Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018419.01.T01 | KEH15779.1 | 70 | 210 | 22 | 2 | 1 | 169 | 1 | 210 | 5.22E-95 | 287 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018419.01.T01 | A0A072TE13 | 70.000 | 210 | 22 | 2 | 1 | 169 | 1 | 210 | 2.49e-95 | 287 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0480018419.01.T01 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480018419.01.T01 | MTR_0558s0020 | 70.000 | 210 | 22 | 2 | 1 | 169 | 1 | 210 | 6.32e-99 | 287 |
| MsG0480018419.01.T01 | MTR_1g083640 | 64.444 | 225 | 24 | 2 | 1 | 169 | 1 | 225 | 3.85e-97 | 282 |
| MsG0480018419.01.T01 | MTR_2g014100 | 66.055 | 218 | 25 | 2 | 1 | 169 | 1 | 218 | 6.64e-97 | 280 |
| MsG0480018419.01.T01 | MTR_8g446020 | 77.647 | 170 | 37 | 1 | 1 | 169 | 1 | 170 | 2.04e-92 | 269 |
| MsG0480018419.01.T01 | MTR_0337s0040 | 77.193 | 171 | 37 | 2 | 1 | 169 | 1 | 171 | 9.36e-91 | 264 |
| MsG0480018419.01.T01 | MTR_3g008240 | 66.995 | 203 | 26 | 2 | 1 | 162 | 1 | 203 | 1.62e-89 | 260 |
| MsG0480018419.01.T01 | MTR_4g040430 | 65.842 | 202 | 25 | 2 | 1 | 162 | 1 | 198 | 1.79e-87 | 254 |
| MsG0480018419.01.T01 | MTR_6g090505 | 82.308 | 130 | 23 | 0 | 40 | 169 | 16 | 145 | 3.31e-77 | 234 |
| MsG0480018419.01.T01 | MTR_1g055240 | 87.395 | 119 | 15 | 0 | 51 | 169 | 3 | 121 | 1.13e-75 | 223 |
| MsG0480018419.01.T01 | MTR_0019s0290 | 77.444 | 133 | 30 | 0 | 37 | 169 | 4 | 136 | 1.77e-73 | 218 |
| MsG0480018419.01.T01 | MTR_5g071590 | 55.556 | 180 | 27 | 3 | 1 | 127 | 1 | 180 | 3.23e-60 | 185 |
| MsG0480018419.01.T01 | MTR_1281s0010 | 48.624 | 109 | 16 | 1 | 19 | 87 | 1 | 109 | 1.41e-27 | 100 |
| MsG0480018419.01.T01 | MTR_4g008820 | 61.111 | 72 | 17 | 3 | 1 | 62 | 125 | 195 | 7.01e-14 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTCATACTCAACAAGAAC+TGG | 0.395633 | 4:+4672952 | None:intergenic |
| CCATGATTCTTTATCACTAA+AGG | 0.406174 | 4:+4673191 | None:intergenic |
| TAAAGAATCATGGAAGTCTT+TGG | 0.408500 | 4:-4673181 | MsG0480018419.01.T01:intron |
| CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | 4:-4672775 | MsG0480018419.01.T01:CDS |
| AGCTGGAATATATTTGAAGT+TGG | 0.433136 | 4:-4672893 | MsG0480018419.01.T01:CDS |
| TGAATGTCTATAAGATATAT+AGG | 0.445219 | 4:-4672861 | MsG0480018419.01.T01:CDS |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | 4:-4672910 | MsG0480018419.01.T01:CDS |
| CGTTATGCTTGTAACAAAGA+AGG | 0.449439 | 4:-4673027 | MsG0480018419.01.T01:CDS |
| GCTTTAATAAATTCTAAAGC+TGG | 0.450264 | 4:-4672745 | MsG0480018419.01.T01:CDS |
| ATCTGAGAGTGGACAATTGA+CGG | 0.452529 | 4:-4673256 | MsG0480018419.01.T01:CDS |
| CTCAAGTAGCGCAAATGCTC+TGG | 0.460440 | 4:+4672805 | None:intergenic |
| GAAGTGATGGGTCAAGGAGT+TGG | 0.484292 | 4:-4672700 | MsG0480018419.01.T01:CDS |
| TTACAACCAACCCTTTCCTC+TGG | 0.485330 | 4:+4672916 | None:intergenic |
| GAGAACATCATATGAAGTGA+TGG | 0.507990 | 4:-4672713 | MsG0480018419.01.T01:CDS |
| TGTCTATAAGATATATAGGT+GGG | 0.523740 | 4:-4672857 | MsG0480018419.01.T01:CDS |
| TAACAAAGAAGGTTTCAAGA+AGG | 0.538422 | 4:-4673016 | MsG0480018419.01.T01:CDS |
| CCTTTAGTGATAAAGAATCA+TGG | 0.543522 | 4:-4673191 | MsG0480018419.01.T01:CDS |
| AACAAAGAAGGTTTCAAGAA+GGG | 0.545537 | 4:-4673015 | MsG0480018419.01.T01:CDS |
| AAATGCTCTGGTTTATGTAA+TGG | 0.552634 | 4:+4672817 | None:intergenic |
| TGAAAATTCAGTTATTGATG+CGG | 0.565546 | 4:-4673232 | MsG0480018419.01.T01:CDS |
| GTGATGGGTCAAGGAGTTGG+AGG | 0.568802 | 4:-4672697 | MsG0480018419.01.T01:CDS |
| TCATATGAAGTGATGGGTCA+AGG | 0.585703 | 4:-4672706 | MsG0480018419.01.T01:CDS |
| AGAACATCATATGAAGTGAT+GGG | 0.604657 | 4:-4672712 | MsG0480018419.01.T01:CDS |
| ATATCAACTTCATCTGAGAG+TGG | 0.614980 | 4:-4673267 | MsG0480018419.01.T01:CDS |
| AGAACTGGACTGTCACATGT+TGG | 0.620791 | 4:+4672967 | None:intergenic |
| GGGTCAAGGAGTTGGAGGCA+CGG | 0.621882 | 4:-4672692 | MsG0480018419.01.T01:CDS |
| ATGTCTATAAGATATATAGG+TGG | 0.643489 | 4:-4672858 | MsG0480018419.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAATTCACAAAATAAGAAAT+CGG | - | Chr4:4672878-4672897 | MsG0480018419.01.T01:CDS | 20.0% |
| !! | TGAATGTCTATAAGATATAT+AGG | - | Chr4:4673084-4673103 | MsG0480018419.01.T01:intron | 20.0% |
| !! | TTTAATTATGCATATAACAC+TGG | - | Chr4:4672822-4672841 | MsG0480018419.01.T01:CDS | 20.0% |
| ! | ACAAAATAAGAAATCGGATT+AGG | - | Chr4:4672884-4672903 | MsG0480018419.01.T01:CDS | 25.0% |
| ! | ATGTCTATAAGATATATAGG+TGG | - | Chr4:4673087-4673106 | MsG0480018419.01.T01:intron | 25.0% |
| ! | TGAAAATTCAGTTATTGATG+CGG | - | Chr4:4672713-4672732 | MsG0480018419.01.T01:CDS | 25.0% |
| ! | TGTCTATAAGATATATAGGT+GGG | - | Chr4:4673088-4673107 | MsG0480018419.01.T01:intron | 25.0% |
| !!! | CTTATTTTGTGAATTGTTCT+TGG | + | Chr4:4672874-4672893 | None:intergenic | 25.0% |
| !!! | GCTTTAATAAATTCTAAAGC+TGG | - | Chr4:4673200-4673219 | MsG0480018419.01.T01:CDS | 25.0% |
| AACAAAGAAGGTTTCAAGAA+GGG | - | Chr4:4672930-4672949 | MsG0480018419.01.T01:CDS | 30.0% | |
| AGAACATCATATGAAGTGAT+GGG | - | Chr4:4673233-4673252 | MsG0480018419.01.T01:CDS | 30.0% | |
| CCATGATTCTTTATCACTAA+AGG | + | Chr4:4672757-4672776 | None:intergenic | 30.0% | |
| CCTTTAGTGATAAAGAATCA+TGG | - | Chr4:4672754-4672773 | MsG0480018419.01.T01:CDS | 30.0% | |
| TAAAGAAAAACCAGAGGAAA+GGG | - | Chr4:4673019-4673038 | MsG0480018419.01.T01:CDS | 30.0% | |
| TAAAGAATCATGGAAGTCTT+TGG | - | Chr4:4672764-4672783 | MsG0480018419.01.T01:CDS | 30.0% | |
| TAACAAAGAAGGTTTCAAGA+AGG | - | Chr4:4672929-4672948 | MsG0480018419.01.T01:CDS | 30.0% | |
| ! | AAATGCTCTGGTTTATGTAA+TGG | + | Chr4:4673131-4673150 | None:intergenic | 30.0% |
| ! | AGCTGGAATATATTTGAAGT+TGG | - | Chr4:4673052-4673071 | MsG0480018419.01.T01:intron | 30.0% |
| ATAACACTGGATTCTCTGTT+AGG | - | Chr4:4672835-4672854 | MsG0480018419.01.T01:CDS | 35.0% | |
| ATATCAACTTCATCTGAGAG+TGG | - | Chr4:4672678-4672697 | MsG0480018419.01.T01:CDS | 35.0% | |
| CGAGAAGTAAATGAAGTTCA+AGG | - | Chr4:4673170-4673189 | MsG0480018419.01.T01:intron | 35.0% | |
| CGTTATGCTTGTAACAAAGA+AGG | - | Chr4:4672918-4672937 | MsG0480018419.01.T01:CDS | 35.0% | |
| CTAAAGAAAAACCAGAGGAA+AGG | - | Chr4:4673018-4673037 | MsG0480018419.01.T01:CDS | 35.0% | |
| GAAAGCTAAAGAAAAACCAG+AGG | - | Chr4:4673013-4673032 | MsG0480018419.01.T01:CDS | 35.0% | |
| GAGAACATCATATGAAGTGA+TGG | - | Chr4:4673232-4673251 | MsG0480018419.01.T01:CDS | 35.0% | |
| GTCTGCTTAATCCTAAAAAC+AGG | - | Chr4:4672955-4672974 | MsG0480018419.01.T01:CDS | 35.0% | |
| TTCTCATACTCAACAAGAAC+TGG | + | Chr4:4672996-4673015 | None:intergenic | 35.0% | |
| ATCTGAGAGTGGACAATTGA+CGG | - | Chr4:4672689-4672708 | MsG0480018419.01.T01:CDS | 40.0% | |
| !! | TCACATGTTGGCCTGTTTTT+AGG | + | Chr4:4672969-4672988 | None:intergenic | 40.0% |
| !! | TCATATGAAGTGATGGGTCA+AGG | - | Chr4:4673239-4673258 | MsG0480018419.01.T01:CDS | 40.0% |
| AGAACTGGACTGTCACATGT+TGG | + | Chr4:4672981-4673000 | None:intergenic | 45.0% | |
| GAAAAACCAGAGGAAAGGGT+TGG | - | Chr4:4673023-4673042 | MsG0480018419.01.T01:CDS | 45.0% | |
| TTACAACCAACCCTTTCCTC+TGG | + | Chr4:4673032-4673051 | None:intergenic | 45.0% | |
| !! | GAAAGGGTTGGTTGTAAAGC+TGG | - | Chr4:4673035-4673054 | MsG0480018419.01.T01:CDS | 45.0% |
| CTCAAGTAGCGCAAATGCTC+TGG | + | Chr4:4673143-4673162 | None:intergenic | 50.0% | |
| !! | GAAGTGATGGGTCAAGGAGT+TGG | - | Chr4:4673245-4673264 | MsG0480018419.01.T01:CDS | 50.0% |
| !! | GTGATGGGTCAAGGAGTTGG+AGG | - | Chr4:4673248-4673267 | MsG0480018419.01.T01:CDS | 55.0% |
| GGGTCAAGGAGTTGGAGGCA+CGG | - | Chr4:4673253-4673272 | MsG0480018419.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 4672669 | 4673298 | 4672669 | ID=MsG0480018419.01;Name=MsG0480018419.01 |
| Chr4 | mRNA | 4672669 | 4673298 | 4672669 | ID=MsG0480018419.01.T01;Parent=MsG0480018419.01;Name=MsG0480018419.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|1|1|2|0|169 |
| Chr4 | exon | 4673182 | 4673298 | 4673182 | ID=MsG0480018419.01.T01:exon:1720;Parent=MsG0480018419.01.T01 |
| Chr4 | exon | 4672669 | 4673061 | 4672669 | ID=MsG0480018419.01.T01:exon:1719;Parent=MsG0480018419.01.T01 |
| Chr4 | CDS | 4673182 | 4673298 | 4673182 | ID=MsG0480018419.01.T01:cds;Parent=MsG0480018419.01.T01 |
| Chr4 | CDS | 4672669 | 4673061 | 4672669 | ID=MsG0480018419.01.T01:cds;Parent=MsG0480018419.01.T01 |
| Gene Sequence |
| Protein sequence |
