AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018294.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018294.01.T01 MTR_4g009540 74.359 234 26 4 98 298 31 263 7.63e-98 290
MsG0480018294.01.T01 MTR_4g009540 74.359 234 26 4 98 298 31 263 8.84e-98 291
MsG0480018294.01.T01 MTR_4g009110 80.128 156 22 2 149 298 130 282 9.68e-80 246
MsG0480018294.01.T01 MTR_2g104550 52.778 144 57 3 155 298 112 244 2.99e-34 127
MsG0480018294.01.T01 MTR_2g104490 46.835 158 67 4 144 298 97 240 7.85e-30 115
MsG0480018294.01.T01 MTR_2g104490 46.835 158 67 4 144 298 97 240 8.80e-30 115
MsG0480018294.01.T01 MTR_8g009020 54.331 127 49 4 177 298 133 255 2.29e-29 114
MsG0480018294.01.T01 MTR_2g104590 37.714 175 99 3 124 297 11 176 1.37e-27 107
MsG0480018294.01.T01 MTR_4g097950 43.333 150 76 4 152 298 39 182 2.11e-24 99.4
MsG0480018294.01.T01 MTR_4g097940 43.697 119 58 1 180 298 148 257 1.95e-23 98.2
MsG0480018294.01.T01 MTR_0246s0020 47.059 119 62 1 180 298 191 308 2.07e-23 99.0
MsG0480018294.01.T01 MTR_0246s0020 47.059 119 62 1 180 298 191 308 2.12e-23 99.0
MsG0480018294.01.T01 MTR_2g104560 47.414 116 46 3 177 290 44 146 4.42e-23 95.5
MsG0480018294.01.T01 MTR_8g009010 70.000 70 21 0 177 246 124 193 2.31e-22 95.1
MsG0480018294.01.T01 MTR_6g047570 33.171 205 116 5 106 298 69 264 4.45e-22 94.7
MsG0480018294.01.T01 MTR_5g014600 45.082 122 54 3 187 298 149 267 2.41e-21 92.8
MsG0480018294.01.T01 MTR_5g014560 46.154 117 47 3 187 298 131 236 5.13e-21 91.3
MsG0480018294.01.T01 MTR_0250s0040 41.739 115 55 3 187 298 88 193 8.60e-21 89.7
MsG0480018294.01.T01 MTR_2g010450 46.154 117 56 3 183 298 173 283 2.44e-20 90.1
MsG0480018294.01.T01 MTR_4g097920 40.164 122 58 2 180 298 133 242 8.29e-20 88.2
MsG0480018294.01.T01 MTR_4g097920 40.164 122 58 2 180 298 148 257 1.01e-19 88.2
MsG0480018294.01.T01 MTR_2g104530 42.975 121 55 2 180 298 98 206 1.13e-19 87.0
MsG0480018294.01.T01 MTR_8g069740 43.902 123 64 2 177 298 102 220 1.21e-19 86.7
MsG0480018294.01.T01 MTR_5g014520 41.538 130 68 2 169 298 141 262 1.32e-19 87.8
MsG0480018294.01.T01 MTR_4g092700 44.643 112 56 2 187 298 158 263 2.39e-19 87.0
MsG0480018294.01.T01 MTR_0011s0260 40.870 115 56 2 187 298 183 288 6.48e-19 86.3
MsG0480018294.01.T01 MTR_7g080780 53.165 79 36 1 175 252 139 217 9.53e-19 85.1
MsG0480018294.01.T01 MTR_7g080780 52.500 80 37 1 175 253 152 231 9.84e-19 85.5
MsG0480018294.01.T01 MTR_0011s0210 40.000 115 57 2 187 298 183 288 2.23e-18 84.7
MsG0480018294.01.T01 MTR_5g014640 42.969 128 58 3 174 298 141 256 5.60e-18 83.2
MsG0480018294.01.T01 MTR_4g067010 35.849 159 84 5 147 298 102 249 2.16e-17 81.6
MsG0480018294.01.T01 MTR_2g104500 38.095 147 72 4 153 298 76 204 6.59e-17 79.3
MsG0480018294.01.T01 MTR_6g047550 33.557 149 88 3 116 254 93 240 2.68e-16 77.4
MsG0480018294.01.T01 MTR_0246s0050 41.667 120 55 3 180 298 144 249 2.72e-16 78.2
MsG0480018294.01.T01 MTR_4g098035 41.667 120 55 3 180 298 165 270 3.99e-16 77.8
MsG0480018294.01.T01 MTR_2g104650 35.838 173 91 5 133 298 43 202 4.26e-15 74.3
MsG0480018294.01.T01 MTR_4g066460 37.705 122 67 2 177 298 145 257 1.78e-14 73.2
MsG0480018294.01.T01 MTR_8g024790 31.013 158 83 7 149 298 258 397 1.08e-13 71.6
MsG0480018294.01.T01 MTR_4g066380 42.353 85 49 0 158 242 124 208 3.64e-13 69.3
MsG0480018294.01.T01 MTR_7g053420 52.174 69 29 2 13 77 243 311 1.00e-12 67.8
MsG0480018294.01.T01 MTR_8g024770 29.134 127 82 3 177 298 265 388 1.65e-12 67.8
MsG0480018294.01.T01 MTR_1g072320 43.333 60 34 0 187 246 470 529 2.23e-12 67.8
MsG0480018294.01.T01 MTR_8g098275 36.250 80 49 1 171 248 226 305 1.17e-11 65.5
MsG0480018294.01.T01 MTR_8g098275 36.250 80 49 1 171 248 383 462 1.37e-11 65.5
MsG0480018294.01.T01 MTR_8g027495 40.625 64 38 0 186 249 457 520 1.45e-11 65.1
MsG0480018294.01.T01 MTR_8g098275 41.270 63 37 0 186 248 307 369 1.46e-11 65.1
MsG0480018294.01.T01 MTR_8g067280 40.506 79 43 1 175 249 450 528 1.87e-11 65.1
MsG0480018294.01.T01 MTR_5g030430 41.333 75 37 1 175 242 481 555 7.81e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018294.01.T01 AT4G37850 42.149 121 64 2 179 298 144 259 2.75e-21 92.4
MsG0480018294.01.T01 AT4G37850 42.149 121 64 2 179 298 144 259 2.75e-21 92.4
MsG0480018294.01.T01 AT2G22750 40.876 137 80 1 163 299 102 237 2.12e-19 87.0
MsG0480018294.01.T01 AT2G22750 40.876 137 80 1 163 299 102 237 2.15e-19 86.7
MsG0480018294.01.T01 AT2G22750 41.176 136 79 1 163 298 130 264 2.20e-19 87.0
MsG0480018294.01.T01 AT2G22750 41.176 136 79 1 163 298 130 264 2.20e-19 87.0
MsG0480018294.01.T01 AT2G22750 41.176 136 79 1 163 298 126 260 2.34e-19 87.0
MsG0480018294.01.T01 AT2G22760 41.176 119 67 1 180 298 112 227 4.41e-18 82.8
MsG0480018294.01.T01 AT2G22760 41.176 119 67 1 180 298 112 227 4.81e-18 82.8
MsG0480018294.01.T01 AT2G22770 60.714 56 22 0 187 242 132 187 2.85e-16 78.2
MsG0480018294.01.T01 AT2G46510 41.892 74 41 1 175 248 383 454 5.91e-12 66.6
MsG0480018294.01.T01 AT4G09820 44.776 67 37 0 174 240 350 416 1.49e-11 65.1
MsG0480018294.01.T01 AT4G00870 40.000 65 39 0 185 249 247 311 2.00e-11 64.7

Find 59 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTACGTTTATTCTTAGAATT+AGG 0.132709 4:+2948326 None:intergenic
CAATCTCTGGAAGGATATTA+TGG 0.246345 4:+2947802 None:intergenic
TTCCACATGAATAAGGTTTC+CGG 0.268284 4:+2947883 None:intergenic
CCGAGTTGGAATTAGCAAAA+TGG 0.311205 4:-2949804 MsG0480018294.01.T01:CDS
ATATAATGGCAGAGAGAAAA+AGG 0.314718 4:-2948214 MsG0480018294.01.T01:CDS
TACGTTTATTCTTAGAATTA+GGG 0.324348 4:+2948327 None:intergenic
GCACTTTCAGCTACTATTCC+TGG 0.324686 4:-2948164 MsG0480018294.01.T01:CDS
TTATTGTTTCCACATGAATA+AGG 0.328153 4:+2947876 None:intergenic
AACAATTCTGTTGCCGTTAA+CGG 0.337331 4:-2948532 MsG0480018294.01.T01:CDS
AACGGTGATGTTAAGGTTGT+TGG 0.342281 4:-2948514 MsG0480018294.01.T01:CDS
AACCTTAACATCACCGTTAA+CGG 0.356032 4:+2948519 None:intergenic
GTACGATTGTCTTGTGTTGC+AGG 0.364516 4:-2950766 MsG0480018294.01.T01:CDS
GTATCAGAGATGTGTAAGTT+AGG 0.373113 4:-2950864 None:intergenic
ATTTGTTTCACTTAAATTTG+TGG 0.385694 4:+2947842 None:intergenic
GTGACTCTTGCTTCAATCTC+TGG 0.387034 4:+2947789 None:intergenic
AGGTTATTTGCGTAGGAGGA+AGG 0.392179 4:-2950805 MsG0480018294.01.T01:CDS
TAAGTTAGGGTCGGGGTTGG+AGG 0.423339 4:-2950850 MsG0480018294.01.T01:CDS
ACAATGTATTGATCATATAA+TGG 0.436092 4:-2948228 MsG0480018294.01.T01:CDS
GAGATTGAAGCAAGAGTCAC+TGG 0.449490 4:-2947786 MsG0480018294.01.T01:CDS
CGAGTTGGAATTAGCAAAAT+GGG 0.452865 4:-2949803 MsG0480018294.01.T01:CDS
ATCAAATCCGTCATTGAGAT+TGG 0.456871 4:+2950653 None:intergenic
GAGATGTGTAAGTTAGGGTC+GGG 0.459818 4:-2950858 MsG0480018294.01.T01:CDS
ACGGTGATGTTAAGGTTGTT+GGG 0.463224 4:-2948513 MsG0480018294.01.T01:CDS
TGCCGTTAACGGTGATGTTA+AGG 0.465008 4:-2948521 MsG0480018294.01.T01:CDS
CAATTTACCTTGCTCAAGCC+AGG 0.465848 4:+2948146 None:intergenic
GGTTTCCGGAGAACCATTAC+CGG 0.470726 4:+2947897 None:intergenic
AAAATAAATCAACAAATGAA+TGG 0.475843 4:-2948275 MsG0480018294.01.T01:CDS
TGTTGGTGTCACGCCGGTAA+TGG 0.479445 4:-2947910 MsG0480018294.01.T01:CDS
TTGCTAATTCCAACTCGGGT+TGG 0.495414 4:+2949809 None:intergenic
TAGGGTCGGGGTTGGAGGTG+AGG 0.500698 4:-2950845 MsG0480018294.01.T01:CDS
CTATTTAATCGTAGGAGTAA+AGG 0.501712 4:-2949835 MsG0480018294.01.T01:intron
TGCAGGTTGGAACGATTGAC+AGG 0.510842 4:-2950749 MsG0480018294.01.T01:CDS
GTGTAAGTTAGGGTCGGGGT+TGG 0.516367 4:-2950853 MsG0480018294.01.T01:CDS
GGTTATTTGCGTAGGAGGAA+GGG 0.519818 4:-2950804 MsG0480018294.01.T01:CDS
TATCAGAGATGTGTAAGTTA+GGG 0.522278 4:-2950863 None:intergenic
CAAATGAATGGAGTGAAGAA+AGG 0.526653 4:-2948263 MsG0480018294.01.T01:CDS
ATCAGCATATTCTTATCTAA+CGG 0.532956 4:-2950685 MsG0480018294.01.T01:CDS
TCACGCCGGTAATGGTTCTC+CGG 0.534476 4:-2947902 MsG0480018294.01.T01:CDS
CTGTGTAACACTGCGACACA+TGG 0.535796 4:+2950712 None:intergenic
GAACGATTGACAGGTGGATG+TGG 0.536753 4:-2950740 MsG0480018294.01.T01:CDS
TAAAGGAAACCAACCCGAGT+TGG 0.550732 4:-2949818 MsG0480018294.01.T01:CDS
GAGGAGGTTATTTGCGTAGG+AGG 0.560231 4:-2950809 MsG0480018294.01.T01:CDS
ACGGAGGAGGTTATTTGCGT+AGG 0.560315 4:-2950812 MsG0480018294.01.T01:CDS
GATTGTCTTGTGTTGCAGGT+TGG 0.566051 4:-2950762 MsG0480018294.01.T01:CDS
CTCCGGAAACCTTATTCATG+TGG 0.574739 4:-2947885 MsG0480018294.01.T01:CDS
TGAAGCAAGAGTCACTGGAA+AGG 0.583766 4:-2947781 MsG0480018294.01.T01:CDS
TACTATTCCTGGCTTGAGCA+AGG 0.585542 4:-2948153 MsG0480018294.01.T01:intron
AGAGATGTGTAAGTTAGGGT+CGG 0.585776 4:-2950859 None:intergenic
CTCTTGCTTCAATCTCTGGA+AGG 0.599610 4:+2947793 None:intergenic
AGGCGTGAAAATGACGGAGG+AGG 0.611564 4:-2950825 MsG0480018294.01.T01:CDS
TATGAACTTGGTTTATCATG+TGG 0.621367 4:+2949763 None:intergenic
AGATGTGTAAGTTAGGGTCG+GGG 0.626707 4:-2950857 MsG0480018294.01.T01:CDS
ATTGATACCAATCTCAATGA+CGG 0.633469 4:-2950660 MsG0480018294.01.T01:CDS
ACGTTTATTCTTAGAATTAG+GGG 0.633884 4:+2948328 None:intergenic
CAAGAACAAGAACAACAACA+TGG 0.670092 4:-2948436 MsG0480018294.01.T01:intron
GAGGTGAGGCGTGAAAATGA+CGG 0.676335 4:-2950831 MsG0480018294.01.T01:CDS
GTAGGAGGAAGGGTTACTCA+TGG 0.681730 4:-2950794 MsG0480018294.01.T01:CDS
GTGAGGCGTGAAAATGACGG+AGG 0.708458 4:-2950828 MsG0480018294.01.T01:CDS
AGGTTGGAACGATTGACAGG+TGG 0.743279 4:-2950746 MsG0480018294.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAATTTACTTTTAAAACA+TGG - Chr4:2949463-2949482 MsG0480018294.01.T01:intron 10.0%
!!! TATTAAATAATTAGTTTCAT+CGG - Chr4:2950535-2950554 MsG0480018294.01.T01:intron 10.0%
!! AAAAAATAATGAATTGTTGT+AGG - Chr4:2949970-2949989 MsG0480018294.01.T01:intron 15.0%
!! AAAAACAAAATATATGTCTT+TGG + Chr4:2950248-2950267 None:intergenic 15.0%
!! AAAATAAATCAACAAATGAA+TGG - Chr4:2950347-2950366 MsG0480018294.01.T01:intron 15.0%
!! ACTAATTATTTAATATGAGA+TGG + Chr4:2950530-2950549 None:intergenic 15.0%
!! TACTAATTATTATAGTGAAA+CGG + Chr4:2949125-2949144 None:intergenic 15.0%
!! AAAATAGAGTAAATGACTAA+GGG + Chr4:2949737-2949756 None:intergenic 20.0%
!! AAACTGAATTATCATAACTT+TGG + Chr4:2949564-2949583 None:intergenic 20.0%
!! AATAAAACCTATTTAATCGT+AGG - Chr4:2948779-2948798 MsG0480018294.01.T01:intron 20.0%
!! AATTATTTAATATGAGATGG+AGG + Chr4:2950527-2950546 None:intergenic 20.0%
!! ATTTGTTTCACTTAAATTTG+TGG + Chr4:2950783-2950802 None:intergenic 20.0%
!! TAAAATAGAGTAAATGACTA+AGG + Chr4:2949738-2949757 None:intergenic 20.0%
!! TACGTTTATTCTTAGAATTA+GGG + Chr4:2950298-2950317 None:intergenic 20.0%
!! TGTCACACTATATATAATTT+GGG - Chr4:2949943-2949962 MsG0480018294.01.T01:intron 20.0%
!! TTGTCACACTATATATAATT+TGG - Chr4:2949942-2949961 MsG0480018294.01.T01:intron 20.0%
!! TTTACAAAAGAGATATCAAT+GGG - Chr4:2949147-2949166 MsG0480018294.01.T01:intron 20.0%
!!! ACAATGTATTGATCATATAA+TGG - Chr4:2950394-2950413 MsG0480018294.01.T01:intron 20.0%
!!! ATTTCGGTACTTTTTTTATA+TGG - Chr4:2948315-2948334 MsG0480018294.01.T01:CDS 20.0%
!!! TTTTTTTTCCTTTTTTTGAC+TGG - Chr4:2948514-2948533 MsG0480018294.01.T01:CDS 20.0%
! AATTATTATAGTGAAACGGA+AGG + Chr4:2949121-2949140 None:intergenic 25.0%
! ACACTAATCATTTGTTCTTT+CGG - Chr4:2948145-2948164 MsG0480018294.01.T01:intron 25.0%
! ACGTTTATTCTTAGAATTAG+GGG + Chr4:2950297-2950316 None:intergenic 25.0%
! ATCAGCATATTCTTATCTAA+CGG - Chr4:2947937-2947956 MsG0480018294.01.T01:CDS 25.0%
! CCATAAAATTGTAACAAATC+TGG - Chr4:2949406-2949425 MsG0480018294.01.T01:intron 25.0%
! CTACGTTTATTCTTAGAATT+AGG + Chr4:2950299-2950318 None:intergenic 25.0%
! CTTTACAAAAGAGATATCAA+TGG - Chr4:2949146-2949165 MsG0480018294.01.T01:intron 25.0%
! TTATTGTTTCCACATGAATA+AGG + Chr4:2950749-2950768 None:intergenic 25.0%
! TTCTAATCATGATATGAACT+TGG + Chr4:2948874-2948893 None:intergenic 25.0%
! TTCTGAATAGATTAAACTTG+TGG + Chr4:2950064-2950083 None:intergenic 25.0%
!! ACATGGTTTGAAATCAAAAA+AGG - Chr4:2950203-2950222 MsG0480018294.01.T01:intron 25.0%
!!! AAAGTACCGAAATCAAATAA+CGG + Chr4:2948308-2948327 None:intergenic 25.0%
!!! CCAGATTTGTTACAATTTTA+TGG + Chr4:2949409-2949428 None:intergenic 25.0%
!!! TTTTTAGGCAAAGATACAAA+CGG + Chr4:2949528-2949547 None:intergenic 25.0%
ATATAATGGCAGAGAGAAAA+AGG - Chr4:2950408-2950427 MsG0480018294.01.T01:intron 30.0%
ATTGATACCAATCTCAATGA+CGG - Chr4:2947962-2947981 MsG0480018294.01.T01:CDS 30.0%
CTATTTAATCGTAGGAGTAA+AGG - Chr4:2948787-2948806 MsG0480018294.01.T01:intron 30.0%
CTTTACTCCTACGATTAAAT+AGG + Chr4:2948789-2948808 None:intergenic 30.0%
GAGAAACAAGACAAAAATGT+TGG - Chr4:2950695-2950714 MsG0480018294.01.T01:CDS 30.0%
GTTCATATCATGATTAGAAG+AGG - Chr4:2948875-2948894 MsG0480018294.01.T01:intron 30.0%
TAACTTCCATACACTTTGTT+TGG + Chr4:2948991-2949010 None:intergenic 30.0%
TAGGAAGCCTATTTAAAAAG+AGG + Chr4:2948047-2948066 None:intergenic 30.0%
TATGAACTTGGTTTATCATG+TGG + Chr4:2948862-2948881 None:intergenic 30.0%
TGAATAGATTAAACTTGTGG+TGG + Chr4:2950061-2950080 None:intergenic 30.0%
TTGTATAGAGTTTGTCTCTT+GGG + Chr4:2949290-2949309 None:intergenic 30.0%
TTGTTGTAGGAATTGAATTG+CGG - Chr4:2949983-2950002 MsG0480018294.01.T01:intron 30.0%
TTTGTATAGAGTTTGTCTCT+TGG + Chr4:2949291-2949310 None:intergenic 30.0%
! AAGAGAAGATATTGGAGTTT+TGG + Chr4:2949203-2949222 None:intergenic 30.0%
! AATTTGGTTTCAAAGTGGTT+GGG - Chr4:2948191-2948210 MsG0480018294.01.T01:CDS 30.0%
! AGAGAAGATATTGGAGTTTT+GGG + Chr4:2949202-2949221 None:intergenic 30.0%
! CGTTTACCGTTATTTGATTT+CGG - Chr4:2948299-2948318 MsG0480018294.01.T01:CDS 30.0%
! GGAAAATTTTCATCTTGACA+AGG - Chr4:2949865-2949884 MsG0480018294.01.T01:intron 30.0%
! TATGACGCCTCTTTTTAAAT+AGG - Chr4:2948037-2948056 MsG0480018294.01.T01:intron 30.0%
! TATGGAATTTGGTTTCAAAG+TGG - Chr4:2948186-2948205 MsG0480018294.01.T01:CDS 30.0%
! TTTATGTCGGTTATGGAATT+TGG - Chr4:2948175-2948194 MsG0480018294.01.T01:CDS 30.0%
!! ATGTGTGAATGATTAGTTTG+AGG + Chr4:2949604-2949623 None:intergenic 30.0%
!! TCCATTTTTATAGACAGACA+AGG - Chr4:2950610-2950629 MsG0480018294.01.T01:intron 30.0%
!!! AACGTATTCCTAGAGTTTTT+TGG + Chr4:2948283-2948302 None:intergenic 30.0%
!!! TATAGTCGTGACCTTTTTTA+AGG - Chr4:2948693-2948712 MsG0480018294.01.T01:intron 30.0%
!!! TTCGATTTTGATTACTCTGA+TGG - Chr4:2948445-2948464 MsG0480018294.01.T01:CDS 30.0%
!!! TTGTTTTAGCACGTGTTTTT+GGG + Chr4:2949432-2949451 None:intergenic 30.0%
!!! TTTGTTTTAGCACGTGTTTT+TGG + Chr4:2949433-2949452 None:intergenic 30.0%
AACAATTCTGTTGCCGTTAA+CGG - Chr4:2950090-2950109 MsG0480018294.01.T01:intron 35.0%
AACCACAACCAGTCAAAAAA+AGG + Chr4:2948525-2948544 None:intergenic 35.0%
AACCTTAACATCACCGTTAA+CGG + Chr4:2950106-2950125 None:intergenic 35.0%
AAGCAACCAAACAAAGTGTA+TGG - Chr4:2948982-2949001 MsG0480018294.01.T01:intron 35.0%
AAGGAAAATCTACTTAGGAG+AGG - Chr4:2948069-2948088 MsG0480018294.01.T01:intron 35.0%
AATCCTAATAGTGTTTCCCA+AGG - Chr4:2949236-2949255 MsG0480018294.01.T01:intron 35.0%
AGAAAGGCAACAACAAAACT+GGG + Chr4:2949314-2949333 None:intergenic 35.0%
AGTGTAAACCTTGAGATAGA+GGG + Chr4:2949354-2949373 None:intergenic 35.0%
AGTGTGGTTAGGAAATTGAT+AGG + Chr4:2949704-2949723 None:intergenic 35.0%
ATCAAATCCGTCATTGAGAT+TGG + Chr4:2947972-2947991 None:intergenic 35.0%
CAAATGAATGGAGTGAAGAA+AGG - Chr4:2950359-2950378 MsG0480018294.01.T01:intron 35.0%
CAAGAACAAGAACAACAACA+TGG - Chr4:2950186-2950205 MsG0480018294.01.T01:intron 35.0%
CACCAATAATACTTGATGTG+TGG - Chr4:2948945-2948964 MsG0480018294.01.T01:intron 35.0%
CGGTGATGATTATTTCATAG+GGG - Chr4:2950003-2950022 MsG0480018294.01.T01:intron 35.0%
CTACCAAGGAAAATCTACTT+AGG - Chr4:2948064-2948083 MsG0480018294.01.T01:intron 35.0%
CTCCACACATCAAGTATTAT+TGG + Chr4:2948950-2948969 None:intergenic 35.0%
GCCTTGTCTGTCTATAAAAA+TGG + Chr4:2950614-2950633 None:intergenic 35.0%
GCGGTGATGATTATTTCATA+GGG - Chr4:2950002-2950021 MsG0480018294.01.T01:intron 35.0%
GGATGTTTCCAAAAAACTCT+AGG - Chr4:2948272-2948291 MsG0480018294.01.T01:CDS 35.0%
GTACTACAATAAGTGTGGTT+AGG + Chr4:2949715-2949734 None:intergenic 35.0%
GTGTGGTTAGGAAATTGATA+GGG + Chr4:2949703-2949722 None:intergenic 35.0%
TAGAAAGGCAACAACAAAAC+TGG + Chr4:2949315-2949334 None:intergenic 35.0%
TCTTGGTCAAGAGAAGATAT+TGG + Chr4:2949211-2949230 None:intergenic 35.0%
TGCGGTGATGATTATTTCAT+AGG - Chr4:2950001-2950020 MsG0480018294.01.T01:intron 35.0%
TGGGAAACACTATTAGGATT+GGG + Chr4:2949236-2949255 None:intergenic 35.0%
TTCCACATGAATAAGGTTTC+CGG + Chr4:2950742-2950761 None:intergenic 35.0%
TTGCCTAAAAACAGATAGCA+GGG - Chr4:2949537-2949556 MsG0480018294.01.T01:intron 35.0%
TTGGGAAACACTATTAGGAT+TGG + Chr4:2949237-2949256 None:intergenic 35.0%
TTTAAATAGGCTTCCTACCA+AGG - Chr4:2948050-2948069 MsG0480018294.01.T01:intron 35.0%
TTTGCCTAAAAACAGATAGC+AGG - Chr4:2949536-2949555 MsG0480018294.01.T01:intron 35.0%
! AACGATACAGTGTTGGATTT+GGG + Chr4:2949630-2949649 None:intergenic 35.0%
! ATAGCTTTTGTGGCTTAAAG+CGG - Chr4:2947990-2948009 MsG0480018294.01.T01:CDS 35.0%
! ATTTGGTTTCAAAGTGGTTG+GGG - Chr4:2948192-2948211 MsG0480018294.01.T01:CDS 35.0%
! ATTTTCCAATGGAAGAGTCA+TGG - Chr4:2949843-2949862 MsG0480018294.01.T01:intron 35.0%
! CAATCTCTGGAAGGATATTA+TGG + Chr4:2950823-2950842 None:intergenic 35.0%
! CATTTTGCTAATTCCAACTC+GGG + Chr4:2948820-2948839 None:intergenic 35.0%
! CCATTTTGCTAATTCCAACT+CGG + Chr4:2948821-2948840 None:intergenic 35.0%
! CGAGTTGGAATTAGCAAAAT+GGG - Chr4:2948819-2948838 MsG0480018294.01.T01:intron 35.0%
! CTAAGTAGATTTTCCTTGGT+AGG + Chr4:2948066-2948085 None:intergenic 35.0%
! GAATTTGGTTTCAAAGTGGT+TGG - Chr4:2948190-2948209 MsG0480018294.01.T01:CDS 35.0%
! GCTCACATTCTATTTTCCAA+TGG - Chr4:2949832-2949851 MsG0480018294.01.T01:intron 35.0%
! TCTCCTAAGTAGATTTTCCT+TGG + Chr4:2948070-2948089 None:intergenic 35.0%
! TTTGAAGGTTGATTCACCTT+GGG + Chr4:2949255-2949274 None:intergenic 35.0%
! TTTGATTTCAACCGTTGGAT+TGG + Chr4:2949653-2949672 None:intergenic 35.0%
! TTTTCCAATGGAAGAGTCAT+GGG - Chr4:2949844-2949863 MsG0480018294.01.T01:intron 35.0%
!! AAGAATTTGTTGACCCAGTT+CGG - Chr4:2949062-2949081 MsG0480018294.01.T01:intron 35.0%
!! CACTCTTTTGAGTACTTTCA+CGG + Chr4:2950322-2950341 None:intergenic 35.0%
!! CTTTGGAGAAAACTTTTACG+AGG + Chr4:2950231-2950250 None:intergenic 35.0%
!! TAAGGGTACTACAATAAGTG+TGG + Chr4:2949720-2949739 None:intergenic 35.0%
!!! GTCATTTTGATTTCAACCGT+TGG + Chr4:2949658-2949677 None:intergenic 35.0%
!!! TTCCTTTTTTTGACTGGTTG+TGG - Chr4:2948520-2948539 MsG0480018294.01.T01:CDS 35.0%
!!! TTTTTTTGACTGGTTGTGGT+TGG - Chr4:2948524-2948543 MsG0480018294.01.T01:CDS 35.0%
AACGGTGATGTTAAGGTTGT+TGG - Chr4:2950108-2950127 MsG0480018294.01.T01:intron 40.0%
AAGGACTTTCCACAACAAGT+CGG - Chr4:2948352-2948371 MsG0480018294.01.T01:intron 40.0%
AATACTTGATGTGTGGAGCA+AGG - Chr4:2948952-2948971 MsG0480018294.01.T01:intron 40.0%
AATCATGCTCATCAGTTTCG+TGG - Chr4:2948245-2948264 MsG0480018294.01.T01:CDS 40.0%
ACGGTGATGTTAAGGTTGTT+GGG - Chr4:2950109-2950128 MsG0480018294.01.T01:intron 40.0%
ACTATTAGGATTGGGTCTCT+TGG + Chr4:2949228-2949247 None:intergenic 40.0%
ACTGTATCGTTCCAATCCAA+CGG - Chr4:2949639-2949658 MsG0480018294.01.T01:intron 40.0%
ATACTTGATGTGTGGAGCAA+GGG - Chr4:2948953-2948972 MsG0480018294.01.T01:intron 40.0%
CAGTGTAAACCTTGAGATAG+AGG + Chr4:2949355-2949374 None:intergenic 40.0%
CTTAAACACACTTGTGCTTG+AGG - Chr4:2948577-2948596 MsG0480018294.01.T01:intron 40.0%
GTCCAAAATTGACCTAGTCT+GGG - Chr4:2949084-2949103 MsG0480018294.01.T01:intron 40.0%
GTGTAAACCTTGAGATAGAG+GGG + Chr4:2949353-2949372 None:intergenic 40.0%
TATGGAAAGATCGCAACAGA+AGG - Chr4:2948333-2948352 MsG0480018294.01.T01:CDS 40.0%
TCACCTTGGGAAACACTATT+AGG + Chr4:2949242-2949261 None:intergenic 40.0%
TCCAAAATTGACCTAGTCTG+GGG - Chr4:2949085-2949104 MsG0480018294.01.T01:intron 40.0%
TGTAAACCTTGAGATAGAGG+GGG + Chr4:2949352-2949371 None:intergenic 40.0%
TGTCTCTTGGGAACAAACTA+GGG + Chr4:2949278-2949297 None:intergenic 40.0%
TTAGGCAAAGATACAAACGG+TGG + Chr4:2949525-2949544 None:intergenic 40.0%
TTCTTAGAATTAGGGGTAGC+TGG + Chr4:2950290-2950309 None:intergenic 40.0%
TTGTCTCTTGGGAACAAACT+AGG + Chr4:2949279-2949298 None:intergenic 40.0%
! CCGAGTTGGAATTAGCAAAA+TGG - Chr4:2948818-2948837 MsG0480018294.01.T01:intron 40.0%
! GAACGATACAGTGTTGGATT+TGG + Chr4:2949631-2949650 None:intergenic 40.0%
! GTTTGAAGGTTGATTCACCT+TGG + Chr4:2949256-2949275 None:intergenic 40.0%
! TGTGACGTTTATGTCGGTTA+TGG - Chr4:2948168-2948187 MsG0480018294.01.T01:CDS 40.0%
! TTTCGGTGTGACGTTTATGT+CGG - Chr4:2948162-2948181 MsG0480018294.01.T01:CDS 40.0%
! TTTTCCCATGACTCTTCCAT+TGG + Chr4:2949851-2949870 None:intergenic 40.0%
!! GACGGATTTGATAGCTTTTG+TGG - Chr4:2947980-2947999 MsG0480018294.01.T01:CDS 40.0%
AAGCATGATCGATCTTGTGC+AGG + Chr4:2948743-2948762 None:intergenic 45.0%
ACTCCAGAGGGCCTTAAAAA+AGG + Chr4:2948707-2948726 None:intergenic 45.0%
AGGTTATTTGCGTAGGAGGA+AGG - Chr4:2947817-2947836 MsG0480018294.01.T01:CDS 45.0%
CAATTTACCTTGCTCAAGCC+AGG + Chr4:2950479-2950498 None:intergenic 45.0%
CCAAAATTGACCTAGTCTGG+GGG - Chr4:2949086-2949105 MsG0480018294.01.T01:intron 45.0%
CTCCGGAAACCTTATTCATG+TGG - Chr4:2950737-2950756 MsG0480018294.01.T01:CDS 45.0%
CTCTTGCTTCAATCTCTGGA+AGG + Chr4:2950832-2950851 None:intergenic 45.0%
GAGATTGAAGCAAGAGTCAC+TGG - Chr4:2950836-2950855 MsG0480018294.01.T01:CDS 45.0%
GATCTTGTGCAGGTGCAATA+AGG + Chr4:2948733-2948752 None:intergenic 45.0%
GATTGTCTTGTGTTGCAGGT+TGG - Chr4:2947860-2947879 MsG0480018294.01.T01:CDS 45.0%
GCACTTTCAGCTACTATTCC+TGG - Chr4:2950458-2950477 MsG0480018294.01.T01:intron 45.0%
GCTGAAACAAGGGCTTAGAA+AGG + Chr4:2949330-2949349 None:intergenic 45.0%
GCTTAAAGCGGTTCTGTTGA+AGG - Chr4:2948002-2948021 MsG0480018294.01.T01:CDS 45.0%
GGATTGGAACGATACAGTGT+TGG + Chr4:2949637-2949656 None:intergenic 45.0%
GGGAACAAACTAGGGTTTGA+AGG + Chr4:2949270-2949289 None:intergenic 45.0%
GGGTTCGTTTCAGTTCTTCA+TGG - Chr4:2948212-2948231 MsG0480018294.01.T01:CDS 45.0%
GGTCCAAAATTGACCTAGTC+TGG - Chr4:2949083-2949102 MsG0480018294.01.T01:intron 45.0%
GGTTATTTGCGTAGGAGGAA+GGG - Chr4:2947818-2947837 MsG0480018294.01.T01:CDS 45.0%
GTACGATTGTCTTGTGTTGC+AGG - Chr4:2947856-2947875 MsG0480018294.01.T01:CDS 45.0%
GTCTTCTGATTCTCCACACA+GGG + Chr4:2948127-2948146 None:intergenic 45.0%
GTGACTCTTGCTTCAATCTC+TGG + Chr4:2950836-2950855 None:intergenic 45.0%
TAAAGGAAACCAACCCGAGT+TGG - Chr4:2948804-2948823 MsG0480018294.01.T01:intron 45.0%
TGAAGCAAGAGTCACTGGAA+AGG - Chr4:2950841-2950860 MsG0480018294.01.T01:CDS 45.0%
TGCCGTTAACGGTGATGTTA+AGG - Chr4:2950101-2950120 MsG0480018294.01.T01:intron 45.0%
TGTCTTCTGATTCTCCACAC+AGG + Chr4:2948128-2948147 None:intergenic 45.0%
TTGCTAATTCCAACTCGGGT+TGG + Chr4:2948816-2948835 None:intergenic 45.0%
! AGGTCAATTTTGGACCGAAC+TGG + Chr4:2949079-2949098 None:intergenic 45.0%
! CTGGCTTGAGCAAGGTAAAT+TGG - Chr4:2950477-2950496 MsG0480018294.01.T01:intron 45.0%
! CTTTTTGCAAGTGTCTCGAG+CGG + Chr4:2948380-2948399 None:intergenic 45.0%
! GGTCAATTTTGGACCGAACT+GGG + Chr4:2949078-2949097 None:intergenic 45.0%
! TACTATTCCTGGCTTGAGCA+AGG - Chr4:2950469-2950488 MsG0480018294.01.T01:intron 45.0%
! TTTTTGCAAGTGTCTCGAGC+GGG + Chr4:2948379-2948398 None:intergenic 45.0%
!! CAAAAATGTTGGTGTCACGC+CGG - Chr4:2950706-2950725 MsG0480018294.01.T01:CDS 45.0%
!! GGACCCTGCTATCTGTTTTT+AGG + Chr4:2949543-2949562 None:intergenic 45.0%
!!! TGACCTTTTTTAAGGCCCTC+TGG - Chr4:2948701-2948720 MsG0480018294.01.T01:intron 45.0%
!!! TTTTTTAAGGCCCTCTGGAG+TGG - Chr4:2948706-2948725 MsG0480018294.01.T01:intron 45.0%
!! AAAAAAAAAATATAATCTTT+AGG - Chr4:2948636-2948655 MsG0480018294.01.T01:intron 5.0%
!! AAAAAAAAATATAATCTTTA+GGG - Chr4:2948637-2948656 MsG0480018294.01.T01:intron 5.0%
ACGGAGGAGGTTATTTGCGT+AGG - Chr4:2947810-2947829 MsG0480018294.01.T01:CDS 50.0%
AGATAGAGGGGGCTGAAACA+AGG + Chr4:2949341-2949360 None:intergenic 50.0%
AGCTACTCAGCTTCCTTGTG+TGG - Chr4:2948101-2948120 MsG0480018294.01.T01:intron 50.0%
AGGTTGGAACGATTGACAGG+TGG - Chr4:2947876-2947895 MsG0480018294.01.T01:CDS 50.0%
CGCTCGAGACACTTGCAAAA+AGG - Chr4:2948378-2948397 MsG0480018294.01.T01:intron 50.0%
CTGTGTAACACTGCGACACA+TGG + Chr4:2947913-2947932 None:intergenic 50.0%
GAACGATTGACAGGTGGATG+TGG - Chr4:2947882-2947901 MsG0480018294.01.T01:CDS 50.0%
GAGGAGGTTATTTGCGTAGG+AGG - Chr4:2947813-2947832 MsG0480018294.01.T01:CDS 50.0%
GAGGTGAGGCGTGAAAATGA+CGG - Chr4:2947791-2947810 MsG0480018294.01.T01:CDS 50.0%
GATAGAGGGGGCTGAAACAA+GGG + Chr4:2949340-2949359 None:intergenic 50.0%
GCAATAAGGTCCACTCCAGA+GGG + Chr4:2948719-2948738 None:intergenic 50.0%
GCTCATCAGTTTCGTGGTCT+TGG - Chr4:2948251-2948270 MsG0480018294.01.T01:CDS 50.0%
GGTTTCCGGAGAACCATTAC+CGG + Chr4:2950728-2950747 None:intergenic 50.0%
GTAGGAGGAAGGGTTACTCA+TGG - Chr4:2947828-2947847 MsG0480018294.01.T01:CDS 50.0%
TGCAATAAGGTCCACTCCAG+AGG + Chr4:2948720-2948739 None:intergenic 50.0%
TTTCAGCCCCCTCTATCTCA+AGG - Chr4:2949343-2949362 MsG0480018294.01.T01:intron 50.0%
! CCCCCAGACTAGGTCAATTT+TGG + Chr4:2949089-2949108 None:intergenic 50.0%
! TGCAGGTTGGAACGATTGAC+AGG - Chr4:2947873-2947892 MsG0480018294.01.T01:CDS 50.0%
GTGAGGCGTGAAAATGACGG+AGG - Chr4:2947794-2947813 MsG0480018294.01.T01:CDS 55.0%
GTGTAAGTTAGGGTCGGGGT+TGG - Chr4:2947769-2947788 MsG0480018294.01.T01:CDS 55.0%
TAAGTTAGGGTCGGGGTTGG+AGG - Chr4:2947772-2947791 MsG0480018294.01.T01:CDS 55.0%
! AGGCGTGAAAATGACGGAGG+AGG - Chr4:2947797-2947816 MsG0480018294.01.T01:CDS 55.0%
! TCACGCCGGTAATGGTTCTC+CGG - Chr4:2950720-2950739 MsG0480018294.01.T01:CDS 55.0%
!! TGTTGGTGTCACGCCGGTAA+TGG - Chr4:2950712-2950731 MsG0480018294.01.T01:CDS 55.0%
CTTCCTTGTGTGGCCCTGTG+TGG - Chr4:2948111-2948130 MsG0480018294.01.T01:intron 60.0%
TCTCCACACAGGGCCACACA+AGG + Chr4:2948117-2948136 None:intergenic 60.0%
GCTGCTCTCTCCCCCAGACT+AGG + Chr4:2949099-2949118 None:intergenic 65.0%
TAGGGTCGGGGTTGGAGGTG+AGG - Chr4:2947777-2947796 MsG0480018294.01.T01:CDS 65.0%
!! CGAGCGGGTCCGACTTGTTG+TGG + Chr4:2948364-2948383 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 2947767 2950877 2947767 ID=MsG0480018294.01;Name=MsG0480018294.01
Chr4 mRNA 2947767 2950877 2947767 ID=MsG0480018294.01.T01;Parent=MsG0480018294.01;Name=MsG0480018294.01.T01;_AED=0.33;_eAED=0.33;_QI=0|0|0|0.8|1|1|5|0|299
Chr4 exon 2950633 2950877 2950633 ID=MsG0480018294.01.T01:exon:1287;Parent=MsG0480018294.01.T01
Chr4 exon 2949770 2949843 2949770 ID=MsG0480018294.01.T01:exon:1286;Parent=MsG0480018294.01.T01
Chr4 exon 2948437 2948562 2948437 ID=MsG0480018294.01.T01:exon:1285;Parent=MsG0480018294.01.T01
Chr4 exon 2948154 2948353 2948154 ID=MsG0480018294.01.T01:exon:1284;Parent=MsG0480018294.01.T01
Chr4 exon 2947767 2948021 2947767 ID=MsG0480018294.01.T01:exon:1283;Parent=MsG0480018294.01.T01
Chr4 CDS 2950633 2950877 2950633 ID=MsG0480018294.01.T01:cds;Parent=MsG0480018294.01.T01
Chr4 CDS 2949770 2949843 2949770 ID=MsG0480018294.01.T01:cds;Parent=MsG0480018294.01.T01
Chr4 CDS 2948437 2948562 2948437 ID=MsG0480018294.01.T01:cds;Parent=MsG0480018294.01.T01
Chr4 CDS 2948154 2948353 2948154 ID=MsG0480018294.01.T01:cds;Parent=MsG0480018294.01.T01
Chr4 CDS 2947767 2948021 2947767 ID=MsG0480018294.01.T01:cds;Parent=MsG0480018294.01.T01
Gene Sequence

>MsG0480018294.01.T01

ATGTGTAAGTTAGGGTCGGGGTTGGAGGTGAGGCGTGAAAATGACGGAGGAGGTTATTTGCGTAGGAGGAAGGGTTACTCATGGAGTGTGTACGATTGTCTTGTGTTGCAGGTTGGAACGATTGACAGGTGGATGTGGAAACTCCATGTGTCGCAGTGTTACACAGTTAAATCAGCATATTCTTATCTAACGGCGATTGATACCAATCTCAATGACGGATTTGATAGCTTTTGTGGCTTAAAGCGGAGTAAAGGAAACCAACCCGAGTTGGAATTAGCAAAATGGGCTAGACTTTTGCAACATCCACATGATAAACCAAAAAGTGAAAACAATTCTGTTGCCGTTAACGGTGATGTTAAGGTTGTTGGGAACATGATTAAAAGTAACAGCTCAAATTCAATAGTGTCTCAACAACATCAACAACAAGAACAAGAACAACAACATGCTACCCCTAATTCTAAGAATAAACGTAGCCGTGAAAGTACTCAAAAGAGTGAAATGAAAATAAATCAACAAATGAATGGAGTGAAGAAAGGTAGAAGCAGTTCACAATGTATTGATCATATAATGGCAGAGAGAAAAAGGAGACAAGAATTGTCTGAGAAATTCATTGCACTTTCAGCTACTATTCCTGGCTTGAGCAAGACAGACAAGGCTTCAATACTTCAAGAAGCAATTGATTATGTGAAACAACTTAAAGAACGTGTTGATGAACTCGAGAAACAAGACAAAAATGTTGGTGTCACGCCGGTAATGGTTCTCCGGAAACCTTATTCATGTGGAAACAATAATTACAATGAAGCCACAAATTTAAGTGAAACAAATTCTGATGATGATTGTAACCATAATATCCTTCCAGAGATTGAAGCAAGAGTCACTGGAAAGGAAGTACTTTATTGA
Protein sequence

>MsG0480018294.01.T01

MCKLGSGLEVRRENDGGGYLRRRKGYSWSVYDCLVLQVGTIDRWMWKLHVSQCYTVKSAYSYLTAIDTNLNDGFDSFCGLKRSKGNQPELELAKWARLLQHPHDKPKSENNSVAVNGDVKVVGNMIKSNSSNSIVSQQHQQQEQEQQHATPNSKNKRSRESTQKSEMKINQQMNGVKKGRSSSQCIDHIMAERKRRQELSEKFIALSATIPGLSKTDKASILQEAIDYVKQLKERVDELEKQDKNVGVTPVMVLRKPYSCGNNNYNEATNLSETNSDDDCNHNILPEIEARVTGKEVLY*