AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021648.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021648.01.T01 MTR_4g077970 90.909 495 28 3 1 480 1 493 0.0 895
MsG0480021648.01.T01 MTR_4g077970 96.532 346 9 1 138 480 1 346 0.0 675
MsG0480021648.01.T01 MTR_5g029680 43.287 499 220 18 11 476 31 499 1.72e-104 321
MsG0480021648.01.T01 MTR_3g104550 46.667 225 94 5 11 223 45 255 1.55e-52 182
MsG0480021648.01.T01 MTR_7g095680 48.325 209 91 3 11 207 48 251 5.51e-51 177
MsG0480021648.01.T01 MTR_1g102860 47.642 212 86 6 11 207 39 240 5.95e-48 168
MsG0480021648.01.T01 MTR_1g061170 35.738 305 155 8 10 299 47 325 1.06e-46 168
MsG0480021648.01.T01 MTR_8g105780 36.296 270 150 4 12 268 22 282 1.02e-42 155
MsG0480021648.01.T01 MTR_1g071360 43.721 215 87 5 12 211 32 227 4.46e-41 150
MsG0480021648.01.T01 MTR_1g071360 43.439 221 90 6 7 211 23 224 8.10e-41 150
MsG0480021648.01.T01 MTR_1g061170 35.685 241 128 6 62 299 14 230 6.95e-37 139
MsG0480021648.01.T01 MTR_2g100680 36.715 207 110 5 12 208 31 226 1.96e-34 134
MsG0480021648.01.T01 MTR_8g083100 37.981 208 103 5 13 208 23 216 3.48e-33 131
MsG0480021648.01.T01 MTR_6g043060 35.616 219 115 5 2 208 12 216 1.29e-32 127
MsG0480021648.01.T01 MTR_8g087540 36.898 187 96 4 10 183 10 187 4.98e-31 124
MsG0480021648.01.T01 MTR_2g100670 37.433 187 93 3 12 186 33 207 6.44e-31 122
MsG0480021648.01.T01 MTR_2g100670 37.433 187 93 3 12 186 141 315 3.42e-30 122
MsG0480021648.01.T01 MTR_6g086805 36.916 214 109 8 7 208 6 205 2.21e-29 118
MsG0480021648.01.T01 MTR_2g017890 30.890 191 107 4 10 184 13 194 7.18e-24 103
MsG0480021648.01.T01 MTR_6g044340 29.630 216 128 7 43 257 6 198 9.97e-21 92.0
MsG0480021648.01.T01 MTR_5g010680 46.809 94 36 2 12 93 24 115 4.89e-18 85.9
MsG0480021648.01.T01 MTR_3g101870 33.163 196 68 6 12 146 24 217 5.18e-18 85.9
MsG0480021648.01.T01 MTR_5g017470 43.158 95 40 2 12 94 9 101 1.02e-15 77.8
MsG0480021648.01.T01 MTR_0020s0020 42.553 94 40 2 12 93 33 124 2.56e-15 77.4
MsG0480021648.01.T01 MTR_1g100777 40.426 94 42 2 12 93 18 109 8.46e-15 75.5
MsG0480021648.01.T01 MTR_5g089170 43.158 95 40 2 12 94 25 117 1.91e-14 73.6
MsG0480021648.01.T01 MTR_2g043940 29.927 137 77 2 55 183 15 140 7.51e-14 73.6
MsG0480021648.01.T01 MTR_1g061170 30.909 165 95 4 138 299 1 149 2.81e-13 70.5
MsG0480021648.01.T01 MTR_7g091370 32.903 155 79 4 12 147 22 170 3.20e-13 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021648.01.T01 AT5G16820 44.980 498 199 15 10 476 25 478 3.85e-110 335
MsG0480021648.01.T01 AT5G16820 44.980 498 199 15 10 476 25 478 3.85e-110 335
MsG0480021648.01.T01 AT3G02990 43.469 490 209 10 10 476 21 465 1.72e-105 322
MsG0480021648.01.T01 AT1G32330 40.882 499 221 15 3 476 28 477 3.23e-92 288
MsG0480021648.01.T01 AT1G32330 40.882 499 221 15 3 476 28 477 5.01e-92 288
MsG0480021648.01.T01 AT4G17750 40.079 504 213 19 6 476 46 493 3.33e-86 273
MsG0480021648.01.T01 AT2G26150 50.472 212 83 4 11 208 43 246 5.69e-59 198
MsG0480021648.01.T01 AT2G26150 50.472 212 83 4 11 208 43 246 5.69e-59 198
MsG0480021648.01.T01 AT3G22830 48.095 210 94 3 11 207 60 267 1.16e-51 180
MsG0480021648.01.T01 AT2G26150 52.121 165 71 2 46 208 33 191 8.88e-48 167
MsG0480021648.01.T01 AT2G26150 52.121 165 71 2 46 208 33 191 8.88e-48 167
MsG0480021648.01.T01 AT1G67970 38.462 286 135 10 10 276 17 280 6.38e-42 153
MsG0480021648.01.T01 AT5G03720 36.820 239 120 6 10 233 53 275 2.21e-40 150
MsG0480021648.01.T01 AT5G03720 36.820 239 120 6 10 233 53 275 2.21e-40 150
MsG0480021648.01.T01 AT3G51910 38.356 219 96 5 4 206 21 216 2.70e-37 138
MsG0480021648.01.T01 AT4G13980 34.081 223 120 5 13 222 24 232 2.25e-32 129
MsG0480021648.01.T01 AT5G43840 33.333 243 105 8 11 234 16 220 4.77e-30 119
MsG0480021648.01.T01 AT3G24520 33.645 214 97 6 9 208 14 196 1.97e-29 118
MsG0480021648.01.T01 AT4G18880 30.645 310 178 9 4 298 7 294 1.60e-28 117
MsG0480021648.01.T01 AT5G54070 33.484 221 113 5 9 212 68 271 4.21e-27 111
MsG0480021648.01.T01 AT5G45710 33.921 227 114 7 10 222 11 215 4.04e-26 109
MsG0480021648.01.T01 AT5G45710 33.921 227 114 7 10 222 11 215 4.04e-26 109
MsG0480021648.01.T01 AT3G63350 35.025 197 102 4 12 195 28 211 1.19e-25 106
MsG0480021648.01.T01 AT1G46264 32.161 199 80 5 1 146 23 219 4.70e-20 91.7
MsG0480021648.01.T01 AT4G36990 39.098 133 53 5 1 119 1 119 1.87e-15 77.0
MsG0480021648.01.T01 AT2G41690 40.196 102 47 2 11 100 39 138 2.15e-15 76.3
MsG0480021648.01.T01 AT5G62020 40.625 96 43 2 12 95 23 116 3.08e-14 73.9
MsG0480021648.01.T01 AT4G18870 43.023 86 40 2 13 89 14 99 2.39e-13 70.9
MsG0480021648.01.T01 AT4G18870 37.778 90 42 3 12 89 146 233 3.22e-13 70.5
MsG0480021648.01.T01 AT4G11660 39.362 94 43 2 12 93 59 150 6.30e-13 70.5

Find 112 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGAGGTTCCACCTATTTC+TGG 0.099338 4:+61251602 MsG0480021648.01.T01:CDS
AGTTCATCCACCCAAGTTTC+TGG 0.190167 4:+61251569 MsG0480021648.01.T01:CDS
AGCACACCAAGACAATATTT+TGG 0.232538 4:+61251754 MsG0480021648.01.T01:CDS
CAGGAATCAACGATGAATTC+TGG 0.282845 4:+61251957 MsG0480021648.01.T01:CDS
TTTAGGCCAAGCCCACTTAC+TGG 0.290833 4:+61251989 MsG0480021648.01.T01:CDS
AAATGTAGTTCTCTAGGATA+TGG 0.296292 4:+61252028 MsG0480021648.01.T01:CDS
TGTTGAATCTTCTTTGCTAA+TGG 0.299601 4:-61252073 None:intergenic
GGATGTGATATCAGCTGTTT+TGG 0.314761 4:+61251871 MsG0480021648.01.T01:CDS
TTTGGATGGGACACAGTCTT+TGG 0.329794 4:+61251889 MsG0480021648.01.T01:CDS
AAGAGGATTATGTGGATTCT+TGG 0.333992 4:-61251381 None:intergenic
CAACCGAATCGATCGTTTCA+TGG 0.334490 4:+61250073 MsG0480021648.01.T01:CDS
ATACATGACTGCCCAGAAAT+AGG 0.338814 4:-61251614 None:intergenic
AACTGTTCATTTGCAACATC+TGG 0.343307 4:-61251463 None:intergenic
CTTAAGCAAAACCTACGATA+TGG 0.345914 4:+61250038 MsG0480021648.01.T01:CDS
CTATTGGTCATACAACTTAC+AGG 0.350342 4:-61251656 None:intergenic
TGTGTGGAAGTGGGTAAGTT+TGG 0.353975 4:+61251071 MsG0480021648.01.T01:CDS
CTGAGGTTCCACCTATTTCT+GGG 0.367826 4:+61251603 MsG0480021648.01.T01:CDS
CCGAAGGAACATCATCAATA+AGG 0.369125 4:-61251526 None:intergenic
TCTTTGCTAATGGCAATTCC+TGG 0.373655 4:-61252063 None:intergenic
TTTGCAGCCTCATTAATAGA+AGG 0.376695 4:-61251428 None:intergenic
CATGAATAGTCCGGGCTTCA+TGG 0.377627 4:+61251265 MsG0480021648.01.T01:CDS
CAGAATTCATCGTTGATTCC+TGG 0.379940 4:-61251956 None:intergenic
TTGGTGTGCTGTTGTAAATT+CGG 0.380502 4:-61251741 None:intergenic
AATCCACATAATCCTCTTGA+TGG 0.386726 4:+61251386 MsG0480021648.01.T01:CDS
CATTCCTTCCACGAAACTCT+TGG 0.390343 4:-61250570 None:intergenic
GTGTCTCCAGTAAGTGGGCT+TGG 0.409905 4:-61251995 None:intergenic
CGGTGAGAATGATGATGAAT+TGG 0.410275 4:+61251850 MsG0480021648.01.T01:CDS
CATGAAGCCCGGACTATTCA+TGG 0.411920 4:-61251264 None:intergenic
AACTATCTTCTTCCTGCCCC+TGG 0.417448 4:-61251352 None:intergenic
GATGAACTACTGCTATCTAA+TGG 0.419987 4:-61251554 None:intergenic
ATTTGTTAAAGAATATCAAT+AGG 0.421836 4:+61250979 MsG0480021648.01.T01:CDS
TGAGCGAATTCCCAGCGGTC+GGG 0.422148 4:-61250930 None:intergenic
ATCGTTGATTCCTGGAAGCT+TGG 0.423687 4:-61251948 None:intergenic
AAGAGGTTGAAAGGCTGAAA+AGG 0.431696 4:+61251102 MsG0480021648.01.T01:CDS
CCTTATTGATGATGTTCCTT+CGG 0.432545 4:+61251526 MsG0480021648.01.T01:CDS
GTGTGGAAGTGGGTAAGTTT+GGG 0.436120 4:+61251072 MsG0480021648.01.T01:CDS
AACCGAATCGATCGTTTCAT+GGG 0.436953 4:+61250074 MsG0480021648.01.T01:CDS
GTTGCCCAACATTTACCAAT+TGG 0.441846 4:-61251187 None:intergenic
AATGAAAATTCCAAGCTTCC+AGG 0.444171 4:+61251938 MsG0480021648.01.T01:CDS
GAATGCATCAAGGTTCTTGA+TGG 0.448987 4:-61251504 None:intergenic
GATAACCAATTGGTAAATGT+TGG 0.452224 4:+61251182 MsG0480021648.01.T01:CDS
GAAGATTCAACAAGAAAAGA+TGG 0.457111 4:+61252084 MsG0480021648.01.T01:CDS
CGGAAATCTACTCATGCAAA+TGG 0.457161 4:+61251002 MsG0480021648.01.T01:CDS
AGCAAAGGCCATGAATAGTC+CGG 0.460979 4:+61251256 MsG0480021648.01.T01:CDS
AAAGAGGCGGCTCCAGGGGC+AGG 0.463211 4:+61251340 MsG0480021648.01.T01:CDS
ATAACCAATTGGTAAATGTT+GGG 0.467159 4:+61251183 MsG0480021648.01.T01:CDS
TGGTGTGCTGTTGTAAATTC+GGG 0.470532 4:-61251740 None:intergenic
AAAGTGTTACACCAGAAACT+TGG 0.470921 4:-61251580 None:intergenic
AGGAATCAACGATGAATTCT+GGG 0.476706 4:+61251958 MsG0480021648.01.T01:CDS
TGTTGGGTCATGTGTGGAAG+TGG 0.480175 4:+61251061 MsG0480021648.01.T01:CDS
AGCCGCAGAACCCACCTGTT+GGG 0.484089 4:+61251045 MsG0480021648.01.T01:CDS
GAAAGGTTGACCCCGACCGC+TGG 0.485865 4:+61250919 MsG0480021648.01.T01:CDS
TATTTCTGGGCAGTCATGTA+TGG 0.490052 4:+61251616 MsG0480021648.01.T01:CDS
GACTATTCATGGCCTTTGCT+AGG 0.492617 4:-61251253 None:intergenic
GAGCGAATTCCCAGCGGTCG+GGG 0.492924 4:-61250929 None:intergenic
ACAATCGACTGATAACCAAT+TGG 0.498524 4:+61251172 MsG0480021648.01.T01:CDS
GAAGTTAAATGTAGTTCTCT+AGG 0.498658 4:+61252022 MsG0480021648.01.T01:CDS
ACCCCATGAAACGATCGATT+CGG 0.504242 4:-61250076 None:intergenic
ACTTCCACACATGACCCAAC+AGG 0.505692 4:-61251059 None:intergenic
TCGTCTGTGTCTCCAGTAAG+TGG 0.508093 4:-61252001 None:intergenic
TTCTGGTGTAACACTTTCTG+AGG 0.510063 4:+61251586 MsG0480021648.01.T01:CDS
CAAAATCCAAAATATTGTCT+TGG 0.518046 4:-61251760 None:intergenic
GTACCAGCCTTCTATTAATG+AGG 0.518867 4:+61251421 MsG0480021648.01.T01:CDS
TTGTACCTCAGACATGCTAT+TGG 0.521476 4:-61251672 None:intergenic
GTTCCATCTGTTGAGTAAGA+TGG 0.529932 4:-61252117 None:intergenic
CGCTGGGAATTCGCTCATGA+AGG 0.530003 4:+61250936 MsG0480021648.01.T01:CDS
CAGCCTCATTAATAGAAGGC+TGG 0.531526 4:-61251424 None:intergenic
TAACATTTCCAAGAGTTTCG+TGG 0.532952 4:+61250562 MsG0480021648.01.T01:CDS
AGGATCGAAGATATGACAGC+TGG 0.535692 4:+61249988 None:intergenic
CGTCCATCAAGAGGATTATG+TGG 0.541079 4:-61251389 None:intergenic
CATCAATAAGGAATGCATCA+AGG 0.541216 4:-61251514 None:intergenic
AAAGGTTGACCCCGACCGCT+GGG 0.545860 4:+61250920 MsG0480021648.01.T01:CDS
GAAAATTGAGCCATGAAGCC+CGG 0.546699 4:-61251275 None:intergenic
AAGCCGCAGAACCCACCTGT+TGG 0.557194 4:+61251044 MsG0480021648.01.T01:CDS
TAAGTTTGGGCTTGATGAAG+AGG 0.559114 4:+61251085 MsG0480021648.01.T01:CDS
GCAAAGGCCATGAATAGTCC+GGG 0.565582 4:+61251257 MsG0480021648.01.T01:CDS
AATGAACAGTTCTGCAAGGG+TGG 0.569475 4:+61251475 MsG0480021648.01.T01:CDS
AGAGGTTGAAAGGCTGAAAA+GGG 0.570797 4:+61251103 MsG0480021648.01.T01:CDS
ATGAGCGAATTCCCAGCGGT+CGG 0.572798 4:-61250931 None:intergenic
GATGATGTCGTTCCTAGCAA+AGG 0.573149 4:+61251241 MsG0480021648.01.T01:CDS
ATATGGTTTGACTAAAGACC+AGG 0.574236 4:+61252045 MsG0480021648.01.T01:CDS
GGCTTGATGAAGAGGTTGAA+AGG 0.575265 4:+61251093 MsG0480021648.01.T01:CDS
GGACCATCTTACTCAACAGA+TGG 0.577724 4:+61252114 MsG0480021648.01.T01:CDS
AAGTGTTACACCAGAAACTT+GGG 0.578836 4:-61251579 None:intergenic
ATTTCCAAGAGTTTCGTGGA+AGG 0.584043 4:+61250566 MsG0480021648.01.T01:CDS
ACAGATGGAACTTCTTGCAT+CGG 0.591151 4:+61252129 MsG0480021648.01.T01:CDS
TCCACACATGACCCAACAGG+TGG 0.591825 4:-61251056 None:intergenic
AGGATTATGTGGATTCTTGG+TGG 0.592009 4:-61251378 None:intergenic
GACCCAACAGGTGGGTTCTG+CGG 0.593008 4:-61251047 None:intergenic
AGTAATAGACATGTTACCGC+TGG 0.593286 4:+61251314 MsG0480021648.01.T01:CDS
CGTCTGTGTCTCCAGTAAGT+GGG 0.594211 4:-61252000 None:intergenic
TGTAAATTCGGGAAATTCAG+CGG 0.604212 4:-61251729 None:intergenic
ACAGAGTCAGTCAACACAGC+AGG 0.605291 4:-61251701 None:intergenic
TGCATCAAGGTTCTTGATGG+AGG 0.607948 4:-61251501 None:intergenic
AGAGTCATCAACCATATCGT+AGG 0.608336 4:-61250049 None:intergenic
ACAAGAAAAGATGGAGCACA+TGG 0.615775 4:+61252093 MsG0480021648.01.T01:CDS
TGGGCAACGTGTGCAAGTGA+TGG 0.618938 4:+61251202 MsG0480021648.01.T01:CDS
ACTACTGCTATCTAATGGAA+TGG 0.622288 4:-61251549 None:intergenic
CTATCTAATGGAATGGCCGA+AGG 0.622401 4:-61251542 None:intergenic
TTAACAACACGTCCATCAAG+AGG 0.623081 4:-61251398 None:intergenic
CCCACCTGTTGGGTCATGTG+TGG 0.640259 4:+61251055 MsG0480021648.01.T01:CDS
TGTTAAAGAATATCAATAGG+CGG 0.647648 4:+61250982 MsG0480021648.01.T01:CDS
TATGACCAATAGCATGTCTG+AGG 0.654015 4:+61251667 MsG0480021648.01.T01:CDS
TGCAAATGAACAGTTCTGCA+AGG 0.655638 4:+61251471 MsG0480021648.01.T01:CDS
CATGACTGCCCAGAAATAGG+TGG 0.662832 4:-61251611 None:intergenic
GTTGGGTCATGTGTGGAAGT+GGG 0.670827 4:+61251062 MsG0480021648.01.T01:CDS
ACCGAATCGATCGTTTCATG+GGG 0.677166 4:+61250075 MsG0480021648.01.T01:CDS
GCAAATGAACAGTTCTGCAA+GGG 0.681864 4:+61251472 MsG0480021648.01.T01:CDS
CCACACATGACCCAACAGGT+GGG 0.694523 4:-61251055 None:intergenic
TGTTACACCAGAAACTTGGG+TGG 0.706092 4:-61251576 None:intergenic
TGTTAGAACTGAATTCTACA+CGG 0.709397 4:+61250625 MsG0480021648.01.T01:CDS
CTTCATGAGCGAATTCCCAG+CGG 0.750748 4:-61250935 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTCTTAGAAAATATTCAT+CGG - Chr4:61250533-61250552 None:intergenic 15.0%
!!! ATTTGTTAAAGAATATCAAT+AGG + Chr4:61250979-61250998 MsG0480021648.01.T01:CDS 15.0%
!! ATAGTATTAAAAAAGCAAGA+AGG - Chr4:61250796-61250815 None:intergenic 20.0%
!! TACTATACATTTAATAATGC+TGG + Chr4:61250810-61250829 MsG0480021648.01.T01:intron 20.0%
!!! GATGAATATTTTCTAAGAAT+TGG + Chr4:61250532-61250551 MsG0480021648.01.T01:intron 20.0%
!!! TTAAGTAGTAATAGGATTTT+AGG + Chr4:61250669-61250688 MsG0480021648.01.T01:intron 20.0%
!!! TTGTTGTGTTTGAAATATTT+AGG - Chr4:61250159-61250178 None:intergenic 20.0%
!!! TTTTCACCTTTTCTTAATTA+GGG + Chr4:61250231-61250250 MsG0480021648.01.T01:intron 20.0%
!!! TTTTTCACCTTTTCTTAATT+AGG + Chr4:61250230-61250249 MsG0480021648.01.T01:intron 20.0%
! ATAACCAATTGGTAAATGTT+GGG + Chr4:61251183-61251202 MsG0480021648.01.T01:CDS 25.0%
! CAAAATCCAAAATATTGTCT+TGG - Chr4:61251763-61251782 None:intergenic 25.0%
! GTAAAACCCTAATTAAGAAA+AGG - Chr4:61250240-61250259 None:intergenic 25.0%
! GTAATTAGAGAAGTTACATT+CGG + Chr4:61250417-61250436 MsG0480021648.01.T01:intron 25.0%
! GTTCAGATCATATTAACTTA+TGG + Chr4:61250273-61250292 MsG0480021648.01.T01:intron 25.0%
! TAATTAGAGAAGTTACATTC+GGG + Chr4:61250418-61250437 MsG0480021648.01.T01:intron 25.0%
! TGTTAAAGAATATCAATAGG+CGG + Chr4:61250982-61251001 MsG0480021648.01.T01:CDS 25.0%
!! AATCCTGATCAAAATTTTGT+GGG + Chr4:61251821-61251840 MsG0480021648.01.T01:CDS 25.0%
!! CTTAATTGTAATTTGGTGAT+TGG + Chr4:61250378-61250397 MsG0480021648.01.T01:intron 25.0%
!! TTATGGACGAAGATTTTATT+AGG + Chr4:61250290-61250309 MsG0480021648.01.T01:intron 25.0%
!! TTGTATACTTTTAATCATGC+TGG + Chr4:61250845-61250864 MsG0480021648.01.T01:intron 25.0%
!!! AATTCTGGGAACAATTTTTT+AGG + Chr4:61251972-61251991 MsG0480021648.01.T01:CDS 25.0%
!!! AGTAATAGGATTTTAGGTTA+TGG + Chr4:61250675-61250694 MsG0480021648.01.T01:intron 25.0%
!!! ATCTTTTTTATTCACAAGCA+GGG + Chr4:61250891-61250910 MsG0480021648.01.T01:intron 25.0%
!!! ATTTTCTAAGAATTGGATGT+AGG + Chr4:61250539-61250558 MsG0480021648.01.T01:intron 25.0%
!!! CAAGACAATATTTTGGATTT+TGG + Chr4:61251761-61251780 MsG0480021648.01.T01:CDS 25.0%
!!! TATCTTTTTTATTCACAAGC+AGG + Chr4:61250890-61250909 MsG0480021648.01.T01:intron 25.0%
AGTAAAGTAAGAACATACGT+AGG - Chr4:61250211-61250230 None:intergenic 30.0%
ATATTTAGGCAAGATGTGTT+TGG - Chr4:61250145-61250164 None:intergenic 30.0%
ATTAAAAAAGCAAGAAGGAG+AGG - Chr4:61250791-61250810 None:intergenic 30.0%
GAAGATTCAACAAGAAAAGA+TGG + Chr4:61252084-61252103 MsG0480021648.01.T01:CDS 30.0%
GAAGTTAAATGTAGTTCTCT+AGG + Chr4:61252022-61252041 MsG0480021648.01.T01:CDS 30.0%
GACTGCAATTAAGTAGTAAT+AGG + Chr4:61250661-61250680 MsG0480021648.01.T01:intron 30.0%
GATAACCAATTGGTAAATGT+TGG + Chr4:61251182-61251201 MsG0480021648.01.T01:CDS 30.0%
GTTAAGATAGTTACCCTATT+GGG + Chr4:61250697-61250716 MsG0480021648.01.T01:intron 30.0%
TAACTAATGAATCCCCAATA+GGG - Chr4:61250713-61250732 None:intergenic 30.0%
TGTTAGAACTGAATTCTACA+CGG + Chr4:61250625-61250644 MsG0480021648.01.T01:CDS 30.0%
TGTTGAATCTTCTTTGCTAA+TGG - Chr4:61252076-61252095 None:intergenic 30.0%
TTAAGATAGTTACCCTATTG+GGG + Chr4:61250698-61250717 MsG0480021648.01.T01:intron 30.0%
! ATCCTGATCAAAATTTTGTG+GGG + Chr4:61251822-61251841 MsG0480021648.01.T01:CDS 30.0%
! GAATCCTGATCAAAATTTTG+TGG + Chr4:61251820-61251839 MsG0480021648.01.T01:CDS 30.0%
!! AAATGTAGTTCTCTAGGATA+TGG + Chr4:61252028-61252047 MsG0480021648.01.T01:CDS 30.0%
AACTGTTCATTTGCAACATC+TGG - Chr4:61251466-61251485 None:intergenic 35.0%
AAGAGGATTATGTGGATTCT+TGG - Chr4:61251384-61251403 None:intergenic 35.0%
AAGTGTTACACCAGAAACTT+GGG - Chr4:61251582-61251601 None:intergenic 35.0%
AATCCACATAATCCTCTTGA+TGG + Chr4:61251386-61251405 MsG0480021648.01.T01:CDS 35.0%
AATGAAAATTCCAAGCTTCC+AGG + Chr4:61251938-61251957 MsG0480021648.01.T01:CDS 35.0%
ACAATCGACTGATAACCAAT+TGG + Chr4:61251172-61251191 MsG0480021648.01.T01:CDS 35.0%
ACTACTGCTATCTAATGGAA+TGG - Chr4:61251552-61251571 None:intergenic 35.0%
AGGAATCAACGATGAATTCT+GGG + Chr4:61251958-61251977 MsG0480021648.01.T01:CDS 35.0%
ATATGGTTTGACTAAAGACC+AGG + Chr4:61252045-61252064 MsG0480021648.01.T01:CDS 35.0%
CAAGATGTGTTTGGAGAAAT+CGG - Chr4:61250136-61250155 None:intergenic 35.0%
CATCAATAAGGAATGCATCA+AGG - Chr4:61251517-61251536 None:intergenic 35.0%
CATTTGCAAACTGCAAAAAG+TGG - Chr4:61250607-61250626 None:intergenic 35.0%
CTAACTAATGAATCCCCAAT+AGG - Chr4:61250714-61250733 None:intergenic 35.0%
CTATTGGTCATACAACTTAC+AGG - Chr4:61251659-61251678 None:intergenic 35.0%
CTTAAGCAAAACCTACGATA+TGG + Chr4:61250038-61250057 MsG0480021648.01.T01:CDS 35.0%
GAACGCGCTTAATTGTAATT+TGG + Chr4:61250371-61250390 MsG0480021648.01.T01:intron 35.0%
GATGAACTACTGCTATCTAA+TGG - Chr4:61251557-61251576 None:intergenic 35.0%
GGTTAAGATAGTTACCCTAT+TGG + Chr4:61250696-61250715 MsG0480021648.01.T01:intron 35.0%
TAACATTTCCAAGAGTTTCG+TGG + Chr4:61250562-61250581 MsG0480021648.01.T01:CDS 35.0%
TGTAAATTCGGGAAATTCAG+CGG - Chr4:61251732-61251751 None:intergenic 35.0%
TTTGCAGCCTCATTAATAGA+AGG - Chr4:61251431-61251450 None:intergenic 35.0%
! AAAGTGTTACACCAGAAACT+TGG - Chr4:61251583-61251602 None:intergenic 35.0%
! AGCACACCAAGACAATATTT+TGG + Chr4:61251754-61251773 MsG0480021648.01.T01:CDS 35.0%
! CGCTCATGAAGGATTTTTAA+GGG + Chr4:61250947-61250966 MsG0480021648.01.T01:CDS 35.0%
! TAGGTTTTGCTTAAGAATGG+TGG - Chr4:61250033-61250052 None:intergenic 35.0%
! TCGCTCATGAAGGATTTTTA+AGG + Chr4:61250946-61250965 MsG0480021648.01.T01:CDS 35.0%
! TCGTAGGTTTTGCTTAAGAA+TGG - Chr4:61250036-61250055 None:intergenic 35.0%
! TGTAATTTGGTGATTGGAGA+GGG + Chr4:61250384-61250403 MsG0480021648.01.T01:intron 35.0%
! TTGGTGTGCTGTTGTAAATT+CGG - Chr4:61251744-61251763 None:intergenic 35.0%
! TTGTAATTTGGTGATTGGAG+AGG + Chr4:61250383-61250402 MsG0480021648.01.T01:intron 35.0%
!! CCTTATTGATGATGTTCCTT+CGG + Chr4:61251526-61251545 MsG0480021648.01.T01:CDS 35.0%
!! GCTCATGAAGGATTTTTAAG+GGG + Chr4:61250948-61250967 MsG0480021648.01.T01:CDS 35.0%
!! TGATATCAGCTGTTTTGGAT+GGG + Chr4:61251876-61251895 MsG0480021648.01.T01:CDS 35.0%
!! TTGGATTTTGGTGAAGTTCA+AGG + Chr4:61251773-61251792 MsG0480021648.01.T01:CDS 35.0%
AACCGAATCGATCGTTTCAT+GGG + Chr4:61250074-61250093 MsG0480021648.01.T01:CDS 40.0%
AAGAGGTTGAAAGGCTGAAA+AGG + Chr4:61251102-61251121 MsG0480021648.01.T01:CDS 40.0%
ACAAGAAAAGATGGAGCACA+TGG + Chr4:61252093-61252112 MsG0480021648.01.T01:CDS 40.0%
ACAGATGGAACTTCTTGCAT+CGG + Chr4:61252129-61252148 MsG0480021648.01.T01:CDS 40.0%
ACAGTAACAGTAACACTAGC+AGG - Chr4:61250483-61250502 None:intergenic 40.0%
AGAGGTTGAAAGGCTGAAAA+GGG + Chr4:61251103-61251122 MsG0480021648.01.T01:CDS 40.0%
AGAGTCATCAACCATATCGT+AGG - Chr4:61250052-61250071 None:intergenic 40.0%
AGGATTATGTGGATTCTTGG+TGG - Chr4:61251381-61251400 None:intergenic 40.0%
AGTAATAGACATGTTACCGC+TGG + Chr4:61251314-61251333 MsG0480021648.01.T01:CDS 40.0%
ATACATGACTGCCCAGAAAT+AGG - Chr4:61251617-61251636 None:intergenic 40.0%
ATGTTACCGCTGGTAAAAAG+AGG + Chr4:61251324-61251343 MsG0480021648.01.T01:CDS 40.0%
ATTTCCAAGAGTTTCGTGGA+AGG + Chr4:61250566-61250585 MsG0480021648.01.T01:CDS 40.0%
CAAAGTCAAGATACTTGTCC+TGG + Chr4:61250746-61250765 MsG0480021648.01.T01:intron 40.0%
CAGGAATCAACGATGAATTC+TGG + Chr4:61251957-61251976 MsG0480021648.01.T01:CDS 40.0%
CCGAAGGAACATCATCAATA+AGG - Chr4:61251529-61251548 None:intergenic 40.0%
CGGAAATCTACTCATGCAAA+TGG + Chr4:61251002-61251021 MsG0480021648.01.T01:CDS 40.0%
GAATGCATCAAGGTTCTTGA+TGG - Chr4:61251507-61251526 None:intergenic 40.0%
GCAAATGAACAGTTCTGCAA+GGG + Chr4:61251472-61251491 MsG0480021648.01.T01:CDS 40.0%
GTACCAGCCTTCTATTAATG+AGG + Chr4:61251421-61251440 MsG0480021648.01.T01:CDS 40.0%
GTTACATTCGGGATGCATTT+CGG + Chr4:61250429-61250448 MsG0480021648.01.T01:intron 40.0%
GTTCCATCTGTTGAGTAAGA+TGG - Chr4:61252120-61252139 None:intergenic 40.0%
GTTGCCCAACATTTACCAAT+TGG - Chr4:61251190-61251209 None:intergenic 40.0%
TAAGTTTGGGCTTGATGAAG+AGG + Chr4:61251085-61251104 MsG0480021648.01.T01:CDS 40.0%
TATGACCAATAGCATGTCTG+AGG + Chr4:61251667-61251686 MsG0480021648.01.T01:CDS 40.0%
TCTTTGCTAATGGCAATTCC+TGG - Chr4:61252066-61252085 None:intergenic 40.0%
TGCAAATGAACAGTTCTGCA+AGG + Chr4:61251471-61251490 MsG0480021648.01.T01:CDS 40.0%
TTAACAACACGTCCATCAAG+AGG - Chr4:61251401-61251420 None:intergenic 40.0%
TTCTGGTGTAACACTTTCTG+AGG + Chr4:61251586-61251605 MsG0480021648.01.T01:CDS 40.0%
TTGTACCTCAGACATGCTAT+TGG - Chr4:61251675-61251694 None:intergenic 40.0%
! CAAAATTTTGTGGGGTCTGT+CGG + Chr4:61251830-61251849 MsG0480021648.01.T01:CDS 40.0%
! CGGTGAGAATGATGATGAAT+TGG + Chr4:61251850-61251869 MsG0480021648.01.T01:CDS 40.0%
! GGATGTGATATCAGCTGTTT+TGG + Chr4:61251871-61251890 MsG0480021648.01.T01:CDS 40.0%
! TGCTAGTGTTACTGTTACTG+TGG + Chr4:61250482-61250501 MsG0480021648.01.T01:intron 40.0%
! TGGTGTGCTGTTGTAAATTC+GGG - Chr4:61251743-61251762 None:intergenic 40.0%
! TTTTGGTGAAGTTCAAGGAC+TGG + Chr4:61251778-61251797 MsG0480021648.01.T01:CDS 40.0%
!! CAGAATTCATCGTTGATTCC+TGG - Chr4:61251959-61251978 None:intergenic 40.0%
!! GACCCCACAAAATTTTGATC+AGG - Chr4:61251827-61251846 None:intergenic 40.0%
!! GTGATATCAGCTGTTTTGGA+TGG + Chr4:61251875-61251894 MsG0480021648.01.T01:CDS 40.0%
!! TATTTCTGGGCAGTCATGTA+TGG + Chr4:61251616-61251635 MsG0480021648.01.T01:CDS 40.0%
!!! TCACAAGCAGGGTTTTAGAA+AGG + Chr4:61250902-61250921 MsG0480021648.01.T01:intron 40.0%
AATGAACAGTTCTGCAAGGG+TGG + Chr4:61251475-61251494 MsG0480021648.01.T01:CDS 45.0%
ACCCCATGAAACGATCGATT+CGG - Chr4:61250079-61250098 None:intergenic 45.0%
ACCGAATCGATCGTTTCATG+GGG + Chr4:61250075-61250094 MsG0480021648.01.T01:CDS 45.0%
AGCAAAGGCCATGAATAGTC+CGG + Chr4:61251256-61251275 MsG0480021648.01.T01:CDS 45.0%
AGTTCATCCACCCAAGTTTC+TGG + Chr4:61251569-61251588 MsG0480021648.01.T01:CDS 45.0%
ATATTCATCGGTACACCGAG+CGG - Chr4:61250521-61250540 None:intergenic 45.0%
CAACCGAATCGATCGTTTCA+TGG + Chr4:61250073-61250092 MsG0480021648.01.T01:CDS 45.0%
CAGCCTCATTAATAGAAGGC+TGG - Chr4:61251427-61251446 None:intergenic 45.0%
CATTCCTTCCACGAAACTCT+TGG - Chr4:61250573-61250592 None:intergenic 45.0%
CGTCCATCAAGAGGATTATG+TGG - Chr4:61251392-61251411 None:intergenic 45.0%
CTATCTAATGGAATGGCCGA+AGG - Chr4:61251545-61251564 None:intergenic 45.0%
GAAAATTGAGCCATGAAGCC+CGG - Chr4:61251278-61251297 None:intergenic 45.0%
GACTATTCATGGCCTTTGCT+AGG - Chr4:61251256-61251275 None:intergenic 45.0%
GATGATGTCGTTCCTAGCAA+AGG + Chr4:61251241-61251260 MsG0480021648.01.T01:CDS 45.0%
GATGTGTTTGGAGAAATCGG+TGG - Chr4:61250133-61250152 None:intergenic 45.0%
GGACCATCTTACTCAACAGA+TGG + Chr4:61252114-61252133 MsG0480021648.01.T01:CDS 45.0%
GGCTTGATGAAGAGGTTGAA+AGG + Chr4:61251093-61251112 MsG0480021648.01.T01:CDS 45.0%
GGTGGAATTGAAAACGACGA+AGG - Chr4:61250115-61250134 None:intergenic 45.0%
GTGTGGAAGTGGGTAAGTTT+GGG + Chr4:61251072-61251091 MsG0480021648.01.T01:CDS 45.0%
GTTCAATTGTCTGACGAAGC+TGG - Chr4:61250187-61250206 None:intergenic 45.0%
TGCAAACTGCAAAAAGTGGC+TGG - Chr4:61250603-61250622 None:intergenic 45.0%
TGCATCAAGGTTCTTGATGG+AGG - Chr4:61251504-61251523 None:intergenic 45.0%
TGTGTGGAAGTGGGTAAGTT+TGG + Chr4:61251071-61251090 MsG0480021648.01.T01:CDS 45.0%
TGTTACACCAGAAACTTGGG+TGG - Chr4:61251579-61251598 None:intergenic 45.0%
TTACCGCTGGTAAAAAGAGG+CGG + Chr4:61251327-61251346 MsG0480021648.01.T01:CDS 45.0%
TTTGGATGGGACACAGTCTT+TGG + Chr4:61251889-61251908 MsG0480021648.01.T01:CDS 45.0%
! CTGAGGTTCCACCTATTTCT+GGG + Chr4:61251603-61251622 MsG0480021648.01.T01:CDS 45.0%
! TCTGAGGTTCCACCTATTTC+TGG + Chr4:61251602-61251621 MsG0480021648.01.T01:CDS 45.0%
!! ATCGTTGATTCCTGGAAGCT+TGG - Chr4:61251951-61251970 None:intergenic 45.0%
AACTATCTTCTTCCTGCCCC+TGG - Chr4:61251355-61251374 None:intergenic 50.0%
ACAGAGTCAGTCAACACAGC+AGG - Chr4:61251704-61251723 None:intergenic 50.0%
ACTTCCACACATGACCCAAC+AGG - Chr4:61251062-61251081 None:intergenic 50.0%
AGATACTTGTCCTGGTGCTC+TGG + Chr4:61250754-61250773 MsG0480021648.01.T01:intron 50.0%
CATGAAGCCCGGACTATTCA+TGG - Chr4:61251267-61251286 None:intergenic 50.0%
CATGAATAGTCCGGGCTTCA+TGG + Chr4:61251265-61251284 MsG0480021648.01.T01:CDS 50.0%
CATGACTGCCCAGAAATAGG+TGG - Chr4:61251614-61251633 None:intergenic 50.0%
CGTCTGTGTCTCCAGTAAGT+GGG - Chr4:61252003-61252022 None:intergenic 50.0%
CTTCATGAGCGAATTCCCAG+CGG - Chr4:61250938-61250957 None:intergenic 50.0%
GCAAAGGCCATGAATAGTCC+GGG + Chr4:61251257-61251276 MsG0480021648.01.T01:CDS 50.0%
GTTGGGTCATGTGTGGAAGT+GGG + Chr4:61251062-61251081 MsG0480021648.01.T01:CDS 50.0%
TCGTCTGTGTCTCCAGTAAG+TGG - Chr4:61252004-61252023 None:intergenic 50.0%
TGTTGGGTCATGTGTGGAAG+TGG + Chr4:61251061-61251080 MsG0480021648.01.T01:CDS 50.0%
TTTAGGCCAAGCCCACTTAC+TGG + Chr4:61251989-61252008 MsG0480021648.01.T01:CDS 50.0%
! AAATTCAGCACCAGAGCACC+AGG - Chr4:61250767-61250786 None:intergenic 50.0%
ATGAGCGAATTCCCAGCGGT+CGG - Chr4:61250934-61250953 None:intergenic 55.0%
CCACACATGACCCAACAGGT+GGG - Chr4:61251058-61251077 None:intergenic 55.0%
CGCTGGGAATTCGCTCATGA+AGG + Chr4:61250936-61250955 MsG0480021648.01.T01:CDS 55.0%
GGTAAAAAGAGGCGGCTCCA+GGG + Chr4:61251335-61251354 MsG0480021648.01.T01:CDS 55.0%
GTAAAAAGAGGCGGCTCCAG+GGG + Chr4:61251336-61251355 MsG0480021648.01.T01:CDS 55.0%
TCCACACATGACCCAACAGG+TGG - Chr4:61251059-61251078 None:intergenic 55.0%
TGGGCAACGTGTGCAAGTGA+TGG + Chr4:61251202-61251221 MsG0480021648.01.T01:CDS 55.0%
TGGTAAAAAGAGGCGGCTCC+AGG + Chr4:61251334-61251353 MsG0480021648.01.T01:CDS 55.0%
! GAGCCGCCTCTTTTTACCAG+CGG - Chr4:61251333-61251352 None:intergenic 55.0%
! GTGTCTCCAGTAAGTGGGCT+TGG - Chr4:61251998-61252017 None:intergenic 55.0%
AAAGGTTGACCCCGACCGCT+GGG + Chr4:61250920-61250939 MsG0480021648.01.T01:CDS 60.0%
AAGCCGCAGAACCCACCTGT+TGG + Chr4:61251044-61251063 MsG0480021648.01.T01:CDS 60.0%
AGCCGCAGAACCCACCTGTT+GGG + Chr4:61251045-61251064 MsG0480021648.01.T01:CDS 60.0%
CCCACCTGTTGGGTCATGTG+TGG + Chr4:61251055-61251074 MsG0480021648.01.T01:CDS 60.0%
GGTGATTGGAGAGGGAGCTG+TGG + Chr4:61250392-61250411 MsG0480021648.01.T01:intron 60.0%
TGAGCGAATTCCCAGCGGTC+GGG - Chr4:61250933-61250952 None:intergenic 60.0%
! GACCCAACAGGTGGGTTCTG+CGG - Chr4:61251050-61251069 None:intergenic 60.0%
CGGTACACCGAGCGGAAGCA+TGG - Chr4:61250513-61250532 None:intergenic 65.0%
GAAAGGTTGACCCCGACCGC+TGG + Chr4:61250919-61250938 MsG0480021648.01.T01:CDS 65.0%
GAGCGAATTCCCAGCGGTCG+GGG - Chr4:61250932-61250951 None:intergenic 65.0%
! GGTGCTGCCATGCTTCCGCT+CGG + Chr4:61250503-61250522 MsG0480021648.01.T01:intron 65.0%
AAAGAGGCGGCTCCAGGGGC+AGG + Chr4:61251340-61251359 MsG0480021648.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 61250000 61252171 61250000 ID=MsG0480021648.01;Name=MsG0480021648.01
Chr4 mRNA 61250000 61252171 61250000 ID=MsG0480021648.01.T01;Parent=MsG0480021648.01;Name=MsG0480021648.01.T01;_AED=0.30;_eAED=0.30;_QI=0|0|0|1|1|1|3|0|480
Chr4 exon 61250000 61250096 61250000 ID=MsG0480021648.01.T01:exon:9580;Parent=MsG0480021648.01.T01
Chr4 exon 61250561 61250646 61250561 ID=MsG0480021648.01.T01:exon:9579;Parent=MsG0480021648.01.T01
Chr4 exon 61250912 61252171 61250912 ID=MsG0480021648.01.T01:exon:9578;Parent=MsG0480021648.01.T01
Chr4 CDS 61250000 61250096 61250000 ID=MsG0480021648.01.T01:cds;Parent=MsG0480021648.01.T01
Chr4 CDS 61250561 61250646 61250561 ID=MsG0480021648.01.T01:cds;Parent=MsG0480021648.01.T01
Chr4 CDS 61250912 61252171 61250912 ID=MsG0480021648.01.T01:cds;Parent=MsG0480021648.01.T01
Gene Sequence

>MsG0480021648.01.T01

ATGACAGCTGGAATCGCGAATTATGTTTTGCCACCATTCTTAAGCAAAACCTACGATATGGTTGATGACTCTTCAACCGAATCGATCGTTTCATGGGGTAACATTTCCAAGAGTTTCGTGGAAGGAATGTTTCAAACCAGCCACTTTTTGCAGTTTGCAAATGTTAGAACTGAATTCTACACGGGTTTTAGAAAGGTTGACCCCGACCGCTGGGAATTCGCTCATGAAGGATTTTTAAGGGGTCAAAAACATTTGTTAAAGAATATCAATAGGCGGAAATCTACTCATGCAAATGGTAATAATCAGCAGCTATCTAAGCCGCAGAACCCACCTGTTGGGTCATGTGTGGAAGTGGGTAAGTTTGGGCTTGATGAAGAGGTTGAAAGGCTGAAAAGGGACAAGAATGTTCTTATGCAAGAACTTGTTAAGTTAAGACAGCAACAACAATCGACTGATAACCAATTGGTAAATGTTGGGCAACGTGTGCAAGTGATGGAGCAGCGTCAGCAACAGATGATGTCGTTCCTAGCAAAGGCCATGAATAGTCCGGGCTTCATGGCTCAATTTTCACAGCAGCAGAATGAAAGTAATAGACATGTTACCGCTGGTAAAAAGAGGCGGCTCCAGGGGCAGGAAGAAGATAGTTTAGCCACCAAGAATCCACATAATCCTCTTGATGGACGTGTTGTTAAGTACCAGCCTTCTATTAATGAGGCTGCAAAAACATTATTTAACCAGATGTTGCAAATGAACAGTTCTGCAAGGGTGGATTCCTCCATCAAGAACCTTGATGCATTCCTTATTGATGATGTTCCTTCGGCCATTCCATTAGATAGCAGTAGTTCATCCACCCAAGTTTCTGGTGTAACACTTTCTGAGGTTCCACCTATTTCTGGGCAGTCATGTATGGCAGTAGAATCTCAATTTCCTGTAAGTTGTATGACCAATAGCATGTCTGAGGTACAATCTTCACCTGCTGTGTTGACTGACTCTGTCAAAACCGCTGAATTTCCCGAATTTACAACAGCACACCAAGACAATATTTTGGATTTTGGTGAAGTTCAAGGACTGGCTACAGAAAGCAGCTTCATGAATCCTGATCAAAATTTTGTGGGGTCTGTCGGTGAGAATGATGATGAATTGGATGTGATATCAGCTGTTTTGGATGGGACACAGTCTTTGGAAGCTGATGCTTTTTCTTCTGATGCGAATGAAAATTCCAAGCTTCCAGGAATCAACGATGAATTCTGGGAACAATTTTTTAGGCCAAGCCCACTTACTGGAGACACAGACGAAGTTAAATGTAGTTCTCTAGGATATGGTTTGACTAAAGACCAGGAATTGCCATTAGCAAAGAAGATTCAACAAGAAAAGATGGAGCACATGGACCATCTTACTCAACAGATGGAACTTCTTGCATCGGACTCAACATTGTGTATATAA
Protein sequence

>MsG0480021648.01.T01

MTAGIANYVLPPFLSKTYDMVDDSSTESIVSWGNISKSFVEGMFQTSHFLQFANVRTEFYTGFRKVDPDRWEFAHEGFLRGQKHLLKNINRRKSTHANGNNQQLSKPQNPPVGSCVEVGKFGLDEEVERLKRDKNVLMQELVKLRQQQQSTDNQLVNVGQRVQVMEQRQQQMMSFLAKAMNSPGFMAQFSQQQNESNRHVTAGKKRRLQGQEEDSLATKNPHNPLDGRVVKYQPSINEAAKTLFNQMLQMNSSARVDSSIKNLDAFLIDDVPSAIPLDSSSSSTQVSGVTLSEVPPISGQSCMAVESQFPVSCMTNSMSEVQSSPAVLTDSVKTAEFPEFTTAHQDNILDFGEVQGLATESSFMNPDQNFVGSVGENDDELDVISAVLDGTQSLEADAFSSDANENSKLPGINDEFWEQFFRPSPLTGDTDEVKCSSLGYGLTKDQELPLAKKIQQEKMEHMDHLTQQMELLASDSTLCI*