Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013130.01.T01 | XP_027339360.1 | 84.286 | 70 | 10 | 1 | 1 | 69 | 1 | 70 | 2.50E-33 | 120 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013130.01.T01 | Q9LJW5 | 60.759 | 79 | 21 | 2 | 1 | 69 | 1 | 79 | 1.12E-23 | 88.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013130.01.T01 | A0A151RXL6 | 81.429 | 70 | 12 | 1 | 1 | 69 | 1 | 70 | 4.27e-32 | 115 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0380013130.01.T01 | TF | zf-HD |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013130.01.T01 | MTR_3g039810 | 81.159 | 69 | 9 | 1 | 1 | 69 | 1 | 65 | 8.81e-34 | 111 |
| MsG0380013130.01.T01 | MTR_6g012220 | 63.768 | 69 | 18 | 2 | 1 | 69 | 1 | 62 | 4.83e-25 | 89.0 |
| MsG0380013130.01.T01 | MTR_3g100650 | 68.750 | 48 | 15 | 0 | 22 | 69 | 78 | 125 | 9.79e-17 | 72.0 |
| MsG0380013130.01.T01 | MTR_5g065440 | 63.265 | 49 | 18 | 0 | 21 | 69 | 55 | 103 | 9.17e-15 | 66.6 |
| MsG0380013130.01.T01 | MTR_5g020510 | 53.704 | 54 | 25 | 0 | 16 | 69 | 91 | 144 | 6.54e-14 | 64.7 |
| MsG0380013130.01.T01 | MTR_1g077020 | 58.333 | 48 | 20 | 0 | 22 | 69 | 7 | 54 | 1.13e-13 | 62.8 |
| MsG0380013130.01.T01 | MTR_2g076780 | 54.386 | 57 | 24 | 1 | 13 | 69 | 28 | 82 | 1.67e-13 | 62.8 |
| MsG0380013130.01.T01 | MTR_5g032600 | 61.702 | 47 | 18 | 0 | 23 | 69 | 77 | 123 | 3.04e-13 | 62.4 |
| MsG0380013130.01.T01 | MTR_8g069080 | 57.447 | 47 | 20 | 0 | 23 | 69 | 68 | 114 | 1.48e-12 | 60.5 |
| MsG0380013130.01.T01 | MTR_4g134580 | 61.702 | 47 | 18 | 0 | 23 | 69 | 112 | 158 | 1.62e-12 | 60.8 |
| MsG0380013130.01.T01 | MTR_7g010300 | 56.000 | 50 | 20 | 1 | 22 | 69 | 86 | 135 | 5.37e-12 | 59.3 |
| MsG0380013130.01.T01 | MTR_6g073013 | 50.980 | 51 | 24 | 1 | 20 | 69 | 15 | 65 | 3.45e-11 | 55.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380013130.01.T01 | AT3G28917 | 60.759 | 79 | 21 | 2 | 1 | 69 | 1 | 79 | 1.14e-24 | 88.6 |
| MsG0380013130.01.T01 | AT1G18835 | 64.789 | 71 | 20 | 2 | 1 | 69 | 1 | 68 | 1.42e-23 | 85.5 |
| MsG0380013130.01.T01 | AT1G74660 | 54.217 | 83 | 24 | 1 | 1 | 69 | 2 | 84 | 4.14e-23 | 84.7 |
| MsG0380013130.01.T01 | AT1G74660 | 54.217 | 83 | 24 | 1 | 1 | 69 | 2 | 84 | 4.17e-23 | 84.7 |
| MsG0380013130.01.T01 | AT4G24660 | 70.833 | 48 | 14 | 0 | 22 | 69 | 47 | 94 | 4.19e-17 | 72.4 |
| MsG0380013130.01.T01 | AT4G24660 | 70.833 | 48 | 14 | 0 | 22 | 69 | 47 | 94 | 6.63e-17 | 72.0 |
| MsG0380013130.01.T01 | AT3G50890 | 52.381 | 63 | 30 | 0 | 7 | 69 | 43 | 105 | 1.68e-16 | 71.2 |
| MsG0380013130.01.T01 | AT2G18350 | 65.957 | 47 | 16 | 0 | 23 | 69 | 81 | 127 | 2.58e-15 | 68.2 |
| MsG0380013130.01.T01 | AT2G02540 | 60.417 | 48 | 19 | 0 | 22 | 69 | 85 | 132 | 1.77e-14 | 66.2 |
| MsG0380013130.01.T01 | AT2G02540 | 60.417 | 48 | 19 | 0 | 22 | 69 | 85 | 132 | 1.77e-14 | 66.2 |
| MsG0380013130.01.T01 | AT5G65410 | 64.583 | 48 | 17 | 0 | 22 | 69 | 73 | 120 | 4.06e-14 | 65.1 |
| MsG0380013130.01.T01 | AT1G14440 | 60.417 | 48 | 19 | 0 | 22 | 69 | 88 | 135 | 6.05e-14 | 64.7 |
| MsG0380013130.01.T01 | AT1G14440 | 60.417 | 48 | 19 | 0 | 22 | 69 | 88 | 135 | 6.05e-14 | 64.7 |
| MsG0380013130.01.T01 | AT1G75240 | 62.500 | 48 | 18 | 0 | 22 | 69 | 74 | 121 | 8.83e-14 | 64.3 |
| MsG0380013130.01.T01 | AT5G15210 | 58.333 | 48 | 18 | 1 | 24 | 69 | 56 | 103 | 4.52e-12 | 59.3 |
| MsG0380013130.01.T01 | AT5G42780 | 55.769 | 52 | 21 | 1 | 18 | 69 | 58 | 107 | 6.08e-12 | 58.9 |
| MsG0380013130.01.T01 | AT1G69600 | 56.250 | 48 | 19 | 1 | 24 | 69 | 31 | 78 | 1.17e-11 | 58.2 |
| MsG0380013130.01.T01 | AT1G14687 | 52.174 | 46 | 17 | 2 | 24 | 69 | 7 | 47 | 3.28e-11 | 55.8 |
Find 20 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAATCATGCAGCAAATAT+TGG | 0.303529 | 3:-30962149 | MsG0380013130.01.T01:CDS |
| CACTTGTTCCTTCTTCTGTA+TGG | 0.308369 | 3:+30962090 | None:intergenic |
| ATTACAAGAAATATTCGATA+TGG | 0.316447 | 3:-30962182 | MsG0380013130.01.T01:CDS |
| CGAATATTTCTTGTAATTGA+AGG | 0.364064 | 3:+30962188 | None:intergenic |
| GGTATGCTGTAGATGGTTGC+AGG | 0.408709 | 3:-30962124 | MsG0380013130.01.T01:CDS |
| GTGCTGCTTGTGGTTGTCAT+AGG | 0.423325 | 3:-30962055 | MsG0380013130.01.T01:CDS |
| TTCCTTCTTCTGTATGGCCA+TGG | 0.447705 | 3:+30962096 | None:intergenic |
| TGTCATAGGACTTTCATAGA+AGG | 0.452722 | 3:-30962041 | MsG0380013130.01.T01:CDS |
| AGTTGTGGTGAAAAGAGATA+TGG | 0.462976 | 3:-30962219 | MsG0380013130.01.T01:CDS |
| ATTGGAGGGTATGCTGTAGA+TGG | 0.520125 | 3:-30962131 | MsG0380013130.01.T01:CDS |
| TTCCATGGCCATACAGAAGA+AGG | 0.528223 | 3:-30962098 | MsG0380013130.01.T01:CDS |
| GCTCTTACATGTGCTGCTTG+TGG | 0.547604 | 3:-30962065 | MsG0380013130.01.T01:CDS |
| GATGGTTGCAGGGAGTTCCA+TGG | 0.568156 | 3:-30962113 | MsG0380013130.01.T01:CDS |
| AATCATGCAGCAAATATTGG+AGG | 0.572292 | 3:-30962146 | MsG0380013130.01.T01:CDS |
| GATGAAGAAGAGACAAGTTG+TGG | 0.602168 | 3:-30962234 | MsG0380013130.01.T01:CDS |
| ATCATGCAGCAAATATTGGA+GGG | 0.624158 | 3:-30962145 | MsG0380013130.01.T01:CDS |
| GTATGCTGTAGATGGTTGCA+GGG | 0.640365 | 3:-30962123 | MsG0380013130.01.T01:CDS |
| CATACAGAAGAAGGAACAAG+TGG | 0.640403 | 3:-30962089 | MsG0380013130.01.T01:CDS |
| ATACAGAAGAAGGAACAAGT+GGG | 0.687253 | 3:-30962088 | MsG0380013130.01.T01:CDS |
| TACAGAAGAAGGAACAAGTG+GGG | 0.703673 | 3:-30962087 | MsG0380013130.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTACAAGAAATATTCGATA+TGG | - | Chr3:30962079-30962098 | MsG0380013130.01.T01:CDS | 20.0% |
| ! | CGAATATTTCTTGTAATTGA+AGG | + | Chr3:30962076-30962095 | None:intergenic | 25.0% |
| AAGAATCATGCAGCAAATAT+TGG | - | Chr3:30962112-30962131 | MsG0380013130.01.T01:CDS | 30.0% | |
| AATCATGCAGCAAATATTGG+AGG | - | Chr3:30962115-30962134 | MsG0380013130.01.T01:CDS | 35.0% | |
| AGTTGTGGTGAAAAGAGATA+TGG | - | Chr3:30962042-30962061 | MsG0380013130.01.T01:CDS | 35.0% | |
| ATACAGAAGAAGGAACAAGT+GGG | - | Chr3:30962173-30962192 | MsG0380013130.01.T01:CDS | 35.0% | |
| ATCATGCAGCAAATATTGGA+GGG | - | Chr3:30962116-30962135 | MsG0380013130.01.T01:CDS | 35.0% | |
| TGTCATAGGACTTTCATAGA+AGG | - | Chr3:30962220-30962239 | MsG0380013130.01.T01:CDS | 35.0% | |
| CACTTGTTCCTTCTTCTGTA+TGG | + | Chr3:30962174-30962193 | None:intergenic | 40.0% | |
| CATACAGAAGAAGGAACAAG+TGG | - | Chr3:30962172-30962191 | MsG0380013130.01.T01:CDS | 40.0% | |
| TACAGAAGAAGGAACAAGTG+GGG | - | Chr3:30962174-30962193 | MsG0380013130.01.T01:CDS | 40.0% | |
| GTATGCTGTAGATGGTTGCA+GGG | - | Chr3:30962138-30962157 | MsG0380013130.01.T01:CDS | 45.0% | |
| TTCCATGGCCATACAGAAGA+AGG | - | Chr3:30962163-30962182 | MsG0380013130.01.T01:CDS | 45.0% | |
| TTCCTTCTTCTGTATGGCCA+TGG | + | Chr3:30962168-30962187 | None:intergenic | 45.0% | |
| ! | ATTGGAGGGTATGCTGTAGA+TGG | - | Chr3:30962130-30962149 | MsG0380013130.01.T01:CDS | 45.0% |
| GGTATGCTGTAGATGGTTGC+AGG | - | Chr3:30962137-30962156 | MsG0380013130.01.T01:CDS | 50.0% | |
| !! | GCTCTTACATGTGCTGCTTG+TGG | - | Chr3:30962196-30962215 | MsG0380013130.01.T01:CDS | 50.0% |
| !! | GTGCTGCTTGTGGTTGTCAT+AGG | - | Chr3:30962206-30962225 | MsG0380013130.01.T01:CDS | 50.0% |
| GATGGTTGCAGGGAGTTCCA+TGG | - | Chr3:30962148-30962167 | MsG0380013130.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 30962028 | 30962255 | 30962028 | ID=MsG0380013130.01;Name=MsG0380013130.01 |
| Chr3 | mRNA | 30962028 | 30962255 | 30962028 | ID=MsG0380013130.01.T01;Parent=MsG0380013130.01;Name=MsG0380013130.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|75 |
| Chr3 | exon | 30962028 | 30962255 | 30962028 | ID=MsG0380013130.01.T01:exon:6454;Parent=MsG0380013130.01.T01 |
| Chr3 | CDS | 30962028 | 30962255 | 30962028 | ID=MsG0380013130.01.T01:cds;Parent=MsG0380013130.01.T01 |
| Gene Sequence |
| Protein sequence |
