Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017089.01.T01 | KEH26852.1 | 50.794 | 63 | 31 | 0 | 1 | 63 | 82 | 144 | 7.88E-13 | 72 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017089.01.T01 | A0A072UB72 | 50.794 | 63 | 31 | 0 | 1 | 63 | 82 | 144 | 3.76e-13 | 72.0 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0380017089.01.T01 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017089.01.T01 | MTR_6g477950 | 50.794 | 63 | 31 | 0 | 1 | 63 | 82 | 144 | 9.54e-17 | 72.0 |
| MsG0380017089.01.T01 | MTR_8g069780 | 40.625 | 64 | 38 | 0 | 1 | 64 | 93 | 156 | 4.78e-14 | 62.8 |
| MsG0380017089.01.T01 | MTR_3g117130 | 42.188 | 64 | 37 | 0 | 1 | 64 | 216 | 279 | 1.11e-12 | 60.8 |
| MsG0380017089.01.T01 | MTR_7g117585 | 41.270 | 63 | 37 | 0 | 1 | 63 | 83 | 145 | 2.50e-12 | 58.2 |
| MsG0380017089.01.T01 | MTR_2g437520 | 45.283 | 53 | 29 | 0 | 12 | 64 | 152 | 204 | 4.81e-12 | 58.2 |
| MsG0380017089.01.T01 | MTR_6g034285 | 71.429 | 35 | 10 | 0 | 12 | 46 | 366 | 400 | 4.90e-12 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGATTGGTATGACTTTGTA+AGG | 0.306780 | 3:+91791639 | MsG0380017089.01.T01:CDS |
| TATTACTGCTAAACGTAATA+AGG | 0.321391 | 3:+91791598 | MsG0380017089.01.T01:CDS |
| TGAACAGATAACACTGTAGA+AGG | 0.429582 | 3:-91791710 | None:intergenic |
| ACGACAGTTATACACATAAC+CGG | 0.512452 | 3:-91791573 | None:intergenic |
| ACAAACTGCAGGAAGGTGAC+AGG | 0.543247 | 3:+91791666 | MsG0380017089.01.T01:CDS |
| CCTCAAGCAAGATCATTAGC+AGG | 0.552472 | 3:-91791740 | None:intergenic |
| GTCTTGCTTGATGTGTCGAC+CGG | 0.570735 | 3:+91791554 | MsG0380017089.01.T01:CDS |
| ACTGCAGGAAGGTGACAGGC+TGG | 0.578655 | 3:+91791670 | MsG0380017089.01.T01:CDS |
| CCTGCTAATGATCTTGCTTG+AGG | 0.587271 | 3:+91791740 | MsG0380017089.01.T01:CDS |
| TGTAAGGGATAACAAACTGC+AGG | 0.591831 | 3:+91791655 | MsG0380017089.01.T01:CDS |
| AGATTGGTATGACTTTGTAA+GGG | 0.630399 | 3:+91791640 | MsG0380017089.01.T01:CDS |
| AGGGATAACAAACTGCAGGA+AGG | 0.655927 | 3:+91791659 | MsG0380017089.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAAGGCTAAAAAATCATT+GGG | + | Chr3:91791614-91791633 | MsG0380017089.01.T01:CDS | 20.0% |
| !! | TAATAAGGCTAAAAAATCAT+TGG | + | Chr3:91791613-91791632 | MsG0380017089.01.T01:CDS | 20.0% |
| ! | TATTACTGCTAAACGTAATA+AGG | + | Chr3:91791598-91791617 | MsG0380017089.01.T01:CDS | 25.0% |
| AGATTGGTATGACTTTGTAA+GGG | + | Chr3:91791640-91791659 | MsG0380017089.01.T01:CDS | 30.0% | |
| AAAAATCATTGGGAGGAGAT+TGG | + | Chr3:91791624-91791643 | MsG0380017089.01.T01:CDS | 35.0% | |
| AAGGCTAAAAAATCATTGGG+AGG | + | Chr3:91791617-91791636 | MsG0380017089.01.T01:CDS | 35.0% | |
| ACGACAGTTATACACATAAC+CGG | - | Chr3:91791576-91791595 | None:intergenic | 35.0% | |
| GAGATTGGTATGACTTTGTA+AGG | + | Chr3:91791639-91791658 | MsG0380017089.01.T01:CDS | 35.0% | |
| TGAACAGATAACACTGTAGA+AGG | - | Chr3:91791713-91791732 | None:intergenic | 35.0% | |
| TGTAAGGGATAACAAACTGC+AGG | + | Chr3:91791655-91791674 | MsG0380017089.01.T01:CDS | 40.0% | |
| AGGGATAACAAACTGCAGGA+AGG | + | Chr3:91791659-91791678 | MsG0380017089.01.T01:CDS | 45.0% | |
| ACAAACTGCAGGAAGGTGAC+AGG | + | Chr3:91791666-91791685 | MsG0380017089.01.T01:CDS | 50.0% | |
| ! | GTCTTGCTTGATGTGTCGAC+CGG | + | Chr3:91791554-91791573 | MsG0380017089.01.T01:CDS | 50.0% |
| ACTGCAGGAAGGTGACAGGC+TGG | + | Chr3:91791670-91791689 | MsG0380017089.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 91791551 | 91791760 | 91791551 | ID=MsG0380017089.01;Name=MsG0380017089.01 |
| Chr3 | mRNA | 91791551 | 91791760 | 91791551 | ID=MsG0380017089.01.T01;Parent=MsG0380017089.01;Name=MsG0380017089.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|69 |
| Chr3 | exon | 91791551 | 91791760 | 91791551 | ID=MsG0380017089.01.T01:exon:1188;Parent=MsG0380017089.01.T01 |
| Chr3 | CDS | 91791551 | 91791760 | 91791551 | ID=MsG0380017089.01.T01:cds;Parent=MsG0380017089.01.T01 |
| Gene Sequence |
| Protein sequence |
