AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021850.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021850.01.T01 MTR_4g077760 97.778 315 7 0 11 325 189 503 0.0 627
MsG0480021850.01.T01 MTR_3g056110 35.410 305 183 8 35 328 195 496 2.46e-48 170
MsG0480021850.01.T01 MTR_2g082090 37.748 302 166 10 41 327 238 532 7.53e-46 164
MsG0480021850.01.T01 MTR_5g094450 33.981 309 186 7 35 328 181 486 7.77e-46 163
MsG0480021850.01.T01 MTR_3g065980 35.522 335 181 12 12 325 169 489 8.21e-46 163
MsG0480021850.01.T01 MTR_3g089055 32.087 321 206 5 16 326 199 517 1.57e-45 163
MsG0480021850.01.T01 MTR_1g029420 32.500 320 204 5 16 325 226 543 1.96e-45 163
MsG0480021850.01.T01 MTR_3g089055 32.087 321 206 5 16 326 204 522 2.02e-45 162
MsG0480021850.01.T01 MTR_5g097480 33.433 335 211 7 4 328 166 498 2.56e-45 162
MsG0480021850.01.T01 MTR_7g074650 32.698 315 192 8 18 325 441 742 2.91e-41 153
MsG0480021850.01.T01 MTR_4g133660 34.219 301 184 8 38 327 199 496 7.82e-41 150
MsG0480021850.01.T01 MTR_7g027190 31.875 320 181 7 31 325 316 623 8.26e-41 151
MsG0480021850.01.T01 MTR_1g069725 33.670 297 181 7 12 306 106 388 6.71e-40 146
MsG0480021850.01.T01 MTR_3g072710 30.938 320 199 6 25 325 132 448 1.37e-39 146
MsG0480021850.01.T01 MTR_1g096030 32.229 332 197 10 11 325 76 396 3.53e-37 139
MsG0480021850.01.T01 MTR_2g097410 29.712 313 198 8 31 327 387 693 1.67e-36 139
MsG0480021850.01.T01 MTR_4g104020 27.327 333 216 5 12 325 146 471 1.77e-36 137
MsG0480021850.01.T01 MTR_2g026250 31.229 301 189 8 38 325 252 547 5.44e-36 137
MsG0480021850.01.T01 MTR_4g076020 31.494 308 171 11 29 325 101 379 1.24e-35 134
MsG0480021850.01.T01 MTR_4g102790 29.483 329 208 11 12 325 289 608 3.07e-35 135
MsG0480021850.01.T01 MTR_4g064150 29.524 315 198 8 31 327 377 685 4.54e-35 135
MsG0480021850.01.T01 MTR_4g064200 28.750 320 208 7 12 318 281 593 3.38e-34 132
MsG0480021850.01.T01 MTR_4g064180 30.195 308 195 8 38 327 273 578 5.63e-34 132
MsG0480021850.01.T01 MTR_4g097080 28.615 325 213 7 16 326 134 453 7.75e-34 130
MsG0480021850.01.T01 MTR_4g064120 28.939 311 205 7 31 327 270 578 1.23e-33 130
MsG0480021850.01.T01 MTR_5g058860 32.721 272 173 4 43 304 150 421 8.36e-33 127
MsG0480021850.01.T01 MTR_4g064160 29.091 330 214 7 12 327 313 636 1.53e-32 128
MsG0480021850.01.T01 MTR_2g097463 28.267 329 215 7 12 327 287 607 2.06e-32 127
MsG0480021850.01.T01 MTR_2g097310 28.616 318 204 9 13 317 269 576 4.45e-32 126
MsG0480021850.01.T01 MTR_7g062120 28.391 317 206 7 30 327 378 692 7.66e-32 126
MsG0480021850.01.T01 MTR_2g097380 27.946 297 182 8 31 317 240 514 1.71e-31 124
MsG0480021850.01.T01 MTR_2g097467 26.829 328 221 6 12 327 286 606 1.93e-31 124
MsG0480021850.01.T01 MTR_2g034250 28.571 308 201 7 12 305 207 509 7.22e-31 122
MsG0480021850.01.T01 MTR_2g034280 27.576 330 218 8 12 325 200 524 1.10e-30 122
MsG0480021850.01.T01 MTR_2g097350 28.750 320 202 9 13 316 271 580 1.13e-30 122
MsG0480021850.01.T01 MTR_2g097390 29.022 317 202 9 13 316 272 578 1.38e-30 122
MsG0480021850.01.T01 MTR_2g097473 26.829 328 221 6 12 327 286 606 1.77e-30 122
MsG0480021850.01.T01 MTR_2g034260 28.105 306 204 7 12 305 207 508 7.29e-30 119
MsG0480021850.01.T01 MTR_3g022830 26.997 363 193 10 16 316 26 378 1.93e-29 117
MsG0480021850.01.T01 MTR_8g442410 28.615 325 206 7 12 317 152 469 2.04e-29 118
MsG0480021850.01.T01 MTR_4g076140 26.234 385 203 10 12 325 45 419 6.81e-28 113
MsG0480021850.01.T01 MTR_7g109580 31.206 282 182 7 20 295 194 469 7.86e-28 114
MsG0480021850.01.T01 MTR_8g093070 30.573 314 187 12 32 327 159 459 1.24e-27 112
MsG0480021850.01.T01 MTR_5g015490 28.743 334 208 9 16 326 115 441 1.53e-27 112
MsG0480021850.01.T01 MTR_2g099110 30.104 289 182 7 14 292 73 351 2.05e-26 108
MsG0480021850.01.T01 MTR_4g026485 30.060 336 186 14 12 325 266 574 4.56e-26 108
MsG0480021850.01.T01 MTR_0092s0100 30.793 328 205 11 8 326 368 682 1.71e-25 107
MsG0480021850.01.T01 MTR_0092s0100 30.793 328 205 11 8 326 365 679 1.79e-25 107
MsG0480021850.01.T01 MTR_5g009080 30.769 234 150 4 12 240 53 279 5.69e-25 105
MsG0480021850.01.T01 MTR_2g089100 27.874 348 209 12 14 324 51 393 5.89e-24 102
MsG0480021850.01.T01 MTR_7g057230 33.205 259 152 10 81 325 358 609 7.35e-21 94.0
MsG0480021850.01.T01 MTR_5g015950 24.377 361 217 10 16 322 36 394 7.13e-19 87.4
MsG0480021850.01.T01 MTR_3g053270 24.377 361 217 10 16 322 36 394 7.13e-19 87.4
MsG0480021850.01.T01 MTR_8g020840 25.868 317 211 9 29 325 185 497 8.04e-15 75.5
MsG0480021850.01.T01 MTR_1g086970 22.867 293 198 9 37 315 130 408 2.72e-14 73.9
MsG0480021850.01.T01 MTR_4g095500 25.068 367 205 11 16 322 60 416 2.84e-14 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021850.01.T01 AT5G66770 62.270 326 113 4 6 325 211 532 8.62e-132 388
MsG0480021850.01.T01 AT3G50650 56.667 330 123 5 6 325 171 490 6.53e-120 356
MsG0480021850.01.T01 AT5G48150 34.783 322 197 8 7 317 114 433 5.68e-50 173
MsG0480021850.01.T01 AT5G48150 34.783 322 197 8 7 317 114 433 5.68e-50 173
MsG0480021850.01.T01 AT5G48150 34.783 322 197 8 7 317 114 433 5.68e-50 173
MsG0480021850.01.T01 AT5G48150 34.783 322 197 8 7 317 114 433 5.68e-50 173
MsG0480021850.01.T01 AT5G41920 33.648 318 184 8 12 315 40 344 5.05e-46 161
MsG0480021850.01.T01 AT2G04890 35.254 295 170 10 34 317 72 356 5.78e-45 158
MsG0480021850.01.T01 AT3G03450 33.945 327 193 12 12 326 180 495 4.26e-44 159
MsG0480021850.01.T01 AT1G50600 33.333 327 206 7 12 328 156 480 7.14e-44 158
MsG0480021850.01.T01 AT1G50600 32.722 327 208 6 12 328 231 555 1.82e-43 158
MsG0480021850.01.T01 AT1G50600 32.722 327 208 6 12 328 227 551 1.85e-43 158
MsG0480021850.01.T01 AT3G54220 33.660 306 180 8 30 325 308 600 3.13e-42 155
MsG0480021850.01.T01 AT1G21450 29.412 323 216 5 14 326 224 544 2.78e-40 149
MsG0480021850.01.T01 AT4G17230 31.498 327 212 6 12 328 155 479 8.79e-40 147
MsG0480021850.01.T01 AT4G17230 31.498 327 212 6 12 328 155 479 8.79e-40 147
MsG0480021850.01.T01 AT5G17490 34.859 284 164 11 51 326 196 466 2.95e-38 142
MsG0480021850.01.T01 AT1G07520 30.032 313 201 6 31 327 170 480 1.87e-37 140
MsG0480021850.01.T01 AT1G07520 30.032 313 201 6 31 327 170 480 1.87e-37 140
MsG0480021850.01.T01 AT3G46600 29.063 320 216 6 16 326 5 322 4.62e-37 137
MsG0480021850.01.T01 AT1G07520 30.032 313 201 6 31 327 335 645 7.78e-37 140
MsG0480021850.01.T01 AT1G07520 30.032 313 201 6 31 327 334 644 8.62e-37 140
MsG0480021850.01.T01 AT4G08250 31.461 267 179 3 35 297 129 395 2.14e-36 137
MsG0480021850.01.T01 AT1G66350 32.492 317 182 13 12 317 152 447 2.32e-36 137
MsG0480021850.01.T01 AT2G01570 33.559 295 171 13 41 325 251 530 2.84e-36 138
MsG0480021850.01.T01 AT1G63100 32.558 258 152 6 78 325 321 566 3.17e-36 138
MsG0480021850.01.T01 AT1G63100 33.333 246 142 6 90 325 358 591 4.05e-36 138
MsG0480021850.01.T01 AT3G46600 29.154 319 215 6 16 325 181 497 1.28e-35 135
MsG0480021850.01.T01 AT3G46600 29.154 319 215 6 16 325 213 529 2.14e-35 135
MsG0480021850.01.T01 AT1G14920 31.987 297 173 12 41 325 199 478 2.61e-35 134
MsG0480021850.01.T01 AT1G07530 27.879 330 218 7 13 327 393 717 7.04e-35 135
MsG0480021850.01.T01 AT2G29065 28.994 338 203 8 16 327 253 579 1.63e-34 133
MsG0480021850.01.T01 AT5G59450 28.440 327 219 6 12 325 224 548 9.25e-34 131
MsG0480021850.01.T01 AT2G29060 27.190 331 224 8 11 327 279 606 1.97e-33 130
MsG0480021850.01.T01 AT2G29060 27.190 331 224 8 11 327 316 643 2.59e-33 130
MsG0480021850.01.T01 AT1G55580 30.488 328 174 8 45 325 74 394 6.93e-33 127
MsG0480021850.01.T01 AT2G37650 28.136 295 192 6 46 327 366 653 4.35e-29 118
MsG0480021850.01.T01 AT2G37650 28.136 295 192 6 46 327 378 665 5.24e-29 117
MsG0480021850.01.T01 AT4G37650 28.528 326 205 9 24 326 158 478 1.24e-28 116
MsG0480021850.01.T01 AT1G50420 31.278 227 139 5 26 246 71 286 1.72e-25 106
MsG0480021850.01.T01 AT5G52510 27.164 335 211 10 14 325 267 591 1.96e-23 101
MsG0480021850.01.T01 AT3G49950 29.630 297 176 11 45 316 60 348 9.90e-22 95.5
MsG0480021850.01.T01 AT4G00150 27.500 320 187 13 27 326 211 505 1.74e-19 89.7
MsG0480021850.01.T01 AT3G60630 28.458 253 156 8 86 325 329 569 3.14e-17 82.8
MsG0480021850.01.T01 AT2G45160 27.378 347 194 15 12 325 268 589 6.19e-17 82.0
MsG0480021850.01.T01 AT3G13840 25.228 329 213 8 14 325 147 459 1.16e-16 80.9

Find 59 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAATAGTGTAAGAATTTC+TGG 0.119456 4:-65154210 MsG0480021850.01.T01:CDS
AATATTCATATAGTTGATTT+TGG 0.139541 4:-65154297 MsG0480021850.01.T01:CDS
CCAGAAATTCTTACACTATT+AGG 0.205458 4:+65154210 None:intergenic
ATATGGGCATGCATCGTTTA+AGG 0.214693 4:+65154380 None:intergenic
GCTTCAGTTGTATAATCTTT+TGG 0.233520 4:-65154001 MsG0480021850.01.T01:CDS
TAAAAGGAAACTCTTTGATT+AGG 0.234171 4:+65154504 None:intergenic
GGTATACCTGCTATGGCTTT+AGG 0.252034 4:-65154189 MsG0480021850.01.T01:CDS
TCACCTAAAGTAACAATCTT+AGG 0.256338 4:+65153925 None:intergenic
AAATGTGCGAACTTTGAATA+TGG 0.257358 4:+65154363 None:intergenic
AAAAGGAAACTCTTTGATTA+GGG 0.285344 4:+65154505 None:intergenic
AATCCTAAGATTGTTACTTT+AGG 0.287104 4:-65153928 MsG0480021850.01.T01:CDS
AGGGTTTCAATTGCTTGATT+TGG 0.313945 4:+65154564 None:intergenic
TTCGATGAGTGAAGCAATTT+CGG 0.323142 4:+65154596 None:intergenic
CAAATTCTTCTTCAACTACT+TGG 0.323189 4:-65154428 MsG0480021850.01.T01:CDS
TAGGGCTTGTGAAAAGTAAA+AGG 0.330479 4:+65154488 None:intergenic
GGATTCGCCGGAGAGGTTTC+AGG 0.345000 4:-65153797 MsG0480021850.01.T01:CDS
TCGATTTACTGAGATGGGTT+AGG 0.351223 4:+65154544 None:intergenic
GCATTTGCAACTCGTTCTTC+TGG 0.357528 4:-65154237 MsG0480021850.01.T01:CDS
TTAACAGCTAAAGCCTCATT+TGG 0.358736 4:+65154030 None:intergenic
ATCAACTATATGAATATTGT+TGG 0.382716 4:+65154302 None:intergenic
CGATTTACTGAGATGGGTTA+GGG 0.403048 4:+65154545 None:intergenic
TTGCTTGGTAGGAGAATTGA+TGG 0.408569 4:-65153763 MsG0480021850.01.T01:CDS
TGAGTGAAGCAATTTCGGTT+AGG 0.410733 4:+65154601 None:intergenic
GAGTGAAGCAATTTCGGTTA+GGG 0.413751 4:+65154602 None:intergenic
GAATTGTTCAAGGGATTCAA+TGG 0.414040 4:-65154275 MsG0480021850.01.T01:CDS
GGCGAATCCAACGCCATGTT+CGG 0.426377 4:+65153811 None:intergenic
GATTCATCAAGAAGCTCAAT+AGG 0.436690 4:+65154069 None:intergenic
TGAAATCGATTTACTGAGAT+GGG 0.451988 4:+65154539 None:intergenic
GTAGCTGAAATCGATGAAAC+TGG 0.464413 4:+65154159 None:intergenic
GTGTGATTGGTGTGAGGGAG+AGG 0.470960 4:-65153741 MsG0480021850.01.T01:CDS
AATTTCTGGTATACCTGCTA+TGG 0.471549 4:-65154196 MsG0480021850.01.T01:CDS
GATTGGTGTGAGGGAGAGGA+TGG 0.472918 4:-65153737 MsG0480021850.01.T01:CDS
AATGTGCGAACTTTGAATAT+GGG 0.473696 4:+65154364 None:intergenic
GTTTCATCGATTTCAGCTAC+TGG 0.480562 4:-65154156 MsG0480021850.01.T01:CDS
GTGAAATCGATTTACTGAGA+TGG 0.489246 4:+65154538 None:intergenic
AGGAGAATTGATGGTGTGAT+TGG 0.505945 4:-65153754 MsG0480021850.01.T01:CDS
TGAGAGTTTGGAACCGAACA+TGG 0.520432 4:-65153824 MsG0480021850.01.T01:CDS
AAATTCTTCTTCAACTACTT+GGG 0.520685 4:-65154427 MsG0480021850.01.T01:CDS
TTTGGAACCGAACATGGCGT+TGG 0.523106 4:-65153818 MsG0480021850.01.T01:CDS
AATTGTTCAAGGGATTCAAT+GGG 0.536168 4:-65154274 MsG0480021850.01.T01:CDS
AGCTCAATAGGCGTAAGAAT+CGG 0.539095 4:+65154081 None:intergenic
TGAAGCTAGTTTGACGACTC+GGG 0.539102 4:-65153899 MsG0480021850.01.T01:CDS
ATGAAGCTAGTTTGACGACT+CGG 0.543619 4:-65153900 MsG0480021850.01.T01:CDS
AGTAAATCGATTTCACAAAA+CGG 0.553570 4:-65154531 MsG0480021850.01.T01:CDS
GAACATGGCGTTGGATTCGC+CGG 0.557782 4:-65153809 MsG0480021850.01.T01:CDS
GCTAGTTTGACGACTCGGGT+TGG 0.562442 4:-65153895 MsG0480021850.01.T01:CDS
GAAGTTCCTAAAGCCATAGC+AGG 0.575286 4:+65154183 None:intergenic
GGATGGAGGATAAAGAACAG+TGG 0.578860 4:-65153720 MsG0480021850.01.T01:CDS
CTCTCCACCTGAAACCTCTC+CGG 0.581104 4:+65153790 None:intergenic
GGAGGATAAAGAACAGTGGA+AGG 0.599058 4:-65153716 MsG0480021850.01.T01:CDS
TGGTGTGAGGGAGAGGATGG+AGG 0.600379 4:-65153734 MsG0480021850.01.T01:CDS
GCAATTCTTGAAGCAACAGA+AGG 0.601796 4:-65154327 MsG0480021850.01.T01:CDS
TGATGGTGTGATTGGTGTGA+GGG 0.605734 4:-65153746 MsG0480021850.01.T01:CDS
AAAGCTGTGGTTTGAATCAG+TGG 0.605908 4:-65153684 MsG0480021850.01.T01:CDS
TTCGCCGGAGAGGTTTCAGG+TGG 0.626303 4:-65153794 MsG0480021850.01.T01:CDS
AAGCTGTGGTTTGAATCAGT+GGG 0.636862 4:-65153683 MsG0480021850.01.T01:CDS
TGGCGTTGGATTCGCCGGAG+AGG 0.656610 4:-65153804 MsG0480021850.01.T01:CDS
TTGATGGTGTGATTGGTGTG+AGG 0.659575 4:-65153747 MsG0480021850.01.T01:CDS
AGAAGCTATTGAAGATTGTG+AGG 0.683841 4:+65154452 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATTCATATAGTTGATTT+TGG - Chr4:65154026-65154045 MsG0480021850.01.T01:CDS 15.0%
!! ATCAACTATATGAATATTGT+TGG + Chr4:65154024-65154043 None:intergenic 20.0%
! AAAAGGAAACTCTTTGATTA+GGG + Chr4:65153821-65153840 None:intergenic 25.0%
! AAATTCTTCTTCAACTACTT+GGG - Chr4:65153896-65153915 MsG0480021850.01.T01:CDS 25.0%
! TAAAAGGAAACTCTTTGATT+AGG + Chr4:65153822-65153841 None:intergenic 25.0%
!! AGTAAATCGATTTCACAAAA+CGG - Chr4:65153792-65153811 MsG0480021850.01.T01:CDS 25.0%
!!! AATCCTAAGATTGTTACTTT+AGG - Chr4:65154395-65154414 MsG0480021850.01.T01:CDS 25.0%
!!! AATTGCTTGATTTGGTTTTT+GGG + Chr4:65153754-65153773 None:intergenic 25.0%
!!! TTGATTTTGGAATTGTTCAA+GGG - Chr4:65154039-65154058 MsG0480021850.01.T01:CDS 25.0%
AAATGTGCGAACTTTGAATA+TGG + Chr4:65153963-65153982 None:intergenic 30.0%
AATGTGCGAACTTTGAATAT+GGG + Chr4:65153962-65153981 None:intergenic 30.0%
AATTGTTCAAGGGATTCAAT+GGG - Chr4:65154049-65154068 MsG0480021850.01.T01:CDS 30.0%
CAAATTCTTCTTCAACTACT+TGG - Chr4:65153895-65153914 MsG0480021850.01.T01:CDS 30.0%
CCAGAAATTCTTACACTATT+AGG + Chr4:65154116-65154135 None:intergenic 30.0%
CCTAATAGTGTAAGAATTTC+TGG - Chr4:65154113-65154132 MsG0480021850.01.T01:CDS 30.0%
TCACCTAAAGTAACAATCTT+AGG + Chr4:65154401-65154420 None:intergenic 30.0%
! GCTTCAGTTGTATAATCTTT+TGG - Chr4:65154322-65154341 MsG0480021850.01.T01:CDS 30.0%
! TGAAATCGATTTACTGAGAT+GGG + Chr4:65153787-65153806 None:intergenic 30.0%
!!! CAATTGCTTGATTTGGTTTT+TGG + Chr4:65153755-65153774 None:intergenic 30.0%
!!! GTTGATTTTGGAATTGTTCA+AGG - Chr4:65154038-65154057 MsG0480021850.01.T01:CDS 30.0%
AGAAGCTATTGAAGATTGTG+AGG + Chr4:65153874-65153893 None:intergenic 35.0%
AGGGTTTCAATTGCTTGATT+TGG + Chr4:65153762-65153781 None:intergenic 35.0%
GAATTGTTCAAGGGATTCAA+TGG - Chr4:65154048-65154067 MsG0480021850.01.T01:CDS 35.0%
GATTCATCAAGAAGCTCAAT+AGG + Chr4:65154257-65154276 None:intergenic 35.0%
TAGGGCTTGTGAAAAGTAAA+AGG + Chr4:65153838-65153857 None:intergenic 35.0%
TTAACAGCTAAAGCCTCATT+TGG + Chr4:65154296-65154315 None:intergenic 35.0%
TTCGATGAGTGAAGCAATTT+CGG + Chr4:65153730-65153749 None:intergenic 35.0%
! AGATTGTGAGGTGATTTTGT+TGG + Chr4:65153862-65153881 None:intergenic 35.0%
! CTTTTGTATTCAGCCAAATG+AGG - Chr4:65154280-65154299 MsG0480021850.01.T01:CDS 35.0%
! GTGAAATCGATTTACTGAGA+TGG + Chr4:65153788-65153807 None:intergenic 35.0%
!! AATTTCTGGTATACCTGCTA+TGG - Chr4:65154127-65154146 MsG0480021850.01.T01:CDS 35.0%
!! TGAGGTGATTTTGTTGGTTA+GGG + Chr4:65153856-65153875 None:intergenic 35.0%
!!! TGCTGCTTTTTTTGAGAGTT+TGG - Chr4:65154487-65154506 MsG0480021850.01.T01:CDS 35.0%
AAAGCTGTGGTTTGAATCAG+TGG - Chr4:65154639-65154658 MsG0480021850.01.T01:CDS 40.0%
AAGCTGTGGTTTGAATCAGT+GGG - Chr4:65154640-65154659 MsG0480021850.01.T01:CDS 40.0%
AGCTCAATAGGCGTAAGAAT+CGG + Chr4:65154245-65154264 None:intergenic 40.0%
ATATGGGCATGCATCGTTTA+AGG + Chr4:65153946-65153965 None:intergenic 40.0%
GAGTGAAGCAATTTCGGTTA+GGG + Chr4:65153724-65153743 None:intergenic 40.0%
GCAATTCTTGAAGCAACAGA+AGG - Chr4:65153996-65154015 MsG0480021850.01.T01:CDS 40.0%
GTAGCTGAAATCGATGAAAC+TGG + Chr4:65154167-65154186 None:intergenic 40.0%
TGAGTGAAGCAATTTCGGTT+AGG + Chr4:65153725-65153744 None:intergenic 40.0%
TTGCTTGGTAGGAGAATTGA+TGG - Chr4:65154560-65154579 MsG0480021850.01.T01:CDS 40.0%
! ATGAAGCTAGTTTGACGACT+CGG - Chr4:65154423-65154442 MsG0480021850.01.T01:CDS 40.0%
! TCGATTTACTGAGATGGGTT+AGG + Chr4:65153782-65153801 None:intergenic 40.0%
!! AAGGTTTTGATGGAAAGCTG+TGG - Chr4:65154626-65154645 MsG0480021850.01.T01:CDS 40.0%
!! AGAACAGTGGAAGGTTTTGA+TGG - Chr4:65154616-65154635 MsG0480021850.01.T01:CDS 40.0%
!! AGGAGAATTGATGGTGTGAT+TGG - Chr4:65154569-65154588 MsG0480021850.01.T01:CDS 40.0%
!! CGATTTACTGAGATGGGTTA+GGG + Chr4:65153781-65153800 None:intergenic 40.0%
!! GTGAGGTGATTTTGTTGGTT+AGG + Chr4:65153857-65153876 None:intergenic 40.0%
!! GTTTCATCGATTTCAGCTAC+TGG - Chr4:65154167-65154186 MsG0480021850.01.T01:CDS 40.0%
!!! CAAACGAAGCGCTTTTTCAA+CGG + Chr4:65154363-65154382 None:intergenic 40.0%
!!! GGGTTTTAAGAAGTGGTTTG+TGG + Chr4:65153704-65153723 None:intergenic 40.0%
!!! TCGGTTAGGGTTTTAAGAAG+TGG + Chr4:65153711-65153730 None:intergenic 40.0%
GAAGTTCCTAAAGCCATAGC+AGG + Chr4:65154143-65154162 None:intergenic 45.0%
GCATTTGCAACTCGTTCTTC+TGG - Chr4:65154086-65154105 MsG0480021850.01.T01:CDS 45.0%
GGAGGATAAAGAACAGTGGA+AGG - Chr4:65154607-65154626 MsG0480021850.01.T01:CDS 45.0%
GGATGGAGGATAAAGAACAG+TGG - Chr4:65154603-65154622 MsG0480021850.01.T01:CDS 45.0%
GGTATACCTGCTATGGCTTT+AGG - Chr4:65154134-65154153 MsG0480021850.01.T01:CDS 45.0%
! TGAAGCTAGTTTGACGACTC+GGG - Chr4:65154424-65154443 MsG0480021850.01.T01:CDS 45.0%
! TGAGAGTTTGGAACCGAACA+TGG - Chr4:65154499-65154518 MsG0480021850.01.T01:CDS 45.0%
! TGATGGTGTGATTGGTGTGA+GGG - Chr4:65154577-65154596 MsG0480021850.01.T01:CDS 45.0%
! TGGAGAGTCTTTTGCTTGGT+AGG - Chr4:65154549-65154568 MsG0480021850.01.T01:CDS 45.0%
!! CGTTGAAAAAGCGCTTCGTT+TGG - Chr4:65154361-65154380 MsG0480021850.01.T01:CDS 45.0%
!! TTGATGGTGTGATTGGTGTG+AGG - Chr4:65154576-65154595 MsG0480021850.01.T01:CDS 45.0%
! CAGGTGGAGAGTCTTTTGCT+TGG - Chr4:65154545-65154564 MsG0480021850.01.T01:CDS 50.0%
! TTTGGAACCGAACATGGCGT+TGG - Chr4:65154505-65154524 MsG0480021850.01.T01:CDS 50.0%
CTCTCCACCTGAAACCTCTC+CGG + Chr4:65154536-65154555 None:intergenic 55.0%
GATTGGTGTGAGGGAGAGGA+TGG - Chr4:65154586-65154605 MsG0480021850.01.T01:CDS 55.0%
GGCGAATCCAACGCCATGTT+CGG + Chr4:65154515-65154534 None:intergenic 55.0%
GTGTGATTGGTGTGAGGGAG+AGG - Chr4:65154582-65154601 MsG0480021850.01.T01:CDS 55.0%
! GAACATGGCGTTGGATTCGC+CGG - Chr4:65154514-65154533 MsG0480021850.01.T01:CDS 55.0%
! GCTAGTTTGACGACTCGGGT+TGG - Chr4:65154428-65154447 MsG0480021850.01.T01:CDS 55.0%
GGATTCGCCGGAGAGGTTTC+AGG - Chr4:65154526-65154545 MsG0480021850.01.T01:CDS 60.0%
TGGTGTGAGGGAGAGGATGG+AGG - Chr4:65154589-65154608 MsG0480021850.01.T01:CDS 60.0%
TTCGCCGGAGAGGTTTCAGG+TGG - Chr4:65154529-65154548 MsG0480021850.01.T01:CDS 60.0%
! TGGCGTTGGATTCGCCGGAG+AGG - Chr4:65154519-65154538 MsG0480021850.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 65153678 65154667 65153678 ID=MsG0480021850.01;Name=MsG0480021850.01
Chr4 mRNA 65153678 65154667 65153678 ID=MsG0480021850.01.T01;Parent=MsG0480021850.01;Name=MsG0480021850.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|329
Chr4 exon 65153678 65154667 65153678 ID=MsG0480021850.01.T01:exon:20600;Parent=MsG0480021850.01.T01
Chr4 CDS 65153678 65154667 65153678 ID=MsG0480021850.01.T01:cds;Parent=MsG0480021850.01.T01
Gene Sequence

>MsG0480021850.01.T01

ATGCTTTGCGTCGTTTTCATCTTCCACAAACCACTTCTTAAAACCCTAACCGAAATTGCTTCACTCATCGAAACCCAAAAACCAAATCAAGCAATTGAAACCCTAACCCATCTCAGTAAATCGATTTCACAAAACGGAAACCCTAATCAAAGAGTTTCCTTTTACTTTTCACAAGCCCTAACCAACAAAATCACCTCACAATCTTCAATAGCTTCTTCAAATTCTTCTTCAACTACTTGGGAAGAGTTAACACTCTCTTACAAAGCCTTAAACGATGCATGCCCATATTCAAAGTTCGCACATTTAACAGCAAATCAAGCAATTCTTGAAGCAACAGAAGGATCCAACAATATTCATATAGTTGATTTTGGAATTGTTCAAGGGATTCAATGGGCTGCATTGTTACAAGCATTTGCAACTCGTTCTTCTGGTAAACCTAATAGTGTAAGAATTTCTGGTATACCTGCTATGGCTTTAGGAACTTCGCCAGTTTCATCGATTTCAGCTACTGGTAATCGTTTATCTGAGTTTGCGAAACTTCTAGAGTTAAATTTTGAGTTTACACCGATTCTTACGCCTATTGAGCTTCTTGATGAATCAAGCTTTTGTATTCAGCCAAATGAGGCTTTAGCTGTTAATTTCATGCTTCAGTTGTATAATCTTTTGGATGAGAATATGAATTCCGTTGAAAAAGCGCTTCGTTTGGCGAAATCATTGAATCCTAAGATTGTTACTTTAGGTGAATATGAAGCTAGTTTGACGACTCGGGTTGGATTTGTTGAAAGATTTGAAACTGCTTTTAATTATTTTGCTGCTTTTTTTGAGAGTTTGGAACCGAACATGGCGTTGGATTCGCCGGAGAGGTTTCAGGTGGAGAGTCTTTTGCTTGGTAGGAGAATTGATGGTGTGATTGGTGTGAGGGAGAGGATGGAGGATAAAGAACAGTGGAAGGTTTTGATGGAAAGCTGTGGTTTGAATCAGTGGGTTTGA
Protein sequence

>MsG0480021850.01.T01

MLCVVFIFHKPLLKTLTEIASLIETQKPNQAIETLTHLSKSISQNGNPNQRVSFYFSQALTNKITSQSSIASSNSSSTTWEELTLSYKALNDACPYSKFAHLTANQAILEATEGSNNIHIVDFGIVQGIQWAALLQAFATRSSGKPNSVRISGIPAMALGTSPVSSISATGNRLSEFAKLLELNFEFTPILTPIELLDESSFCIQPNEALAVNFMLQLYNLLDENMNSVEKALRLAKSLNPKIVTLGEYEASLTTRVGFVERFETAFNYFAAFFESLEPNMALDSPERFQVESLLLGRRIDGVIGVRERMEDKEQWKVLMESCGLNQWV*