAAAATTATAATATTGTTGATCAGAATTTTATCAATATGCCAAATACATGGCAGCAGCAGCACTATGATAGTAATAGTCATTTAGTGAATCACCTTGACCCTATTGTGCAAAAAGTCTTTGAAAGTGAAACCATTAAGGATAATTATGTTTGTAGCGTCCCATTTTCTTCTTCTGCTTCTTCACAAGAACATGATCATCATCATCATCATGATGGTATCAACCAAATTACTGATCGGTGTTATAATACAGATCATTATCAAGGTGCTATAATTTGTCCAAAACAAGATCATCAAGTTGTTGCTGCACCAAAGAATGATCATCAAGTGGAGTATAACATCGAAGCTCTCATCATGTCATCAATGCCATCTTCAACAACAACATCTTCTTCATCACCACTATCATTCTATCAACCAGTCACTCTCACAAAACCTATCCTTCCTTAA

Protein sequence

>MsG0280011153.01.T01

MANVYLLGHRTHKSACMTRVQGSPSDQSERLQRCGKSCRLRWINYLRPDLKRGSFSPQEAALIIELHTILGNRWAQIAKHLPGRTDNEVKNFWNSSIKKKLLSHDFVPTSLATFSDIHCPRNASVESFFPLFNDNPILNSNHQYHHFDNHLYLPINIPTPILQGIDHQNDIKNIDTNSYNPNFLHVLNNPILPETVPSSNIPSSYELDTWSLIHHLNPNIQENHQITTKSDVATQNYNIVDQNFINMPNTWQQQHYDSNSHLVNHLDPIVQKVFESETIKDNYVCSVPFSSSASSQEHDHHHHHDGINQITDRCYNTDHYQGAIICPKQDHQVVAAPKNDHQVEYNIEALIMSSMPSSTTTSSSSPLSFYQPVTLTKPILP*