Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014974.01.T01 | RHN67862.1 | 91.304 | 161 | 14 | 0 | 1 | 161 | 1 | 161 | 6.34E-103 | 302 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014974.01.T01 | Q9FJK3 | 43.396 | 159 | 90 | 0 | 2 | 160 | 3 | 161 | 1.05E-32 | 121 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014974.01.T01 | A0A396IQL1 | 91.304 | 161 | 14 | 0 | 1 | 161 | 1 | 161 | 3.03e-103 | 302 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0380014974.01.T01 | TF | MADS-M-type |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014974.01.T01 | MTR_3g466890 | 88.199 | 161 | 19 | 0 | 1 | 161 | 1 | 161 | 9.42e-102 | 289 |
| MsG0380014974.01.T01 | MTR_3g466830 | 78.750 | 160 | 34 | 0 | 1 | 160 | 1 | 160 | 1.38e-88 | 256 |
| MsG0380014974.01.T01 | MTR_3g466900 | 89.394 | 132 | 14 | 0 | 30 | 161 | 1 | 132 | 8.14e-85 | 245 |
| MsG0380014974.01.T01 | MTR_3g466930 | 84.091 | 132 | 21 | 0 | 30 | 161 | 1 | 132 | 4.87e-78 | 228 |
| MsG0380014974.01.T01 | MTR_3g065100 | 49.682 | 157 | 79 | 0 | 2 | 158 | 3 | 159 | 2.99e-45 | 149 |
| MsG0380014974.01.T01 | MTR_8g036130 | 46.250 | 160 | 83 | 2 | 2 | 158 | 3 | 162 | 8.47e-42 | 140 |
| MsG0380014974.01.T01 | MTR_4g031910 | 47.799 | 159 | 82 | 1 | 2 | 159 | 3 | 161 | 1.04e-41 | 139 |
| MsG0380014974.01.T01 | MTR_3g466980 | 41.875 | 160 | 93 | 0 | 2 | 161 | 3 | 162 | 3.79e-41 | 137 |
| MsG0380014974.01.T01 | MTR_4g032260 | 49.020 | 153 | 77 | 1 | 1 | 152 | 2 | 154 | 7.04e-38 | 127 |
| MsG0380014974.01.T01 | MTR_1g090697 | 44.516 | 155 | 85 | 1 | 2 | 156 | 3 | 156 | 1.84e-37 | 126 |
| MsG0380014974.01.T01 | MTR_4g032620 | 40.252 | 159 | 92 | 2 | 2 | 158 | 3 | 160 | 3.74e-37 | 127 |
| MsG0380014974.01.T01 | MTR_4g032290 | 46.250 | 160 | 85 | 1 | 1 | 159 | 2 | 161 | 2.94e-36 | 125 |
| MsG0380014974.01.T01 | MTR_3g031240 | 40.764 | 157 | 90 | 2 | 2 | 156 | 3 | 158 | 1.15e-35 | 122 |
| MsG0380014974.01.T01 | MTR_2g035610 | 49.167 | 120 | 61 | 0 | 2 | 121 | 3 | 122 | 7.67e-35 | 118 |
| MsG0380014974.01.T01 | MTR_5g075380 | 41.772 | 158 | 92 | 0 | 4 | 161 | 5 | 162 | 8.51e-35 | 119 |
| MsG0380014974.01.T01 | MTR_1g090783 | 42.038 | 157 | 90 | 1 | 1 | 157 | 1 | 156 | 9.73e-35 | 119 |
| MsG0380014974.01.T01 | MTR_3g031100 | 37.580 | 157 | 97 | 1 | 2 | 157 | 3 | 159 | 7.33e-32 | 114 |
| MsG0380014974.01.T01 | MTR_1g090710 | 42.308 | 156 | 88 | 2 | 2 | 156 | 3 | 157 | 3.81e-31 | 110 |
| MsG0380014974.01.T01 | MTR_5g047580 | 40.909 | 154 | 90 | 1 | 2 | 155 | 3 | 155 | 1.80e-30 | 108 |
| MsG0380014974.01.T01 | MTR_2g016210 | 41.781 | 146 | 84 | 1 | 4 | 148 | 5 | 150 | 1.45e-29 | 108 |
| MsG0380014974.01.T01 | MTR_4g028720 | 42.857 | 154 | 87 | 1 | 2 | 155 | 3 | 155 | 6.52e-28 | 102 |
| MsG0380014974.01.T01 | MTR_1g077390 | 40.132 | 152 | 90 | 1 | 1 | 152 | 2 | 152 | 6.89e-28 | 102 |
| MsG0380014974.01.T01 | MTR_3g467080 | 34.839 | 155 | 100 | 1 | 2 | 155 | 3 | 157 | 1.69e-27 | 100 |
| MsG0380014974.01.T01 | MTR_1g077320 | 42.857 | 154 | 87 | 1 | 2 | 155 | 3 | 155 | 6.44e-27 | 99.4 |
| MsG0380014974.01.T01 | MTR_7g011950 | 42.208 | 154 | 88 | 1 | 2 | 155 | 3 | 155 | 7.41e-27 | 99.4 |
| MsG0380014974.01.T01 | MTR_1g084950 | 40.260 | 154 | 91 | 1 | 2 | 155 | 3 | 155 | 2.03e-25 | 95.5 |
| MsG0380014974.01.T01 | MTR_4g063790 | 34.161 | 161 | 93 | 2 | 2 | 155 | 3 | 157 | 4.23e-24 | 92.4 |
| MsG0380014974.01.T01 | MTR_1g077300 | 39.216 | 153 | 88 | 3 | 1 | 152 | 2 | 150 | 1.18e-20 | 83.2 |
| MsG0380014974.01.T01 | MTR_4g028800 | 54.412 | 68 | 29 | 1 | 4 | 71 | 5 | 70 | 2.38e-20 | 80.1 |
| MsG0380014974.01.T01 | MTR_2g035580 | 35.811 | 148 | 95 | 0 | 5 | 152 | 6 | 153 | 8.18e-20 | 81.3 |
| MsG0380014974.01.T01 | MTR_7g106510 | 31.373 | 153 | 104 | 1 | 2 | 154 | 6 | 157 | 1.88e-17 | 78.6 |
| MsG0380014974.01.T01 | MTR_4g019670 | 30.719 | 153 | 105 | 1 | 2 | 154 | 6 | 157 | 2.12e-17 | 78.6 |
| MsG0380014974.01.T01 | MTR_3g093900 | 28.387 | 155 | 111 | 0 | 2 | 156 | 6 | 160 | 1.92e-16 | 75.9 |
| MsG0380014974.01.T01 | MTR_5g047560 | 40.909 | 110 | 48 | 3 | 2 | 111 | 3 | 95 | 2.44e-16 | 71.6 |
| MsG0380014974.01.T01 | MTR_3g465410 | 32.090 | 134 | 78 | 2 | 29 | 155 | 2 | 129 | 3.52e-16 | 71.2 |
| MsG0380014974.01.T01 | MTR_7g055800 | 33.333 | 117 | 50 | 2 | 2 | 118 | 3 | 91 | 2.08e-13 | 62.8 |
| MsG0380014974.01.T01 | MTR_7g055790 | 37.963 | 108 | 49 | 2 | 2 | 109 | 61 | 150 | 5.31e-11 | 57.8 |
| MsG0380014974.01.T01 | MTR_6g018920 | 35.135 | 111 | 63 | 5 | 2 | 105 | 3 | 111 | 6.27e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014974.01.T01 | AT5G48670 | 43.396 | 159 | 90 | 0 | 2 | 160 | 3 | 161 | 1.07e-33 | 121 |
| MsG0380014974.01.T01 | AT1G65300 | 37.500 | 152 | 95 | 0 | 1 | 152 | 1 | 152 | 2.71e-29 | 108 |
| MsG0380014974.01.T01 | AT1G65330 | 37.975 | 158 | 98 | 0 | 1 | 158 | 1 | 158 | 2.91e-28 | 106 |
| MsG0380014974.01.T01 | AT5G26630 | 37.580 | 157 | 98 | 0 | 2 | 158 | 3 | 159 | 5.28e-27 | 101 |
| MsG0380014974.01.T01 | AT3G05860 | 35.948 | 153 | 98 | 0 | 2 | 154 | 3 | 155 | 2.76e-24 | 94.0 |
| MsG0380014974.01.T01 | AT3G05860 | 35.948 | 153 | 98 | 0 | 2 | 154 | 3 | 155 | 7.32e-24 | 94.0 |
| MsG0380014974.01.T01 | AT3G05860 | 35.948 | 153 | 98 | 0 | 2 | 154 | 3 | 155 | 9.46e-24 | 94.0 |
| MsG0380014974.01.T01 | AT5G27960 | 31.613 | 155 | 106 | 0 | 4 | 158 | 3 | 157 | 7.07e-23 | 92.8 |
| MsG0380014974.01.T01 | AT5G26650 | 31.613 | 155 | 106 | 0 | 4 | 158 | 3 | 157 | 9.07e-23 | 92.8 |
| MsG0380014974.01.T01 | AT5G27810 | 43.478 | 92 | 52 | 0 | 29 | 120 | 2 | 93 | 1.71e-21 | 84.3 |
| MsG0380014974.01.T01 | AT1G31630 | 28.571 | 161 | 114 | 1 | 1 | 161 | 1 | 160 | 2.84e-18 | 80.5 |
| MsG0380014974.01.T01 | AT2G28700 | 30.968 | 155 | 101 | 2 | 2 | 153 | 3 | 154 | 2.01e-17 | 77.8 |
| MsG0380014974.01.T01 | AT5G26580 | 31.544 | 149 | 88 | 1 | 4 | 152 | 5 | 139 | 4.47e-17 | 77.0 |
| MsG0380014974.01.T01 | AT1G31640 | 29.139 | 151 | 106 | 1 | 1 | 151 | 1 | 150 | 4.91e-17 | 77.4 |
| MsG0380014974.01.T01 | AT1G22590 | 33.663 | 101 | 67 | 0 | 2 | 102 | 3 | 103 | 1.59e-14 | 67.4 |
| MsG0380014974.01.T01 | AT2G40210 | 36.364 | 99 | 63 | 0 | 2 | 100 | 3 | 101 | 4.51e-12 | 63.2 |
| MsG0380014974.01.T01 | AT5G06500 | 29.167 | 120 | 85 | 0 | 2 | 121 | 3 | 122 | 2.61e-11 | 60.1 |
Find 35 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAAAAGTTATTCAGAATT+CGG | 0.234784 | 3:-63464977 | MsG0380014974.01.T01:CDS |
| TGAGAAGTCAAAGGAGAAAT+TGG | 0.315486 | 3:-63464906 | MsG0380014974.01.T01:CDS |
| TCAAAAGTTATTCAGAATTC+GGG | 0.321565 | 3:-63464976 | MsG0380014974.01.T01:CDS |
| GAAGGTGAAACTTGCATTCA+TGG | 0.384908 | 3:-63465176 | MsG0380014974.01.T01:CDS |
| GGAAATTAATGACAAGCTTA+AGG | 0.410494 | 3:-63464768 | MsG0380014974.01.T01:CDS |
| TTTGCATCAAATAGCTCTCT+TGG | 0.411513 | 3:+63464933 | None:intergenic |
| AAAGGAGAAATTGGTGAAAG+TGG | 0.424135 | 3:-63464897 | MsG0380014974.01.T01:CDS |
| GGACTTCTCCATCTCCCAAT+TGG | 0.458797 | 3:+63464868 | None:intergenic |
| GAAAGTGGAGAAGGCCAATT+GGG | 0.464215 | 3:-63464882 | MsG0380014974.01.T01:CDS |
| TGAAAGTGGAGAAGGCCAAT+TGG | 0.469746 | 3:-63464883 | MsG0380014974.01.T01:CDS |
| TAATCCTTTGTACCTCCAAT+GGG | 0.474838 | 3:+63465008 | None:intergenic |
| AATGGGGAAGGCCATACCTC+TGG | 0.503415 | 3:+63465025 | None:intergenic |
| AGAGGTATGGCCTTCCCCAT+TGG | 0.513167 | 3:-63465023 | MsG0380014974.01.T01:CDS |
| ATAATCCTTTGTACCTCCAA+TGG | 0.513473 | 3:+63465007 | None:intergenic |
| AAGAGAGCTATTTGATGCAA+AGG | 0.518848 | 3:-63464931 | MsG0380014974.01.T01:CDS |
| GATGAACTCACTACCCTATG+TGG | 0.541478 | 3:-63465091 | MsG0380014974.01.T01:CDS |
| GCCTACATCAATCCCACATA+GGG | 0.544450 | 3:+63465078 | None:intergenic |
| ATTCAGAATTCGGGCAAGGA+AGG | 0.545789 | 3:-63464967 | MsG0380014974.01.T01:CDS |
| TTCATTCAGAGCCAGAGGTA+TGG | 0.547696 | 3:-63465036 | MsG0380014974.01.T01:CDS |
| ACCCTATGTGGGATTGATGT+AGG | 0.551331 | 3:-63465079 | MsG0380014974.01.T01:CDS |
| GGTATGGCCTTCCCCATTGG+AGG | 0.554434 | 3:-63465020 | MsG0380014974.01.T01:CDS |
| AAGACACTGTAACAAGATCA+AGG | 0.558997 | 3:+63464847 | None:intergenic |
| CTTCCCCATTGGAGGTACAA+AGG | 0.572974 | 3:-63465012 | MsG0380014974.01.T01:CDS |
| GGAGAAGGCCAATTGGGAGA+TGG | 0.573197 | 3:-63464876 | MsG0380014974.01.T01:CDS |
| AGTTATTCAGAATTCGGGCA+AGG | 0.591523 | 3:-63464971 | MsG0380014974.01.T01:CDS |
| CGCCTACATCAATCCCACAT+AGG | 0.593049 | 3:+63465077 | None:intergenic |
| GCAAAGGATTGAGAAGTCAA+AGG | 0.593345 | 3:-63464915 | MsG0380014974.01.T01:CDS |
| GAAATTGGTGAAAGTGGAGA+AGG | 0.595364 | 3:-63464891 | MsG0380014974.01.T01:CDS |
| TGGTTATCGAAATCTCTAAG+TGG | 0.599795 | 3:+63464706 | None:intergenic |
| ATGAACTCACTACCCTATGT+GGG | 0.601587 | 3:-63465090 | MsG0380014974.01.T01:CDS |
| CTTTGTACCTCCAATGGGGA+AGG | 0.604550 | 3:+63465013 | None:intergenic |
| CGAATTTCATTCAGAGCCAG+AGG | 0.606659 | 3:-63465041 | MsG0380014974.01.T01:CDS |
| TCATGGTCAATGATTCTGCA+AGG | 0.642899 | 3:-63465159 | MsG0380014974.01.T01:CDS |
| AATCCTTTGTACCTCCAATG+GGG | 0.720306 | 3:+63465009 | None:intergenic |
| TACATCGAGGATGAGAACGA+AGG | 0.737605 | 3:-63465194 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTCAAAAGTTATTCAGAATT+CGG | - | Chr3:63464928-63464947 | MsG0380014974.01.T01:CDS | 20.0% |
| ! | TCAAAAGTTATTCAGAATTC+GGG | - | Chr3:63464929-63464948 | MsG0380014974.01.T01:CDS | 25.0% |
| AAAAAAGAGTCTGAAGAAGA+TGG | - | Chr3:63464789-63464808 | MsG0380014974.01.T01:CDS | 30.0% | |
| GGAAATTAATGACAAGCTTA+AGG | - | Chr3:63465137-63465156 | MsG0380014974.01.T01:CDS | 30.0% | |
| !! | ATGTTTTGAATGATCTAGCA+TGG | - | Chr3:63465115-63465134 | MsG0380014974.01.T01:CDS | 30.0% |
| !! | TGTTTTGAATGATCTAGCAT+GGG | - | Chr3:63465116-63465135 | MsG0380014974.01.T01:CDS | 30.0% |
| AAAGGAGAAATTGGTGAAAG+TGG | - | Chr3:63465008-63465027 | MsG0380014974.01.T01:CDS | 35.0% | |
| AAGACACTGTAACAAGATCA+AGG | + | Chr3:63465061-63465080 | None:intergenic | 35.0% | |
| AAGAGAGCTATTTGATGCAA+AGG | - | Chr3:63464974-63464993 | MsG0380014974.01.T01:CDS | 35.0% | |
| ATAATCCTTTGTACCTCCAA+TGG | + | Chr3:63464901-63464920 | None:intergenic | 35.0% | |
| TAATCCTTTGTACCTCCAAT+GGG | + | Chr3:63464900-63464919 | None:intergenic | 35.0% | |
| TGAGAAGTCAAAGGAGAAAT+TGG | - | Chr3:63464999-63465018 | MsG0380014974.01.T01:CDS | 35.0% | |
| TTTGCATCAAATAGCTCTCT+TGG | + | Chr3:63464975-63464994 | None:intergenic | 35.0% | |
| AATCCTTTGTACCTCCAATG+GGG | + | Chr3:63464899-63464918 | None:intergenic | 40.0% | |
| AGTTATTCAGAATTCGGGCA+AGG | - | Chr3:63464934-63464953 | MsG0380014974.01.T01:CDS | 40.0% | |
| ATGAACTCACTACCCTATGT+GGG | - | Chr3:63464815-63464834 | MsG0380014974.01.T01:CDS | 40.0% | |
| GAAATTGGTGAAAGTGGAGA+AGG | - | Chr3:63465014-63465033 | MsG0380014974.01.T01:CDS | 40.0% | |
| GAAGGTGAAACTTGCATTCA+TGG | - | Chr3:63464729-63464748 | MsG0380014974.01.T01:CDS | 40.0% | |
| GCAAAGGATTGAGAAGTCAA+AGG | - | Chr3:63464990-63465009 | MsG0380014974.01.T01:CDS | 40.0% | |
| TCATGGTCAATGATTCTGCA+AGG | - | Chr3:63464746-63464765 | MsG0380014974.01.T01:CDS | 40.0% | |
| ACCCTATGTGGGATTGATGT+AGG | - | Chr3:63464826-63464845 | MsG0380014974.01.T01:CDS | 45.0% | |
| ATTCAGAATTCGGGCAAGGA+AGG | - | Chr3:63464938-63464957 | MsG0380014974.01.T01:CDS | 45.0% | |
| CGAATTTCATTCAGAGCCAG+AGG | - | Chr3:63464864-63464883 | MsG0380014974.01.T01:CDS | 45.0% | |
| GAAAGTGGAGAAGGCCAATT+GGG | - | Chr3:63465023-63465042 | MsG0380014974.01.T01:CDS | 45.0% | |
| GATGAACTCACTACCCTATG+TGG | - | Chr3:63464814-63464833 | MsG0380014974.01.T01:CDS | 45.0% | |
| GCCTACATCAATCCCACATA+GGG | + | Chr3:63464830-63464849 | None:intergenic | 45.0% | |
| TGAAAGTGGAGAAGGCCAAT+TGG | - | Chr3:63465022-63465041 | MsG0380014974.01.T01:CDS | 45.0% | |
| TTCATTCAGAGCCAGAGGTA+TGG | - | Chr3:63464869-63464888 | MsG0380014974.01.T01:CDS | 45.0% | |
| CGCCTACATCAATCCCACAT+AGG | + | Chr3:63464831-63464850 | None:intergenic | 50.0% | |
| CTTTGTACCTCCAATGGGGA+AGG | + | Chr3:63464895-63464914 | None:intergenic | 50.0% | |
| GGACTTCTCCATCTCCCAAT+TGG | + | Chr3:63465040-63465059 | None:intergenic | 50.0% | |
| ! | CTTCCCCATTGGAGGTACAA+AGG | - | Chr3:63464893-63464912 | MsG0380014974.01.T01:CDS | 50.0% |
| AATGGGGAAGGCCATACCTC+TGG | + | Chr3:63464883-63464902 | None:intergenic | 55.0% | |
| GGAGAAGGCCAATTGGGAGA+TGG | - | Chr3:63465029-63465048 | MsG0380014974.01.T01:CDS | 55.0% | |
| ! | AGAGGTATGGCCTTCCCCAT+TGG | - | Chr3:63464882-63464901 | MsG0380014974.01.T01:CDS | 55.0% |
| GGTATGGCCTTCCCCATTGG+AGG | - | Chr3:63464885-63464904 | MsG0380014974.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 63464721 | 63465206 | 63464721 | ID=MsG0380014974.01;Name=MsG0380014974.01 |
| Chr3 | mRNA | 63464721 | 63465206 | 63464721 | ID=MsG0380014974.01.T01;Parent=MsG0380014974.01;Name=MsG0380014974.01.T01;_AED=0.19;_eAED=0.19;_QI=0|-1|0|1|-1|1|1|0|161 |
| Chr3 | exon | 63464721 | 63465206 | 63464721 | ID=MsG0380014974.01.T01:exon:29727;Parent=MsG0380014974.01.T01 |
| Chr3 | CDS | 63464721 | 63465206 | 63464721 | ID=MsG0380014974.01.T01:cds;Parent=MsG0380014974.01.T01 |
| Gene Sequence |
| Protein sequence |
