Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011481.01.T01 | QSD99665.1 | 64.815 | 54 | 19 | 0 | 4 | 57 | 50 | 103 | 1.48E-15 | 80.9 |
| MsG0380011481.01.T01 | QSD99665.1 | 86.667 | 45 | 5 | 1 | 47 | 90 | 213 | 257 | 1.73E-13 | 75.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011481.01.T01 | A0A896WCU1 | 64.815 | 54 | 19 | 0 | 4 | 57 | 50 | 103 | 7.09e-16 | 80.9 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0380011481.01.T01 | TF | MYB-related |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380011481.01.T01 | MTR_1g110460 | 63.415 | 41 | 15 | 0 | 3 | 43 | 19 | 59 | 9.94e-12 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGACTGAGGCATAATATT+TGG | 0.224253 | 3:+51108 | None:intergenic |
| AAATATTATGCCTCAGTCTT+TGG | 0.239938 | 3:-51106 | MsG0380011481.01.T01:CDS |
| TCTTTAGATAACAATGAGTT+TGG | 0.334321 | 3:-51060 | MsG0380011481.01.T01:CDS |
| AGTTTCTTGGCTATGAGTTC+AGG | 0.360361 | 3:-51520 | MsG0380011481.01.T01:intron |
| AAGATGACTATGAACTTAGA+AGG | 0.363354 | 3:-51612 | MsG0380011481.01.T01:CDS |
| TGAAGGGCGATGGAGTTTCT+TGG | 0.418507 | 3:-51533 | MsG0380011481.01.T01:CDS |
| GAGAAGCTTGCCATGCATAA+TGG | 0.434273 | 3:-51637 | None:intergenic |
| ATCAAACACATTGCTAATCA+TGG | 0.478221 | 3:-51556 | MsG0380011481.01.T01:CDS |
| CTAATCATGGTGAAGGGCGA+TGG | 0.518933 | 3:-51543 | MsG0380011481.01.T01:CDS |
| CACATTGCTAATCATGGTGA+AGG | 0.532252 | 3:-51550 | MsG0380011481.01.T01:CDS |
| TCTTCCTCTTCAAGAGTCCA+AGG | 0.541368 | 3:+51586 | None:intergenic |
| GTCATCTTTACCATTATGCA+TGG | 0.541953 | 3:+51627 | None:intergenic |
| TTCAAGGTCAAGGAACTATG+AGG | 0.543361 | 3:+51016 | None:intergenic |
| CAGTCTTTGGAATACACTAC+TGG | 0.547619 | 3:-51093 | MsG0380011481.01.T01:CDS |
| ATGAACTTAGAAGGGAACCT+TGG | 0.552788 | 3:-51603 | MsG0380011481.01.T01:CDS |
| TTTGGCATAAGTGCTTATGA+TGG | 0.595491 | 3:-51042 | MsG0380011481.01.T01:CDS |
| AGATGACTATGAACTTAGAA+GGG | 0.614691 | 3:-51611 | MsG0380011481.01.T01:CDS |
| GGAACCTTGGACTCTTGAAG+AGG | 0.641233 | 3:-51590 | MsG0380011481.01.T01:CDS |
| ACATTGCTAATCATGGTGAA+GGG | 0.649010 | 3:-51549 | MsG0380011481.01.T01:CDS |
| GATTAGCAATGTGTTTGATG+AGG | 0.678454 | 3:+51560 | None:intergenic |
| GTAGTGTATTCCAAAGACTG+AGG | 0.730832 | 3:+51096 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCTTTTTTGATTTAGGATTA+AGG | - | Chr3:51223-51242 | MsG0380011481.01.T01:intron | 20.0% |
| !!! | TGATTGTTCTTTTTTGATTT+AGG | - | Chr3:51216-51235 | MsG0380011481.01.T01:intron | 20.0% |
| ! | ATCTCGTAAAGATTCTTATA+AGG | + | Chr3:51460-51479 | None:intergenic | 25.0% |
| ! | TCTCGTAAAGATTCTTATAA+GGG | + | Chr3:51459-51478 | None:intergenic | 25.0% |
| ! | TCTTTAGATAACAATGAGTT+TGG | - | Chr3:51588-51607 | MsG0380011481.01.T01:CDS | 25.0% |
| !! | AATCAAAAACTTTTCTTCTG+AGG | + | Chr3:51318-51337 | None:intergenic | 25.0% |
| !! | TAAGTTTTCTCTTTTATGTC+AGG | + | Chr3:51294-51313 | None:intergenic | 25.0% |
| !!! | AGATATGGTATTTTTTCTGT+AGG | - | Chr3:51171-51190 | MsG0380011481.01.T01:intron | 25.0% |
| !!! | TTTTTGATTTAGGATTAAGG+AGG | - | Chr3:51226-51245 | MsG0380011481.01.T01:intron | 25.0% |
| AAAGACTGAGGCATAATATT+TGG | + | Chr3:51543-51562 | None:intergenic | 30.0% | |
| AAATATTATGCCTCAGTCTT+TGG | - | Chr3:51542-51561 | MsG0380011481.01.T01:CDS | 30.0% | |
| AAGATGACTATGAACTTAGA+AGG | - | Chr3:51036-51055 | MsG0380011481.01.T01:CDS | 30.0% | |
| AGATGACTATGAACTTAGAA+GGG | - | Chr3:51037-51056 | MsG0380011481.01.T01:CDS | 30.0% | |
| ATCAAACACATTGCTAATCA+TGG | - | Chr3:51092-51111 | MsG0380011481.01.T01:CDS | 30.0% | |
| GATGAATAATGCTATTGTAG+AGG | + | Chr3:51412-51431 | None:intergenic | 30.0% | |
| ! | TTCTTATAAGGGACTTTTGT+TGG | + | Chr3:51448-51467 | None:intergenic | 30.0% |
| ACATTGCTAATCATGGTGAA+GGG | - | Chr3:51099-51118 | MsG0380011481.01.T01:CDS | 35.0% | |
| GATTAGCAATGTGTTTGATG+AGG | + | Chr3:51091-51110 | None:intergenic | 35.0% | |
| TTTGGCATAAGTGCTTATGA+TGG | - | Chr3:51606-51625 | MsG0380011481.01.T01:CDS | 35.0% | |
| ! | TTTTGAACTCCACTCAAAGT+GGG | - | Chr3:51337-51356 | MsG0380011481.01.T01:intron | 35.0% |
| ! | TTTTTGAACTCCACTCAAAG+TGG | - | Chr3:51336-51355 | MsG0380011481.01.T01:intron | 35.0% |
| !! | AAGTTTGCTAGTGCAAGATA+TGG | - | Chr3:51156-51175 | MsG0380011481.01.T01:intron | 35.0% |
| AGTTTCTTGGCTATGAGTTC+AGG | - | Chr3:51128-51147 | MsG0380011481.01.T01:CDS | 40.0% | |
| ATGAACTTAGAAGGGAACCT+TGG | - | Chr3:51045-51064 | MsG0380011481.01.T01:CDS | 40.0% | |
| CACATTGCTAATCATGGTGA+AGG | - | Chr3:51098-51117 | MsG0380011481.01.T01:CDS | 40.0% | |
| GTAGTGTATTCCAAAGACTG+AGG | + | Chr3:51555-51574 | None:intergenic | 40.0% | |
| TTTGAACTCCACTCAAAGTG+GGG | - | Chr3:51338-51357 | MsG0380011481.01.T01:intron | 40.0% | |
| ! | GATTTAGGATTAAGGAGGAC+AGG | - | Chr3:51231-51250 | MsG0380011481.01.T01:intron | 40.0% |
| !! | CAGTCTTTGGAATACACTAC+TGG | - | Chr3:51555-51574 | MsG0380011481.01.T01:CDS | 40.0% |
| TCTTCCTCTTCAAGAGTCCA+AGG | + | Chr3:51065-51084 | None:intergenic | 45.0% | |
| CTAATCATGGTGAAGGGCGA+TGG | - | Chr3:51105-51124 | MsG0380011481.01.T01:CDS | 50.0% | |
| GGAACCTTGGACTCTTGAAG+AGG | - | Chr3:51058-51077 | MsG0380011481.01.T01:CDS | 50.0% | |
| TGAAGGGCGATGGAGTTTCT+TGG | - | Chr3:51115-51134 | MsG0380011481.01.T01:CDS | 50.0% | |
| ! | ACTTGTTGCCCCACTTTGAG+TGG | + | Chr3:51349-51368 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 51023 | 51647 | 51023 | ID=MsG0380011481.01;Name=MsG0380011481.01 |
| Chr3 | mRNA | 51023 | 51647 | 51023 | ID=MsG0380011481.01.T01;Parent=MsG0380011481.01;Name=MsG0380011481.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|0|0|2|0|92 |
| Chr3 | exon | 51521 | 51647 | 51521 | ID=MsG0380011481.01.T01:exon:281;Parent=MsG0380011481.01.T01 |
| Chr3 | exon | 51023 | 51174 | 51023 | ID=MsG0380011481.01.T01:exon:282;Parent=MsG0380011481.01.T01 |
| Chr3 | CDS | 51521 | 51647 | 51521 | ID=MsG0380011481.01.T01:cds;Parent=MsG0380011481.01.T01 |
| Chr3 | CDS | 51023 | 51174 | 51023 | ID=MsG0380011481.01.T01:cds;Parent=MsG0380011481.01.T01 |
| Gene Sequence |
| Protein sequence |
