AlfalfaGEDB | MsG0380011479.01

M. sativa cultivar ZhongmuNo.1 / MsG0380011479.01

KEGG: K09422
GO: GO:0003677
PFAM: PF00249

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380011479.01.T01	QSD99670.1	82.353	51	9	0	55	105	164	214	1.46E-17	85.9
MsG0380011479.01.T01	QSD99670.1	77.273	44	10	0	1	44	1	44	8.22E-14	75.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380011479.01.T01	A0A896WC68	82.353	51	9	0	55	105	164	214	6.98e-18	85.9

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TFs/TRs:

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Gene ID	Type	Classification
MsG0380011479.01.T01	TF	MYB-related

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380011479.01.T01	MTR_1g110460	64.286	42	15	0	3	44	19	60	3.06e-12	61.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 20 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGACTGAGGCATAATATT+TGG	0.224253	3:-4149	None:intergenic
AAATATTATGCCTCAGTCTT+TGG	0.239938	3:+4151	MsG0380011479.01.T01:CDS
AAGATGACTATGAACTTAGA+AGG	0.363354	3:+3644	MsG0380011479.01.T01:CDS
ACATTGCTAATCATGGTGAA+GGG	0.649010	3:+3707	MsG0380011479.01.T01:CDS
AGATGACTATGAACTTAGAA+GGG	0.614691	3:+3645	MsG0380011479.01.T01:CDS
AGTTTCTTGGCTATGAGTTC+AGG	0.360361	3:+3736	MsG0380011479.01.T01:CDS
ATCAAACACATTGCTAATCA+TGG	0.478221	3:+3700	MsG0380011479.01.T01:CDS
ATCTCGTAAAGATTCTTATA+AGG	0.342702	3:-4065	None:intergenic
ATGAACTTAGAAGGGAACCT+TGG	0.552788	3:+3653	MsG0380011479.01.T01:CDS
CACATTGCTAATCATGGTGA+AGG	0.532252	3:+3706	MsG0380011479.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGACTGAGGCATAATATT+TGG	-	Chr3:4152-4171	None:intergenic	30.0%
AAATATTATGCCTCAGTCTT+TGG	+	Chr3:4151-4170	MsG0380011479.01.T01:CDS	30.0%
AAGATGACTATGAACTTAGA+AGG	+	Chr3:3644-3663	MsG0380011479.01.T01:CDS	30.0%
AGATGACTATGAACTTAGAA+GGG	+	Chr3:3645-3664	MsG0380011479.01.T01:CDS	30.0%
ATCAAACACATTGCTAATCA+TGG	+	Chr3:3700-3719	MsG0380011479.01.T01:CDS	30.0%
GATGAATAATGCTATTGTAG+AGG	-	Chr3:4021-4040	None:intergenic	30.0%
GTCTTTAGATAACAATGAGT+TGG	+	Chr3:4196-4215	MsG0380011479.01.T01:CDS	30.0%
ACATTGCTAATCATGGTGAA+GGG	+	Chr3:3707-3726	MsG0380011479.01.T01:CDS	35.0%
GATTAGCAATGTGTTTGATG+AGG	-	Chr3:3699-3718	None:intergenic	35.0%
AGTTTCTTGGCTATGAGTTC+AGG	+	Chr3:3736-3755	MsG0380011479.01.T01:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	3631	4223	3631	ID=MsG0380011479.01;Name=MsG0380011479.01
Chr3	mRNA	3631	4223	3631	ID=MsG0380011479.01.T01;Parent=MsG0380011479.01;Name=MsG0380011479.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|0\|0\|1\|0\|0\|2\|0\|106
Chr3	exon	3631	3757	3631	ID=MsG0380011479.01.T01:exon:280;Parent=MsG0380011479.01.T01
Chr3	exon	4030	4223	4030	ID=MsG0380011479.01.T01:exon:279;Parent=MsG0380011479.01.T01
Chr3	CDS	3631	3757	3631	ID=MsG0380011479.01.T01:cds;Parent=MsG0380011479.01.T01
Chr3	CDS	4030	4223	4030	ID=MsG0380011479.01.T01:cds;Parent=MsG0380011479.01.T01

Gene Sequence	>MsG0380011479.01.T01 ATGCATAATGGTAAAGATGACTATGAACTTAGAAGGGAACCTTGGACTCTTGAAGAGGAAGATCTCCTCATCAAACACATTGCTAATCATGGTGAAGGGCGATGGAGTTTCTTGGCTATGAGTTCAGCATTATTCATCAATTGTGACTACACCAACAAAAGTCCTTATAAGAATCTTTACGAGATAAACTCAGATTCTGAGCACAACAATTGTTTATGCATGTCTTCTTTAGAATCAACAAATATGCCAAATATTATGCCTCAGTCTTTGGAATACACTACTGGTCAATTTCAGTCTTTAGATAACAATGAGTTGGCATAA
Protein sequence	>MsG0380011479.01.T01 MHNGKDDYELRREPWTLEEEDLLIKHIANHGEGRWSFLAMSSALFINCDYTNKSPYKNLYEINSDSEHNNCLCMSSLESTNMPNIMPQSLEYTTGQFQSLDNNELA*