AlfalfaGEDB | MsG0380013037.01

M. sativa cultivar ZhongmuNo.1 / MsG0380013037.01

KEGG: K19600
GO: -
PFAM: PF01167

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013037.01.T01	XP_003612033.1	83.019	159	25	1	1	159	85	241	4.45E-85	266
MsG0380013037.01.T02	XP_003612033.1	83.871	155	23	1	1	155	89	241	8.74E-84	262

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013037.01.T01	Q6Z2G9	56.875	160	65	3	1	159	98	254	5.86E-54	179
MsG0380013037.01.T02	Q6Z2G9	57.692	156	62	3	1	155	102	254	6.27E-53	176

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013037.01.T01	G7KEX1	83.019	159	25	1	1	159	85	241	2.12e-85	266
MsG0380013037.01.T02	G7KEX1	83.871	155	23	1	1	155	89	241	4.17e-84	262

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TFs/TRs:

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Gene ID	Type	Classification
MsG0380013037.01.T01	TF	TUB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013037.01.T01	MTR_5g020580	83.019	159	25	1	1	159	85	241	5.39e-89	266
MsG0380013037.01.T01	MTR_8g077195	62.025	158	58	1	2	159	84	239	1.02e-65	206
MsG0380013037.01.T01	MTR_8g077620	55.975	159	63	3	2	159	83	235	4.54e-53	173
MsG0380013037.01.T01	MTR_8g077675	53.459	159	68	3	2	159	79	232	6.85e-52	170
MsG0380013037.01.T01	MTR_1g022370	50.000	162	76	3	1	159	100	259	1.64e-46	157
MsG0380013037.01.T01	MTR_2g020280	50.943	159	76	1	1	159	69	225	4.67e-44	149
MsG0380013037.01.T01	MTR_1g098790	49.689	161	76	4	1	159	83	240	3.27e-42	145
MsG0380013037.01.T01	MTR_2g007930	50.307	163	74	5	1	159	84	243	1.89e-41	143
MsG0380013037.01.T01	MTR_2g076200	45.625	160	83	3	1	159	72	228	2.91e-39	136
MsG0380013037.01.T01	MTR_1g088960	47.403	154	78	2	1	154	80	230	1.16e-36	130

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380013037.01.T01	AT2G18280	54.717	159	67	2	1	159	78	231	2.04e-53	174
MsG0380013037.01.T01	AT2G18280	54.717	159	67	2	1	159	78	231	2.04e-53	174
MsG0380013037.01.T01	AT1G47270	48.667	150	66	3	1	149	74	213	5.49e-42	144
MsG0380013037.01.T01	AT1G47270	48.667	150	66	3	1	149	98	237	7.41e-42	144
MsG0380013037.01.T01	AT1G47270	48.667	150	66	3	1	149	99	238	7.87e-42	144
MsG0380013037.01.T01	AT1G76900	49.390	164	75	4	1	159	87	247	1.07e-41	145
MsG0380013037.01.T01	AT1G76900	49.390	164	75	4	1	159	87	247	1.07e-41	145
MsG0380013037.01.T01	AT1G76900	49.390	164	75	4	1	159	87	247	1.07e-41	145
MsG0380013037.01.T01	AT1G25280	51.553	161	76	2	1	159	89	249	2.70e-40	140
MsG0380013037.01.T01	AT2G47900	48.447	161	79	2	1	159	82	240	3.15e-40	140

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Find 41 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTCAGGTCTAACATTT+TGG	0.098131	3:-29107539	MsG0380013037.01.T01:CDS
AAAGAAATCTCCTACTGGAC+CGG	0.552476	3:+29107449	None:intergenic
AACCAAATAGCCGGTCCAGT+AGG	0.657951	3:-29107459	MsG0380013037.01.T01:CDS
AATGTCGTATATAGTGAACT+TGG	0.574240	3:+29107513	None:intergenic
AGACTCCCGAAGAGTGTGGA+AGG	0.609764	3:-29107798	MsG0380013037.01.T01:CDS
AGATATGATTAAGTCTGTGC+CGG	0.566788	3:+29107606	None:intergenic
AGCAATTCAGCTCATAGGAA+TGG	0.603316	3:+29107378	None:intergenic
AGGCAACCTCCACACGGATC+TGG	0.416160	3:-29107487	MsG0380013037.01.T01:CDS
ATAAGAGCAATTCAGCTCAT+AGG	0.461752	3:+29107373	None:intergenic
ATGCGTATGTTGGAAAACTC+AGG	0.599772	3:-29107552	MsG0380013037.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATGTCGTATATAGTGAACT+TGG	+	Chr3:29107724-29107743	None:intergenic	30.0%
CACAGACTTAATCATATCTA+TGG	-	Chr3:29107632-29107651	MsG0380013037.01.T01:CDS	30.0%
CATATTTCGTTAAATCAGCT+GGG	-	Chr3:29107468-29107487	MsG0380013037.01.T01:CDS	30.0%
TAGTTATATGCTTGAACTCT+TGG	+	Chr3:29107825-29107844	None:intergenic	30.0%
TATTAGCTGCCAAAAAGATA+AGG	-	Chr3:29107598-29107617	MsG0380013037.01.T01:CDS	30.0%
TCATATTTCGTTAAATCAGC+TGG	-	Chr3:29107467-29107486	MsG0380013037.01.T01:CDS	30.0%
AAAAAGATAAGGAAGACTGC+CGG	-	Chr3:29107609-29107628	MsG0380013037.01.T01:CDS	35.0%
AGATATGATTAAGTCTGTGC+CGG	+	Chr3:29107631-29107650	None:intergenic	35.0%
CTGAACAATCTCTTGTGTAA+CGG	+	Chr3:29107418-29107437	None:intergenic	35.0%
GTATCTATTGTGTTATGGAC+TGG	-	Chr3:29107545-29107564	MsG0380013037.01.T01:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	29107384	29107872	29107384	ID=MsG0380013037.01;Name=MsG0380013037.01
Chr3	mRNA	29107384	29107872	29107384	ID=MsG0380013037.01.T01;Parent=MsG0380013037.01;Name=MsG0380013037.01.T01;_AED=0.18;_eAED=0.18;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|162
Chr3	exon	29107384	29107872	29107384	ID=MsG0380013037.01.T01:exon:7196;Parent=MsG0380013037.01.T01
Chr3	CDS	29107384	29107872	29107384	ID=MsG0380013037.01.T01:cds;Parent=MsG0380013037.01.T01
Chr3	mRNA	29107384	29107860	29107384	ID=MsG0380013037.01.T02;Parent=MsG0380013037.01;Name=MsG0380013037.01.T02;_AED=0.19;_eAED=0.19;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|158
Chr3	exon	29107384	29107860	29107384	ID=MsG0380013037.01.T02:exon:7197;Parent=MsG0380013037.01.T02
Chr3	CDS	29107384	29107860	29107384	ID=MsG0380013037.01.T02:cds;Parent=MsG0380013037.01.T02

Gene Sequence

>MsG0380013037.01.T02

GTGTGTGAATCATGGAGGGCCGTTACACAAGAGATTGTTCAGACTCCCGAAGAGTGTGGAAGGTTGACGTTTCATATTTCGTTAAATCAGCTGGGTCCTTGTGATTCTCCGATACAATGCTTCATCAGGAGGGATAGAGAAACTTCTGCGTATCTATTGTGTTATGGACTGGTGCCATTGGAAAATGAGACTGATAAGATGTTATTAGCTGCCAAAAAGATAAGGAAGACTGCCGGCACAGACTTAATCATATCTATGGTTGCAGTCGATTTTTCTCGTTCCAGCAATGCGTATGTTGGAAAACTCAGGTCTAACATTTTGGGAACCAAGTTCACTATATACGACATTGACAGGCAACCTCCACACGGATCTGGAATTCAACCAAATAGCCGGTCCAGTAGGAGATTTCTTTCTAGGCAGGTGTCTCCAAGAGTTCAAGCATATAACTATATTGTTAGCACCATTCCTATGAGCTGA

>MsG0380013037.01.T01

TCTTGTACTCCTGTGTGTGAATCATGGAGGGCCGTTACACAAGAGATTGTTCAGACTCCCGAAGAGTGTGGAAGGTTGACGTTTCATATTTCGTTAAATCAGCTGGGTCCTTGTGATTCTCCGATACAATGCTTCATCAGGAGGGATAGAGAAACTTCTGCGTATCTATTGTGTTATGGACTGGTGCCATTGGAAAATGAGACTGATAAGATGTTATTAGCTGCCAAAAAGATAAGGAAGACTGCCGGCACAGACTTAATCATATCTATGGTTGCAGTCGATTTTTCTCGTTCCAGCAATGCGTATGTTGGAAAACTCAGGTCTAACATTTTGGGAACCAAGTTCACTATATACGACATTGACAGGCAACCTCCACACGGATCTGGAATTCAACCAAATAGCCGGTCCAGTAGGAGATTTCTTTCTAGGCAGGTGTCTCCAAGAGTTCAAGCATATAACTATATTGTTAGCACCATTCCTATGAGCTGA

Protein sequence

>MsG0380013037.01.T02

VCESWRAVTQEIVQTPEECGRLTFHISLNQLGPCDSPIQCFIRRDRETSAYLLCYGLVPLENETDKMLLAAKKIRKTAGTDLIISMVAVDFSRSSNAYVGKLRSNILGTKFTIYDIDRQPPHGSGIQPNSRSSRRFLSRQVSPRVQAYNYIVSTIPMS*

>MsG0380013037.01.T01

SCTPVCESWRAVTQEIVQTPEECGRLTFHISLNQLGPCDSPIQCFIRRDRETSAYLLCYGLVPLENETDKMLLAAKKIRKTAGTDLIISMVAVDFSRSSNAYVGKLRSNILGTKFTIYDIDRQPPHGSGIQPNSRSSRRFLSRQVSPRVQAYNYIVSTIPMS*