AlfalfaGEDB | MsG0480019413.01

M. sativa cultivar ZhongmuNo.1 / MsG0480019413.01

KEGG: -
GO: GO:0004672 GO:0005524 GO:0006468
PFAM: PF00069

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019413.01.T01	XP_024629383.1	92.683	82	6	0	12	93	481	562	4.61E-42	157
MsG0480019413.01.T02	XP_024629383.1	83.333	120	20	0	7	126	481	600	8.06E-59	204
MsG0480019413.01.T03	XP_024629383.1	80.342	117	22	1	1	116	484	600	3.11E-53	189
MsG0480019413.01.T04	XP_024629383.1	80.531	113	21	1	1	112	488	600	6.35E-51	182
MsG0480019413.01.T05	XP_024629383.1	91.358	81	7	0	1	81	483	563	3.37E-41	154
MsG0480019413.01.T07	XP_024629383.1	92.593	81	6	0	1	81	481	561	2.11E-42	157
MsG0480019413.01.T08	XP_024629383.1	93.333	75	5	0	1	75	487	561	1.50E-38	146

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019413.01.T01	O81906	66.265	83	28	0	12	94	616	698	1.14E-29	114
MsG0480019413.01.T02	O81906	66.102	118	40	0	7	124	616	733	2.53E-44	157
MsG0480019413.01.T03	O64777	64.655	116	39	2	1	114	581	696	1.33E-40	146
MsG0480019413.01.T04	O64777	64.865	111	38	1	1	110	586	696	6.79E-40	144
MsG0480019413.01.T05	Q9LW83	65.823	79	27	0	2	80	617	695	1.77E-29	113
MsG0480019413.01.T07	O81906	66.667	81	27	0	1	81	616	696	7.11E-30	114
MsG0480019413.01.T08	O64782	68.056	72	23	0	2	73	582	653	1.75E-28	110

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019413.01.T01	A0A072U017	92.683	82	6	0	12	93	566	647	3.07e-42	157
MsG0480019413.01.T02	A0A072U017	83.333	120	20	0	7	126	566	685	1.19e-58	204
MsG0480019413.01.T03	A0A072U017	80.342	117	22	1	1	116	569	685	3.74e-53	189
MsG0480019413.01.T04	A0A072U017	80.531	113	21	1	1	112	573	685	7.69e-51	182
MsG0480019413.01.T07	A0A072U017	92.593	81	6	0	1	81	566	646	1.69e-42	157
MsG0480019413.01.T08	A0A072U017	93.333	75	5	0	1	75	572	646	9.19e-39	146

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019413.01.T01	MTR_8g442270	92.683	82	6	0	12	93	566	647	7.78e-46	157
MsG0480019413.01.T01	MTR_8g051640	87.952	83	10	0	12	94	535	617	5.13e-43	149
MsG0480019413.01.T01	MTR_8g442310	86.585	82	11	0	12	93	522	603	5.05e-42	146
MsG0480019413.01.T01	MTR_8g442370	85.542	83	12	0	12	94	556	638	6.70e-42	146
MsG0480019413.01.T01	MTR_8g051540	84.337	83	13	0	12	94	568	650	1.79e-41	144
MsG0480019413.01.T01	MTR_8g442340	79.518	83	17	0	12	94	509	591	4.09e-39	138
MsG0480019413.01.T01	MTR_8g052570	65.979	97	29	1	12	104	150	246	6.53e-39	134
MsG0480019413.01.T01	MTR_8g052517	65.979	97	29	1	12	104	150	246	6.53e-39	134
MsG0480019413.01.T01	MTR_8g052060	69.072	97	26	1	12	104	529	625	6.61e-39	137
MsG0480019413.01.T01	MTR_8g052120	68.041	97	27	1	12	104	543	639	1.68e-38	136

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019413.01.T01	AT4G21390	66.265	83	28	12	94	616	698	1.16e-30	114
MsG0480019413.01.T01	AT4G21390	67.073	82	27	12	93	616	697	1.30e-30	114
MsG0480019413.01.T01	AT1G67520	63.415	82	30	12	93	375	456	1.45e-30	113
MsG0480019413.01.T01	AT1G67520	63.415	82	30	12	93	375	456	1.45e-30	113
MsG0480019413.01.T01	AT1G67520	63.415	82	30	12	93	346	427	1.49e-30	113
MsG0480019413.01.T01	AT1G11330	63.855	83	30	12	94	611	693	1.69e-30	114
MsG0480019413.01.T01	AT1G11330	63.855	83	30	12	94	609	691	1.71e-30	113
MsG0480019413.01.T01	AT3G16030	64.634	82	29	12	93	614	695	1.85e-30	113
MsG0480019413.01.T01	AT3G16030	64.634	82	29	12	93	491	572	1.95e-30	113
MsG0480019413.01.T01	AT3G16030	64.634	82	29	12	93	635	716	2.00e-30	113

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Find 18 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGCGCTTTAGCATAATTGA+AGG	0.465352	4:-19806232	MsG0480019413.01.T01:CDS
AAGTCTGATGTGTATAGCTT+TGG	0.405512	4:-19805910	MsG0480019413.01.T02:CDS
ACTCCTTCCATGGCATACTC+CGG	0.570580	4:+19805943	None:intergenic
AGAAGAATATTTGATGAATT+AGG	0.308614	4:+19805833	None:intergenic
AGCAAGAATGTTTATGAAAC+AGG	0.354568	4:-19806086	MsG0480019413.01.T01:CDS
AGTCTGATGTGTATAGCTTT+GGG	0.446955	4:-19805909	MsG0480019413.01.T02:CDS
ATATGTCCCAACAATTCTGT+CGG	0.639659	4:+19806048	None:intergenic
ATTGTCTCCGGAGTATGCCA+TGG	0.608987	4:-19805950	MsG0480019413.01.T01:CDS
CAAATACCGACAGAATTGTT+GGG	0.541785	4:-19806054	MsG0480019413.01.T01:CDS
CTAGGAGAAAGCTGCTAGAT+TGG	0.384856	4:-19806258	MsG0480019413.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGTGACACTTAAAAAGGAA+CGG	-	Chr4:19806892-19806911	MsG0480019413.01.T01:intron	30.0%
AAGTGACACTTAAAAAGGAA+CGG	+	Chr4:19806911-19806892	None:intergenic	30.0%
AGCAAGAATGTTTATGAAAC+AGG	-	Chr4:19807129-19807148	MsG0480019413.01.T01:intron	30.0%
CTCTCCACAATAAATAACTA+AGG	+	Chr4:19806757-19806776	None:intergenic	30.0%
CTTGTAATTATGTCAAGTGT+AGG	-	Chr4:19806026-19806045	MsG0480019413.01.T01:intron	30.0%
GTAACATTTATTGAGTGTCT+TGG	-	Chr4:19806851-19806870	MsG0480019413.01.T01:intron	30.0%
TATTAGGCTTCAAATCTCTA+TGG	+	Chr4:19807060-19807079	None:intergenic	30.0%
TCCAAAAGTGACACTTAAAA+AGG	+	Chr4:19806660-19806679	None:intergenic	30.0%
AAGCGCTTTAGCATAATTGA+AGG	-	Chr4:19806983-19807002	MsG0480019413.01.T01:intron	35.0%
AAGTCTGATGTGTATAGCTT+TGG	-	Chr4:19807305-19807324	MsG0480019413.01.T01:intron	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	19805789	19807448	19805789	ID=MsG0480019413.01;Name=MsG0480019413.01
Chr4	mRNA	19805934	19807448	19805934	ID=MsG0480019413.01.T01;Parent=MsG0480019413.01;Name=MsG0480019413.01.T01;_AED=0.50;_eAED=0.57;_QI=0\|0\|0\|1\|0\|0\|3\|0\|106
Chr4	exon	19806047	19806302	19806047	ID=MsG0480019413.01.T01:exon:8077;Parent=MsG0480019413.01.T01
Chr4	exon	19807431	19807448	19807431	ID=MsG0480019413.01.T01:exon:8076;Parent=MsG0480019413.01.T01
Chr4	CDS	19807431	19807448	19807431	ID=MsG0480019413.01.T01:cds;Parent=MsG0480019413.01.T01
Chr4	CDS	19806047	19806302	19806047	ID=MsG0480019413.01.T01:cds;Parent=MsG0480019413.01.T01
Chr4	CDS	19805934	19805980	19805934	ID=MsG0480019413.01.T01:cds;Parent=MsG0480019413.01.T01
Chr4	mRNA	19805789	19806305	19805789	ID=MsG0480019413.01.T02;Parent=MsG0480019413.01;Name=MsG0480019413.01.T02;_AED=0.50;_eAED=0.50;_QI=0\|0\|0\|1\|0\|0\|2\|0\|148
Chr4	exon	19805789	19805965	19805789	ID=MsG0480019413.01.T02:exon:8080;Parent=MsG0480019413.01.T02
Chr4	exon	19806036	19806305	19806036	ID=MsG0480019413.01.T02:exon:8079;Parent=MsG0480019413.01.T02
Chr4	CDS	19806036	19806305	19806036	ID=MsG0480019413.01.T02:cds;Parent=MsG0480019413.01.T02
Chr4	CDS	19805789	19805965	19805789	ID=MsG0480019413.01.T02:cds;Parent=MsG0480019413.01.T02
Chr4	mRNA	19805789	19806278	19805789	ID=MsG0480019413.01.T03;Parent=MsG0480019413.01;Name=MsG0480019413.01.T03;_AED=0.50;_eAED=0.50;_QI=0\|0\|0\|1\|0\|0\|2\|0\|138
Chr4	exon	19806060	19806278	19806060	ID=MsG0480019413.01.T03:exon:8081;Parent=MsG0480019413.01.T03
Chr4	CDS	19806060	19806278	19806060	ID=MsG0480019413.01.T03:cds;Parent=MsG0480019413.01.T03
Chr4	CDS	19805789	19805986	19805789	ID=MsG0480019413.01.T03:cds;Parent=MsG0480019413.01.T03
Chr4	mRNA	19805789	19806266	19805789	ID=MsG0480019413.01.T04;Parent=MsG0480019413.01;Name=MsG0480019413.01.T04;_AED=0.50;_eAED=0.50;_QI=0\|0\|0\|1\|0\|0\|2\|0\|134
Chr4	exon	19806060	19806266	19806060	ID=MsG0480019413.01.T04:exon:8083;Parent=MsG0480019413.01.T04
Chr4	CDS	19806060	19806266	19806060	ID=MsG0480019413.01.T04:cds;Parent=MsG0480019413.01.T04
Chr4	CDS	19805789	19805986	19805789	ID=MsG0480019413.01.T04:cds;Parent=MsG0480019413.01.T04
Chr4	mRNA	19805934	19806281	19805934	ID=MsG0480019413.01.T05;Parent=MsG0480019413.01;Name=MsG0480019413.01.T05;_AED=0.50;_eAED=0.57;_QI=0\|0\|0\|1\|0\|0\|2\|0\|93
Chr4	exon	19806047	19806281	19806047	ID=MsG0480019413.01.T05:exon:8084;Parent=MsG0480019413.01.T05
Chr4	CDS	19806047	19806281	19806047	ID=MsG0480019413.01.T05:cds;Parent=MsG0480019413.01.T05
Chr4	CDS	19805934	19805980	19805934	ID=MsG0480019413.01.T05:cds;Parent=MsG0480019413.01.T05
Chr4	mRNA	19805934	19806101	19805934	ID=MsG0480019413.01.T06;Parent=MsG0480019413.01;Name=MsG0480019413.01.T06;_AED=0.50;_eAED=0.71;_QI=0\|0\|0\|1\|0\|0\|2\|0\|33
Chr4	exon	19806047	19806101	19806047	ID=MsG0480019413.01.T06:exon:8085;Parent=MsG0480019413.01.T06
Chr4	CDS	19806047	19806101	19806047	ID=MsG0480019413.01.T06:cds;Parent=MsG0480019413.01.T06
Chr4	CDS	19805934	19805980	19805934	ID=MsG0480019413.01.T06:cds;Parent=MsG0480019413.01.T06
Chr4	mRNA	19806003	19806287	19806003	ID=MsG0480019413.01.T07;Parent=MsG0480019413.01;Name=MsG0480019413.01.T07;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|94
Chr4	exon	19806003	19806287	19806003	ID=MsG0480019413.01.T07:exon:8086;Parent=MsG0480019413.01.T07
Chr4	CDS	19806003	19806287	19806003	ID=MsG0480019413.01.T07:cds;Parent=MsG0480019413.01.T07
Chr4	mRNA	19806003	19806269	19806003	ID=MsG0480019413.01.T08;Parent=MsG0480019413.01;Name=MsG0480019413.01.T08;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|88
Chr4	exon	19806003	19806269	19806003	ID=MsG0480019413.01.T08:exon:8087;Parent=MsG0480019413.01.T08
Chr4	CDS	19806003	19806269	19806003	ID=MsG0480019413.01.T08:cds;Parent=MsG0480019413.01.T08

Gene Sequence

>MsG0480019413.01.T05

ACTAGGAGAAAGCTGCTAGATTGGAATAAGCGCTTTAGCATAATTGAAGGAATCGCTCAAAGATTACTATATCTTCATAAGTACTCAAGACTCAGAATTATCCATAGAGATTTGAAGCCTAATAACATACTCCTTGATGAAAGCATGAACCCGAAAAATTCTGATTTTGGTGTAGCAAGAATGTTTATGAAACAGGAATTCGAGGCAAATACCGACAGAATTGTTGGGACATATACATATTTTATTGTCTCCGGAGTATGCCATGGAAGGAGTTTTTTCTAA

>MsG0480019413.01.T08

CTGCTAGATTGGAATAAGCGCTTTAGCATAATTGAAGGAATCGCTCAAAGATTACTATATCTTCATAAGTACTCAAGACTCAGAATTATCCATAGAGATTTGAAGCCTAATAACATACTCCTTGATGAAAGCATGAACCCGAAAAATTCTGATTTTGGTGTAGCAAGAATGTTTATGAAACAGGAATTCGAGGCAAATACCGACAGAATTGTTGGGACATATAGTGAGTTTCCTGTTTTTTTTCAAGCTTTCAATTCTGAAAACTAA

>MsG0480019413.01.T07

GATTCTACTAGGAGAAAGCTGCTAGATTGGAATAAGCGCTTTAGCATAATTGAAGGAATCGCTCAAAGATTACTATATCTTCATAAGTACTCAAGACTCAGAATTATCCATAGAGATTTGAAGCCTAATAACATACTCCTTGATGAAAGCATGAACCCGAAAAATTCTGATTTTGGTGTAGCAAGAATGTTTATGAAACAGGAATTCGAGGCAAATACCGACAGAATTGTTGGGACATATAGTGAGTTTCCTGTTTTTTTTCAAGCTTTCAATTCTGAAAACTAA

>MsG0480019413.01.T03

AGGAGAAAGCTGCTAGATTGGAATAAGCGCTTTAGCATAATTGAAGGAATCGCTCAAAGATTACTATATCTTCATAAGTACTCAAGACTCAGAATTATCCATAGAGATTTGAAGCCTAATAACATACTCCTTGATGAAAGCATGAACCCGAAAAATTCTGATTTTGGTGTAGCAAGAATGTTTATGAAACAGGAATTCGAGGCAAATACCGACAGAATTGATGAGCATATTTTATTGTCTCCGGAGTATGCCATGGAAGGAGTTTTTTCTAAGAAGTCTGATGTGTATAGCTTTGGGGTGTTACTCCTTGAACATGATTTTAACAAAAAGTCAGCTAGCTTCTTTGTCTTCCTAATTCATCAAATATTCTTCTACCATTTGAATCCTCCTGCAAAAACCAAAGAAAAAAGTCAATAA

>MsG0480019413.01.T01

ATGCTGTTAATACCAAAAGGGGGAGGGTATATAGATTCTACTAGGAGAAAGCTGCTAGATTGGAATAAGCGCTTTAGCATAATTGAAGGAATCGCTCAAAGATTACTATATCTTCATAAGTACTCAAGACTCAGAATTATCCATAGAGATTTGAAGCCTAATAACATACTCCTTGATGAAAGCATGAACCCGAAAAATTCTGATTTTGGTGTAGCAAGAATGTTTATGAAACAGGAATTCGAGGCAAATACCGACAGAATTGTTGGGACATATACATATTTTATTGTCTCCGGAGTATGCCATGGAAGGAGTTTTTTCTAA

>MsG0480019413.01.T06

ATGTTTATGAAACAGGAATTCGAGGCAAATACCGACAGAATTGTTGGGACATATACATATTTTATTGTCTCCGGAGTATGCCATGGAAGGAGTTTTTTCTAA

>MsG0480019413.01.T02

TGGGGGGGAGGGTATATAGATTCTACTAGGAGAAAGCTGCTAGATTGGAATAAGCGCTTTAGCATAATTGAAGGAATCGCTCAAAGATTACTATATCTTCATAAGTACTCAAGACTCAGAATTATCCATAGAGATTTGAAGCCTAATAACATACTCCTTGATGAAAGCATGAACCCGAAAAATTCTGATTTTGGTGTAGCAAGAATGTTTATGAAACAGGAATTCGAGGCAAATACCGACAGAATTGTTGGGACATATAGTGAGTTTCCTCCGGAGTATGCCATGGAAGGAGTTTTTTCTAAGAAGTCTGATGTGTATAGCTTTGGGGTGTTACTCCTTGAACATGATTTTAACAAAAAGTCAGCTAGCTTCTTTGTCTTCCTAATTCATCAAATATTCTTCTACCATTTGAATCCTCCTGCAAAAACCAAAGAAAAAAGTCAATAA

>MsG0480019413.01.T04

CTAGATTGGAATAAGCGCTTTAGCATAATTGAAGGAATCGCTCAAAGATTACTATATCTTCATAAGTACTCAAGACTCAGAATTATCCATAGAGATTTGAAGCCTAATAACATACTCCTTGATGAAAGCATGAACCCGAAAAATTCTGATTTTGGTGTAGCAAGAATGTTTATGAAACAGGAATTCGAGGCAAATACCGACAGAATTGATGAGCATATTTTATTGTCTCCGGAGTATGCCATGGAAGGAGTTTTTTCTAAGAAGTCTGATGTGTATAGCTTTGGGGTGTTACTCCTTGAACATGATTTTAACAAAAAGTCAGCTAGCTTCTTTGTCTTCCTAATTCATCAAATATTCTTCTACCATTTGAATCCTCCTGCAAAAACCAAAGAAAAAAGTCAATAA

Protein sequence

>MsG0480019413.01.T05

TRRKLLDWNKRFSIIEGIAQRLLYLHKYSRLRIIHRDLKPNNILLDESMNPKNSDFGVARMFMKQEFEANTDRIVGTYTYFIVSGVCHGRSFF*

>MsG0480019413.01.T08

LLDWNKRFSIIEGIAQRLLYLHKYSRLRIIHRDLKPNNILLDESMNPKNSDFGVARMFMKQEFEANTDRIVGTYSEFPVFFQAFNSEN*

>MsG0480019413.01.T07

DSTRRKLLDWNKRFSIIEGIAQRLLYLHKYSRLRIIHRDLKPNNILLDESMNPKNSDFGVARMFMKQEFEANTDRIVGTYSEFPVFFQAFNSEN*

>MsG0480019413.01.T03

RRKLLDWNKRFSIIEGIAQRLLYLHKYSRLRIIHRDLKPNNILLDESMNPKNSDFGVARMFMKQEFEANTDRIDEHILLSPEYAMEGVFSKKSDVYSFGVLLLEHDFNKKSASFFVFLIHQIFFYHLNPPAKTKEKSQ*

>MsG0480019413.01.T01

MLLIPKGGGYIDSTRRKLLDWNKRFSIIEGIAQRLLYLHKYSRLRIIHRDLKPNNILLDESMNPKNSDFGVARMFMKQEFEANTDRIVGTYTYFIVSGVCHGRSFF*

>MsG0480019413.01.T06

MFMKQEFEANTDRIVGTYTYFIVSGVCHGRSFF*

>MsG0480019413.01.T02

WGGGYIDSTRRKLLDWNKRFSIIEGIAQRLLYLHKYSRLRIIHRDLKPNNILLDESMNPKNSDFGVARMFMKQEFEANTDRIVGTYSEFPPEYAMEGVFSKKSDVYSFGVLLLEHDFNKKSASFFVFLIHQIFFYHLNPPAKTKEKSQ*

>MsG0480019413.01.T04

LDWNKRFSIIEGIAQRLLYLHKYSRLRIIHRDLKPNNILLDESMNPKNSDFGVARMFMKQEFEANTDRIDEHILLSPEYAMEGVFSKKSDVYSFGVLLLEHDFNKKSASFFVFLIHQIFFYHLNPPAKTKEKSQ*