Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017248.01.T01 | XP_013461831.1 | 84.818 | 303 | 27 | 5 | 1 | 294 | 12 | 304 | 2.10E-153 | 441 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017248.01.T01 | A2XE76 | 54.696 | 181 | 61 | 3 | 83 | 248 | 81 | 255 | 9.15E-56 | 185 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017248.01.T01 | A0A072V2J8 | 84.818 | 303 | 27 | 5 | 1 | 294 | 12 | 304 | 1.00e-153 | 441 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0380017248.01.T01 | TF | HB-HD-ZIP |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017248.01.T01 | MTR_3g103590 | 84.818 | 303 | 27 | 5 | 1 | 294 | 12 | 304 | 2.54e-157 | 441 |
| MsG0380017248.01.T01 | MTR_1g017090 | 50.171 | 293 | 120 | 11 | 6 | 290 | 11 | 285 | 3.63e-76 | 234 |
| MsG0380017248.01.T01 | MTR_5g014890 | 60.736 | 163 | 57 | 4 | 124 | 286 | 114 | 269 | 9.04e-59 | 189 |
| MsG0380017248.01.T01 | MTR_7g093430 | 69.841 | 126 | 35 | 2 | 123 | 248 | 150 | 272 | 9.44e-56 | 182 |
| MsG0380017248.01.T01 | MTR_1g054285 | 50.746 | 201 | 89 | 4 | 50 | 250 | 78 | 268 | 4.54e-55 | 180 |
| MsG0380017248.01.T01 | MTR_4g097600 | 66.142 | 127 | 40 | 2 | 122 | 248 | 116 | 239 | 2.17e-53 | 175 |
| MsG0380017248.01.T01 | MTR_2g061030 | 65.116 | 129 | 42 | 2 | 124 | 252 | 191 | 316 | 4.88e-51 | 171 |
| MsG0380017248.01.T01 | MTR_4g100550 | 54.967 | 151 | 64 | 2 | 123 | 272 | 110 | 257 | 2.74e-50 | 167 |
| MsG0380017248.01.T01 | MTR_5g013010 | 52.326 | 172 | 77 | 3 | 95 | 265 | 99 | 266 | 4.27e-50 | 167 |
| MsG0380017248.01.T01 | MTR_8g006705 | 68.376 | 117 | 33 | 2 | 132 | 247 | 145 | 258 | 3.20e-49 | 166 |
| MsG0380017248.01.T01 | MTR_4g084750 | 55.118 | 127 | 54 | 1 | 124 | 247 | 56 | 182 | 3.69e-42 | 144 |
| MsG0380017248.01.T01 | MTR_2g038625 | 49.102 | 167 | 74 | 4 | 104 | 264 | 50 | 211 | 2.37e-39 | 137 |
| MsG0380017248.01.T01 | MTR_2g038580 | 49.102 | 167 | 74 | 4 | 104 | 264 | 50 | 211 | 2.37e-39 | 137 |
| MsG0380017248.01.T01 | MTR_4g126900 | 56.557 | 122 | 36 | 4 | 135 | 247 | 90 | 203 | 2.70e-32 | 119 |
| MsG0380017248.01.T01 | MTR_5g013010 | 69.355 | 62 | 19 | 0 | 123 | 184 | 126 | 187 | 1.01e-21 | 90.9 |
| MsG0380017248.01.T01 | MTR_1g061660 | 43.820 | 89 | 50 | 0 | 133 | 221 | 59 | 147 | 1.65e-18 | 82.8 |
| MsG0380017248.01.T01 | MTR_6g007647 | 48.352 | 91 | 47 | 0 | 130 | 220 | 30 | 120 | 2.22e-18 | 82.8 |
| MsG0380017248.01.T01 | MTR_6g007647 | 48.352 | 91 | 47 | 0 | 130 | 220 | 64 | 154 | 2.67e-18 | 83.2 |
| MsG0380017248.01.T01 | MTR_5g039000 | 40.351 | 114 | 58 | 2 | 121 | 224 | 62 | 175 | 5.30e-17 | 79.7 |
| MsG0380017248.01.T01 | MTR_7g010020 | 40.952 | 105 | 55 | 1 | 123 | 220 | 70 | 174 | 6.49e-17 | 79.3 |
| MsG0380017248.01.T01 | MTR_7g103340 | 39.604 | 101 | 60 | 1 | 121 | 220 | 50 | 150 | 2.31e-16 | 77.0 |
| MsG0380017248.01.T01 | MTR_5g038280 | 45.745 | 94 | 44 | 1 | 133 | 219 | 87 | 180 | 2.32e-16 | 78.6 |
| MsG0380017248.01.T01 | MTR_4g107650 | 37.879 | 132 | 77 | 1 | 89 | 220 | 56 | 182 | 4.00e-16 | 77.8 |
| MsG0380017248.01.T01 | MTR_8g469430 | 46.809 | 94 | 43 | 1 | 133 | 219 | 89 | 182 | 5.66e-16 | 76.6 |
| MsG0380017248.01.T01 | MTR_8g468210 | 40.000 | 100 | 57 | 1 | 124 | 220 | 65 | 164 | 9.91e-16 | 75.9 |
| MsG0380017248.01.T01 | MTR_3g086790 | 45.361 | 97 | 46 | 1 | 131 | 220 | 58 | 154 | 1.01e-15 | 76.3 |
| MsG0380017248.01.T01 | MTR_3g080100 | 36.567 | 134 | 72 | 3 | 133 | 259 | 20 | 147 | 6.22e-15 | 72.8 |
| MsG0380017248.01.T01 | MTR_3g080100 | 36.567 | 134 | 72 | 3 | 133 | 259 | 59 | 186 | 7.36e-15 | 73.2 |
| MsG0380017248.01.T01 | MTR_6g011610 | 44.681 | 94 | 45 | 1 | 133 | 219 | 96 | 189 | 8.92e-15 | 73.2 |
| MsG0380017248.01.T01 | MTR_8g089895 | 40.909 | 110 | 61 | 1 | 121 | 226 | 42 | 151 | 1.39e-14 | 73.2 |
| MsG0380017248.01.T01 | MTR_6g011610 | 44.681 | 94 | 45 | 1 | 133 | 219 | 77 | 170 | 1.68e-14 | 72.0 |
| MsG0380017248.01.T01 | MTR_8g026960 | 37.000 | 100 | 60 | 1 | 137 | 236 | 38 | 134 | 1.60e-12 | 66.2 |
| MsG0380017248.01.T01 | MTR_3g092150 | 43.529 | 85 | 48 | 0 | 136 | 220 | 41 | 125 | 4.55e-12 | 65.1 |
| MsG0380017248.01.T01 | MTR_5g019650 | 43.158 | 95 | 49 | 1 | 123 | 217 | 48 | 137 | 8.93e-12 | 63.9 |
| MsG0380017248.01.T01 | MTR_5g019680 | 39.423 | 104 | 58 | 1 | 123 | 226 | 48 | 146 | 5.02e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017248.01.T01 | AT5G06710 | 58.788 | 165 | 60 | 4 | 89 | 253 | 151 | 307 | 6.04e-56 | 184 |
| MsG0380017248.01.T01 | AT4G37790 | 69.291 | 127 | 36 | 2 | 122 | 248 | 115 | 238 | 6.22e-55 | 179 |
| MsG0380017248.01.T01 | AT2G22800 | 68.800 | 125 | 36 | 2 | 124 | 248 | 104 | 225 | 5.86e-53 | 174 |
| MsG0380017248.01.T01 | AT4G16780 | 41.154 | 260 | 110 | 4 | 42 | 266 | 9 | 260 | 1.95e-52 | 173 |
| MsG0380017248.01.T01 | AT3G60390 | 64.567 | 127 | 41 | 2 | 122 | 247 | 151 | 274 | 3.96e-50 | 168 |
| MsG0380017248.01.T01 | AT5G47370 | 61.719 | 128 | 45 | 2 | 122 | 248 | 119 | 243 | 3.78e-47 | 159 |
| MsG0380017248.01.T01 | AT4G17460 | 61.905 | 126 | 44 | 2 | 124 | 248 | 126 | 248 | 4.10e-47 | 159 |
| MsG0380017248.01.T01 | AT2G44910 | 64.407 | 118 | 38 | 2 | 132 | 248 | 162 | 276 | 2.35e-46 | 159 |
| MsG0380017248.01.T01 | AT2G01430 | 55.639 | 133 | 48 | 3 | 122 | 248 | 122 | 249 | 8.82e-41 | 143 |
| MsG0380017248.01.T01 | AT1G70920 | 46.939 | 147 | 66 | 3 | 121 | 266 | 26 | 161 | 1.78e-35 | 126 |
| MsG0380017248.01.T01 | AT1G70920 | 50.388 | 129 | 53 | 2 | 121 | 248 | 56 | 174 | 1.98e-35 | 127 |
| MsG0380017248.01.T01 | AT5G06710 | 57.292 | 96 | 36 | 2 | 89 | 184 | 151 | 241 | 7.34e-24 | 98.2 |
| MsG0380017248.01.T01 | AT2G01430 | 62.319 | 69 | 20 | 2 | 122 | 184 | 122 | 190 | 3.56e-19 | 84.3 |
| MsG0380017248.01.T01 | AT3G01470 | 54.167 | 72 | 33 | 0 | 133 | 204 | 68 | 139 | 2.02e-16 | 77.8 |
| MsG0380017248.01.T01 | AT5G03790 | 40.000 | 95 | 57 | 0 | 127 | 221 | 71 | 165 | 6.23e-16 | 75.9 |
| MsG0380017248.01.T01 | AT1G69780 | 40.541 | 111 | 56 | 2 | 124 | 224 | 73 | 183 | 7.01e-16 | 76.6 |
| MsG0380017248.01.T01 | AT2G18550 | 46.154 | 91 | 49 | 0 | 129 | 219 | 57 | 147 | 3.72e-15 | 73.6 |
| MsG0380017248.01.T01 | AT5G15150 | 40.741 | 108 | 52 | 2 | 124 | 219 | 101 | 208 | 4.62e-15 | 74.7 |
| MsG0380017248.01.T01 | AT2G36610 | 35.714 | 126 | 66 | 3 | 109 | 221 | 44 | 167 | 6.90e-15 | 72.0 |
| MsG0380017248.01.T01 | AT1G27045 | 51.389 | 72 | 35 | 0 | 133 | 204 | 25 | 96 | 1.23e-14 | 71.2 |
| MsG0380017248.01.T01 | AT1G27045 | 51.389 | 72 | 35 | 0 | 133 | 204 | 25 | 96 | 1.23e-14 | 71.2 |
| MsG0380017248.01.T01 | AT1G27045 | 51.389 | 72 | 35 | 0 | 133 | 204 | 17 | 88 | 1.30e-14 | 70.9 |
| MsG0380017248.01.T01 | AT1G27045 | 51.389 | 72 | 35 | 0 | 133 | 204 | 68 | 139 | 1.31e-14 | 72.0 |
| MsG0380017248.01.T01 | AT1G26960 | 44.318 | 88 | 49 | 0 | 133 | 220 | 71 | 158 | 1.47e-14 | 72.4 |
| MsG0380017248.01.T01 | AT5G03790 | 36.066 | 122 | 73 | 1 | 127 | 243 | 71 | 192 | 1.54e-14 | 71.2 |
| MsG0380017248.01.T01 | AT1G27045 | 51.389 | 72 | 35 | 0 | 133 | 204 | 68 | 139 | 2.00e-14 | 72.0 |
| MsG0380017248.01.T01 | AT4G40060 | 40.187 | 107 | 56 | 2 | 131 | 230 | 57 | 162 | 2.25e-14 | 72.4 |
| MsG0380017248.01.T01 | AT5G65310 | 42.857 | 98 | 49 | 1 | 130 | 220 | 51 | 148 | 3.18e-14 | 72.0 |
| MsG0380017248.01.T01 | AT5G65310 | 42.857 | 98 | 49 | 1 | 130 | 220 | 69 | 166 | 3.73e-14 | 72.0 |
| MsG0380017248.01.T01 | AT3G01220 | 35.593 | 118 | 66 | 2 | 99 | 211 | 53 | 165 | 4.63e-14 | 71.2 |
| MsG0380017248.01.T01 | AT2G22430 | 42.268 | 97 | 49 | 1 | 131 | 220 | 60 | 156 | 5.48e-14 | 71.6 |
| MsG0380017248.01.T01 | AT4G36740 | 40.816 | 98 | 58 | 0 | 129 | 226 | 51 | 148 | 8.99e-14 | 69.3 |
| MsG0380017248.01.T01 | AT4G36740 | 40.816 | 98 | 58 | 0 | 129 | 226 | 52 | 149 | 9.04e-14 | 69.3 |
| MsG0380017248.01.T01 | AT3G61890 | 37.000 | 100 | 60 | 1 | 136 | 235 | 33 | 129 | 5.70e-12 | 64.7 |
| MsG0380017248.01.T01 | AT2G46680 | 37.895 | 95 | 55 | 1 | 136 | 226 | 35 | 129 | 8.97e-12 | 64.3 |
| MsG0380017248.01.T01 | AT2G46680 | 40.000 | 85 | 51 | 0 | 136 | 220 | 35 | 119 | 1.78e-11 | 63.5 |
Find 84 sgRNAs with CRISPR-Local
Find 114 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCTCTTCAATATTATTATT+AGG | 0.146207 | 3:-94036411 | None:intergenic |
| TCTCTTCAATATTATTATTA+GGG | 0.203525 | 3:-94036410 | None:intergenic |
| TATTTGCATAGAAGTGAATT+TGG | 0.206531 | 3:-94037881 | None:intergenic |
| ACTCCTAAGGTAAGAGAATA+TGG | 0.209934 | 3:-94036381 | None:intergenic |
| TATATACAGTACTCATTAAT+TGG | 0.243867 | 3:+94038007 | MsG0380017248.01.T01:three_prime_UTR |
| CTGGCTCATGATGTACTTCT+TGG | 0.248157 | 3:-94036587 | None:intergenic |
| CAAGATTGAGGATTGTTCTT+TGG | 0.279678 | 3:-94036676 | None:intergenic |
| TAACAGTCTACAAATTAAAA+TGG | 0.284021 | 3:+94038223 | MsG0380017248.01.T01:three_prime_UTR |
| ATTTCTCTTGGTCTTGGTCT+TGG | 0.292030 | 3:+94036285 | MsG0380017248.01.T01:CDS |
| AAGCTGCATGTAATTATTTA+CGG | 0.292324 | 3:-94037745 | None:intergenic |
| CTCCCTCTTGATGCTATTAG+AGG | 0.294156 | 3:-94036547 | None:intergenic |
| TAGAAGTGAATTTGGGCCTT+TGG | 0.313028 | 3:-94037873 | None:intergenic |
| TTTGATATTTCTCTTGGTCT+TGG | 0.326125 | 3:+94036279 | MsG0380017248.01.T01:CDS |
| TAAACAAAGCAAGAAGATTA+AGG | 0.355539 | 3:+94036326 | MsG0380017248.01.T01:CDS |
| AGCAATAGTTGCAGAAGATT+TGG | 0.363873 | 3:-94037922 | None:intergenic |
| CGATCCTCATATTCAATAAT+CGG | 0.372761 | 3:-94038059 | None:intergenic |
| AGAAGTGAATTTGGGCCTTT+GGG | 0.375155 | 3:-94037872 | None:intergenic |
| AGAGGAGGATCCATGTAATA+AGG | 0.377051 | 3:+94036353 | MsG0380017248.01.T01:CDS |
| AGCTGCATGTAATTATTTAC+GGG | 0.380708 | 3:-94037744 | None:intergenic |
| TTGCAATTAGTTTCATTTCG+TGG | 0.382499 | 3:+94038286 | MsG0380017248.01.T01:three_prime_UTR |
| AAATAATTACATGCAGCTTC+CGG | 0.384069 | 3:+94037749 | MsG0380017248.01.T01:CDS |
| ATCATTGAGATTCACATAAT+CGG | 0.388436 | 3:-94038145 | None:intergenic |
| AAGCCTAAGTTTCTTCCTAC+TGG | 0.393408 | 3:-94036652 | None:intergenic |
| AAGAGAAATATCAAAATTCA+TGG | 0.395654 | 3:-94036271 | None:intergenic |
| ATTTGCATAGAAGTGAATTT+GGG | 0.397943 | 3:-94037880 | None:intergenic |
| GCAACTATTGCTAGCTAGCT+AGG | 0.400487 | 3:+94037933 | MsG0380017248.01.T01:exon |
| CATCACGCTAGTAGAATTAG+AGG | 0.402844 | 3:-94036508 | None:intergenic |
| CTCATCGCCGGTGCCGGTGC+CGG | 0.407320 | 3:-94037814 | None:intergenic |
| GAGAGTAGAATGATCTTTGA+AGG | 0.413896 | 3:-94036706 | None:intergenic |
| TGGAGGTGTGGTTTCAAAAC+AGG | 0.418208 | 3:+94037217 | MsG0380017248.01.T01:CDS |
| CAAAGAACAATCCTCAATCT+TGG | 0.418520 | 3:+94036677 | MsG0380017248.01.T01:CDS |
| TCAACAAATGAAATCTTCTC+TGG | 0.427530 | 3:-94036606 | None:intergenic |
| GTTTATGTCTTTGTTCATGA+AGG | 0.439832 | 3:-94036308 | None:intergenic |
| TCAGAAGCAAAAGCAAGAAT+TGG | 0.442692 | 3:+94037164 | MsG0380017248.01.T01:intron |
| CAAGAAGATTAAGGAGGAAG+AGG | 0.445576 | 3:+94036335 | MsG0380017248.01.T01:CDS |
| CTTGTGCCACTCATAAAGTA+TGG | 0.454774 | 3:+94038249 | MsG0380017248.01.T01:three_prime_UTR |
| CTTGATGCTATTAGAGGAGT+TGG | 0.458659 | 3:-94036541 | None:intergenic |
| GCCATTCTCATCGCCGGTGC+CGG | 0.463859 | 3:-94037820 | None:intergenic |
| TATTCTCTTACCTTAGGAGT+TGG | 0.474125 | 3:+94036384 | MsG0380017248.01.T01:CDS |
| TGGCAAGCAAGCTGAATCTT+CGG | 0.477653 | 3:+94037184 | MsG0380017248.01.T01:CDS |
| TACTACAACAAAGACTGAAT+CGG | 0.478308 | 3:+94036455 | MsG0380017248.01.T01:CDS |
| TACCCATATTCTCTTACCTT+AGG | 0.484324 | 3:+94036378 | MsG0380017248.01.T01:CDS |
| ATTTGCACCGGCACCGGCAC+CGG | 0.484691 | 3:+94037807 | MsG0380017248.01.T01:CDS |
| GAAGGTGTCCTCCAAGATTG+AGG | 0.485914 | 3:-94036688 | None:intergenic |
| TCACGTACGTGCTGCTTATG+TGG | 0.499042 | 3:+94038103 | MsG0380017248.01.T01:three_prime_UTR |
| ATAGCCGATTATTGAATATG+AGG | 0.499399 | 3:+94038055 | MsG0380017248.01.T01:three_prime_UTR |
| ACCGGCACCGGCGATGAGAA+TGG | 0.503337 | 3:+94037819 | MsG0380017248.01.T01:CDS |
| TATGGGTATGCCTTATTACA+TGG | 0.508332 | 3:-94036363 | None:intergenic |
| CTCCTAAGGTAAGAGAATAT+GGG | 0.512060 | 3:-94036380 | None:intergenic |
| AAAAGAATTTGCACCGGCAC+CGG | 0.514546 | 3:+94037801 | MsG0380017248.01.T01:CDS |
| AATCAAAGCCGTGGTGTGAG+AGG | 0.529506 | 3:-94037844 | None:intergenic |
| AAAGCTGAAACAAACGGAAG+TGG | 0.530773 | 3:+94037620 | MsG0380017248.01.T01:CDS |
| GGCTTTGATTCTCTGCCCAA+AGG | 0.536458 | 3:+94037857 | MsG0380017248.01.T01:CDS |
| CTCTTCAATATTATTATTAG+GGG | 0.543753 | 3:-94036409 | None:intergenic |
| CTCTAACAGAAGAGAACAAG+AGG | 0.544383 | 3:+94037673 | MsG0380017248.01.T01:CDS |
| CAGGACAAAGCTGAAACAAA+CGG | 0.546061 | 3:+94037614 | MsG0380017248.01.T01:intron |
| AGATCATTCTACTCTCAATC+CGG | 0.548356 | 3:+94036713 | MsG0380017248.01.T01:CDS |
| AGAAGGGCAAACTGTAAGAC+TGG | 0.550866 | 3:-94037775 | None:intergenic |
| ACTCCAGTAGGAAGAAACTT+AGG | 0.556676 | 3:+94036649 | MsG0380017248.01.T01:CDS |
| GGACGAGACAAGTGGAGGTG+TGG | 0.566994 | 3:+94037205 | MsG0380017248.01.T01:CDS |
| CAACTATTGCTAGCTAGCTA+GGG | 0.567943 | 3:+94037934 | MsG0380017248.01.T01:exon |
| CAAACTGTAAGACTGGCAAC+CGG | 0.573412 | 3:-94037768 | None:intergenic |
| ATTAGGGGATCCAACTCCTA+AGG | 0.574104 | 3:-94036394 | None:intergenic |
| GAAGATTAAGGAGGAAGAGG+AGG | 0.576607 | 3:+94036338 | MsG0380017248.01.T01:CDS |
| TTGTTACCATACTTTATGAG+TGG | 0.581833 | 3:-94038255 | None:intergenic |
| AGAGGAGCCATTCTCATCGC+CGG | 0.585404 | 3:-94037826 | None:intergenic |
| GGTTTCAAAACAGGAGGGCA+AGG | 0.585888 | 3:+94037226 | MsG0380017248.01.T01:CDS |
| AGAACAATCCTCAATCTTGG+AGG | 0.594021 | 3:+94036680 | MsG0380017248.01.T01:CDS |
| CGATTATTGAATATGAGGAT+CGG | 0.601223 | 3:+94038060 | MsG0380017248.01.T01:three_prime_UTR |
| AGGTGTGGTTTCAAAACAGG+AGG | 0.617414 | 3:+94037220 | MsG0380017248.01.T01:CDS |
| AGAGAACAAGAGGCTAGAAG+AGG | 0.618606 | 3:+94037683 | MsG0380017248.01.T01:CDS |
| ACTCCTCTAATAGCATCAAG+AGG | 0.625878 | 3:+94036544 | MsG0380017248.01.T01:CDS |
| ATGTAAATGATAACTCCAGT+AGG | 0.626226 | 3:+94036637 | MsG0380017248.01.T01:CDS |
| TCTTGTAAAAGAATTTGCAC+CGG | 0.632422 | 3:+94037795 | MsG0380017248.01.T01:CDS |
| GAATCTTCGGACGAGACAAG+TGG | 0.639016 | 3:+94037197 | MsG0380017248.01.T01:CDS |
| CATACTTTATGAGTGGCACA+AGG | 0.641148 | 3:-94038248 | None:intergenic |
| CTCCTCTAATAGCATCAAGA+GGG | 0.641427 | 3:+94036545 | MsG0380017248.01.T01:CDS |
| ACAAAGCAAGAAGATTAAGG+AGG | 0.671987 | 3:+94036329 | MsG0380017248.01.T01:CDS |
| TGGGCAGAGAATCAAAGCCG+TGG | 0.678880 | 3:-94037853 | None:intergenic |
| TCTTCGGACGAGACAAGTGG+AGG | 0.693782 | 3:+94037200 | MsG0380017248.01.T01:CDS |
| ACAAACGGAAGTGGACTGTG+AGG | 0.696543 | 3:+94037629 | MsG0380017248.01.T01:CDS |
| GGTGTGGTTTCAAAACAGGA+GGG | 0.700214 | 3:+94037221 | MsG0380017248.01.T01:CDS |
| GCTTAAGTCCATGAAAACAA+CGG | 0.721053 | 3:+94037716 | MsG0380017248.01.T01:CDS |
| GAATGGCTCCTCTCACACCA+CGG | 0.757581 | 3:+94037836 | MsG0380017248.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAATTAATAAAATAAA+TGG | - | Chr3:94036948-94036967 | None:intergenic | 0.0% |
| !!! | TATTATTATAAATATTTTTA+TGG | + | Chr3:94037299-94037318 | MsG0380017248.01.T01:intron | 0.0% |
| !! | TAATATTATGTAAAAAAACA+TGG | - | Chr3:94037284-94037303 | None:intergenic | 10.0% |
| !!! | AAATTGAATAAATTTTTTGA+TGG | + | Chr3:94037025-94037044 | MsG0380017248.01.T01:intron | 10.0% |
| !!! | ATTATAAATATTTTTATGGT+TGG | + | Chr3:94037303-94037322 | MsG0380017248.01.T01:intron | 10.0% |
| !! | ATAACATCATATTATATAGT+AGG | - | Chr3:94037096-94037115 | None:intergenic | 15.0% |
| !! | ATTTATTTGAGCAAATTAAT+TGG | + | Chr3:94036786-94036805 | MsG0380017248.01.T01:intron | 15.0% |
| !! | TAAATTAACTAATATGTACT+TGG | + | Chr3:94036758-94036777 | MsG0380017248.01.T01:intron | 15.0% |
| !! | TAACATCATATTATATAGTA+GGG | - | Chr3:94037095-94037114 | None:intergenic | 15.0% |
| !! | TCTCTTCAATATTATTATTA+GGG | - | Chr3:94036413-94036432 | None:intergenic | 15.0% |
| !!! | CAAAGATATTAGTTTTTTTT+AGG | + | Chr3:94036845-94036864 | MsG0380017248.01.T01:intron | 15.0% |
| !! | AAGAATTTGAAGATATATAC+CGG | - | Chr3:94036735-94036754 | None:intergenic | 20.0% |
| !! | AAGAGAAATATCAAAATTCA+TGG | - | Chr3:94036274-94036293 | None:intergenic | 20.0% |
| !! | ATTTACCATATAATATATGC+AGG | + | Chr3:94037595-94037614 | MsG0380017248.01.T01:intron | 20.0% |
| !! | CTCTTCAATATTATTATTAG+GGG | - | Chr3:94036412-94036431 | None:intergenic | 20.0% |
| !! | GTCTCTTCAATATTATTATT+AGG | - | Chr3:94036414-94036433 | None:intergenic | 20.0% |
| !! | GTTATTTATAAGTCATGAAT+TGG | + | Chr3:94037111-94037130 | MsG0380017248.01.T01:intron | 20.0% |
| !! | TAACAGTCTACAAATTAAAA+TGG | + | Chr3:94038223-94038242 | MsG0380017248.01.T01:three_prime_UTR | 20.0% |
| !! | TATATACAGTACTCATTAAT+TGG | + | Chr3:94038007-94038026 | MsG0380017248.01.T01:three_prime_UTR | 20.0% |
| !! | TTAATAAAAATGAGTGCATT+TGG | + | Chr3:94036968-94036987 | MsG0380017248.01.T01:intron | 20.0% |
| !! | TTTAATTACTTTGCTTTACA+AGG | - | Chr3:94037008-94037027 | None:intergenic | 20.0% |
| !!! | ATGAATTTTGATATTTCTCT+TGG | + | Chr3:94036273-94036292 | MsG0380017248.01.T01:CDS | 20.0% |
| !!! | ATTAATCTTTTGAATCTTGT+GGG | - | Chr3:94037393-94037412 | None:intergenic | 20.0% |
| ! | AAGCTGCATGTAATTATTTA+CGG | - | Chr3:94037748-94037767 | None:intergenic | 25.0% |
| ! | ATCATTGAGATTCACATAAT+CGG | - | Chr3:94038148-94038167 | None:intergenic | 25.0% |
| ! | ATTTGCATAGAAGTGAATTT+GGG | - | Chr3:94037883-94037902 | None:intergenic | 25.0% |
| ! | TAAACAAAGCAAGAAGATTA+AGG | + | Chr3:94036326-94036345 | MsG0380017248.01.T01:CDS | 25.0% |
| ! | TATTTGCATAGAAGTGAATT+TGG | - | Chr3:94037884-94037903 | None:intergenic | 25.0% |
| ! | TTTGTCCTGCATATATTATA+TGG | - | Chr3:94037603-94037622 | None:intergenic | 25.0% |
| !! | AACTATCCTTAAGAGTTTTA+AGG | - | Chr3:94037354-94037373 | None:intergenic | 25.0% |
| !! | GATTAATCTTTTGAATCTTG+TGG | - | Chr3:94037394-94037413 | None:intergenic | 25.0% |
| !! | TGCAAATTCTTTTACAAGAA+GGG | - | Chr3:94037794-94037813 | None:intergenic | 25.0% |
| AAATAATTACATGCAGCTTC+CGG | + | Chr3:94037749-94037768 | MsG0380017248.01.T01:CDS | 30.0% | |
| AGCTGCATGTAATTATTTAC+GGG | - | Chr3:94037747-94037766 | None:intergenic | 30.0% | |
| ATAGCCGATTATTGAATATG+AGG | + | Chr3:94038055-94038074 | MsG0380017248.01.T01:three_prime_UTR | 30.0% | |
| ATGTAAATGATAACTCCAGT+AGG | + | Chr3:94036637-94036656 | MsG0380017248.01.T01:CDS | 30.0% | |
| CGATCCTCATATTCAATAAT+CGG | - | Chr3:94038062-94038081 | None:intergenic | 30.0% | |
| CGATTATTGAATATGAGGAT+CGG | + | Chr3:94038060-94038079 | MsG0380017248.01.T01:three_prime_UTR | 30.0% | |
| CTTTACAAGGACTAAATACA+TGG | - | Chr3:94036995-94037014 | None:intergenic | 30.0% | |
| GTTTATGTCTTTGTTCATGA+AGG | - | Chr3:94036311-94036330 | None:intergenic | 30.0% | |
| TACTACAACAAAGACTGAAT+CGG | + | Chr3:94036455-94036474 | MsG0380017248.01.T01:CDS | 30.0% | |
| TCAACAAATGAAATCTTCTC+TGG | - | Chr3:94036609-94036628 | None:intergenic | 30.0% | |
| TCTTGTAAAAGAATTTGCAC+CGG | + | Chr3:94037795-94037814 | MsG0380017248.01.T01:CDS | 30.0% | |
| TTGTTACCATACTTTATGAG+TGG | - | Chr3:94038258-94038277 | None:intergenic | 30.0% | |
| TTTGATATTTCTCTTGGTCT+TGG | + | Chr3:94036279-94036298 | MsG0380017248.01.T01:CDS | 30.0% | |
| ! | GTGCAAATTCTTTTACAAGA+AGG | - | Chr3:94037795-94037814 | None:intergenic | 30.0% |
| ! | TTGCAATTAGTTTCATTTCG+TGG | + | Chr3:94038286-94038305 | MsG0380017248.01.T01:three_prime_UTR | 30.0% |
| AAGCGACCTTAAAACTCTTA+AGG | + | Chr3:94037345-94037364 | MsG0380017248.01.T01:intron | 35.0% | |
| ACAAAGCAAGAAGATTAAGG+AGG | + | Chr3:94036329-94036348 | MsG0380017248.01.T01:CDS | 35.0% | |
| ACTCCTAAGGTAAGAGAATA+TGG | - | Chr3:94036384-94036403 | None:intergenic | 35.0% | |
| AGATCATTCTACTCTCAATC+CGG | + | Chr3:94036713-94036732 | MsG0380017248.01.T01:CDS | 35.0% | |
| AGCAATAGTTGCAGAAGATT+TGG | - | Chr3:94037925-94037944 | None:intergenic | 35.0% | |
| CAAAGAACAATCCTCAATCT+TGG | + | Chr3:94036677-94036696 | MsG0380017248.01.T01:CDS | 35.0% | |
| CAAGATTGAGGATTGTTCTT+TGG | - | Chr3:94036679-94036698 | None:intergenic | 35.0% | |
| CTCCTAAGGTAAGAGAATAT+GGG | - | Chr3:94036383-94036402 | None:intergenic | 35.0% | |
| GAGAGTAGAATGATCTTTGA+AGG | - | Chr3:94036709-94036728 | None:intergenic | 35.0% | |
| GCTTAAGTCCATGAAAACAA+CGG | + | Chr3:94037716-94037735 | MsG0380017248.01.T01:CDS | 35.0% | |
| TACCCATATTCTCTTACCTT+AGG | + | Chr3:94036378-94036397 | MsG0380017248.01.T01:CDS | 35.0% | |
| TATTCTCTTACCTTAGGAGT+TGG | + | Chr3:94036384-94036403 | MsG0380017248.01.T01:CDS | 35.0% | |
| TCAGAAGCAAAAGCAAGAAT+TGG | + | Chr3:94037164-94037183 | MsG0380017248.01.T01:intron | 35.0% | |
| TGCTTCTGATCATATGTCAA+AGG | - | Chr3:94037153-94037172 | None:intergenic | 35.0% | |
| ! | TATGGGTATGCCTTATTACA+TGG | - | Chr3:94036366-94036385 | None:intergenic | 35.0% |
| AAAGCTGAAACAAACGGAAG+TGG | + | Chr3:94037620-94037639 | MsG0380017248.01.T01:CDS | 40.0% | |
| AAGCCTAAGTTTCTTCCTAC+TGG | - | Chr3:94036655-94036674 | None:intergenic | 40.0% | |
| ACTCCAGTAGGAAGAAACTT+AGG | + | Chr3:94036649-94036668 | MsG0380017248.01.T01:CDS | 40.0% | |
| ACTCCTCTAATAGCATCAAG+AGG | + | Chr3:94036544-94036563 | MsG0380017248.01.T01:CDS | 40.0% | |
| AGAACAATCCTCAATCTTGG+AGG | + | Chr3:94036680-94036699 | MsG0380017248.01.T01:CDS | 40.0% | |
| AGAAGTGAATTTGGGCCTTT+GGG | - | Chr3:94037875-94037894 | None:intergenic | 40.0% | |
| AGAGGAGGATCCATGTAATA+AGG | + | Chr3:94036353-94036372 | MsG0380017248.01.T01:CDS | 40.0% | |
| CAACTATTGCTAGCTAGCTA+GGG | + | Chr3:94037934-94037953 | MsG0380017248.01.T01:exon | 40.0% | |
| CAAGAAGATTAAGGAGGAAG+AGG | + | Chr3:94036335-94036354 | MsG0380017248.01.T01:CDS | 40.0% | |
| CAGGACAAAGCTGAAACAAA+CGG | + | Chr3:94037614-94037633 | MsG0380017248.01.T01:intron | 40.0% | |
| CATACTTTATGAGTGGCACA+AGG | - | Chr3:94038251-94038270 | None:intergenic | 40.0% | |
| CATCACGCTAGTAGAATTAG+AGG | - | Chr3:94036511-94036530 | None:intergenic | 40.0% | |
| CTCCTCTAATAGCATCAAGA+GGG | + | Chr3:94036545-94036564 | MsG0380017248.01.T01:CDS | 40.0% | |
| CTCTAACAGAAGAGAACAAG+AGG | + | Chr3:94037673-94037692 | MsG0380017248.01.T01:CDS | 40.0% | |
| CTTGATGCTATTAGAGGAGT+TGG | - | Chr3:94036544-94036563 | None:intergenic | 40.0% | |
| TAGAAGTGAATTTGGGCCTT+TGG | - | Chr3:94037876-94037895 | None:intergenic | 40.0% | |
| ! | CTTGTGCCACTCATAAAGTA+TGG | + | Chr3:94038249-94038268 | MsG0380017248.01.T01:three_prime_UTR | 40.0% |
| !! | ATTTCTCTTGGTCTTGGTCT+TGG | + | Chr3:94036285-94036304 | MsG0380017248.01.T01:CDS | 40.0% |
| AGAAGGGCAAACTGTAAGAC+TGG | - | Chr3:94037778-94037797 | None:intergenic | 45.0% | |
| AGAGAACAAGAGGCTAGAAG+AGG | + | Chr3:94037683-94037702 | MsG0380017248.01.T01:CDS | 45.0% | |
| AGATTAATCTGAGCGTGCGA+AGG | + | Chr3:94037405-94037424 | MsG0380017248.01.T01:intron | 45.0% | |
| AGGTGTGGTTTCAAAACAGG+AGG | + | Chr3:94037220-94037239 | MsG0380017248.01.T01:CDS | 45.0% | |
| ATTAGGGGATCCAACTCCTA+AGG | - | Chr3:94036397-94036416 | None:intergenic | 45.0% | |
| CAAACTGTAAGACTGGCAAC+CGG | - | Chr3:94037771-94037790 | None:intergenic | 45.0% | |
| CTCCCTCTTGATGCTATTAG+AGG | - | Chr3:94036550-94036569 | None:intergenic | 45.0% | |
| CTGGCTCATGATGTACTTCT+TGG | - | Chr3:94036590-94036609 | None:intergenic | 45.0% | |
| GAAGATTAAGGAGGAAGAGG+AGG | + | Chr3:94036338-94036357 | MsG0380017248.01.T01:CDS | 45.0% | |
| GCAACTATTGCTAGCTAGCT+AGG | + | Chr3:94037933-94037952 | MsG0380017248.01.T01:exon | 45.0% | |
| GGTGTGGTTTCAAAACAGGA+GGG | + | Chr3:94037221-94037240 | MsG0380017248.01.T01:CDS | 45.0% | |
| TGGCAAGCAAGCTGAATCTT+CGG | + | Chr3:94037184-94037203 | MsG0380017248.01.T01:CDS | 45.0% | |
| ! | AAAAGAATTTGCACCGGCAC+CGG | + | Chr3:94037801-94037820 | MsG0380017248.01.T01:CDS | 45.0% |
| ! | TGGAGGTGTGGTTTCAAAAC+AGG | + | Chr3:94037217-94037236 | MsG0380017248.01.T01:CDS | 45.0% |
| !!! | AATTGAATAAATTTTTTAAA+TGG | - | Chr3:94036921-94036940 | None:intergenic | 5.0% |
| AATCAAAGCCGTGGTGTGAG+AGG | - | Chr3:94037847-94037866 | None:intergenic | 50.0% | |
| GAATCTTCGGACGAGACAAG+TGG | + | Chr3:94037197-94037216 | MsG0380017248.01.T01:CDS | 50.0% | |
| GGTTTCAAAACAGGAGGGCA+AGG | + | Chr3:94037226-94037245 | MsG0380017248.01.T01:CDS | 50.0% | |
| ! | GGCTTTGATTCTCTGCCCAA+AGG | + | Chr3:94037857-94037876 | MsG0380017248.01.T01:CDS | 50.0% |
| ! | GTCGCTTGCATGTCTAAGAC+TGG | - | Chr3:94037332-94037351 | None:intergenic | 50.0% |
| !! | ACAAACGGAAGTGGACTGTG+AGG | + | Chr3:94037629-94037648 | MsG0380017248.01.T01:CDS | 50.0% |
| !! | GAAGGTGTCCTCCAAGATTG+AGG | - | Chr3:94036691-94036710 | None:intergenic | 50.0% |
| !! | GGGTTGTGCCGTTGTTTTCA+TGG | - | Chr3:94037727-94037746 | None:intergenic | 50.0% |
| !! | TCACGTACGTGCTGCTTATG+TGG | + | Chr3:94038103-94038122 | MsG0380017248.01.T01:three_prime_UTR | 50.0% |
| AGAGGAGCCATTCTCATCGC+CGG | - | Chr3:94037829-94037848 | None:intergenic | 55.0% | |
| CTGAGCGTGCGAAGGATACT+TGG | + | Chr3:94037413-94037432 | MsG0380017248.01.T01:intron | 55.0% | |
| GAATGGCTCCTCTCACACCA+CGG | + | Chr3:94037836-94037855 | MsG0380017248.01.T01:CDS | 55.0% | |
| TCTTCGGACGAGACAAGTGG+AGG | + | Chr3:94037200-94037219 | MsG0380017248.01.T01:CDS | 55.0% | |
| TGGGCAGAGAATCAAAGCCG+TGG | - | Chr3:94037856-94037875 | None:intergenic | 55.0% | |
| GGACGAGACAAGTGGAGGTG+TGG | + | Chr3:94037205-94037224 | MsG0380017248.01.T01:CDS | 60.0% | |
| GCCATTCTCATCGCCGGTGC+CGG | - | Chr3:94037823-94037842 | None:intergenic | 65.0% | |
| !! | ACCGGCACCGGCGATGAGAA+TGG | + | Chr3:94037819-94037838 | MsG0380017248.01.T01:CDS | 65.0% |
| !! | ATTTGCACCGGCACCGGCAC+CGG | + | Chr3:94037807-94037826 | MsG0380017248.01.T01:CDS | 65.0% |
| !! | CTCATCGCCGGTGCCGGTGC+CGG | - | Chr3:94037817-94037836 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 94036221 | 94038325 | 94036221 | ID=MsG0380017248.01;Name=MsG0380017248.01 |
| Chr3 | mRNA | 94036221 | 94038325 | 94036221 | ID=MsG0380017248.01.T01;Parent=MsG0380017248.01;Name=MsG0380017248.01.T01;_AED=0.22;_eAED=0.22;_QI=43|1|1|1|1|1|3|375|294 |
| Chr3 | exon | 94036221 | 94036734 | 94036221 | ID=MsG0380017248.01.T01:exon:17179;Parent=MsG0380017248.01.T01 |
| Chr3 | exon | 94037168 | 94037247 | 94037168 | ID=MsG0380017248.01.T01:exon:17180;Parent=MsG0380017248.01.T01 |
| Chr3 | exon | 94037617 | 94038325 | 94037617 | ID=MsG0380017248.01.T01:exon:17181;Parent=MsG0380017248.01.T01 |
| Chr3 | five_prime_UTR | 94036221 | 94036263 | 94036221 | ID=MsG0380017248.01.T01:five_prime_utr;Parent=MsG0380017248.01.T01 |
| Chr3 | CDS | 94036264 | 94036734 | 94036264 | ID=MsG0380017248.01.T01:cds;Parent=MsG0380017248.01.T01 |
| Chr3 | CDS | 94037168 | 94037247 | 94037168 | ID=MsG0380017248.01.T01:cds;Parent=MsG0380017248.01.T01 |
| Chr3 | CDS | 94037617 | 94037950 | 94037617 | ID=MsG0380017248.01.T01:cds;Parent=MsG0380017248.01.T01 |
| Chr3 | three_prime_UTR | 94037951 | 94038325 | 94037951 | ID=MsG0380017248.01.T01:three_prime_utr;Parent=MsG0380017248.01.T01 |
| Gene Sequence |
| Protein sequence |
