AlfalfaGEDB | MsG0480019011.01

M. sativa cultivar ZhongmuNo.1 / MsG0480019011.01

KEGG: -
GO: GO:0000160 GO:0003677
PFAM: PF00072 PF00249

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019011.01.T01	XP_013455021.2	87.859	313	36	1	1	313	1	311	0	577

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019011.01.T01	Q5N6V8	38.824	255	127	4	6	231	2	256	1.86E-53	187

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019011.01.T01	A0A396I0H3	85.350	314	43	2	1	313	1	312	0.0	551

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TFs/TRs:

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Gene ID	Type	Classification
MsG0480019011.01.T01	TF	GARP-ARR-B

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019011.01.T01	MTR_4g021760	84.713	314	45	2	1	313	1	312	0.0	546
MsG0480019011.01.T01	MTR_4g021855	66.776	304	89	5	1	295	1	301	2.51e-139	396
MsG0480019011.01.T01	MTR_4g021845	85.845	219	29	1	1	219	1	217	8.04e-138	389
MsG0480019011.01.T01	MTR_4g021790	76.033	242	56	1	1	242	1	240	1.79e-129	370
MsG0480019011.01.T01	MTR_5g055260	60.173	231	63	5	1	231	1	202	2.59e-86	259
MsG0480019011.01.T01	MTR_4g023980	78.417	139	28	1	55	193	35	171	9.43e-74	225
MsG0480019011.01.T01	MTR_6g045327	45.752	306	143	9	1	296	1	293	3.36e-71	225
MsG0480019011.01.T01	MTR_1g032570	37.808	365	160	9	10	309	11	373	1.64e-68	225
MsG0480019011.01.T01	MTR_3g086100	39.921	253	119	5	10	231	13	263	9.16e-57	192
MsG0480019011.01.T01	MTR_8g079940	39.695	262	125	6	9	239	24	283	5.31e-56	190

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019011.01.T01	AT2G01760	38.403	263	121	5	7	232	4	262	1.51e-55	185
MsG0480019011.01.T01	AT1G67710	40.075	267	124	8	11	243	8	272	2.32e-53	182
MsG0480019011.01.T01	AT3G16857	36.039	308	139	9	10	259	33	340	1.32e-50	178
MsG0480019011.01.T01	AT3G16857	36.688	308	137	11	10	259	33	340	1.35e-50	177
MsG0480019011.01.T01	AT2G01760	35.052	291	120	7	7	232	4	290	1.35e-49	170
MsG0480019011.01.T01	AT4G31920	36.481	233	138	2	10	232	13	245	3.97e-46	164
MsG0480019011.01.T01	AT2G25180	38.750	240	123	6	10	226	13	251	1.07e-43	158
MsG0480019011.01.T01	AT3G16857	34.944	269	117	9	49	259	2	270	2.12e-39	146
MsG0480019011.01.T01	AT5G58080	37.008	254	126	7	10	236	14	260	3.90e-39	145
MsG0480019011.01.T01	AT1G67710	40.191	209	90	7	68	243	1	207	7.11e-36	134

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Find 58 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAACACTTGCAAAGA+AGG	0.435703	4:-13261384	MsG0480019011.01.T01:CDS
AAAAGTGTTGCTCATAATGT+TGG	0.490442	4:+13260581	None:intergenic
AAAATGAACCTGTAAATGGC+TGG	0.527802	4:+13261139	None:intergenic
AAGTCATAAACATCCATGTC+TGG	0.491105	4:+13261781	None:intergenic
AATAAAGAATATGAAATTGT+TGG	0.416766	4:-13261461	MsG0480019011.01.T01:CDS
AATAAATTCTTACTAATGAC+AGG	0.461511	4:+13261727	None:intergenic
AATGTGGGGCTTGCGAATAT+TGG	0.385194	4:-13261559	MsG0480019011.01.T01:CDS
AATTGCAATAACCTTGAGAC+CGG	0.581075	4:+13262055	None:intergenic
ACATATTGCCAGCCATTTAC+AGG	0.282271	4:-13261147	MsG0480019011.01.T01:intron
ACATCTCCTCAATAGAGTTA+AGG	0.467190	4:+13262015	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAGAAACACTTGCAAAGA+AGG	-	Chr4:13261226-13261245	MsG0480019011.01.T01:intron	30.0%
AACTGAGAAACTATAGACTT+AGG	+	Chr4:13261539-13261558	None:intergenic	30.0%
AGGAAAGAATCATACTATCA+AGG	+	Chr4:13261313-13261332	None:intergenic	30.0%
AGTAGCATATTATCAACAAG+AGG	+	Chr4:13261732-13261751	None:intergenic	30.0%
ATATGAAATTGTTGGAAGCT+CGG	-	Chr4:13261157-13261176	MsG0480019011.01.T01:CDS	30.0%
ATGATTTATCATCACTTGTG+TGG	-	Chr4:13262086-13262105	MsG0480019011.01.T01:CDS	30.0%
CATGAAATCTATTGAATGTG+GGG	-	Chr4:13261037-13261056	MsG0480019011.01.T01:intron	30.0%
CATGTTGCAAGAATTAGAAA+CGG	-	Chr4:13261116-13261135	MsG0480019011.01.T01:intron	30.0%
GGTTCAAGAAAATAGAAAAC+GGG	-	Chr4:13261181-13261200	MsG0480019011.01.T01:CDS	30.0%
GTTCAAGAAAATAGAAAACG+GGG	-	Chr4:13261182-13261201	MsG0480019011.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	13260521	13262111	13260521	ID=MsG0480019011.01;Name=MsG0480019011.01
Chr4	mRNA	13260521	13262111	13260521	ID=MsG0480019011.01.T01;Parent=MsG0480019011.01;Name=MsG0480019011.01.T01;_AED=0.08;_eAED=0.08;_QI=0\|0\|0\|1\|1\|1\|5\|0\|313
Chr4	exon	13261994	13262111	13261994	ID=MsG0480019011.01.T01:exon:8307;Parent=MsG0480019011.01.T01
Chr4	exon	13261740	13261889	13261740	ID=MsG0480019011.01.T01:exon:8306;Parent=MsG0480019011.01.T01
Chr4	exon	13261315	13261626	13261315	ID=MsG0480019011.01.T01:exon:8305;Parent=MsG0480019011.01.T01
Chr4	exon	13261148	13261224	13261148	ID=MsG0480019011.01.T01:exon:8304;Parent=MsG0480019011.01.T01
Chr4	exon	13260521	13260805	13260521	ID=MsG0480019011.01.T01:exon:8303;Parent=MsG0480019011.01.T01
Chr4	CDS	13261994	13262111	13261994	ID=MsG0480019011.01.T01:cds;Parent=MsG0480019011.01.T01
Chr4	CDS	13261740	13261889	13261740	ID=MsG0480019011.01.T01:cds;Parent=MsG0480019011.01.T01
Chr4	CDS	13261315	13261626	13261315	ID=MsG0480019011.01.T01:cds;Parent=MsG0480019011.01.T01
Chr4	CDS	13261148	13261224	13261148	ID=MsG0480019011.01.T01:cds;Parent=MsG0480019011.01.T01
Chr4	CDS	13260521	13260805	13260521	ID=MsG0480019011.01.T01:cds;Parent=MsG0480019011.01.T01

Gene Sequence

>MsG0480019011.01.T01

ATGGCAGTTTTCACTGACAACCCATCATTCCCAGCCGGTCTCAAGGTTATTGCAATTGATCATGACATCACTCTCCTTAACTCTATTGAGGAGATGTGTAATCGATATCATTATCAAGTTACCAAATGTAGTATGGCTTCTGATGCCTTCAACATGTTGGAAAGAAAGGACTGTTTCGATGTAATGTTGATTGATGCTCATATGCCAGACATGGATGTTTATGACTTTGTCCAAAATGTTACCCTACAACTCAACATTCCTGTCATTATGATGGCTGTTGATTCCACAAAAAGTTCTATCATGAAATCTATTGAATGTGGGGCTTGCGAATATTGGACTAAGCCTTTGGTTGAAAAAGAGGTCAAGATCATGTGGCAACATGTTGCAAGAATTAGAAACGGTTTGATTGAAAATAAAGAATATGAAATTGTTGGAAGCTCGGTGGTTCAAGAAAATAGAAAACGGGGTAGAGAAGATGCTAATGCCTCAAAAGAAACACTTGCAAAGAAGGCCCGTTTGTCTTGGTCACGAGAATTGCATCAGCAGTTTCTACGTGCAGTCAATCAACTAGGCCTTGATAGGGCAACGCCAAAAAAGATTCTGAAAATCATGGATGTTCCCGATATGAAAAAAGAACATATTGCCAGCCATTTACAGAAATATAGAAATCGGTTAAAGAGCTCAACAGAAGAGACAAAAGGTAGGATGAAAAAATCAAAGACGAGTCCTCTTGAAACAAAATTTGACTTAGAAGCTACTCACTCAATGCCGGAGCAGGACCAGAGTTTTCTCCTGAATTCTGCGACACAATGTCGCGACAATTGCGATGCACAACGACATTGGGTGGAAATCGGTGAATCCAACATTATGAGCAACACTTTTTCAGACTTGCCCAACTTGTTCCCATATGTTGATGATGATTTATCATCACTTGTGTGGTGA

Protein sequence

>MsG0480019011.01.T01

MAVFTDNPSFPAGLKVIAIDHDITLLNSIEEMCNRYHYQVTKCSMASDAFNMLERKDCFDVMLIDAHMPDMDVYDFVQNVTLQLNIPVIMMAVDSTKSSIMKSIECGACEYWTKPLVEKEVKIMWQHVARIRNGLIENKEYEIVGSSVVQENRKRGREDANASKETLAKKARLSWSRELHQQFLRAVNQLGLDRATPKKILKIMDVPDMKKEHIASHLQKYRNRLKSSTEETKGRMKKSKTSPLETKFDLEATHSMPEQDQSFLLNSATQCRDNCDAQRHWVEIGESNIMSNTFSDLPNLFPYVDDDLSSLVW*