AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380015337.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380015337.01.T01 MTR_3g067910 87.963 108 13 0 17 124 2 109 5.67e-67 197
MsG0380015337.01.T01 MTR_5g045560 80.342 117 23 0 2 118 6 122 4.17e-62 190
MsG0380015337.01.T01 MTR_2g085280 73.171 123 33 0 2 124 6 128 5.91e-61 187
MsG0380015337.01.T01 MTR_2g085250 73.171 123 33 0 2 124 6 128 5.98e-61 187
MsG0380015337.01.T01 MTR_3g052870 73.729 118 31 0 2 119 6 123 2.44e-59 182
MsG0380015337.01.T01 MTR_1g105920 73.729 118 31 0 2 119 6 123 4.54e-59 182
MsG0380015337.01.T01 MTR_1g077360 72.650 117 32 0 2 118 6 122 3.94e-58 179
MsG0380015337.01.T01 MTR_8g043650 72.034 118 33 0 2 119 6 123 8.00e-58 179
MsG0380015337.01.T01 MTR_1g075600 73.729 118 31 0 2 119 6 123 2.46e-57 177
MsG0380015337.01.T01 MTR_5g055100 72.881 118 32 0 2 119 6 123 1.17e-56 176
MsG0380015337.01.T01 MTR_1g105910 72.174 115 32 0 2 116 6 120 1.75e-56 176
MsG0380015337.01.T01 MTR_5g053390 71.774 124 34 1 2 124 6 129 1.06e-55 173
MsG0380015337.01.T01 MTR_1g106070 69.492 118 36 0 2 119 6 123 1.58e-55 171
MsG0380015337.01.T01 MTR_2g030740 70.339 118 35 0 2 119 6 123 2.01e-55 173
MsG0380015337.01.T01 MTR_3g052920 70.339 118 35 0 2 119 6 123 3.42e-55 172
MsG0380015337.01.T01 MTR_1g108510 68.644 118 37 0 2 119 6 123 2.08e-53 167
MsG0380015337.01.T01 MTR_1g012570 71.186 118 34 0 2 119 6 123 2.89e-53 167
MsG0380015337.01.T01 MTR_3g030770 68.644 118 37 0 2 119 6 123 3.58e-53 167
MsG0380015337.01.T01 MTR_1g108580 66.949 118 39 0 2 119 6 123 2.33e-52 165
MsG0380015337.01.T01 MTR_2g093190 66.949 118 39 0 2 119 6 123 3.42e-50 159
MsG0380015337.01.T01 MTR_0121s0100 64.407 118 42 0 2 119 6 123 1.33e-49 154
MsG0380015337.01.T01 MTR_3g030780 61.864 118 45 0 2 119 6 123 8.63e-49 152
MsG0380015337.01.T01 MTR_0121s0080 63.559 118 43 0 2 119 6 123 8.51e-47 150
MsG0380015337.01.T01 MTR_1g105905 65.714 105 36 0 14 118 1 105 4.50e-44 143
MsG0380015337.01.T01 MTR_3g080940 53.448 116 54 0 5 120 9 124 8.66e-39 129
MsG0380015337.01.T01 MTR_1g075570 62.264 106 26 1 14 119 1 92 1.06e-36 123
MsG0380015337.01.T01 MTR_7g062350 55.263 114 50 1 12 124 27 140 1.54e-34 120
MsG0380015337.01.T01 MTR_3g067915 79.688 64 13 0 56 119 30 93 1.46e-31 107
MsG0380015337.01.T01 MTR_3g067875 85.714 56 8 0 14 69 1 56 5.85e-28 97.8
MsG0380015337.01.T01 MTR_8g086290 49.412 85 36 1 2 86 9 86 3.95e-23 89.0
MsG0380015337.01.T01 MTR_2g105290 54.167 72 33 0 2 73 9 80 5.81e-23 88.6
MsG0380015337.01.T01 MTR_1g108500 46.087 115 39 2 1 113 91 184 1.92e-22 90.1
MsG0380015337.01.T01 MTR_3g067870 69.841 63 19 0 12 74 83 145 3.86e-22 85.9
MsG0380015337.01.T01 MTR_1g047550 50.704 71 35 0 5 75 2 72 1.76e-21 84.7
MsG0380015337.01.T01 MTR_8g079502 50.704 71 35 0 5 75 2 72 4.75e-21 83.6
MsG0380015337.01.T01 MTR_7g028448 49.315 73 37 0 18 90 25 97 5.05e-21 83.2
MsG0380015337.01.T01 MTR_4g036915 49.315 73 37 0 18 90 25 97 5.05e-21 83.2
MsG0380015337.01.T01 MTR_8g051580 45.570 79 43 0 5 83 2 80 2.78e-20 81.6
MsG0380015337.01.T01 MTR_1g054265 45.570 79 43 0 5 83 2 80 1.09e-19 79.7
MsG0380015337.01.T01 MTR_1g063160 43.038 79 45 0 5 83 2 80 1.36e-19 79.7
MsG0380015337.01.T01 MTR_4g131030 43.038 79 45 0 5 83 2 80 2.09e-19 80.1
MsG0380015337.01.T01 MTR_8g046350 45.070 71 39 0 5 75 2 72 2.43e-18 76.3
MsG0380015337.01.T01 MTR_4g127140 55.556 72 32 0 2 73 9 80 1.65e-17 74.7
MsG0380015337.01.T01 MTR_1g053070 49.383 81 41 0 5 85 2 82 9.82e-17 73.6
MsG0380015337.01.T01 MTR_4g094632 37.736 106 64 1 5 110 2 105 1.35e-16 71.6
MsG0380015337.01.T01 MTR_4g102530 46.575 73 39 0 6 78 3 75 1.22e-15 70.1
MsG0380015337.01.T01 MTR_3g005530 47.059 68 36 0 5 72 2 69 1.48e-15 70.1
MsG0380015337.01.T01 MTR_4g093970 50.000 66 33 0 6 71 29 94 1.55e-15 70.5
MsG0380015337.01.T01 MTR_5g066180 46.479 71 38 0 5 75 2 72 2.73e-15 69.3
MsG0380015337.01.T01 MTR_8g033270 50.000 68 34 0 5 72 2 69 5.27e-15 68.9
MsG0380015337.01.T01 MTR_0003s0590 50.000 68 34 0 5 72 2 69 7.59e-15 68.6
MsG0380015337.01.T01 MTR_7g075870 49.254 67 34 0 6 72 3 69 1.33e-14 67.4
MsG0380015337.01.T01 MTR_4g094638 47.826 69 36 0 5 73 2 70 1.58e-14 66.6
MsG0380015337.01.T01 MTR_1g101970 50.000 68 34 0 5 72 2 69 2.36e-14 66.2
MsG0380015337.01.T01 MTR_3g452380 45.070 71 39 0 2 72 14 84 2.66e-14 67.0
MsG0380015337.01.T01 MTR_2g009890 43.421 76 43 0 5 80 2 77 2.92e-14 67.4
MsG0380015337.01.T01 MTR_6g464720 49.275 69 34 1 5 72 2 70 3.06e-14 67.0
MsG0380015337.01.T01 MTR_8g087860 47.059 68 36 0 5 72 18 85 3.24e-14 67.0
MsG0380015337.01.T01 MTR_2g017865 47.059 68 36 0 5 72 17 84 4.62e-14 66.6
MsG0380015337.01.T01 MTR_8g033220 46.377 69 37 0 6 74 3 71 5.77e-14 65.9
MsG0380015337.01.T01 MTR_8g033220 46.377 69 37 0 6 74 3 71 5.87e-14 65.9
MsG0380015337.01.T01 MTR_7g075870 48.529 68 34 1 6 72 3 70 1.78e-13 64.3
MsG0380015337.01.T01 MTR_4g084740 46.053 76 39 2 5 79 2 76 2.27e-13 65.5
MsG0380015337.01.T01 MTR_5g031000 45.588 68 37 0 5 72 2 69 2.38e-13 64.3
MsG0380015337.01.T01 MTR_4g109830 46.377 69 36 1 5 72 2 70 2.51e-13 64.3
MsG0380015337.01.T01 MTR_5g031000 45.588 68 37 0 5 72 2 69 2.69e-13 64.3
MsG0380015337.01.T01 MTR_5g032520 36.792 106 58 2 6 104 3 106 3.36e-13 63.9
MsG0380015337.01.T01 MTR_3g102570 42.045 88 49 2 5 91 2 88 3.70e-13 64.7
MsG0380015337.01.T01 MTR_5g032150 46.269 67 36 0 6 72 3 69 3.93e-13 63.9
MsG0380015337.01.T01 MTR_7g016600 47.826 69 35 1 5 72 2 70 4.83e-13 63.9
MsG0380015337.01.T01 MTR_5g046870 47.826 69 35 1 5 72 2 70 5.69e-13 61.2
MsG0380015337.01.T01 MTR_5g046790 44.928 69 37 1 5 72 2 70 6.10e-13 63.2
MsG0380015337.01.T01 MTR_2g461710 50.000 56 28 0 5 60 2 57 1.59e-12 58.5
MsG0380015337.01.T01 MTR_8g066260 43.478 69 38 1 5 72 2 70 1.81e-12 62.0
MsG0380015337.01.T01 MTR_1g038300 45.714 70 36 1 5 72 2 71 1.93e-12 62.0
MsG0380015337.01.T01 MTR_5g066960 45.763 59 32 0 5 63 2 60 2.36e-12 58.2
MsG0380015337.01.T01 MTR_7g016630 40.278 72 42 1 5 75 2 73 9.14e-12 60.5
MsG0380015337.01.T01 MTR_4g109810 49.153 59 30 0 5 63 2 60 1.22e-11 59.7
MsG0380015337.01.T01 MTR_6g015975 44.928 69 37 1 5 72 2 70 1.70e-11 59.7
MsG0380015337.01.T01 MTR_8g097090 44.928 69 37 1 5 72 2 70 2.27e-11 59.3
MsG0380015337.01.T01 MTR_3g084980 48.333 60 31 0 5 64 2 61 2.58e-11 58.9
MsG0380015337.01.T01 MTR_7g075850 44.286 70 37 1 5 72 2 71 2.66e-11 58.9
MsG0380015337.01.T01 MTR_1g029670 48.214 56 29 0 5 60 2 57 3.85e-11 57.8
MsG0380015337.01.T01 MTR_3g088615 41.429 70 39 1 5 72 2 71 3.91e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380015337.01.T01 AT5G60440 57.025 121 50 1 2 120 4 124 2.01e-40 136
MsG0380015337.01.T01 AT4G36590 54.630 108 44 1 3 105 5 112 4.07e-30 108
MsG0380015337.01.T01 AT2G24840 46.610 118 57 2 5 120 63 176 4.85e-27 101
MsG0380015337.01.T01 AT1G65360 48.214 112 56 1 2 113 4 113 7.00e-27 99.8
MsG0380015337.01.T01 AT1G01530 50.000 104 50 1 2 105 4 105 1.31e-26 99.8
MsG0380015337.01.T01 AT2G34440 39.640 111 66 1 5 115 2 111 1.89e-22 87.0
MsG0380015337.01.T01 AT3G04100 35.043 117 71 2 2 113 11 127 7.32e-20 81.3
MsG0380015337.01.T01 AT3G66656 51.471 68 33 0 5 72 2 69 1.58e-18 77.0
MsG0380015337.01.T01 AT1G72350 50.685 73 34 1 5 77 43 113 1.76e-18 77.8
MsG0380015337.01.T01 AT2G45650 52.941 68 32 0 5 72 2 69 5.56e-17 74.3
MsG0380015337.01.T01 AT2G22540 42.308 104 55 1 6 104 3 106 6.11e-17 74.3
MsG0380015337.01.T01 AT2G22540 42.308 104 55 1 6 104 3 106 6.11e-17 74.3
MsG0380015337.01.T01 AT2G45660 48.529 68 35 0 6 73 3 70 1.97e-16 71.2
MsG0380015337.01.T01 AT4G22950 44.737 76 42 0 6 81 3 78 3.62e-16 70.9
MsG0380015337.01.T01 AT2G45660 48.529 68 35 0 6 73 3 70 5.04e-16 71.2
MsG0380015337.01.T01 AT4G22950 46.479 71 38 0 6 76 3 73 1.29e-15 70.1
MsG0380015337.01.T01 AT4G22950 46.479 71 38 0 6 76 3 73 1.29e-15 70.1
MsG0380015337.01.T01 AT4G09960 47.059 68 36 0 5 72 2 69 1.48e-15 70.1
MsG0380015337.01.T01 AT4G09960 45.833 72 39 0 1 72 8 79 1.59e-15 70.5
MsG0380015337.01.T01 AT4G09960 47.059 68 36 0 5 72 3 70 1.83e-15 70.1
MsG0380015337.01.T01 AT4G09960 47.059 68 36 0 5 72 2 69 1.88e-15 70.1
MsG0380015337.01.T01 AT4G09960 47.059 68 36 0 5 72 28 95 3.05e-15 69.7
MsG0380015337.01.T01 AT5G13790 48.529 68 35 0 5 72 2 69 3.37e-15 70.1
MsG0380015337.01.T01 AT1G77950 46.053 76 39 2 5 79 2 76 3.55e-15 69.7
MsG0380015337.01.T01 AT1G77950 46.053 76 39 2 5 79 2 76 3.55e-15 69.7
MsG0380015337.01.T01 AT1G77950 46.053 76 39 2 5 79 2 76 3.55e-15 69.7
MsG0380015337.01.T01 AT1G77950 46.053 76 39 2 5 79 2 76 3.55e-15 69.7
MsG0380015337.01.T01 AT5G13790 48.529 68 35 0 5 72 2 69 3.69e-15 69.7
MsG0380015337.01.T01 AT4G09960 47.059 68 36 0 5 72 97 164 4.50e-15 70.1
MsG0380015337.01.T01 AT3G61120 51.471 68 33 0 5 72 2 69 4.63e-15 69.3
MsG0380015337.01.T01 AT5G62165 47.761 67 35 0 6 72 3 69 5.16e-15 66.2
MsG0380015337.01.T01 AT5G51870 49.254 67 34 0 6 72 3 69 6.06e-15 67.8
MsG0380015337.01.T01 AT5G51870 47.887 71 37 0 2 72 22 92 6.19e-15 68.2
MsG0380015337.01.T01 AT5G51870 49.254 67 34 0 6 72 3 69 6.63e-15 68.2
MsG0380015337.01.T01 AT1G77980 42.045 88 50 1 5 91 2 89 7.77e-15 69.3
MsG0380015337.01.T01 AT5G51870 49.254 67 34 0 6 72 3 69 7.77e-15 68.2
MsG0380015337.01.T01 AT5G51860 49.254 67 34 0 6 72 3 69 8.66e-15 67.8
MsG0380015337.01.T01 AT5G62165 47.761 67 35 0 6 72 3 69 8.72e-15 67.0
MsG0380015337.01.T01 AT5G62165 47.761 67 35 0 6 72 3 69 8.72e-15 67.0
MsG0380015337.01.T01 AT5G62165 47.761 67 35 0 6 72 3 69 8.72e-15 67.0
MsG0380015337.01.T01 AT3G57390 50.000 68 34 0 5 72 2 69 9.55e-15 68.2
MsG0380015337.01.T01 AT5G51870 49.254 67 34 0 6 72 13 79 1.05e-14 68.2
MsG0380015337.01.T01 AT4G24540 48.529 68 35 0 5 72 2 69 1.23e-14 67.8
MsG0380015337.01.T01 AT5G51860 49.254 67 34 0 6 72 3 69 1.29e-14 67.4
MsG0380015337.01.T01 AT3G57390 50.000 68 34 0 5 72 2 69 1.30e-14 68.2
MsG0380015337.01.T01 AT1G22130 40.217 92 54 1 5 95 2 93 1.31e-14 68.9
MsG0380015337.01.T01 AT3G57230 45.588 68 37 0 5 72 2 69 1.43e-14 65.9
MsG0380015337.01.T01 AT5G62165 47.761 67 35 0 6 72 3 69 1.73e-14 65.9
MsG0380015337.01.T01 AT5G62165 47.761 67 35 0 6 72 3 69 1.73e-14 65.9
MsG0380015337.01.T01 AT5G62165 47.761 67 35 0 6 72 3 69 1.73e-14 65.9
MsG0380015337.01.T01 AT5G62165 47.761 67 35 0 6 72 3 69 1.73e-14 65.9
MsG0380015337.01.T01 AT5G62165 47.761 67 35 0 6 72 3 69 1.73e-14 65.9
MsG0380015337.01.T01 AT2G22540 41.346 104 51 2 6 104 3 101 1.76e-14 67.4
MsG0380015337.01.T01 AT4G18960 40.741 81 48 0 1 81 14 94 1.77e-14 67.8
MsG0380015337.01.T01 AT5G62165 47.761 67 35 0 6 72 3 69 2.22e-14 66.6
MsG0380015337.01.T01 AT5G62165 47.761 67 35 0 6 72 3 69 2.22e-14 66.6
MsG0380015337.01.T01 AT5G62165 47.761 67 35 0 6 72 3 69 2.34e-14 66.6
MsG0380015337.01.T01 AT5G62165 47.761 67 35 0 6 72 3 69 2.34e-14 66.6
MsG0380015337.01.T01 AT5G62165 47.761 67 35 0 6 72 3 69 2.34e-14 66.6
MsG0380015337.01.T01 AT2G42830 47.059 68 36 0 5 72 17 84 3.78e-14 66.6
MsG0380015337.01.T01 AT2G42830 47.059 68 36 0 5 72 17 84 3.93e-14 66.6
MsG0380015337.01.T01 AT3G58780 46.479 71 38 0 2 72 14 84 4.27e-14 66.6
MsG0380015337.01.T01 AT1G26310 38.835 103 46 3 5 106 2 88 4.56e-14 66.6
MsG0380015337.01.T01 AT3G58780 46.479 71 38 0 2 72 20 90 4.90e-14 66.6
MsG0380015337.01.T01 AT4G37940 45.588 68 37 0 5 72 2 69 5.50e-14 65.1
MsG0380015337.01.T01 AT4G37940 45.588 68 37 0 5 72 2 69 6.75e-14 65.5
MsG0380015337.01.T01 AT3G58780 46.479 71 38 0 2 72 14 84 7.29e-14 66.2
MsG0380015337.01.T01 AT1G69120 35.714 112 55 3 5 115 2 97 7.34e-14 65.9
MsG0380015337.01.T01 AT4G37940 45.588 68 37 0 5 72 2 69 7.71e-14 65.9
MsG0380015337.01.T01 AT3G57230 45.588 68 37 0 5 72 2 69 9.57e-14 65.5
MsG0380015337.01.T01 AT3G57230 45.588 68 37 0 5 72 2 69 9.57e-14 65.5
MsG0380015337.01.T01 AT1G69120 35.714 112 55 3 5 115 2 97 1.48e-13 65.5
MsG0380015337.01.T01 AT3G30260 42.029 69 39 1 5 72 2 70 1.56e-13 65.1
MsG0380015337.01.T01 AT4G11880 45.833 72 38 1 6 76 3 74 2.60e-13 64.3
MsG0380015337.01.T01 AT4G11880 45.833 72 38 1 6 76 3 74 2.60e-13 64.3
MsG0380015337.01.T01 AT1G28460 30.631 111 77 0 2 112 5 115 2.87e-13 63.5
MsG0380015337.01.T01 AT5G10140 43.056 72 40 1 5 75 2 73 3.03e-13 63.2
MsG0380015337.01.T01 AT5G10140 43.056 72 40 1 5 75 2 73 3.19e-13 63.2
MsG0380015337.01.T01 AT4G11880 44.595 74 40 1 6 78 3 76 3.23e-13 63.2
MsG0380015337.01.T01 AT4G11880 44.595 74 40 1 6 78 3 76 3.23e-13 63.2
MsG0380015337.01.T01 AT5G60910 44.928 69 37 1 5 72 2 70 3.54e-13 63.9
MsG0380015337.01.T01 AT5G10140 43.056 72 40 1 5 75 2 73 4.19e-13 63.2
MsG0380015337.01.T01 AT5G10140 43.056 72 40 1 5 75 2 73 5.07e-13 62.8
MsG0380015337.01.T01 AT2G22630 45.588 68 37 0 5 72 2 69 5.55e-13 63.5
MsG0380015337.01.T01 AT2G22630 45.588 68 37 0 5 72 2 69 5.55e-13 63.5
MsG0380015337.01.T01 AT1G17310 48.649 74 36 1 4 77 47 118 5.91e-13 63.2
MsG0380015337.01.T01 AT1G17310 46.835 79 40 1 4 82 58 134 8.65e-13 62.8
MsG0380015337.01.T01 AT2G14210 44.928 69 37 1 5 72 2 70 1.30e-12 62.4
MsG0380015337.01.T01 AT2G14210 44.928 69 37 1 5 72 2 70 1.66e-12 62.4
MsG0380015337.01.T01 AT5G15800 46.377 69 36 1 5 72 2 70 1.96e-12 62.0
MsG0380015337.01.T01 AT5G15800 46.377 69 36 1 5 72 2 70 2.92e-12 62.0
MsG0380015337.01.T01 AT3G02310 44.928 69 37 1 5 72 2 70 5.94e-12 60.1
MsG0380015337.01.T01 AT5G23260 43.662 71 38 1 5 73 2 72 8.30e-12 60.5
MsG0380015337.01.T01 AT5G23260 43.662 71 38 1 5 73 2 72 8.37e-12 60.5
MsG0380015337.01.T01 AT5G23260 43.662 71 38 1 5 73 18 88 9.07e-12 60.5
MsG0380015337.01.T01 AT5G23260 43.662 71 38 1 5 73 18 88 9.12e-12 60.5
MsG0380015337.01.T01 AT2G03710 50.000 56 28 0 5 60 2 57 9.54e-12 59.3
MsG0380015337.01.T01 AT1G29962 29.565 115 78 2 2 113 5 119 1.38e-11 58.9
MsG0380015337.01.T01 AT1G24260 44.928 69 37 1 5 72 2 70 1.91e-11 59.3
MsG0380015337.01.T01 AT1G24260 44.928 69 37 1 5 72 2 70 1.93e-11 59.3
MsG0380015337.01.T01 AT1G24260 44.928 69 37 1 5 72 2 70 1.93e-11 59.3
MsG0380015337.01.T01 AT3G58780 45.070 71 32 1 2 72 14 77 1.94e-11 59.3
MsG0380015337.01.T01 AT2G03710 50.000 56 28 0 5 60 2 57 2.40e-11 59.3
MsG0380015337.01.T01 AT2G03710 50.000 56 28 0 5 60 2 57 2.41e-11 59.3
MsG0380015337.01.T01 AT3G54340 44.444 63 35 0 5 67 2 64 2.56e-11 58.9
MsG0380015337.01.T01 AT3G58780 47.541 61 32 0 2 62 14 74 2.66e-11 59.3
MsG0380015337.01.T01 AT5G65070 40.580 69 40 1 5 72 2 70 2.76e-11 57.4
MsG0380015337.01.T01 AT3G02310 44.928 69 37 1 5 72 2 70 2.81e-11 58.9
MsG0380015337.01.T01 AT5G65050 39.130 69 41 1 5 72 2 70 3.83e-11 57.8
MsG0380015337.01.T01 AT5G65070 40.580 69 40 1 5 72 2 70 3.88e-11 58.2
MsG0380015337.01.T01 AT5G65050 39.130 69 41 1 5 72 2 70 6.36e-11 57.4
MsG0380015337.01.T01 AT5G65050 39.130 69 41 1 5 72 2 70 7.00e-11 57.4
MsG0380015337.01.T01 AT5G65050 39.130 69 41 1 5 72 2 70 7.76e-11 57.0

Find 17 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGCAAGATGCCATTGTTT+TGG 0.345850 3:+68635453 None:intergenic
AGCGAGCTTTGCACCCTTTG+TGG 0.386397 3:-68635581 MsG0380015337.01.T01:CDS
CATAAATCGTTATATCTCTC+AGG 0.481105 3:-68635483 MsG0380015337.01.T01:CDS
AGCAACATATGCACCACAAA+GGG 0.521852 3:+68635568 None:intergenic
GCATCGTTCATATGAGTCAA+TGG 0.541468 3:+68635386 None:intergenic
AATGAGCCTCATTTCGCAGA+TGG 0.559491 3:+68635326 None:intergenic
TGGCCACACAAATGTAGAAA+CGG 0.561072 3:-68635507 MsG0380015337.01.T01:CDS
TATATTCTCACCTGGTGAGA+AGG 0.562933 3:-68635540 MsG0380015337.01.T01:CDS
TTTGAGCCATCTGCGAAATG+AGG 0.587155 3:-68635332 None:intergenic
TGACCGTTTCTACATTTGTG+TGG 0.593761 3:+68635504 None:intergenic
AATGAAAACACCTTCTCACC+AGG 0.598148 3:+68635530 None:intergenic
GCTCTGATTATATTCTCACC+TGG 0.608989 3:-68635548 MsG0380015337.01.T01:CDS
CAGGTCCCACCCCAAAACAA+TGG 0.630259 3:-68635464 MsG0380015337.01.T01:CDS
GAGCAACATATGCACCACAA+AGG 0.640810 3:+68635567 None:intergenic
TCGAGTCGAATGAAAACTCG+AGG 0.674187 3:-68635695 None:intergenic
ATGGCTCAAACTCATTACAA+CGG 0.727817 3:+68635345 None:intergenic
TGGCATCTTGCAATTCATCG+AGG 0.757727 3:-68635444 MsG0380015337.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTTTTTCATCTCAATCTTT+TGG + Chr3:68635354-68635373 None:intergenic 25.0%
CATAAATCGTTATATCTCTC+AGG - Chr3:68635537-68635556 MsG0380015337.01.T01:CDS 30.0%
ATGGCTCAAACTCATTACAA+CGG + Chr3:68635678-68635697 None:intergenic 35.0%
! ATTGCAAGATGCCATTGTTT+TGG + Chr3:68635570-68635589 None:intergenic 35.0%
! TTGCAAGATGCCATTGTTTT+GGG + Chr3:68635569-68635588 None:intergenic 35.0%
AATGAAAACACCTTCTCACC+AGG + Chr3:68635493-68635512 None:intergenic 40.0%
AGCAACATATGCACCACAAA+GGG + Chr3:68635455-68635474 None:intergenic 40.0%
GCATCGTTCATATGAGTCAA+TGG + Chr3:68635637-68635656 None:intergenic 40.0%
GCTCTGATTATATTCTCACC+TGG - Chr3:68635472-68635491 MsG0380015337.01.T01:CDS 40.0%
TATATTCTCACCTGGTGAGA+AGG - Chr3:68635480-68635499 MsG0380015337.01.T01:CDS 40.0%
TGACCGTTTCTACATTTGTG+TGG + Chr3:68635519-68635538 None:intergenic 40.0%
TGGCCACACAAATGTAGAAA+CGG - Chr3:68635513-68635532 MsG0380015337.01.T01:CDS 40.0%
!! CTATTTTGAAGAGCCCACTA+TGG + Chr3:68635417-68635436 None:intergenic 40.0%
!! TGCAAGATGCCATTGTTTTG+GGG + Chr3:68635568-68635587 None:intergenic 40.0%
!!! ACTTTTTCGAAGCTCCATAG+TGG - Chr3:68635400-68635419 MsG0380015337.01.T01:CDS 40.0%
!!! CTTTTTCGAAGCTCCATAGT+GGG - Chr3:68635401-68635420 MsG0380015337.01.T01:CDS 40.0%
!!! GGTGAGAAGGTGTTTTCATT+TGG - Chr3:68635493-68635512 MsG0380015337.01.T01:CDS 40.0%
GAGCAACATATGCACCACAA+AGG + Chr3:68635456-68635475 None:intergenic 45.0%
GCTTCGAAAAAGTCACTTGC+AGG + Chr3:68635393-68635412 None:intergenic 45.0%
TGGCATCTTGCAATTCATCG+AGG - Chr3:68635576-68635595 MsG0380015337.01.T01:CDS 45.0%
!! GCTTGCTCACTTTTGCGTTA+CGG + Chr3:68635606-68635625 None:intergenic 45.0%
!!! AAGATGCCATTGTTTTGGGG+TGG + Chr3:68635565-68635584 None:intergenic 45.0%
!!! AGATGCCATTGTTTTGGGGT+GGG + Chr3:68635564-68635583 None:intergenic 45.0%
AGCGAGCTTTGCACCCTTTG+TGG - Chr3:68635439-68635458 MsG0380015337.01.T01:CDS 55.0%
CAGGTCCCACCCCAAAACAA+TGG - Chr3:68635556-68635575 MsG0380015337.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr3 gene 68635334 68635708 68635334 ID=MsG0380015337.01;Name=MsG0380015337.01
Chr3 mRNA 68635334 68635708 68635334 ID=MsG0380015337.01.T01;Parent=MsG0380015337.01;Name=MsG0380015337.01.T01;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|124
Chr3 exon 68635334 68635708 68635334 ID=MsG0380015337.01.T01:exon:23371;Parent=MsG0380015337.01.T01
Chr3 CDS 68635334 68635708 68635334 ID=MsG0380015337.01.T01:cds;Parent=MsG0380015337.01.T01
Gene Sequence

>MsG0380015337.01.T01

ATGAAAACTCGAGGTCGCCAAAAGATTGAGATGAAAAAGATGAGAAATGAAAGCAACCTGCAAGTGACTTTTTCGAAGCTCCATAGTGGGCTCTTCAAAATAGCTAGCGAGCTTTGCACCCTTTGTGGTGCATATGTTGCTCTGATTATATTCTCACCTGGTGAGAAGGTGTTTTCATTTGGCCACACAAATGTAGAAACGGTCATAAATCGTTATATCTCTCAGGTCCCACCCCAAAACAATGGCATCTTGCAATTCATCGAGGCATACCGTAACGCAAAAGTGAGCAAGCTCATTGCTCCATTGACTCATATGAACGATGCACTAGACATCGAGAAGAACCGTTGTAATGAGTTTGAGCCATCTGCGAAATGA
Protein sequence

>MsG0380015337.01.T01

MKTRGRQKIEMKKMRNESNLQVTFSKLHSGLFKIASELCTLCGAYVALIIFSPGEKVFSFGHTNVETVINRYISQVPPQNNGILQFIEAYRNAKVSKLIAPLTHMNDALDIEKNRCNEFEPSAK*