Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011479.01.T01 | QSD99670.1 | 82.353 | 51 | 9 | 0 | 55 | 105 | 164 | 214 | 1.46E-17 | 85.9 |
| MsG0380011479.01.T01 | QSD99670.1 | 77.273 | 44 | 10 | 0 | 1 | 44 | 1 | 44 | 8.22E-14 | 75.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011479.01.T01 | A0A896WC68 | 82.353 | 51 | 9 | 0 | 55 | 105 | 164 | 214 | 6.98e-18 | 85.9 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0380011479.01.T01 | TF | MYB-related |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011479.01.T01 | MTR_1g110460 | 64.286 | 42 | 15 | 0 | 3 | 44 | 19 | 60 | 3.06e-12 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGACTGAGGCATAATATT+TGG | 0.224253 | 3:-4149 | None:intergenic |
| AAATATTATGCCTCAGTCTT+TGG | 0.239938 | 3:+4151 | MsG0380011479.01.T01:CDS |
| ATCTCGTAAAGATTCTTATA+AGG | 0.342702 | 3:-4065 | None:intergenic |
| AGTTTCTTGGCTATGAGTTC+AGG | 0.360361 | 3:+3736 | MsG0380011479.01.T01:CDS |
| AAGATGACTATGAACTTAGA+AGG | 0.363354 | 3:+3644 | MsG0380011479.01.T01:CDS |
| TGAAGGGCGATGGAGTTTCT+TGG | 0.418507 | 3:+3723 | MsG0380011479.01.T01:CDS |
| GAGAAGCTTGCCATGCATAA+TGG | 0.434273 | 3:+3619 | None:intergenic |
| ATCAAACACATTGCTAATCA+TGG | 0.478221 | 3:+3700 | MsG0380011479.01.T01:CDS |
| GTCTTTAGATAACAATGAGT+TGG | 0.516633 | 3:+4196 | MsG0380011479.01.T01:CDS |
| CTAATCATGGTGAAGGGCGA+TGG | 0.518933 | 3:+3713 | MsG0380011479.01.T01:CDS |
| CACATTGCTAATCATGGTGA+AGG | 0.532252 | 3:+3706 | MsG0380011479.01.T01:CDS |
| TCTTCCTCTTCAAGAGTCCA+AGG | 0.541368 | 3:-3670 | None:intergenic |
| GTCATCTTTACCATTATGCA+TGG | 0.541953 | 3:-3629 | None:intergenic |
| CAGTCTTTGGAATACACTAC+TGG | 0.547619 | 3:+4164 | MsG0380011479.01.T01:CDS |
| ATGAACTTAGAAGGGAACCT+TGG | 0.552788 | 3:+3653 | MsG0380011479.01.T01:CDS |
| AGATGACTATGAACTTAGAA+GGG | 0.614691 | 3:+3645 | MsG0380011479.01.T01:CDS |
| GGAACCTTGGACTCTTGAAG+AGG | 0.641233 | 3:+3666 | MsG0380011479.01.T01:CDS |
| ACATTGCTAATCATGGTGAA+GGG | 0.649010 | 3:+3707 | MsG0380011479.01.T01:CDS |
| GATTAGCAATGTGTTTGATG+AGG | 0.678454 | 3:-3696 | None:intergenic |
| GTAGTGTATTCCAAAGACTG+AGG | 0.730832 | 3:-4161 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATCTTTTTGATTTAGGATTA+AGG | + | Chr3:3833-3852 | MsG0380011479.01.T01:intron | 20.0% |
| !!! | GATTGTTATCTTTTTGATTT+AGG | + | Chr3:3826-3845 | MsG0380011479.01.T01:intron | 20.0% |
| ! | ATCTCGTAAAGATTCTTATA+AGG | - | Chr3:4068-4087 | None:intergenic | 25.0% |
| !! | AATCAAAAACTTTTCTTCTG+AGG | - | Chr3:3927-3946 | None:intergenic | 25.0% |
| !! | ATTCTTATAAGGACTTTTGT+TGG | - | Chr3:4057-4076 | None:intergenic | 25.0% |
| !! | TTAAGTTTTCTCTTTATGTC+AGG | - | Chr3:3904-3923 | None:intergenic | 25.0% |
| !!! | GATATGGTATTTTTTTCTGT+AGG | + | Chr3:3780-3799 | MsG0380011479.01.T01:intron | 25.0% |
| !!! | TTTTTGATTTAGGATTAAGG+AGG | + | Chr3:3836-3855 | MsG0380011479.01.T01:intron | 25.0% |
| AAAGACTGAGGCATAATATT+TGG | - | Chr3:4152-4171 | None:intergenic | 30.0% | |
| AAATATTATGCCTCAGTCTT+TGG | + | Chr3:4151-4170 | MsG0380011479.01.T01:CDS | 30.0% | |
| AAGATGACTATGAACTTAGA+AGG | + | Chr3:3644-3663 | MsG0380011479.01.T01:CDS | 30.0% | |
| AGATGACTATGAACTTAGAA+GGG | + | Chr3:3645-3664 | MsG0380011479.01.T01:CDS | 30.0% | |
| ATCAAACACATTGCTAATCA+TGG | + | Chr3:3700-3719 | MsG0380011479.01.T01:CDS | 30.0% | |
| GATGAATAATGCTATTGTAG+AGG | - | Chr3:4021-4040 | None:intergenic | 30.0% | |
| GTCTTTAGATAACAATGAGT+TGG | + | Chr3:4196-4215 | MsG0380011479.01.T01:CDS | 30.0% | |
| ACATTGCTAATCATGGTGAA+GGG | + | Chr3:3707-3726 | MsG0380011479.01.T01:CDS | 35.0% | |
| GATTAGCAATGTGTTTGATG+AGG | - | Chr3:3699-3718 | None:intergenic | 35.0% | |
| ! | TTTTGAACTCCACTCAAAGT+GGG | + | Chr3:3946-3965 | MsG0380011479.01.T01:intron | 35.0% |
| ! | TTTTTGAACTCCACTCAAAG+TGG | + | Chr3:3945-3964 | MsG0380011479.01.T01:intron | 35.0% |
| !! | AAGTTTGCTAGTGCAAGATA+TGG | + | Chr3:3764-3783 | MsG0380011479.01.T01:intron | 35.0% |
| AGTTTCTTGGCTATGAGTTC+AGG | + | Chr3:3736-3755 | MsG0380011479.01.T01:CDS | 40.0% | |
| ATGAACTTAGAAGGGAACCT+TGG | + | Chr3:3653-3672 | MsG0380011479.01.T01:CDS | 40.0% | |
| CACATTGCTAATCATGGTGA+AGG | + | Chr3:3706-3725 | MsG0380011479.01.T01:CDS | 40.0% | |
| GTAGTGTATTCCAAAGACTG+AGG | - | Chr3:4164-4183 | None:intergenic | 40.0% | |
| TTTGAACTCCACTCAAAGTG+GGG | + | Chr3:3947-3966 | MsG0380011479.01.T01:intron | 40.0% | |
| ! | GATTTAGGATTAAGGAGGAC+AGG | + | Chr3:3841-3860 | MsG0380011479.01.T01:intron | 40.0% |
| !! | CAGTCTTTGGAATACACTAC+TGG | + | Chr3:4164-4183 | MsG0380011479.01.T01:CDS | 40.0% |
| TCTTCCTCTTCAAGAGTCCA+AGG | - | Chr3:3673-3692 | None:intergenic | 45.0% | |
| CTAATCATGGTGAAGGGCGA+TGG | + | Chr3:3713-3732 | MsG0380011479.01.T01:CDS | 50.0% | |
| GGAACCTTGGACTCTTGAAG+AGG | + | Chr3:3666-3685 | MsG0380011479.01.T01:CDS | 50.0% | |
| TGAAGGGCGATGGAGTTTCT+TGG | + | Chr3:3723-3742 | MsG0380011479.01.T01:CDS | 50.0% | |
| ! | ACTTGTTGCCCCACTTTGAG+TGG | - | Chr3:3958-3977 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 3631 | 4223 | 3631 | ID=MsG0380011479.01;Name=MsG0380011479.01 |
| Chr3 | mRNA | 3631 | 4223 | 3631 | ID=MsG0380011479.01.T01;Parent=MsG0380011479.01;Name=MsG0380011479.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|0|0|2|0|106 |
| Chr3 | exon | 3631 | 3757 | 3631 | ID=MsG0380011479.01.T01:exon:280;Parent=MsG0380011479.01.T01 |
| Chr3 | exon | 4030 | 4223 | 4030 | ID=MsG0380011479.01.T01:exon:279;Parent=MsG0380011479.01.T01 |
| Chr3 | CDS | 3631 | 3757 | 3631 | ID=MsG0380011479.01.T01:cds;Parent=MsG0380011479.01.T01 |
| Chr3 | CDS | 4030 | 4223 | 4030 | ID=MsG0380011479.01.T01:cds;Parent=MsG0380011479.01.T01 |
| Gene Sequence |
| Protein sequence |
