AlfalfaGEDB | MsG0380014978.01

M. sativa cultivar ZhongmuNo.1 / MsG0380014978.01

KEGG: -
GO: GO:0000982 GO:0000987 GO:0003677 GO:0045944 GO:0046983
PFAM: PF00319

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014978.01.T01	PNX98523.1	70.122	164	49	0	1	164	1	164	2.91E-71	222

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014978.01.T01	Q9FJK3	45.161	155	83	2	1	154	1	154	1.73E-31	118

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014978.01.T01	A0A2K3N650	70.122	164	49	0	1	164	1	164	1.39e-71	222

TFs/TRs:

Gene ID	Type	Classification
MsG0380014978.01.T01	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014978.01.T01	MTR_2g035580	66.456	158	53	0	1	158	1	158	1.10e-63	193
MsG0380014978.01.T01	MTR_3g467080	49.689	161	80	1	1	160	1	161	8.36e-48	153
MsG0380014978.01.T01	MTR_3g466980	46.667	165	88	0	1	165	1	165	1.98e-47	154
MsG0380014978.01.T01	MTR_4g063790	48.214	168	84	2	1	165	1	168	2.12e-45	147
MsG0380014978.01.T01	MTR_2g016210	44.970	169	86	3	1	164	1	167	3.23e-39	133
MsG0380014978.01.T01	MTR_3g031100	44.512	164	90	1	1	163	1	164	7.16e-38	130
MsG0380014978.01.T01	MTR_5g075380	35.948	153	98	0	1	153	1	153	6.10e-36	122
MsG0380014978.01.T01	MTR_3g065100	44.805	154	85	0	1	154	1	154	6.13e-36	125
MsG0380014978.01.T01	MTR_3g465410	46.269	134	71	1	29	161	1	134	2.09e-34	118
MsG0380014978.01.T01	MTR_4g032620	37.662	154	95	1	1	153	1	154	4.45e-32	115
MsG0380014978.01.T01	MTR_4g031910	41.463	164	95	1	1	163	1	164	2.26e-31	113
MsG0380014978.01.T01	MTR_3g031240	40.491	163	94	2	1	160	1	163	6.51e-31	110
MsG0380014978.01.T01	MTR_3g466830	40.411	146	87	0	7	152	6	151	8.10e-31	109
MsG0380014978.01.T01	MTR_8g036130	38.554	166	99	2	1	163	1	166	1.02e-29	109
MsG0380014978.01.T01	MTR_1g090697	34.615	156	101	1	1	156	1	155	8.94e-27	99.4
MsG0380014978.01.T01	MTR_4g032260	40.000	155	92	1	1	154	1	155	1.69e-25	96.3
MsG0380014978.01.T01	MTR_5g047580	35.065	154	99	1	1	154	1	153	8.35e-25	94.4
MsG0380014978.01.T01	MTR_1g077390	33.987	153	100	1	1	153	1	152	2.41e-24	93.2
MsG0380014978.01.T01	MTR_4g032290	39.873	158	94	1	7	163	7	164	6.36e-24	94.0
MsG0380014978.01.T01	MTR_4g028720	37.908	153	94	1	1	153	1	152	6.88e-24	92.0
MsG0380014978.01.T01	MTR_1g090783	32.895	152	101	1	7	158	6	156	2.29e-23	90.5
MsG0380014978.01.T01	MTR_7g011950	35.948	153	97	1	1	153	1	152	1.45e-22	88.6
MsG0380014978.01.T01	MTR_3g466890	39.597	149	78	5	7	149	6	148	2.08e-22	88.2
MsG0380014978.01.T01	MTR_1g077320	33.987	153	100	1	1	153	1	152	2.48e-22	87.8
MsG0380014978.01.T01	MTR_1g084950	37.013	154	96	1	1	154	1	153	1.66e-21	85.9
MsG0380014978.01.T01	MTR_2g035610	37.190	121	76	0	1	121	1	121	2.29e-21	84.3
MsG0380014978.01.T01	MTR_1g090710	32.692	156	101	3	1	154	1	154	2.66e-20	82.4
MsG0380014978.01.T01	MTR_3g466930	38.760	129	65	5	32	153	2	123	5.37e-16	70.9
MsG0380014978.01.T01	MTR_3g466900	34.146	123	79	2	32	153	2	123	9.34e-16	70.1
MsG0380014978.01.T01	MTR_4g028800	40.000	70	40	1	1	70	1	68	2.87e-14	64.7
MsG0380014978.01.T01	MTR_1g077300	33.333	153	99	2	1	153	1	150	8.41e-14	65.5
MsG0380014978.01.T01	MTR_6g018920	37.383	107	57	4	8	105	8	113	4.90e-12	63.2
MsG0380014978.01.T01	MTR_4g019670	28.462	130	81	3	4	128	5	127	1.42e-11	62.0
MsG0380014978.01.T01	MTR_5g047560	27.273	154	95	2	1	154	1	137	3.61e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014978.01.T01	AT5G48670	45.161	155	83	2	1	154	1	154	1.76e-32	118
MsG0380014978.01.T01	AT3G05860	39.597	149	90	0	1	149	1	149	4.40e-31	111
MsG0380014978.01.T01	AT3G05860	39.597	149	90	0	1	149	1	149	1.39e-30	111
MsG0380014978.01.T01	AT3G05860	39.597	149	90	0	1	149	1	149	1.72e-30	111
MsG0380014978.01.T01	AT1G65330	39.490	157	93	2	7	162	6	161	1.13e-29	110
MsG0380014978.01.T01	AT1G65300	35.849	159	102	0	4	162	3	161	3.15e-25	98.2
MsG0380014978.01.T01	AT5G27810	46.067	89	48	0	29	117	1	89	1.28e-22	87.4
MsG0380014978.01.T01	AT1G31640	34.591	159	99	2	6	162	5	160	7.29e-21	88.6
MsG0380014978.01.T01	AT1G22590	29.747	158	108	1	1	158	1	155	4.02e-19	79.7
MsG0380014978.01.T01	AT1G31630	31.333	150	96	2	7	153	6	151	6.34e-19	82.4
MsG0380014978.01.T01	AT5G27960	32.051	156	106	0	7	162	5	160	7.97e-19	82.0
MsG0380014978.01.T01	AT5G26650	32.051	156	106	0	7	162	5	160	1.16e-18	82.0
MsG0380014978.01.T01	AT2G28700	35.922	103	66	0	1	103	1	103	2.34e-18	80.9
MsG0380014978.01.T01	AT5G26630	29.630	162	114	0	1	162	1	162	1.42e-15	71.6
MsG0380014978.01.T01	AT5G06500	32.308	130	80	3	1	126	1	126	1.16e-13	67.0
MsG0380014978.01.T01	AT2G40210	28.058	139	99	1	1	139	1	138	2.11e-11	61.6
MsG0380014978.01.T01	AT5G26580	28.395	162	102	3	1	162	1	148	2.88e-11	60.8

Find 33 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACCATCTGGACTTCTTCTT+TGG	0.146388	3:+63525948	None:intergenic
GAAAGGCAACATTTGGAAAA+AGG	0.291917	3:-63526286	MsG0380014978.01.T01:CDS
CTCATTCATTTCATTCATCT+TGG	0.326863	3:+63525804	None:intergenic
TTCTTTGTTCGACTTCCTTA+AGG	0.340784	3:+63525692	None:intergenic
TTTGTTTCTTTAACTTCTCT+AGG	0.340953	3:+63525836	None:intergenic
ATTCATTCATACGGGTGAAT+TGG	0.359950	3:-63525770	MsG0380014978.01.T01:CDS
TCGAAGAGAAAGGCAACATT+TGG	0.402575	3:-63526293	MsG0380014978.01.T01:CDS
TGAACTTGTCCAACACCATC+TGG	0.408010	3:+63525935	None:intergenic
AATGAAATGAATGAGCTAAT+TGG	0.431503	3:-63525796	MsG0380014978.01.T01:CDS
ATCTGGACTTCTTCTTTGGA+TGG	0.436613	3:+63525952	None:intergenic
TTGTTTCTTTAACTTCTCTA+GGG	0.453550	3:+63525837	None:intergenic
AATTGGTCAATTCATTCATA+CGG	0.459890	3:-63525779	MsG0380014978.01.T01:CDS
GCTTATTGTTGAGAACCTTA+AGG	0.479647	3:-63525707	MsG0380014978.01.T01:CDS
ACATACATCAATTCCACATA+AGG	0.491397	3:+63526017	None:intergenic
AATGAAATCAGCACCTTATG+TGG	0.499973	3:-63526030	MsG0380014978.01.T01:CDS
GGGTGAATTGGATGGAAATG+TGG	0.509708	3:-63525758	MsG0380014978.01.T01:CDS
AGAGAAGTTAAAGAAACAAA+AGG	0.515728	3:-63525833	MsG0380014978.01.T01:CDS
ATTCATACGGGTGAATTGGA+TGG	0.523018	3:-63525766	MsG0380014978.01.T01:CDS
AACTGATGACTCGAAGAGAA+AGG	0.544142	3:-63526303	MsG0380014978.01.T01:CDS
TTTCGAATTTCTAGCAGCCA+TGG	0.551648	3:+63526337	None:intergenic
AAAATCGATGGTGGATCATG+AGG	0.552677	3:-63525887	MsG0380014978.01.T01:CDS
ATTGGTCAATTCATTCATAC+GGG	0.554460	3:-63525778	MsG0380014978.01.T01:CDS
AAGTCGAACAAAGAATCGAA+TGG	0.588974	3:-63525684	MsG0380014978.01.T01:CDS
AGAAGAAGTCCAGATGGTGT+TGG	0.590630	3:-63525944	MsG0380014978.01.T01:CDS
GGTGAATTGGATGGAAATGT+GGG	0.594645	3:-63525757	MsG0380014978.01.T01:CDS
ATTTGAACAAAGAAAATCGA+TGG	0.602892	3:-63525899	MsG0380014978.01.T01:CDS
AAATCGATGGTGGATCATGA+GGG	0.613825	3:-63525886	MsG0380014978.01.T01:CDS
TGTATGTGGTATAATCTATG+AGG	0.618109	3:-63526001	MsG0380014978.01.T01:CDS
TGAACAAAGAAAATCGATGG+TGG	0.625312	3:-63525896	MsG0380014978.01.T01:CDS
ATCCAAAGAAGAAGTCCAGA+TGG	0.628220	3:-63525950	MsG0380014978.01.T01:CDS
GGAAAACAATGCTCAAGCAA+TGG	0.639574	3:-63525980	MsG0380014978.01.T01:CDS
TTATGTGGAATTGATGTATG+TGG	0.644295	3:-63526015	MsG0380014978.01.T01:CDS
TGGATCATGAGGGCTTCTTG+AGG	0.648158	3:-63525876	MsG0380014978.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTTGCTTCATAAAAATTA+AGG	+	Chr3:63525742-63525761	None:intergenic	15.0%
!!	ACTTTATACTATAAATGACA+TGG	+	Chr3:63525864-63525883	None:intergenic	20.0%
!!	TGATGTTCATAAATTTCTAA+TGG	+	Chr3:63525782-63525801	None:intergenic	20.0%
!	AATGAAATGAATGAGCTAAT+TGG	-	Chr3:63526181-63526200	MsG0380014978.01.T01:intron	25.0%
!	AATTGGTCAATTCATTCATA+CGG	-	Chr3:63526198-63526217	MsG0380014978.01.T01:intron	25.0%
!	AGAGAAGTTAAAGAAACAAA+AGG	-	Chr3:63526144-63526163	MsG0380014978.01.T01:intron	25.0%
!	ATTTGAACAAAGAAAATCGA+TGG	-	Chr3:63526078-63526097	MsG0380014978.01.T01:intron	25.0%
!!	GATTTTCTTTGTTCAAATTC+AGG	+	Chr3:63526076-63526095	None:intergenic	25.0%
!!	TCAATTTTACATTTCTCTGA+CGG	-	Chr3:63525885-63525904	MsG0380014978.01.T01:CDS	25.0%
!!	TTGTTTCTTTAACTTCTCTA+GGG	+	Chr3:63526143-63526162	None:intergenic	25.0%
!!	TTTGTTTCTTTAACTTCTCT+AGG	+	Chr3:63526144-63526163	None:intergenic	25.0%
!!!	TTCATTTTGAGATTCATCTT+GGG	+	Chr3:63526326-63526345	None:intergenic	25.0%
	ACATACATCAATTCCACATA+AGG	+	Chr3:63525963-63525982	None:intergenic	30.0%
	ACATTTGGAAAAAGGAAACA+AGG	-	Chr3:63525699-63525718	MsG0380014978.01.T01:CDS	30.0%
	ATTGGTCAATTCATTCATAC+GGG	-	Chr3:63526199-63526218	MsG0380014978.01.T01:intron	30.0%
	CTCATTCATTTCATTCATCT+TGG	+	Chr3:63526176-63526195	None:intergenic	30.0%
	TGTATGTGGTATAATCTATG+AGG	-	Chr3:63525976-63525995	MsG0380014978.01.T01:CDS	30.0%
	TTATGTGGAATTGATGTATG+TGG	-	Chr3:63525962-63525981	MsG0380014978.01.T01:CDS	30.0%
!!!	CTTCATTTTGAGATTCATCT+TGG	+	Chr3:63526327-63526346	None:intergenic	30.0%
	AAGTCGAACAAAGAATCGAA+TGG	-	Chr3:63526293-63526312	MsG0380014978.01.T01:CDS	35.0%
	AATGAAATCAGCACCTTATG+TGG	-	Chr3:63525947-63525966	MsG0380014978.01.T01:CDS	35.0%
	ATTCATTCATACGGGTGAAT+TGG	-	Chr3:63526207-63526226	MsG0380014978.01.T01:intron	35.0%
	GAAAGGCAACATTTGGAAAA+AGG	-	Chr3:63525691-63525710	MsG0380014978.01.T01:CDS	35.0%
	GCTTATTGTTGAGAACCTTA+AGG	-	Chr3:63526270-63526289	MsG0380014978.01.T01:intron	35.0%
	TGAACAAAGAAAATCGATGG+TGG	-	Chr3:63526081-63526100	MsG0380014978.01.T01:intron	35.0%
	TTCTTTGTTCGACTTCCTTA+AGG	+	Chr3:63526288-63526307	None:intergenic	35.0%
!!	CTCTTGCTATGTTTTCAAAC+AGG	-	Chr3:63525908-63525927	MsG0380014978.01.T01:CDS	35.0%
	AACTGATGACTCGAAGAGAA+AGG	-	Chr3:63525674-63525693	MsG0380014978.01.T01:CDS	40.0%
	ATCCAAAGAAGAAGTCCAGA+TGG	-	Chr3:63526027-63526046	MsG0380014978.01.T01:CDS	40.0%
	ATCTGGACTTCTTCTTTGGA+TGG	+	Chr3:63526028-63526047	None:intergenic	40.0%
	ATTCATACGGGTGAATTGGA+TGG	-	Chr3:63526211-63526230	MsG0380014978.01.T01:intron	40.0%
	GGAAAACAATGCTCAAGCAA+TGG	-	Chr3:63525997-63526016	MsG0380014978.01.T01:CDS	40.0%
	GGTGAATTGGATGGAAATGT+GGG	-	Chr3:63526220-63526239	MsG0380014978.01.T01:intron	40.0%
	TCGAAGAGAAAGGCAACATT+TGG	-	Chr3:63525684-63525703	MsG0380014978.01.T01:CDS	40.0%
!!	AAAATCGATGGTGGATCATG+AGG	-	Chr3:63526090-63526109	MsG0380014978.01.T01:intron	40.0%
!!	AAATCGATGGTGGATCATGA+GGG	-	Chr3:63526091-63526110	MsG0380014978.01.T01:intron	40.0%
	AGAAGAAGTCCAGATGGTGT+TGG	-	Chr3:63526033-63526052	MsG0380014978.01.T01:CDS	45.0%
	CACCATCTGGACTTCTTCTT+TGG	+	Chr3:63526032-63526051	None:intergenic	45.0%
	GGGTGAATTGGATGGAAATG+TGG	-	Chr3:63526219-63526238	MsG0380014978.01.T01:intron	45.0%
	TGAACTTGTCCAACACCATC+TGG	+	Chr3:63526045-63526064	None:intergenic	45.0%
!!!	CTTCTCTAGGGCTTTTGCTA+TGG	+	Chr3:63526131-63526150	None:intergenic	45.0%
	TGGATCATGAGGGCTTCTTG+AGG	-	Chr3:63526101-63526120	MsG0380014978.01.T01:intron	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	63525642	63526357	63525642	ID=MsG0380014978.01;Name=MsG0380014978.01
Chr3	mRNA	63525642	63526357	63525642	ID=MsG0380014978.01.T01;Parent=MsG0380014978.01;Name=MsG0380014978.01.T01;_AED=0.53;_eAED=0.53;_QI=0\|0\|0\|0.5\|1\|1\|2\|0\|168
Chr3	exon	63526279	63526357	63526279	ID=MsG0380014978.01.T01:exon:6690;Parent=MsG0380014978.01.T01
Chr3	exon	63525642	63526069	63525642	ID=MsG0380014978.01.T01:exon:6689;Parent=MsG0380014978.01.T01
Chr3	CDS	63526279	63526357	63526279	ID=MsG0380014978.01.T01:cds;Parent=MsG0380014978.01.T01
Chr3	CDS	63525642	63526069	63525642	ID=MsG0380014978.01.T01:cds;Parent=MsG0380014978.01.T01

Gene Sequence

>MsG0380014978.01.T01

ATGGCTGCTAGAAATTCGAAAATTGAATACATAACTGATGACTCGAAGAGAAAGGCAACATTTGGAAAAAGGAAACAAGGCATAATTAAGAAGATTAATGAAATCAGCACCTTATGTGGAATTGATGTATGTGGTATAATCTATGAGGAAAACAATGCTCAAGCAATGGTTTGTCCATCCAAAGAAGAAGTCCAGATGGTGTTGGACAAGTTCATGAGTTTTCCTGAATTTGAACAAAGAAAATCGATGGTGGATCATGAGGGCTTCTTGAGGAAATCCATAGCAAAAGCCCTAGAGAAGTTAAAGAAACAAAAGGACAACTCCAAGATGAATGAAATGAATGAGCTAATTGGTCAATTCATTCATACGGGTGAATTGGATGGAAATGTGGGTGAGAGTGATCTTGAAGATCTGTTGTTGCTTATTGTTGAGAACCTTAAGGAAGTCGAACAAAGAATCGAATGGATTCAAGCCCAAGATGAATCTCAAAATGAAGATTTGAGATAA

Protein sequence

>MsG0380014978.01.T01

MAARNSKIEYITDDSKRKATFGKRKQGIIKKINEISTLCGIDVCGIIYEENNAQAMVCPSKEEVQMVLDKFMSFPEFEQRKSMVDHEGFLRKSIAKALEKLKKQKDNSKMNEMNELIGQFIHTGELDGNVGESDLEDLLLLIVENLKEVEQRIEWIQAQDESQNEDLR*