AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021851.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021851.01.T01 MTR_6g025550 64.130 92 23 2 119 210 47 128 8.48e-33 118
MsG0480021851.01.T01 MTR_3g005220 61.290 93 31 3 1 92 37 125 6.17e-32 117
MsG0480021851.01.T01 MTR_2g098880 51.515 66 32 0 37 102 59 124 3.46e-15 74.3
MsG0480021851.01.T01 MTR_2g098880 51.515 66 32 0 37 102 71 136 4.89e-15 73.9
MsG0480021851.01.T01 MTR_6g060130 56.452 62 25 2 1 61 62 122 4.37e-14 68.2
MsG0480021851.01.T01 MTR_2g098920 36.905 84 49 1 14 93 51 134 7.41e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021851.01.T01 AT4G38180 34.673 199 106 1 113 287 392 590 2.54e-36 138
MsG0480021851.01.T01 AT2G27110 32.620 187 104 2 117 281 198 384 1.48e-29 119
MsG0480021851.01.T01 AT2G27110 32.620 187 104 2 117 281 343 529 1.56e-29 119
MsG0480021851.01.T01 AT2G27110 32.620 187 104 2 117 281 343 529 1.56e-29 119
MsG0480021851.01.T01 AT5G18960 36.364 176 91 1 127 281 503 678 1.96e-27 112
MsG0480021851.01.T01 AT5G18960 37.500 104 58 2 28 127 28 128 8.17e-13 69.3
MsG0480021851.01.T01 AT5G18960 36.364 176 91 1 127 281 503 678 1.96e-27 112
MsG0480021851.01.T01 AT5G18960 37.500 104 58 2 28 127 28 128 8.17e-13 69.3
MsG0480021851.01.T01 AT3G06250 32.955 176 97 1 127 281 479 654 2.60e-25 106
MsG0480021851.01.T01 AT3G06250 38.261 115 65 2 16 127 2 113 2.35e-15 77.0
MsG0480021851.01.T01 AT3G06250 32.955 176 97 1 127 281 479 654 2.60e-25 106
MsG0480021851.01.T01 AT3G06250 38.261 115 65 2 16 127 2 113 2.35e-15 77.0
MsG0480021851.01.T01 AT3G06250 32.955 176 97 1 127 281 479 654 2.60e-25 106
MsG0480021851.01.T01 AT3G06250 38.261 115 65 2 16 127 2 113 2.35e-15 77.0
MsG0480021851.01.T01 AT4G38170 28.205 195 116 1 117 287 161 355 3.11e-25 105
MsG0480021851.01.T01 AT4G15090 28.649 185 108 1 113 273 368 552 1.38e-21 95.5
MsG0480021851.01.T01 AT4G15090 28.649 185 108 1 113 273 368 552 1.38e-21 95.5
MsG0480021851.01.T01 AT4G15090 28.649 185 108 1 113 273 368 552 1.38e-21 95.5
MsG0480021851.01.T01 AT4G15090 28.649 185 108 1 113 273 368 552 1.38e-21 95.5
MsG0480021851.01.T01 AT4G15090 28.649 185 108 1 113 273 368 552 1.38e-21 95.5
MsG0480021851.01.T01 AT3G22170 28.729 181 105 1 114 270 378 558 3.83e-19 88.2
MsG0480021851.01.T01 AT3G22170 28.729 181 105 1 114 270 378 558 3.83e-19 88.2
MsG0480021851.01.T01 AT1G76320 27.027 185 109 2 114 274 306 488 8.91e-16 78.2
MsG0480021851.01.T01 AT1G76320 27.027 185 109 2 114 274 306 488 9.43e-16 78.2
MsG0480021851.01.T01 AT1G76320 27.027 185 109 2 114 274 306 488 9.43e-16 78.2
MsG0480021851.01.T01 AT1G76320 27.027 185 109 2 114 274 306 488 9.95e-16 78.2
MsG0480021851.01.T01 AT1G52520 30.435 161 80 4 113 245 389 545 1.12e-15 77.8
MsG0480021851.01.T01 AT1G80010 26.738 187 101 3 119 269 409 595 1.35e-15 77.8
MsG0480021851.01.T01 AT4G19990 26.380 163 96 1 110 248 256 418 2.74e-13 70.9
MsG0480021851.01.T01 AT4G19990 26.380 163 96 1 110 248 313 475 3.18e-13 70.5
MsG0480021851.01.T01 AT4G19990 26.380 163 96 1 110 248 313 475 3.18e-13 70.5
MsG0480021851.01.T01 AT4G19990 26.380 163 96 1 110 248 327 489 3.39e-13 70.5
MsG0480021851.01.T01 AT4G19990 26.380 163 96 1 110 248 313 475 3.75e-13 70.5
MsG0480021851.01.T01 AT4G19990 26.380 163 96 1 110 248 313 475 3.86e-13 70.5
MsG0480021851.01.T01 AT4G19990 26.380 163 96 1 110 248 313 475 3.86e-13 70.5
MsG0480021851.01.T01 AT2G32250 24.352 193 119 4 114 281 272 462 8.86e-13 69.3
MsG0480021851.01.T01 AT2G32250 24.352 193 119 4 114 281 272 462 9.02e-13 69.3
MsG0480021851.01.T01 AT2G32250 24.352 193 119 4 114 281 313 503 9.27e-13 69.3
MsG0480021851.01.T01 AT2G32250 24.352 193 119 4 114 281 313 503 9.27e-13 69.3
MsG0480021851.01.T01 AT2G32250 24.352 193 119 4 114 281 313 503 9.27e-13 69.3
MsG0480021851.01.T01 AT2G32250 24.352 193 119 4 114 281 313 503 9.43e-13 68.9
MsG0480021851.01.T01 AT2G32250 24.352 193 119 4 114 281 313 503 9.43e-13 69.3
MsG0480021851.01.T01 AT2G32250 24.352 193 119 4 114 281 313 503 9.43e-13 69.3
MsG0480021851.01.T01 AT1G10240 27.215 158 89 3 114 246 371 527 3.75e-11 64.3
MsG0480021851.01.T01 AT1G10240 27.215 158 89 3 114 246 371 527 3.75e-11 64.3

Find 62 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAATGTTTAGAATATTTC+AGG 0.197001 4:-65162245 MsG0480021851.01.T01:CDS
AATGGTAGTCCGTTATTTAT+AGG 0.222226 4:+65163675 None:intergenic
TTGTTCAAAGGAAGGGTTTC+GGG 0.231331 4:-65163493 MsG0480021851.01.T01:CDS
CATTCCTTCAAAGGGCTCTA+AGG 0.254023 4:+65163644 None:intergenic
CTCTCTTTCCTTGTTGTAAC+GGG 0.308781 4:+65162351 None:intergenic
ACGATACTAGAGATATAGTT+TGG 0.312091 4:-65164820 MsG0480021851.01.T01:five_prime_UTR
AGATGATGGAAACTAGGTTT+AGG 0.322474 4:-65164778 MsG0480021851.01.T01:five_prime_UTR
AGAACAGACGGAATGATAAT+TGG 0.340924 4:-65163528 MsG0480021851.01.T01:CDS
TTTGTCGCAGAAGAAGTTAA+AGG 0.347626 4:-65162199 MsG0480021851.01.T01:CDS
TATACTCCATTCGAGCAAAA+TGG 0.347854 4:-65162518 MsG0480021851.01.T01:CDS
GATGATGGAAACTAGGTTTA+GGG 0.349014 4:-65164777 MsG0480021851.01.T01:five_prime_UTR
ATGTCAAGAGCAGCAGTTTC+AGG 0.356702 4:+65163702 None:intergenic
TAGAAGAATTTGATTCTGAA+TGG 0.406738 4:-65162593 MsG0480021851.01.T01:CDS
CAGATTAAAGATGACAGATA+TGG 0.414073 4:-65163760 MsG0480021851.01.T01:exon
TTTGATGCTTCTTCTTCCAT+TGG 0.427301 4:+65162280 None:intergenic
TCTCTCTTTCCTTGTTGTAA+CGG 0.435704 4:+65162350 None:intergenic
ATATTTCAGGATGAATTAGT+TGG 0.438960 4:-65162232 MsG0480021851.01.T01:CDS
TTTGTTCAAAGGAAGGGTTT+CGG 0.447784 4:-65163494 MsG0480021851.01.T01:CDS
AGAAGAATTTGATTCTGAAT+GGG 0.459561 4:-65162592 MsG0480021851.01.T01:CDS
ATTCCTTCAAAGGGCTCTAA+GGG 0.472871 4:+65163645 None:intergenic
GAAGCAATGATAGATACATA+TGG 0.475667 4:-65162570 MsG0480021851.01.T01:CDS
AGGCAAGGAATCACTACAAA+AGG 0.478706 4:-65163602 MsG0480021851.01.T01:CDS
GGATATAAAGTTCGCAAAAG+TGG 0.480862 4:+65162301 None:intergenic
TGTGAACTTCATTCCTTCAA+AGG 0.481629 4:+65163635 None:intergenic
GTGAACTTCATTCCTTCAAA+GGG 0.487547 4:+65163636 None:intergenic
CCTTGTTGTAACGGGCATCA+AGG 0.494322 4:+65162359 None:intergenic
TTCAGAATCAAATTCTTCTA+CGG 0.510630 4:+65162596 None:intergenic
TTTGCGAACTTTATATCCAA+TGG 0.514843 4:-65162296 MsG0480021851.01.T01:CDS
CGATACTAGAGATATAGTTT+GGG 0.518067 4:-65164819 MsG0480021851.01.T01:five_prime_UTR
CTTGTTGTAACGGGCATCAA+GGG 0.519566 4:+65162360 None:intergenic
ACCTAAAATAGAAAAGATGA+TGG 0.520524 4:-65164792 MsG0480021851.01.T01:five_prime_UTR
GTGATTCCTTGCCTCTTCGA+TGG 0.520594 4:+65163611 None:intergenic
TAAAGAGTTTCTTTGTTCAA+AGG 0.523442 4:-65163505 MsG0480021851.01.T01:CDS
GACATGGATCCTATAAATAA+CGG 0.523874 4:-65163684 MsG0480021851.01.T01:CDS
GAGTTTCTTTGTTCAAAGGA+AGG 0.524715 4:-65163501 MsG0480021851.01.T01:CDS
GTGTCAGACATGATGAAACT+AGG 0.525439 4:-65163437 MsG0480021851.01.T01:CDS
AAATCATGACGTACATAAGC+AGG 0.530663 4:+65162492 None:intergenic
TAGAAAAGATGATGGAAACT+AGG 0.532109 4:-65164784 MsG0480021851.01.T01:five_prime_UTR
AGTTTCTTTGTTCAAAGGAA+GGG 0.533222 4:-65163500 MsG0480021851.01.T01:CDS
ATGGGTTGCAAGATAATCAA+TGG 0.535566 4:-65162551 MsG0480021851.01.T01:CDS
AAGCAATGATAGATACATAT+GGG 0.546295 4:-65162569 MsG0480021851.01.T01:CDS
TAGTGAAGTGTCAACATACA+AGG 0.553752 4:-65162173 MsG0480021851.01.T01:CDS
TGATGGAAACTAGGTTTAGG+GGG 0.554708 4:-65164775 MsG0480021851.01.T01:five_prime_UTR
ATTCTTCTACGGTCGTACAT+AGG 0.554757 4:+65162607 None:intergenic
TAGACATGATTCAAGTACTC+TGG 0.558386 4:+65162675 None:intergenic
TGAAACTGCTGCTCTTGACA+TGG 0.571525 4:-65163700 MsG0480021851.01.T01:CDS
CTGTTCTTGATTTAGTAACA+CGG 0.577898 4:+65163544 None:intergenic
AGTAACACGGCCCATGCGAA+AGG 0.583261 4:+65163557 None:intergenic
AAGCATCAAAAGTTTATACA+AGG 0.598083 4:-65162267 MsG0480021851.01.T01:CDS
TGTCAGACATGATGAAACTA+GGG 0.602315 4:-65163436 MsG0480021851.01.T01:intron
ATCAAAGTCACATAGTAGCT+AGG 0.604390 4:+65162127 None:intergenic
CCTTGATGCCCGTTACAACA+AGG 0.607670 4:-65162359 MsG0480021851.01.T01:CDS
GTTACTAAATCAAGAACAGA+CGG 0.612323 4:-65163540 MsG0480021851.01.T01:CDS
ATGATGGAAACTAGGTTTAG+GGG 0.613803 4:-65164776 MsG0480021851.01.T01:five_prime_UTR
TACATCATCAGAACAAGCCA+TGG 0.620956 4:+65163725 None:intergenic
TCACATCCATCGAAGAGGCA+AGG 0.624553 4:-65163617 MsG0480021851.01.T01:CDS
AAAAGGTACGCTAAGAACAA+AGG 0.638535 4:-65163585 MsG0480021851.01.T01:CDS
TCCTTCAAAGGGCTCTAAGG+GGG 0.640867 4:+65163647 None:intergenic
GAAGTTCACATCCATCGAAG+AGG 0.645982 4:-65163622 MsG0480021851.01.T01:CDS
ATCATCAGAACAAGCCATGG+TGG 0.649034 4:+65163728 None:intergenic
TTCCTTCAAAGGGCTCTAAG+GGG 0.657570 4:+65163646 None:intergenic
TATGGATGAAGATTCCACCA+TGG 0.721549 4:-65163742 MsG0480021851.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAGTATGAATAAATTTTTA+AGG - Chr4:65164456-65164475 MsG0480021851.01.T01:intron 10.0%
!! ATGATAAAATAGAGATTAAT+TGG - Chr4:65162386-65162405 MsG0480021851.01.T01:CDS 15.0%
!! TATGATAAAAAAATAACTAC+TGG + Chr4:65162915-65162934 None:intergenic 15.0%
!! TATTTAAAAAAAAGTGAAGA+TGG - Chr4:65163500-65163519 MsG0480021851.01.T01:CDS 15.0%
!!! TAATTTTCCGTATAAAATTT+GGG + Chr4:65162770-65162789 None:intergenic 15.0%
!!! TTAATTTTCCGTATAAAATT+TGG + Chr4:65162771-65162790 None:intergenic 15.0%
!!! TTTAATTGGATGATTTTAAT+TGG + Chr4:65162449-65162468 None:intergenic 15.0%
!! TAGTAAGAATAATAGAACAA+TGG - Chr4:65162174-65162193 MsG0480021851.01.T01:CDS 20.0%
!! TATTTATTTGTCAAGTATGT+CGG + Chr4:65163949-65163968 None:intergenic 20.0%
!! TTAATCATCATTATTGTATG+TGG - Chr4:65164177-65164196 MsG0480021851.01.T01:intron 20.0%
!!! AAAATGGCATACAAATATTT+TGG - Chr4:65163904-65163923 MsG0480021851.01.T01:intron 20.0%
!!! AAAATTAGCAAATTGCTTTT+GGG - Chr4:65163867-65163886 MsG0480021851.01.T01:intron 20.0%
!!! AATTTTCCGTATAAAATTTG+GGG + Chr4:65162769-65162788 None:intergenic 20.0%
!!! ATATCTCACATTTATTTTCT+GGG - Chr4:65162527-65162546 MsG0480021851.01.T01:CDS 20.0%
!!! ATTTAAAATGCTAGAAACAA+AGG + Chr4:65163089-65163108 None:intergenic 20.0%
!!! CAATTGACAATTTTTAAAGT+AGG + Chr4:65163803-65163822 None:intergenic 20.0%
!!! GAAAATGTTTAGAATATTTC+AGG - Chr4:65164648-65164667 MsG0480021851.01.T01:intron 20.0%
!!! TTTTCTTTTTTTAGCACTAA+TGG + Chr4:65162479-65162498 None:intergenic 20.0%
! AAAAAAAAGTGAAGATGGAT+GGG - Chr4:65163505-65163524 MsG0480021851.01.T01:CDS 25.0%
! AAGAATAATAGAACAATGGA+AGG - Chr4:65162178-65162197 MsG0480021851.01.T01:CDS 25.0%
! AAGCAATGATAGATACATAT+GGG - Chr4:65164324-65164343 MsG0480021851.01.T01:intron 25.0%
! AAGCATCAAAAGTTTATACA+AGG - Chr4:65164626-65164645 MsG0480021851.01.T01:intron 25.0%
! AATAACATCTGTCTTATGTA+CGG - Chr4:65163670-65163689 MsG0480021851.01.T01:CDS 25.0%
! ACCTAAAATAGAAAAGATGA+TGG - Chr4:65162101-65162120 MsG0480021851.01.T01:CDS 25.0%
! AGAAGAATTTGATTCTGAAT+GGG - Chr4:65164301-65164320 MsG0480021851.01.T01:intron 25.0%
! AGACAAAAGCAATTAGAAAA+AGG - Chr4:65163757-65163776 MsG0480021851.01.T01:exon 25.0%
! ATATTTCAGGATGAATTAGT+TGG - Chr4:65164661-65164680 MsG0480021851.01.T01:intron 25.0%
! ATTCATACTTTCACTTCTTT+GGG + Chr4:65164447-65164466 None:intergenic 25.0%
! CAATAATGATGATTAACCTT+TGG + Chr4:65164173-65164192 None:intergenic 25.0%
! GAGAAAATAGTACATGATTA+TGG + Chr4:65163553-65163572 None:intergenic 25.0%
! TAAAAAAAAGTGAAGATGGA+TGG - Chr4:65163504-65163523 MsG0480021851.01.T01:CDS 25.0%
! TAAAGAGTTTCTTTGTTCAA+AGG - Chr4:65163388-65163407 MsG0480021851.01.T01:intron 25.0%
! TAGAAGAATTTGATTCTGAA+TGG - Chr4:65164300-65164319 MsG0480021851.01.T01:intron 25.0%
! TATTCATACTTTCACTTCTT+TGG + Chr4:65164448-65164467 None:intergenic 25.0%
! TGAGAATTGTTATCAAATAG+CGG - Chr4:65162855-65162874 MsG0480021851.01.T01:intron 25.0%
! TTCAGAATCAAATTCTTCTA+CGG + Chr4:65164300-65164319 None:intergenic 25.0%
!! GAAAATTAGCAAATTGCTTT+TGG - Chr4:65163866-65163885 MsG0480021851.01.T01:intron 25.0%
!! GTTTTTCTATGCATTTCAAA+TGG - Chr4:65163834-65163853 MsG0480021851.01.T01:intron 25.0%
!! TCATCAACAATCTATTCTTT+TGG - Chr4:65164005-65164024 MsG0480021851.01.T01:intron 25.0%
!! TCCATCATCTTTTCTATTTT+AGG + Chr4:65162105-65162124 None:intergenic 25.0%
!! TTTTATCATAGTAGTAGTTC+AGG - Chr4:65162925-65162944 MsG0480021851.01.T01:intron 25.0%
!!! AAACTAAAGAGAGTTTTGTT+TGG - Chr4:65164048-65164067 MsG0480021851.01.T01:intron 25.0%
!!! ATACAAATATTTTGGTGATG+TGG - Chr4:65163912-65163931 MsG0480021851.01.T01:intron 25.0%
!!! CATATCTCACATTTATTTTC+TGG - Chr4:65162526-65162545 MsG0480021851.01.T01:CDS 25.0%
!!! GGTTGTTTCAAGATTTTTAA+GGG - Chr4:65163526-65163545 MsG0480021851.01.T01:CDS 25.0%
!!! TGTCACAAGTTTGATTTTTT+GGG - Chr4:65164816-65164835 MsG0480021851.01.T01:five_prime_UTR 25.0%
!!! TTGTCACAAGTTTGATTTTT+TGG - Chr4:65164815-65164834 MsG0480021851.01.T01:five_prime_UTR 25.0%
AATGGTAGTCCGTTATTTAT+AGG + Chr4:65163221-65163240 None:intergenic 30.0%
AATTGTCAATTGAAGAATCC+TGG - Chr4:65163811-65163830 MsG0480021851.01.T01:intron 30.0%
AATTGTTATCAAATAGCGGT+AGG - Chr4:65162859-65162878 MsG0480021851.01.T01:intron 30.0%
ACGATACTAGAGATATAGTT+TGG - Chr4:65162073-65162092 MsG0480021851.01.T01:CDS 30.0%
AGTTTCTTTGTTCAAAGGAA+GGG - Chr4:65163393-65163412 MsG0480021851.01.T01:intron 30.0%
ATTGTCAATTGAAGAATCCT+GGG - Chr4:65163812-65163831 MsG0480021851.01.T01:intron 30.0%
CAGATTAAAGATGACAGATA+TGG - Chr4:65163133-65163152 MsG0480021851.01.T01:intron 30.0%
CGATACTAGAGATATAGTTT+GGG - Chr4:65162074-65162093 MsG0480021851.01.T01:CDS 30.0%
CTGTTCTTGATTTAGTAACA+CGG + Chr4:65163352-65163371 None:intergenic 30.0%
GAAGCAATGATAGATACATA+TGG - Chr4:65164323-65164342 MsG0480021851.01.T01:intron 30.0%
GACATGGATCCTATAAATAA+CGG - Chr4:65163209-65163228 MsG0480021851.01.T01:intron 30.0%
GTTACTAAATCAAGAACAGA+CGG - Chr4:65163353-65163372 MsG0480021851.01.T01:intron 30.0%
TAGGCAAAATAAGATAATCC+TGG - Chr4:65162878-65162897 MsG0480021851.01.T01:intron 30.0%
TCTATCATAGAAGTCATTCA+TGG - Chr4:65162963-65162982 MsG0480021851.01.T01:intron 30.0%
TTTGCGAACTTTATATCCAA+TGG - Chr4:65164597-65164616 MsG0480021851.01.T01:intron 30.0%
! TAAGTAGTTCATTTTATCGC+AGG - Chr4:65163017-65163036 MsG0480021851.01.T01:intron 30.0%
! TGCTCTTATTTATGCAATGA+CGG - Chr4:65162820-65162839 MsG0480021851.01.T01:intron 30.0%
! TTCCAAAACTAGTTTCATAG+AGG - Chr4:65162625-65162644 MsG0480021851.01.T01:CDS 30.0%
!! GGGTTGTTTCAAGATTTTTA+AGG - Chr4:65163525-65163544 MsG0480021851.01.T01:CDS 30.0%
!! TAGAAAAGATGATGGAAACT+AGG - Chr4:65162109-65162128 MsG0480021851.01.T01:CDS 30.0%
!! TTTTGGTTGTGCTTTGTTAT+GGG - Chr4:65164022-65164041 MsG0480021851.01.T01:intron 30.0%
!!! AAGACAGATGTTATTTTGCT+GGG + Chr4:65163665-65163684 None:intergenic 30.0%
!!! CTATGAAACTAGTTTTGGAA+TGG + Chr4:65162625-65162644 None:intergenic 30.0%
!!! TAAGACAGATGTTATTTTGC+TGG + Chr4:65163666-65163685 None:intergenic 30.0%
!!! TTTTTTTAGCACTAATGGTG+AGG + Chr4:65162474-65162493 None:intergenic 30.0%
AAATCATGACGTACATAAGC+AGG + Chr4:65164404-65164423 None:intergenic 35.0%
AGAACAGACGGAATGATAAT+TGG - Chr4:65163365-65163384 MsG0480021851.01.T01:intron 35.0%
ATCAAAGTCACATAGTAGCT+AGG + Chr4:65164769-65164788 None:intergenic 35.0%
ATCTACCTTATGTAGCATGT+AGG + Chr4:65162735-65162754 None:intergenic 35.0%
ATGATGATTAACTCCAGTGA+AGG + Chr4:65163989-65164008 None:intergenic 35.0%
GAGTTTCTTTGTTCAAAGGA+AGG - Chr4:65163392-65163411 MsG0480021851.01.T01:intron 35.0%
GGATATAAAGTTCGCAAAAG+TGG + Chr4:65164595-65164614 None:intergenic 35.0%
GTGAACTTCATTCCTTCAAA+GGG + Chr4:65163260-65163279 None:intergenic 35.0%
TAGACATGATTCAAGTACTC+TGG + Chr4:65164221-65164240 None:intergenic 35.0%
TATACTCCATTCGAGCAAAA+TGG - Chr4:65164375-65164394 MsG0480021851.01.T01:intron 35.0%
TCTACCTTATGTAGCATGTA+GGG + Chr4:65162734-65162753 None:intergenic 35.0%
TGAAATGCATAGAAAAACCC+AGG + Chr4:65163832-65163851 None:intergenic 35.0%
TGATGATTAACTCCAGTGAA+GGG + Chr4:65163988-65164007 None:intergenic 35.0%
TGTCAGACATGATGAAACTA+GGG - Chr4:65163457-65163476 MsG0480021851.01.T01:CDS 35.0%
TGTGAACTTCATTCCTTCAA+AGG + Chr4:65163261-65163280 None:intergenic 35.0%
TTTGATGCTTCTTCTTCCAT+TGG + Chr4:65164616-65164635 None:intergenic 35.0%
TTTGTCGCAGAAGAAGTTAA+AGG - Chr4:65164694-65164713 MsG0480021851.01.T01:intron 35.0%
TTTGTTCAAAGGAAGGGTTT+CGG - Chr4:65163399-65163418 MsG0480021851.01.T01:intron 35.0%
! AAAAGGTACGCTAAGAACAA+AGG - Chr4:65163308-65163327 MsG0480021851.01.T01:intron 35.0%
! AAAGGTTTTACCTTTCGCAT+GGG - Chr4:65163326-65163345 MsG0480021851.01.T01:intron 35.0%
! ATGATGGAAACTAGGTTTAG+GGG - Chr4:65162117-65162136 MsG0480021851.01.T01:CDS 35.0%
! ATGGGTTGCAAGATAATCAA+TGG - Chr4:65164342-65164361 MsG0480021851.01.T01:intron 35.0%
! CGAAGAAACTGACAACTTTT+AGG + Chr4:65163575-65163594 None:intergenic 35.0%
! CGATGACCCCAAATTTTATA+CGG - Chr4:65162760-65162779 MsG0480021851.01.T01:intron 35.0%
! CTAATGGTGAGGTGTTTAAT+TGG + Chr4:65162463-65162482 None:intergenic 35.0%
! GGTTGATTCAAGGTCTAAAA+TGG - Chr4:65163888-65163907 MsG0480021851.01.T01:intron 35.0%
! TAGTGAAGTGTCAACATACA+AGG - Chr4:65164720-65164739 MsG0480021851.01.T01:intron 35.0%
! TCTCTCTTTCCTTGTTGTAA+CGG + Chr4:65164546-65164565 None:intergenic 35.0%
! TGGGCTTACTATTCTGATTT+TGG - Chr4:65162546-65162565 MsG0480021851.01.T01:CDS 35.0%
!! AGATGATGGAAACTAGGTTT+AGG - Chr4:65162115-65162134 MsG0480021851.01.T01:CDS 35.0%
!! AGCCTCTATGAAACTAGTTT+TGG + Chr4:65162630-65162649 None:intergenic 35.0%
!! CTTTTGGTTGTGCTTTGTTA+TGG - Chr4:65164021-65164040 MsG0480021851.01.T01:intron 35.0%
!! GATGATGGAAACTAGGTTTA+GGG - Chr4:65162116-65162135 MsG0480021851.01.T01:CDS 35.0%
!!! ATTGCTTTTGGGTTGATTCA+AGG - Chr4:65163878-65163897 MsG0480021851.01.T01:intron 35.0%
ACCATCTTGATCTGTGGTAA+TGG + Chr4:65164120-65164139 None:intergenic 40.0%
ACCATTACCACAGATCAAGA+TGG - Chr4:65164116-65164135 MsG0480021851.01.T01:intron 40.0%
ACTTGGTTAGAAGCAATGGA+TGG - Chr4:65164080-65164099 MsG0480021851.01.T01:intron 40.0%
AGACATGATGAAACTAGGGT+AGG - Chr4:65163461-65163480 MsG0480021851.01.T01:CDS 40.0%
AGGCAAGGAATCACTACAAA+AGG - Chr4:65163291-65163310 MsG0480021851.01.T01:intron 40.0%
ATTCTTCTACGGTCGTACAT+AGG + Chr4:65164289-65164308 None:intergenic 40.0%
ATTGATGTGCTGCATGATTC+TGG - Chr4:65163632-65163651 MsG0480021851.01.T01:CDS 40.0%
CATACTTTCACTTCTTTGGG+TGG + Chr4:65164444-65164463 None:intergenic 40.0%
GATAGAACCATCTTGATCTG+TGG + Chr4:65164126-65164145 None:intergenic 40.0%
GCATTTCAAATGGATGCTGA+AGG - Chr4:65163844-65163863 MsG0480021851.01.T01:intron 40.0%
GTGTCAGACATGATGAAACT+AGG - Chr4:65163456-65163475 MsG0480021851.01.T01:CDS 40.0%
TACATCATCAGAACAAGCCA+TGG + Chr4:65163171-65163190 None:intergenic 40.0%
TAGTAGTAGTTCAGGAGTAG+TGG - Chr4:65162933-65162952 MsG0480021851.01.T01:intron 40.0%
TATGCAATGACGGCTATGAA+TGG - Chr4:65162830-65162849 MsG0480021851.01.T01:intron 40.0%
TATGGATGAAGATTCCACCA+TGG - Chr4:65163151-65163170 MsG0480021851.01.T01:intron 40.0%
TCTGTCTTATGTACGGAAAG+TGG - Chr4:65163677-65163696 MsG0480021851.01.T01:CDS 40.0%
TGATGGAAACTAGGTTTAGG+GGG - Chr4:65162118-65162137 MsG0480021851.01.T01:CDS 40.0%
TTGTTCAAAGGAAGGGTTTC+GGG - Chr4:65163400-65163419 MsG0480021851.01.T01:intron 40.0%
TTTATCGCAGGAGTAGTTCA+TGG - Chr4:65163029-65163048 MsG0480021851.01.T01:intron 40.0%
! AGGAATCCATTTTGCTCGAA+TGG + Chr4:65164384-65164403 None:intergenic 40.0%
! ATTCCTTCAAAGGGCTCTAA+GGG + Chr4:65163251-65163270 None:intergenic 40.0%
! CAAAGGTTTTACCTTTCGCA+TGG - Chr4:65163325-65163344 MsG0480021851.01.T01:intron 40.0%
! CTCTCTTTCCTTGTTGTAAC+GGG + Chr4:65164545-65164564 None:intergenic 40.0%
! GTACGTCATGATTTTTGTGC+TGG - Chr4:65164410-65164429 MsG0480021851.01.T01:intron 40.0%
!! AGTGGTGGTGTTGATAACAT+TGG - Chr4:65163695-65163714 MsG0480021851.01.T01:CDS 40.0%
!! TGATCTGTGGTAATGGTTTC+AGG + Chr4:65164113-65164132 None:intergenic 40.0%
ACTCCAGTGAAGGGAACAAA+CGG + Chr4:65163979-65163998 None:intergenic 45.0%
ATCATCAGAACAAGCCATGG+TGG + Chr4:65163168-65163187 None:intergenic 45.0%
ATGTCAAGAGCAGCAGTTTC+AGG + Chr4:65163194-65163213 None:intergenic 45.0%
CATTCCTTCAAAGGGCTCTA+AGG + Chr4:65163252-65163271 None:intergenic 45.0%
CTACTGGTATTCAACATGCC+AGG + Chr4:65162899-65162918 None:intergenic 45.0%
GAAGGTAGTGAGATCACTAG+AGG - Chr4:65162196-65162215 MsG0480021851.01.T01:CDS 45.0%
GAAGTTCACATCCATCGAAG+AGG - Chr4:65163271-65163290 MsG0480021851.01.T01:intron 45.0%
GCGTACTTGGTTAGAAGCAA+TGG - Chr4:65164076-65164095 MsG0480021851.01.T01:intron 45.0%
GTCTTATGTACGGAAAGTGG+TGG - Chr4:65163680-65163699 MsG0480021851.01.T01:CDS 45.0%
TGAAACTAGGGTAGGATGCA+AGG - Chr4:65163469-65163488 MsG0480021851.01.T01:CDS 45.0%
TGAAACTGCTGCTCTTGACA+TGG - Chr4:65163193-65163212 MsG0480021851.01.T01:intron 45.0%
TGGTCCCTACATGCTACATA+AGG - Chr4:65162727-65162746 MsG0480021851.01.T01:intron 45.0%
TTGTTTGGTTGCTGCGTACT+TGG - Chr4:65164063-65164082 MsG0480021851.01.T01:intron 45.0%
! TGTGTCACAAACTTGCTCGA+TGG + Chr4:65164502-65164521 None:intergenic 45.0%
!! CTTGTTGTAACGGGCATCAA+GGG + Chr4:65164536-65164555 None:intergenic 45.0%
!! GTGCTGCATGATTCTGGTTT+AGG - Chr4:65163638-65163657 MsG0480021851.01.T01:CDS 45.0%
!! TTCCTTCAAAGGGCTCTAAG+GGG + Chr4:65163250-65163269 None:intergenic 45.0%
CCTTGATGCCCGTTACAACA+AGG - Chr4:65164534-65164553 MsG0480021851.01.T01:intron 50.0%
CGCAGGAGTAGTTCATGGAT+AGG - Chr4:65163034-65163053 MsG0480021851.01.T01:intron 50.0%
GGTAGTGAGATCACTAGAGG+TGG - Chr4:65162199-65162218 MsG0480021851.01.T01:CDS 50.0%
GTGATTCCTTGCCTCTTCGA+TGG + Chr4:65163285-65163304 None:intergenic 50.0%
TATCACGTATGCGATCGCGA+AGG - Chr4:65164142-65164161 MsG0480021851.01.T01:intron 50.0%
TCACATCCATCGAAGAGGCA+AGG - Chr4:65163276-65163295 MsG0480021851.01.T01:intron 50.0%
! ATGCCGTTTGTTCCCTTCAC+TGG - Chr4:65163973-65163992 MsG0480021851.01.T01:intron 50.0%
!! CCTTGTTGTAACGGGCATCA+AGG + Chr4:65164537-65164556 None:intergenic 50.0%
!! GATCGCGAAGGTGTTTCCAA+AGG - Chr4:65164154-65164173 MsG0480021851.01.T01:intron 50.0%
!! TCCTTCAAAGGGCTCTAAGG+GGG + Chr4:65163249-65163268 None:intergenic 50.0%
AGTAACACGGCCCATGCGAA+AGG + Chr4:65163339-65163358 None:intergenic 55.0%
ATGTAGGGACCAGCCTTGGA+GGG + Chr4:65162719-65162738 None:intergenic 55.0%
TAGCATGTAGGGACCAGCCT+TGG + Chr4:65162723-65162742 None:intergenic 55.0%
TCCCCCTTAGAGCCCTTTGA+AGG - Chr4:65163245-65163264 MsG0480021851.01.T01:intron 55.0%
!! GGCTCTAAGGGGGACTCTAA+TGG + Chr4:65163239-65163258 None:intergenic 55.0%
CATGTAGGGACCAGCCTTGG+AGG + Chr4:65162720-65162739 None:intergenic 60.0%
TGTAGGGACCAGCCTTGGAG+GGG + Chr4:65162718-65162737 None:intergenic 60.0%
GTAGGGACCAGCCTTGGAGG+GGG + Chr4:65162717-65162736 None:intergenic 65.0%
AGCACCACCCCCTCCAAGGC+TGG - Chr4:65162707-65162726 MsG0480021851.01.T01:intron 70.0%
AGCCTTGGAGGGGGTGGTGC+TGG + Chr4:65162708-65162727 None:intergenic 70.0%
CTCCAGCACCACCCCCTCCA+AGG - Chr4:65162703-65162722 MsG0480021851.01.T01:intron 70.0%
! CTTGGAGGGGGTGGTGCTGG+AGG + Chr4:65162705-65162724 None:intergenic 70.0%
GGGACCAGCCTTGGAGGGGG+TGG + Chr4:65162714-65162733 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr4 gene 65162054 65164861 65162054 ID=MsG0480021851.01;Name=MsG0480021851.01
Chr4 mRNA 65162054 65164861 65162054 ID=MsG0480021851.01.T01;Parent=MsG0480021851.01;Name=MsG0480021851.01.T01;_AED=0.36;_eAED=0.36;_QI=129|0|0|0.75|1|1|4|0|305
Chr4 exon 65164740 65164861 65164740 ID=MsG0480021851.01.T01:exon:20604;Parent=MsG0480021851.01.T01
Chr4 exon 65163437 65163779 65163437 ID=MsG0480021851.01.T01:exon:20603;Parent=MsG0480021851.01.T01
Chr4 exon 65162480 65162706 65162480 ID=MsG0480021851.01.T01:exon:20602;Parent=MsG0480021851.01.T01
Chr4 exon 65162054 65162408 65162054 ID=MsG0480021851.01.T01:exon:20601;Parent=MsG0480021851.01.T01
Chr4 five_prime_UTR 65164740 65164861 65164740 ID=MsG0480021851.01.T01:five_prime_utr;Parent=MsG0480021851.01.T01
Chr4 five_prime_UTR 65163773 65163779 65163773 ID=MsG0480021851.01.T01:five_prime_utr;Parent=MsG0480021851.01.T01
Chr4 CDS 65163437 65163772 65163437 ID=MsG0480021851.01.T01:cds;Parent=MsG0480021851.01.T01
Chr4 CDS 65162480 65162706 65162480 ID=MsG0480021851.01.T01:cds;Parent=MsG0480021851.01.T01
Chr4 CDS 65162054 65162408 65162054 ID=MsG0480021851.01.T01:cds;Parent=MsG0480021851.01.T01
Gene Sequence

>MsG0480021851.01.T01

ATGACAGATATGGATGAAGATTCCACCATGGCTTGTTCTGATGATGTACCTGAAACTGCTGCTCTTGACATGGATCCTATAAATAACGGACTACCATTAGAGTCCCCCTTAGAGCCCTTTGAAGGAATGAAGTTCACATCCATCGAAGAGGCAAGGAATCACTACAAAAGGTACGCTAAGAACAAAGGTTTTACCTTTCGCATGGGCCGTGTTACTAAATCAAGAACAGACGGAATGATAATTGGTAAAGAGTTTCTTTGTTCAAAGGAAGGGTTTCGGGCAAAAAAATATGCAAAGAAAGAAAATAGCAGTCGTGTCAGACATGATGAAACTAGGAAAAAAGTTCCAGAGTACTTGAATCATGTCTATCACGAACATAGTGAATTTAAAAATCAGTTTTACAAGTGCATTCACCTATGTACGACCGTAGAAGAATTTGATTCTGAATGGGAAGCAATGATAGATACATATGGGTTGCAAGATAATCAATGGTTAAAGACGATATACTCCATTCGAGCAAAATGGATTCCTGCTTATGTACGTCATGATTTTTGTGCTGGAATCAAGTTTGTGACACAATATGATAAAGCCCTTGATGCCCGTTACAACAAGGAAAGAGAGAAGACTTTCAAGACAAGAAATTCAAAGCCACTTTTGCGAACTTTATATCCAATGGAAGAAGAAGCATCAAAAGTTTATACAAGGAAAATGTTTAGAATATTTCAGGATGAATTAGTTGGCTCTCAAATATTTGTCGCAGAAGAAGTTAAAGGTTCTAGTGAAGTGTCAACATACAAGGTTCATGAATTTTATAAAGAGAAGCCTAGCTACTATGTGACTTTGATGTTACTTCAAACGAAGCAACTTGTAGTTGTCACAAGTTTGATTTTTTGGGTATTCTTTGTAGACATGTCTTAA
Protein sequence

>MsG0480021851.01.T01

MTDMDEDSTMACSDDVPETAALDMDPINNGLPLESPLEPFEGMKFTSIEEARNHYKRYAKNKGFTFRMGRVTKSRTDGMIIGKEFLCSKEGFRAKKYAKKENSSRVRHDETRKKVPEYLNHVYHEHSEFKNQFYKCIHLCTTVEEFDSEWEAMIDTYGLQDNQWLKTIYSIRAKWIPAYVRHDFCAGIKFVTQYDKALDARYNKEREKTFKTRNSKPLLRTLYPMEEEASKVYTRKMFRIFQDELVGSQIFVAEEVKGSSEVSTYKVHEFYKEKPSYYVTLMLLQTKQLVVVTSLIFWVFFVDMS*