AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013037.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013037.01.T02 MTR_5g020580 83.871 155 23 1 1 155 89 241 1.06e-87 262
MsG0380013037.01.T02 MTR_8g077195 62.581 155 56 1 1 155 87 239 5.33e-65 204
MsG0380013037.01.T02 MTR_8g077620 56.410 156 61 3 1 155 86 235 2.52e-52 171
MsG0380013037.01.T02 MTR_8g077675 53.846 156 66 3 1 155 82 232 2.58e-51 168
MsG0380013037.01.T02 MTR_1g022370 50.633 158 73 3 1 155 104 259 1.52e-45 154
MsG0380013037.01.T02 MTR_2g020280 51.613 155 73 1 1 155 73 225 4.17e-43 147
MsG0380013037.01.T02 MTR_1g098790 50.318 157 73 4 1 155 87 240 3.64e-41 142
MsG0380013037.01.T02 MTR_2g007930 50.943 159 71 5 1 155 88 243 1.12e-40 141
MsG0380013037.01.T02 MTR_2g076200 45.161 155 83 1 1 155 76 228 1.17e-38 135
MsG0380013037.01.T02 MTR_0210s0010 68.750 96 24 1 10 105 9 98 2.06e-36 122
MsG0380013037.01.T02 MTR_1g088960 48.000 150 75 2 1 150 84 230 1.33e-35 127
MsG0380013037.01.T02 MTR_2g076210 42.308 156 83 2 1 155 71 220 1.28e-33 121
MsG0380013037.01.T02 MTR_8g077640 51.908 131 40 3 3 115 131 256 4.19e-33 120
MsG0380013037.01.T02 MTR_2g020280 51.613 124 58 1 32 155 5 126 2.92e-31 114
MsG0380013037.01.T02 MTR_8g077650 46.970 132 59 6 16 143 44 168 2.19e-27 104
MsG0380013037.01.T02 MTR_0479s0040 66.667 72 18 1 24 95 10 75 9.15e-24 89.0
MsG0380013037.01.T02 MTR_1g023800 47.945 73 38 0 1 73 86 158 3.78e-16 71.6
MsG0380013037.01.T01 MTR_5g020580 83.019 159 25 1 1 159 85 241 5.39e-89 266
MsG0380013037.01.T01 MTR_8g077195 62.025 158 58 1 2 159 84 239 1.02e-65 206
MsG0380013037.01.T01 MTR_8g077620 55.975 159 63 3 2 159 83 235 4.54e-53 173
MsG0380013037.01.T01 MTR_8g077675 53.459 159 68 3 2 159 79 232 6.85e-52 170
MsG0380013037.01.T01 MTR_1g022370 50.000 162 76 3 1 159 100 259 1.64e-46 157
MsG0380013037.01.T01 MTR_2g020280 50.943 159 76 1 1 159 69 225 4.67e-44 149
MsG0380013037.01.T01 MTR_1g098790 49.689 161 76 4 1 159 83 240 3.27e-42 145
MsG0380013037.01.T01 MTR_2g007930 50.307 163 74 5 1 159 84 243 1.89e-41 143
MsG0380013037.01.T01 MTR_2g076200 45.625 160 83 3 1 159 72 228 2.91e-39 136
MsG0380013037.01.T01 MTR_1g088960 47.403 154 78 2 1 154 80 230 1.16e-36 130
MsG0380013037.01.T01 MTR_0210s0010 68.750 96 24 1 14 109 9 98 2.58e-36 121
MsG0380013037.01.T01 MTR_2g076210 41.875 160 86 2 1 159 67 220 2.02e-34 124
MsG0380013037.01.T01 MTR_8g077640 51.908 131 40 3 7 119 131 256 3.83e-33 120
MsG0380013037.01.T01 MTR_2g020280 51.613 124 58 1 36 159 5 126 3.56e-31 114
MsG0380013037.01.T01 MTR_8g077650 46.970 132 59 6 20 147 44 168 2.99e-27 104
MsG0380013037.01.T01 MTR_0479s0040 66.667 72 18 1 28 99 10 75 1.09e-23 88.6
MsG0380013037.01.T01 MTR_1g023800 46.753 77 41 0 1 77 82 158 4.22e-17 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013037.01.T02 AT2G18280 54.839 155 65 2 1 155 82 231 3.96e-52 170
MsG0380013037.01.T02 AT2G18280 54.839 155 65 2 1 155 82 231 3.96e-52 170
MsG0380013037.01.T02 AT1G47270 49.315 146 63 3 1 145 78 213 5.97e-41 141
MsG0380013037.01.T02 AT1G47270 49.315 146 63 3 1 145 102 237 8.96e-41 141
MsG0380013037.01.T02 AT1G47270 49.315 146 63 3 1 145 103 238 9.51e-41 141
MsG0380013037.01.T02 AT1G76900 50.000 160 72 4 1 155 91 247 1.19e-40 142
MsG0380013037.01.T02 AT1G76900 50.000 160 72 4 1 155 91 247 1.19e-40 142
MsG0380013037.01.T02 AT1G76900 50.000 160 72 4 1 155 91 247 1.19e-40 142
MsG0380013037.01.T02 AT1G25280 52.229 157 73 2 1 155 93 249 2.64e-39 138
MsG0380013037.01.T02 AT1G53320 44.516 155 84 1 1 155 78 230 6.23e-39 135
MsG0380013037.01.T02 AT2G47900 48.408 157 77 2 1 155 86 240 7.14e-39 136
MsG0380013037.01.T02 AT2G47900 48.718 156 77 2 1 155 86 239 1.06e-38 135
MsG0380013037.01.T02 AT2G47900 48.718 156 77 2 1 155 86 239 1.06e-38 135
MsG0380013037.01.T02 AT3G06380 47.742 155 75 3 1 155 68 216 1.10e-37 132
MsG0380013037.01.T02 AT1G43640 44.304 158 82 4 1 155 23 177 2.55e-36 129
MsG0380013037.01.T02 AT1G43640 44.304 158 82 4 1 155 89 243 6.56e-36 129
MsG0380013037.01.T02 AT5G18680 46.452 155 80 1 1 155 74 225 6.54e-35 125
MsG0380013037.01.T02 AT5G18680 45.385 130 68 1 26 155 7 133 1.24e-25 99.4
MsG0380013037.01.T02 AT3G06380 45.238 126 63 3 30 155 5 124 1.92e-25 98.6
MsG0380013037.01.T02 AT1G25280 50.704 71 34 1 86 155 1 71 1.73e-12 63.5
MsG0380013037.01.T02 AT1G25280 50.704 71 34 1 86 155 1 71 1.73e-12 63.5
MsG0380013037.01.T01 AT2G18280 54.717 159 67 2 1 159 78 231 2.04e-53 174
MsG0380013037.01.T01 AT2G18280 54.717 159 67 2 1 159 78 231 2.04e-53 174
MsG0380013037.01.T01 AT1G47270 48.667 150 66 3 1 149 74 213 5.49e-42 144
MsG0380013037.01.T01 AT1G47270 48.667 150 66 3 1 149 98 237 7.41e-42 144
MsG0380013037.01.T01 AT1G47270 48.667 150 66 3 1 149 99 238 7.87e-42 144
MsG0380013037.01.T01 AT1G76900 49.390 164 75 4 1 159 87 247 1.07e-41 145
MsG0380013037.01.T01 AT1G76900 49.390 164 75 4 1 159 87 247 1.07e-41 145
MsG0380013037.01.T01 AT1G76900 49.390 164 75 4 1 159 87 247 1.07e-41 145
MsG0380013037.01.T01 AT1G25280 51.553 161 76 2 1 159 89 249 2.70e-40 140
MsG0380013037.01.T01 AT2G47900 48.447 161 79 2 1 159 82 240 3.15e-40 140
MsG0380013037.01.T01 AT2G47900 48.750 160 79 2 1 159 82 239 4.62e-40 139
MsG0380013037.01.T01 AT2G47900 48.750 160 79 2 1 159 82 239 4.62e-40 139
MsG0380013037.01.T01 AT1G53320 44.025 159 87 1 1 159 74 230 1.09e-39 138
MsG0380013037.01.T01 AT3G06380 47.170 159 78 3 1 159 64 216 9.43e-39 135
MsG0380013037.01.T01 AT1G43640 43.827 162 85 4 1 159 19 177 3.10e-37 131
MsG0380013037.01.T01 AT1G43640 43.827 162 85 4 1 159 85 243 8.39e-37 131
MsG0380013037.01.T01 AT5G18680 45.912 159 83 1 1 159 70 225 6.40e-36 128
MsG0380013037.01.T01 AT5G18680 45.385 130 68 1 30 159 7 133 1.47e-25 99.4
MsG0380013037.01.T01 AT3G06380 45.238 126 63 3 34 159 5 124 2.21e-25 98.6
MsG0380013037.01.T01 AT1G25280 50.704 71 34 1 90 159 1 71 2.21e-12 63.5
MsG0380013037.01.T01 AT1G25280 50.704 71 34 1 90 159 1 71 2.21e-12 63.5
MsG0380013037.01.T01 AT1G61940 43.333 60 34 0 1 60 28 87 7.23e-12 62.0

Find 41 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAACTCAGGTCTAACATTT+TGG 0.098131 3:-29107539 MsG0380013037.01.T01:CDS
CTCCTACTGGACCGGCTATT+TGG 0.181607 3:+29107457 None:intergenic
CCAGTAGGAGATTTCTTTCT+AGG 0.241845 3:-29107444 MsG0380013037.01.T01:CDS
CGTCAACCTTCCACACTCTT+CGG 0.358084 3:+29107792 None:intergenic
CACAGACTTAATCATATCTA+TGG 0.360821 3:-29107602 MsG0380013037.01.T01:CDS
GTCAACCTTCCACACTCTTC+GGG 0.361120 3:+29107793 None:intergenic
TCATATTTCGTTAAATCAGC+TGG 0.364782 3:-29107767 MsG0380013037.01.T01:CDS
TCTGCGTATCTATTGTGTTA+TGG 0.366501 3:-29107694 MsG0380013037.01.T01:CDS
TAGTTATATGCTTGAACTCT+TGG 0.377661 3:+29107412 None:intergenic
AGGCAACCTCCACACGGATC+TGG 0.416160 3:-29107487 MsG0380013037.01.T01:CDS
CTCCGATACAATGCTTCATC+AGG 0.436765 3:-29107732 MsG0380013037.01.T01:CDS
ATAAGAGCAATTCAGCTCAT+AGG 0.461752 3:+29107373 None:intergenic
TAGGAGATTTCTTTCTAGGC+AGG 0.469861 3:-29107440 MsG0380013037.01.T01:CDS
CGTTCCAGCAATGCGTATGT+TGG 0.488866 3:-29107562 MsG0380013037.01.T01:CDS
CATATTTCGTTAAATCAGCT+GGG 0.507372 3:-29107766 MsG0380013037.01.T01:CDS
GTACTCCTGTGTGTGAATCA+TGG 0.512410 3:-29107846 MsG0380013037.01.T01:CDS
CCTAGAAAGAAATCTCCTAC+TGG 0.516235 3:+29107444 None:intergenic
TCACTATATACGACATTGAC+AGG 0.521151 3:-29107507 MsG0380013037.01.T01:CDS
AAAGAAATCTCCTACTGGAC+CGG 0.552476 3:+29107449 None:intergenic
GTATCTATTGTGTTATGGAC+TGG 0.561620 3:-29107689 MsG0380013037.01.T01:CDS
AGATATGATTAAGTCTGTGC+CGG 0.566788 3:+29107606 None:intergenic
CGATACAATGCTTCATCAGG+AGG 0.568935 3:-29107729 MsG0380013037.01.T01:CDS
GTGTTATGGACTGGTGCCAT+TGG 0.572537 3:-29107680 MsG0380013037.01.T01:CDS
AATGTCGTATATAGTGAACT+TGG 0.574240 3:+29107513 None:intergenic
CTGGAATTCAACCAAATAGC+CGG 0.579251 3:-29107468 MsG0380013037.01.T01:CDS
CTCCTGATGAAGCATTGTAT+CGG 0.592254 3:+29107730 None:intergenic
CTGAACAATCTCTTGTGTAA+CGG 0.594307 3:+29107819 None:intergenic
ATGCGTATGTTGGAAAACTC+AGG 0.599772 3:-29107552 MsG0380013037.01.T01:CDS
AGCAATTCAGCTCATAGGAA+TGG 0.603316 3:+29107378 None:intergenic
AGACTCCCGAAGAGTGTGGA+AGG 0.609764 3:-29107798 MsG0380013037.01.T01:CDS
TCCTGTGTGTGAATCATGGA+GGG 0.622280 3:-29107842 MsG0380013037.01.T01:CDS
TCACACACAGGAGTACAAGA+AGG 0.639441 3:+29107853 None:intergenic
CATTGTATCGGAGAATCACA+AGG 0.642254 3:+29107742 None:intergenic
GTTCAGACTCCCGAAGAGTG+TGG 0.642949 3:-29107802 MsG0380013037.01.T01:CDS
GCCCTCCATGATTCACACAC+AGG 0.656658 3:+29107841 None:intergenic
AACCAAATAGCCGGTCCAGT+AGG 0.657951 3:-29107459 MsG0380013037.01.T01:CDS
TGAATTCCAGATCCGTGTGG+AGG 0.662267 3:+29107481 None:intergenic
CTCCTGTGTGTGAATCATGG+AGG 0.667171 3:-29107843 MsG0380013037.01.T01:CDS
GATACAATGCTTCATCAGGA+GGG 0.667883 3:-29107728 MsG0380013037.01.T01:CDS
ATTGACAGGCAACCTCCACA+CGG 0.696401 3:-29107493 MsG0380013037.01.T01:CDS
GGTTGAATTCCAGATCCGTG+TGG 0.703459 3:+29107478 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AATGTCGTATATAGTGAACT+TGG + Chr3:29107724-29107743 None:intergenic 30.0%
CACAGACTTAATCATATCTA+TGG - Chr3:29107632-29107651 MsG0380013037.01.T01:CDS 30.0%
CATATTTCGTTAAATCAGCT+GGG - Chr3:29107468-29107487 MsG0380013037.01.T01:CDS 30.0%
TAGTTATATGCTTGAACTCT+TGG + Chr3:29107825-29107844 None:intergenic 30.0%
TATTAGCTGCCAAAAAGATA+AGG - Chr3:29107598-29107617 MsG0380013037.01.T01:CDS 30.0%
TCATATTTCGTTAAATCAGC+TGG - Chr3:29107467-29107486 MsG0380013037.01.T01:CDS 30.0%
! AAAACTCAGGTCTAACATTT+TGG - Chr3:29107695-29107714 MsG0380013037.01.T01:CDS 30.0%
! AAACTCAGGTCTAACATTTT+GGG - Chr3:29107696-29107715 MsG0380013037.01.T01:CDS 30.0%
! TTATCAGTCTCATTTTCCAA+TGG + Chr3:29107573-29107592 None:intergenic 30.0%
AAAAAGATAAGGAAGACTGC+CGG - Chr3:29107609-29107628 MsG0380013037.01.T01:CDS 35.0%
AGATATGATTAAGTCTGTGC+CGG + Chr3:29107631-29107650 None:intergenic 35.0%
CTGAACAATCTCTTGTGTAA+CGG + Chr3:29107418-29107437 None:intergenic 35.0%
GTATCTATTGTGTTATGGAC+TGG - Chr3:29107545-29107564 MsG0380013037.01.T01:CDS 35.0%
TCACTATATACGACATTGAC+AGG - Chr3:29107727-29107746 MsG0380013037.01.T01:CDS 35.0%
TCTGCGTATCTATTGTGTTA+TGG - Chr3:29107540-29107559 MsG0380013037.01.T01:CDS 35.0%
AAAGAAATCTCCTACTGGAC+CGG + Chr3:29107788-29107807 None:intergenic 40.0%
ATGCGTATGTTGGAAAACTC+AGG - Chr3:29107682-29107701 MsG0380013037.01.T01:CDS 40.0%
CATTGTATCGGAGAATCACA+AGG + Chr3:29107495-29107514 None:intergenic 40.0%
CCAGTAGGAGATTTCTTTCT+AGG - Chr3:29107790-29107809 MsG0380013037.01.T01:CDS 40.0%
CCTAGAAAGAAATCTCCTAC+TGG + Chr3:29107793-29107812 None:intergenic 40.0%
CTGGAATTCAACCAAATAGC+CGG - Chr3:29107766-29107785 MsG0380013037.01.T01:CDS 40.0%
GATACAATGCTTCATCAGGA+GGG - Chr3:29107506-29107525 MsG0380013037.01.T01:CDS 40.0%
TAGGAGATTTCTTTCTAGGC+AGG - Chr3:29107794-29107813 MsG0380013037.01.T01:CDS 40.0%
! GGCAGTCTTCCTTATCTTTT+TGG + Chr3:29107610-29107629 None:intergenic 40.0%
! TTTTCCAACATACGCATTGC+TGG + Chr3:29107679-29107698 None:intergenic 40.0%
!! CTCCTGATGAAGCATTGTAT+CGG + Chr3:29107507-29107526 None:intergenic 40.0%
CGATACAATGCTTCATCAGG+AGG - Chr3:29107505-29107524 MsG0380013037.01.T01:CDS 45.0%
CTCCGATACAATGCTTCATC+AGG - Chr3:29107502-29107521 MsG0380013037.01.T01:CDS 45.0%
GTACTCCTGTGTGTGAATCA+TGG - Chr3:29107388-29107407 MsG0380013037.01.T01:CDS 45.0%
TCCTGTGTGTGAATCATGGA+GGG - Chr3:29107392-29107411 MsG0380013037.01.T01:CDS 45.0%
AACCAAATAGCCGGTCCAGT+AGG - Chr3:29107775-29107794 MsG0380013037.01.T01:CDS 50.0%
ATTGACAGGCAACCTCCACA+CGG - Chr3:29107741-29107760 MsG0380013037.01.T01:CDS 50.0%
CGTCAACCTTCCACACTCTT+CGG + Chr3:29107445-29107464 None:intergenic 50.0%
CGTTCCAGCAATGCGTATGT+TGG - Chr3:29107672-29107691 MsG0380013037.01.T01:CDS 50.0%
CTCCTGTGTGTGAATCATGG+AGG - Chr3:29107391-29107410 MsG0380013037.01.T01:CDS 50.0%
GGTTGAATTCCAGATCCGTG+TGG + Chr3:29107759-29107778 None:intergenic 50.0%
GTCAACCTTCCACACTCTTC+GGG + Chr3:29107444-29107463 None:intergenic 50.0%
TGAATTCCAGATCCGTGTGG+AGG + Chr3:29107756-29107775 None:intergenic 50.0%
!! GTGTTATGGACTGGTGCCAT+TGG - Chr3:29107554-29107573 MsG0380013037.01.T01:CDS 50.0%
CTCCTACTGGACCGGCTATT+TGG + Chr3:29107780-29107799 None:intergenic 55.0%
GCCCTCCATGATTCACACAC+AGG + Chr3:29107396-29107415 None:intergenic 55.0%
GTTCAGACTCCCGAAGAGTG+TGG - Chr3:29107432-29107451 MsG0380013037.01.T01:CDS 55.0%
! AGACTCCCGAAGAGTGTGGA+AGG - Chr3:29107436-29107455 MsG0380013037.01.T01:CDS 55.0%
AGGCAACCTCCACACGGATC+TGG - Chr3:29107747-29107766 MsG0380013037.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 29107384 29107872 29107384 ID=MsG0380013037.01;Name=MsG0380013037.01
Chr3 mRNA 29107384 29107872 29107384 ID=MsG0380013037.01.T01;Parent=MsG0380013037.01;Name=MsG0380013037.01.T01;_AED=0.18;_eAED=0.18;_QI=0|-1|0|1|-1|0|1|0|162
Chr3 exon 29107384 29107872 29107384 ID=MsG0380013037.01.T01:exon:7196;Parent=MsG0380013037.01.T01
Chr3 CDS 29107384 29107872 29107384 ID=MsG0380013037.01.T01:cds;Parent=MsG0380013037.01.T01
Chr3 mRNA 29107384 29107860 29107384 ID=MsG0380013037.01.T02;Parent=MsG0380013037.01;Name=MsG0380013037.01.T02;_AED=0.19;_eAED=0.19;_QI=0|-1|0|1|-1|0|1|0|158
Chr3 exon 29107384 29107860 29107384 ID=MsG0380013037.01.T02:exon:7197;Parent=MsG0380013037.01.T02
Chr3 CDS 29107384 29107860 29107384 ID=MsG0380013037.01.T02:cds;Parent=MsG0380013037.01.T02
Gene Sequence

>MsG0380013037.01.T02

GTGTGTGAATCATGGAGGGCCGTTACACAAGAGATTGTTCAGACTCCCGAAGAGTGTGGAAGGTTGACGTTTCATATTTCGTTAAATCAGCTGGGTCCTTGTGATTCTCCGATACAATGCTTCATCAGGAGGGATAGAGAAACTTCTGCGTATCTATTGTGTTATGGACTGGTGCCATTGGAAAATGAGACTGATAAGATGTTATTAGCTGCCAAAAAGATAAGGAAGACTGCCGGCACAGACTTAATCATATCTATGGTTGCAGTCGATTTTTCTCGTTCCAGCAATGCGTATGTTGGAAAACTCAGGTCTAACATTTTGGGAACCAAGTTCACTATATACGACATTGACAGGCAACCTCCACACGGATCTGGAATTCAACCAAATAGCCGGTCCAGTAGGAGATTTCTTTCTAGGCAGGTGTCTCCAAGAGTTCAAGCATATAACTATATTGTTAGCACCATTCCTATGAGCTGA

>MsG0380013037.01.T01

TCTTGTACTCCTGTGTGTGAATCATGGAGGGCCGTTACACAAGAGATTGTTCAGACTCCCGAAGAGTGTGGAAGGTTGACGTTTCATATTTCGTTAAATCAGCTGGGTCCTTGTGATTCTCCGATACAATGCTTCATCAGGAGGGATAGAGAAACTTCTGCGTATCTATTGTGTTATGGACTGGTGCCATTGGAAAATGAGACTGATAAGATGTTATTAGCTGCCAAAAAGATAAGGAAGACTGCCGGCACAGACTTAATCATATCTATGGTTGCAGTCGATTTTTCTCGTTCCAGCAATGCGTATGTTGGAAAACTCAGGTCTAACATTTTGGGAACCAAGTTCACTATATACGACATTGACAGGCAACCTCCACACGGATCTGGAATTCAACCAAATAGCCGGTCCAGTAGGAGATTTCTTTCTAGGCAGGTGTCTCCAAGAGTTCAAGCATATAACTATATTGTTAGCACCATTCCTATGAGCTGA

Protein sequence

>MsG0380013037.01.T02

VCESWRAVTQEIVQTPEECGRLTFHISLNQLGPCDSPIQCFIRRDRETSAYLLCYGLVPLENETDKMLLAAKKIRKTAGTDLIISMVAVDFSRSSNAYVGKLRSNILGTKFTIYDIDRQPPHGSGIQPNSRSSRRFLSRQVSPRVQAYNYIVSTIPMS*

>MsG0380013037.01.T01

SCTPVCESWRAVTQEIVQTPEECGRLTFHISLNQLGPCDSPIQCFIRRDRETSAYLLCYGLVPLENETDKMLLAAKKIRKTAGTDLIISMVAVDFSRSSNAYVGKLRSNILGTKFTIYDIDRQPPHGSGIQPNSRSSRRFLSRQVSPRVQAYNYIVSTIPMS*