AlfalfaGEDB | MsG0380014546.01

M. sativa cultivar ZhongmuNo.1 / MsG0380014546.01

KEGG: K08065
GO: GO:0046982
PFAM: PF00808

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014546.01.T03	XP_004516113.2	47.126	174	84	1	1	174	66	231	8.52E-41	147
MsG0380014546.01.T01	RHN67563.1	46	100	54	0	1	100	1	100	4.69E-19	87
MsG0380014546.01.T02	XP_004516113.2	53.125	192	88	1	3	192	40	231	8.09E-57	189

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014546.01.T02	P63139	39.423	104	62	1	1	103	39	142	2.89E-21	89.7
MsG0380014546.01.T03	Q75IZ7	38.318	107	57	2	1	106	38	136	8.07E-17	77.8

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014546.01.T01	A0A396ISU1	46.000	100	54	0	1	100	1	100	2.24e-19	87.0
MsG0380014546.01.T02	A0A1S2Z6W5	53.125	192	88	1	3	192	40	231	3.86e-57	189
MsG0380014546.01.T03	A0A1S2Z6W5	47.126	174	84	1	1	174	66	231	4.07e-41	147

TFs/TRs:

Gene ID	Type	Classification
MsG0380014546.01.T02	TF	NF-YB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014546.01.T03	MTR_5g095740	45.333	75	41	0	1	75	37	111	5.64e-18	77.4
MsG0380014546.01.T03	MTR_3g058980	42.857	91	49	1	1	91	41	128	1.10e-17	76.6
MsG0380014546.01.T03	MTR_4g133952	44.000	75	42	0	1	75	72	146	6.82e-17	75.9
MsG0380014546.01.T03	MTR_1g039040	34.545	110	70	2	1	108	20	129	7.01e-17	74.7
MsG0380014546.01.T03	MTR_8g093920	44.000	75	42	0	1	75	54	128	8.03e-17	75.1
MsG0380014546.01.T03	MTR_1g083070	38.961	77	47	0	1	77	18	94	9.86e-17	72.8
MsG0380014546.01.T03	MTR_7g061270	41.333	75	44	0	1	75	40	114	1.18e-16	73.6
MsG0380014546.01.T03	MTR_7g100650	41.333	75	44	0	1	75	40	114	1.25e-16	73.6
MsG0380014546.01.T03	MTR_2g026710	42.667	75	43	0	1	75	31	105	1.31e-16	73.6
MsG0380014546.01.T03	MTR_8g091720	42.667	75	43	0	1	75	42	116	1.38e-16	74.3
MsG0380014546.01.T03	MTR_1g088860	40.000	75	45	0	1	75	50	124	2.78e-16	73.2
MsG0380014546.01.T03	MTR_1g072790	40.000	75	45	0	1	75	43	117	7.28e-16	71.6
MsG0380014546.01.T03	MTR_0392s0020	37.333	75	47	0	1	75	32	106	1.00e-14	67.8
MsG0380014546.01.T03	MTR_4g133938	40.000	75	45	0	1	75	33	107	1.07e-14	67.8
MsG0380014546.01.T03	MTR_4g119500	37.333	75	47	0	1	75	36	110	1.13e-14	67.4
MsG0380014546.01.T03	MTR_5g095900	33.333	81	54	0	1	81	30	110	9.47e-14	66.2
MsG0380014546.01.T03	MTR_3g462720	50.000	56	28	0	98	153	20	75	1.24e-11	58.5
MsG0380014546.01.T03	MTR_1g029070	34.667	75	49	0	1	75	66	140	1.32e-11	60.5
MsG0380014546.01.T01	MTR_3g462720	43.836	73	41	0	28	100	3	75	4.42e-13	60.8
MsG0380014546.01.T02	MTR_5g095740	44.681	94	51	1	11	103	18	111	2.31e-22	89.4
MsG0380014546.01.T02	MTR_3g058980	40.517	116	65	2	5	119	16	128	5.01e-22	88.6
MsG0380014546.01.T02	MTR_1g083070	42.529	87	50	0	19	105	8	94	9.87e-22	86.3
MsG0380014546.01.T02	MTR_2g026710	41.000	100	58	1	5	103	6	105	1.15e-21	87.0
MsG0380014546.01.T02	MTR_7g061270	41.053	95	55	1	10	103	20	114	2.65e-21	86.3
MsG0380014546.01.T02	MTR_7g100650	41.053	95	55	1	10	103	20	114	2.68e-21	86.3
MsG0380014546.01.T02	MTR_1g039040	36.220	127	78	2	13	138	3	127	3.92e-21	86.3
MsG0380014546.01.T02	MTR_8g093920	46.429	84	45	0	20	103	45	128	6.72e-21	86.3
MsG0380014546.01.T02	MTR_8g091720	45.238	84	46	0	20	103	33	116	9.57e-21	85.5
MsG0380014546.01.T02	MTR_1g088860	42.857	84	48	0	20	103	41	124	1.46e-20	85.1
MsG0380014546.01.T02	MTR_4g133952	45.238	84	46	0	20	103	63	146	2.41e-20	85.5
MsG0380014546.01.T02	MTR_1g072790	41.667	84	49	0	20	103	34	117	5.98e-20	82.8
MsG0380014546.01.T02	MTR_4g133938	42.857	84	48	0	20	103	24	107	4.51e-19	79.7
MsG0380014546.01.T02	MTR_0392s0020	40.476	84	50	0	20	103	23	106	4.69e-19	79.7
MsG0380014546.01.T02	MTR_4g119500	40.476	84	50	0	20	103	27	110	5.16e-19	79.3
MsG0380014546.01.T02	MTR_5g095900	34.066	91	60	0	19	109	20	110	7.25e-17	75.1
MsG0380014546.01.T02	MTR_1g029070	36.905	84	53	0	20	103	57	140	1.78e-15	71.2
MsG0380014546.01.T02	MTR_3g462720	48.387	62	32	0	110	171	14	75	2.48e-13	63.5
MsG0380014546.01.T02	MTR_1g029100	35.802	81	52	0	20	100	7	87	1.75e-12	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014546.01.T03	AT2G38880	39.286	84	51	0	1	84	35	118	3.98e-18	76.6
MsG0380014546.01.T03	AT4G14540	44.000	75	42	0	1	75	35	109	7.79e-18	76.6
MsG0380014546.01.T03	AT2G38880	41.333	75	44	0	1	75	35	109	1.40e-17	75.5
MsG0380014546.01.T03	AT2G38880	41.333	75	44	0	1	75	35	109	1.40e-17	75.5
MsG0380014546.01.T03	AT2G38880	41.333	75	44	0	1	75	35	109	1.40e-17	75.5
MsG0380014546.01.T03	AT2G38880	41.333	75	44	0	1	75	35	109	1.75e-17	75.1
MsG0380014546.01.T03	AT2G38880	41.333	75	44	0	1	75	35	109	1.97e-17	74.3
MsG0380014546.01.T03	AT2G38880	41.333	75	44	0	1	75	35	109	2.05e-17	75.5
MsG0380014546.01.T03	AT2G38880	41.333	75	44	0	1	75	35	109	2.05e-17	75.5
MsG0380014546.01.T03	AT1G21970	36.975	119	69	2	1	113	73	191	8.35e-17	75.5
MsG0380014546.01.T03	AT5G47640	44.000	75	42	0	1	75	41	115	9.85e-17	74.3
MsG0380014546.01.T03	AT2G37060	42.667	75	43	0	1	75	44	118	1.72e-16	73.2
MsG0380014546.01.T03	AT2G37060	42.667	75	43	0	1	75	44	118	1.72e-16	73.2
MsG0380014546.01.T03	AT2G37060	42.667	75	43	0	1	75	44	118	1.72e-16	73.2
MsG0380014546.01.T03	AT2G38880	41.333	75	44	0	1	75	35	109	1.74e-16	72.8
MsG0380014546.01.T03	AT2G38880	41.333	75	44	0	1	75	35	109	1.74e-16	72.8
MsG0380014546.01.T03	AT2G38880	41.333	75	44	0	1	75	35	109	1.74e-16	72.8
MsG0380014546.01.T03	AT2G13570	44.000	75	42	0	1	75	50	124	3.99e-16	73.2
MsG0380014546.01.T03	AT1G09030	38.667	75	46	0	1	75	17	91	6.83e-16	70.9
MsG0380014546.01.T03	AT5G47670	40.000	75	45	0	1	75	43	117	5.42e-15	70.1
MsG0380014546.01.T03	AT2G47810	37.333	75	47	0	1	75	65	139	6.52e-15	68.9
MsG0380014546.01.T03	AT5G47670	40.000	75	45	0	1	75	72	146	7.98e-15	70.1
MsG0380014546.01.T03	AT5G47670	40.000	75	45	0	1	75	72	146	7.98e-15	70.1
MsG0380014546.01.T03	AT3G53340	40.000	75	45	0	1	75	43	117	8.51e-15	68.9
MsG0380014546.01.T03	AT3G53340	40.000	75	45	0	1	75	43	117	8.51e-15	68.9
MsG0380014546.01.T03	AT3G53340	40.000	75	45	0	1	75	43	117	1.25e-14	67.4
MsG0380014546.01.T03	AT3G53340	40.000	75	45	0	1	75	43	117	3.23e-14	67.0
MsG0380014546.01.T03	AT3G53340	40.000	75	45	0	1	75	43	117	3.23e-14	67.0
MsG0380014546.01.T02	AT2G38880	36.607	112	70	1	2	112	7	118	4.05e-23	90.1
MsG0380014546.01.T02	AT2G38880	37.864	103	63	1	2	103	7	109	1.26e-22	89.0
MsG0380014546.01.T02	AT2G38880	37.864	103	63	1	2	103	7	109	1.26e-22	89.0
MsG0380014546.01.T02	AT2G38880	37.864	103	63	1	2	103	7	109	1.26e-22	89.0
MsG0380014546.01.T02	AT2G38880	37.864	103	63	1	2	103	7	109	1.65e-22	88.6
MsG0380014546.01.T02	AT2G38880	37.864	103	63	1	2	103	7	109	2.56e-22	89.0
MsG0380014546.01.T02	AT2G38880	37.864	103	63	1	2	103	7	109	2.56e-22	89.0
MsG0380014546.01.T02	AT4G14540	46.429	84	45	0	20	103	26	109	4.05e-22	88.2
MsG0380014546.01.T02	AT2G38880	37.864	103	63	1	2	103	7	109	4.23e-22	86.7
MsG0380014546.01.T02	AT5G47640	46.429	84	45	0	20	103	32	115	4.66e-21	86.3
MsG0380014546.01.T02	AT2G37060	41.935	93	53	1	12	103	26	118	5.50e-21	85.5
MsG0380014546.01.T02	AT2G37060	41.935	93	53	1	12	103	26	118	5.50e-21	85.5
MsG0380014546.01.T02	AT2G37060	41.935	93	53	1	12	103	26	118	5.50e-21	85.5
MsG0380014546.01.T02	AT2G38880	37.864	103	63	1	2	103	7	109	6.58e-21	84.7
MsG0380014546.01.T02	AT2G38880	37.864	103	63	1	2	103	7	109	6.58e-21	84.7
MsG0380014546.01.T02	AT2G38880	37.864	103	63	1	2	103	7	109	6.58e-21	84.7
MsG0380014546.01.T02	AT2G13570	46.429	84	45	0	20	103	41	124	3.86e-20	84.3
MsG0380014546.01.T02	AT1G09030	40.476	84	50	0	20	103	8	91	8.89e-20	81.6
MsG0380014546.01.T02	AT1G21970	35.507	138	73	2	10	131	54	191	1.86e-19	83.2
MsG0380014546.01.T02	AT3G53340	39.785	93	55	1	12	103	25	117	4.16e-19	80.9
MsG0380014546.01.T02	AT3G53340	39.785	93	55	1	12	103	25	117	4.16e-19	80.9
MsG0380014546.01.T02	AT2G47810	40.476	84	50	0	20	103	56	139	4.41e-19	80.5
MsG0380014546.01.T02	AT3G53340	39.785	93	55	1	12	103	25	117	6.90e-19	79.0
MsG0380014546.01.T02	AT5G47670	38.298	94	57	1	11	103	24	117	1.19e-18	80.1
MsG0380014546.01.T02	AT5G47670	38.298	94	57	1	11	103	53	146	2.09e-18	80.1
MsG0380014546.01.T02	AT5G47670	38.298	94	57	1	11	103	53	146	2.09e-18	80.1
MsG0380014546.01.T02	AT3G53340	39.785	93	55	1	12	103	25	117	2.68e-18	78.2
MsG0380014546.01.T02	AT3G53340	39.785	93	55	1	12	103	25	117	2.68e-18	78.2

Find 47 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGGAATGAATTGGATAATT+TGG	0.149282	3:+56354821	MsG0380014546.01.T01:CDS
CAGGGAATGCAGGAAAATTT+TGG	0.155460	3:+56354533	MsG0380014546.01.T01:intron
CTCAAACTCATTTATTGATT+TGG	0.201982	3:+56354773	MsG0380014546.01.T01:CDS
ATTAAGGGTCATAAAGTCTA+TGG	0.242488	3:+56354569	MsG0380014546.01.T01:intron
TTTCGTTATAGTAATGAAAT+TGG	0.264459	3:+56354636	MsG0380014546.01.T01:intron
CCACCGCCACCCATTGCAAT+TGG	0.296308	3:+56354732	MsG0380014546.01.T01:CDS
CTTGGATTCATTATACTTGT+TGG	0.316399	3:-56354321	None:intergenic
GGCACAAGGGCATCATTTCT+TGG	0.320738	3:-56354339	None:intergenic
GGGTGGCGGTGGTGGTAAGG+TGG	0.341905	3:-56354721	None:intergenic
TAGACTTTATGACCCTTAAT+AGG	0.346002	3:-56354566	None:intergenic
TGGAATGAATTGGATAATTT+GGG	0.348468	3:+56354822	MsG0380014546.01.T01:CDS
GATGATAAAATGAATGATTA+AGG	0.370091	3:+56354924	MsG0380014546.01.T01:CDS
TTTGTCTCGAATTGCTAGTT+GGG	0.382494	3:-56354361	None:intergenic
GAATGAGGTTTGGAATGAAT+TGG	0.382994	3:+56354812	MsG0380014546.01.T01:CDS
TGGGTGCAGATGACCTATTA+AGG	0.383571	3:+56354553	MsG0380014546.01.T01:intron
CCACCCATTGCAATTGGGTC+AGG	0.408457	3:+56354738	MsG0380014546.01.T01:CDS
TACATATCTTATAAACTTTG+AGG	0.420260	3:-56354484	None:intergenic
GGGTGCAGATGACCTATTAA+GGG	0.425849	3:+56354554	MsG0380014546.01.T01:intron
TTCCCTGTGACAACGTTCAA+TGG	0.434461	3:-56354517	None:intergenic
ATTGGATATTTGAAACTTGT+TGG	0.437848	3:+56354654	MsG0380014546.01.T01:intron
GGTGGCGGTGGTGGTAAGGT+GGG	0.449680	3:-56354720	None:intergenic
CACCGCCACCCATTGCAATT+GGG	0.468956	3:+56354733	MsG0380014546.01.T01:CDS
CCAATTGCAATGGGTGGCGG+TGG	0.473098	3:-56354732	None:intergenic
CAGTGAGCATGAATGAGGTT+TGG	0.473614	3:+56354802	MsG0380014546.01.T01:CDS
AATGGGTGGCGGTGGTGGTA+AGG	0.484109	3:-56354724	None:intergenic
ATTGCAATGGGTGGCGGTGG+TGG	0.491572	3:-56354729	None:intergenic
GAATTGGATAATTTGGGTGC+TGG	0.493441	3:+56354828	MsG0380014546.01.T01:CDS
AAGCCATTGAACGTTGTCAC+AGG	0.498720	3:+56354514	MsG0380014546.01.T01:intron
TGACGATATCTTTCAAGAGA+TGG	0.505210	3:+56354902	MsG0380014546.01.T01:CDS
AACGTTGTCACAGGGAATGC+AGG	0.505564	3:+56354523	MsG0380014546.01.T01:intron
GACCCAATTGCAATGGGTGG+CGG	0.506575	3:-56354735	None:intergenic
ATCTGAAAAGCTGGTAAGGT+GGG	0.524201	3:-56354690	None:intergenic
CTCTCGTGGAGCAAAGGAGA+TGG	0.534626	3:+56354449	MsG0380014546.01.T01:intron
AAGCCTGACCCAATTGCAAT+GGG	0.536639	3:-56354741	None:intergenic
AAAGCTGGTAAGGTGGGCTT+AGG	0.541538	3:-56354684	None:intergenic
GAATTGCTAGTTGGGGCACA+AGG	0.564590	3:-56354353	None:intergenic
TTGTCTCGAATTGCTAGTTG+GGG	0.571725	3:-56354360	None:intergenic
TAAGCCTGACCCAATTGCAA+TGG	0.575839	3:-56354742	None:intergenic
TTAAGGGTCATAAAGTCTAT+GGG	0.580731	3:+56354570	MsG0380014546.01.T01:intron
AGCGATCTGAAAAGCTGGTA+AGG	0.583971	3:-56354694	None:intergenic
GGAGAAGCGATCTGAAAAGC+TGG	0.592152	3:-56354699	None:intergenic
AAATATTCCCTCGTTAACAA+TGG	0.602859	3:+56354010	MsG0380014546.01.T01:CDS
AGCCATTGAACGTTGTCACA+GGG	0.613629	3:+56354515	MsG0380014546.01.T01:intron
GATCTGAAAAGCTGGTAAGG+TGG	0.629020	3:-56354691	None:intergenic
CCTGACCCAATTGCAATGGG+TGG	0.641990	3:-56354738	None:intergenic
TGAAGCAGTGAGCATGAATG+AGG	0.652274	3:+56354797	MsG0380014546.01.T01:CDS
AATTGCTAGTTGGGGCACAA+GGG	0.680281	3:-56354352	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAATAATGAAAGTATTTATT+AGG	+	Chr3:56354243-56354262	MsG0380014546.01.T01:intron	10.0%
!!	TAAAATAACAATTTGATCTA+TGG	-	Chr3:56354154-56354173	None:intergenic	15.0%
!!	TACATATCTTATAAACTTTG+AGG	-	Chr3:56354487-56354506	None:intergenic	20.0%
!!	TTTCGTTATAGTAATGAAAT+TGG	+	Chr3:56354636-56354655	MsG0380014546.01.T01:intron	20.0%
!	TGGAATGAATTGGATAATTT+GGG	+	Chr3:56354822-56354841	MsG0380014546.01.T01:CDS	25.0%
!	TTGGAATGAATTGGATAATT+TGG	+	Chr3:56354821-56354840	MsG0380014546.01.T01:CDS	25.0%
!!	AGTTATTCTGATTTTGTGTT+TGG	-	Chr3:56354043-56354062	None:intergenic	25.0%
!!	ATTGGATATTTGAAACTTGT+TGG	+	Chr3:56354654-56354673	MsG0380014546.01.T01:intron	25.0%
!!	CTCAAACTCATTTATTGATT+TGG	+	Chr3:56354773-56354792	MsG0380014546.01.T01:CDS	25.0%
!!!	ATTGTTGCCACATTTTTTAT+TGG	-	Chr3:56354396-56354415	None:intergenic	25.0%
	AAATATTCCCTCGTTAACAA+TGG	+	Chr3:56354010-56354029	MsG0380014546.01.T01:CDS	30.0%
	AAGTATTTATTAGGATGTGC+AGG	+	Chr3:56354252-56354271	MsG0380014546.01.T01:intron	30.0%
	ATTAAGGGTCATAAAGTCTA+TGG	+	Chr3:56354569-56354588	MsG0380014546.01.T01:intron	30.0%
	CTTGGATTCATTATACTTGT+TGG	-	Chr3:56354324-56354343	None:intergenic	30.0%
	TACCTTGCCAATAAAAAATG+TGG	+	Chr3:56354386-56354405	MsG0380014546.01.T01:intron	30.0%
	TAGACTTTATGACCCTTAAT+AGG	-	Chr3:56354569-56354588	None:intergenic	30.0%
	TCATCTATGTTGCATCAAAT+TGG	+	Chr3:56354276-56354295	MsG0380014546.01.T01:intron	30.0%
	TGTCTCTAGATTTCAATACT+AGG	+	Chr3:56354095-56354114	MsG0380014546.01.T01:intron	30.0%
	TTAAGGGTCATAAAGTCTAT+GGG	+	Chr3:56354570-56354589	MsG0380014546.01.T01:intron	30.0%
!!	ATTTTGACCATTGTTAACGA+GGG	-	Chr3:56354020-56354039	None:intergenic	30.0%
	TGACGATATCTTTCAAGAGA+TGG	+	Chr3:56354902-56354921	MsG0380014546.01.T01:CDS	35.0%
	TTTGTCTCGAATTGCTAGTT+GGG	-	Chr3:56354364-56354383	None:intergenic	35.0%
!	AGGGAATGCAGGAAAATTTT+GGG	+	Chr3:56354534-56354553	MsG0380014546.01.T01:intron	35.0%
!	GAATGAGGTTTGGAATGAAT+TGG	+	Chr3:56354812-56354831	MsG0380014546.01.T01:CDS	35.0%
!	TTTTGTCTCGAATTGCTAGT+TGG	-	Chr3:56354365-56354384	None:intergenic	35.0%
!!	GATTTTGACCATTGTTAACG+AGG	-	Chr3:56354021-56354040	None:intergenic	35.0%
!!	TACTAGGTTGATAGATGAGT+TGG	+	Chr3:56354111-56354130	MsG0380014546.01.T01:intron	35.0%
!!!	ACAACGTTCAATGGCTTTTT+TGG	-	Chr3:56354511-56354530	None:intergenic	35.0%
!!!	TGCCACATTTTTTATTGGCA+AGG	-	Chr3:56354391-56354410	None:intergenic	35.0%
	AAAAATCTCTCGTGGAGCAA+AGG	+	Chr3:56354443-56354462	MsG0380014546.01.T01:intron	40.0%
	ATCTGAAAAGCTGGTAAGGT+GGG	-	Chr3:56354693-56354712	None:intergenic	40.0%
	CCAAACACAAAAATCTCTCG+TGG	+	Chr3:56354435-56354454	MsG0380014546.01.T01:intron	40.0%
	GAATTGGATAATTTGGGTGC+TGG	+	Chr3:56354828-56354847	MsG0380014546.01.T01:CDS	40.0%
	TTGTCTCGAATTGCTAGTTG+GGG	-	Chr3:56354363-56354382	None:intergenic	40.0%
!	CAGGGAATGCAGGAAAATTT+TGG	+	Chr3:56354533-56354552	MsG0380014546.01.T01:intron	40.0%
!	CCACGAGAGATTTTTGTGTT+TGG	-	Chr3:56354438-56354457	None:intergenic	40.0%
!	CGAGAGATTTTTGTGTTTGG+TGG	-	Chr3:56354435-56354454	None:intergenic	40.0%
	AAGCCATTGAACGTTGTCAC+AGG	+	Chr3:56354514-56354533	MsG0380014546.01.T01:intron	45.0%
	AAGCCTGACCCAATTGCAAT+GGG	-	Chr3:56354744-56354763	None:intergenic	45.0%
	AATTGCTAGTTGGGGCACAA+GGG	-	Chr3:56354355-56354374	None:intergenic	45.0%
	AGCCATTGAACGTTGTCACA+GGG	+	Chr3:56354515-56354534	MsG0380014546.01.T01:intron	45.0%
	AGCGATCTGAAAAGCTGGTA+AGG	-	Chr3:56354697-56354716	None:intergenic	45.0%
	CAGTGAGCATGAATGAGGTT+TGG	+	Chr3:56354802-56354821	MsG0380014546.01.T01:CDS	45.0%
	GATCTGAAAAGCTGGTAAGG+TGG	-	Chr3:56354694-56354713	None:intergenic	45.0%
	GGGTGCAGATGACCTATTAA+GGG	+	Chr3:56354554-56354573	MsG0380014546.01.T01:intron	45.0%
	TAAGCCTGACCCAATTGCAA+TGG	-	Chr3:56354745-56354764	None:intergenic	45.0%
	TGAAGCAGTGAGCATGAATG+AGG	+	Chr3:56354797-56354816	MsG0380014546.01.T01:CDS	45.0%
	TGGGTGCAGATGACCTATTA+AGG	+	Chr3:56354553-56354572	MsG0380014546.01.T01:intron	45.0%
	TTCCCTGTGACAACGTTCAA+TGG	-	Chr3:56354520-56354539	None:intergenic	45.0%
	AACGTTGTCACAGGGAATGC+AGG	+	Chr3:56354523-56354542	MsG0380014546.01.T01:intron	50.0%
	GAATTGCTAGTTGGGGCACA+AGG	-	Chr3:56354356-56354375	None:intergenic	50.0%
	GGAGAAGCGATCTGAAAAGC+TGG	-	Chr3:56354702-56354721	None:intergenic	50.0%
	TGGAGCTCTTGTATCTGCAG+AGG	-	Chr3:56354304-56354323	None:intergenic	50.0%
!	AAAGCTGGTAAGGTGGGCTT+AGG	-	Chr3:56354687-56354706	None:intergenic	50.0%
!!	GGCACAAGGGCATCATTTCT+TGG	-	Chr3:56354342-56354361	None:intergenic	50.0%
	CACCGCCACCCATTGCAATT+GGG	+	Chr3:56354733-56354752	MsG0380014546.01.T01:CDS	55.0%
	CCACCCATTGCAATTGGGTC+AGG	+	Chr3:56354738-56354757	MsG0380014546.01.T01:CDS	55.0%
	CCTGACCCAATTGCAATGGG+TGG	-	Chr3:56354741-56354760	None:intergenic	55.0%
	CTCTCGTGGAGCAAAGGAGA+TGG	+	Chr3:56354449-56354468	MsG0380014546.01.T01:intron	55.0%
	GACCCAATTGCAATGGGTGG+CGG	-	Chr3:56354738-56354757	None:intergenic	55.0%
	CCACCGCCACCCATTGCAAT+TGG	+	Chr3:56354732-56354751	MsG0380014546.01.T01:CDS	60.0%
!	AATGGGTGGCGGTGGTGGTA+AGG	-	Chr3:56354727-56354746	None:intergenic	60.0%
!	ATTGCAATGGGTGGCGGTGG+TGG	-	Chr3:56354732-56354751	None:intergenic	60.0%
!	CCAATTGCAATGGGTGGCGG+TGG	-	Chr3:56354735-56354754	None:intergenic	60.0%
!	GGTGGCGGTGGTGGTAAGGT+GGG	-	Chr3:56354723-56354742	None:intergenic	65.0%
!	GGGTGGCGGTGGTGGTAAGG+TGG	-	Chr3:56354724-56354743	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	56354001	56354944	56354001	ID=MsG0380014546.01;Name=MsG0380014546.01
Chr3	mRNA	56354001	56354944	56354001	ID=MsG0380014546.01.T01;Parent=MsG0380014546.01;Name=MsG0380014546.01.T01;_AED=0.26;_eAED=0.26;_QI=0\|0\|0\|1\|0\|0\|3\|0\|122
Chr3	exon	56354001	56354055	56354001	ID=MsG0380014546.01.T01:exon:18500;Parent=MsG0380014546.01.T01
Chr3	exon	56354310	56354389	56354310	ID=MsG0380014546.01.T01:exon:18501;Parent=MsG0380014546.01.T01
Chr3	exon	56354711	56354944	56354711	ID=MsG0380014546.01.T01:exon:18502;Parent=MsG0380014546.01.T01
Chr3	CDS	56354001	56354055	56354001	ID=MsG0380014546.01.T01:cds;Parent=MsG0380014546.01.T01
Chr3	CDS	56354310	56354389	56354310	ID=MsG0380014546.01.T01:cds;Parent=MsG0380014546.01.T01
Chr3	CDS	56354711	56354944	56354711	ID=MsG0380014546.01.T01:cds;Parent=MsG0380014546.01.T01
Chr3	mRNA	56354333	56354944	56354333	ID=MsG0380014546.01.T02;Parent=MsG0380014546.01;Name=MsG0380014546.01.T02;_AED=0.11;_eAED=0.11;_QI=0\|0\|0\|1\|0\|0\|2\|0\|193
Chr3	exon	56354333	56354694	56354333	ID=MsG0380014546.01.T02:exon:18503;Parent=MsG0380014546.01.T02
Chr3	exon	56354725	56354944	56354725	ID=MsG0380014546.01.T02:exon:18504;Parent=MsG0380014546.01.T02
Chr3	CDS	56354333	56354694	56354333	ID=MsG0380014546.01.T02:cds;Parent=MsG0380014546.01.T02
Chr3	CDS	56354725	56354944	56354725	ID=MsG0380014546.01.T02:cds;Parent=MsG0380014546.01.T02
Chr3	mRNA	56354417	56354944	56354417	ID=MsG0380014546.01.T03;Parent=MsG0380014546.01;Name=MsG0380014546.01.T03;_AED=0.15;_eAED=0.15;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|175
Chr3	exon	56354417	56354944	56354417	ID=MsG0380014546.01.T03:exon:18505;Parent=MsG0380014546.01.T03
Chr3	CDS	56354417	56354944	56354417	ID=MsG0380014546.01.T03:cds;Parent=MsG0380014546.01.T03

Gene Sequence

>MsG0380014546.01.T03

ATGAGAAAAGTTCTTCCACCAAACACAAAAATCTCTCGTGGAGCAAAGGAGATGGTTGAACAAATCACCTCAAAGTTTATAAGATATGTAACCAAAAAAGCCATTGAACGTTGTCACAGGGAATGCAGGAAAATTTTGGGTGCAGATGACCTATTAAGGGTCATAAAGTCTATGGGTTATAATAATTATTATGAGTATCTCAATTTTTATCTCAAATGCTTTCGTTATAGTAATGAAATTGGATATTTGAAACTTGTTGGTGAGCTTCCTAAGCCCACCTTACCAGCTTTTCAGATCGCTTCTCCCACCTTACCACCACCGCCACCCATTGCAATTGGGTCAGGCTTATCTTTGAACTCAAACTCATTTATTGATTTGGATGAAGCAGTGAGCATGAATGAGGTTTGGAATGAATTGGATAATTTGGGTGCTGGTTCTCTAGAGACTGCTTTAACAAACTTTGATCATTCAATTCAATTCAACTTTGACGATATCTTTCAAGAGATGGATGATAAAATGAATGATTAA

>MsG0380014546.01.T01

ATGGAGAATAAATATTCCCTCGTTAACAATGGTCAAAATCCAAACACAAAATCAGATACAAGAGCTCCAACAAGTATAATGAATCCAAGAAATGATGCCCTTGTGCCCCAACTAGCAATTCGAGACAAAAATACCATCGCTTCTCCCACCTTACCACCACCGCCACCCATTGCAATTGGGTCAGGCTTATCTTTGAACTCAAACTCATTTATTGATTTGGATGAAGCAGTGAGCATGAATGAGGTTTGGAATGAATTGGATAATTTGGGTGCTGGTTCTCTAGAGACTGCTTTAACAAACTTTGATCATTCAATTCAATTCAACTTTGACGATATCTTTCAAGAGATGGATGATAAAATGAATGATTAA

>MsG0380014546.01.T02

ATGAATCCAAGAAATGATGCCCTTGTGCCCCAACTAGCAATTCGAGACAAAAATACCTTGCCAATAAAAAATGTGGCAACAATCATGAGAAAAGTTCTTCCACCAAACACAAAAATCTCTCGTGGAGCAAAGGAGATGGTTGAACAAATCACCTCAAAGTTTATAAGATATGTAACCAAAAAAGCCATTGAACGTTGTCACAGGGAATGCAGGAAAATTTTGGGTGCAGATGACCTATTAAGGGTCATAAAGTCTATGGGTTATAATAATTATTATGAGTATCTCAATTTTTATCTCAAATGCTTTCGTTATAGTAATGAAATTGGATATTTGAAACTTGTTGGTGAGCTTCCTAAGCCCACCTTACCACCACCGCCACCCATTGCAATTGGGTCAGGCTTATCTTTGAACTCAAACTCATTTATTGATTTGGATGAAGCAGTGAGCATGAATGAGGTTTGGAATGAATTGGATAATTTGGGTGCTGGTTCTCTAGAGACTGCTTTAACAAACTTTGATCATTCAATTCAATTCAACTTTGACGATATCTTTCAAGAGATGGATGATAAAATGAATGATTAA

Protein sequence

>MsG0380014546.01.T03

MRKVLPPNTKISRGAKEMVEQITSKFIRYVTKKAIERCHRECRKILGADDLLRVIKSMGYNNYYEYLNFYLKCFRYSNEIGYLKLVGELPKPTLPAFQIASPTLPPPPPIAIGSGLSLNSNSFIDLDEAVSMNEVWNELDNLGAGSLETALTNFDHSIQFNFDDIFQEMDDKMND*

>MsG0380014546.01.T01

MENKYSLVNNGQNPNTKSDTRAPTSIMNPRNDALVPQLAIRDKNTIASPTLPPPPPIAIGSGLSLNSNSFIDLDEAVSMNEVWNELDNLGAGSLETALTNFDHSIQFNFDDIFQEMDDKMND*

>MsG0380014546.01.T02

MNPRNDALVPQLAIRDKNTLPIKNVATIMRKVLPPNTKISRGAKEMVEQITSKFIRYVTKKAIERCHRECRKILGADDLLRVIKSMGYNNYYEYLNFYLKCFRYSNEIGYLKLVGELPKPTLPPPPPIAIGSGLSLNSNSFIDLDEAVSMNEVWNELDNLGAGSLETALTNFDHSIQFNFDDIFQEMDDKMND*