AlfalfaGEDB | MsG0380017553.01

M. sativa cultivar ZhongmuNo.1 / MsG0380017553.01

KEGG: -
GO: GO:0003700 GO:0006355 GO:0043565
PFAM: PF03106 PF10533

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017553.01.T01	XP_003603547.1	93.043	115	4	1	15	129	291	401	9.16E-71	227

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017553.01.T01	Q9SV15	69.072	97	26	1	16	112	224	316	1.55E-42	145

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017553.01.T01	G7J2S9	93.043	115	4	1	15	129	291	401	4.37e-71	227

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TFs/TRs:

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Gene ID	Type	Classification
MsG0380017553.01.T01	TF	WRKY

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017553.01.T01	MTR_3g108960	93.043	115	4	1	15	129	291	401	1.11e-74	227
MsG0380017553.01.T01	MTR_0117s0040	89.024	82	9	0	17	98	53	134	2.55e-49	158
MsG0380017553.01.T01	MTR_1g009613	67.708	96	28	1	15	110	239	331	6.69e-42	141
MsG0380017553.01.T01	MTR_4g127040	69.412	85	26	0	13	97	220	304	2.42e-40	137
MsG0380017553.01.T01	MTR_5g016610	65.476	84	29	0	12	95	173	256	5.68e-36	124
MsG0380017553.01.T01	MTR_4g094908	67.500	80	26	0	16	95	244	323	1.08e-34	122
MsG0380017553.01.T01	MTR_1g070455	59.302	86	35	0	16	101	271	356	5.81e-34	121
MsG0380017553.01.T01	MTR_2g016770	50.000	86	42	1	11	95	7	92	2.45e-26	98.2
MsG0380017553.01.T01	MTR_2g032030	51.163	86	37	1	15	95	239	324	1.81e-25	99.8
MsG0380017553.01.T01	MTR_2g032030	51.163	86	37	1	15	95	239	324	1.95e-25	100

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017553.01.T01	AT4G31550	68.367	98	27	1	15	112	222	315	8.18e-44	146
MsG0380017553.01.T01	AT4G31550	69.072	97	26	1	16	112	224	316	1.58e-43	145
MsG0380017553.01.T01	AT2G24570	64.646	99	31	1	16	114	221	315	5.43e-41	139
MsG0380017553.01.T01	AT2G30590	61.364	88	34	0	15	102	290	377	9.88e-36	126
MsG0380017553.01.T01	AT4G24240	54.737	95	43	0	15	109	258	352	3.42e-35	124
MsG0380017553.01.T01	AT2G23320	60.494	81	32	0	15	95	217	297	1.61e-34	122
MsG0380017553.01.T01	AT3G04670	57.955	88	37	0	11	98	235	322	4.26e-34	121
MsG0380017553.01.T01	AT5G28650	56.818	88	38	0	11	98	235	322	1.69e-33	119
MsG0380017553.01.T01	AT2G34830	52.326	86	36	2	15	95	187	272	1.83e-25	99.8
MsG0380017553.01.T01	AT2G34830	52.326	86	36	2	15	95	196	281	2.00e-25	99.8

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Find 45 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTGTCCAGCTAGGAAA+AGG	0.409042	3:-97813126	MsG0380017553.01.T01:CDS
AAATTGTCCAGCTAGGAAAA+GGG	0.578813	3:-97813125	MsG0380017553.01.T01:CDS
AATAATTCTGGTTCAGGTTC+AGG	0.270724	3:-97813007	MsG0380017553.01.T01:CDS
AATCACTCCGGCGATGTCTC+TGG	0.334104	3:-97813702	None:intergenic
AGAATTATTCTCTTGCATTG+GGG	0.619176	3:+97813021	None:intergenic
AGCTAGGAAAAGGGTGGAGA+AGG	0.508533	3:-97813116	MsG0380017553.01.T01:CDS
AGGGAGTGGGAGCATGGATT+TGG	0.234912	3:-97812987	MsG0380017553.01.T01:CDS
AGGTTCAGGGAGTGGGAGCA+TGG	0.522327	3:-97812993	MsG0380017553.01.T01:CDS
ATAATTCTGGTTCAGGTTCA+GGG	0.485370	3:-97813006	MsG0380017553.01.T01:CDS
ATCACTCCGGCGATGTCTCT+GGG	0.478282	3:-97813701	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAATTATTCTCTTGCATTG+GGG	+	Chr3:97813626-97813645	None:intergenic	30.0%
CAGAATTATTCTCTTGCATT+GGG	+	Chr3:97813627-97813646	None:intergenic	30.0%
TGGTAATAGTCAACATTCAA+TGG	-	Chr3:97813026-97813045	MsG0380017553.01.T01:CDS	30.0%
AAAATTGTCCAGCTAGGAAA+AGG	-	Chr3:97813518-97813537	MsG0380017553.01.T01:intron	35.0%
AAATCAATCATCAGTCACCT+TGG	+	Chr3:97813392-97813411	None:intergenic	35.0%
AAATTGTCCAGCTAGGAAAA+GGG	-	Chr3:97813519-97813538	MsG0380017553.01.T01:intron	35.0%
ATTAATTTACCTTCTGGCAG+TGG	+	Chr3:97812992-97813011	None:intergenic	35.0%
CCAATGCAAGAGAATAATTC+TGG	-	Chr3:97813625-97813644	MsG0380017553.01.T01:intron	35.0%
CCAGAATTATTCTCTTGCAT+TGG	+	Chr3:97813628-97813647	None:intergenic	35.0%
CGTTTGTCGTAAAAAAGACA+AGG	-	Chr3:97813105-97813124	MsG0380017553.01.T01:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	97812955	97813711	97812955	ID=MsG0380017553.01;Name=MsG0380017553.01
Chr3	mRNA	97812955	97813711	97812955	ID=MsG0380017553.01.T01;Parent=MsG0380017553.01;Name=MsG0380017553.01.T01;_AED=0.39;_eAED=0.46;_QI=0\|0\|0\|1\|1\|1\|3\|0\|130
Chr3	exon	97813665	97813711	97813665	ID=MsG0380017553.01.T01:exon:21373;Parent=MsG0380017553.01.T01
Chr3	exon	97813273	97813398	97813273	ID=MsG0380017553.01.T01:exon:21372;Parent=MsG0380017553.01.T01
Chr3	exon	97812955	97813174	97812955	ID=MsG0380017553.01.T01:exon:21371;Parent=MsG0380017553.01.T01
Chr3	CDS	97813665	97813711	97813665	ID=MsG0380017553.01.T01:cds;Parent=MsG0380017553.01.T01
Chr3	CDS	97813273	97813398	97813273	ID=MsG0380017553.01.T01:cds;Parent=MsG0380017553.01.T01
Chr3	CDS	97812955	97813174	97812955	ID=MsG0380017553.01.T01:cds;Parent=MsG0380017553.01.T01

Gene Sequence

>MsG0380017553.01.T01

ATGTCTCTGGGAAAACCTCCGCATCTAAGAAATGCCACTGCCAGAAGGAAAAATCGGAACAGGAGACAAGTGAGAGTCCCGTGCATAAGTTCAAGGATTGCTGATATACCAGCAGATGAGTATTCATGGAGGAAGTATGGCTCCAAACCGATTAAGGGAACACCACATCCAAGGGGTTATTATAGGTGCACTATGTCTAAAAATTGTCCAGCTAGGAAAAGGGTGGAGAAGGCGAAAGATGATCCAAATATACTAGTTGTAACGTATGAGTTTGAGCATCGTCACCACCAGCTTCATAATCAAGCCCCAATGCAAGAGAATAATTCTGGTTCAGGTTCAGGGAGTGGGAGCATGGATTTGGGGGTGTTTGAGCTAACCTTAGGAGGGAATTAG

Protein sequence

>MsG0380017553.01.T01

MSLGKPPHLRNATARRKNRNRRQVRVPCISSRIADIPADEYSWRKYGSKPIKGTPHPRGYYRCTMSKNCPARKRVEKAKDDPNILVVTYEFEHRHHQLHNQAPMQENNSGSGSGSGSMDLGVFELTLGGN*