AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380015775.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380015775.01.T02 MTR_3g077240 100.000 170 0 0 1 170 22 191 8.31e-125 349
MsG0380015775.01.T02 MTR_4g105170 72.671 161 34 4 1 156 23 178 4.53e-73 218
MsG0380015775.01.T02 MTR_7g033800 55.233 172 55 2 1 172 23 172 1.12e-56 176
MsG0380015775.01.T02 MTR_6g005070 82.418 91 16 0 4 94 24 114 2.58e-53 166
MsG0380015775.01.T02 MTR_6g005080 81.319 91 17 0 4 94 25 115 8.46e-52 162
MsG0380015775.01.T02 MTR_5g083230 72.414 87 24 0 4 90 21 107 6.09e-40 133
MsG0380015775.01.T02 MTR_8g040900 73.626 91 24 0 2 92 20 110 1.43e-39 134
MsG0380015775.01.T02 MTR_2g068760 54.545 121 55 0 4 124 28 148 2.99e-39 131
MsG0380015775.01.T02 MTR_3g073690 52.941 136 60 2 4 136 24 158 1.37e-38 130
MsG0380015775.01.T02 MTR_5g017950 51.515 132 54 1 4 135 23 144 2.70e-38 133
MsG0380015775.01.T02 MTR_5g083010 51.587 126 50 1 1 115 23 148 4.52e-38 129
MsG0380015775.01.T02 MTR_4g083680 65.169 89 31 0 4 92 30 118 1.94e-37 127
MsG0380015775.01.T02 MTR_3g031660 65.517 87 30 0 4 90 22 108 2.86e-37 127
MsG0380015775.01.T02 MTR_3g071590 67.816 87 28 0 4 90 23 109 1.09e-36 124
MsG0380015775.01.T02 MTR_3g452660 50.806 124 61 0 4 127 22 145 1.65e-34 122
MsG0380015775.01.T02 MTR_5g083960 50.400 125 53 2 4 119 54 178 1.76e-34 120
MsG0380015775.01.T02 MTR_3g094690 60.215 93 37 0 4 96 62 154 1.86e-34 121
MsG0380015775.01.T02 MTR_3g071420 66.667 81 27 0 2 82 27 107 1.91e-34 119
MsG0380015775.01.T02 MTR_2g093310 55.556 90 40 0 3 92 47 136 5.40e-33 116
MsG0380015775.01.T02 MTR_3g094690 59.140 93 37 1 4 96 62 153 6.04e-32 115
MsG0380015775.01.T02 MTR_4g060950 53.763 93 43 0 4 96 40 132 6.42e-32 113
MsG0380015775.01.T02 MTR_1g095850 58.621 87 36 0 4 90 87 173 3.34e-31 112
MsG0380015775.01.T02 MTR_5g075020 57.018 114 39 2 4 107 58 171 6.24e-30 109
MsG0380015775.01.T02 MTR_5g075020 57.018 114 39 2 4 107 62 175 9.53e-30 109
MsG0380015775.01.T02 MTR_7g028905 48.958 96 46 1 2 97 18 110 5.35e-28 101
MsG0380015775.01.T02 MTR_7g096530 55.056 89 39 1 2 89 27 115 9.93e-28 103
MsG0380015775.01.T02 MTR_8g036085 52.688 93 43 1 1 92 35 127 2.21e-27 103
MsG0380015775.01.T02 MTR_8g036105 52.381 105 49 1 1 104 30 134 6.50e-27 101
MsG0380015775.01.T02 MTR_1g070220 56.977 86 36 1 4 88 30 115 5.20e-26 99.0
MsG0380015775.01.T02 MTR_7g074990 58.025 81 33 1 3 82 34 114 7.49e-25 96.7
MsG0380015775.01.T02 MTR_8g079660 44.681 94 52 0 4 97 23 116 1.61e-24 96.3
MsG0380015775.01.T02 MTR_5g093010 48.837 86 44 0 4 89 20 105 2.37e-24 92.8
MsG0380015775.01.T02 MTR_6g027710 47.475 99 49 2 3 98 54 152 1.04e-23 93.2
MsG0380015775.01.T02 MTR_5g080470 50.617 81 39 1 4 83 22 102 8.46e-23 90.1
MsG0380015775.01.T02 MTR_7g075230 53.409 88 39 1 4 89 28 115 2.32e-22 89.0
MsG0380015775.01.T02 MTR_7g096610 53.086 81 37 1 3 82 22 102 6.10e-22 88.6
MsG0380015775.01.T02 MTR_8g079620 43.820 89 50 0 4 92 23 111 7.27e-22 89.0
MsG0380015775.01.T02 MTR_8g079580 44.211 95 53 0 3 97 23 117 8.07e-22 88.6
MsG0380015775.01.T02 MTR_1g070205 49.398 83 41 1 1 82 24 106 1.39e-21 87.8
MsG0380015775.01.T02 MTR_4g107450 45.679 81 44 0 2 82 23 103 1.59e-20 86.3
MsG0380015775.01.T02 MTR_6g027700 44.706 85 46 1 4 87 22 106 1.43e-19 82.0
MsG0380015775.01.T02 MTR_6g011200 43.210 81 45 1 4 83 24 104 2.43e-19 82.4
MsG0380015775.01.T02 MTR_8g017090 39.560 91 55 0 2 92 25 115 4.65e-18 79.7
MsG0380015775.01.T02 MTR_6g011230 32.258 124 81 1 4 124 26 149 3.51e-17 75.5
MsG0380015775.01.T02 MTR_6g011250 36.364 110 65 2 4 108 24 133 4.91e-16 72.4
MsG0380015775.01.T02 MTR_2g100020 38.750 80 49 0 3 82 33 112 1.54e-15 72.4
MsG0380015775.01.T02 MTR_4g099230 36.145 83 52 1 2 83 15 97 2.38e-15 69.7
MsG0380015775.01.T02 MTR_4g088035 29.139 151 90 4 1 142 18 160 1.21e-12 64.3
MsG0380015775.01.T02 MTR_5g085390 35.632 87 55 1 4 89 24 110 7.44e-12 62.0
MsG0380015775.01.T01 MTR_3g077240 100.000 191 0 0 1 191 1 191 1.26e-141 392
MsG0380015775.01.T01 MTR_4g105170 75.000 176 34 4 7 177 8 178 4.64e-86 252
MsG0380015775.01.T01 MTR_7g033800 59.278 194 56 3 1 193 1 172 2.21e-71 214
MsG0380015775.01.T01 MTR_6g005070 64.151 159 30 2 1 159 1 132 1.33e-65 198
MsG0380015775.01.T01 MTR_6g005080 78.261 115 25 0 1 115 1 115 5.10e-64 194
MsG0380015775.01.T01 MTR_8g040900 75.221 113 25 1 1 113 1 110 3.99e-52 167
MsG0380015775.01.T01 MTR_5g017950 55.556 153 58 1 4 156 2 144 2.37e-51 167
MsG0380015775.01.T01 MTR_5g083230 71.154 104 30 0 8 111 4 107 3.59e-50 160
MsG0380015775.01.T01 MTR_2g068760 56.204 137 60 0 9 145 12 148 3.92e-49 157
MsG0380015775.01.T01 MTR_3g073690 54.140 157 68 2 4 157 3 158 3.80e-48 156
MsG0380015775.01.T01 MTR_4g083680 65.714 105 36 0 9 113 14 118 2.86e-47 153
MsG0380015775.01.T01 MTR_3g452660 55.245 143 64 0 5 147 2 144 8.42e-47 155
MsG0380015775.01.T01 MTR_5g083010 53.571 140 54 1 8 136 9 148 9.41e-47 152
MsG0380015775.01.T01 MTR_3g031660 64.423 104 37 0 8 111 5 108 2.70e-46 150
MsG0380015775.01.T01 MTR_3g071590 66.346 104 35 0 8 111 6 109 8.64e-46 149
MsG0380015775.01.T01 MTR_5g083960 51.408 142 60 2 8 140 37 178 8.43e-44 145
MsG0380015775.01.T01 MTR_3g094690 60.550 109 43 0 9 117 46 154 2.06e-43 145
MsG0380015775.01.T01 MTR_3g071420 65.625 96 33 0 8 103 12 107 2.37e-42 140
MsG0380015775.01.T01 MTR_2g093310 55.963 109 48 0 9 117 32 140 2.38e-42 141
MsG0380015775.01.T01 MTR_3g094690 59.633 109 43 1 9 117 46 153 6.99e-41 138
MsG0380015775.01.T01 MTR_4g060950 55.455 110 48 1 9 117 23 132 1.11e-39 134
MsG0380015775.01.T01 MTR_1g095850 58.252 103 43 0 11 113 73 175 4.43e-39 134
MsG0380015775.01.T01 MTR_5g075020 57.692 130 45 2 9 128 42 171 5.02e-39 134
MsG0380015775.01.T01 MTR_5g075020 57.692 130 45 2 9 128 46 175 9.87e-39 133
MsG0380015775.01.T01 MTR_7g096530 57.798 109 45 1 3 110 7 115 2.98e-38 131
MsG0380015775.01.T01 MTR_8g036085 57.143 105 44 1 10 113 23 127 2.64e-37 129
MsG0380015775.01.T01 MTR_8g036105 56.410 117 50 1 10 125 18 134 1.07e-36 127
MsG0380015775.01.T01 MTR_1g070220 54.701 117 47 2 1 111 1 117 8.47e-36 124
MsG0380015775.01.T01 MTR_7g074990 61.053 95 36 1 10 103 20 114 9.73e-35 122
MsG0380015775.01.T01 MTR_7g028905 50.909 110 51 1 9 118 4 110 1.95e-34 118
MsG0380015775.01.T01 MTR_6g027710 51.818 110 50 2 9 115 39 148 2.20e-33 119
MsG0380015775.01.T01 MTR_8g079660 46.789 109 58 0 10 118 8 116 3.86e-32 117
MsG0380015775.01.T01 MTR_5g080470 51.485 101 48 1 5 104 2 102 3.90e-32 115
MsG0380015775.01.T01 MTR_7g096610 57.292 96 40 1 9 103 7 102 6.12e-32 115
MsG0380015775.01.T01 MTR_1g070205 54.737 95 42 1 10 103 12 106 1.58e-31 114
MsG0380015775.01.T01 MTR_7g075230 56.731 104 42 2 10 110 12 115 3.15e-31 112
MsG0380015775.01.T01 MTR_5g093010 44.628 121 65 1 11 129 6 126 1.87e-30 109
MsG0380015775.01.T01 MTR_8g079620 47.115 104 55 0 10 113 8 111 7.95e-30 110
MsG0380015775.01.T01 MTR_4g107450 48.000 100 52 0 11 110 11 110 4.52e-29 109
MsG0380015775.01.T01 MTR_8g079580 46.364 110 58 1 10 118 8 117 5.33e-28 105
MsG0380015775.01.T01 MTR_6g011200 45.918 98 52 1 8 104 7 104 9.42e-28 105
MsG0380015775.01.T01 MTR_6g027700 47.000 100 52 1 10 108 7 106 2.95e-27 102
MsG0380015775.01.T01 MTR_8g017090 43.689 103 58 0 11 113 13 115 4.16e-26 102
MsG0380015775.01.T01 MTR_6g011230 35.252 139 85 2 11 145 12 149 4.09e-25 97.1
MsG0380015775.01.T01 MTR_6g011250 39.370 127 72 2 8 129 7 133 6.47e-24 93.6
MsG0380015775.01.T01 MTR_4g099230 41.053 95 55 1 11 104 3 97 3.26e-23 90.9
MsG0380015775.01.T01 MTR_2g100020 43.011 93 53 0 11 103 20 112 3.46e-23 93.6
MsG0380015775.01.T01 MTR_5g085390 39.423 104 62 1 8 110 7 110 7.91e-20 84.0
MsG0380015775.01.T01 MTR_4g088035 29.878 164 98 4 9 163 5 160 3.16e-18 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380015775.01.T02 AT5G63090 71.429 126 19 1 1 109 23 148 3.73e-58 180
MsG0380015775.01.T02 AT5G63090 71.429 126 19 1 1 109 23 148 3.73e-58 180
MsG0380015775.01.T02 AT5G63090 71.429 126 19 1 1 109 23 148 3.73e-58 180
MsG0380015775.01.T02 AT5G63090 71.429 126 19 1 1 109 23 148 3.73e-58 180
MsG0380015775.01.T02 AT3G27650 81.111 90 17 0 4 93 54 143 1.31e-51 162
MsG0380015775.01.T02 AT2G30130 71.264 87 25 0 4 90 23 109 1.22e-39 133
MsG0380015775.01.T02 AT2G23660 59.804 102 37 1 4 105 20 117 5.87e-39 135
MsG0380015775.01.T02 AT2G23660 59.804 102 37 1 4 105 20 117 5.87e-39 135
MsG0380015775.01.T02 AT2G23660 59.804 102 37 1 4 105 20 117 5.87e-39 135
MsG0380015775.01.T02 AT5G66870 60.000 105 38 1 4 108 22 122 1.46e-38 134
MsG0380015775.01.T02 AT1G65620 70.330 91 27 0 2 92 22 112 9.57e-38 129
MsG0380015775.01.T02 AT1G65620 70.330 91 27 0 2 92 22 112 9.57e-38 129
MsG0380015775.01.T02 AT1G65620 70.330 91 27 0 2 92 22 112 9.57e-38 129
MsG0380015775.01.T02 AT1G65620 70.330 91 27 0 2 92 22 112 9.57e-38 129
MsG0380015775.01.T02 AT1G65620 70.330 91 27 0 2 92 22 112 9.57e-38 129
MsG0380015775.01.T02 AT2G30340 57.000 100 43 0 4 103 67 166 8.43e-35 123
MsG0380015775.01.T02 AT2G30340 57.000 100 43 0 4 103 68 167 8.90e-35 123
MsG0380015775.01.T02 AT1G16530 61.111 90 35 0 3 92 29 118 1.16e-34 119
MsG0380015775.01.T02 AT1G31320 73.611 72 19 0 4 75 28 99 3.15e-34 119
MsG0380015775.01.T02 AT1G07900 57.143 91 39 0 4 94 48 138 9.55e-34 118
MsG0380015775.01.T02 AT2G28500 58.621 87 36 0 4 90 70 156 1.12e-31 114
MsG0380015775.01.T02 AT3G11090 53.333 105 46 2 2 106 24 125 4.12e-31 110
MsG0380015775.01.T02 AT3G26660 54.651 86 39 0 4 89 20 105 7.70e-31 108
MsG0380015775.01.T02 AT3G26620 54.651 86 39 0 4 89 20 105 8.40e-31 108
MsG0380015775.01.T02 AT2G40470 52.500 120 44 2 4 118 35 146 2.72e-29 107
MsG0380015775.01.T02 AT2G40470 52.500 120 44 2 4 118 60 171 5.09e-29 107
MsG0380015775.01.T02 AT2G45420 55.556 90 39 1 1 89 49 138 1.78e-27 103
MsG0380015775.01.T02 AT4G00220 56.818 88 37 1 3 89 31 118 2.43e-27 102
MsG0380015775.01.T02 AT5G35900 47.170 106 56 0 2 107 18 123 3.94e-26 99.4
MsG0380015775.01.T02 AT2G45410 56.818 88 37 1 3 89 30 117 1.26e-25 97.4
MsG0380015775.01.T02 AT3G03760 50.549 91 44 1 1 90 63 153 2.19e-25 99.0
MsG0380015775.01.T02 AT5G06080 39.855 138 79 3 4 140 22 156 4.74e-24 93.2
MsG0380015775.01.T02 AT2G42430 51.724 87 41 1 4 89 30 116 1.50e-23 93.6
MsG0380015775.01.T02 AT4G00210 48.544 103 44 2 4 105 26 120 3.80e-23 91.7
MsG0380015775.01.T02 AT2G31310 50.602 83 40 1 1 82 19 101 3.91e-23 90.9
MsG0380015775.01.T02 AT3G58190 51.220 82 39 1 3 83 25 106 1.45e-22 90.1
MsG0380015775.01.T02 AT2G42440 47.619 84 43 1 1 83 19 102 1.15e-21 88.6
MsG0380015775.01.T02 AT3G50510 41.176 102 57 1 4 105 27 125 1.64e-20 84.3
MsG0380015775.01.T02 AT3G50510 41.176 102 57 1 4 105 27 125 1.64e-20 84.3
MsG0380015775.01.T02 AT3G13850 40.659 91 54 0 2 92 49 139 1.78e-20 85.9
MsG0380015775.01.T02 AT3G47870 39.535 86 52 0 4 89 51 136 2.65e-17 77.8
MsG0380015775.01.T02 AT4G22700 36.752 117 70 3 2 115 18 133 7.15e-16 72.0
MsG0380015775.01.T02 AT1G72980 42.683 82 47 0 2 83 26 107 7.67e-16 72.4
MsG0380015775.01.T02 AT1G06280 42.857 63 36 0 4 66 39 101 3.09e-12 62.4
MsG0380015775.01.T02 AT2G19820 37.838 74 45 1 4 76 27 100 8.38e-11 57.0
MsG0380015775.01.T01 AT5G63090 75.000 148 19 2 1 130 1 148 3.55e-73 219
MsG0380015775.01.T01 AT5G63090 75.000 148 19 2 1 130 1 148 3.55e-73 219
MsG0380015775.01.T01 AT5G63090 75.000 148 19 2 1 130 1 148 3.55e-73 219
MsG0380015775.01.T01 AT5G63090 75.000 148 19 2 1 130 1 148 3.55e-73 219
MsG0380015775.01.T01 AT3G27650 79.279 111 23 0 4 114 33 143 4.19e-63 192
MsG0380015775.01.T01 AT1G65620 73.451 113 29 1 1 113 1 112 3.94e-51 164
MsG0380015775.01.T01 AT1G65620 73.451 113 29 1 1 113 1 112 3.94e-51 164
MsG0380015775.01.T01 AT1G65620 73.451 113 29 1 1 113 1 112 3.94e-51 164
MsG0380015775.01.T01 AT1G65620 73.451 113 29 1 1 113 1 112 3.94e-51 164
MsG0380015775.01.T01 AT1G65620 73.451 113 29 1 1 113 1 112 3.94e-51 164
MsG0380015775.01.T01 AT5G66870 62.791 129 41 2 1 129 1 122 2.17e-50 165
MsG0380015775.01.T01 AT2G23660 60.504 119 43 1 8 126 3 117 7.36e-49 161
MsG0380015775.01.T01 AT2G23660 60.504 119 43 1 8 126 3 117 7.36e-49 161
MsG0380015775.01.T01 AT2G23660 60.504 119 43 1 8 126 3 117 7.36e-49 161
MsG0380015775.01.T01 AT2G30130 69.231 104 32 0 8 111 6 109 8.44e-49 157
MsG0380015775.01.T01 AT2G30340 57.759 116 49 0 9 124 52 167 4.48e-44 147
MsG0380015775.01.T01 AT2G30340 57.759 116 49 0 9 124 51 166 4.50e-44 147
MsG0380015775.01.T01 AT1G31320 72.727 88 24 0 9 96 12 99 1.42e-43 143
MsG0380015775.01.T01 AT1G07900 58.333 108 45 0 9 116 32 139 2.42e-43 143
MsG0380015775.01.T01 AT1G16530 62.264 106 39 1 9 113 13 118 1.36e-42 140
MsG0380015775.01.T01 AT2G28500 60.194 103 41 0 9 111 54 156 2.46e-41 139
MsG0380015775.01.T01 AT3G11090 54.167 120 52 2 8 127 9 125 1.58e-39 133
MsG0380015775.01.T01 AT3G26660 56.000 100 44 0 11 110 6 105 2.83e-38 128
MsG0380015775.01.T01 AT3G26620 56.000 100 44 0 11 110 6 105 3.05e-38 128
MsG0380015775.01.T01 AT2G40470 53.676 136 50 2 9 139 19 146 3.72e-38 130
MsG0380015775.01.T01 AT2G40470 53.676 136 50 2 9 139 44 171 7.47e-38 130
MsG0380015775.01.T01 AT3G03760 55.769 104 45 1 9 111 50 153 3.60e-36 127
MsG0380015775.01.T01 AT2G45420 59.596 99 39 1 13 110 40 138 2.96e-35 125
MsG0380015775.01.T01 AT4G00220 59.596 99 39 1 13 110 20 118 3.79e-35 124
MsG0380015775.01.T01 AT5G06080 42.405 158 87 3 5 161 2 156 6.12e-34 119
MsG0380015775.01.T01 AT2G42430 55.340 103 45 1 9 110 14 116 1.09e-33 120
MsG0380015775.01.T01 AT2G45410 55.085 118 47 3 13 126 19 134 2.66e-33 117
MsG0380015775.01.T01 AT4G00210 49.600 125 54 2 3 126 4 120 2.67e-33 119
MsG0380015775.01.T01 AT2G31310 55.208 96 42 1 9 103 6 101 1.19e-32 116
MsG0380015775.01.T01 AT3G58190 55.208 96 42 1 10 104 11 106 2.00e-32 116
MsG0380015775.01.T01 AT2G42440 52.577 97 45 1 9 104 6 102 8.28e-32 115
MsG0380015775.01.T01 AT5G35900 46.610 118 63 0 11 128 6 123 3.28e-31 113
MsG0380015775.01.T01 AT3G50510 44.915 118 62 1 9 126 11 125 4.70e-30 109
MsG0380015775.01.T01 AT3G50510 44.915 118 62 1 9 126 11 125 4.70e-30 109
MsG0380015775.01.T01 AT3G13850 44.660 103 57 0 11 113 37 139 1.55e-28 107
MsG0380015775.01.T01 AT3G47870 42.000 100 58 0 11 110 37 136 9.27e-25 99.0
MsG0380015775.01.T01 AT1G72980 44.792 96 53 0 9 104 12 107 8.74e-23 91.3
MsG0380015775.01.T01 AT4G22700 37.984 129 76 3 11 136 6 133 2.42e-21 87.0
MsG0380015775.01.T01 AT1G06280 42.500 80 46 0 8 87 22 101 5.44e-18 78.6
MsG0380015775.01.T01 AT5G15060 40.260 77 44 1 7 81 14 90 5.53e-15 68.9
MsG0380015775.01.T01 AT2G19820 36.667 90 56 1 9 97 11 100 5.92e-15 68.6
MsG0380015775.01.T01 AT3G27940 39.394 66 40 0 10 75 5 70 2.45e-11 59.7
MsG0380015775.01.T01 AT3G27940 39.394 66 40 0 10 75 5 70 2.45e-11 59.7
MsG0380015775.01.T01 AT3G27940 39.394 66 40 0 10 75 5 70 2.45e-11 59.7

Find 49 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGGTGGAAGAGAGGAATT+AGG 0.310902 3:-74891013 None:intergenic
AGACAGTTTAGTTCAAGATT+TGG 0.321989 3:+74891076 MsG0380015775.01.T01:CDS
TCTCCCTCCTCTTGTTGTTC+CGG 0.367027 3:+74891033 MsG0380015775.01.T01:CDS
GGTTCTTCTGGTGGAAAGTA+TGG 0.385993 3:-74890740 None:intergenic
TTTGGTAATGGAAATGTTGA+TGG 0.387117 3:+74891094 MsG0380015775.01.T01:CDS
AATGGAAATGTTGATGGAAA+TGG 0.392662 3:+74891100 MsG0380015775.01.T01:CDS
GGAGGAAGAGGAGGAGGTGT+AGG 0.401282 3:+74891196 MsG0380015775.01.T01:CDS
GGTGTAGGAGGAAGAGGAGG+AGG 0.413675 3:+74891190 MsG0380015775.01.T01:CDS
GGAAGAGGAGGAGGTGTAGG+AGG 0.430574 3:+74891199 MsG0380015775.01.T01:CDS
AGTGGAGGTGTAGGAGGAAG+AGG 0.435749 3:+74891184 MsG0380015775.01.T01:CDS
GATATAAGTGGAGGTGTAGG+AGG 0.445011 3:+74891178 MsG0380015775.01.T01:CDS
AGGAGGGAGAGGTGGAAGAG+AGG 0.445135 3:-74891020 None:intergenic
AGAGGAGGAGGTGTAGGAGG+AGG 0.445819 3:+74891202 MsG0380015775.01.T01:CDS
TTTAGTTCAAGATTTGGTAA+TGG 0.446442 3:+74891082 MsG0380015775.01.T01:CDS
CGTGTGAGAGATCCTGTTTA+TGG 0.449251 3:+74890884 MsG0380015775.01.T01:CDS
AATTCACTGCATCTTCTCTC+TGG 0.451599 3:-74890838 None:intergenic
TTAATCCATGGAATAGCATA+AGG 0.469520 3:-74891139 None:intergenic
GCACCAACACAACCATAAAC+AGG 0.472967 3:-74890896 None:intergenic
GAGGATATAAGTGGAGGTGT+AGG 0.488241 3:+74891175 MsG0380015775.01.T01:CDS
GCCAACGTTCACAAGATCTT+CGG 0.491748 3:+74890773 MsG0380015775.01.T01:CDS
CTGCATCTTCTCTCTGGTGA+GGG 0.502538 3:-74890832 None:intergenic
CTTCGTCGAAAATGCATGCC+AGG 0.503647 3:+74890704 MsG0380015775.01.T01:CDS
ACAAGTTCAGAGGCTTCAAA+AGG 0.506762 3:+74890937 MsG0380015775.01.T01:CDS
GCCTCTGAACTTGTCTCTGA+AGG 0.518925 3:-74890928 None:intergenic
GATCCTGTTTATGGTTGTGT+TGG 0.519160 3:+74890893 MsG0380015775.01.T01:CDS
GGAGGTGTAGGAGGAAGAGG+AGG 0.524745 3:+74891187 MsG0380015775.01.T01:CDS
ACCTCTTCTGAGGATATAAG+TGG 0.541161 3:+74891166 MsG0380015775.01.T01:CDS
ACAACAAGAGGAGGGAGAGG+TGG 0.543814 3:-74891028 None:intergenic
CATCTTCTCTCTGGTGAGGG+AGG 0.545055 3:-74890829 None:intergenic
AGGAATTTGCAGGCTGCACA+TGG 0.547297 3:-74890683 None:intergenic
ACTGCATCTTCTCTCTGGTG+AGG 0.556115 3:-74890833 None:intergenic
TGAGATTGATGAAACGAAGC+CGG 0.556305 3:-74891052 None:intergenic
GGAACAACAAGAGGAGGGAG+AGG 0.557580 3:-74891031 None:intergenic
TCCACTTATATCCTCAGAAG+AGG 0.570244 3:-74891167 None:intergenic
TCCTTCAGAGACAAGTTCAG+AGG 0.577279 3:+74890927 MsG0380015775.01.T01:CDS
GATTAATGATACCTCTTCTG+AGG 0.582743 3:+74891156 MsG0380015775.01.T01:CDS
AACGAAGCCGGAACAACAAG+AGG 0.585009 3:-74891040 None:intergenic
TATGGTGCAAAGATGCAGCC+TGG 0.588900 3:-74890722 None:intergenic
TTCCCTTGCTTATGAAGCCG+AGG 0.588970 3:+74890859 MsG0380015775.01.T01:CDS
AGGATCTCTCACACGTGCCT+CGG 0.589439 3:-74890876 None:intergenic
GAAGAGGTATCATTAATCCA+TGG 0.590052 3:-74891151 None:intergenic
GTGCCTCGGCTTCATAAGCA+AGG 0.591344 3:-74890862 None:intergenic
GAAGCCGGAACAACAAGAGG+AGG 0.593525 3:-74891037 None:intergenic
GCCGAAGATCTTGTGAACGT+TGG 0.617944 3:-74890774 None:intergenic
TTGTCTCTGAAGGAAAGAGA+TGG 0.626573 3:-74890918 None:intergenic
AAGCCGGAACAACAAGAGGA+GGG 0.639232 3:-74891036 None:intergenic
TGCCTCGGCTTCATAAGCAA+GGG 0.648971 3:-74890861 None:intergenic
GGTGAGGGAGGAGTTCGTTG+AGG 0.665155 3:-74890817 None:intergenic
TCTTCTGAGGATATAAGTGG+AGG 0.670110 3:+74891169 MsG0380015775.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAGTTCAAGATTTGGTAA+TGG + Chr3:74891082-74891101 MsG0380015775.01.T01:CDS 25.0%
TTAATCCATGGAATAGCATA+AGG - Chr3:74891142-74891161 None:intergenic 30.0%
! AATGGAAATGTTGATGGAAA+TGG + Chr3:74891100-74891119 MsG0380015775.01.T01:CDS 30.0%
! AGACAGTTTAGTTCAAGATT+TGG + Chr3:74891076-74891095 MsG0380015775.01.T01:CDS 30.0%
!! TTTGGTAATGGAAATGTTGA+TGG + Chr3:74891094-74891113 MsG0380015775.01.T01:CDS 30.0%
GATTAATGATACCTCTTCTG+AGG + Chr3:74891156-74891175 MsG0380015775.01.T01:CDS 35.0%
! CTTTTCCTTATGCTATTCCA+TGG + Chr3:74891134-74891153 MsG0380015775.01.T01:CDS 35.0%
!! GAAGAGGTATCATTAATCCA+TGG - Chr3:74891154-74891173 None:intergenic 35.0%
!!! AATTTTTGAGGTTCTTCTGG+TGG - Chr3:74890752-74890771 None:intergenic 35.0%
AATTCACTGCATCTTCTCTC+TGG - Chr3:74890841-74890860 None:intergenic 40.0%
ACAAGTTCAGAGGCTTCAAA+AGG + Chr3:74890937-74890956 MsG0380015775.01.T01:CDS 40.0%
GATCCTGTTTATGGTTGTGT+TGG + Chr3:74890893-74890912 MsG0380015775.01.T01:CDS 40.0%
TCCACTTATATCCTCAGAAG+AGG - Chr3:74891170-74891189 None:intergenic 40.0%
TGAGATTGATGAAACGAAGC+CGG - Chr3:74891055-74891074 None:intergenic 40.0%
! ACCTCTTCTGAGGATATAAG+TGG + Chr3:74891166-74891185 MsG0380015775.01.T01:CDS 40.0%
! TCTTCTGAGGATATAAGTGG+AGG + Chr3:74891169-74891188 MsG0380015775.01.T01:CDS 40.0%
! TGAACGTTGGCGAATTTTTG+AGG - Chr3:74890764-74890783 None:intergenic 40.0%
! TTGTCTCTGAAGGAAAGAGA+TGG - Chr3:74890921-74890940 None:intergenic 40.0%
! TTTTCGACGAAGGAATTTGC+AGG - Chr3:74890696-74890715 None:intergenic 40.0%
!!! GCGAATTTTTGAGGTTCTTC+TGG - Chr3:74890755-74890774 None:intergenic 40.0%
AGAGGTGGAAGAGAGGAATT+AGG - Chr3:74891016-74891035 None:intergenic 45.0%
CGTGTGAGAGATCCTGTTTA+TGG + Chr3:74890884-74890903 MsG0380015775.01.T01:CDS 45.0%
GCACCAACACAACCATAAAC+AGG - Chr3:74890899-74890918 None:intergenic 45.0%
GCCAACGTTCACAAGATCTT+CGG + Chr3:74890773-74890792 MsG0380015775.01.T01:CDS 45.0%
GGTTCTTCTGGTGGAAAGTA+TGG - Chr3:74890743-74890762 None:intergenic 45.0%
TCCTTCAGAGACAAGTTCAG+AGG + Chr3:74890927-74890946 MsG0380015775.01.T01:CDS 45.0%
! GAGGATATAAGTGGAGGTGT+AGG + Chr3:74891175-74891194 MsG0380015775.01.T01:CDS 45.0%
! GATATAAGTGGAGGTGTAGG+AGG + Chr3:74891178-74891197 MsG0380015775.01.T01:CDS 45.0%
AACGAAGCCGGAACAACAAG+AGG - Chr3:74891043-74891062 None:intergenic 50.0%
AAGCCGGAACAACAAGAGGA+GGG - Chr3:74891039-74891058 None:intergenic 50.0%
ACTGCATCTTCTCTCTGGTG+AGG - Chr3:74890836-74890855 None:intergenic 50.0%
AGGAATTTGCAGGCTGCACA+TGG - Chr3:74890686-74890705 None:intergenic 50.0%
CTGCATCTTCTCTCTGGTGA+GGG - Chr3:74890835-74890854 None:intergenic 50.0%
CTTCGTCGAAAATGCATGCC+AGG + Chr3:74890704-74890723 MsG0380015775.01.T01:CDS 50.0%
GCCGAAGATCTTGTGAACGT+TGG - Chr3:74890777-74890796 None:intergenic 50.0%
GCCTCTGAACTTGTCTCTGA+AGG - Chr3:74890931-74890950 None:intergenic 50.0%
TATGGTGCAAAGATGCAGCC+TGG - Chr3:74890725-74890744 None:intergenic 50.0%
TGCCTCGGCTTCATAAGCAA+GGG - Chr3:74890864-74890883 None:intergenic 50.0%
TTCCCTTGCTTATGAAGCCG+AGG + Chr3:74890859-74890878 MsG0380015775.01.T01:CDS 50.0%
! CTGGCATGCATTTTCGACGA+AGG - Chr3:74890706-74890725 None:intergenic 50.0%
!! TCTCCCTCCTCTTGTTGTTC+CGG + Chr3:74891033-74891052 MsG0380015775.01.T01:CDS 50.0%
ACAACAAGAGGAGGGAGAGG+TGG - Chr3:74891031-74891050 None:intergenic 55.0%
AGGATCTCTCACACGTGCCT+CGG - Chr3:74890879-74890898 None:intergenic 55.0%
CATCTTCTCTCTGGTGAGGG+AGG - Chr3:74890832-74890851 None:intergenic 55.0%
GAAGCCGGAACAACAAGAGG+AGG - Chr3:74891040-74891059 None:intergenic 55.0%
GGAACAACAAGAGGAGGGAG+AGG - Chr3:74891034-74891053 None:intergenic 55.0%
GTGCCTCGGCTTCATAAGCA+AGG - Chr3:74890865-74890884 None:intergenic 55.0%
! AGTGGAGGTGTAGGAGGAAG+AGG + Chr3:74891184-74891203 MsG0380015775.01.T01:CDS 55.0%
AGGAGGGAGAGGTGGAAGAG+AGG - Chr3:74891023-74891042 None:intergenic 60.0%
GGTGAGGGAGGAGTTCGTTG+AGG - Chr3:74890820-74890839 None:intergenic 60.0%
GGTGTAGGAGGAAGAGGAGG+AGG + Chr3:74891190-74891209 MsG0380015775.01.T01:CDS 60.0%
! AGAGGAGGAGGTGTAGGAGG+AGG + Chr3:74891202-74891221 MsG0380015775.01.T01:CDS 60.0%
! GGAAGAGGAGGAGGTGTAGG+AGG + Chr3:74891199-74891218 MsG0380015775.01.T01:CDS 60.0%
! GGAGGAAGAGGAGGAGGTGT+AGG + Chr3:74891196-74891215 MsG0380015775.01.T01:CDS 60.0%
! GGAGGTGTAGGAGGAAGAGG+AGG + Chr3:74891187-74891206 MsG0380015775.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 74890656 74891237 74890656 ID=MsG0380015775.01;Name=MsG0380015775.01
Chr3 mRNA 74890656 74891237 74890656 ID=MsG0380015775.01.T01;Parent=MsG0380015775.01;Name=MsG0380015775.01.T01;_AED=0.03;_eAED=0.03;_QI=0|-1|0|1|-1|1|1|0|193
Chr3 exon 74890656 74891237 74890656 ID=MsG0380015775.01.T01:exon:953;Parent=MsG0380015775.01.T01
Chr3 CDS 74890656 74891237 74890656 ID=MsG0380015775.01.T01:cds;Parent=MsG0380015775.01.T01
Chr3 mRNA 74890719 74891237 74890719 ID=MsG0380015775.01.T02;Parent=MsG0380015775.01;Name=MsG0380015775.01.T02;_AED=0.08;_eAED=0.08;_QI=0|-1|0|1|-1|1|1|0|172
Chr3 exon 74890719 74891237 74890719 ID=MsG0380015775.01.T02:exon:954;Parent=MsG0380015775.01.T02
Chr3 CDS 74890719 74891237 74890719 ID=MsG0380015775.01.T02:cds;Parent=MsG0380015775.01.T02
Gene Sequence

>MsG0380015775.01.T02

ATGCCAGGCTGCATCTTTGCACCATACTTTCCACCAGAAGAACCTCAAAAATTCGCCAACGTTCACAAGATCTTCGGCGCAAGCAACGTCACGAAGATCCTCAACGAACTCCTCCCTCACCAGAGAGAAGATGCAGTGAATTCCCTTGCTTATGAAGCCGAGGCACGTGTGAGAGATCCTGTTTATGGTTGTGTTGGTGCCATCTCTTTCCTTCAGAGACAAGTTCAGAGGCTTCAAAAGGAACTTGATTCTGCTAATGCTGATCTTCTTCGCTTTGCTTACAATGAAATCTCTCCTAATTCCTCTCTTCCACCTCTCCCTCCTCTTGTTGTTCCGGCTTCGTTTCATCAATCTCAAAGACAGTTTAGTTCAAGATTTGGTAATGGAAATGTTGATGGAAATGGATTTTACTCTTCTTTTCCTTATGCTATTCCATGGATTAATGATACCTCTTCTGAGGATATAAGTGGAGGTGTAGGAGGAAGAGGAGGAGGTGTAGGAGGAGGTAATAATTTGTGA

>MsG0380015775.01.T01

ATGGCATCATCAAGCTCATACAATTCACCATGTGCAGCCTGCAAATTCCTTCGTCGAAAATGCATGCCAGGCTGCATCTTTGCACCATACTTTCCACCAGAAGAACCTCAAAAATTCGCCAACGTTCACAAGATCTTCGGCGCAAGCAACGTCACGAAGATCCTCAACGAACTCCTCCCTCACCAGAGAGAAGATGCAGTGAATTCCCTTGCTTATGAAGCCGAGGCACGTGTGAGAGATCCTGTTTATGGTTGTGTTGGTGCCATCTCTTTCCTTCAGAGACAAGTTCAGAGGCTTCAAAAGGAACTTGATTCTGCTAATGCTGATCTTCTTCGCTTTGCTTACAATGAAATCTCTCCTAATTCCTCTCTTCCACCTCTCCCTCCTCTTGTTGTTCCGGCTTCGTTTCATCAATCTCAAAGACAGTTTAGTTCAAGATTTGGTAATGGAAATGTTGATGGAAATGGATTTTACTCTTCTTTTCCTTATGCTATTCCATGGATTAATGATACCTCTTCTGAGGATATAAGTGGAGGTGTAGGAGGAAGAGGAGGAGGTGTAGGAGGAGGTAATAATTTGTGA
Protein sequence

>MsG0380015775.01.T02

MPGCIFAPYFPPEEPQKFANVHKIFGASNVTKILNELLPHQREDAVNSLAYEAEARVRDPVYGCVGAISFLQRQVQRLQKELDSANADLLRFAYNEISPNSSLPPLPPLVVPASFHQSQRQFSSRFGNGNVDGNGFYSSFPYAIPWINDTSSEDISGGVGGRGGGVGGGNNL*

>MsG0380015775.01.T01

MASSSSYNSPCAACKFLRRKCMPGCIFAPYFPPEEPQKFANVHKIFGASNVTKILNELLPHQREDAVNSLAYEAEARVRDPVYGCVGAISFLQRQVQRLQKELDSANADLLRFAYNEISPNSSLPPLPPLVVPASFHQSQRQFSSRFGNGNVDGNGFYSSFPYAIPWINDTSSEDISGGVGGRGGGVGGGNNL*