Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015525.01.T01 | XP_003600991.1 | 84.783 | 828 | 103 | 3 | 1 | 821 | 1 | 812 | 0 | 1390 |
| MsG0380015525.01.T01 | XP_003600991.1 | 96.573 | 496 | 17 | 0 | 714 | 1209 | 772 | 1267 | 0 | 981 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015525.01.T01 | MTR_3g071860 | 84.783 | 828 | 103 | 3 | 1 | 821 | 1 | 812 | 0.0 | 1390 |
| MsG0380015525.01.T01 | MTR_3g071860 | 96.573 | 496 | 17 | 0 | 714 | 1209 | 772 | 1267 | 0.0 | 981 |
| MsG0380015525.01.T01 | MTR_7g026650 | 87.578 | 483 | 58 | 2 | 726 | 1208 | 672 | 1152 | 0.0 | 874 |
| MsG0380015525.01.T01 | MTR_7g026650 | 72.468 | 316 | 80 | 2 | 29 | 343 | 9 | 318 | 4.86e-144 | 466 |
| MsG0380015525.01.T01 | MTR_7g026650 | 66.667 | 303 | 85 | 5 | 423 | 724 | 317 | 604 | 1.69e-110 | 374 |
| MsG0380015525.01.T01 | MTR_1g081750 | 81.224 | 490 | 89 | 2 | 719 | 1208 | 629 | 1115 | 0.0 | 810 |
| MsG0380015525.01.T01 | MTR_1g081750 | 65.134 | 261 | 77 | 3 | 1 | 261 | 1 | 247 | 1.54e-96 | 334 |
| MsG0380015525.01.T01 | MTR_1g081750 | 56.231 | 329 | 119 | 8 | 398 | 724 | 263 | 568 | 2.49e-92 | 322 |
| MsG0380015525.01.T01 | MTR_5g083300 | 76.409 | 479 | 106 | 4 | 734 | 1208 | 828 | 1303 | 0.0 | 759 |
| MsG0380015525.01.T01 | MTR_5g083300 | 40.227 | 793 | 357 | 21 | 5 | 724 | 4 | 752 | 7.12e-146 | 474 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015525.01.T01 | AT3G42670 | 70.270 | 481 | 137 | 4 | 730 | 1208 | 778 | 1254 | 0.0 | 700 |
| MsG0380015525.01.T01 | AT3G42670 | 36.607 | 336 | 181 | 9 | 5 | 337 | 3 | 309 | 2.40e-50 | 195 |
| MsG0380015525.01.T01 | AT3G42670 | 34.965 | 286 | 160 | 9 | 453 | 724 | 434 | 707 | 3.57e-34 | 143 |
| MsG0380015525.01.T01 | AT3G42670 | 70.270 | 481 | 137 | 4 | 730 | 1208 | 780 | 1256 | 0.0 | 700 |
| MsG0380015525.01.T01 | AT3G42670 | 36.607 | 336 | 181 | 9 | 5 | 337 | 3 | 309 | 2.63e-50 | 195 |
| MsG0380015525.01.T01 | AT3G42670 | 34.722 | 288 | 160 | 9 | 453 | 724 | 434 | 709 | 5.11e-34 | 142 |
| MsG0380015525.01.T01 | AT3G42670 | 70.270 | 481 | 137 | 4 | 730 | 1208 | 778 | 1254 | 0.0 | 700 |
| MsG0380015525.01.T01 | AT3G42670 | 36.607 | 336 | 181 | 9 | 5 | 337 | 3 | 309 | 2.40e-50 | 195 |
| MsG0380015525.01.T01 | AT3G42670 | 34.965 | 286 | 160 | 9 | 453 | 724 | 434 | 707 | 3.57e-34 | 143 |
| MsG0380015525.01.T01 | AT3G42670 | 70.270 | 481 | 137 | 4 | 730 | 1208 | 778 | 1254 | 0.0 | 700 |
Find 164 sgRNAs with CRISPR-Local
Find 338 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAGATTATGGTACTAAC+AGG | 0.411733 | 3:-71405587 | MsG0380015525.01.T01:CDS |
| AAAGCATTGAGAGAAAGCCC+TGG | 0.610887 | 3:-71406385 | MsG0380015525.01.T01:CDS |
| AAATTGACATGAACATTGAT+CGG | 0.547067 | 3:+71409193 | None:intergenic |
| AAATTTGAAGATCAAAGCAG+CGG | 0.654526 | 3:-71405527 | MsG0380015525.01.T01:CDS |
| AACAAGCATGTTGGAGAAAA+AGG | 0.240380 | 3:-71407980 | MsG0380015525.01.T01:CDS |
| AACACAACCACCACTTGAGT+TGG | 0.495316 | 3:+71406800 | None:intergenic |
| AACCTTGATTTAGTGCTTCT+AGG | 0.271299 | 3:+71406334 | None:intergenic |
| AACTCTATTAAGAATCTCAC+AGG | 0.523720 | 3:+71405641 | None:intergenic |
| AAGAACTACTTGGAAAGAGT+GGG | 0.567137 | 3:-71405300 | MsG0380015525.01.T01:CDS |
| AAGATTGTGTATCAAGTTAT+GGG | 0.381846 | 3:-71408864 | MsG0380015525.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGAGATTATGGTACTAAC+AGG | - | Chr3:71410468-71410487 | MsG0380015525.01.T01:intron | 30.0% | |
| AAATTTGAAGATCAAAGCAG+CGG | - | Chr3:71410528-71410547 | MsG0380015525.01.T01:intron | 30.0% | |
| AACTCTATTAAGAATCTCAC+AGG | + | Chr3:71410417-71410436 | None:intergenic | 30.0% | |
| AAGCTATTAATGTGAACACA+AGG | - | Chr3:71407570-71407589 | MsG0380015525.01.T01:intron | 30.0% | |
| AAGTATTATGTTGAAGCCTT+TGG | + | Chr3:71406986-71407005 | None:intergenic | 30.0% | |
| ACAACATATCCAACAAAATG+AGG | + | Chr3:71408696-71408715 | None:intergenic | 30.0% | |
| ACAAGGAACAAAAATTCTGA+CGG | - | Chr3:71407587-71407606 | MsG0380015525.01.T01:intron | 30.0% | |
| ACAGAAACTTCAGAAGAAAA+TGG | - | Chr3:71410152-71410171 | MsG0380015525.01.T01:intron | 30.0% | |
| ACATCAAGTTATCTTCTTGA+TGG | - | Chr3:71408184-71408203 | MsG0380015525.01.T01:CDS | 30.0% | |
| ACTATCTATTATTTCCTTGC+AGG | + | Chr3:71407990-71408009 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 71405145 | 71410932 | 71405145 | ID=MsG0380015525.01;Name=MsG0380015525.01 |
| Chr3 | mRNA | 71405145 | 71410932 | 71405145 | ID=MsG0380015525.01.T01;Parent=MsG0380015525.01;Name=MsG0380015525.01.T01;_AED=0.20;_eAED=0.20;_QI=0|0|0|1|0.8|0.66|6|0|1209 |
| Chr3 | exon | 71410875 | 71410932 | 71410875 | ID=MsG0380015525.01.T01:exon:23805;Parent=MsG0380015525.01.T01 |
| Chr3 | exon | 71409779 | 71410053 | 71409779 | ID=MsG0380015525.01.T01:exon:23804;Parent=MsG0380015525.01.T01 |
| Chr3 | exon | 71407864 | 71409264 | 71407864 | ID=MsG0380015525.01.T01:exon:23803;Parent=MsG0380015525.01.T01 |
| Chr3 | exon | 71407433 | 71407579 | 71407433 | ID=MsG0380015525.01.T01:exon:23802;Parent=MsG0380015525.01.T01 |
| Chr3 | exon | 71406811 | 71407096 | 71406811 | ID=MsG0380015525.01.T01:exon:23801;Parent=MsG0380015525.01.T01 |
| Chr3 | exon | 71405145 | 71406607 | 71405145 | ID=MsG0380015525.01.T01:exon:23800;Parent=MsG0380015525.01.T01 |
| Chr3 | CDS | 71410875 | 71410932 | 71410875 | ID=MsG0380015525.01.T01:cds;Parent=MsG0380015525.01.T01 |
| Chr3 | CDS | 71409779 | 71410053 | 71409779 | ID=MsG0380015525.01.T01:cds;Parent=MsG0380015525.01.T01 |
| Chr3 | CDS | 71407864 | 71409264 | 71407864 | ID=MsG0380015525.01.T01:cds;Parent=MsG0380015525.01.T01 |
| Chr3 | CDS | 71407433 | 71407579 | 71407433 | ID=MsG0380015525.01.T01:cds;Parent=MsG0380015525.01.T01 |
| Chr3 | CDS | 71406811 | 71407096 | 71406811 | ID=MsG0380015525.01.T01:cds;Parent=MsG0380015525.01.T01 |
| Chr3 | CDS | 71405145 | 71406607 | 71405145 | ID=MsG0380015525.01.T01:cds;Parent=MsG0380015525.01.T01 |
| Gene Sequence |
>MsG0380015525.01.T01 ATGGCGCCCATAAAAAGGCATTTGCATCAATGGAAGCATCCATTCGATGCTAATTATCTTGCTTCCCCTAATTCTTGTTTCAAAACAGCATTTGAAGCAGTATTGAATGGATCATGGCAACCTGTGGAACTCTTAAAAATAGAGTCAGGAAAAACTACCTTACGTTTCATTGATACTGAACAAATTCAAAGCACTTTGTCATCAGATATTCGGATAAGATCAAGGAAAGCTACTTCATCTGATTGCTCCAGCTTTTTACGACCTGGAATTGATATATCTATGCTATTAGGCTATCGATACAATGATAATTCAGATCCATTTACGTCTATTCCAGTGTGGATTGATGCAAGAATAAATTCTATACATAGACAGCCTCACAAATCCGATCAATGTTCATGTCAATTTTATGTTAACTTATATGATGATCAAGGTTCACTTGGTACAGAGGTAAAAACTCTTAATAAAGAGGTTAATGCAATTGAAATAGATCAAATTTTCATCCTCCAAAGGCTTCAACATAATACTTGTGAAGGCTTGTTAAAAGAAAATGATACCTCTGAAATTAAGCCTTATAGGTGGGATTCATCAGAAGATTGCTCTTCATTATCACAATCTAGATTACTCGTTGGGAAATTTTCATCTGATCTTTCGTGGTTTGTAATTACATCTTTTTTAAAGAAGGTTTCATTCCATATAAGATCTGTGCAAAACAAGATTGTGTATCAAGTTATGGGGAATGATACTGATATTATTAGTCCTTGGCTTATAAATGTTGTGAACTTCAGCTTAAAAAAGGAATCACTAGTACCTATTGTTTCTCAATTTGATGTATTTTATGATTATGATGGAGAAGAAGATGAAGAGCCACCAAAGCCATCACATGAAGTTGAAGGCTTGCGACGTTCGAAACGTAGGAATGTACAGCCTGAACGTTACGTAGGTTGTGTTGTTGAGAAGTTGGAAGTTGGTAATTTTCGAACGTGTCCCTACAAGAGAACAACTTATATGATAGAAGATGAAAACTCTTCTTCAGATTCAGAAGAGGATGGTGGTAAGAGGGATCAAAAGGTTAATAAGCTAAATCATTGCAAAGCTATTAATGTGAACACAAGGAACAAAAATTCTGACGGTGATGGCAAGTTTAAGGTACATAAAACTGATGACATTTCTTCCAAATATCATTATTTGACTAATTATATTTCTTGTGCAAAGAAGAATGGTGCTGATGTGTTGAATTTGGAGTACGGCAATGAGCCTACAACAATATCAAGTGATGAAGAAGTTGATGGTAATGTTACTTTGAGAAATTATAATAGTTTCTTTAAAGAGAAGATAAAGAGGAAGAGACTTCATGTTGTGGATGATATGGATTTTGAAACCAAATGGGAAGGGATACATTTCAAAAAAGAAGCTCCAACAGAAAATTTTCATTCGAGTTATTCGTCTAACAGAAGTCATGTTCATGAAGAACTAGATCATAAAGGCGGTAGAACATTCAATGCTGATGCCTGCAAGGAAATAATAGATAGTTACATGAAGAATTTTGATAGTCTACCAACTGAAGAAGAGCCTACTGTAAATGAGAAGTGGAAGGAAACAAGCATGTTGGAGAAAAAGGAAGAAGAAAAAGCATCGAAAAGCGATGACGAGGAAGAAAATCCTGATGATTTGGATGCCTTGTGGGAAGAAATGAATACAGCTTTGACATCAAGTTATCTTCTTGATGGAACTGCGGGTTCAACTGCAGAGTTTGTAGCTGATACCAAGAAAGAATCCAAGGATAGTTGTGAACATGATTATAGATTGGATGAAGAAATTGGAATATACTGTCTTCGATGTGGATTTGTGAAAACTGCAATACGAGACATTTCTGAGCCATTTGTGGAATGCCCAAAAAGGTACAAAGAGGAGAAACAGTGCAGTGAAGAAAATAAGGAACATAAATCAGAGCCAAAAGTTGATGAAGATTATAATAATGATATGTTCTCGACTCATGCTACTGATCCTGATGAACCATTGATTAATGAAATCGACAACGTTTGGGAATTGATTCCTGAACTTGAAGAAAAGATGCATGAACACCAGAAAAAAGCTTTTGAATTTCTTTGGCATAACATTGCTGGCTCTATGGAACCATCACTCATGGAAGAAAAATCCAACTCAAGTGGTTCAAGTTCGACGACAGCAAAATCAATGATTCTTCCTGGATTTCCAAGACCTTCTAGTGATGTCAAACATGTTTTGGATTGTCTTGGCAAGATACAGAAGTGGCACTCACACCCTAGTGTTCTTGTCATGGGCTATACTTCATTTTTACAATTAATGAGATCAAAAGATACAAAATTCGCACACAGAAAATACATGGCCAAAGCATTGAGAGAAAGCCCTGGAATCTTGATACTTGATGAAGGACACAATCCTAGAAGCACTAAATCAAGGTTGAGGAAATGTTTAATGAAACTTCCAACAGAATTGAGAATATTACTCTCCGGCACATTGTTTCAGAACAATTTTGGTGAATATTTCAACACACTTTGTTTAGCTAGACCGAAATTTATTCATGAAGTTCTACAAGAGTTAGATTCTAAATACAGAAGGAAAGGCGGAAAAGTTGCGAAGAAAGAACGTCATTTGCTTGAGGCACGCGCAAGAAAATTCTTCTTGAACAACATTGAAAAGAAAATTAACTCAGATATTGATGAGGAGAAGATGCAGGGTCTATATGTGCTGCGAAAAATCACTTCCGGTTTTATTGATGTGTACGACAGTGGAAATTCTTCTGAGACTCTTCCTGGTTTACAAATATATACATTGCTCATGAATACGTCCGATGAACAACATGAGATTGTACAGAAACTTCAGAAGAAAATGGCTGAGAGTACCAGTTACCCTCTTGAGGTTGAGCTTTTGATAACCCTCGGATCAATACATCCATGGTTGATCAAAACAGCTGCATCATGTGCAACTAAGTTTTTTGCGGAGGAAGAATTGAAGAAACTAGAAACAAGCAAATTTGATTTGAGAAAAGGTTCCAAAGTAAGATTTGTTCTGAGCCTAATCTCACGAGTAATGAAAAATGAAAAGGTTCTTATCTTTTGTCACAATCTTGCACCTGTGAGATTCTTAATAGAGTTATTTGAGAAATATTTTCAGTGGCAAAATGGCAAAGAGATTATGGTACTAACAGGTGAGCTAGACTTGTTTGAAAGAGGGAAAGTGATAGATAAATTTGAAGATCAAAGCAGCGGTTCAAAGATACTTCTTGCTTCAATCAATGCTTGTGCTGAAGGTATAAGTTTAACAGCAGCTTCTAGAGTGATTTTTCTGGACTCTGAGTGGAATCCTTCAAAAACAAAGCAAGCTATTGCACGCGCTTTTCGACCGGGCCAGCAGAAAATGGTTTATGTGTATCAGTTATTGACGACCGGGTCGATGGAAGAAGATAAGTATAGAAGAACTACTTGGAAAGAGTGGGTTTCTTGCATGATTTTTAGTGAGGAGTTTGTGGAAGATCCTTCTAAATGGCAAGCAGAGAAGATTGAAGATGATATACTTAGGGAGATGGTTGAAGAGGATAAGTCTAAAGCTATTCACATGATTATGAAGAATGAAAAGGCTTCAACAATCTAA |
| Protein sequence |
>MsG0380015525.01.T01 MAPIKRHLHQWKHPFDANYLASPNSCFKTAFEAVLNGSWQPVELLKIESGKTTLRFIDTEQIQSTLSSDIRIRSRKATSSDCSSFLRPGIDISMLLGYRYNDNSDPFTSIPVWIDARINSIHRQPHKSDQCSCQFYVNLYDDQGSLGTEVKTLNKEVNAIEIDQIFILQRLQHNTCEGLLKENDTSEIKPYRWDSSEDCSSLSQSRLLVGKFSSDLSWFVITSFLKKVSFHIRSVQNKIVYQVMGNDTDIISPWLINVVNFSLKKESLVPIVSQFDVFYDYDGEEDEEPPKPSHEVEGLRRSKRRNVQPERYVGCVVEKLEVGNFRTCPYKRTTYMIEDENSSSDSEEDGGKRDQKVNKLNHCKAINVNTRNKNSDGDGKFKVHKTDDISSKYHYLTNYISCAKKNGADVLNLEYGNEPTTISSDEEVDGNVTLRNYNSFFKEKIKRKRLHVVDDMDFETKWEGIHFKKEAPTENFHSSYSSNRSHVHEELDHKGGRTFNADACKEIIDSYMKNFDSLPTEEEPTVNEKWKETSMLEKKEEEKASKSDDEEENPDDLDALWEEMNTALTSSYLLDGTAGSTAEFVADTKKESKDSCEHDYRLDEEIGIYCLRCGFVKTAIRDISEPFVECPKRYKEEKQCSEENKEHKSEPKVDEDYNNDMFSTHATDPDEPLINEIDNVWELIPELEEKMHEHQKKAFEFLWHNIAGSMEPSLMEEKSNSSGSSSTTAKSMILPGFPRPSSDVKHVLDCLGKIQKWHSHPSVLVMGYTSFLQLMRSKDTKFAHRKYMAKALRESPGILILDEGHNPRSTKSRLRKCLMKLPTELRILLSGTLFQNNFGEYFNTLCLARPKFIHEVLQELDSKYRRKGGKVAKKERHLLEARARKFFLNNIEKKINSDIDEEKMQGLYVLRKITSGFIDVYDSGNSSETLPGLQIYTLLMNTSDEQHEIVQKLQKKMAESTSYPLEVELLITLGSIHPWLIKTAASCATKFFAEEELKKLETSKFDLRKGSKVRFVLSLISRVMKNEKVLIFCHNLAPVRFLIELFEKYFQWQNGKEIMVLTGELDLFERGKVIDKFEDQSSGSKILLASINACAEGISLTAASRVIFLDSEWNPSKTKQAIARAFRPGQQKMVYVYQLLTTGSMEEDKYRRTTWKEWVSCMIFSEEFVEDPSKWQAEKIEDDILREMVEEDKSKAIHMIMKNEKASTI* |
