AlfalfaGEDB | MsG0480019268.01

M. sativa cultivar ZhongmuNo.1 / MsG0480019268.01

KEGG: -
GO: GO:0000982 GO:0000987 GO:0003677 GO:0045944 GO:0046983
PFAM: PF00319

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019268.01.T01	XP_003605334.1	95.597	159	7	0	1	159	1	159	1.12E-99	293

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019268.01.T01	Q9FJK3	43.791	153	85	1	1	152	1	153	3.54E-31	117

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019268.01.T01	G7JJI5	95.597	159	7	0	1	159	1	159	5.34e-100	293

TFs/TRs:

Gene ID	Type	Classification
MsG0480019268.01.T01	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019268.01.T01	MTR_4g028720	95.597	159	7	0	1	159	1	159	1.35e-103	293
MsG0480019268.01.T01	MTR_7g011950	89.308	159	17	0	1	159	1	159	1.85e-97	278
MsG0480019268.01.T01	MTR_1g077320	83.648	159	26	0	1	159	1	159	4.33e-90	259
MsG0480019268.01.T01	MTR_5g047580	68.590	156	49	0	1	156	1	156	7.73e-75	221
MsG0480019268.01.T01	MTR_1g077390	68.153	157	50	0	1	157	1	157	1.41e-74	220
MsG0480019268.01.T01	MTR_1g084950	73.718	156	41	0	1	156	1	156	2.24e-74	219
MsG0480019268.01.T01	MTR_1g077300	72.327	159	42	1	1	159	1	157	1.85e-71	212
MsG0480019268.01.T01	MTR_5g047560	57.051	156	51	1	1	156	1	140	8.05e-56	172
MsG0480019268.01.T01	MTR_1g090697	52.903	155	73	0	1	155	1	155	2.00e-55	171
MsG0480019268.01.T01	MTR_1g090710	50.641	156	76	1	1	155	1	156	9.00e-53	165
MsG0480019268.01.T01	MTR_1g090783	51.634	153	74	0	3	155	2	154	8.75e-52	162
MsG0480019268.01.T01	MTR_4g032620	38.462	156	92	4	1	153	1	155	7.53e-38	129
MsG0480019268.01.T01	MTR_3g065100	45.223	157	85	1	1	156	1	157	5.87e-36	125
MsG0480019268.01.T01	MTR_3g466980	41.830	153	88	1	1	152	1	153	8.53e-36	124
MsG0480019268.01.T01	MTR_5g075380	35.256	156	100	1	1	155	1	156	2.16e-31	110
MsG0480019268.01.T01	MTR_4g063790	35.849	159	100	2	1	157	1	159	3.46e-31	110
MsG0480019268.01.T01	MTR_3g466830	38.854	157	95	1	3	158	2	158	1.15e-29	106
MsG0480019268.01.T01	MTR_8g036130	35.849	159	98	2	1	155	1	159	8.17e-29	106
MsG0480019268.01.T01	MTR_3g466890	38.312	154	94	1	3	155	2	155	6.10e-28	102
MsG0480019268.01.T01	MTR_4g031910	39.333	150	89	2	1	148	1	150	6.41e-28	103
MsG0480019268.01.T01	MTR_3g031240	32.704	159	105	2	1	157	1	159	1.97e-27	101
MsG0480019268.01.T01	MTR_4g032260	39.634	164	89	3	1	158	1	160	3.01e-27	100
MsG0480019268.01.T01	MTR_3g467080	32.298	161	107	2	1	159	1	161	4.41e-27	99.8
MsG0480019268.01.T01	MTR_2g016210	35.849	159	100	2	1	157	1	159	3.48e-25	97.1
MsG0480019268.01.T01	MTR_4g032290	36.646	161	100	2	1	159	1	161	1.27e-24	95.5
MsG0480019268.01.T01	MTR_2g035580	34.839	155	100	1	1	154	1	155	2.93e-23	90.1
MsG0480019268.01.T01	MTR_3g031100	31.847	157	105	2	1	155	1	157	5.60e-23	91.3
MsG0480019268.01.T01	MTR_2g035610	37.190	121	75	1	1	120	1	121	1.88e-22	87.0
MsG0480019268.01.T01	MTR_3g465410	33.588	131	85	2	29	157	1	131	1.23e-20	82.8
MsG0480019268.01.T01	MTR_3g466900	36.000	125	79	1	32	155	2	126	1.08e-17	74.7
MsG0480019268.01.T01	MTR_3g466930	34.400	125	81	1	32	155	2	126	1.40e-17	74.7
MsG0480019268.01.T01	MTR_4g019670	34.783	115	74	1	3	116	6	120	7.20e-17	77.0
MsG0480019268.01.T01	MTR_7g106510	37.037	108	67	1	3	109	6	113	1.01e-16	76.6
MsG0480019268.01.T01	MTR_4g028800	40.278	72	41	1	1	72	1	70	2.33e-15	67.0
MsG0480019268.01.T01	MTR_1g033790	41.837	98	56	1	62	158	20	117	1.31e-13	63.9
MsG0480019268.01.T01	MTR_1g033370	43.617	94	52	1	64	156	28	121	1.44e-13	63.9
MsG0480019268.01.T01	MTR_1g033930	39.796	98	58	1	62	158	20	117	1.60e-13	63.5
MsG0480019268.01.T01	MTR_7g055800	29.915	117	55	2	1	117	1	90	2.18e-12	60.1
MsG0480019268.01.T01	MTR_7g055790	37.615	109	51	2	1	109	59	150	1.51e-11	59.3
MsG0480019268.01.T01	MTR_3g093900	28.276	145	98	3	3	143	6	148	3.71e-11	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019268.01.T01	AT5G48670	43.791	153	85	1	1	152	1	153	3.61e-32	117
MsG0480019268.01.T01	AT3G05860	37.584	149	92	1	1	148	1	149	3.91e-29	106
MsG0480019268.01.T01	AT5G26630	38.125	160	98	1	1	159	1	160	1.10e-28	105
MsG0480019268.01.T01	AT3G05860	37.584	149	92	1	1	148	1	149	2.10e-28	105
MsG0480019268.01.T01	AT3G05860	37.584	149	92	1	1	148	1	149	3.60e-28	105
MsG0480019268.01.T01	AT1G65300	35.211	142	91	1	3	143	2	143	1.63e-25	99.0
MsG0480019268.01.T01	AT1G65330	35.374	147	94	1	3	148	2	148	3.47e-25	97.8
MsG0480019268.01.T01	AT5G26650	32.026	153	103	1	4	155	2	154	3.36e-22	91.3
MsG0480019268.01.T01	AT5G27960	31.373	153	104	1	4	155	2	154	5.09e-21	87.4
MsG0480019268.01.T01	AT1G22590	29.448	163	97	4	1	155	1	153	5.04e-20	81.6
MsG0480019268.01.T01	AT1G31630	28.387	155	109	2	3	156	2	155	2.37e-19	83.2
MsG0480019268.01.T01	AT5G27810	40.625	96	56	1	29	123	1	96	8.87e-19	77.4
MsG0480019268.01.T01	AT2G28700	32.215	149	100	1	1	148	1	149	1.84e-18	80.9
MsG0480019268.01.T01	AT5G26580	30.323	155	95	1	1	155	1	142	1.85e-16	75.5
MsG0480019268.01.T01	AT1G31640	30.000	150	103	2	3	151	2	150	2.18e-16	75.5
MsG0480019268.01.T01	AT2G40210	34.286	140	86	3	1	137	1	137	1.34e-14	70.1
MsG0480019268.01.T01	AT5G06500	31.013	158	107	2	1	157	1	157	2.58e-14	68.6

Find 26 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTGCAAAAGGAAACTTTCT+TGG	0.256030	4:+17472109	None:intergenic
CGCCTTTCTTGTTGAATCAT+TGG	0.287726	4:+17472326	None:intergenic
AGTTGAACATTCTTATGATT+TGG	0.299748	4:-17472029	MsG0480019268.01.T01:CDS
TGTTTGGCTTCCTTTAGATT+TGG	0.353502	4:+17472186	None:intergenic
ATACCTCTCAATCATCTGTT+TGG	0.362514	4:+17472170	None:intergenic
ACCTTTCTTCCACCGCTTGT+AGG	0.369888	4:+17472302	None:intergenic
CAGAGCTCATATATGAAAGA+TGG	0.456034	4:-17472147	MsG0480019268.01.T01:CDS
CTCCAATGATTCAACAAGAA+AGG	0.460238	4:-17472328	MsG0480019268.01.T01:CDS
TGTTTCTGATTTGAAAGACT+TGG	0.473410	4:-17471971	MsG0480019268.01.T01:CDS
TGCAAAAGATTACTAAAGCT+AGG	0.481563	4:-17472092	MsG0480019268.01.T01:CDS
GCAAAAGATTACTAAAGCTA+GGG	0.487712	4:-17472091	MsG0480019268.01.T01:CDS
ACCTACAAGCGGTGGAAGAA+AGG	0.494539	4:-17472303	MsG0480019268.01.T01:CDS
CAAAGTCATGGCTAGGAAGA+AGG	0.502352	4:-17472367	None:intergenic
AGAGGTGTGGCCAAATCTAA+AGG	0.522555	4:-17472196	MsG0480019268.01.T01:CDS
AGAGAACTCACTATTCTTTG+TGG	0.526508	4:-17472264	MsG0480019268.01.T01:CDS
TCGGAAATTACAACACATGC+TGG	0.533650	4:+17472237	None:intergenic
TCCATTTGATTCTAAAACAG+AGG	0.540902	4:-17472214	MsG0480019268.01.T01:CDS
GAAGGAAATTGATGACAAGA+TGG	0.549956	4:-17471926	MsG0480019268.01.T01:CDS
TCAGAAACAGTCACATCAGT+TGG	0.553395	4:+17471985	None:intergenic
AGAGCTCATATATGAAAGAT+GGG	0.560272	4:-17472146	MsG0480019268.01.T01:CDS
GAAGAAAGGTATCATCAAGA+AGG	0.595296	4:-17472289	MsG0480019268.01.T01:CDS
TACATTGCAAAGTCATGGCT+AGG	0.599615	4:-17472374	None:intergenic
AAGCCAAACAGATGATTGAG+AGG	0.630265	4:-17472173	MsG0480019268.01.T01:CDS
GAAAGGCGACCTACAAGCGG+TGG	0.634483	4:-17472311	MsG0480019268.01.T01:CDS
TTGATTCTAAAACAGAGGTG+TGG	0.661112	4:-17472209	MsG0480019268.01.T01:CDS
CAAGAAAGGCGACCTACAAG+CGG	0.669641	4:-17472314	MsG0480019268.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GTTGATTGAAAAAAATATGA+AGG	-	Chr4:17472319-17472338	MsG0480019268.01.T01:CDS	20.0%
!	AGTTGAACATTCTTATGATT+TGG	-	Chr4:17472234-17472253	MsG0480019268.01.T01:CDS	25.0%
!!	CTTTAGTAATCTTTTGCAAA+AGG	+	Chr4:17472169-17472188	None:intergenic	25.0%
!!!	TGTTTTAGAATCAAATGGAT+CGG	+	Chr4:17472048-17472067	None:intergenic	25.0%
	AGAGCTCATATATGAAAGAT+GGG	-	Chr4:17472117-17472136	MsG0480019268.01.T01:CDS	30.0%
	GCAAAAGATTACTAAAGCTA+GGG	-	Chr4:17472172-17472191	MsG0480019268.01.T01:CDS	30.0%
	TGCAAAAGATTACTAAAGCT+AGG	-	Chr4:17472171-17472190	MsG0480019268.01.T01:CDS	30.0%
	TTTGCAAAAGGAAACTTTCT+TGG	+	Chr4:17472157-17472176	None:intergenic	30.0%
!	TGTTTCTGATTTGAAAGACT+TGG	-	Chr4:17472292-17472311	MsG0480019268.01.T01:CDS	30.0%
!!	TCCATTTGATTCTAAAACAG+AGG	-	Chr4:17472049-17472068	MsG0480019268.01.T01:CDS	30.0%
!!!	ACCTCTGTTTTAGAATCAAA+TGG	+	Chr4:17472053-17472072	None:intergenic	30.0%
	AGAGAACTCACTATTCTTTG+TGG	-	Chr4:17471999-17472018	MsG0480019268.01.T01:CDS	35.0%
	ATACCTCTCAATCATCTGTT+TGG	+	Chr4:17472096-17472115	None:intergenic	35.0%
	CAGAGCTCATATATGAAAGA+TGG	-	Chr4:17472116-17472135	MsG0480019268.01.T01:CDS	35.0%
	CTCCAATGATTCAACAAGAA+AGG	-	Chr4:17471935-17471954	MsG0480019268.01.T01:CDS	35.0%
!	GAAGGAAATTGATGACAAGA+TGG	-	Chr4:17472337-17472356	MsG0480019268.01.T01:CDS	35.0%
!	TGTTTGGCTTCCTTTAGATT+TGG	+	Chr4:17472080-17472099	None:intergenic	35.0%
!!	GAAGAAAGGTATCATCAAGA+AGG	-	Chr4:17471974-17471993	MsG0480019268.01.T01:CDS	35.0%
!!	TTGATTCTAAAACAGAGGTG+TGG	-	Chr4:17472054-17472073	MsG0480019268.01.T01:CDS	35.0%
	AAGCCAAACAGATGATTGAG+AGG	-	Chr4:17472090-17472109	MsG0480019268.01.T01:CDS	40.0%
	CGCCTTTCTTGTTGAATCAT+TGG	+	Chr4:17471940-17471959	None:intergenic	40.0%
	TCAGAAACAGTCACATCAGT+TGG	+	Chr4:17472281-17472300	None:intergenic	40.0%
	TCGGAAATTACAACACATGC+TGG	+	Chr4:17472029-17472048	None:intergenic	40.0%
!!!	TTGGCAGCTTGTTGTTTTGT+AGG	+	Chr4:17472262-17472281	None:intergenic	40.0%
!	AGAGGTGTGGCCAAATCTAA+AGG	-	Chr4:17472067-17472086	MsG0480019268.01.T01:CDS	45.0%
	ACCTACAAGCGGTGGAAGAA+AGG	-	Chr4:17471960-17471979	MsG0480019268.01.T01:CDS	50.0%
	ACCTTTCTTCCACCGCTTGT+AGG	+	Chr4:17471964-17471983	None:intergenic	50.0%
	CAAGAAAGGCGACCTACAAG+CGG	-	Chr4:17471949-17471968	MsG0480019268.01.T01:CDS	50.0%
	GAAAGGCGACCTACAAGCGG+TGG	-	Chr4:17471952-17471971	MsG0480019268.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	17471903	17472382	17471903	ID=MsG0480019268.01;Name=MsG0480019268.01
Chr4	mRNA	17471903	17472382	17471903	ID=MsG0480019268.01.T01;Parent=MsG0480019268.01;Name=MsG0480019268.01.T01;_AED=0.47;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|159
Chr4	exon	17471903	17472382	17471903	ID=MsG0480019268.01.T01:exon:4571;Parent=MsG0480019268.01.T01
Chr4	CDS	17471903	17472382	17471903	ID=MsG0480019268.01.T01:cds;Parent=MsG0480019268.01.T01

Gene Sequence

>MsG0480019268.01.T01

ATGGCTAGGAAGAAGGTGAAAGTCGCTTTTATCTCCAATGATTCAACAAGAAAGGCGACCTACAAGCGGTGGAAGAAAGGTATCATCAAGAAGGTGAGAGAACTCACTATTCTTTGTGGTATTCCAGCATGTGTTGTAATTTCCGATCCATTTGATTCTAAAACAGAGGTGTGGCCAAATCTAAAGGAAGCCAAACAGATGATTGAGAGGTATCAGAGCTCATATATGAAAGATGGGAGAAAAAATGTGAACCAAGAAAGTTTCCTTTTGCAAAAGATTACTAAAGCTAGGGAGCAACTGAGAATACAAAGACACGACAATCATGAGAATGAGTTGAACATTCTTATGATTTGGTACCTACAAAACAACAAGCTGCCAACTGATGTGACTGTTTCTGATTTGAAAGACTTGGATAAGTTGATTGAAAAAAATATGAAGGAAATTGATGACAAGATGGCTTCACTTAGTTTATCAAACTGA

Protein sequence

>MsG0480019268.01.T01

MARKKVKVAFISNDSTRKATYKRWKKGIIKKVRELTILCGIPACVVISDPFDSKTEVWPNLKEAKQMIERYQSSYMKDGRKNVNQESFLLQKITKAREQLRIQRHDNHENELNILMIWYLQNNKLPTDVTVSDLKDLDKLIEKNMKEIDDKMASLSLSN*