AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021886.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021886.01.T01 MTR_4g123470 89.697 165 17 0 1 165 61 225 1.84e-110 313
MsG0480021886.01.T01 MTR_7g113060 83.784 37 6 0 127 163 3 39 2.33e-15 70.5
MsG0480021886.01.T01 MTR_7g025150 28.000 150 108 0 2 151 59 208 1.67e-14 68.6
MsG0480021886.01.T01 MTR_4g085790 26.667 150 110 0 2 151 59 208 2.25e-13 65.9
MsG0480021886.01.T01 MTR_1g090753 39.437 71 43 0 2 72 76 146 3.46e-13 64.3
MsG0480021886.01.T01 MTR_1g054495 30.519 154 98 4 2 152 59 206 8.56e-13 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 35 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGTTCCTATCGTTGGATA+TGG 0.258835 4:-65575822 MsG0480021886.01.T01:CDS
AATCGTGATGTTTCACTCTA+AGG 0.331454 4:+65575670 None:intergenic
AGGAATTTGCCAAAAGTATC+AGG 0.344083 4:+65575690 None:intergenic
TAGGAACTACCTGATACTTT+TGG 0.356273 4:-65575699 MsG0480021886.01.T01:CDS
CGATCATTCTACGAGTTTAG+TGG 0.373999 4:-65575978 MsG0480021886.01.T01:CDS
ATCTACTTCCACCAAGGTAC+TGG 0.428620 4:+65575937 None:intergenic
ACCTTTGAAGTTCCTATCGT+TGG 0.439777 4:-65575828 MsG0480021886.01.T01:CDS
CGAACATCACATTTGGTATG+TGG 0.461685 4:+65575586 None:intergenic
TGCCGCACTCATGGAATGAT+AGG 0.462626 4:+65575866 None:intergenic
TTGTGCTGCCAATGGTATTG+AGG 0.463000 4:-65575544 MsG0480021886.01.T01:CDS
TGTTCAACAAATCAGTTTGT+CGG 0.470850 4:-65576031 MsG0480021886.01.T01:CDS
CACATACCAAATGTGATGTT+CGG 0.472080 4:-65575585 MsG0480021886.01.T01:CDS
CCAAATGTGATGTTCGGAAA+AGG 0.475440 4:-65575579 MsG0480021886.01.T01:CDS
GTCATGTGTTTATCAACGCT+TGG 0.483566 4:+65575461 None:intergenic
ACATGATGCTGCCGCACTCA+TGG 0.492689 4:+65575857 None:intergenic
TGTGCTGCCAATGGTATTGA+GGG 0.501205 4:-65575543 MsG0480021886.01.T01:CDS
ACAGGAATTATATTGTCATA+AGG 0.502590 4:+65575630 None:intergenic
ATTATTGCTTTCCAGTACCT+TGG 0.503165 4:-65575948 MsG0480021886.01.T01:CDS
GGCCGTTGTTGACAGATGGT+TGG 0.512157 4:-65576010 MsG0480021886.01.T01:CDS
ATTGCTTTCCAGTACCTTGG+TGG 0.549152 4:-65575945 MsG0480021886.01.T01:CDS
TCTGTATATTTGAGTCTCAC+AGG 0.555272 4:+65575612 None:intergenic
TGTCGGCCGTTGTTGACAGA+TGG 0.563208 4:-65576014 MsG0480021886.01.T01:CDS
GTCTCCGCCCTCAATACCAT+TGG 0.581950 4:+65575536 None:intergenic
TTTCAGGTACAAGTGCGTCA+TGG 0.593047 4:-65575505 MsG0480021886.01.T01:CDS
ATGTGATGTTCGGAAAAGGT+TGG 0.606400 4:-65575575 MsG0480021886.01.T01:CDS
TCCAACGATAGGAACTTCAA+AGG 0.611311 4:+65575827 None:intergenic
GCCGCACTCATGGAATGATA+GGG 0.629158 4:+65575867 None:intergenic
GTCGATCCATATCCAACGAT+AGG 0.629633 4:+65575816 None:intergenic
CACGATTACATCATGTTGTG+CGG 0.638431 4:-65575654 MsG0480021886.01.T01:CDS
ACCCTATCATTCCATGAGTG+CGG 0.640367 4:-65575868 MsG0480021886.01.T01:CDS
GCACTCATGGAATGATAGGG+TGG 0.649652 4:+65575870 None:intergenic
CTCCAACCATCTGTCAACAA+CGG 0.679074 4:+65576008 None:intergenic
TTTGAAATCTACTTCCACCA+AGG 0.681040 4:+65575931 None:intergenic
TGGATCGACGAAATCACCGA+AGG 0.700849 4:-65575802 MsG0480021886.01.T01:intron
GCTGCCAATGGTATTGAGGG+CGG 0.739556 4:-65575540 MsG0480021886.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTCATTAACATTTTTTTTTT+AGG - Chr4:65575787-65575806 MsG0480021886.01.T01:intron 15.0%
!! AAAAAACAATAAACAACCTT+CGG + Chr4:65575722-65575741 None:intergenic 20.0%
! ACAGGAATTATATTGTCATA+AGG + Chr4:65575878-65575897 None:intergenic 25.0%
TGTTCAACAAATCAGTTTGT+CGG - Chr4:65575474-65575493 MsG0480021886.01.T01:CDS 30.0%
AATCGTGATGTTTCACTCTA+AGG + Chr4:65575838-65575857 None:intergenic 35.0%
ATTATTGCTTTCCAGTACCT+TGG - Chr4:65575557-65575576 MsG0480021886.01.T01:CDS 35.0%
CACATACCAAATGTGATGTT+CGG - Chr4:65575920-65575939 MsG0480021886.01.T01:CDS 35.0%
TCTGTATATTTGAGTCTCAC+AGG + Chr4:65575896-65575915 None:intergenic 35.0%
! TAGGAACTACCTGATACTTT+TGG - Chr4:65575806-65575825 MsG0480021886.01.T01:CDS 35.0%
! TTTGAAATCTACTTCCACCA+AGG + Chr4:65575577-65575596 None:intergenic 35.0%
!! AGGAATTTGCCAAAAGTATC+AGG + Chr4:65575818-65575837 None:intergenic 35.0%
ATGTGATGTTCGGAAAAGGT+TGG - Chr4:65575930-65575949 MsG0480021886.01.T01:CDS 40.0%
CACGATTACATCATGTTGTG+CGG - Chr4:65575851-65575870 MsG0480021886.01.T01:CDS 40.0%
CCAAATGTGATGTTCGGAAA+AGG - Chr4:65575926-65575945 MsG0480021886.01.T01:CDS 40.0%
CGAACATCACATTTGGTATG+TGG + Chr4:65575922-65575941 None:intergenic 40.0%
CGATCATTCTACGAGTTTAG+TGG - Chr4:65575527-65575546 MsG0480021886.01.T01:CDS 40.0%
GAAGTTCCTATCGTTGGATA+TGG - Chr4:65575683-65575702 MsG0480021886.01.T01:CDS 40.0%
TCCAACGATAGGAACTTCAA+AGG + Chr4:65575681-65575700 None:intergenic 40.0%
! CCTTTTCCGAACATCACATT+TGG + Chr4:65575929-65575948 None:intergenic 40.0%
!! ACCTTTGAAGTTCCTATCGT+TGG - Chr4:65575677-65575696 MsG0480021886.01.T01:CDS 40.0%
ACCCTATCATTCCATGAGTG+CGG - Chr4:65575637-65575656 MsG0480021886.01.T01:CDS 45.0%
ATCTACTTCCACCAAGGTAC+TGG + Chr4:65575571-65575590 None:intergenic 45.0%
ATTGCTTTCCAGTACCTTGG+TGG - Chr4:65575560-65575579 MsG0480021886.01.T01:CDS 45.0%
CTCCAACCATCTGTCAACAA+CGG + Chr4:65575500-65575519 None:intergenic 45.0%
GTCGATCCATATCCAACGAT+AGG + Chr4:65575692-65575711 None:intergenic 45.0%
TTGTGCTGCCAATGGTATTG+AGG - Chr4:65575961-65575980 MsG0480021886.01.T01:CDS 45.0%
! ACGAGTTTTTGTGCTGCCAA+TGG - Chr4:65575953-65575972 MsG0480021886.01.T01:CDS 45.0%
! TGTGCTGCCAATGGTATTGA+GGG - Chr4:65575962-65575981 MsG0480021886.01.T01:CDS 45.0%
! TTTCAGGTACAAGTGCGTCA+TGG - Chr4:65576000-65576019 MsG0480021886.01.T01:CDS 45.0%
GCACTCATGGAATGATAGGG+TGG + Chr4:65575638-65575657 None:intergenic 50.0%
GCCGCACTCATGGAATGATA+GGG + Chr4:65575641-65575660 None:intergenic 50.0%
TGCCGCACTCATGGAATGAT+AGG + Chr4:65575642-65575661 None:intergenic 50.0%
TGGATCGACGAAATCACCGA+AGG - Chr4:65575703-65575722 MsG0480021886.01.T01:intron 50.0%
!!! GCGGAGACGTGTTGATTTTC+AGG - Chr4:65575984-65576003 MsG0480021886.01.T01:CDS 50.0%
ACATGATGCTGCCGCACTCA+TGG + Chr4:65575651-65575670 None:intergenic 55.0%
GGCCGTTGTTGACAGATGGT+TGG - Chr4:65575495-65575514 MsG0480021886.01.T01:CDS 55.0%
GTCTCCGCCCTCAATACCAT+TGG + Chr4:65575972-65575991 None:intergenic 55.0%
TGTCGGCCGTTGTTGACAGA+TGG - Chr4:65575491-65575510 MsG0480021886.01.T01:CDS 55.0%
! GCTGCCAATGGTATTGAGGG+CGG - Chr4:65575965-65575984 MsG0480021886.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 65575473 65576054 65575473 ID=MsG0480021886.01;Name=MsG0480021886.01
Chr4 mRNA 65575473 65576054 65575473 ID=MsG0480021886.01.T01;Parent=MsG0480021886.01;Name=MsG0480021886.01.T01;_AED=0.08;_eAED=0.08;_QI=0|0|0|1|1|1|2|0|165
Chr4 exon 65575473 65575718 65575473 ID=MsG0480021886.01.T01:exon:7969;Parent=MsG0480021886.01.T01
Chr4 exon 65575803 65576054 65575803 ID=MsG0480021886.01.T01:exon:7968;Parent=MsG0480021886.01.T01
Chr4 CDS 65575803 65576054 65575803 ID=MsG0480021886.01.T01:cds;Parent=MsG0480021886.01.T01
Chr4 CDS 65575473 65575718 65575473 ID=MsG0480021886.01.T01:cds;Parent=MsG0480021886.01.T01
Gene Sequence

>MsG0480021886.01.T01

ATGTTCAACAAATCAGTTTGTCGGCCGTTGTTGACAGATGGTTGGAGTGCATTGCGATCATTCTACGAGTTTAGTGGAAGTAAGATTATTGCTTTCCAGTACCTTGGTGGAAGTAGATTTCAAATCTTTGTTTTTGCTCATAACATTACACTAGCTGATTATCCACCCTATCATTCCATGAGTGCGGCAGCATCATGTTATTGTACCTTTGAAGTTCCTATCGTTGGATATGGATCGACGAAATCACCGAAGGAACTACCTGATACTTTTGGCAAATTCCTTAGAGTGAAACATCACGATTACATCATGTTGTGCGGACCTTATGACAATATAATTCCTGTGAGACTCAAATATACAGACGACCACATACCAAATGTGATGTTCGGAAAAGGTTGGACGAGTTTTTGTGCTGCCAATGGTATTGAGGGCGGAGACGTGTTGATTTTCAGGTACAAGTGCGTCATGGACAAAAAATATGTTATCGTATCCAAGCGTTGA
Protein sequence

>MsG0480021886.01.T01

MFNKSVCRPLLTDGWSALRSFYEFSGSKIIAFQYLGGSRFQIFVFAHNITLADYPPYHSMSAAASCYCTFEVPIVGYGSTKSPKELPDTFGKFLRVKHHDYIMLCGPYDNIIPVRLKYTDDHIPNVMFGKGWTSFCAANGIEGGDVLIFRYKCVMDKKYVIVSKR*