AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021035.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021035.01.T01 MTR_4g064730 89.939 328 15 2 1 326 1 312 3.35e-168 470
MsG0480021035.01.T01 MTR_7g089010 43.534 232 76 5 8 223 5 197 7.18e-43 150
MsG0480021035.01.T01 MTR_4g121020 66.176 68 23 0 126 193 106 173 4.83e-25 105
MsG0480021035.01.T01 MTR_4g121020 66.176 68 23 0 126 193 210 277 5.61e-25 105
MsG0480021035.01.T01 MTR_4g121020 66.176 68 23 0 126 193 210 277 5.67e-25 105
MsG0480021035.01.T01 MTR_2g034960 49.057 106 47 1 126 224 206 311 3.59e-24 103
MsG0480021035.01.T01 MTR_7g117705 46.281 121 63 2 130 248 190 310 3.43e-23 99.8
MsG0480021035.01.T01 MTR_3g106220 49.485 97 46 1 130 226 197 290 3.73e-23 100
MsG0480021035.01.T01 MTR_2g450070 69.492 59 18 0 130 188 212 270 1.62e-22 98.6
MsG0480021035.01.T01 MTR_2g450070 69.492 59 18 0 130 188 212 270 1.84e-22 98.6
MsG0480021035.01.T01 MTR_4g131570 68.852 61 19 0 128 188 200 260 1.68e-21 95.5
MsG0480021035.01.T01 MTR_1g032570 57.143 77 29 1 113 189 117 189 2.37e-21 95.1
MsG0480021035.01.T01 MTR_1g032570 57.143 77 29 1 113 189 186 258 3.23e-21 94.7
MsG0480021035.01.T01 MTR_1g013170 44.954 109 45 2 130 227 190 294 8.13e-21 93.2
MsG0480021035.01.T01 MTR_4g131580 68.852 61 19 0 128 188 197 257 1.65e-20 92.4
MsG0480021035.01.T01 MTR_8g079940 51.685 89 41 2 113 200 190 277 1.56e-19 89.7
MsG0480021035.01.T01 MTR_0450s0040 39.200 125 67 2 128 246 166 287 1.86e-19 88.2
MsG0480021035.01.T01 MTR_3g102600 58.333 60 25 0 130 189 82 141 4.03e-19 84.0
MsG0480021035.01.T01 MTR_1g013180 51.316 76 37 0 130 205 341 416 4.46e-19 88.6
MsG0480021035.01.T01 MTR_3g102600 58.333 60 25 0 130 189 131 190 5.09e-19 84.7
MsG0480021035.01.T01 MTR_7g026400 53.125 64 30 0 124 187 150 213 1.37e-18 87.0
MsG0480021035.01.T01 MTR_4g131600 57.812 64 27 0 126 189 196 259 2.41e-18 86.3
MsG0480021035.01.T01 MTR_3g086100 36.000 150 85 4 102 247 105 247 1.32e-17 83.6
MsG0480021035.01.T01 MTR_3g086100 36.000 150 85 4 102 247 176 318 1.43e-17 84.0
MsG0480021035.01.T01 MTR_4g021760 33.951 162 89 5 110 258 146 302 6.09e-17 80.5
MsG0480021035.01.T01 MTR_4g098870 37.500 120 64 1 76 184 244 363 2.44e-16 80.1
MsG0480021035.01.T01 MTR_4g098870 37.500 120 64 1 76 184 244 363 2.48e-16 80.1
MsG0480021035.01.T01 MTR_4g098870 37.500 120 64 1 76 184 283 402 2.90e-16 79.7
MsG0480021035.01.T01 MTR_4g098870 37.500 120 64 1 76 184 264 383 3.26e-16 79.7
MsG0480021035.01.T01 MTR_4g098870 37.500 120 64 1 76 184 264 383 3.40e-16 79.7
MsG0480021035.01.T01 MTR_3g102590 51.389 72 33 1 130 199 142 213 4.10e-16 77.0
MsG0480021035.01.T01 MTR_5g014040 53.333 60 28 0 130 189 310 369 2.80e-15 77.0
MsG0480021035.01.T01 MTR_2g090675 43.038 79 43 1 130 206 160 238 2.24e-14 73.9
MsG0480021035.01.T01 MTR_4g086835 49.206 63 32 0 127 189 270 332 3.20e-14 73.2
MsG0480021035.01.T01 MTR_4g078810 44.928 69 38 0 130 198 157 225 3.46e-14 73.2
MsG0480021035.01.T01 MTR_4g021790 47.826 69 36 0 120 188 157 225 8.33e-14 70.9
MsG0480021035.01.T01 MTR_5g041350 38.679 106 53 4 128 227 12 111 2.70e-13 70.1
MsG0480021035.01.T01 MTR_5g014040 49.231 65 28 1 130 189 270 334 2.73e-13 70.9
MsG0480021035.01.T01 MTR_3g102600 50.943 53 26 0 130 182 82 134 3.06e-13 66.6
MsG0480021035.01.T01 MTR_5g014040 49.231 65 28 1 130 189 310 374 3.21e-13 70.5
MsG0480021035.01.T01 MTR_3g102600 50.943 53 26 0 130 182 131 183 3.55e-13 67.4
MsG0480021035.01.T01 MTR_5g017980 48.333 60 31 0 130 189 191 250 5.28e-13 68.9
MsG0480021035.01.T01 MTR_6g444980 52.174 69 31 2 117 184 22 89 5.33e-13 69.3
MsG0480021035.01.T01 MTR_5g017980 48.333 60 31 0 130 189 192 251 5.58e-13 68.9
MsG0480021035.01.T01 MTR_6g444980 52.174 69 31 2 117 184 22 89 6.41e-13 69.3
MsG0480021035.01.T01 MTR_4g086835 48.438 64 32 1 127 189 270 333 6.89e-13 69.3
MsG0480021035.01.T01 MTR_1g090670 60.000 55 21 1 131 184 44 98 7.14e-13 68.6
MsG0480021035.01.T01 MTR_4g113140 40.404 99 52 3 119 210 218 316 7.89e-13 69.3
MsG0480021035.01.T01 MTR_7g098250 34.127 126 76 3 65 183 190 315 8.70e-13 69.3
MsG0480021035.01.T01 MTR_8g105600 56.140 57 25 0 134 190 190 246 9.30e-13 69.3
MsG0480021035.01.T01 MTR_2g027800 52.055 73 29 2 131 197 39 111 9.99e-13 68.2
MsG0480021035.01.T01 MTR_2g027800 60.000 55 21 1 131 184 39 93 1.27e-12 67.8
MsG0480021035.01.T01 MTR_2g086450 55.172 58 25 1 128 184 42 99 1.67e-12 68.2
MsG0480021035.01.T01 MTR_1g093080 43.243 74 41 1 127 199 207 280 1.93e-12 67.8
MsG0480021035.01.T01 MTR_4g021855 49.231 65 32 1 125 188 161 225 2.60e-12 67.0
MsG0480021035.01.T01 MTR_1g080330 50.685 73 34 2 112 183 157 228 2.77e-12 66.6
MsG0480021035.01.T01 MTR_1g080330 49.315 73 35 2 112 183 157 228 2.94e-12 67.4
MsG0480021035.01.T01 MTR_6g032990 47.222 72 36 2 128 198 39 109 3.38e-12 67.0
MsG0480021035.01.T01 MTR_7g069660 49.315 73 36 1 112 183 52 124 4.72e-12 66.6
MsG0480021035.01.T01 MTR_7g115530 55.556 54 23 1 131 183 218 271 5.99e-12 65.9
MsG0480021035.01.T01 MTR_6g045327 48.276 58 30 0 130 187 171 228 6.28e-12 66.2
MsG0480021035.01.T01 MTR_5g027440 54.545 55 24 1 131 184 25 79 8.14e-12 65.1
MsG0480021035.01.T01 MTR_2g027860 48.611 72 35 2 131 201 49 119 8.66e-12 65.5
MsG0480021035.01.T01 MTR_7g115530 55.556 54 23 1 131 183 218 271 8.72e-12 65.5
MsG0480021035.01.T01 MTR_7g115530 55.556 54 23 1 131 183 218 271 9.52e-12 65.9
MsG0480021035.01.T01 MTR_7g115530 55.556 54 23 1 131 183 218 271 1.52e-11 65.1
MsG0480021035.01.T01 MTR_5g017980 47.541 61 31 1 130 189 192 252 1.56e-11 64.7
MsG0480021035.01.T01 MTR_5g054300 42.105 76 43 1 130 204 197 272 1.61e-11 65.1
MsG0480021035.01.T01 MTR_1g053830 50.943 53 25 1 133 184 20 72 2.12e-11 60.1
MsG0480021035.01.T01 MTR_6g453220 63.830 47 17 0 148 194 380 426 3.42e-11 64.3
MsG0480021035.01.T01 MTR_0223s0040 49.231 65 32 1 120 183 7 71 3.75e-11 63.2
MsG0480021035.01.T01 MTR_3g102590 54.902 51 23 0 130 180 142 192 5.08e-11 61.6
MsG0480021035.01.T01 MTR_1g112370 50.794 63 30 1 122 183 13 75 5.36e-11 63.2
MsG0480021035.01.T01 MTR_4g081710 50.000 58 28 1 128 184 14 71 5.50e-11 63.2
MsG0480021035.01.T01 MTR_2g023580 48.148 81 39 3 105 183 29 108 7.12e-11 62.0
MsG0480021035.01.T01 MTR_2g084230 53.125 64 29 1 121 183 87 150 9.05e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021035.01.T01 AT3G46640 57.021 235 75 4 1 215 1 229 5.75e-78 241
MsG0480021035.01.T01 AT3G46640 57.021 235 75 4 1 215 1 229 5.75e-78 241
MsG0480021035.01.T01 AT3G46640 57.021 235 75 4 1 215 1 229 2.94e-77 239
MsG0480021035.01.T01 AT5G59570 54.331 254 91 8 11 250 25 267 4.20e-73 228
MsG0480021035.01.T01 AT5G59570 54.331 254 91 8 11 250 25 267 4.20e-73 228
MsG0480021035.01.T01 AT3G10760 47.596 208 80 5 8 210 5 188 3.60e-39 141
MsG0480021035.01.T01 AT5G05090 81.176 85 15 1 127 210 77 161 3.15e-38 137
MsG0480021035.01.T01 AT2G40970 72.340 94 17 1 126 210 100 193 4.50e-38 136
MsG0480021035.01.T01 AT3G16857 52.128 94 40 1 100 193 141 229 2.25e-24 103
MsG0480021035.01.T01 AT3G16857 52.128 94 40 1 100 193 211 299 2.75e-24 103
MsG0480021035.01.T01 AT3G16857 52.128 94 40 1 100 193 211 299 2.98e-24 103
MsG0480021035.01.T01 AT2G25180 39.264 163 78 4 32 194 117 258 4.52e-22 97.4
MsG0480021035.01.T01 AT2G01760 57.895 76 29 1 130 202 134 209 7.84e-22 94.4
MsG0480021035.01.T01 AT5G58080 55.696 79 30 1 128 201 191 269 1.07e-21 96.3
MsG0480021035.01.T01 AT2G01760 57.895 76 29 1 130 202 199 274 2.17e-21 94.0
MsG0480021035.01.T01 AT2G01760 57.895 76 29 1 130 202 227 302 3.83e-21 93.6
MsG0480021035.01.T01 AT4G31920 44.792 96 50 1 130 225 182 274 1.35e-18 87.0
MsG0480021035.01.T01 AT5G44190 50.000 74 35 1 130 203 146 217 1.97e-16 79.7
MsG0480021035.01.T01 AT2G20570 33.544 158 99 2 41 196 65 218 2.08e-16 80.1
MsG0480021035.01.T01 AT5G44190 50.000 74 35 1 130 203 146 217 2.14e-16 79.3
MsG0480021035.01.T01 AT5G07210 46.053 76 39 1 119 192 210 285 7.91e-16 78.6
MsG0480021035.01.T01 AT5G07210 46.053 76 39 1 119 192 218 293 8.05e-16 78.6
MsG0480021035.01.T01 AT4G18020 30.275 218 120 6 84 293 242 435 1.91e-15 77.4
MsG0480021035.01.T01 AT4G18020 30.275 218 120 6 84 293 242 435 1.91e-15 77.4
MsG0480021035.01.T01 AT4G18020 30.275 218 120 6 84 293 242 435 1.91e-15 77.4
MsG0480021035.01.T01 AT4G18020 30.275 218 120 6 84 293 242 435 1.91e-15 77.4
MsG0480021035.01.T01 AT4G18020 30.275 218 120 6 84 293 242 435 1.91e-15 77.4
MsG0480021035.01.T01 AT4G18020 30.275 218 120 6 84 293 242 435 1.91e-15 77.4
MsG0480021035.01.T01 AT4G18020 30.275 218 120 6 84 293 242 435 3.46e-15 76.6
MsG0480021035.01.T01 AT4G18020 30.275 218 120 6 84 293 242 435 3.46e-15 76.6
MsG0480021035.01.T01 AT4G18020 30.275 218 120 6 84 293 242 435 3.46e-15 76.6
MsG0480021035.01.T01 AT4G18020 30.275 218 120 6 84 293 242 435 3.46e-15 76.6
MsG0480021035.01.T01 AT4G18020 30.275 218 120 6 84 293 242 435 3.46e-15 76.6
MsG0480021035.01.T01 AT2G20570 36.885 122 68 2 80 196 117 234 4.97e-15 75.9
MsG0480021035.01.T01 AT1G67710 42.857 98 49 3 97 190 94 188 6.37e-15 75.5
MsG0480021035.01.T01 AT1G67710 42.857 98 49 3 97 190 85 179 6.76e-15 75.5
MsG0480021035.01.T01 AT1G67710 42.857 98 49 3 97 190 159 253 1.07e-14 75.1
MsG0480021035.01.T01 AT3G12730 52.055 73 33 2 113 184 6 77 1.92e-14 72.0
MsG0480021035.01.T01 AT2G27070 45.679 81 41 2 130 207 224 304 2.65e-14 73.9
MsG0480021035.01.T01 AT3G04030 49.275 69 34 1 128 196 42 109 8.22e-14 72.0
MsG0480021035.01.T01 AT1G79430 53.623 69 30 2 117 184 21 88 1.09e-13 71.6
MsG0480021035.01.T01 AT5G45580 56.364 55 23 1 131 184 7 61 4.33e-13 68.6
MsG0480021035.01.T01 AT3G04030 50.000 70 33 2 128 196 42 110 4.54e-13 69.7
MsG0480021035.01.T01 AT5G45580 56.364 55 23 1 131 184 23 77 5.38e-13 68.6
MsG0480021035.01.T01 AT3G04030 50.000 70 33 2 128 196 42 110 5.53e-13 69.7
MsG0480021035.01.T01 AT5G18240 51.429 70 32 2 128 196 42 110 6.51e-13 69.3
MsG0480021035.01.T01 AT5G18240 51.429 70 32 2 128 196 42 110 6.51e-13 69.3
MsG0480021035.01.T01 AT3G24120 58.182 55 22 1 131 184 41 95 6.54e-13 68.6
MsG0480021035.01.T01 AT3G24120 58.182 55 22 1 131 184 41 95 6.62e-13 68.6
MsG0480021035.01.T01 AT5G18240 51.429 70 32 2 128 196 42 110 7.04e-13 69.3
MsG0480021035.01.T01 AT5G18240 51.429 70 32 2 128 196 42 110 7.42e-13 69.3
MsG0480021035.01.T01 AT5G18240 51.429 70 32 2 128 196 42 110 7.42e-13 69.3
MsG0480021035.01.T01 AT4G13640 58.182 55 22 1 131 184 37 91 7.78e-13 68.2
MsG0480021035.01.T01 AT4G13640 58.182 55 22 1 131 184 37 91 8.38e-13 68.2
MsG0480021035.01.T01 AT1G13300 42.529 87 46 3 130 212 180 266 8.69e-13 68.6
MsG0480021035.01.T01 AT3G62670 55.932 59 21 2 130 183 210 268 8.79e-13 68.9
MsG0480021035.01.T01 AT2G03500 47.692 65 33 1 125 188 227 291 1.20e-12 68.6
MsG0480021035.01.T01 AT2G01060 45.055 91 45 3 118 203 2 92 1.84e-12 67.0
MsG0480021035.01.T01 AT3G13040 55.357 56 24 1 130 184 240 295 1.09e-11 65.9
MsG0480021035.01.T01 AT3G13040 55.357 56 24 1 130 184 240 295 1.09e-11 65.9
MsG0480021035.01.T01 AT3G13040 55.357 56 24 1 130 184 240 295 1.09e-11 65.9
MsG0480021035.01.T01 AT2G38300 40.196 102 57 2 107 207 30 128 1.26e-11 65.1
MsG0480021035.01.T01 AT1G49560 44.118 68 37 1 122 188 183 250 2.20e-11 64.3
MsG0480021035.01.T01 AT1G25550 48.333 60 30 1 130 188 208 267 3.72e-11 63.9
MsG0480021035.01.T01 AT5G49240 50.980 51 25 0 130 180 222 272 4.76e-11 63.2
MsG0480021035.01.T01 AT3G25790 40.449 89 49 3 135 219 200 288 7.01e-11 63.2

Find 93 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATGATGCATCATATTAAA+TGG 0.267208 4:-52452357 None:intergenic
GGAGTGAGATCGTGGAGGTT+AGG 0.288087 4:-52451517 None:intergenic
CATTTAATATGATGCATCAT+AGG 0.291545 4:+52452358 MsG0480021035.01.T01:CDS
GATGAGTTTGCCTCCTCCTT+CGG 0.309025 4:+52452308 MsG0480021035.01.T01:CDS
GAGTGAGATCGTGGAGGTTA+GGG 0.316953 4:-52451516 None:intergenic
CGTTTATTTATTACTATCAT+TGG 0.321692 4:-52452421 None:intergenic
ACATCATCCACATGGGCATA+TGG 0.344716 4:+52452284 MsG0480021035.01.T01:CDS
GTGGATGTTGTTGCTCATCT+TGG 0.347586 4:+52451892 MsG0480021035.01.T01:CDS
ATGAGTTTGCCTCCTCCTTC+GGG 0.352579 4:+52452309 MsG0480021035.01.T01:CDS
CTCGAAGGTGGTGGTGGATA+AGG 0.394424 4:-52452228 None:intergenic
GAACAACGGAGACGATAAAA+AGG 0.405625 4:+52451830 MsG0480021035.01.T01:CDS
GTCTTCTACTTCGCTGGACA+CGG 0.409549 4:-52451695 None:intergenic
CATCATCCACATGGGCATAT+GGG 0.413116 4:+52452285 MsG0480021035.01.T01:CDS
TCAAATGGAGGGCATGGAAA+TGG 0.414953 4:+52452150 MsG0480021035.01.T01:CDS
AAGAGAGTACGGAGAGGCTC+CGG 0.421773 4:-52451592 None:intergenic
TCAAATGCTAATCATTCAAA+TGG 0.429533 4:+52452135 MsG0480021035.01.T01:CDS
TTCAACGTCTTCTACTTCGC+TGG 0.432464 4:-52451701 None:intergenic
TGATTGTTCCGGAGTTGCTT+CGG 0.434817 4:-52451654 None:intergenic
TGAGGATGAGAATGTGGATT+CGG 0.435504 4:-52452390 None:intergenic
TGGATGTTGTTGCTCATCTT+GGG 0.442275 4:+52451893 MsG0480021035.01.T01:CDS
CCGCAGAGTTTGCATGAAAC+CGG 0.443486 4:+52452087 MsG0480021035.01.T01:CDS
TTCCATGGCTTTGATCCCAC+CGG 0.445349 4:+52451543 MsG0480021035.01.T01:CDS
AGTGGAAGCTGAAGCAGATT+CGG 0.449529 4:+52451801 MsG0480021035.01.T01:CDS
ACGTGTAACGGAGTGGGAAA+AGG 0.453107 4:+52451489 MsG0480021035.01.T01:CDS
AGAGAGTACGGAGAGGCTCC+GGG 0.454039 4:-52451591 None:intergenic
GAACCCGAGCGAGATGGATC+AGG 0.456693 4:+52451733 MsG0480021035.01.T01:CDS
CTGAAAGCGGAAGCGAGCTC+CGG 0.460721 4:-52451562 None:intergenic
TGATGTCCCATACCAATCAT+AGG 0.460751 4:-52452267 None:intergenic
TCATGCAAACTCTGCGGCAC+AGG 0.465750 4:-52452081 None:intergenic
GATTTGATGAGTGATTGTTC+CGG 0.471479 4:-52451665 None:intergenic
ACAGCTACATAAAAGATTTG+TGG 0.472539 4:+52451873 MsG0480021035.01.T01:CDS
CGATCTCACTCCTCTTTCCA+TGG 0.482732 4:+52451528 MsG0480021035.01.T01:CDS
ATATGATGCATCATAGGGAT+TGG 0.484646 4:+52452364 MsG0480021035.01.T01:CDS
ATGAGAATGATTAGCAGAAC+CGG 0.486272 4:-52452106 None:intergenic
ATTTAATATGATGCATCATA+GGG 0.488329 4:+52452359 MsG0480021035.01.T01:CDS
ATGCAGTTGATGAATGTTGA+AGG 0.492393 4:+52451943 MsG0480021035.01.T01:CDS
TTCCGCTTTCAGCATCTCCC+CGG 0.493661 4:+52451573 MsG0480021035.01.T01:CDS
ATCATACTCGAAGGTGGTGG+TGG 0.507063 4:-52452234 None:intergenic
CGTGTAACGGAGTGGGAAAA+GGG 0.517852 4:+52451490 MsG0480021035.01.T01:CDS
TCTCTTCTCCGAAGCAACTC+CGG 0.518098 4:+52451646 MsG0480021035.01.T01:CDS
CGAGCGAGATGGATCAGGTT+CGG 0.519486 4:+52451738 MsG0480021035.01.T01:CDS
AAAGCGGAAGCGAGCTCCGG+TGG 0.521066 4:-52451559 None:intergenic
GACGAAGAACCCGAGCGAGA+TGG 0.525147 4:+52451727 MsG0480021035.01.T01:CDS
AATCATTCAAATGGAGGGCA+TGG 0.528339 4:+52452144 MsG0480021035.01.T01:CDS
AGATTCTGTTACAGAAGAAG+TGG 0.528651 4:+52451783 MsG0480021035.01.T01:CDS
CGAACCTGATCCATCTCGCT+CGG 0.534136 4:-52451737 None:intergenic
AAAAGATGATCAGAAGAAGA+AGG 0.541009 4:-52452048 None:intergenic
GAACCTGATCCATCTCGCTC+GGG 0.541572 4:-52451736 None:intergenic
ATGTCAATGCCGCCTATGAT+TGG 0.542928 4:+52452255 MsG0480021035.01.T01:CDS
GACATATGAGGATGAGAATG+TGG 0.546426 4:-52452396 None:intergenic
TCTTCTACTTCGCTGGACAC+GGG 0.550393 4:-52451694 None:intergenic
ACGAAGAACGTGTAACGGAG+TGG 0.550873 4:+52451482 MsG0480021035.01.T01:CDS
AATGCTAATCATTCAAATGG+AGG 0.554348 4:+52452138 MsG0480021035.01.T01:CDS
ATCATCCACATGGGCATATG+GGG 0.557841 4:+52452286 MsG0480021035.01.T01:CDS
CTAGAACCACCCGAAGGAGG+AGG 0.558990 4:-52452318 None:intergenic
CTATCATTGGAAGACATATG+AGG 0.559295 4:-52452408 None:intergenic
GGGATCAAAGCCATGGAAAG+AGG 0.559832 4:-52451538 None:intergenic
ATGCCGCCTATGATTGGTAT+GGG 0.560585 4:+52452261 MsG0480021035.01.T01:CDS
GGTTGACGTCGAAGAGAGTA+CGG 0.568518 4:-52451603 None:intergenic
CTCCGGTGGGATCAAAGCCA+TGG 0.569390 4:-52451545 None:intergenic
GTTCGGATTCAAGAAAACAT+AGG 0.571081 4:+52451755 MsG0480021035.01.T01:CDS
AATGCCGCCTATGATTGGTA+TGG 0.579585 4:+52452260 MsG0480021035.01.T01:CDS
ATGCTAATCATTCAAATGGA+GGG 0.580774 4:+52452139 MsG0480021035.01.T01:CDS
CTTTATTTGAAGAGAATGCA+AGG 0.583827 4:+52452009 MsG0480021035.01.T01:CDS
AACAAACTGAATGGGTGAAG+AGG 0.590082 4:+52451450 None:intergenic
GGTGGTGGTGGATAAGGCAT+AGG 0.595338 4:-52452222 None:intergenic
CTCCGGGGAGATGCTGAAAG+CGG 0.595726 4:-52451575 None:intergenic
TGGAAAGAGGAGTGAGATCG+TGG 0.601806 4:-52451525 None:intergenic
TTTATTTGAAGAGAATGCAA+GGG 0.602218 4:+52452010 MsG0480021035.01.T01:CDS
AAGAGAGAGTGTGTTGCGAG+AGG 0.612371 4:-52451629 None:intergenic
GACATCATACTCGAAGGTGG+TGG 0.615415 4:-52452237 None:intergenic
GAGCTAGAACCACCCGAAGG+AGG 0.617421 4:-52452321 None:intergenic
GGAGGGCATGGAAATGGACA+TGG 0.620162 4:+52452156 MsG0480021035.01.T01:CDS
GGCATTGACATCATACTCGA+AGG 0.620560 4:-52452243 None:intergenic
CTCATCCCCATATGCCCATG+TGG 0.624417 4:-52452291 None:intergenic
GAGAGTACGGAGAGGCTCCG+GGG 0.626074 4:-52451590 None:intergenic
AGTTTGCCTCCTCCTTCGGG+TGG 0.628086 4:+52452312 MsG0480021035.01.T01:CDS
CGAAGAACGTGTAACGGAGT+GGG 0.629837 4:+52451483 MsG0480021035.01.T01:CDS
TGTGGGGTCCACACCAACCT+CGG 0.635154 4:-52451853 None:intergenic
AAGCGGAAGCGAGCTCCGGT+GGG 0.636737 4:-52451558 None:intergenic
AACAGACGAAGAACGTGTAA+CGG 0.639303 4:+52451477 MsG0480021035.01.T01:CDS
GGATGAGAATGTGGATTCGG+AGG 0.643753 4:-52452387 None:intergenic
GGTATGGGACATCATCCACA+TGG 0.649470 4:+52452276 MsG0480021035.01.T01:CDS
AAAGAGGAGTGAGATCGTGG+AGG 0.675435 4:-52451522 None:intergenic
ATTGACATCATACTCGAAGG+TGG 0.684359 4:-52452240 None:intergenic
AGAGTGTGTTGCGAGAGGCG+CGG 0.693296 4:-52451624 None:intergenic
TAAGAGCTAGAACCACCCGA+AGG 0.715458 4:-52452324 None:intergenic
AGATTCGGCTGTTAGAACAA+CGG 0.719098 4:+52451816 MsG0480021035.01.T01:CDS
TCATCTTGGGATAAAGAACG+CGG 0.719383 4:+52451906 MsG0480021035.01.T01:CDS
GTATGGGACATCATCCACAT+GGG 0.727717 4:+52452277 MsG0480021035.01.T01:CDS
CCGGTTTCATGCAAACTCTG+CGG 0.740073 4:-52452087 None:intergenic
TGTCCCATACCAATCATAGG+CGG 0.741457 4:-52452264 None:intergenic
ACGTCGAAGAGAGTACGGAG+AGG 0.748301 4:-52451598 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAATATGATGCATCATA+GGG + Chr4:52452359-52452378 MsG0480021035.01.T01:CDS 20.0%
! CATTTAATATGATGCATCAT+AGG + Chr4:52452358-52452377 MsG0480021035.01.T01:CDS 25.0%
! CTATGATGCATCATATTAAA+TGG - Chr4:52452360-52452379 None:intergenic 25.0%
! TCAAATGCTAATCATTCAAA+TGG + Chr4:52452135-52452154 MsG0480021035.01.T01:CDS 25.0%
! TTTATTTGAAGAGAATGCAA+GGG + Chr4:52452010-52452029 MsG0480021035.01.T01:CDS 25.0%
AAAAGATGATCAGAAGAAGA+AGG - Chr4:52452051-52452070 None:intergenic 30.0%
AATGCTAATCATTCAAATGG+AGG + Chr4:52452138-52452157 MsG0480021035.01.T01:CDS 30.0%
ACAGCTACATAAAAGATTTG+TGG + Chr4:52451873-52451892 MsG0480021035.01.T01:CDS 30.0%
ATGCTAATCATTCAAATGGA+GGG + Chr4:52452139-52452158 MsG0480021035.01.T01:CDS 30.0%
! AAATCTTTTATGTAGCTGTG+GGG - Chr4:52451872-52451891 None:intergenic 30.0%
! ACAAATCTTTTATGTAGCTG+TGG - Chr4:52451874-52451893 None:intergenic 30.0%
! ATCAACTGCATAATCGTTTT+CGG - Chr4:52451934-52451953 None:intergenic 30.0%
! CAAATCTTTTATGTAGCTGT+GGG - Chr4:52451873-52451892 None:intergenic 30.0%
! CTTTATTTGAAGAGAATGCA+AGG + Chr4:52452009-52452028 MsG0480021035.01.T01:CDS 30.0%
AGATTCTGTTACAGAAGAAG+TGG + Chr4:52451783-52451802 MsG0480021035.01.T01:CDS 35.0%
ATATGATGCATCATAGGGAT+TGG + Chr4:52452364-52452383 MsG0480021035.01.T01:CDS 35.0%
ATGAGAATGATTAGCAGAAC+CGG - Chr4:52452109-52452128 None:intergenic 35.0%
CTATCATTGGAAGACATATG+AGG - Chr4:52452411-52452430 None:intergenic 35.0%
GATTTGATGAGTGATTGTTC+CGG - Chr4:52451668-52451687 None:intergenic 35.0%
GTTCGGATTCAAGAAAACAT+AGG + Chr4:52451755-52451774 MsG0480021035.01.T01:CDS 35.0%
!! ATGCAGTTGATGAATGTTGA+AGG + Chr4:52451943-52451962 MsG0480021035.01.T01:CDS 35.0%
AACAGACGAAGAACGTGTAA+CGG + Chr4:52451477-52451496 MsG0480021035.01.T01:CDS 40.0%
AATCATTCAAATGGAGGGCA+TGG + Chr4:52452144-52452163 MsG0480021035.01.T01:CDS 40.0%
AGATTCGGCTGTTAGAACAA+CGG + Chr4:52451816-52451835 MsG0480021035.01.T01:CDS 40.0%
ATTGACATCATACTCGAAGG+TGG - Chr4:52452243-52452262 None:intergenic 40.0%
GAACAACGGAGACGATAAAA+AGG + Chr4:52451830-52451849 MsG0480021035.01.T01:CDS 40.0%
GACATATGAGGATGAGAATG+TGG - Chr4:52452399-52452418 None:intergenic 40.0%
TCATCTTGGGATAAAGAACG+CGG + Chr4:52451906-52451925 MsG0480021035.01.T01:CDS 40.0%
TGAGGATGAGAATGTGGATT+CGG - Chr4:52452393-52452412 None:intergenic 40.0%
TGATGTCCCATACCAATCAT+AGG - Chr4:52452270-52452289 None:intergenic 40.0%
TGGATGTTGTTGCTCATCTT+GGG + Chr4:52451893-52451912 MsG0480021035.01.T01:CDS 40.0%
AATGCCGCCTATGATTGGTA+TGG + Chr4:52452260-52452279 MsG0480021035.01.T01:CDS 45.0%
ACATCATCCACATGGGCATA+TGG + Chr4:52452284-52452303 MsG0480021035.01.T01:CDS 45.0%
AGTGGAAGCTGAAGCAGATT+CGG + Chr4:52451801-52451820 MsG0480021035.01.T01:CDS 45.0%
ATCATCCACATGGGCATATG+GGG + Chr4:52452286-52452305 MsG0480021035.01.T01:CDS 45.0%
ATGCCGCCTATGATTGGTAT+GGG + Chr4:52452261-52452280 MsG0480021035.01.T01:CDS 45.0%
ATGTCAATGCCGCCTATGAT+TGG + Chr4:52452255-52452274 MsG0480021035.01.T01:CDS 45.0%
CATCATCCACATGGGCATAT+GGG + Chr4:52452285-52452304 MsG0480021035.01.T01:CDS 45.0%
GTATGGGACATCATCCACAT+GGG + Chr4:52452277-52452296 MsG0480021035.01.T01:CDS 45.0%
GTGGATGTTGTTGCTCATCT+TGG + Chr4:52451892-52451911 MsG0480021035.01.T01:CDS 45.0%
TGATTGTTCCGGAGTTGCTT+CGG - Chr4:52451657-52451676 None:intergenic 45.0%
TGTCCCATACCAATCATAGG+CGG - Chr4:52452267-52452286 None:intergenic 45.0%
TTCAACGTCTTCTACTTCGC+TGG - Chr4:52451704-52451723 None:intergenic 45.0%
! TCAAATGGAGGGCATGGAAA+TGG + Chr4:52452150-52452169 MsG0480021035.01.T01:CDS 45.0%
!! GGCATTGACATCATACTCGA+AGG - Chr4:52452246-52452265 None:intergenic 45.0%
AAAGAGGAGTGAGATCGTGG+AGG - Chr4:52451525-52451544 None:intergenic 50.0%
ATGAGTTTGCCTCCTCCTTC+GGG + Chr4:52452309-52452328 MsG0480021035.01.T01:CDS 50.0%
CCGCAGAGTTTGCATGAAAC+CGG + Chr4:52452087-52452106 MsG0480021035.01.T01:CDS 50.0%
CCGGTTTCATGCAAACTCTG+CGG - Chr4:52452090-52452109 None:intergenic 50.0%
CGATCTCACTCCTCTTTCCA+TGG + Chr4:52451528-52451547 MsG0480021035.01.T01:CDS 50.0%
GACATCATACTCGAAGGTGG+TGG - Chr4:52452240-52452259 None:intergenic 50.0%
GACGATAAAAAGGCCGAGGT+TGG + Chr4:52451840-52451859 MsG0480021035.01.T01:CDS 50.0%
GAGTGAGATCGTGGAGGTTA+GGG - Chr4:52451519-52451538 None:intergenic 50.0%
GATGAGTTTGCCTCCTCCTT+CGG + Chr4:52452308-52452327 MsG0480021035.01.T01:CDS 50.0%
GGATGAGAATGTGGATTCGG+AGG - Chr4:52452390-52452409 None:intergenic 50.0%
GGGATCAAAGCCATGGAAAG+AGG - Chr4:52451541-52451560 None:intergenic 50.0%
GGTATGGGACATCATCCACA+TGG + Chr4:52452276-52452295 MsG0480021035.01.T01:CDS 50.0%
GGTTGACGTCGAAGAGAGTA+CGG - Chr4:52451606-52451625 None:intergenic 50.0%
GTCTTCTACTTCGCTGGACA+CGG - Chr4:52451698-52451717 None:intergenic 50.0%
TAAGAGCTAGAACCACCCGA+AGG - Chr4:52452327-52452346 None:intergenic 50.0%
TCTCTTCTCCGAAGCAACTC+CGG + Chr4:52451646-52451665 MsG0480021035.01.T01:CDS 50.0%
TCTTCTACTTCGCTGGACAC+GGG - Chr4:52451697-52451716 None:intergenic 50.0%
TGGAAAGAGGAGTGAGATCG+TGG - Chr4:52451528-52451547 None:intergenic 50.0%
! AAGAGAGAGTGTGTTGCGAG+AGG - Chr4:52451632-52451651 None:intergenic 50.0%
! ACGAAGAACGTGTAACGGAG+TGG + Chr4:52451482-52451501 MsG0480021035.01.T01:CDS 50.0%
! ACGTGTAACGGAGTGGGAAA+AGG + Chr4:52451489-52451508 MsG0480021035.01.T01:CDS 50.0%
! ATCATACTCGAAGGTGGTGG+TGG - Chr4:52452237-52452256 None:intergenic 50.0%
! CGAAGAACGTGTAACGGAGT+GGG + Chr4:52451483-52451502 MsG0480021035.01.T01:CDS 50.0%
! CGTGTAACGGAGTGGGAAAA+GGG + Chr4:52451490-52451509 MsG0480021035.01.T01:CDS 50.0%
!! TAAAAAGGCCGAGGTTGGTG+TGG + Chr4:52451845-52451864 MsG0480021035.01.T01:CDS 50.0%
!! TTCCATGGCTTTGATCCCAC+CGG + Chr4:52451543-52451562 MsG0480021035.01.T01:CDS 50.0%
CGAACCTGATCCATCTCGCT+CGG - Chr4:52451740-52451759 None:intergenic 55.0%
CGAGCGAGATGGATCAGGTT+CGG + Chr4:52451738-52451757 MsG0480021035.01.T01:CDS 55.0%
CGGAGACGATAAAAAGGCCG+AGG + Chr4:52451836-52451855 MsG0480021035.01.T01:CDS 55.0%
CTCATCCCCATATGCCCATG+TGG - Chr4:52452294-52452313 None:intergenic 55.0%
GAACCTGATCCATCTCGCTC+GGG - Chr4:52451739-52451758 None:intergenic 55.0%
GGAGTGAGATCGTGGAGGTT+AGG - Chr4:52451520-52451539 None:intergenic 55.0%
TCATGCAAACTCTGCGGCAC+AGG - Chr4:52452084-52452103 None:intergenic 55.0%
TTCCGCTTTCAGCATCTCCC+CGG + Chr4:52451573-52451592 MsG0480021035.01.T01:CDS 55.0%
! AAGAGAGTACGGAGAGGCTC+CGG - Chr4:52451595-52451614 None:intergenic 55.0%
! ACGTCGAAGAGAGTACGGAG+AGG - Chr4:52451601-52451620 None:intergenic 55.0%
! GGAGGGCATGGAAATGGACA+TGG + Chr4:52452156-52452175 MsG0480021035.01.T01:CDS 55.0%
!! CTCGAAGGTGGTGGTGGATA+AGG - Chr4:52452231-52452250 None:intergenic 55.0%
!! GGTGGTGGTGGATAAGGCAT+AGG - Chr4:52452225-52452244 None:intergenic 55.0%
AGAGTGTGTTGCGAGAGGCG+CGG - Chr4:52451627-52451646 None:intergenic 60.0%
AGTTTGCCTCCTCCTTCGGG+TGG + Chr4:52452312-52452331 MsG0480021035.01.T01:CDS 60.0%
CTAGAACCACCCGAAGGAGG+AGG - Chr4:52452321-52452340 None:intergenic 60.0%
CTCCGGGGAGATGCTGAAAG+CGG - Chr4:52451578-52451597 None:intergenic 60.0%
CTCCGGTGGGATCAAAGCCA+TGG - Chr4:52451548-52451567 None:intergenic 60.0%
CTGAAAGCGGAAGCGAGCTC+CGG - Chr4:52451565-52451584 None:intergenic 60.0%
GAACCCGAGCGAGATGGATC+AGG + Chr4:52451733-52451752 MsG0480021035.01.T01:CDS 60.0%
GACGAAGAACCCGAGCGAGA+TGG + Chr4:52451727-52451746 MsG0480021035.01.T01:CDS 60.0%
GAGCTAGAACCACCCGAAGG+AGG - Chr4:52452324-52452343 None:intergenic 60.0%
TGTGGGGTCCACACCAACCT+CGG - Chr4:52451856-52451875 None:intergenic 60.0%
! AGAGAGTACGGAGAGGCTCC+GGG - Chr4:52451594-52451613 None:intergenic 60.0%
AAAGCGGAAGCGAGCTCCGG+TGG - Chr4:52451562-52451581 None:intergenic 65.0%
AAGCGGAAGCGAGCTCCGGT+GGG - Chr4:52451561-52451580 None:intergenic 65.0%
! GAGAGTACGGAGAGGCTCCG+GGG - Chr4:52451593-52451612 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 52451460 52452440 52451460 ID=MsG0480021035.01;Name=MsG0480021035.01
Chr4 mRNA 52451460 52452440 52451460 ID=MsG0480021035.01.T01;Parent=MsG0480021035.01;Name=MsG0480021035.01.T01;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|326
Chr4 exon 52451460 52452440 52451460 ID=MsG0480021035.01.T01:exon:16939;Parent=MsG0480021035.01.T01
Chr4 CDS 52451460 52452440 52451460 ID=MsG0480021035.01.T01:cds;Parent=MsG0480021035.01.T01
Gene Sequence

>MsG0480021035.01.T01

ATGGGTGAAGAGGTTCGAACAGACGAAGAACGTGTAACGGAGTGGGAAAAGGGTCTCCCTAACCTCCACGATCTCACTCCTCTTTCCATGGCTTTGATCCCACCGGAGCTCGCTTCCGCTTTCAGCATCTCCCCGGAGCCTCTCCGTACTCTCTTCGACGTCAACCGCGCCTCTCGCAACACACTCTCTCTTCTCCGAAGCAACTCCGGAACAATCACTCATCAAATCAATCAACCCGTGTCCAGCGAAGTAGAAGACGTTGAAGAAGACGAAGAACCCGAGCGAGATGGATCAGGTTCGGATTCAAGAAAACATAGGAAAATAGATTCTGTTACAGAAGAAGTGGAAGCTGAAGCAGATTCGGCTGTTAGAACAACGGAGACGATAAAAAGGCCGAGGTTGGTGTGGACCCCACAGCTACATAAAAGATTTGTGGATGTTGTTGCTCATCTTGGGATAAAGAACGCGGTTCCGAAAACGATTATGCAGTTGATGAATGTTGAAGGATTAACAAGAGAAAATGTTGCTAGTCATTTGCAGAAATATCGTCTTTATTTGAAGAGAATGCAAGGGCTTTCAAATGATGCACCTTCTTCTTCTGATCATCTTTTTGCTTCTACACCTGTGCCGCAGAGTTTGCATGAAACCGGTTCTGCTAATCATTCTCATTCACATTCAAATGCTAATCATTCAAATGGAGGGCATGGAAATGGACATGGAAATTCGCATTCTCATTCACATTCGCATCATTCTGTGACGATTCCTATGCCTTATCCACCACCACCTTCGAGTATGATGTCAATGCCGCCTATGATTGGTATGGGACATCATCCACATGGGCATATGGGGATGAGTTTGCCTCCTCCTTCGGGTGGTTCTAGCTCTTATCGCTCACATCCATTTAATATGATGCATCATAGGGATTGGCCTCCGAATCCACATTCTCATCCTCATATGTCTTCCAATGATAGTAATAAATAA
Protein sequence

>MsG0480021035.01.T01

MGEEVRTDEERVTEWEKGLPNLHDLTPLSMALIPPELASAFSISPEPLRTLFDVNRASRNTLSLLRSNSGTITHQINQPVSSEVEDVEEDEEPERDGSGSDSRKHRKIDSVTEEVEAEADSAVRTTETIKRPRLVWTPQLHKRFVDVVAHLGIKNAVPKTIMQLMNVEGLTRENVASHLQKYRLYLKRMQGLSNDAPSSSDHLFASTPVPQSLHETGSANHSHSHSNANHSNGGHGNGHGNSHSHSHSHHSVTIPMPYPPPPSSMMSMPPMIGMGHHPHGHMGMSLPPPSGGSSSYRSHPFNMMHHRDWPPNPHSHPHMSSNDSNK*