AlfalfaGEDB | MsG0380017145.01

M. sativa cultivar ZhongmuNo.1 / MsG0380017145.01

KEGG: K14432
GO: GO:0003700 GO:0006355
PFAM: PF00170

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017145.01.T01	XP_003603049.1	98.058	412	4	2	1	412	1	408	0	805
MsG0380017145.01.T02	XP_003603049.1	98.058	412	4	2	1	412	1	408	0	805

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017145.01.T01	Q9M7Q4	54.177	419	110	15	28	411	21	392	5.43E-115	347
MsG0380017145.01.T02	Q9M7Q4	54.177	419	110	15	28	411	21	392	5.43E-115	347

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017145.01.T01	G7J6E3	98.058	412	4	2	1	412	1	408	0.0	805
MsG0380017145.01.T02	G7J6E3	98.058	412	4	2	1	412	1	408	0.0	805

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TFs/TRs:

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Gene ID	Type	Classification
MsG0380017145.01.T01	TF	bZIP
MsG0380017145.01.T02	TF	bZIP

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017145.01.T01	MTR_3g101780	98.058	412	4	2	1	412	1	408	0.0	805
MsG0380017145.01.T01	MTR_4g085910	46.437	407	135	15	24	411	14	356	9.26e-90	278
MsG0380017145.01.T01	MTR_8g043960	50.407	123	54	2	28	145	24	144	9.62e-29	115
MsG0380017145.01.T01	MTR_8g043960	62.319	69	23	1	337	402	220	288	1.76e-17	83.6
MsG0380017145.01.T01	MTR_6g026800	45.517	145	65	3	15	145	2	146	8.68e-28	113
MsG0380017145.01.T01	MTR_7g104480	47.015	134	49	4	34	145	45	178	3.47e-26	110
MsG0380017145.01.T01	MTR_7g104480	47.287	129	60	3	287	413	280	402	5.69e-13	70.9
MsG0380017145.01.T01	MTR_3g010660	49.180	122	55	2	27	143	19	138	7.01e-26	107
MsG0380017145.01.T01	MTR_3g010660	41.284	109	61	1	297	402	189	297	1.47e-17	84.0
MsG0380017145.01.T01	MTR_7g088090	41.791	134	62	3	28	151	16	143	9.76e-23	99.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017145.01.T01	AT1G45249	54.177	419	110	15	28	411	21	392	5.53e-116	347
MsG0380017145.01.T01	AT1G45249	54.177	419	110	15	28	411	36	407	7.93e-116	347
MsG0380017145.01.T01	AT1G45249	54.177	419	110	15	28	411	36	407	7.93e-116	347
MsG0380017145.01.T01	AT1G45249	52.791	430	110	16	28	411	21	403	1.49e-112	338
MsG0380017145.01.T01	AT1G45249	51.282	390	108	15	28	382	21	363	1.02e-95	293
MsG0380017145.01.T01	AT1G45249	51.282	390	108	15	28	382	21	363	1.02e-95	293
MsG0380017145.01.T01	AT1G45249	51.554	386	105	15	28	378	21	359	2.41e-94	291
MsG0380017145.01.T01	AT1G45249	51.554	386	105	15	28	378	21	359	2.41e-94	291
MsG0380017145.01.T01	AT1G45249	51.554	386	105	15	28	378	21	359	2.41e-94	291
MsG0380017145.01.T01	AT1G45249	51.554	386	105	15	28	378	21	359	2.41e-94	291

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Find 130 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAAAGAGAAGAGAAATC+TGG	0.406441	3:-92572511	None:intergenic
AAATAATGGTAATTTGGGTT+TGG	0.367355	3:+92573531	MsG0380017145.01.T01:CDS
AACAGTTGATGAGGTTTGGA+AGG	0.501243	3:+92573345	MsG0380017145.01.T01:CDS
AAGCAGAGGTGTTGAAACCA+AGG	0.613135	3:+92572294	MsG0380017145.01.T01:five_prime_UTR
AAGGATATTTCTAAGGATTA+TGG	0.280302	3:+92573364	MsG0380017145.01.T01:CDS
AATAAAGTTGCTGGCTTTAT+GGG	0.266563	3:+92573574	MsG0380017145.01.T01:CDS
AATAAATAATATCACAGCAC+AGG	0.541166	3:+92572440	MsG0380017145.01.T01:five_prime_UTR
AATAATCAAGGAATGAGAGG+TGG	0.622320	3:+92573850	MsG0380017145.01.T01:CDS
AATGATCAAGGAGCCTCCGC+CGG	0.545660	3:+92573070	MsG0380017145.01.T01:CDS
AATGGTAATCTTGTGCAAGG+TGG	0.698472	3:+92573904	MsG0380017145.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATCTTTCACCTTTCATC+AGG	-	Chr3:92574733-92574752	None:intergenic	30.0%
AAACAAAGAGAAGAGAAATC+TGG	-	Chr3:92572514-92572533	None:intergenic	30.0%
ACATTCAAAGGCAAATTAGT+AGG	-	Chr3:92573652-92573671	None:intergenic	30.0%
AGAAATCTGGAGAAAGAAAA+AGG	-	Chr3:92572501-92572520	None:intergenic	30.0%
AGAGAAGAATGATCAAGAAT+AGG	+	Chr3:92574124-92574143	MsG0380017145.01.T01:CDS	30.0%
AGAGGAGTAGACATATATAA+GGG	-	Chr3:92574326-92574345	None:intergenic	30.0%
AGTAATCATTTGCAAAGACA+AGG	+	Chr3:92573289-92573308	MsG0380017145.01.T01:CDS	30.0%
AGTTCACTATTAATAGGGTT+TGG	+	Chr3:92574537-92574556	MsG0380017145.01.T01:intron	30.0%
ATGACAATTTGTAAGGATTC+AGG	+	Chr3:92574768-92574787	MsG0380017145.01.T01:intron	30.0%
ATGCCTTTGTCTAATAATCA+AGG	+	Chr3:92573838-92573857	MsG0380017145.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	92572264	92575379	92572264	ID=MsG0380017145.01;Name=MsG0380017145.01
Chr3	mRNA	92572264	92575379	92572264	ID=MsG0380017145.01.T01;Parent=MsG0380017145.01;Name=MsG0380017145.01.T01;_AED=0.34;_eAED=0.41;_QI=228\|1\|0.75\|1\|0.33\|0\|4\|0\|467
Chr3	exon	92572264	92572461	92572264	ID=MsG0380017145.01.T01:exon:20741;Parent=MsG0380017145.01.T01
Chr3	exon	92573019	92574179	92573019	ID=MsG0380017145.01.T01:exon:20742;Parent=MsG0380017145.01.T01
Chr3	five_prime_UTR	92572264	92572461	92572264	ID=MsG0380017145.01.T01:five_prime_utr;Parent=MsG0380017145.01.T01
Chr3	five_prime_UTR	92573019	92573048	92573019	ID=MsG0380017145.01.T01:five_prime_utr;Parent=MsG0380017145.01.T01
Chr3	CDS	92573049	92574179	92573049	ID=MsG0380017145.01.T01:cds;Parent=MsG0380017145.01.T01
Chr3	CDS	92574986	92575057	92574986	ID=MsG0380017145.01.T01:cds;Parent=MsG0380017145.01.T01
Chr3	CDS	92575179	92575379	92575179	ID=MsG0380017145.01.T01:cds;Parent=MsG0380017145.01.T01
Chr3	mRNA	92572458	92575379	92572458	ID=MsG0380017145.01.T02;Parent=MsG0380017145.01;Name=MsG0380017145.01.T02;_AED=0.31;_eAED=0.37;_QI=591\|1\|0.66\|1\|0.5\|0.33\|3\|0\|467
Chr3	exon	92572458	92574179	92572458	ID=MsG0380017145.01.T02:exon:20745;Parent=MsG0380017145.01.T02
Chr3	five_prime_UTR	92572458	92573048	92572458	ID=MsG0380017145.01.T02:five_prime_utr;Parent=MsG0380017145.01.T02
Chr3	CDS	92573049	92574179	92573049	ID=MsG0380017145.01.T02:cds;Parent=MsG0380017145.01.T02
Chr3	CDS	92574986	92575057	92574986	ID=MsG0380017145.01.T02:cds;Parent=MsG0380017145.01.T02
Chr3	CDS	92575179	92575379	92575179	ID=MsG0380017145.01.T02:cds;Parent=MsG0380017145.01.T02

Gene Sequence

>MsG0380017145.01.T02

ATGAATTTCAAGGGTTTTGGGAATGATCAAGGAGCCTCCGCCGGCAACGGAGGTGGGAGGATAGCGGCGGGGAATTTTCCGCTGACGAGGCAACCGTCGGTGTATTCGCTGACGGTTGATGAATTTATGAACAGTATGGGAGGTTCTGGAAAAGATTTTGGGTCAATGAACATGGATGAATTGTTGAAGAATATTTGGAGTGCTGAAGAGGTTCAAACAATGGGTGGTGAAGAAGCTATTAGTAATCATTTGCAAAGACAAGGTTCGTTGACGCTGCCGCGAACTCTTAGTCAGAAAACAGTTGATGAGGTTTGGAAGGATATTTCTAAGGATTATGGTGGTCCTAATTTGGCTGCACCAATGACTCAGAGACAACCAACTTTGGGGGAGATGACTTTGGAAGAGTTTTTGGTTAGAGCTGGTGTTGTTAGAGAAGATGCTAAGCCAAATGATGGTGTTTTTCTTGATCTTGGTAATGTTGGAAATAATGGTAATTTGGGTTTGGCTTTTCAGGCTCAGCAGATGAATAAAGTTGCTGGCTTTATGGGGAATGGTAGTAGAATTAATGGTAATGATGATCCACTTGTTGGTCTTCAAAGTCCTACTAATTTGCCTTTGAATGTTAATGGGATTAGATCAACAAATCAGCAACAACAGATGCAGAATTCACAGTCTCAGGCTCAGCAACAACATCAGAATCAACAACTTCAGCAACTTCAACAGCAGCATCAACAACAGCAGCAGCAGCAAATATTTCCAAAGCAACCTGGTTTGAACTATGCAACTCAGATGCCTTTGTCTAATAATCAAGGAATGAGAGGTGGAATTGTAGGGCTTTCTCCTGATCAAGGATTGAATGGTAATCTTGTGCAAGGTGGAGGGATTGGTATGGTTGGTTTGGCACCAGGCGCTGTTCAAATCGGTGCTGTCTCACCGGCTAACCAGATATCTAGTGATAAAATGGGAAAGAGTAACGGTGATACTTCTTCTGTGTCGCCGGTTCCTTATGTTTTTAATGGTGGTATGCGAGGGAGGAAAGGTAATGGAGCTGTGGAGAAGGTAATTGAGAGGAGGCAGAGAAGAATGATCAAGAATAGGGAGTCAGCTGCTAGATCCCGGGCTCGCAAGCAGGCTTATACCATGGAATTGGAAGCAGAAGTTGCAAAGTTAAAAGAAGAGAATGAAGAACTTCAGAAAAAGCAGGAAGAAATCATGGAACTTCAGAAAAATCAAGTAAGTTCAGCTTTCACAGAAATCGCCGAAAATCATTTGTTCTTGGTTTTAAGTCATTGCGTGCATTTGATTGAATTTACCTTAACTGTACAAGTCTCTAGTTTTTCTAGAACACATAGCAGTGTCCTTGTGCCCTGTTTTGTGACTAATCGGTTAAGAAGTCATGAATAG

>MsG0380017145.01.T01

Protein sequence

>MsG0380017145.01.T02

MNFKGFGNDQGASAGNGGGRIAAGNFPLTRQPSVYSLTVDEFMNSMGGSGKDFGSMNMDELLKNIWSAEEVQTMGGEEAISNHLQRQGSLTLPRTLSQKTVDEVWKDISKDYGGPNLAAPMTQRQPTLGEMTLEEFLVRAGVVREDAKPNDGVFLDLGNVGNNGNLGLAFQAQQMNKVAGFMGNGSRINGNDDPLVGLQSPTNLPLNVNGIRSTNQQQQMQNSQSQAQQQHQNQQLQQLQQQHQQQQQQQIFPKQPGLNYATQMPLSNNQGMRGGIVGLSPDQGLNGNLVQGGGIGMVGLAPGAVQIGAVSPANQISSDKMGKSNGDTSSVSPVPYVFNGGMRGRKGNGAVEKVIERRQRRMIKNRESAARSRARKQAYTMELEAEVAKLKEENEELQKKQEEIMELQKNQVSSAFTEIAENHLFLVLSHCVHLIEFTLTVQVSSFSRTHSSVLVPCFVTNRLRSHE*

>MsG0380017145.01.T01