Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014957.01.T01 | AES61431.2 | 88.489 | 278 | 17 | 1 | 1 | 263 | 1 | 278 | 7.95E-179 | 503 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014957.01.T01 | Q9SZL8 | 25.214 | 234 | 148 | 7 | 35 | 263 | 72 | 283 | 3.66E-12 | 69.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014957.01.T01 | G7I8T1 | 88.489 | 278 | 17 | 1 | 1 | 263 | 1 | 278 | 3.80e-179 | 503 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0380014957.01.T01 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014957.01.T01 | MTR_1g083640 | 88.489 | 278 | 17 | 1 | 1 | 263 | 1 | 278 | 0.0 | 503 |
| MsG0380014957.01.T01 | MTR_0558s0020 | 91.255 | 263 | 23 | 0 | 1 | 263 | 1 | 263 | 0.0 | 502 |
| MsG0380014957.01.T01 | MTR_2g014100 | 85.714 | 245 | 27 | 1 | 1 | 237 | 1 | 245 | 9.37e-154 | 429 |
| MsG0380014957.01.T01 | MTR_8g446020 | 77.186 | 263 | 20 | 2 | 1 | 263 | 1 | 223 | 8.01e-141 | 396 |
| MsG0380014957.01.T01 | MTR_3g008240 | 87.685 | 203 | 25 | 0 | 1 | 203 | 1 | 203 | 8.58e-131 | 369 |
| MsG0380014957.01.T01 | MTR_6g090505 | 89.899 | 198 | 20 | 0 | 66 | 263 | 1 | 198 | 8.83e-131 | 375 |
| MsG0380014957.01.T01 | MTR_0337s0040 | 70.498 | 261 | 38 | 1 | 1 | 261 | 1 | 222 | 3.61e-128 | 363 |
| MsG0380014957.01.T01 | MTR_4g040430 | 87.685 | 203 | 20 | 2 | 1 | 203 | 1 | 198 | 1.24e-127 | 360 |
| MsG0380014957.01.T01 | MTR_5g071590 | 82.778 | 180 | 19 | 1 | 1 | 168 | 1 | 180 | 5.41e-107 | 308 |
| MsG0380014957.01.T01 | MTR_0019s0290 | 89.542 | 153 | 16 | 0 | 82 | 234 | 8 | 160 | 9.97e-100 | 288 |
| MsG0380014957.01.T01 | MTR_1g055240 | 90.909 | 143 | 13 | 0 | 91 | 233 | 2 | 144 | 1.46e-95 | 278 |
| MsG0380014957.01.T01 | MTR_1281s0010 | 92.661 | 109 | 8 | 0 | 20 | 128 | 1 | 109 | 4.77e-70 | 211 |
| MsG0380014957.01.T01 | MTR_4g008820 | 87.500 | 80 | 10 | 0 | 1 | 80 | 125 | 204 | 1.49e-38 | 143 |
| MsG0380014957.01.T01 | MTR_8g046060 | 81.818 | 88 | 8 | 2 | 1 | 80 | 91 | 178 | 2.60e-37 | 131 |
| MsG0380014957.01.T01 | MTR_6g051425 | 26.339 | 224 | 148 | 5 | 41 | 263 | 22 | 229 | 3.30e-16 | 77.8 |
| MsG0380014957.01.T01 | MTR_0402s0040 | 24.865 | 185 | 120 | 4 | 50 | 234 | 40 | 205 | 1.97e-13 | 70.1 |
| MsG0380014957.01.T01 | MTR_1g013060 | 28.636 | 220 | 136 | 9 | 41 | 257 | 29 | 230 | 7.69e-13 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014957.01.T01 | AT4G38180 | 25.214 | 234 | 148 | 7 | 35 | 263 | 72 | 283 | 3.73e-13 | 69.7 |
Find 40 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGTTATGGGTCAAGGATT+TGG | 0.277051 | 3:-63177770 | MsG0380014957.01.T01:CDS |
| TTTCAATCTATTAAAGAGAT+TGG | 0.308439 | 3:-63178226 | MsG0380014957.01.T01:CDS |
| TCTGATGTTGGCCTGTTGTT+AGG | 0.329765 | 3:+63178049 | None:intergenic |
| GCACGGAGAATCTTCCTTAT+CGG | 0.372197 | 3:-63177745 | MsG0380014957.01.T01:CDS |
| GAGAACGTCTTATGAAGTTA+TGG | 0.394356 | 3:-63177783 | MsG0380014957.01.T01:CDS |
| GCTCTAATAAATTCTAAAGT+TGG | 0.394745 | 3:-63177815 | MsG0380014957.01.T01:CDS |
| ATGGGTATAAGATATATAGA+TGG | 0.430188 | 3:-63177928 | MsG0380014957.01.T01:CDS |
| TTTAAGTCTGAAAACCGATA+AGG | 0.441874 | 3:+63177731 | None:intergenic |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | 3:-63177980 | MsG0380014957.01.T01:CDS |
| CGAAAAGTAAATGAAGTTCA+AGG | 0.448756 | 3:-63177845 | MsG0380014957.01.T01:CDS |
| TCTAATGACAATTCGTCAAA+AGG | 0.456711 | 3:-63177702 | MsG0380014957.01.T01:CDS |
| AGAACGTCTTATGAAGTTAT+GGG | 0.466325 | 3:-63177782 | MsG0380014957.01.T01:CDS |
| TCCATGATTCGTTATTGCTA+GGG | 0.479627 | 3:+63178260 | None:intergenic |
| GTTTGCTTAATCCTAACAAC+AGG | 0.484661 | 3:-63178060 | MsG0380014957.01.T01:CDS |
| CCCCTAGCAATAACGAATCA+TGG | 0.494071 | 3:-63178261 | MsG0380014957.01.T01:CDS |
| CGTTATGTTTGTAACAAACA+AGG | 0.494754 | 3:-63178097 | MsG0380014957.01.T01:CDS |
| CTCAGGTAACACAAATGCTC+TGG | 0.496027 | 3:+63177875 | None:intergenic |
| AGCTGGAATATCTTTGAAGC+TGG | 0.496270 | 3:-63177963 | MsG0380014957.01.T01:CDS |
| TTACAACCAACCCTTTCCTC+AGG | 0.505082 | 3:+63177986 | None:intergenic |
| TTCACAAACTCAATAACAAG+TGG | 0.508337 | 3:+63178022 | None:intergenic |
| TTCCATGATTCGTTATTGCT+AGG | 0.509536 | 3:+63178259 | None:intergenic |
| TAACAAACAAGGTTTCAAGA+AGG | 0.511682 | 3:-63178086 | MsG0380014957.01.T01:CDS |
| AAATGCTCTGGTTTATGTAA+TGG | 0.514358 | 3:+63177887 | None:intergenic |
| GGTAATTATGCATATAACAC+TGG | 0.523547 | 3:-63178193 | MsG0380014957.01.T01:CDS |
| TGGGTATAAGATATATAGAT+GGG | 0.523615 | 3:-63177927 | MsG0380014957.01.T01:CDS |
| ACAAAATAAGAAATCAGATA+AGG | 0.530342 | 3:-63178131 | MsG0380014957.01.T01:CDS |
| TGTCTTCCGCAGTATCAACT+TGG | 0.544233 | 3:+63177597 | None:intergenic |
| TCTTATGAAGTTATGGGTCA+AGG | 0.549558 | 3:-63177776 | MsG0380014957.01.T01:CDS |
| GTTATGGGTCAAGGATTTGG+AGG | 0.554514 | 3:-63177767 | MsG0380014957.01.T01:CDS |
| AACAAACAAGGTTTCAAGAA+GGG | 0.554561 | 3:-63178085 | MsG0380014957.01.T01:CDS |
| CGTCAAAAGGAGATGGTGGT+TGG | 0.556443 | 3:-63177689 | MsG0380014957.01.T01:CDS |
| GACAATTCGTCAAAAGGAGA+TGG | 0.566160 | 3:-63177696 | MsG0380014957.01.T01:CDS |
| TAAGATATATAGATGGGATG+TGG | 0.566406 | 3:-63177921 | MsG0380014957.01.T01:CDS |
| TGGATGTCATAGTAACAAGA+TGG | 0.572286 | 3:+63177617 | None:intergenic |
| CCATGATTCGTTATTGCTAG+GGG | 0.572877 | 3:+63178261 | None:intergenic |
| ATATCAACTTCATTTGTGAG+TGG | 0.587339 | 3:-63178340 | MsG0380014957.01.T01:CDS |
| ACAAGTGGATTGTCTGATGT+TGG | 0.615496 | 3:+63178037 | None:intergenic |
| GGGTCAAGGATTTGGAGGCA+CGG | 0.631027 | 3:-63177762 | MsG0380014957.01.T01:CDS |
| AATTCGTCAAAAGGAGATGG+TGG | 0.636402 | 3:-63177693 | MsG0380014957.01.T01:CDS |
| TGACATCCAAGTTGATACTG+CGG | 0.720725 | 3:-63177603 | MsG0380014957.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAATAAGAAATCAGATA+AGG | - | Chr3:63177780-63177799 | MsG0380014957.01.T01:CDS | 20.0% |
| !! | TTTCAATCTATTAAAGAGAT+TGG | - | Chr3:63177685-63177704 | MsG0380014957.01.T01:CDS | 20.0% |
| ! | ATGGGTATAAGATATATAGA+TGG | - | Chr3:63177983-63178002 | MsG0380014957.01.T01:CDS | 25.0% |
| ! | TGGGTATAAGATATATAGAT+GGG | - | Chr3:63177984-63178003 | MsG0380014957.01.T01:CDS | 25.0% |
| !! | AAAGAGATTGGAGATTTTTA+TGG | - | Chr3:63177697-63177716 | MsG0380014957.01.T01:CDS | 25.0% |
| !! | ATAACACTGGATTTTCTATT+AGG | - | Chr3:63177731-63177750 | MsG0380014957.01.T01:CDS | 25.0% |
| !!! | GCTCTAATAAATTCTAAAGT+TGG | - | Chr3:63178096-63178115 | MsG0380014957.01.T01:CDS | 25.0% |
| AACAAACAAGGTTTCAAGAA+GGG | - | Chr3:63177826-63177845 | MsG0380014957.01.T01:CDS | 30.0% | |
| AGAACGTCTTATGAAGTTAT+GGG | - | Chr3:63178129-63178148 | MsG0380014957.01.T01:CDS | 30.0% | |
| ATATCAACTTCATTTGTGAG+TGG | - | Chr3:63177571-63177590 | MsG0380014957.01.T01:CDS | 30.0% | |
| CGAAAAGTAAATGAAGTTCA+AGG | - | Chr3:63178066-63178085 | MsG0380014957.01.T01:CDS | 30.0% | |
| CGTTATGTTTGTAACAAACA+AGG | - | Chr3:63177814-63177833 | MsG0380014957.01.T01:CDS | 30.0% | |
| GGTAATTATGCATATAACAC+TGG | - | Chr3:63177718-63177737 | MsG0380014957.01.T01:CDS | 30.0% | |
| TAACAAACAAGGTTTCAAGA+AGG | - | Chr3:63177825-63177844 | MsG0380014957.01.T01:CDS | 30.0% | |
| TAAGATATATAGATGGGATG+TGG | - | Chr3:63177990-63178009 | MsG0380014957.01.T01:CDS | 30.0% | |
| TCTAATGACAATTCGTCAAA+AGG | - | Chr3:63178209-63178228 | MsG0380014957.01.T01:CDS | 30.0% | |
| TTCACAAACTCAATAACAAG+TGG | + | Chr3:63177892-63177911 | None:intergenic | 30.0% | |
| TTTAAGTCTGAAAACCGATA+AGG | + | Chr3:63178183-63178202 | None:intergenic | 30.0% | |
| ! | AAATGCTCTGGTTTATGTAA+TGG | + | Chr3:63178027-63178046 | None:intergenic | 30.0% |
| !! | TTACTTTTCGAAATGATCTC+AGG | + | Chr3:63178056-63178075 | None:intergenic | 30.0% |
| GAAAGAAAAACCTGAGGAAA+GGG | - | Chr3:63177915-63177934 | MsG0380014957.01.T01:CDS | 35.0% | |
| GAAAGTGAAAGAAAAACCTG+AGG | - | Chr3:63177909-63177928 | MsG0380014957.01.T01:CDS | 35.0% | |
| GAGAACGTCTTATGAAGTTA+TGG | - | Chr3:63178128-63178147 | MsG0380014957.01.T01:CDS | 35.0% | |
| GTTTGCTTAATCCTAACAAC+AGG | - | Chr3:63177851-63177870 | MsG0380014957.01.T01:CDS | 35.0% | |
| TCCATGATTCGTTATTGCTA+GGG | + | Chr3:63177654-63177673 | None:intergenic | 35.0% | |
| TCTTATGAAGTTATGGGTCA+AGG | - | Chr3:63178135-63178154 | MsG0380014957.01.T01:CDS | 35.0% | |
| TGAAAGAAAAACCTGAGGAA+AGG | - | Chr3:63177914-63177933 | MsG0380014957.01.T01:CDS | 35.0% | |
| TGGATGTCATAGTAACAAGA+TGG | + | Chr3:63178297-63178316 | None:intergenic | 35.0% | |
| TTCCATGATTCGTTATTGCT+AGG | + | Chr3:63177655-63177674 | None:intergenic | 35.0% | |
| !!! | AAGCTGGATGAAGTTTTGAA+TGG | - | Chr3:63177964-63177983 | MsG0380014957.01.T01:CDS | 35.0% |
| !!! | AGCTGGATGAAGTTTTGAAT+GGG | - | Chr3:63177965-63177984 | MsG0380014957.01.T01:CDS | 35.0% |
| AATTCGTCAAAAGGAGATGG+TGG | - | Chr3:63178218-63178237 | MsG0380014957.01.T01:CDS | 40.0% | |
| CCATGATTCGTTATTGCTAG+GGG | + | Chr3:63177653-63177672 | None:intergenic | 40.0% | |
| GAAGTTATGGGTCAAGGATT+TGG | - | Chr3:63178141-63178160 | MsG0380014957.01.T01:CDS | 40.0% | |
| GACAATTCGTCAAAAGGAGA+TGG | - | Chr3:63178215-63178234 | MsG0380014957.01.T01:CDS | 40.0% | |
| !! | ACAAGTGGATTGTCTGATGT+TGG | + | Chr3:63177877-63177896 | None:intergenic | 40.0% |
| !! | AGCTGGAATATCTTTGAAGC+TGG | - | Chr3:63177948-63177967 | MsG0380014957.01.T01:CDS | 40.0% |
| !! | TGACATCCAAGTTGATACTG+CGG | - | Chr3:63178308-63178327 | MsG0380014957.01.T01:CDS | 40.0% |
| CCCCTAGCAATAACGAATCA+TGG | - | Chr3:63177650-63177669 | MsG0380014957.01.T01:CDS | 45.0% | |
| CTCAGGTAACACAAATGCTC+TGG | + | Chr3:63178039-63178058 | None:intergenic | 45.0% | |
| GAAAAACCTGAGGAAAGGGT+TGG | - | Chr3:63177919-63177938 | MsG0380014957.01.T01:CDS | 45.0% | |
| GCACGGAGAATCTTCCTTAT+CGG | - | Chr3:63178166-63178185 | MsG0380014957.01.T01:CDS | 45.0% | |
| GTTATGGGTCAAGGATTTGG+AGG | - | Chr3:63178144-63178163 | MsG0380014957.01.T01:CDS | 45.0% | |
| TGTCTTCCGCAGTATCAACT+TGG | + | Chr3:63178317-63178336 | None:intergenic | 45.0% | |
| TTACAACCAACCCTTTCCTC+AGG | + | Chr3:63177928-63177947 | None:intergenic | 45.0% | |
| ! | TCTGATGTTGGCCTGTTGTT+AGG | + | Chr3:63177865-63177884 | None:intergenic | 45.0% |
| !! | GAAAGGGTTGGTTGTAAAGC+TGG | - | Chr3:63177931-63177950 | MsG0380014957.01.T01:CDS | 45.0% |
| ! | CGTCAAAAGGAGATGGTGGT+TGG | - | Chr3:63178222-63178241 | MsG0380014957.01.T01:CDS | 50.0% |
| GGGTCAAGGATTTGGAGGCA+CGG | - | Chr3:63178149-63178168 | MsG0380014957.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 63177562 | 63178371 | 63177562 | ID=MsG0380014957.01;Name=MsG0380014957.01 |
| Chr3 | mRNA | 63177562 | 63178371 | 63177562 | ID=MsG0380014957.01.T01;Parent=MsG0380014957.01;Name=MsG0380014957.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|269 |
| Chr3 | exon | 63177562 | 63178371 | 63177562 | ID=MsG0380014957.01.T01:exon:8691;Parent=MsG0380014957.01.T01 |
| Chr3 | CDS | 63177562 | 63178371 | 63177562 | ID=MsG0380014957.01.T01:cds;Parent=MsG0380014957.01.T01 |
| Gene Sequence |
| Protein sequence |
