Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014922.01.T02 | XP_003599289.1 | 83.66 | 153 | 25 | 0 | 1 | 153 | 31 | 183 | 5.81E-78 | 239 |
| MsG0380014922.01.T01 | XP_003599289.1 | 82.514 | 183 | 32 | 0 | 1 | 183 | 1 | 183 | 2.71E-97 | 289 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014922.01.T01 | Q9FJK3 | 38.994 | 159 | 92 | 2 | 1 | 157 | 1 | 156 | 3.39E-26 | 104 |
| MsG0380014922.01.T02 | Q9FJK3 | 35.156 | 128 | 78 | 2 | 2 | 127 | 32 | 156 | 5.61E-19 | 84.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014922.01.T01 | G7J0M2 | 82.514 | 183 | 32 | 0 | 1 | 183 | 1 | 183 | 1.29e-97 | 289 |
| MsG0380014922.01.T02 | G7J0M2 | 83.660 | 153 | 25 | 0 | 1 | 153 | 31 | 183 | 2.77e-78 | 239 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0380014922.01.T01 | TF | MADS-M-type |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014922.01.T02 | MTR_3g031240 | 83.660 | 153 | 25 | 0 | 1 | 153 | 31 | 183 | 7.04e-82 | 239 |
| MsG0380014922.01.T02 | MTR_3g031100 | 48.148 | 135 | 70 | 0 | 3 | 137 | 33 | 167 | 3.26e-39 | 133 |
| MsG0380014922.01.T02 | MTR_3g466980 | 47.581 | 124 | 64 | 1 | 2 | 125 | 32 | 154 | 1.29e-34 | 120 |
| MsG0380014922.01.T02 | MTR_4g063790 | 42.105 | 133 | 77 | 0 | 2 | 134 | 32 | 164 | 1.47e-31 | 111 |
| MsG0380014922.01.T02 | MTR_3g465410 | 42.105 | 133 | 77 | 0 | 2 | 134 | 4 | 136 | 9.28e-31 | 108 |
| MsG0380014922.01.T02 | MTR_3g065100 | 38.889 | 126 | 76 | 1 | 2 | 127 | 32 | 156 | 3.66e-29 | 107 |
| MsG0380014922.01.T02 | MTR_2g016210 | 40.299 | 134 | 80 | 0 | 2 | 135 | 32 | 165 | 1.24e-28 | 105 |
| MsG0380014922.01.T02 | MTR_3g467080 | 41.860 | 129 | 75 | 0 | 2 | 130 | 32 | 160 | 2.35e-28 | 102 |
| MsG0380014922.01.T02 | MTR_3g466830 | 42.105 | 133 | 70 | 3 | 2 | 131 | 31 | 159 | 2.76e-26 | 97.4 |
| MsG0380014922.01.T02 | MTR_3g466930 | 40.310 | 129 | 74 | 2 | 1 | 128 | 1 | 127 | 2.92e-26 | 96.7 |
| MsG0380014922.01.T02 | MTR_3g466900 | 41.085 | 129 | 73 | 2 | 1 | 128 | 1 | 127 | 3.12e-26 | 96.7 |
| MsG0380014922.01.T02 | MTR_4g032620 | 36.090 | 133 | 85 | 0 | 2 | 134 | 32 | 164 | 4.72e-26 | 99.0 |
| MsG0380014922.01.T02 | MTR_4g031910 | 35.433 | 127 | 82 | 0 | 2 | 128 | 32 | 158 | 4.76e-23 | 90.9 |
| MsG0380014922.01.T02 | MTR_4g032290 | 35.115 | 131 | 85 | 0 | 2 | 132 | 32 | 162 | 8.96e-23 | 90.5 |
| MsG0380014922.01.T02 | MTR_3g466890 | 38.760 | 129 | 76 | 2 | 1 | 128 | 30 | 156 | 6.30e-22 | 86.3 |
| MsG0380014922.01.T02 | MTR_5g075380 | 35.772 | 123 | 78 | 1 | 2 | 124 | 32 | 153 | 8.36e-22 | 86.3 |
| MsG0380014922.01.T02 | MTR_4g032260 | 32.258 | 124 | 84 | 0 | 2 | 125 | 32 | 155 | 6.81e-21 | 83.6 |
| MsG0380014922.01.T02 | MTR_8g036130 | 36.090 | 133 | 83 | 1 | 2 | 132 | 32 | 164 | 1.39e-20 | 85.1 |
| MsG0380014922.01.T02 | MTR_2g035610 | 40.964 | 83 | 49 | 0 | 2 | 84 | 32 | 114 | 1.56e-19 | 79.3 |
| MsG0380014922.01.T02 | MTR_2g035580 | 38.889 | 126 | 72 | 2 | 2 | 125 | 32 | 154 | 6.92e-19 | 78.6 |
| MsG0380014922.01.T02 | MTR_1g090710 | 35.433 | 127 | 81 | 1 | 2 | 128 | 32 | 157 | 7.10e-18 | 75.9 |
| MsG0380014922.01.T02 | MTR_1g077300 | 35.772 | 123 | 75 | 2 | 2 | 124 | 32 | 150 | 1.04e-16 | 72.8 |
| MsG0380014922.01.T02 | MTR_1g084950 | 34.921 | 126 | 80 | 2 | 2 | 127 | 32 | 155 | 1.50e-15 | 69.7 |
| MsG0380014922.01.T02 | MTR_1g077320 | 33.333 | 123 | 80 | 2 | 2 | 124 | 32 | 152 | 1.27e-14 | 67.4 |
| MsG0380014922.01.T02 | MTR_5g047580 | 34.109 | 129 | 77 | 3 | 2 | 127 | 32 | 155 | 2.35e-14 | 66.6 |
| MsG0380014922.01.T02 | MTR_1g090783 | 36.434 | 129 | 76 | 4 | 2 | 128 | 31 | 155 | 5.04e-14 | 65.9 |
| MsG0380014922.01.T02 | MTR_1g077390 | 32.520 | 123 | 81 | 2 | 2 | 124 | 32 | 152 | 7.21e-14 | 65.5 |
| MsG0380014922.01.T02 | MTR_7g011950 | 32.520 | 123 | 81 | 2 | 2 | 124 | 32 | 152 | 3.76e-13 | 63.5 |
| MsG0380014922.01.T02 | MTR_7g055800 | 35.366 | 82 | 38 | 1 | 3 | 84 | 20 | 86 | 1.17e-12 | 60.5 |
| MsG0380014922.01.T02 | MTR_1g090697 | 29.921 | 127 | 87 | 2 | 2 | 128 | 32 | 156 | 1.04e-11 | 59.7 |
| MsG0380014922.01.T02 | MTR_4g028720 | 30.894 | 123 | 83 | 2 | 2 | 124 | 32 | 152 | 1.59e-11 | 59.3 |
| MsG0380014922.01.T02 | MTR_4g019670 | 31.818 | 88 | 60 | 0 | 2 | 89 | 35 | 122 | 3.47e-11 | 60.5 |
| MsG0380014922.01.T01 | MTR_3g031240 | 82.514 | 183 | 32 | 0 | 1 | 183 | 1 | 183 | 3.28e-101 | 289 |
| MsG0380014922.01.T01 | MTR_3g031100 | 49.102 | 167 | 85 | 0 | 1 | 167 | 1 | 167 | 1.27e-52 | 168 |
| MsG0380014922.01.T01 | MTR_3g466980 | 49.032 | 155 | 78 | 1 | 1 | 155 | 1 | 154 | 1.75e-48 | 157 |
| MsG0380014922.01.T01 | MTR_4g063790 | 45.122 | 164 | 90 | 0 | 1 | 164 | 1 | 164 | 1.24e-45 | 148 |
| MsG0380014922.01.T01 | MTR_3g467080 | 45.000 | 160 | 88 | 0 | 1 | 160 | 1 | 160 | 2.15e-42 | 139 |
| MsG0380014922.01.T01 | MTR_2g016210 | 42.424 | 165 | 95 | 0 | 1 | 165 | 1 | 165 | 3.53e-41 | 139 |
| MsG0380014922.01.T01 | MTR_4g032620 | 38.415 | 164 | 101 | 0 | 1 | 164 | 1 | 164 | 7.87e-38 | 130 |
| MsG0380014922.01.T01 | MTR_3g065100 | 41.401 | 157 | 91 | 1 | 1 | 157 | 1 | 156 | 8.79e-37 | 127 |
| MsG0380014922.01.T01 | MTR_3g466830 | 41.975 | 162 | 87 | 3 | 3 | 161 | 2 | 159 | 1.71e-36 | 124 |
| MsG0380014922.01.T01 | MTR_8g036130 | 39.024 | 164 | 98 | 1 | 1 | 162 | 1 | 164 | 2.26e-33 | 119 |
| MsG0380014922.01.T01 | MTR_3g466890 | 40.127 | 157 | 91 | 2 | 3 | 158 | 2 | 156 | 1.52e-31 | 112 |
| MsG0380014922.01.T01 | MTR_5g075380 | 36.364 | 154 | 97 | 1 | 1 | 154 | 1 | 153 | 2.11e-31 | 111 |
| MsG0380014922.01.T01 | MTR_3g465410 | 41.912 | 136 | 79 | 0 | 29 | 164 | 1 | 136 | 4.07e-31 | 110 |
| MsG0380014922.01.T01 | MTR_4g031910 | 39.241 | 158 | 96 | 0 | 1 | 158 | 1 | 158 | 9.17e-31 | 112 |
| MsG0380014922.01.T01 | MTR_1g090710 | 37.975 | 158 | 97 | 1 | 1 | 158 | 1 | 157 | 7.52e-29 | 105 |
| MsG0380014922.01.T01 | MTR_2g035580 | 38.217 | 157 | 92 | 2 | 1 | 155 | 1 | 154 | 1.74e-27 | 101 |
| MsG0380014922.01.T01 | MTR_2g035610 | 43.860 | 114 | 64 | 0 | 1 | 114 | 1 | 114 | 2.69e-26 | 97.4 |
| MsG0380014922.01.T01 | MTR_3g466930 | 40.310 | 129 | 74 | 2 | 31 | 158 | 1 | 127 | 6.02e-26 | 97.1 |
| MsG0380014922.01.T01 | MTR_4g032290 | 36.420 | 162 | 103 | 0 | 1 | 162 | 1 | 162 | 6.86e-26 | 99.4 |
| MsG0380014922.01.T01 | MTR_3g466900 | 41.085 | 129 | 73 | 2 | 31 | 158 | 1 | 127 | 8.32e-26 | 96.7 |
| MsG0380014922.01.T01 | MTR_5g047580 | 36.250 | 160 | 94 | 3 | 1 | 157 | 1 | 155 | 1.50e-25 | 96.7 |
| MsG0380014922.01.T01 | MTR_1g077390 | 35.065 | 154 | 98 | 2 | 1 | 154 | 1 | 152 | 2.96e-25 | 95.9 |
| MsG0380014922.01.T01 | MTR_4g032260 | 34.194 | 155 | 102 | 0 | 1 | 155 | 1 | 155 | 3.66e-24 | 93.2 |
| MsG0380014922.01.T01 | MTR_1g090783 | 37.342 | 158 | 93 | 4 | 3 | 158 | 2 | 155 | 5.18e-24 | 92.8 |
| MsG0380014922.01.T01 | MTR_1g090697 | 33.544 | 158 | 103 | 2 | 1 | 158 | 1 | 156 | 2.33e-23 | 90.9 |
| MsG0380014922.01.T01 | MTR_1g077300 | 37.662 | 154 | 92 | 2 | 1 | 154 | 1 | 150 | 1.07e-22 | 89.4 |
| MsG0380014922.01.T01 | MTR_1g084950 | 36.306 | 157 | 98 | 2 | 1 | 157 | 1 | 155 | 1.95e-21 | 85.9 |
| MsG0380014922.01.T01 | MTR_1g077320 | 33.766 | 154 | 100 | 2 | 1 | 154 | 1 | 152 | 3.86e-20 | 82.8 |
| MsG0380014922.01.T01 | MTR_7g011950 | 33.766 | 154 | 100 | 2 | 1 | 154 | 1 | 152 | 5.31e-20 | 82.4 |
| MsG0380014922.01.T01 | MTR_4g028720 | 33.766 | 154 | 100 | 2 | 1 | 154 | 1 | 152 | 2.31e-18 | 78.2 |
| MsG0380014922.01.T01 | MTR_4g028800 | 43.836 | 73 | 39 | 1 | 1 | 73 | 1 | 71 | 5.87e-18 | 74.7 |
| MsG0380014922.01.T01 | MTR_7g106510 | 28.205 | 117 | 84 | 0 | 3 | 119 | 6 | 122 | 5.03e-16 | 75.5 |
| MsG0380014922.01.T01 | MTR_4g019670 | 29.915 | 117 | 82 | 0 | 3 | 119 | 6 | 122 | 1.70e-15 | 73.9 |
| MsG0380014922.01.T01 | MTR_6g018920 | 32.743 | 113 | 69 | 3 | 1 | 107 | 1 | 112 | 1.83e-15 | 73.2 |
| MsG0380014922.01.T01 | MTR_1g114730 | 31.897 | 116 | 72 | 3 | 1 | 110 | 1 | 115 | 1.06e-14 | 71.2 |
| MsG0380014922.01.T01 | MTR_6g005450 | 31.858 | 113 | 70 | 3 | 1 | 107 | 1 | 112 | 3.18e-14 | 69.7 |
| MsG0380014922.01.T01 | MTR_7g055800 | 31.858 | 113 | 49 | 2 | 1 | 113 | 1 | 85 | 1.61e-13 | 63.5 |
| MsG0380014922.01.T01 | MTR_4g107170 | 27.660 | 188 | 115 | 7 | 1 | 176 | 1 | 179 | 3.97e-13 | 65.9 |
| MsG0380014922.01.T01 | MTR_6g005440 | 28.571 | 126 | 83 | 3 | 1 | 120 | 1 | 125 | 4.94e-12 | 63.5 |
| MsG0380014922.01.T01 | MTR_5g047560 | 33.058 | 121 | 64 | 2 | 1 | 121 | 1 | 104 | 4.98e-12 | 60.8 |
| MsG0380014922.01.T01 | MTR_7g055790 | 33.945 | 109 | 54 | 1 | 3 | 111 | 61 | 151 | 4.99e-12 | 61.2 |
| MsG0380014922.01.T01 | MTR_3g093900 | 24.204 | 157 | 110 | 2 | 3 | 155 | 6 | 157 | 1.93e-11 | 62.0 |
| MsG0380014922.01.T01 | MTR_7g075850 | 35.398 | 113 | 63 | 2 | 1 | 104 | 1 | 112 | 3.82e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014922.01.T02 | AT5G48670 | 35.156 | 128 | 78 | 2 | 2 | 127 | 32 | 156 | 5.72e-20 | 84.3 |
| MsG0380014922.01.T02 | AT5G27810 | 43.023 | 86 | 49 | 0 | 2 | 87 | 4 | 89 | 3.32e-19 | 78.2 |
| MsG0380014922.01.T02 | AT1G65330 | 29.104 | 134 | 92 | 2 | 3 | 135 | 32 | 163 | 2.20e-15 | 71.6 |
| MsG0380014922.01.T02 | AT3G05860 | 31.967 | 122 | 78 | 2 | 2 | 121 | 32 | 150 | 2.72e-15 | 70.1 |
| MsG0380014922.01.T02 | AT1G65300 | 37.209 | 86 | 54 | 0 | 2 | 87 | 31 | 116 | 2.98e-15 | 71.2 |
| MsG0380014922.01.T02 | AT3G05860 | 31.707 | 123 | 79 | 2 | 2 | 122 | 32 | 151 | 3.99e-15 | 70.5 |
| MsG0380014922.01.T02 | AT3G05860 | 31.967 | 122 | 78 | 2 | 2 | 121 | 32 | 150 | 5.30e-15 | 70.1 |
| MsG0380014922.01.T02 | AT5G26650 | 37.209 | 86 | 54 | 0 | 2 | 87 | 30 | 115 | 8.35e-15 | 70.5 |
| MsG0380014922.01.T02 | AT5G26630 | 32.308 | 130 | 75 | 3 | 4 | 127 | 34 | 156 | 1.55e-14 | 68.6 |
| MsG0380014922.01.T02 | AT5G27960 | 34.884 | 86 | 56 | 0 | 2 | 87 | 30 | 115 | 1.14e-13 | 67.0 |
| MsG0380014922.01.T02 | AT2G28700 | 31.959 | 97 | 66 | 0 | 2 | 98 | 32 | 128 | 4.60e-13 | 65.5 |
| MsG0380014922.01.T02 | AT5G06500 | 30.973 | 113 | 61 | 3 | 2 | 112 | 32 | 129 | 4.03e-12 | 62.4 |
| MsG0380014922.01.T02 | AT1G31640 | 32.558 | 86 | 57 | 1 | 2 | 87 | 31 | 115 | 8.09e-11 | 59.3 |
| MsG0380014922.01.T01 | AT5G48670 | 38.994 | 159 | 92 | 2 | 1 | 157 | 1 | 156 | 3.45e-27 | 104 |
| MsG0380014922.01.T01 | AT3G05860 | 35.948 | 153 | 93 | 2 | 1 | 151 | 1 | 150 | 4.09e-27 | 102 |
| MsG0380014922.01.T01 | AT3G05860 | 35.714 | 154 | 94 | 2 | 1 | 152 | 1 | 151 | 1.13e-26 | 102 |
| MsG0380014922.01.T01 | AT3G05860 | 35.714 | 154 | 94 | 2 | 1 | 152 | 1 | 151 | 1.43e-26 | 102 |
| MsG0380014922.01.T01 | AT1G65330 | 33.333 | 156 | 101 | 2 | 3 | 157 | 2 | 155 | 1.61e-24 | 97.1 |
| MsG0380014922.01.T01 | AT1G22590 | 31.737 | 167 | 98 | 2 | 1 | 161 | 1 | 157 | 7.87e-24 | 92.4 |
| MsG0380014922.01.T01 | AT2G28700 | 35.156 | 128 | 83 | 0 | 1 | 128 | 1 | 128 | 1.11e-23 | 95.9 |
| MsG0380014922.01.T01 | AT1G65300 | 38.261 | 115 | 71 | 0 | 3 | 117 | 2 | 116 | 1.65e-23 | 94.4 |
| MsG0380014922.01.T01 | AT5G26650 | 37.719 | 114 | 71 | 0 | 4 | 117 | 2 | 115 | 4.12e-22 | 92.0 |
| MsG0380014922.01.T01 | AT5G27960 | 36.842 | 114 | 72 | 0 | 4 | 117 | 2 | 115 | 6.01e-22 | 90.9 |
| MsG0380014922.01.T01 | AT5G26630 | 35.583 | 163 | 92 | 3 | 1 | 157 | 1 | 156 | 3.50e-20 | 84.3 |
| MsG0380014922.01.T01 | AT5G06500 | 31.944 | 144 | 81 | 3 | 1 | 142 | 1 | 129 | 4.41e-20 | 84.7 |
| MsG0380014922.01.T01 | AT5G27810 | 42.697 | 89 | 51 | 0 | 29 | 117 | 1 | 89 | 1.63e-19 | 80.1 |
| MsG0380014922.01.T01 | AT1G31630 | 34.783 | 115 | 74 | 1 | 3 | 117 | 2 | 115 | 2.39e-19 | 84.0 |
| MsG0380014922.01.T01 | AT1G31640 | 34.783 | 115 | 74 | 1 | 3 | 117 | 2 | 115 | 6.29e-17 | 78.2 |
| MsG0380014922.01.T01 | AT2G40210 | 37.000 | 100 | 63 | 0 | 1 | 100 | 1 | 100 | 3.42e-16 | 75.5 |
| MsG0380014922.01.T01 | AT5G26580 | 32.479 | 117 | 65 | 2 | 1 | 117 | 1 | 103 | 8.60e-15 | 71.6 |
Find 27 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTACCAAAAGTTGGATTTAA+TGG | 0.132212 | 3:-62539614 | MsG0380014922.01.T01:CDS |
| TTCCGTAACTTCATTAGAAT+TGG | 0.312999 | 3:+62539663 | None:intergenic |
| AATCCATTAAATCCAACTTT+TGG | 0.339004 | 3:+62539611 | None:intergenic |
| GAGGTTTGGCCTTCTCCAAC+TGG | 0.351491 | 3:-62539898 | MsG0380014922.01.T01:CDS |
| GAAGCTAGATAAAATTCATT+TGG | 0.361908 | 3:+62539541 | None:intergenic |
| GACCAATTCTAATGAAGTTA+CGG | 0.448005 | 3:-62539665 | MsG0380014922.01.T01:CDS |
| ACCATCATGAAGCTGAGGTT+TGG | 0.453221 | 3:-62539912 | MsG0380014922.01.T01:CDS |
| AACGAATTGAGCATCCTTTG+TGG | 0.463741 | 3:-62539967 | MsG0380014922.01.T01:CDS |
| CAATTGAATCCTCTTGAGCT+TGG | 0.465024 | 3:+62539587 | None:intergenic |
| ATTGATTAATGAGAAATTGA+AGG | 0.477565 | 3:-62539641 | MsG0380014922.01.T01:CDS |
| GGAAAACAAGAAGAAAGAAA+TGG | 0.479640 | 3:-62539746 | MsG0380014922.01.T01:CDS |
| GAAGGAAGTCTACCAAAAGT+TGG | 0.485133 | 3:-62539623 | MsG0380014922.01.T01:CDS |
| GCCAAACCTCAGCTTCATGA+TGG | 0.527299 | 3:+62539911 | None:intergenic |
| ATCATCAAAATTAAATTCAG+TGG | 0.561119 | 3:+62539696 | None:intergenic |
| CACTACTATGGGCAGAAGGA+AGG | 0.561138 | 3:-62541508 | None:intergenic |
| AGATCAGTATCACAAACTAA+AGG | 0.570022 | 3:-62539770 | MsG0380014922.01.T01:CDS |
| GAGTAGTAAGTGTGTTGTCA+AGG | 0.577943 | 3:-62539876 | MsG0380014922.01.T01:CDS |
| CTTACTACTCCAGTTGGAGA+AGG | 0.592421 | 3:+62539889 | None:intergenic |
| TCAGTATCACAAACTAAAGG+TGG | 0.593746 | 3:-62539767 | MsG0380014922.01.T01:CDS |
| AACACACTTACTACTCCAGT+TGG | 0.596457 | 3:+62539883 | None:intergenic |
| TACTATGGGCAGAAGGAAGG+TGG | 0.600474 | 3:-62541505 | None:intergenic |
| TCATCACTACTATGGGCAGA+AGG | 0.612227 | 3:-62541512 | None:intergenic |
| AATGGATTTCCAAGCTCAAG+AGG | 0.613698 | 3:-62539596 | MsG0380014922.01.T01:CDS |
| TGAAAACCATCATGAAGCTG+AGG | 0.619064 | 3:-62539917 | MsG0380014922.01.T01:CDS |
| TAGTCAACAACTCAAAGCGG+AGG | 0.677810 | 3:-62541470 | MsG0380014922.01.T01:CDS |
| CACAAGCATTGATACCACAA+AGG | 0.692894 | 3:+62539953 | None:intergenic |
| ACATAGTCAACAACTCAAAG+CGG | 0.715101 | 3:-62541473 | MsG0380014922.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATAATAATTTTTTTTATA+AGG | - | Chr3:62540794-62540813 | MsG0380014922.01.T01:intron | 0.0% |
| !! | CAATTATGTATAAAAATAAA+TGG | + | Chr3:62539808-62539827 | None:intergenic | 10.0% |
| !!! | ACTTTTTATTTTATTTCTAA+TGG | + | Chr3:62539869-62539888 | None:intergenic | 10.0% |
| !!! | CTTATTTTACTTTTTTTTTA+AGG | + | Chr3:62540600-62540619 | None:intergenic | 10.0% |
| !!! | TTATTTTATTTATAGATTTG+AGG | - | Chr3:62540489-62540508 | MsG0380014922.01.T01:intron | 10.0% |
| !!! | ATTCATGTATATTTTTCAAT+AGG | - | Chr3:62541029-62541048 | MsG0380014922.01.T01:intron | 15.0% |
| !!! | CGTTCTTTATTTTATAAAAA+AGG | - | Chr3:62540642-62540661 | MsG0380014922.01.T01:intron | 15.0% |
| !!! | TAAGTCTTTTTTTTTTTTGA+AGG | - | Chr3:62540336-62540355 | MsG0380014922.01.T01:intron | 15.0% |
| !!! | TTATAAAAAAGGTAATTTGT+TGG | - | Chr3:62540653-62540672 | MsG0380014922.01.T01:intron | 15.0% |
| !!! | TTCATGTATATTTTTCAATA+GGG | - | Chr3:62541030-62541049 | MsG0380014922.01.T01:intron | 15.0% |
| !!! | TTTTTCCTTTTTTTTTTAAC+AGG | - | Chr3:62540077-62540096 | MsG0380014922.01.T01:intron | 15.0% |
| !! | ACATCAAAATTGAATATTGT+TGG | - | Chr3:62540181-62540200 | MsG0380014922.01.T01:intron | 20.0% |
| !! | ATCATCAAAATTAAATTCAG+TGG | + | Chr3:62541342-62541361 | None:intergenic | 20.0% |
| !! | ATTGATTAATGAGAAATTGA+AGG | - | Chr3:62541394-62541413 | MsG0380014922.01.T01:intron | 20.0% |
| !! | CTTATCCTGTTAAAAAAAAA+AGG | + | Chr3:62540085-62540104 | None:intergenic | 20.0% |
| !! | TACACAAAATCAAATATGAT+TGG | + | Chr3:62540467-62540486 | None:intergenic | 20.0% |
| !! | TCCAAATCAAAATTTCAAAT+TGG | - | Chr3:62540142-62540161 | MsG0380014922.01.T01:intron | 20.0% |
| !! | TGATTATAATCTTTGACAAT+CGG | - | Chr3:62539704-62539723 | MsG0380014922.01.T01:CDS | 20.0% |
| !!! | AACGTTTTTTTAAATTGTTG+AGG | + | Chr3:62540560-62540579 | None:intergenic | 20.0% |
| !!! | ACCAATTTGAAATTTTGATT+TGG | + | Chr3:62540146-62540165 | None:intergenic | 20.0% |
| !!! | ACCATTTTTATTACAGAATT+AGG | - | Chr3:62539959-62539978 | MsG0380014922.01.T01:CDS | 20.0% |
| !!! | CCATTTTTATTACAGAATTA+GGG | - | Chr3:62539960-62539979 | MsG0380014922.01.T01:CDS | 20.0% |
| !!! | CTTTTGGTTTGATAAATTTT+CGG | - | Chr3:62540982-62541001 | MsG0380014922.01.T01:intron | 20.0% |
| !!! | GAATATTGTTGGATATTTTT+GGG | - | Chr3:62540192-62540211 | MsG0380014922.01.T01:intron | 20.0% |
| !!! | TGAATATTGTTGGATATTTT+TGG | - | Chr3:62540191-62540210 | MsG0380014922.01.T01:intron | 20.0% |
| !!! | TTTTTTTATGTCATTTGTTC+TGG | + | Chr3:62540820-62540839 | None:intergenic | 20.0% |
| !!! | TTTTTTTCATCTGTGAAAAA+TGG | + | Chr3:62540015-62540034 | None:intergenic | 20.0% |
| ! | AAATCGTGTAAAATCGTTAA+TGG | - | Chr3:62540032-62540051 | MsG0380014922.01.T01:intron | 25.0% |
| ! | ACATTCAGAACAAGAAAAAA+TGG | - | Chr3:62539591-62539610 | MsG0380014922.01.T01:CDS | 25.0% |
| ! | ATCCAGTATGAGATTAATAT+TGG | - | Chr3:62539766-62539785 | MsG0380014922.01.T01:CDS | 25.0% |
| ! | CAAAGATTATAATCATAGTG+TGG | + | Chr3:62539699-62539718 | None:intergenic | 25.0% |
| ! | CAAATGACATAAAAAAAGAG+TGG | - | Chr3:62540823-62540842 | MsG0380014922.01.T01:intron | 25.0% |
| ! | CCCTAATTCTGTAATAAAAA+TGG | + | Chr3:62539963-62539982 | None:intergenic | 25.0% |
| ! | GAAGCTAGATAAAATTCATT+TGG | + | Chr3:62541497-62541516 | None:intergenic | 25.0% |
| ! | TAAACAATTAGGGTAAGTTA+CGG | + | Chr3:62540685-62540704 | None:intergenic | 25.0% |
| ! | TCCAGTATGAGATTAATATT+GGG | - | Chr3:62539767-62539786 | MsG0380014922.01.T01:CDS | 25.0% |
| !! | AATCCATTAAATCCAACTTT+TGG | + | Chr3:62541427-62541446 | None:intergenic | 25.0% |
| !!! | GTTTTTTTAAATTGTTGAGG+TGG | + | Chr3:62540557-62540576 | None:intergenic | 25.0% |
| !!! | TAAAAAAGGTAATTTGTTGG+TGG | - | Chr3:62540656-62540675 | MsG0380014922.01.T01:intron | 25.0% |
| ACCCAATATTAATCTCATAC+TGG | + | Chr3:62539771-62539790 | None:intergenic | 30.0% | |
| AGATCAGTATCACAAACTAA+AGG | - | Chr3:62541265-62541284 | MsG0380014922.01.T01:intron | 30.0% | |
| ATAAAGAACGAAAATCGTCA+TGG | + | Chr3:62540632-62540651 | None:intergenic | 30.0% | |
| ATCTGACAAAACATTAGAGT+TGG | + | Chr3:62540868-62540887 | None:intergenic | 30.0% | |
| CTACCAAAAGTTGGATTTAA+TGG | - | Chr3:62541421-62541440 | MsG0380014922.01.T01:intron | 30.0% | |
| GACCAATTCTAATGAAGTTA+CGG | - | Chr3:62541370-62541389 | MsG0380014922.01.T01:intron | 30.0% | |
| GGAAAACAAGAAGAAAGAAA+TGG | - | Chr3:62541289-62541308 | MsG0380014922.01.T01:intron | 30.0% | |
| TCTGACAAAACATTAGAGTT+GGG | + | Chr3:62540867-62540886 | None:intergenic | 30.0% | |
| TTCCGTAACTTCATTAGAAT+TGG | + | Chr3:62541375-62541394 | None:intergenic | 30.0% | |
| ! | TACAGAATTAGGGTAAGTTT+TGG | - | Chr3:62539970-62539989 | MsG0380014922.01.T01:CDS | 30.0% |
| !!! | CCTTGTTCTTTTTTCATGTT+CGG | + | Chr3:62541198-62541217 | None:intergenic | 30.0% |
| ACATAGTCAACAACTCAAAG+CGG | - | Chr3:62539562-62539581 | MsG0380014922.01.T01:CDS | 35.0% | |
| AGTTGGGTACCAATATAGTT+GGG | + | Chr3:62540851-62540870 | None:intergenic | 35.0% | |
| CCGAACATGAAAAAAGAACA+AGG | - | Chr3:62541195-62541214 | MsG0380014922.01.T01:intron | 35.0% | |
| TCAGTATCACAAACTAAAGG+TGG | - | Chr3:62541268-62541287 | MsG0380014922.01.T01:intron | 35.0% | |
| TGAAAAAAGAACAAGGATGC+TGG | - | Chr3:62541202-62541221 | MsG0380014922.01.T01:intron | 35.0% | |
| TTAACAGGATAAGCTGATGT+TGG | - | Chr3:62540092-62540111 | MsG0380014922.01.T01:intron | 35.0% | |
| AACACACTTACTACTCCAGT+TGG | + | Chr3:62541155-62541174 | None:intergenic | 40.0% | |
| AACGAATTGAGCATCCTTTG+TGG | - | Chr3:62541068-62541087 | MsG0380014922.01.T01:intron | 40.0% | |
| CAATTGAATCCTCTTGAGCT+TGG | + | Chr3:62541451-62541470 | None:intergenic | 40.0% | |
| CTAACTCGTAAAGATGCCTT+GGG | + | Chr3:62539737-62539756 | None:intergenic | 40.0% | |
| GAAGGAAGTCTACCAAAAGT+TGG | - | Chr3:62541412-62541431 | MsG0380014922.01.T01:intron | 40.0% | |
| GAGTAGTAAGTGTGTTGTCA+AGG | - | Chr3:62541159-62541178 | MsG0380014922.01.T01:intron | 40.0% | |
| GAGTTGGGTACCAATATAGT+TGG | + | Chr3:62540852-62540871 | None:intergenic | 40.0% | |
| TAACTCGTAAAGATGCCTTG+GGG | + | Chr3:62539736-62539755 | None:intergenic | 40.0% | |
| TGAAAAATGGCCCAAGTCTT+TGG | + | Chr3:62540002-62540021 | None:intergenic | 40.0% | |
| TGAAAACCATCATGAAGCTG+AGG | - | Chr3:62541118-62541137 | MsG0380014922.01.T01:intron | 40.0% | |
| ! | AATGGATTTCCAAGCTCAAG+AGG | - | Chr3:62541439-62541458 | MsG0380014922.01.T01:intron | 40.0% |
| !! | CACAAGCATTGATACCACAA+AGG | + | Chr3:62541085-62541104 | None:intergenic | 40.0% |
| ACCATCATGAAGCTGAGGTT+TGG | - | Chr3:62541123-62541142 | MsG0380014922.01.T01:intron | 45.0% | |
| AGAGTGGCTCCCAACTATAT+TGG | - | Chr3:62540839-62540858 | MsG0380014922.01.T01:intron | 45.0% | |
| CCTAACTCGTAAAGATGCCT+TGG | + | Chr3:62539738-62539757 | None:intergenic | 45.0% | |
| CTTACTACTCCAGTTGGAGA+AGG | + | Chr3:62541149-62541168 | None:intergenic | 45.0% | |
| TAGTCAACAACTCAAAGCGG+AGG | - | Chr3:62539565-62539584 | MsG0380014922.01.T01:CDS | 45.0% | |
| TTGGCTGTCACCAAAGACTT+GGG | - | Chr3:62539989-62540008 | MsG0380014922.01.T01:intron | 45.0% | |
| TTTGGCTGTCACCAAAGACT+TGG | - | Chr3:62539988-62540007 | MsG0380014922.01.T01:intron | 45.0% | |
| ! | CCAAGGCATCTTTACGAGTT+AGG | - | Chr3:62539735-62539754 | MsG0380014922.01.T01:CDS | 45.0% |
| !!! | AAAAAAAAAAAAAATTCTTT+TGG | - | Chr3:62540966-62540985 | MsG0380014922.01.T01:intron | 5.0% |
| !!! | AAAAAGTATTTAAAAAATTA+GGG | - | Chr3:62539882-62539901 | MsG0380014922.01.T01:CDS | 5.0% |
| !!! | ATATTTTATATTAAACAATT+AGG | + | Chr3:62540696-62540715 | None:intergenic | 5.0% |
| !!! | TAAAAAGTATTTAAAAAATT+AGG | - | Chr3:62539881-62539900 | MsG0380014922.01.T01:CDS | 5.0% |
| !!! | TATTTTATATTAAACAATTA+GGG | + | Chr3:62540695-62540714 | None:intergenic | 5.0% |
| !!! | TTATTTTACTTTTTTTTTAA+GGG | + | Chr3:62540599-62540618 | None:intergenic | 5.0% |
| GCCAAACCTCAGCTTCATGA+TGG | + | Chr3:62541127-62541146 | None:intergenic | 50.0% | |
| ! | GAGGTTTGGCCTTCTCCAAC+TGG | - | Chr3:62541137-62541156 | MsG0380014922.01.T01:intron | 55.0% |
| GACAATCGGTGTGTGCCCCA+AGG | - | Chr3:62539718-62539737 | MsG0380014922.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 62539534 | 62541523 | 62539534 | ID=MsG0380014922.01;Name=MsG0380014922.01 |
| Chr3 | mRNA | 62539534 | 62541523 | 62539534 | ID=MsG0380014922.01.T01;Parent=MsG0380014922.01;Name=MsG0380014922.01.T01;_AED=0.00;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|183 |
| Chr3 | exon | 62539534 | 62540006 | 62539534 | ID=MsG0380014922.01.T01:exon:8497;Parent=MsG0380014922.01.T01 |
| Chr3 | exon | 62541445 | 62541523 | 62541445 | ID=MsG0380014922.01.T01:exon:8496;Parent=MsG0380014922.01.T01 |
| Chr3 | CDS | 62541445 | 62541523 | 62541445 | ID=MsG0380014922.01.T01:cds;Parent=MsG0380014922.01.T01 |
| Chr3 | CDS | 62539534 | 62540006 | 62539534 | ID=MsG0380014922.01.T01:cds;Parent=MsG0380014922.01.T01 |
| Chr3 | mRNA | 62539534 | 62539995 | 62539534 | ID=MsG0380014922.01.T02;Parent=MsG0380014922.01;Name=MsG0380014922.01.T02;_AED=0.09;_eAED=0.09;_QI=0|-1|0|1|-1|0|1|0|153 |
| Chr3 | exon | 62539534 | 62539995 | 62539534 | ID=MsG0380014922.01.T02:exon:8498;Parent=MsG0380014922.01.T02 |
| Chr3 | CDS | 62539534 | 62539995 | 62539534 | ID=MsG0380014922.01.T02:cds;Parent=MsG0380014922.01.T02 |
| Gene Sequence |
| Protein sequence |
