AlfalfaGEDB | MsG0480018597.01

M. sativa cultivar ZhongmuNo.1 / MsG0480018597.01

KEGG: -
GO: GO:0003677
PFAM: PF00249

Nr:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018597.01.T03	XP_004506787.1	81.25	48	9	1	48	58	105	4.94E-17	82.4
MsG0480018597.01.T02	QSD99762.1	90.789	76	7	1	76	23	98	4.78E-42	147
MsG0480018597.01.T01	QSD99762.1	90	80	8	1	80	19	98	1.48E-44	153

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018597.01.T01	Q8S9H7	54.118	85	39	3	87	15	99	6.05E-30	110
MsG0480018597.01.T02	Q8S9H7	54.217	83	38	1	83	17	99	7.01E-29	107
MsG0480018597.01.T03	Q8S9H7	54.167	48	22	3	50	52	99	3.19E-12	61.6

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018597.01.T01	A0A896WD31	90.000	80	8	1	80	19	98	7.07e-45	153
MsG0480018597.01.T02	A0A896WD31	90.789	76	7	1	76	23	98	2.28e-42	147
MsG0480018597.01.T03	A0A1S2YLX7	81.250	48	9	1	48	58	105	2.36e-17	82.4

TFs/TRs:

Gene ID	Type	Classification
MsG0480018597.01.T01	TF	MYB-related
MsG0480018597.01.T02	TF	MYB-related

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018597.01.T03	MTR_7g067080	69.565	46	13	1	3	48	61	105	3.20e-14	64.3
MsG0480018597.01.T02	MTR_7g067080	71.605	81	21	2	1	81	27	105	8.89e-34	117
MsG0480018597.01.T02	MTR_3g462790	62.821	78	28	1	5	81	38	115	7.06e-29	105
MsG0480018597.01.T02	MTR_5g069710	62.500	80	29	1	3	81	22	101	1.55e-28	103
MsG0480018597.01.T02	MTR_5g081860	61.333	75	28	1	8	82	25	98	3.04e-26	97.4
MsG0480018597.01.T02	MTR_3g111880	46.667	75	40	0	8	82	5	79	3.72e-21	79.7
MsG0480018597.01.T02	MTR_8g077360	51.429	70	33	1	8	77	3	71	6.11e-21	78.6
MsG0480018597.01.T02	MTR_8g077380	51.429	70	33	1	8	77	3	71	6.57e-21	78.6
MsG0480018597.01.T02	MTR_8g077390	51.429	70	33	1	8	77	3	71	6.57e-21	78.6
MsG0480018597.01.T02	MTR_6g004250	47.887	71	37	0	4	74	4	74	1.70e-20	77.8
MsG0480018597.01.T02	MTR_1g022290	45.070	71	39	0	8	78	5	75	2.21e-20	77.8
MsG0480018597.01.T02	MTR_3g116720	50.000	66	33	0	10	75	16	81	2.20e-19	75.1
MsG0480018597.01.T02	MTR_7g089210	53.846	65	30	0	12	76	14	78	6.32e-19	73.9
MsG0480018597.01.T02	MTR_5g020170	48.438	64	33	0	9	72	18	81	1.54e-18	73.2
MsG0480018597.01.T02	MTR_3g013440	45.205	73	39	1	10	81	10	82	1.22e-17	70.9
MsG0480018597.01.T02	MTR_8g077420	44.118	68	37	1	10	77	5	71	2.85e-17	69.3
MsG0480018597.01.T02	MTR_5g020540	45.312	64	34	1	7	70	1	63	7.51e-17	68.2
MsG0480018597.01.T02	MTR_3g111920	39.189	74	45	0	10	83	3	76	2.20e-15	64.7
MsG0480018597.01.T02	MTR_1g033600	37.681	69	43	0	8	76	10	78	4.82e-15	65.5
MsG0480018597.01.T02	MTR_8g063870	41.667	72	42	0	8	79	6	77	4.49e-14	65.1
MsG0480018597.01.T02	MTR_1g086180	48.387	62	31	1	5	65	6	67	1.01e-12	58.5
MsG0480018597.01.T02	MTR_8g063600	38.235	68	42	0	9	76	9	76	1.02e-12	61.6
MsG0480018597.01.T02	MTR_1g111830	36.111	72	46	0	8	79	22	93	1.28e-12	60.8
MsG0480018597.01.T02	MTR_7g110580	45.902	61	32	1	8	67	6	66	3.56e-11	54.3
MsG0480018597.01.T01	MTR_7g067080	71.765	85	22	2	1	85	23	105	4.73e-36	124
MsG0480018597.01.T01	MTR_5g069710	61.446	83	31	1	4	85	19	101	2.26e-29	106
MsG0480018597.01.T01	MTR_3g462790	56.667	90	34	2	1	85	26	115	2.88e-29	106
MsG0480018597.01.T01	MTR_5g081860	58.140	86	34	2	1	86	15	98	1.51e-27	100
MsG0480018597.01.T01	MTR_3g111880	46.667	75	40	0	12	86	5	79	7.66e-21	79.0
MsG0480018597.01.T01	MTR_8g077360	51.429	70	33	1	12	81	3	71	1.22e-20	78.2
MsG0480018597.01.T01	MTR_8g077380	51.429	70	33	1	12	81	3	71	1.35e-20	77.8
MsG0480018597.01.T01	MTR_8g077390	51.429	70	33	1	12	81	3	71	1.35e-20	77.8
MsG0480018597.01.T01	MTR_1g022290	45.070	71	39	0	12	82	5	75	3.53e-20	77.4
MsG0480018597.01.T01	MTR_6g004250	47.887	71	37	0	8	78	4	74	3.65e-20	77.0
MsG0480018597.01.T01	MTR_3g116720	50.000	66	33	0	14	79	16	81	3.96e-19	74.3
MsG0480018597.01.T01	MTR_7g089210	53.846	65	30	0	16	80	14	78	8.96e-19	73.9
MsG0480018597.01.T01	MTR_5g020170	48.438	64	33	0	13	76	18	81	2.83e-18	72.8
MsG0480018597.01.T01	MTR_3g013440	45.205	73	39	1	14	85	10	82	2.40e-17	70.1
MsG0480018597.01.T01	MTR_8g077420	44.118	68	37	1	14	81	5	71	5.23e-17	68.9
MsG0480018597.01.T01	MTR_5g020540	45.312	64	34	1	11	74	1	63	1.24e-16	67.8
MsG0480018597.01.T01	MTR_3g111920	39.189	74	45	0	14	87	3	76	3.53e-15	64.7
MsG0480018597.01.T01	MTR_1g033600	37.681	69	43	0	12	80	10	78	1.32e-14	64.7
MsG0480018597.01.T01	MTR_8g063870	41.667	72	42	0	12	83	6	77	9.31e-14	64.3
MsG0480018597.01.T01	MTR_1g111830	36.111	72	46	0	12	83	22	93	1.30e-12	61.2
MsG0480018597.01.T01	MTR_8g063600	38.235	68	42	0	13	80	9	76	1.49e-12	61.2
MsG0480018597.01.T01	MTR_1g086180	48.387	62	31	1	9	69	6	67	3.09e-12	57.4
MsG0480018597.01.T01	MTR_7g110580	45.902	61	32	1	12	71	6	66	8.38e-11	53.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018597.01.T03	AT5G58900	56.522	46	20	0	3	48	60	105	2.95e-12	58.9
MsG0480018597.01.T03	AT2G38090	58.333	48	19	1	3	50	55	101	3.86e-12	58.5
MsG0480018597.01.T02	AT2G38090	66.265	83	26	2	1	83	21	101	7.58e-32	112
MsG0480018597.01.T02	AT5G58900	61.111	72	28	0	10	81	34	105	2.98e-26	97.8
MsG0480018597.01.T02	AT3G11280	58.824	68	28	0	10	77	33	100	1.81e-22	87.4
MsG0480018597.01.T02	AT3G11280	58.824	68	28	0	10	77	33	100	1.81e-22	87.4
MsG0480018597.01.T02	AT5G05790	57.971	69	29	0	8	76	29	97	2.10e-22	87.4
MsG0480018597.01.T02	AT5G05790	57.971	69	29	0	8	76	29	97	2.10e-22	87.4
MsG0480018597.01.T02	AT4G39250	45.714	70	38	0	10	79	14	83	1.02e-20	79.0
MsG0480018597.01.T02	AT1G75250	50.000	68	34	0	7	74	9	76	2.18e-20	78.2
MsG0480018597.01.T02	AT1G75250	49.296	71	36	0	7	77	9	79	5.82e-20	77.8
MsG0480018597.01.T02	AT5G01200	52.564	78	35	2	1	77	22	98	1.76e-19	79.7
MsG0480018597.01.T02	AT2G21650	45.588	68	37	0	10	77	14	81	5.50e-18	72.0
MsG0480018597.01.T02	AT1G19510	45.070	71	39	0	13	83	15	85	4.49e-17	69.7
MsG0480018597.01.T02	AT2G18328	46.032	63	34	0	14	76	15	77	2.54e-14	62.0
MsG0480018597.01.T02	AT4G36570	58.140	43	18	0	10	52	12	54	6.00e-14	60.5
MsG0480018597.01.T02	AT1G49010	42.029	69	37	1	7	72	5	73	8.13e-13	62.0
MsG0480018597.01.T01	AT2G38090	66.667	87	27	2	1	87	17	101	7.55e-34	117
MsG0480018597.01.T01	AT5G58900	52.747	91	36	1	2	85	15	105	3.61e-26	97.8
MsG0480018597.01.T01	AT5G05790	57.971	69	29	0	12	80	29	97	2.78e-22	87.4
MsG0480018597.01.T01	AT5G05790	57.971	69	29	0	12	80	29	97	2.78e-22	87.4
MsG0480018597.01.T01	AT3G11280	58.824	68	28	0	14	81	33	100	3.41e-22	87.0
MsG0480018597.01.T01	AT3G11280	58.824	68	28	0	14	81	33	100	3.41e-22	87.0
MsG0480018597.01.T01	AT5G01200	52.439	82	37	2	1	81	18	98	2.66e-21	84.7
MsG0480018597.01.T01	AT4G39250	45.714	70	38	0	14	83	14	83	2.36e-20	78.2
MsG0480018597.01.T01	AT1G75250	50.000	68	34	0	11	78	9	76	3.69e-20	77.4
MsG0480018597.01.T01	AT1G75250	49.296	71	36	0	11	81	9	79	1.05e-19	77.4
MsG0480018597.01.T01	AT2G21650	45.588	68	37	0	14	81	14	81	1.01e-17	71.6
MsG0480018597.01.T01	AT1G19510	45.070	71	39	0	17	87	15	85	6.63e-17	69.3
MsG0480018597.01.T01	AT2G18328	46.032	63	34	0	18	80	15	77	3.90e-14	61.6
MsG0480018597.01.T01	AT4G36570	58.140	43	18	0	14	56	12	54	1.16e-13	60.1
MsG0480018597.01.T01	AT1G49010	42.029	69	37	1	11	76	5	73	1.73e-12	61.2

Find 16 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGTTAGTGTTATTGAATC+AGG	0.205600	4:+7172887	MsG0480018597.01.T01:CDS
CGCTTTGGCTTTATATGATA+AGG	0.327997	4:+7172775	MsG0480018597.01.T01:CDS
GTAGTAGTATACATAGGAAA+AGG	0.418459	4:-7172917	None:intergenic
CAAGTTGTTTGAGAACGCTT+TGG	0.423370	4:+7172760	MsG0480018597.01.T01:CDS
ACTCTCTTGGAACCACCAAT+TGG	0.440479	4:-7172705	None:intergenic
GCTACCATCATCCAACGGTC+GGG	0.445026	4:-7172803	None:intergenic
ACATCACTAATAGTCTTACT+TGG	0.467585	4:-7172836	None:intergenic
CTACCATCATCCAACGGTCG+GGG	0.477795	4:-7172802	None:intergenic
TGCTACCATCATCCAACGGT+CGG	0.501556	4:-7172804	None:intergenic
AAGTTAGTGTTATTGAATCA+GGG	0.513183	4:+7172888	MsG0480018597.01.T01:CDS
AGAGTAAAAGAATTACCAAA+TGG	0.523493	4:+7172723	MsG0480018597.01.T01:CDS
CATTTCCAAAGTAAAAGAAC+TGG	0.532535	4:-7172939	None:intergenic
TAGTAGTATACATAGGAAAA+GGG	0.549620	4:-7172916	None:intergenic
GAACTGGTAGTAGTATACAT+AGG	0.593175	4:-7172923	None:intergenic
TACCATCATCCAACGGTCGG+GGG	0.662362	4:-7172801	None:intergenic
TTTCTGCTACCATCATCCAA+CGG	0.713612	4:-7172808	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGAGTAAAAGAATTACCAAA+TGG	+	Chr4:7172723-7172742	MsG0480018597.01.T01:CDS	25.0%
!	TAGTAGTATACATAGGAAAA+GGG	-	Chr4:7172919-7172938	None:intergenic	25.0%
!!	AAGTTAGTGTTATTGAATCA+GGG	+	Chr4:7172888-7172907	MsG0480018597.01.T01:CDS	25.0%
!!!	AGTTCTTTTACTTTGGAAAT+GGG	+	Chr4:7172941-7172960	MsG0480018597.01.T01:CDS	25.0%
	CATTTCCAAAGTAAAAGAAC+TGG	-	Chr4:7172942-7172961	None:intergenic	30.0%
	GTAGTAGTATACATAGGAAA+AGG	-	Chr4:7172920-7172939	None:intergenic	30.0%
!	ACATCACTAATAGTCTTACT+TGG	-	Chr4:7172839-7172858	None:intergenic	30.0%
!	GAAGTTAGTGTTATTGAATC+AGG	+	Chr4:7172887-7172906	MsG0480018597.01.T01:CDS	30.0%
!	TACTACCAGTTCTTTTACTT+TGG	+	Chr4:7172934-7172953	MsG0480018597.01.T01:CDS	30.0%
!	TGGTAATTCTTTTACTCTCT+TGG	-	Chr4:7172721-7172740	None:intergenic	30.0%
!!	CAGTTCTTTTACTTTGGAAA+TGG	+	Chr4:7172940-7172959	MsG0480018597.01.T01:CDS	30.0%
!!!	GTTTTCTTCTAAAGTCCATT+TGG	-	Chr4:7172741-7172760	None:intergenic	30.0%
	GAACTGGTAGTAGTATACAT+AGG	-	Chr4:7172926-7172945	None:intergenic	35.0%
!!	CGCTTTGGCTTTATATGATA+AGG	+	Chr4:7172775-7172794	MsG0480018597.01.T01:CDS	35.0%
	CAAGTTGTTTGAGAACGCTT+TGG	+	Chr4:7172760-7172779	MsG0480018597.01.T01:CDS	40.0%
	TTTCTGCTACCATCATCCAA+CGG	-	Chr4:7172811-7172830	None:intergenic	40.0%
	ACTCTCTTGGAACCACCAAT+TGG	-	Chr4:7172708-7172727	None:intergenic	45.0%
	TGCTACCATCATCCAACGGT+CGG	-	Chr4:7172807-7172826	None:intergenic	50.0%
	CTACCATCATCCAACGGTCG+GGG	-	Chr4:7172805-7172824	None:intergenic	55.0%
	GCTACCATCATCCAACGGTC+GGG	-	Chr4:7172806-7172825	None:intergenic	55.0%
	TACCATCATCCAACGGTCGG+GGG	-	Chr4:7172804-7172823	None:intergenic	55.0%
	ATAAGGACACCCCCGACCGT+TGG	+	Chr4:7172792-7172811	MsG0480018597.01.T01:CDS	60.0%
	CACCCCCGACCGTTGGATGA+TGG	+	Chr4:7172799-7172818	MsG0480018597.01.T01:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	7172704	7172967	7172704	ID=MsG0480018597.01;Name=MsG0480018597.01
Chr4	mRNA	7172704	7172967	7172704	ID=MsG0480018597.01.T01;Parent=MsG0480018597.01;Name=MsG0480018597.01.T01;_AED=0.48;_eAED=0.48;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|87
Chr4	exon	7172704	7172967	7172704	ID=MsG0480018597.01.T01:exon:1794;Parent=MsG0480018597.01.T01
Chr4	CDS	7172704	7172967	7172704	ID=MsG0480018597.01.T01:cds;Parent=MsG0480018597.01.T01
Chr4	mRNA	7172716	7172967	7172716	ID=MsG0480018597.01.T02;Parent=MsG0480018597.01;Name=MsG0480018597.01.T02;_AED=0.48;_eAED=0.48;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|83
Chr4	exon	7172716	7172967	7172716	ID=MsG0480018597.01.T02:exon:1795;Parent=MsG0480018597.01.T02
Chr4	CDS	7172716	7172967	7172716	ID=MsG0480018597.01.T02:cds;Parent=MsG0480018597.01.T02
Chr4	mRNA	7172815	7172967	7172815	ID=MsG0480018597.01.T03;Parent=MsG0480018597.01;Name=MsG0480018597.01.T03;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|50
Chr4	exon	7172815	7172967	7172815	ID=MsG0480018597.01.T03:exon:1796;Parent=MsG0480018597.01.T03
Chr4	CDS	7172815	7172967	7172815	ID=MsG0480018597.01.T03:cds;Parent=MsG0480018597.01.T03

Gene Sequence

>MsG0480018597.01.T03

ATGATGGTAGCAGAAATGATTCCAAGTAAGACTATTAGTGATGTGATGAAACATTATAGAGATCATGAAGAAGAAGTTAGTGTTATTGAATCAGGGTTGATCCCTTTTCCTATGTATACTACTACCAGTTCTTTTACTTTGGAAATGGGTTAA

>MsG0480018597.01.T02

TTCCAAGAGAGTAAAAGAATTACCAAATGGACTTTAGAAGAAAACAAGTTGTTTGAGAACGCTTTGGCTTTATATGATAAGGACACCCCCGACCGTTGGATGATGGTAGCAGAAATGATTCCAAGTAAGACTATTAGTGATGTGATGAAACATTATAGAGATCATGAAGAAGAAGTTAGTGTTATTGAATCAGGGTTGATCCCTTTTCCTATGTATACTACTACCAGTTCTTTTACTTTGGAAATGGGTTAA

>MsG0480018597.01.T01

TCCAATTGGTGGTTCCAAGAGAGTAAAAGAATTACCAAATGGACTTTAGAAGAAAACAAGTTGTTTGAGAACGCTTTGGCTTTATATGATAAGGACACCCCCGACCGTTGGATGATGGTAGCAGAAATGATTCCAAGTAAGACTATTAGTGATGTGATGAAACATTATAGAGATCATGAAGAAGAAGTTAGTGTTATTGAATCAGGGTTGATCCCTTTTCCTATGTATACTACTACCAGTTCTTTTACTTTGGAAATGGGTTAA

Protein sequence

>MsG0480018597.01.T03

MMVAEMIPSKTISDVMKHYRDHEEEVSVIESGLIPFPMYTTTSSFTLEMG*

>MsG0480018597.01.T02

FQESKRITKWTLEENKLFENALALYDKDTPDRWMMVAEMIPSKTISDVMKHYRDHEEEVSVIESGLIPFPMYTTTSSFTLEMG*

>MsG0480018597.01.T01

SNWWFQESKRITKWTLEENKLFENALALYDKDTPDRWMMVAEMIPSKTISDVMKHYRDHEEEVSVIESGLIPFPMYTTTSSFTLEMG*